23 results on '"Washino T"'
Search Results
2. Delayed recovery of β-glucuronidase activity driven by an Arabidopsis heat shock promoter in heat-stressed transgenic Nicotiana plumbaginifolia
- Author
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Moriwaki, M., Yamakawa, T., Washino, T., Kodama, T., and Igarashi, Y.
- Published
- 1999
- Full Text
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3. Micro-tensile bond strength of ‘universal’ adhesives to dentin
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Washino, T., primary, Barreto, B.C.F., additional, Van Ende, A., additional, De Munck, J., additional, and Van Meerbeek, B., additional
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- 2014
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4. Metabolism and disposition of (RS)-2-methoxy-3-(octadecylcarbamoyloxy)propyl 2-(3-thiazolio)ethyl phosphate (MOTP) in rats and dogs
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Kobayashi, T., Ohtsuki, T., Esumi, Y., Hohnoki, H., Tanayama, S., and Washino, T.
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PLATELET activating factor ,METABOLISM - Published
- 1988
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5. Two cases of systemic capillary leak syndrome associated with COVID-19 in Japan.
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Kosaka A, Goto T, Washino T, Sakamoto N, Iwabuchi S, and Nakamura-Uchiyama F
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- Male, Adult, Female, Humans, Middle Aged, Japan, SARS-CoV-2, Capillary Leak Syndrome complications, Capillary Leak Syndrome diagnosis, Capillary Leak Syndrome therapy, COVID-19 complications, COVID-19 diagnosis, Shock complications, Shock diagnosis
- Abstract
Systemic Capillary Leak Syndrome (SCLS) is a rare disease that causes severe distributive shock provoked by infection or vaccination. SCLS is clinically diagnosed by a triad of distributive shock, paradoxical hemoconcentration, and hypoalbuminemia. SCLS associated with coronavirus disease (COVID-19) in adults has not been reported yet in Japan. Case 1: A 61-year-old woman with fever, sore throat, headache, and muscle pain was admitted to our emergency department with suspected COVID-19. She had been diagnosed with SCLS 3 years earlier. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen and polymerase chain reaction (PCR) tests were negative at admission. She went into shock in the emergency department and was treated for septic shock. The following day, the SARS-CoV-2 PCR test was positive. She did not respond to fluid resuscitation and catecholamine and finally died. Case 2: A 58-year-old man was admitted to our hospital for de-saturation due to COVID-19. He got into shock on day 3. SCLS was suspected, and 5 g of intravenous immunoglobulin and 5% albumin were administered for sepsis treatment. He responded to the aggressive fluid therapy within 48 h and was finally discharged. COVID-19 can trigger SCLS, and early recognition of SCLS is crucial for survival. Primary care physicians should consider SCLS when they observe distributive shock and paradoxical hemoconcentration deviations from the natural course of COVID-19., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Infection Prevention and Control. Published by Elsevier Ltd. All rights reserved.)
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- 2024
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6. Non-serogroupable Neisseria meningitidis pneumonia in an immunocompetent patient with severe COVID-19 pneumonia: A case report.
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Kojima H, Nakamura-Uchiyama F, Ariyoshi T, Kosaka A, Washino T, Sakamoto N, Iwabuchi S, and Makino J
- Abstract
Background: Non-serogroupable Neisseria meningitidis ( N. meningitidis ) , the most common type of N. meningitidis in asymptomatic carriers, rarely causes infections. Most reported cases of infection are in patients with immunodeficiency, primarily complement deficiencies., Case Presentation: A 54-year-old immunocompetent man was transferred to our hospital to treat severe coronavirus disease 2019 (COVID-19). The patient presented with cough producing a large amount of purulent sputum, which was considered an atypical presentation of COVID-19. Gram staining of the sputum revealed a large number of gram-negative diplococci phagocytosed by many neutrophils, and a diagnosis of bacterial pneumonia was established. The culture yielded non-serogroupable N. meningitidis, and the patient was diagnosed with non-serogroupable N. meningitidis pneumonia. Potential immunodeficiency was considered; however, testing including human immunodeficiency virus and complement factors showed no abnormalities., Conclusions: We report herein a rare case of non-serogroupable N. meningitidis pneumonia that occurred in an immunocompetent patient during the course of severe COVID-19. We consider impaired T cell function attributable to COVID-19 and dexamethasone administration may have triggered a transient immunosuppressive state and led to non-serogroupable N. meningitidis pneumonia., Competing Interests: None declared., (© 2022 The Authors.)
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- 2022
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7. Disseminated Toxoplasmosis associated with Haemophagocytic Lymphohistiocytosis in a Patient with the Human Immunodeficiency Virus: A Case Report and Literature Review.
