44 results on '"Wanting Niu"'
Search Results
2. Impairment of AMPK-α2 augments detrusor contractions in bladder ischemia
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Jing-Hua Yang, Wanting Niu, Yedan Li, and Kazem M. Azadzoi
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bladder ,ischemia ,metabolic stress ,overactive detrusor ,peripheral arterial disease ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Purpose: Ischemia disrupts cellular energy homeostasis. Adenosine monophosphate-activated protein kinase alpha-2 (AMPK-α2) is a subunit of AMPK that senses cellular energy deprivation and signals metabolic stress. Our goal was to examine the expression levels and functional role of AMPK-α2 in bladder ischemia. Materials and Methods: Iliac artery atherosclerosis and bladder ischemia were engendered in apolipoprotein E knockout rats by partial arterial endothelial denudation using a balloon catheter. After eight weeks, total and phosphorylated AMPK-α2 expression was analyzed by western blotting. Structural integrity of AMPK-α2 protein was assessed by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Functional role of AMPK-α2 was examined by treating animals with the AMPK activator 5-aminoimidazole-4-carboxamide-1-beta-D ribofuranoside (AICAR). Tissue contractility was measured in the organ bath and bladder nerve density was examined by immunostaining. Results: Total AMPK-α2 expression increased in bladder ischemia, while phosphorylated AMPK-α2 was significantly downregulated. LC-MS/MS suggested post-translational modification of AMPK-α2 functional domains including phosphorylation sites, suggesting accumulation of catalytically inactive AMPK-α2 in bladder ischemia. Treatment of rats with AICAR diminished the force of overactive detrusor contractions and increased bladder capacity but did not have a significant effect on the frequency of bladder contractions. AICAR diminished contractile reactivity of ischemic tissues in the organ bath and prevented loss of nerve fibers in bladder ischemia. Conclusions: Ischemia induces post-translational modification of AMPK-α2 protein. Impairment of AMPK-α2 may contribute to overactive detrusor contractions and loss of nerve fibers in bladder ischemia. AMPK activators may have therapeutic potential against detrusor overactivity and neurodegeneration in bladder conditions involving ischemia.
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- 2021
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3. Promoting Neuro‐Supportive Properties of Astrocytes with Epidermal Growth Factor Hydrogels
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Su Jing Chan, Wanting Niu, Kazuhide Hayakawa, Gen Hamanaka, Xiaoying Wang, Pike See Cheah, Shuzhen Guo, Zhangyang Yu, Ken Arai, Magdy H. Selim, Motoichi Kurisawa, Myron Spector, and Eng H. Lo
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Biomaterials ,Reactive astrocytes ,Neuroplasticity ,Stroke recovery ,Medicine (General) ,R5-920 ,Cytology ,QH573-671 - Abstract
Abstract Biomaterials provide novel platforms to deliver stem cell and growth factor therapies for central nervous system (CNS) repair. The majority of these approaches have focused on the promotion of neural progenitor cells and neurogenesis. However, it is now increasingly recognized that glial responses are critical for recovery in the entire neurovascular unit. In this study, we investigated the cellular effects of epidermal growth factor (EGF) containing hydrogels on primary astrocyte cultures. Both EGF alone and EGF‐hydrogel equally promoted astrocyte proliferation, but EGF‐hydrogels further enhanced astrocyte activation, as evidenced by a significantly elevated Glial fibrillary acidic protein (GFAP) gene expression. Thereafter, conditioned media from astrocytes activated by EGF‐hydrogel protected neurons against injury and promoted synaptic plasticity after oxygen–glucose deprivation. Taken together, these findings suggest that EGF‐hydrogels can shift astrocytes into neuro‐supportive phenotypes. Consistent with this idea, quantitative‐polymerase chain reaction (qPCR) demonstrated that EGF‐hydrogels shifted astrocytes in part by downregulating potentially negative A1‐like genes (Fbln5 and Rt1‐S3) and upregulating potentially beneficial A2‐like genes (Clcf1, Tgm1, and Ptgs2). Further studies are warranted to explore the idea of using biomaterials to modify astrocyte behavior and thus indirectly augment neuroprotection and neuroplasticity in the context of stem cell and growth factor therapies for the CNS. Stem Cells Translational Medicine 2019;8:1242&1248
