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3 results on '"Wataru Tokuhara"'

1. Direct Ethanol Production from Ionic Liquid-Pretreated Lignocellulosic Biomass by Cellulase-Displaying Yeasts

Author

Nobuhiro Ishida, Kazunori Nakashima, Ryosuke Yamada, Akihiko Kondo, Wataru Tokuhara, Noriho Kamiya, Satoshi Katahira, Nanami Asai-Nakashima, and Chiaki Ogino

Subjects
0106 biological sciences, Ionic Liquids, Lignocellulosic biomass, Biomass, Bioengineering, Saccharomyces cerevisiae, Cellulase, Ethanol fermentation, Lignin, 01 natural sciences, Applied Microbiology and Biotechnology, Biochemistry, Fungal Proteins, 010608 biotechnology, Botany, Cellulases, Organic chemistry, Ethanol fuel, Cellulose, Cedrus, Molecular Biology, Trichoderma, Eucalyptus, Xylose, Ethanol, biology, 010405 organic chemistry, Chemistry, Imidazoles, food and beverages, General Medicine, 0104 chemical sciences, Kinetics, Aspergillus, Glucose, Biofuels, Bioconversion of biomass to mixed alcohol fuels, Fermentation, biology.protein, Bagasse, Biotechnology
Abstract

Among the many types of lignocellulosic biomass pretreatment methods, the use of ionic liquids (ILs) is regarded as one of the most promising strategies. In this study, the effects of four kinds of ILs for pretreatment of lignocellulosic biomass such as bagasse, eucalyptus, and cedar were evaluated. In direct ethanol fermentation from biomass incorporated with ILs by cellulase-displaying yeast, 1-butyl-3-methylimidazolium acetate ([Bmim][OAc]) was the most effective IL. The ethanol production and yield from [Bmim][OAc]-pretreated bagasse reached 0.81 g/L and 73.4% of the theoretical yield after fermentation for 96 h. The results prove the initial concept, in which the direct fermentation from lignocellulosic biomass effectively promoted by the pretreatment with IL.

Published
2016

2. Low melting point pyridinium ionic liquid pretreatment for enhancing enzymatic saccharification of cellulosic biomass

Author

Noriho Kamiya, Uju, Aya Nakamoto, Yoshiyuki Noritake, Chiaki Ogino, Wataru Tokuhara, Satoshi Katahira, Yasuhiro Shoda, Nobuhiro Ishida, and Masahiro Goto

Subjects
Environmental Engineering, Ionic Liquids, Bioengineering, Pyridinium Compounds, Cellulase, Chloride, Lignin, chemistry.chemical_compound, Hydrolysis, medicine, Organic chemistry, Transition Temperature, Recycling, Biomass, Cellulose, Waste Management and Disposal, biology, Renewable Energy, Sustainability and the Environment, Chemistry, General Medicine, Microcrystalline cellulose, Cellulosic ethanol, Ionic liquid, biology.protein, Carbohydrate Metabolism, Bagasse, medicine.drug
Abstract

The potential of 1-hexylpyridinium chloride ([Hpy][Cl]), to pretreat cellulosic feedstocks was investigated using microcrystalline cellulose (Avicel) and Bagasse at 80 °C or 100 °C. Short [Hpy][Cl] pretreatments

Published
2012

3. Short time ionic liquids pretreatment on lignocellulosic biomass to enhance enzymatic saccharification

Author

Yoshiyuki Noritake, Aya Nakamoto, Uju, Yasuhiro Shoda, Kazunori Nakashima, Chiaki Ogino, Masahiro Goto, Noriho Kamiya, Wataru Tokuhara, Satoshi Katahira, and Nobuhiro Ishida

Subjects
Environmental Engineering, Time Factors, Biomass, Lignocellulosic biomass, Ionic Liquids, Bioengineering, Cellulase, complex mixtures, Lignin, chemistry.chemical_compound, Hydrolysis, Crystallinity, X-Ray Diffraction, Spectroscopy, Fourier Transform Infrared, Organic chemistry, Cellulose, Waste Management and Disposal, biology, Renewable Energy, Sustainability and the Environment, Depolymerization, Imidazoles, General Medicine, chemistry, Ionic liquid, biology.protein, Carbohydrate Metabolism, Crystallization, Biotechnology
Abstract

The potential of 1-buthyl-3-methylpyridinium chloride, [Bmpy][Cl], as a pretreatment solvent for lignocellulosic biomasses, Bagasse and Eucalyptus, was investigated. The yields of regenerated biomasses ranged between 35% and 96%, and varied according to the pretreatment time, type of ionic liquid (IL) and biomass. The pretreatment of the biomass with [Bmpy][Cl] resulted in up to 8-fold increase in the cellulose conversion when compared with the untreated biomass. For a short pretreatment period (i.e., 10 min), [Bmpy][Cl] showed better performance than 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]) with respect to the initial enzymatic saccharification rates. The increase in the reaction rates with [Emim][OAc] treatment was because of a reduction in the cellulose crystallinity. In contrast, a decrease in the crystallinity index was not clearly observed for the biomass pretreated with [Bmpy][Cl], and the enhancement of the enzymatic saccharification rates using this IL is presumably due to a reduction in the degree of polymerization of cellulose in the biomass.

Published
2011

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