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Washino T, Mikita K, Kosaka A, Sakamoto N, Iwabuchi S, and Nakamura-Uchiyama F
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- Adult, Clindamycin therapeutic use, Female, HIV, Humans, Sulfamethoxazole therapeutic use, Trimethoprim therapeutic use, HIV Infections complications, Lymphohistiocytosis, Hemophagocytic complications, Lymphohistiocytosis, Hemophagocytic diagnosis, Lymphohistiocytosis, Hemophagocytic drug therapy, Toxoplasmosis complications, Toxoplasmosis diagnosis, Toxoplasmosis drug therapy
- Abstract
Disseminated toxoplasmosis associated with haemophagocytic lymphohistiocytosis (DT-HLH) is rare and difficult to diagnose compared to disseminated toxoplasmosis or HLH presenting alone. Because of the limited number of reported cases, the clinical characteristics and outcomes of DT-HLH are unknown. We report a case of DT-HLH in a human immunodeficiency virus (HIV)-infected patient who was successfully treated with early anti-toxoplasmic therapy and performed a comprehensive literature review. A 33-year-old Cameroonian woman was transferred to our hospital owing to HIV infection and encephalitis. Although she developed HLH, bone marrow biopsy did not reveal the cause. She was diagnosed as having DT-HLH via polymerase chain reaction testing of bone marrow biopsy tissue, blood, and cerebrospinal fluid. DT-HLH improved within the initial two weeks of treatment for toxoplasmosis (sulfamethoxazole-trimethoprim, trimethoprim 10 mg/kg/day and clindamycin 1,800 mg/day) before the introduction of antiretroviral therapy. To our knowledge, only eight cases of DT-HLH have been previously reported in the literature. Most patients died within three weeks of hospitalisation and were diagnosed by autopsy. Conversely, patients diagnosed antemortem were all treated and survived, including the currently reported patient. DT-HLH can lead to poor prognosis without early and proper treatment. Clinicians should consider toxoplasmosis in the differential diagnosis of HLH., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2022
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8. COVID-19-associated leukoencephalopathy in the absence of severe hypoxia with subsequent improvement: a case report.
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Kojima H, Sakamoto N, Kosaka A, Kobayashi M, Amemiya M, Washino T, Kuwahara Y, Ishida T, Hikone M, Miike S, Oyabu T, Iwabuchi S, and Nakamura-Uchiyama F
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- Aged, COVID-19 Testing, Female, Humans, Hypoxia etiology, SARS-CoV-2, COVID-19 complications, COVID-19 diagnosis, Leukoencephalopathies diagnosis
- Abstract
Background: Several cases of coronavirus disease 2019 (COVID-19)-associated leukoencephalopathy have been reported. Although most cases involve hypoxia, the pathophysiological mechanism and neurologic outcomes of COVID-19-associated leukoencephalopathy remain unclear., Case Presentation: We report a case of COVID-19-associated leukoencephalopathy without severe hypoxia in a 65-year-old woman diagnosed with pyelonephritis. After the initiation of intravenous ceftriaxone, her fever resolved, but she developed an altered state of consciousness with abnormal behavior and, subsequently, a relapse fever. She was diagnosed with COVID-19 pneumonia and was intubated. Lung-protective ventilation with deep sedation and neuromuscular blockade were used for treatment. After cessation of sedative administration, her mental status remained at a Glasgow Coma Scale score of 3. COVID-19 was assumed to have caused leukoencephalopathy due to the absence of severe hypoxia or other potential causes. She subsequently showed gradual neurologic improvement. Three months after the COVID-19 diagnosis, she regained alertness, with a Glasgow Coma Scale score of 15., Conclusion: Clinicians should consider leukoencephalopathy in the differential diagnosis of consciousness disorders in patients with severe COVID-19, even in the absence of severe hypoxia. Gradual neurologic improvement can be expected in such cases., (© 2022. The Author(s).)
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- 2022
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9. Nosocomial outbreak of coronavirus disease in two general wards during the initial wave of the pandemic in 2020, Tokyo, Japan.