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- 2019
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4. Platelet-Rich Plasma Lysate-Incorporating Gelatin Hydrogel as a Scaffold for Bone Reconstruction
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Meral Nadra, Wanting Niu, Motoichi Kurisawa, Dominique Rousson, and Myron Spector
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biomaterial ,gelatin ,bone regeneration ,hydrogel ,growth factors ,tissue engineering ,Technology ,Biology (General) ,QH301-705.5 - Abstract
In implant dentistry, large vertical and horizontal alveolar ridge deficiencies in mandibular and maxillary bone are challenges that clinicians continue to face. One of the limitations of porous blocks for reconstruction of bone in large defects in the oral cavity, and in the musculoskeletal system, is that fibrin clot does not adequately fill the interior pores and does not persist long enough to accommodate cell migration into the center of the block. The objective of our work was to develop a gelatin-based gel incorporating platelet-rich plasma (PRP) lysate, to mimic the role that a blood clot would normally play to attract and accommodate the migration of host osteoprogenitor and endothelial cells into the scaffold, thereby facilitating bone reconstruction. A conjugate of gelatin (Gtn) and hydroxyphenyl propionic acid (HPA), an amino-acid-like molecule, was commended for this application because of its ability to undergo enzyme-mediated covalent cross-linking to form a hydrogel in vivo, after being injected as a liquid. The initiation and propagation of cross-linking were under the control of horseradish peroxidase and hydrogen peroxide, respectively. The objectives of this in vitro study were directed toward evaluating: (1) the migration of rat mesenchymal stem cells (MSCs) into Gtn–HPA gel under the influence of rat PRP lysate or recombinant platelet-derived growth factor (PDGF)-BB incorporated into the gel; (2) the differentiation of MSCs, incorporated into the gel, into osteogenic cells under the influence of PRP lysate and PDGF-BB; and (3) the release kinetics of PDGF-BB from gels incorporating two formulations of PRP lysate and recombinant PDGF-BB. Results: The number of MSCs migrating into the hydrogel was significantly (3-fold) higher in the hydrogel group incorporating PRP lysate compared to the PDGF-BB and the blank gel control groups. For the differentiation/osteogenesis assay, the osteocalcin-positive cell area percentage was significantly higher in both the gel/PRP and gel/PDGF-BB groups, compared to the two control groups: cells in the blank gels grown in cell expansion medium and in osteogenic medium. Results of the ELISA release assay indicated that Gtn–HPA acted as an effective delivery vehicle for the sustained release of PDGF-BB from two different PRP lysate batches, with about 60% of the original PDGF-BB amount in the two groups remaining in the gel at 28 days. Conclusions: Gtn–HPA accommodates MSC migration. PRP-lysate-incorporating hydrogels chemoattract increased MSC migration into the Gtn–HPA compared to the blank gel. PRP-lysate- and the PDGF-BB-incorporating gels stimulate osteogenic differentiation of the MSCs. The release of the growth factors from Gtn–HPA containing PRP lysate can extend over the period of time (weeks) necessary for bone reconstruction. The findings demonstrate that Gtn–HPA can serve as both a scaffold for cell migration and a delivery vehicle that allows sustained and controlled release of the incorporated therapeutic agent over extended periods of time. These findings commend Gtn–HPA incorporating PRP lysate for infusion into porous calcium phosphate blocks for vertical and horizontal ridge reconstruction, and for other musculoskeletal applications.
