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498 results on '"Ward, D. C."'

1. Metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes.

Author

Jiang, J, Gill, B S, Wang, G L, Ronald, P C, and Ward, D C

Abstract

Fluorescence in situ hybridization (FISH) is a powerful tool for physical mapping in human and other mammalian species. However, application of the FISH technique has been limited in plant species, especially for mapping single- or low-copy DNA sequences, due to inconsistent signal production in plant chromosome preparations. Here we demonstrate that bacterial artificial chromosome (BAC) clones can be mapped readily on rice (Oryza sativa L.) chromosomes by FISH. Repetitive DNA sequences in BAC clones can be suppressed efficiently by using rice genomic DNA as a competitor in the hybridization mixture. BAC clones as small as 40 kb were successfully mapped. To demonstrate the application of the FISH technique in physical mapping of plant genomes, both anonymous BAC clones and clones closely linked to a rice bacterial blight-resistance locus, Xa21, were chosen for analysis. The physical location of Xa21 and the relationships among the linked clones were established, thus demonstrating the utility of FISH in plant genome analysis.

Published
1995

3. In Situ Formats

Author

Genersch, Elke, Heiles, B. J., Neumann, R., Herrington, C. Simon, Heino, Pirkko, Hukkanen, Veijo, Arnold, N., Bhatt, M., Ried, T., Wienberg, J., Ward, D. C., Koch, J., Raap, Anton K., Wiegant, Joop, Lichter, Peter, Schmidt, Erwin R., Starzinski-Powitz, Anna, Zimmermann, Katrin, Hillan, Kenneth J., Baldino, Frank, Jr., Robbins, Elaine, Lewis, Michael E., Tautz, Diethard, Patel, Nipam H., Goodman, Corey S., Cohen, Barbara, Cohen, Stephen M., and Kessler, Christoph, editor

Published
1992
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18. Irish paediatric association and welsh paediatric society: Proceedings of Joint Clinical Meeting, 17th–19th May, 1990 at Ardee Hotel, Waterford

Author

Brennan, A., Byrne, M., Gorby, A., Hoey, H., Alfaham, A., Goodchild, M. C., Campbell, I. A., Newcombe, R., Philpot, C., Fifield, R., Edwards, J., Conlon, T., Griffin, E., Clarke, T., Hilary, I., O’Connor, A., Walsh, J., Glasgow, J. F. T., Robinson, P. H., Moore, R., Crane, J., McKiernan, P., Fox, Gay, Gormally, Siobhan, Blakemore, Laurel, Matthews, T., MacMahon, P., Blair, M. E., Treweeke, I. Z., Kovar, Kemp, Alison, Sibert, Jo, Kemp, Alison, Sibert, Jo, Naughton, E., Gill, D., Hensey, O., Cahalane, S., Murphy, D., Pierce, A., Watson, J. B. C., McKenna, C., Flynn, A., Morrissey, P. A., Sweetnam, A., O’Haloran, E. T., Read, M., Owen, G., Dawson, S., Madarikn, B. A., Rees, B. I., Goodchild, M. C., Lynch, T., McMenamin, J., Wallace, S. J., Dowding, V. M., Barry, C., Earley, M. J., Fitzgerald, R., Philips, J., Garvey, M., Donoghue, V. B., Gorman, W. A., O’Brien, N., Murphy, J. F. A., Reardon, W., Genet, S., Middleton-Price, H., Feighery, C., Rowland, P., Jones, R. T., Doggah, M., Costigan, D. C., Leavy, P., Breathnach, F., Hensey, O., Fitzpatrick, C., Keenan, P., Corbally, M. T., Ryan, P., Nanshanie, A., Fitzpatrick, J., Fitzgerald, R. J., Dohil, R., Jones, V., Jenkins, H., Roberts, E., Lee, P. J., Jones, V., Kurien, A., O’Donoghue, E., Ward, O. C., Denham, B., Duff, D., Rao, S. J., Aburawi, E. H., Denham, B., Aburawi, E. H., Ward, D. C., and Denham, B.