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Sakamoto N, Ota M, Takeda T, Kosaka A, Washino T, Iwabuchi S, Beppu M, Nishiduka I, Matsui T, Suzuki M, and Nakamura-Uchiyama F
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- Disease Outbreaks prevention & control, Hospitals, Humans, Japan epidemiology, Pandemics prevention & control, Patients' Rooms, SARS-CoV-2, Tokyo epidemiology, COVID-19 epidemiology, COVID-19 prevention & control, Cross Infection epidemiology, Cross Infection prevention & control
- Abstract
Objective: Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was first reported in China and subsequently spread worldwide. In Japan, many clusters occurred during the first wave in 2020. We describe the investigation of an early outbreak in a Tokyo hospital., Methods: A COVID-19 outbreak occurred in two wards of the hospital from April to early May 2020. Confirmed cases were individuals with laboratory-confirmed SARS-CoV-2 infection linked to Wards A and B, and contacts were patients or workers in Wards A or B 2 weeks before the index cases developed symptoms. All contacts were tested, and cases were interviewed to determine the likely route of infection and inform the development of countermeasures to curb transmission., Results: There were 518 contacts, comprising 472 health-care workers (HCWs) and 46 patients, of whom 517 were tested. SARS-CoV-2 infection was confirmed in 42 individuals (30 HCWs and 12 patients). The proportions of SARS-CoV-2 infections in HCWs were highest among surgeons, nurses, nursing assistants and medical assistants. Several HCWs in these groups reported being in close proximity to one another while not wearing medical masks. Among HCWs, infection was thought to be associated with the use of a small break room and conference room., Discussion: Nosocomial SARS-CoV-2 infections occurred in two wards of a Tokyo hospital, affecting HCWs and patients. Not wearing masks was considered a key risk factor for infection during this outbreak; masks are now a mandated countermeasure to prevent the spread of SARS-CoV-2 infection in hospital settings., ((c) 2022 The authors; licensee World Health Organization.)
- Published
- 2022
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10. Separation of microalgae using a compacted magnetite-containing gel bed.
- Author
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Washino T, Demura M, Morisada S, Ohto K, and Kawakita H
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- Ferrosoferric Oxide, Water, Microalgae, Stramenopiles
- Abstract
Separation of microalgae of various sizes and shapes is an important process that enables subsequent production of useful compounds. Herein, the separation of microalgae was accomplished using a magnetite-containing gel (42 μm) packed into a column. An algal suspension was injected into the top of the gel bed, after which water was passed through the column. The pressure generated during the process caused the lower domain of the gel bed to deform, resulting in narrowed gaps between the gel beads. When a suspension of Nannochloropsis sp. (0.0069-0.69 g L
-1 ) was loaded and water was passed through the column at an applied pressure of 0.01-0.10 MPa, the majority of microalgae were captured within the upper domain of the gel bed, while only 20% were captured within the lower domain. The amount of Nannochloropsis sp. captured was expressed by an ordinary differential equation to determine the capture coefficient, K, and the maximum capture amount, Qmax . As pressure increased, gel gaps narrowed, K increased, and Qmax decreased because of a reduction in the number of effective capture sites upon compaction of the gel. When a mixed suspension of Anabaena sp., Monoraphidium sp., and Desmodesmus sp. (0.069 g L-1 each) was injected into the gel bed at an applied pressure of 0.01 MPa, only Anabaena sp. was captured at the bottom of the gel bed. This device can be applied for the separation of microalgae in rivers and the sea., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2022
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11. Current antimicrobial prescription at outpatient dentistry centers and clinics in tertiary-care hospitals in Tokyo, Japan: A multicenter cross-sectional study.
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Tagashira Y, Horiuchi M, Kosaka A, Washino T, Horiuchi M, Murakami S, Tagashira I, and Honda H
- Abstract
Background: Antimicrobial administration is necessary before specific dental procedures to prevent postprocedural infections and complications and antimicrobials are sometimes indicated for the treatment of odontogenic infections. However, antimicrobials are commonly misused by dentists., Methods: This cross-sectional study was conducted at 4 public, tertiary-care hospitals in Tokyo, Japan, from June to July 2019. We included patients who received an antimicrobial prescription at the outpatient dentistry center or clinic at each participating hospital. The indications for antimicrobial prescription were (1) antimicrobial prescriptions for prophylaxis (APPs) or (2) antimicrobial prescriptions for treatment (APTs). Prescribing patterns were described in terms of antimicrobial choice, timing, and dosages for APPs and APTs., Results: During the study period, 1,772 patients received an antimicrobial prescription. Among them 1,439 (81.2%) were APPs and 333 (18.8%) were APTs. The most common aim of APP was to prevent local infections and complications following tooth extraction (n = 1,244, 86.4%). The proportion of appropriate APPs was only 0.8% (12 of 1,439). Among 1,439 total APPs, 171 (11.9%) were unnecessary, 32 (2.2%) were inappropriate, and 1,224 (85.1%) were suboptimal. Whereas 101 (30.3%) of 333 APTs were appropriate, the remaining 97 instances (29.1%) were unnecessary, 86 (26.7%) were inappropriate, and 46 (13.8%) were suboptimal., Conclusion: Inappropriate antimicrobial prescriptions were common among dentists in Japan. Understanding the differences in the current antimicrobial prescribing patterns for prophylaxis and treatment is critically important for implementing an effective antimicrobial stewardship program in dentistry., (© The Author(s) 2021.)