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- 2022
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5. Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis
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Pengfei Lei, Zixun Dai, Yu Shrike Zhang, Hua Liu, Wanting Niu, Kun Li, Long Wang, Yihe Hu, and Jie Xie
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Ultrahigh molecular weight polyethylene ,Wear particles ,Fibroblast ,Macrophage ,Osteogenesis ,Osteolysis ,Orthopedic surgery ,RD701-811 ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background In the ultrahigh molecular weight polyethylene (UHMWPE) prosthetic environment, fibroblasts affected by wear particles have the capacity of osteogenesis to reduce osteolysis. We aimed to assess the effects of macrophages on the osteogenic capability of fibroblasts treated with UHMWPE wear particles. Methods The effect of different concentrations of UHMWPE (0, 0.01, 0.1, and 1 mg/ml, respectively) on macrophage proliferation were validated by MTT assay to determine the optimum one. The fibroblasts viability was further determined in the co-culture system of UHMWPE particles and macrophage supernatants. The experiment was designed as seven groups: (A) fibroblasts only; (B) fibroblasts + 1 mg/ml UHMWPE particles; and (C1–C5) fibroblasts + 1/16, 1/8, 1/4, 1/2, and 1/1 supernatants of macrophage cultures stimulated by 1 mg/ml UHMWPE particles vs. fibroblast complete media, respectively. Alizarin red staining was used to detect calcium accumulation. The expression levels of osteogenic proteins were detected by Western blot and ELISA, including alkaline phosphatase (ALP) and osteocalcin (OCN). Results The concentration of 0.1 mg/ml was considered as the optimum concentration for macrophage proliferation due to the survival rate and was highest among the four concentrations. Fibroblast viability was better in the group of fibroblasts + 1/16 ratio of macrophage supernatants stimulated by 1 mg/ml of UHMWPE particles than the other groups (1:8, 1:4, 1:2, 1:1). ALP and OCN expressions were significantly decreased in the group of fibroblasts + 1/4, 1/2, and 1/1 supernatants stimulated by 1 mg/ml of UHMWPE particles compared with other groups (1/8, 1/16) and the group of fibroblasts + 1 mg/ml UHMWPE (p
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- 2019
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6. Mangiferin Relieves Lipopolysaccharide-Induced Injury by Up-Regulating miR-181a via Targeting PTEN in ATDC5 Cells
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Yunfei Ma, Ying Liu, Yunyan Ma, Nan Jiang, Lei Wang, Bowei Wang, Wanting Niu, Yanjun Hu, Qingrong Lin, and Bin Yu
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osteoarthritis ,mangiferin ,PTEN ,miR-181a ,NF-κB ,PTEN/PI3K/AKT pathways ,Therapeutics. Pharmacology ,RM1-950 - Abstract
BackgroundMangiferin (MF) was reported to possess anti-inflammatory activity. This investigation tried to probe into the underlying mechanism of MF in osteoarthritis.MethodsATDC5 cells were pretreated with series concentrations of MF (0.1, 1, 5, 10, 15, 20 μM) for 2 h and then were exposed to lipopolysaccharide (LPS) (5 μg/ml) for 12 h to construct the inflammatory injury model. The cell viability, productions of pro-inflammatory cytokines and enzymes were respectively measured by employing CCK-8 assay, western blot, ELISA, and quantitative reverse-transcription (qRT)-PCR. miR-181a expression was altered by employing cell transfection. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) method was employed for detection of reactive oxygen species (ROS) generation. Dual luciferase activity assay was conducted for analyzing the relationship between miR-181a and PTEN. The underlying mechanism was determined by employing western blot.ResultsHigh doses of MF treatment (15 and 20 μM) noticeably induced inflammatory injury exhibiting as increased the productions of pro-inflammatory cytokines, enzymes and ROS, activated NF-κB pathway and deactivated PTEN/PI3K/AKT pathway in ATDC5 cells. Besides, MF treatment notably remitted LPS-induced inflammatory injury through deactivation of NF-κB pathway and activation of PTEN/PI3K/AKT pathway. PTEN was a target of miR-181a. Inhibition of miR-181a remarkably reversed MF-triggered impacts on ATDC5 cells.ConclusionMF attenuated LPS-induced inflammatory damage through miR-181a/PTEN axis and thereby inhibiting NF-κB pathway and activating PI3K/AKT pathway.
