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This research evaluated the role and feasibility of the granular nanocellulose particles (GNC) from sugarcane bagasse obtained from enzymatic hydrolysis in reducing lipid digestibility and permeability in an in vitro simulated gastrointestinal (GI) system. GNC concentration (0.02%, w/v) had significantly affected the released free fatty acids (FFA), with a reduction of approximately 20%. Pickering emulsion of a GNC and olive oil simulation mixture revealed higher oil droplet size distribution and stability in the initial stage than the vortexed mixture formation. The difference in particle size distribution and zeta potential of the ingested GNC suspension and GNC–olive oil emulsion were displayed during the in vitro gastrointestinal simulation. GNC particles interacted and distributed surrounding the oil droplet, leading to interfacial emulsion. The GNC concentration (0.01–0.10%, w/v) showed low toxicity on HIEC-6 cells, ranging from 80.0 to 99% of cell viability. The release of FFA containing the ingested GNC suspension and GNC–olive oil emulsion had about a 30% reduction compared to that without the GNC digestion solution. The FFA and triglyceride permeability through the HIEC-6 intestinal epithelium monolayer were deceased in the digesta containing the ingested GNC and emulsion. This work indicated that GNC represented a significantly critical role and properties in the GI tract and reduced lipid digestion and absorption. This GNC could be utilized as an alternative food additive or supplement in fatty food for weight control due to their inhibition of lipid digestibility and assimilation.

An ethanolic glutinous black rice bran extract (RBE) was prepared and used in the experiments to determine the phenolic and flavonoid contents, antioxidation using DPPH and TBARS assay, anti-adipogenesis, and lipolysis induction in a 3T3-L1 pre-adipocyte model to discover its anti-obesity potential. The results showed that RBE was composed of phenolics, flavonoids, and anthocyanins and showed antioxidative activities (8.58±0.14 mg Vit C eq/g extract and 6.06±0.49 mg BHT eq/g extract). Interestingly, its anti-obesity potential was demonstrated in the interruption of the adipocyte life cycle, i.e. a significant anti-adipogenesis effect in the reduction of lipid accumulation (50.86±3.26 to 97.06±8.09% of the negative control, and triglyceride content in 3T3-L1 pre-adipocytes (36.89±2.08 to 90.56±0.67% of the negative control), and lipolysis induction (relative glycerol content 117.62±1.44 and 163.72±10.18% of control). Therefore, implementation into obesity therapy requires further research.