- Published
- 2021
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12. Epidemiological trends of imported infectious diseases in Japan: Analysis of imported 2-year infectious disease registry data.
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Kutsuna S, Asai Y, Yamamoto K, Shirano M, Konishi K, Asaoka T, Yamato M, Katsuragi Y, Yamamoto Y, Sahara T, Tamiya A, Nakamura-Uchiyama F, Sakamoto N, Kosaka A, Washino T, Hase R, Mito H, Kurita T, Shinohara K, Shimizu T, Kodama F, Nagasaka A, Ogawa T, Kasahara K, Yoshimura Y, Tachikawa N, Yokota K, Yuka Murai NS, Sakamaki I, Hasegawa C, Yoshimi Y, Toyoda K, Mitsuhashi T, and Ohmagari N
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- Animals, Asia, Diarrhea, Europe, Humans, Japan epidemiology, North America, Registries, Travel, Communicable Diseases epidemiology, Communicable Diseases, Imported diagnosis, Communicable Diseases, Imported epidemiology
- Abstract
Introduction: The epidemiology of infectious diseases in Japan remains undefined despite the increasing tourism. GeoSentinel, an epidemiological surveillance system for reporting imported infectious diseases, has only two participating facilities in Japan. Although the number of infectious diseases is reported by the National Institute of Infectious Diseases, there is no detailed clinical information about these cases. Therefore, we established J-RIDA (Japan Registry for Infectious Diseases from Abroad) to clarify the status of imported infectious diseases in Japan and provide detailed information., Methods: J-RIDA was started as a registry of imported infectious diseases. Case registration began in October 2017. Between October 2017 and September 2019, 15 medical institutions participated in this clinical study. The registry collected information about the patient's age, sex, nationality, chief complaint, consultation date, date of onset, whether visit was made to a travel clinic before travel, blood test results (if samples were collected), travel history, and final diagnosis., Results: Of the 3046 cases included in this study, 46.7% to Southeast Asia, 13.0% to Africa, 13.7% to East Asia, 11.5% to South Asia, 7.5% to Europe, 3.8% to Central and South America, 4.6% to North America, 3.9% to Oceania, and 2.8% to Central and west Asia. More than 85% of chief complaints were fever and general symptoms, gastrointestinal symptoms, respiratory symptoms, or dermatologic problems. The most common diseases were travelers' diarrhea, animal bite, upper respiratory infection, influenza, and dengue fever., Conclusions: We summarized two-year cases registered in Japan's imported infectious disease registry. These results will significantly contribute to the epidemiology in Japan., Competing Interests: Declaration of competing interest None declared., (Copyright © 2020 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2021
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13. Critically ill patients with COVID-19 in Tokyo, Japan: A single-center case series.
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Miike S, Sakamoto N, Washino T, Kosaka A, Kuwahara Y, Ishida T, Hikone M, Oyabu T, Kojima H, Iwabuchi S, and Nakamura-Uchiyama F
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- Adult, Aged, Aged, 80 and over, COVID-19 mortality, COVID-19 therapy, Comorbidity, Critical Illness mortality, Critical Illness therapy, Diabetes Mellitus epidemiology, Extracorporeal Membrane Oxygenation, Female, Humans, Hypertension epidemiology, Japan, Male, Middle Aged, Obesity epidemiology, Overweight epidemiology, Respiratory Insufficiency epidemiology, Retrospective Studies, SARS-CoV-2, Tokyo, Treatment Outcome, COVID-19 epidemiology, Critical Illness epidemiology, Respiration, Artificial methods
- Abstract
Introduction: We reported, in our previous study, a patient with coronavirus disease 2019 (COVID-19) who was successfully treated with extracorporeal membrane oxygenation. Data on clinical courses and outcomes of critically ill patients with COVID-19 in Japan are limited in the literature. This study aimed to describe the clinical courses and outcomes of critically ill patients with COVID-19 in Tokyo, Japan., Methods: This is a single-center case series study. Patients with COVID-19 treated with mechanical ventilation (MV) were reviewed retrospectively. Data on baseline characteristics, in-hospital treatment, and outcomes were collected., Results: Between February 2, 2020, and June 30, 2020, 14 critically ill patients with COVID-19 were treated with MV. Most patients were male and had comorbidities, especially hypertension or diabetes; 35.7% were overweight and 21.4% were obese. The majority of the patients had dyspnea on admission. The median duration of MV was 10.5 days, and the 28-day mortality rate was 35.7%. In the four patients with COVID-19 who died, the cause of death was respiratory failure., Conclusions: As in previous reports from other countries, the mortality rate of patients with COVID-19 requiring intensive care remains high in Tokyo. Further study on the appropriate timing of MV initiation and specific treatments for critically ill patients with COVID-19 is needed., Competing Interests: Declaration of competing interest None., (Copyright © 2020 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2021
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14. A sporadic COVID-19 pneumonia treated with extracorporeal membrane oxygenation in Tokyo, Japan: A case report.