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- 2020
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7. Cellular Stress and Molecular Responses in Bladder Ischemia
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Jing-Hua Yang, Han-Pil Choi, Wanting Niu, and Kazem M. Azadzoi
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bladder ischemia ,cellular stress ,post-translational modifications ,non-coded amino acids ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The concept of bladder ischemia as a contributing factor to detrusor overactivity and lower urinary tract symptoms (LUTS) is evolving. Bladder ischemia as a consequence of pelvic arterial atherosclerosis was first documented in experimental models and later in elderly patients with LUTS. It was shown that early-stage moderate ischemia produces detrusor overactivity, while prolonged severe ischemia provokes changes consistent with detrusor underactivity. Recent studies imply a central role of cellular energy sensors, cellular stress sensors, and stress response molecules in bladder responses to ischemia. The cellular energy sensor adenosine monophosphate-activated protein kinase was shown to play a role in detrusor overactivity and neurodegeneration in bladder ischemia. The cellular stress sensors apoptosis signal-regulating kinase 1 and caspase-3 along with heat shock proteins were characterized as important contributing factors to smooth muscle structural modifications and apoptotic responses in bladder ischemia. Downstream pathways seem to involve hypoxia-inducible factor, transforming growth factor beta, vascular endothelial growth factor, and nerve growth factor. Molecular responses to bladder ischemia were associated with differential protein expression, the accumulation of non-coded amino acids, and post-translational modifications of contractile proteins and stress response molecules. Further insight into cellular stress responses in bladder ischemia may provide novel diagnostic and therapeutic targets against LUTS.
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- 2021
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8. Reconstruction of Large-scale Defects with a Novel Hybrid Scaffold Made from Poly(L-lactic acid)/Nanohydroxyapatite/Alendronate-loaded Chitosan Microsphere: in vitro and in vivo Studies
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Hongwei Wu, Pengfei Lei, Gengyan Liu, Yu Shrike Zhang, Jingzhou Yang, Longbo Zhang, Jie Xie, Wanting Niu, Hua Liu, Jianming Ruan, Yihe Hu, and Chaoyue Zhang
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Medicine ,Science - Abstract
Abstract A chitosan-based microsphere delivery system has been fabricated for controlled release of alendronate (AL). The present study aimed to incorporate the chitosan/hydroxyapatite microspheres-loaded with AL (CH/nHA-AL) into poly(L-lactic acid)/nanohydroxyapatite (PLLA/nHA) matrix to prepare a novel microspheres-scaffold hybrid system (CM-ALs) for drug delivery and bone tissue engineering application. The characteristics of CM-ALs scaffolds containing 10% and 20% CH/nHA-AL were evaluated in vitro, including surface morphology and porosity, mechanical properties, drug release, degradation, and osteogenic differentiation. The in vivo bone repair for large segmental radius defects (1.5 cm) in a rabbit model was evaluated by radiography and histology. In vitro study showed more sustained drug release of CM-AL-containing scaffolds than these of CM/nHA-AL and PLLA/nHA/AL scaffolds, and the mechanical and degradation properties of CM-ALs (10%) scaffolds were comparable to that of PLLA/nHA control. The osteogenic differentiation of adipose-derived stem cells (ASCs) was significantly enhanced as indicated by increased alkaline phosphates (ALP) activity and calcium deposition. In vivo study further showed better performance of CM-ALs (10%) scaffolds with complete repair of large-sized bone defects within 8 weeks. A microspheres-scaffold-based release system containing AL-encapsulated chitosan microspheres was successfully fabricated in this study. Our results suggested the promising application of CM-ALs (10%) scaffolds for drug delivery and bone tissue engineering.
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- 2017
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9. In Situ Cross-linking Hydrogel as a Vehicle for Retinal Progenitor Cell Transplantation
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Jeayoung Park, Petr Baranov, Aybike Aydin, Hany Abdelgawad, Deepti Singh, Wanting Niu, Motoichi Kurisawa, Myron Spector, and Michael J. Young
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Medicine - Abstract
One of the current limitations of retinal transplantation of stem cells as well as other cell types is the dispersion of cells from the injection site (including loss of cells into the vitreous chamber) and low survival after transplantation. Gelatin-hydroxyphenyl propionic acid (Gtn-HPA) conjugate is a biodegradable polymer that can undergo covalent cross-linking in situ , allowing for injection of incorporated cells through a small caliber needle followed by gel formation in vivo . We tested the hypothesis that Gtn-HPA hydrogel supports survival and integration of retinal progenitor cells (RPCs) post-transplantation. In vitro compatibility and in vivo graft survival were assessed by mixing an equal volume of Gtn-HPA conjugate and RPC suspension and triggering enzyme-mediated gelation, using minute amounts of horseradish peroxidase and peroxide. Immunocytochemistry showed >80% survival of cells and minimal apoptosis for cells incorporated into Gtn-HPA, equivalent to controls grown on fibronectin-coated flasks. RPCs undergoing mitosis were seen within the three-dimensional Gtn-HPA hydrogel, but the percentage of Ki-67-positive cells was lower compared with the monolayer controls. For in vivo studies, gel–cell mixture or cell suspension in saline was trans-sclerally injected into the left eye of female Long Evans rats immunosuppressed with cyclosporine A. Grafts survived at the 1 week time point of the study, with Gtn-HPA-delivered grafts showing less inflammatory response demonstrated by anti-leukocyte staining. More eyes in the gel–cell mixture group showed surviving cells in the subretinal space compared with saline-delivered controls, while the number of cells surviving per graft was not significantly different between the two groups. This work demonstrates an injectable in situ cross-linking hydrogel as a potential vehicle for stem cell delivery in the retina.