Published
1991
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23. Actinomycin

Author

Reich, E., Cerami, A., Ward, D. C., Gottlieb, David, editor, and Shaw, Paul D., editor

Published
1967
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28. Congenital fibrosis of the extraocular muscles (autosomal dominant congenital external ophthalmoplegia): genetic homogeneity, linkage refinement, and physical mapping on chromosome 12

Author

Engle, E C, Marondel, I, Houtman, W A, de Vries, B, Loewenstein, A, Lazar, M, Ward, D C, Kucherlapati, R, and Beggs, A H

Subjects
Male, Chromosomes, Human, Pair 12, Ophthalmoplegia, Genetic Linkage, Chromosome Mapping, Humans, Female, Lod Score, Fibrosis, Research Article, Pedigree
Abstract

Congenital fibrosis of the extraocular muscles (CFEOM) is an autosomal dominant syndrome of congenital external ophthalmoplegia and bilateral ptosis. We previously reported linkage of this disorder in two unrelated families to an 8-cM region near the centromere of human chromosome 12. We now present refinement of linkage in the original two families, linkage analysis of five additional families, and a physical map of the critical region for the CFEOM gene. In each of the seven families the disease gene is linked to the pericentromeric region of chromosome 12. D12S345, D12S59, D12S331, and D12S1048 do not recombine with the disease gene and have combined lod scores of 35.7, 35.6, 16.0, and 31.4, respectively. AFM136xf6 and AFMb320wd9 flank the CFEOM locus, defining a critical region of 3 cM spanning the centromere of chromosome 12. These data support the concept that this may be a genetically homogeneous disorder. We also describe the generation of a YAC contig encompassing the critical region of the CFEOM locus. This interval has been assigned cytogenetically to 12p11.2-q12 and spans the centromere of chromosome 12. These results provide the basis for further molecular analyses of the structure and organization of the CFEOM locus and will help in the identification of candidate genes.

Published
1995

29. Narrowing the position of the Treacher Collins syndrome locus to a small interval between three new microsatellite markers at 5q32-33.1

Author

Dixon, M J, Dixon, J, Houseal, T, Bhatt, M, Ward, D C, Klinger, K, and Landes, G M

Subjects
Male, Base Sequence, Genetic Linkage, Centromere, Molecular Sequence Data, Chromosome Mapping, DNA, Satellite, Cosmids, Polymerase Chain Reaction, Pedigree, Chromosomes, Human, Pair 5, Humans, Female, Child, In Situ Hybridization, Fluorescence, Mandibulofacial Dysostosis, Research Article
Abstract

Treacher Collins syndrome (TCOF1) is an autosomal dominant disorder of craniofacial development, the features of which include conductive hearing loss and cleft palate. The TCOF1 locus has been localized to chromosome 5q32-33.2. In the present study we have used the combined techniques of genetic linkage analysis and fluorescence in situ hybridization (FISH) to more accurately define the TCOF1 critical region. Cosmids IG90 and SPARC, which map to distal 5q, encompass two and one hypervariable microsatellite markers, respectively. The heterozygosity values of these three markers range from .72 to .81. Twenty-two unrelated TCOF1 families have been analyzed for linkage to these markers. There is strong evidence demonstrating linkage to all three markers, the strongest support for positive linkage being provided by haplotyping those markers at the locus encompassed by the cosmid IG90 (Zmax = 19.65; theta = .010). FISH to metaphase chromosomes and interphase nuclei established that IG90 lies centromeric to SPARC. This information combined with the data generated by genetic linkage analysis demonstrated that the TCOF1 locus is closely flanked proximally by IG90 and distally by SPARC.

Published
1993

36. Kzf1 - a novel KRAB zinc finger protein encoding gene expressed during rat spermatogenesis.

Author

Bellefroid, Eric, Sahin, Mustafa, Poncelet, D A, Riviere, Michèle, Bourguignon, Claudine, Martial, J.A., Morris, P L, Pieler, T., Szpirer, Claude, Ward, D C, Bellefroid, Eric, Sahin, Mustafa, Poncelet, D A, Riviere, Michèle, Bourguignon, Claudine, Martial, J.A., Morris, P L, Pieler, T., Szpirer, Claude, and Ward, D C

Abstract

Two novel KRAB (Krüppel associated box) type zinc finger protein encoding cDNAs, named Kzf1 and Kzf2 (Kzf for KRAB zinc finger), were identified by screening of a rat embryonic brain cDNA library with a human ZNF91 KRAB probe. Kzf1 and Kzf2 encode proteins with an amino-terminal KRAB domain and a carboxy-terminal zinc finger cluster containing 9 and 13 zinc finger units, respectively. While Kzf2 appears to be ubiquitously expressed, Kzf1 is preferentially expressed in the testis. Within the testis, Kzf1 mRNA is restricted to germ cells. The Kzf1 protein exhibits DNA binding activity and its KRAB domain can function as a repressor module in transcription. Using somatic cell hybrid analysis, the Kzf1 gene was mapped to chromosome 6., Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S., info:eu-repo/semantics/published

Published
1998

38. Emergence of the ZNF91 Krüppel-associated box-containing zinc finger gene family in the last common ancestor of anthropoidea.