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Nakamura K, Hikone M, Shimizu H, Kuwahara Y, Tanabe M, Kobayashi M, Ishida T, Sugiyama K, Washino T, Sakamoto N, and Hamabe Y
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- Antiviral Agents therapeutic use, Betacoronavirus, COVID-19, Critical Care, Drug Combinations, Humans, Infection Control, Japan, Lopinavir therapeutic use, Male, Middle Aged, Pandemics, Pneumonia, Viral complications, Pneumonia, Viral virology, Respiration, Artificial, Ritonavir therapeutic use, SARS-CoV-2, Coronavirus Infections complications, Coronavirus Infections therapy, Extracorporeal Membrane Oxygenation, Pneumonia, Viral therapy
- Abstract
Introduction: An ongoing outbreak of a novel coronavirus disease (coronavirus disease 2019, COVID-19) has become a global threat. While clinical reports from China to date demonstrate that the majority of cases remain relatively mild and recover with supportive care, it is also crucial to be well prepared for severe cases warranting intensive care. Initiating appropriate infection control measures may not always be achievable in primary care or in acute-care settings., Case: A 45-year-old man was admitted to the intensive care unit due to severe pneumonia, later confirmed as COVID-19. His initial evaluation in the resuscitation room and treatments in the intensive care unit was performed under droplet and contact precaution with additional airborne protection using the N95 respirator mask. He was successfully treated in the intensive care unit with mechanical ventilation and extracorporeal membrane oxygenation for respiratory support; and antiretroviral treatment with lopinavir/ritonavir. His total intensive care unit stay was 15 days and was discharged on hospital day 24., Conclusions: Strict infection control precautions are not always an easy task, especially under urgent care in an intensive care unit. However, severe cases of COVID-19 pneumonia, or another novel infectious disease, could present at any moment and would be a continuing challenge to pursue appropriate measures. We need to be well prepared to secure healthcare workers from exposure to infectious diseases and nosocomial spread, as well as to provide necessary intensive care., Competing Interests: Declaration of Competing Interest None., (Copyright © 2020 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2020
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15. Failure of Liposomal-amphotericin B Treatment for New World Cutaneous Leishmaniasis due to Leishmania braziliensis.
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Kosaka A, Sakamoto N, Hikone M, Imai K, Ota M, Washino T, Maeda T, and Iwabuchi S
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- Amphotericin B administration & dosage, Antiprotozoal Agents administration & dosage, Diagnosis, Differential, Dose-Response Relationship, Drug, Humans, Leishmaniasis, Cutaneous complications, Leishmaniasis, Cutaneous drug therapy, Male, Middle Aged, Retreatment, Treatment Failure, Amphotericin B therapeutic use, Antiprotozoal Agents therapeutic use, Leishmania braziliensis isolation & purification, Leishmaniasis, Cutaneous diagnosis
- Abstract
Liposomal-amphotericin B (L-AmB) is used for cutaneous leishmaniasis (CL); however, its treatment failure has not yet been described in detail. A 58-year-old man returned from the Republic of Venezuela with a cutaneous ulcer on his left lower leg. The causative pathogen was Leishmania braziliensis. We started L-AmB 3 mg/kg/day for 6 days; however, the ulcer did not resolve. The patient was successfully retreated with a higher dose L-AmB 4 mg/kg/day 9 times (total, 36 mg/kg). If L-AmB fails to treat CL and other therapeutics cannot be used, increasing the L-AmB dose is a viable option.
- Published
- 2020
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16. The first case report of infective endocarditis caused by Gemella taiwanensis.