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- 2019
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10. Platelet-Derived Growth Factor Stimulated Migration of Bone Marrow Mesenchymal Stem Cells into an Injectable Gelatin-Hydroxyphenyl Propionic Acid Matrix
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Wanting Niu, Teck Chuan Lim, Abdulmonem Alshihri, Ravikumar Rajappa, Lishan Wang, Motoichi Kurisawa, and Myron Spector
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bone marrow mesenchymal stem cells ,migration ,proliferation ,platelet-derived growth factor ,stromal cell-derived factor-1α ,Biology (General) ,QH301-705.5 - Abstract
Bone marrow mesenchymal stem cells (bMSCs) are responsible in the repair of injured tissue through differentiation into multiple cell types and secretion of paracrine factors, and thus have a broad application profile in tissue engineering/regenerative medicine, especially for the musculoskeletal system. The lesion due to injury or disease may be a closed irregular-shaped cavity deep within tissue necessitating an injectable biomaterial permissive of host (endogenous) cell migration, proliferation and differentiation. Gelatin-hydroxyphenyl propionic acid (Gtn-HPA) is a natural biopolymer hydrogel which is covalently cross-linked by horseradish peroxidase (HRP) and hydrogen peroxide (H2O2) in situ and can be delivered to the lesion by needle injection. Growth factors and cytokines can be directly incorporated into the gel or into nano- and micro-particles, which can be employed for sustained release of biomolecules while maintaining their bioactivity. In this study, we selected polyelectrolyte complex nanoparticles (PCNs) prepared with dextran sulfate and chitosan as the carrier for platelet-derived growth factor (PDGF)-BB and stromal cell-derived factor (SDF)-1α, which have been tested effectively in recruiting stem cells. Our in vitro results showed a high degree of viability of bMSCs through the process of Gtn-HPA covalent cross-linking gelation. The Gtn-HPA matrix was highly permissive of bMSC migration, proliferation, and differentiation. PDGF-BB (20 ng/mL) directly incorporated into the gel and, alternatively, released from PCNs stimulated bMSC migration and proliferation. There were only small differences in the results for the direct incorporation of PDGF into the gel compared with its release from PCNs, and for increased doses of the growth factor (200 ng/mL and 2 µg/mL). In contrast, SDF-1α elicited an increase in migration and proliferation only when released from PCNs; its effect on migration was notably less than PDGF-BB. The in vitro results demonstrate that PDGF-BB substantially increases migration of bMSCs into Gtn-HPA and their proliferation in the gel, and that these benefits can be derived from incorporation of a relatively low dose of the growth factor directly into the gel. These findings commend the use of Gtn-HPA/PDGF-BB as an injectable therapeutic agent to treat defects in musculoskeletal tissues.