Author

Bellefroid, Eric, Marine, Jean-Christophe, Matera, A G, Bourguignon, Claudine, Desai, T, Healy, K C, Bray-Ward, P, Martial, J.A., Ihle, J N, Ward, D C, Bellefroid, Eric, Marine, Jean-Christophe, Matera, A G, Bourguignon, Claudine, Desai, T, Healy, K C, Bray-Ward, P, Martial, J.A., Ihle, J N, and Ward, D C

Abstract

The ZNF91 gene family, a subset of the Krüppel-associated box (KRAB)-containing group of zinc finger genes, comprises more than 40 loci; most reside on human chromosome 19p12-p13.1. We have examined the emergence and evolutionary conservation of the ZNF91 family. ZNF91 family members were detected in all species of great apes, gibbons, Old World monkeys, and New World monkeys examined but were not found in prosimians or rodents. In each species containing the ZNF91 family, the genes were clustered at one major site, on the chromosome(s) syntenic to human chromosome 19. To identify a putative "founder" gene, > 20 murine KRAB-containing zinc finger protein (ZFP) cDNAs were randomly cloned, but none showed sequence similarity to the ZNF91 genes. These observations suggest that the ZNF91 gene cluster is a derived character specific to Anthropoidea, resulting from a duplication and amplification event some 55 million years ago in the common ancestor of simians. Although the ZNF91 gene cluster is present in all simian species, the sequences of the human ZNF91 gene that confer DNA-binding specificity were conserved only in great apes, suggesting that there is not a high selective pressure to maintain the DNA targets of these proteins during evolution., Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S., info:eu-repo/semantics/published

Published
1995

39. Kinetoplast DNA replication: mechanistic differences between Trypanosoma brucei and Crithidia fasciculata.

Author

Ferguson, M L, Torri, A F, Perez-Morga, David, Ward, D C, Englund, P T, Ferguson, M L, Torri, A F, Perez-Morga, David, Ward, D C, and Englund, P T

Abstract

Kinetoplast DNA, the mitochondrial DNA of trypanosomatid parasites, is a network containing several thousand minicircles and a few dozen maxicircles. We compared kinetoplast DNA replication in Trypanosoma brucei and Crithidia fasciculata using fluorescence in situ hybridization and electron microscopy of isolated networks. One difference is in the location of maxicircles in situ. In C. fasciculata, maxicircles are concentrated in discrete foci embedded in the kinetoplast disk; during replication the foci increase in number but remain scattered throughout the disk. In contrast, T. brucei maxicircles generally fill the entire disk. Unlike those in C. fasciculata, T. brucei maxicircles become highly concentrated in the central region of the kinetoplast after replication; then during segregation they redistribute throughout the daughter kinetoplasts. T. brucei and C. fasciculata also differ in the pattern of attachment of newly synthesized minicircles to the network. In C. fasciculata it was known that minicircles are attached at two antipodal sites but subsequently are found uniformly distributed around the network periphery, possibly due to a relative movement of the kinetoplast disk and two protein complexes responsible for minicircle synthesis and attachment. In T. brucei, minicircles appear to be attached at two antipodal sites but then remain concentrated in these two regions. Therefore, the relative movement of the kinetoplast and the two protein complexes may not occur in T. brucei., Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
1994

40. Localization of the human KRAB finger gene ZNF117 (HPF9) to chromosome 7q11.2.

Author

Bellefroid, Eric, Ried, T, Riviere, Michèle, Marine, Jean-Christophe, Levan, G., Szpirer, Josiane, Szpirer, Claude, Ward, D C, Martial, J.A., Bellefroid, Eric, Ried, T, Riviere, Michèle, Marine, Jean-Christophe, Levan, G., Szpirer, Josiane, Szpirer, Claude, Ward, D C, and Martial, J.A.

Abstract

A cluster of Krüppel type zinc finger genes of the KRAB subclass has recently been localized on human chromosome 19p12-p13.1. We now report that ZNF117 (HPF9), a closely related zinc finger gene of this KRAB subfamily, has been assigned to a distinct locus in the human genome: chromosome band 7q11.2., Journal Article, Research Support, U.S. Gov't, P.H.S., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
1992

45. Internet-based Support for Bioscience Research: A Collaborative Genome center for Human Chromosome 12

Author

Miller, P. L., primary, Nadkarni, P. M., additional, Kidd, K. K., additional, Cheung, K., additional, Ward, D. C., additional, Banks, A., additional, Bray-Ward, P., additional, Cupelli, L., additional, Herdman, V., additional, Marondel, I., additional, Montcomery, K., additional, Renault, B., additional, Yoon, S.-J., additional, Krauter, K. S., additional, and Kucherlapati, R., additional

Published
1995
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