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Hikone M, Sakamoto N, Ota M, Washino T, Kobayashi KI, Iwabuchi S, Kazama H, Kounosu A, Negishi K, Ainoda Y, Iguchi S, Yoshida A, Kikuchi K, and Ohnishi K
- Subjects
- Anti-Bacterial Agents therapeutic use, Bacterial Proteins genetics, Chaperonin 60 genetics, DNA, Bacterial analysis, DNA, Bacterial genetics, Female, Humans, Middle Aged, Phylogeny, RNA, Ribosomal, 16S genetics, Endocarditis, Bacterial diagnosis, Endocarditis, Bacterial drug therapy, Endocarditis, Bacterial microbiology, Gemella classification, Gemella genetics, Gram-Positive Bacterial Infections diagnosis, Gram-Positive Bacterial Infections drug therapy, Gram-Positive Bacterial Infections microbiology
- Abstract
Gemella is a facultative anaerobic Gram-positive coccus and a rare cause of infective endocarditis (IE). Gram staining may eventually misidentify the organism, which tends to easily decolorize and manifest as either Gram-negative or Gram-variable. Commercial biochemical tests are often used to identify Gemella, but the methods they employ sometimes lack accuracy. A 52-year-old woman was diagnosed with Gemella taiwanensis IE after initial identification of the pathogen as Gemella haemolysans using biochemical tests combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). She was treated successfully with penicillin, gentamicin, and mitral valve replacement. To our knowledge, this is the first case of IE confirmed by 16S rRNA gene and groEL sequencing to have been caused by G. taiwanensis. The accurate diagnosis of rare or difficult-to-identify pathogens is a major challenge for clinical microbiological laboratories. The concurrent use of molecular methods could lead to the recognition of new or different pathogens., (Copyright © 2017 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2017
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17. Streptococcal toxic shock syndrome secondary to group A Streptococcus vaginitis.
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Hikone M, Kobayashi K, Washino T, Ota M, Sakamoto N, Iwabuchi S, and Ohnishi K
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- Female, Humans, Intraabdominal Infections diagnosis, Intraabdominal Infections drug therapy, Intraabdominal Infections microbiology, Middle Aged, Shock, Septic drug therapy, Staphylococcal Infections drug therapy, Streptococcus pyogenes drug effects, Vaginosis, Bacterial drug therapy, Shock, Septic diagnosis, Shock, Septic microbiology, Staphylococcal Infections diagnosis, Streptococcus pyogenes isolation & purification, Vaginosis, Bacterial diagnosis
- Abstract
Streptococcal toxic shock syndrome (TSS) is a systemic illness usually caused in the setting of infection by group A Streptococcus (GAS). The primary infections are often invasive infections of the respiratory tract or necrotizing infections of the skin and soft tissue, but some infections occur without relevant focus. GAS vaginitis is a rare condition among adult women and is accordingly thought to be uncommon as a cause of streptococcal TSS. Here we report the cases of two postmenopausal women with streptococcal TSS secondary to GAS vaginitis, one aged 55 and one aged 60. Both came to our emergency department with complaints or symptoms of abdominal pain, fever, hypotension, and multi-organ failure. In both cases, the relevant factor associated with streptococcal infection was a recent episode of GAS vaginitis. Both underwent fluid management and 14 days of antibiotic treatment and fully recovered without complications. Vaginitis was likely to be the primary infectious trigger of TSS in these two cases. Intrauterine device insertion, endometrial biopsy, and post-partum state have all been previously reported in TSS patients, and the female genital tract has been described as a portal of entry. GAS vaginitis warrants appropriate treatment as it may progress to severe systemic infection as described., (Copyright © 2015 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2015
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18. Anterior mediastinal abscess diagnosed in a young sumo wrestler after closed blunt chest trauma.
- Author
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Sassa T, Kobayashi K, Ota M, Washino T, Hikone M, Sakamoto N, Iwabuchi S, Otsuji M, and Ohnishi K
- Subjects
- Abscess diagnosis, Adolescent, Anti-Bacterial Agents therapeutic use, Combined Modality Therapy, Debridement, Diagnosis, Differential, Drainage, Humans, Magnetic Resonance Imaging, Male, Mediastinal Diseases diagnosis, Staphylococcal Infections diagnosis, Thoracic Injuries diagnosis, Tomography, X-Ray Computed, Wounds, Nonpenetrating diagnosis, Abscess microbiology, Abscess therapy, Mediastinal Diseases microbiology, Mediastinal Diseases therapy, Staphylococcal Infections microbiology, Staphylococcal Infections therapy, Thoracic Injuries microbiology, Thoracic Injuries therapy, Wounds, Nonpenetrating microbiology, Wounds, Nonpenetrating therapy, Wrestling injuries
- Abstract
Most mediastinal abscesses result from infections after thoracotomy, esophageal perforation or pene- trating chest trauma. This disease is rarely caused by closed blunt chest trauma. All previously reported such cases after closed blunt chest trauma presented with hematoma and sternal osteomyelitis resulting from sternal fracture. Here we report a 15-year-old sumo wrestler who presented with an anterior mediastinal abscess without any mediastinal fracture. The mediastinal abscess resulted from the hematogenous spread of Staphylococcus aureus to a hematoma that might have been caused by a closed blunt chest trauma incurred during sumo wrestling exercises.
- Published
- 2015
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19. Toxoplasma gondii infection inhibits Th17-mediated spontaneous development of arthritis in interleukin-1 receptor antagonist-deficient mice.
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Washino T, Moroda M, Iwakura Y, and Aosai F
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- Animals, CD11c Antigen biosynthesis, CD4-Positive T-Lymphocytes immunology, GATA3 Transcription Factor biosynthesis, GATA3 Transcription Factor genetics, Interferon-gamma biosynthesis, Interleukin 1 Receptor Antagonist Protein immunology, Interleukin-10 biosynthesis, Interleukin-12 Subunit p40 biosynthesis, Interleukin-12 Subunit p40 genetics, Interleukin-17 biosynthesis, Interleukin-17 genetics, Interleukin-1beta biosynthesis, Interleukin-4 biosynthesis, Interleukin-4 genetics, Interleukin-6 biosynthesis, Lymph Nodes pathology, Mice, Mice, Inbred BALB C, Mice, Knockout, Nuclear Receptor Subfamily 1, Group F, Member 3 biosynthesis, Nuclear Receptor Subfamily 1, Group F, Member 3 genetics, RNA, Messenger biosynthesis, Spleen cytology, Th1 Cells immunology, Th2 Cells immunology, Toxoplasmosis metabolism, Arthritis immunology, Hereditary Autoinflammatory Diseases immunology, Th17 Cells immunology, Toxoplasma, Toxoplasmosis immunology
- Abstract
Interleukin 1 receptor antagonist (IL-1Ra)-deficient BALB/c mice develop spontaneous arthritis resembling human rheumatoid arthritis. We herein report that infection with Toxoplasma gondii, an intracellular protozoan, is capable of ameliorating the spontaneous development of arthritis in IL-1Ra-deficient mice. The onset of arthritis development was delayed and the severity score of arthritis was significantly suppressed in T. gondii-infected mice. Expression of IL-12p40 mRNA from CD11c(+) cells of mesenteric lymph nodes (mLN) and spleen markedly increased at 1 week after peroral infection. While CD11c(+) cells also produced IL-10, IL-1β, and IL-6, CD4(+) T cells from T. gondii-infected mice expressed significantly high levels of T-bet and gamma interferon (IFN-γ) mRNA in both mLN and spleen. Levels of GATA-3/IL-4 mRNA or RORγt/IL-17 mRNA decreased in the infected mice, indicating Th1 cell polarization and the reduction of Th2 and Th17 cell polarization. The severity of arthritis was related to Th1 cell polarization accompanied by Th17 cell reduction, demonstrating the protective role of the T. gondii-derived Th1 response against Th17 cell-mediated arthritis in IL-1Ra-deficient mice.
- Published
- 2012
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20. Suppressed phenylalanine ammonia-lyase activity after heat shock in transgenic Nicotiana plumbaginifolia containing an Arabidopsis HSP18.2-parsley PAL2 chimera gene.
- Author
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Moriwaki M, Yamakawa T, Washino T, Kodama T, and Igarashi Y
- Abstract
The activity of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) after heat shock (HS) in leaves and buds of transgenic Nicotiana plumbaginifolia containing an Arabidopsis HSP18.2 promoter-parsley phenylalanine ammonia-lyase 2 (HSP18.2-PAL2) chimera gene was examined. Immediately after HS treatment at 44 degrees C for 5 h, the PAL activity in both transgenic and normal (untransformed) plants was 35-38% lower than that before HS. At normal temperature (25-26 degrees C), the PAL activity recovered within 5 h of ending the HS treatment in normal plants, but not until 12-24 h in transgenic plants containing the HSP18.2-PAL2 gene. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed the presence of parsley PAL2 mRNA in transgenic plants, which remained for 8-12 h following 5-h HS at 44 degrees C; the mRNA was not observed before HS. The content of chlorogenic acid (CGA; 3-caffeoylquinic acid) decreased drastically 8-12 h after HS in transgenic plants, but only slightly in normal plants. Thus, the decrease in PAL activity accompanied by expression of the parsley PAL2 gene after HS treatment corresponded to the decrease in CGA synthesis. These results might be attributed to post-transcriptional degradation of endogenous PAL mRNA triggered by transcription of the transgene.
- Published
- 1999
- Full Text
- View/download PDF
21. Purification and some properties of a trehalase from a green alga, Lobosphaera sp.
- Author
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Nakano H, Moriwaki M, Washino T, Kino T, Yoshizumi H, and Kitahata S
- Subjects
- Carbohydrate Metabolism, Carbohydrate Sequence, Chromatography, Affinity, Electrophoresis, Polyacrylamide Gel, Hydrogen-Ion Concentration, Metals pharmacology, Microscopy, Electron, Molecular Sequence Data, Molecular Weight, Substrate Specificity, Temperature, Trehalase chemistry, Trehalase metabolism, Chlorophyta enzymology, Trehalase isolation & purification
- Abstract
An unicellular green alga identified as Lobosphaera sp. by morphological observations was selected as a source of trehalase. The alga grew well heterotrophically and produced intracellular trehalase using Polypepton, yeast extract, and glycerol as nutrients. The enzyme was highly purified by ammonium sulfate fractionation, column chromatography on DEAE-Toyopearl, Sepharose CL-4B, and SP-Toyopearl. The molecular mass was estimated to be 400 kDa by gel filtration. SDS-PAGE indicated that the enzyme consisted of two subunits with a molecular mass range of 180-220 kDa and it contained carbohydrates. The enzyme was most active at pH 5.5 and at 65 degrees C and stable between pH 4-9 and below 65 degrees C. Fe3+ inactivated the enzyme. Sucrose was a competitive inhibitor with a Ki of 7.5 mM. The enzyme specifically hydrolyzed trehalase with a Km of 0.6 mM.
- Published
- 1994
- Full Text
- View/download PDF
22. [Distribution, metabolism and excretion of 14C-MT-141 in rats. I. Distribution, metabolism and excretion after single intravenous administration in male rats].
- Author
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Komiya I, Ishizuka T, Nishio M, Murata S, Esumi Y, Washino T, Matsunaga K, Mitsugi K, and Yokoshima T
- Subjects
- Animals, Autoradiography, Carbon Radioisotopes, Cephalosporins administration & dosage, Cephamycins administration & dosage, Half-Life, Injections, Intravenous, Male, Rats, Rats, Inbred Strains, Tissue Distribution, Cephalosporins metabolism, Cephamycins metabolism
- Abstract
The distribution, metabolism and excretion of the radioactivity were studied in male rats after the bolus intravenous administration of 14C-MT-141. The biological half-lives obtained from the blood concentration-time curve were 0.43 hour for the data in the first 4 hours and 16.5 hours for the data from 6 hours to 24 hours after the intravenous administration of 14C-MT-141. The radioactivity was excreted mainly into urine, and the cumulative urinary and fecal excretion of the radioactivity were 75.2% and 24.1% of the dose, respectively, within 120 hours after the intravenous administration of 14C-MT-141. The cumulative biliary excretion of the radioactivity was 18% of the dose within 48 hours after the intravenous administration, and 35% of the radioactivity excreted into bile (about 6% of the dose) was reabsorbed from the intestine. The highest concentration of the radioactivity was observed in the kidneys, and also the relatively high concentrations were observed in the liver, plasma and intestine, while the concentrations in the brain, fat and muscle were low. Within 24 hours after the intravenous administration of 14C-MT-141, the radioactivity in the highly distributed organs or tissues was decreased to less than 3% of the values at 5 minutes after the intravenous administration. A small amount of N-acetyl-MT-141 was found in urine and feces as a metabolite.
- Published
- 1984
23. [Distribution, metabolism and excretion of 14C-MT-141 in rats. II. Distribution and excretion after multiple intravenous administration in male rats and after single intravenous administration in female rats].
- Author
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Komiya I, Ishizuka T, Nishio M, Murata S, Esumi Y, Washino T, Matsunaga K, Takaichi M, and Yokoshima T
- Subjects
- Animals, Autoradiography, Cephalosporins administration & dosage, Cephamycins administration & dosage, Female, Fetus metabolism, Injections, Intravenous, Male, Maternal-Fetal Exchange, Milk metabolism, Pregnancy, Rats, Tissue Distribution, Cephalosporins metabolism, Cephamycins metabolism
- Abstract
The distribution and tissue accumulation of the radioactivity were studied in male rats after the multiple intravenous administration of 14C-MT-141. The distribution and the placental transfer were also studied using pregnant rats or lactating rats after the single intravenous administration of 14C-MT-141. The radioactive concentration in the fetus was low and the radioactivity was distributed almost uniformly through the fetus body. The peak time of the milk level was 2 hours after the administration and the radioactivity in milk decreased gradually thereafter. The milk levels decreased more slowly than the blood levels did. The blood level after the last dose administered daily for 7 days tended to decrease more slowly, when compared with the single administration. However the blood concentration at 48 hours after the last administration was less than 3 times as high as that after the single administration.
- Published
- 1984
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