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- 2021
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11. Bisphenol S exposure promotes cell apoptosis and mitophagy in murine osteocytes by regulating <scp>mtROS</scp> signaling
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Weiyan Shan, Wanting Niu, Qiao Lin, Yuchen Shen, Fangmin Shen, Kai Lou, and Yun Zhang
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Medical Laboratory Technology ,Histology ,Anatomy ,Instrumentation - Published
- 2023
12. Formation of Double Stranded RNA Provokes Smooth Muscle Contractions and Structural Modifications in Bladder Ischemia
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Jing-Hua Yang, Zuohui Zhao, Wanting Niu, Han-Pil Choi, and Kazem M Azadzoi
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Research and Reports in Urology ,Urology - Abstract
Jing-Hua Yang,1 Zuohui Zhao,2 Wanting Niu,3 Han-Pil Choi,3 Kazem M Azadzoi4 1Department of Surgery, Boston University School of Medicine and Proteomics Laboratory, VA Boston Healthcare System, Boston, MA, USA; 2Department of Urology, Boston University School of Medicine, Boston, MA, USA; 3Research Department, VA Boston Healthcare System, Boston, MA, USA; 4Departments of Urology and Pathology, VA Boston Healthcare System and Boston University School of Medicine, Boston, MA, USACorrespondence: Kazem M Azadzoi, VA Boston Healthcare System, Building 1A, Room 317 (151), 150 South Huntington Avenue, Boston, MA, 02130, USA, Tel +1 857-364-5602, Email kazadzoi@bu.eduPurpose: Growing evidence suggests that ischemia provokes detrusor overactivity and degenerative responses in the bladder. Underlying mechanisms appear to involve modification of smooth muscle contractile rudiments by hypoxia, redox, cellular stress and cell survival signaling. Downstream pathways of cellular stress and stress response molecules eliciting bladder dysfunction in ischemia remain largely elusive. Our goal was to define the role of double stranded RNA (dsRNA), a stress response molecule provoked by redox, in ischemia mediated bladder dysfunction.Methods: A rat model of pelvic ischemia along with a cell culture hypoxia model were used to investigate the expression levels, functional consequences, structural aspects, and regulatory mechanisms of dsRNA in the bladder. Gene and protein expression were examined by reverse transcription polymerase chain reaction (RT-PCR), dot blot, and Western blotting, respectively. Tissue structure and function were assessed using histological staining and organ bath. Regulatory mechanisms were analyzed in cultured bladder smooth muscle cells.Results: The data presented here provide the first evidence of the formation of dsRNA in the overactive bladder. dsRNA is a cellular stress response molecule that sensitizes smooth muscle and regulates inflammatory and degenerative rejoinders. Our data suggest that the production of dsRNA in the bladder is provoked by ischemia. Formation of dsRNA appears to augment bladder smooth muscle contractions and provoke fibrotic and apoptotic responses. Downstream actions of dsRNA in the bladder may involve upregulation of dsRNA-activated protein kinase R (PKR) and caspase-3, the executioner of apoptosis.Conclusion: Activation of dsRNA/PKR pathway may play a role in sensitization of bladder smooth muscle cells to contractile stimuli, whereas dsRNA and caspase-3 crosstalk appear to modulate cellular stress and instigate degenerative responses in bladder ischemia. These observations suggest the role of dsRNA in bladder dysfunction and may open new perspectives to overcome overactive smooth muscle contractions and structural damage in the bladder.Keywords: bladder, ischemia, overactivity, dsRNA, contraction, degeneration
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- 2022
13. The effects of shock wave stimulation of mesenchymal stem cells on proliferation, migration, and differentiation in an injectable gelatin matrix for osteogenic regeneration
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Atsushi Yamashita, Peer W. Kämmerer, Motoichi Kurisawa, Wanting Niu, Myron Spector, Mansour Al-Askar, and Abdulmonem Alshihri
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Cell Survival ,medicine.medical_treatment ,0206 medical engineering ,Biomedical Engineering ,Medicine (miscellaneous) ,Motility ,02 engineering and technology ,Matrix (biology) ,Injections ,Biomaterials ,03 medical and health sciences ,Cell Movement ,Osteogenesis ,In vivo ,medicine ,Animals ,Regeneration ,Cell Proliferation ,030304 developmental biology ,Electroshock ,0303 health sciences ,biology ,Chemistry ,Goats ,Growth factor ,Regeneration (biology) ,Mesenchymal stem cell ,Endoglin ,Cell Differentiation ,Mesenchymal Stem Cells ,020601 biomedical engineering ,Cell biology ,Culture Media, Conditioned ,biology.protein ,Gelatin ,Propionates ,Stem cell ,Biomarkers ,Platelet-derived growth factor receptor - Abstract
The treatment of a variety of defects in bony sites could benefit from mitogenic stimulation of osteoprogenitor cells, including endogenous bone marrow-derived mesenchymal stem cells (bMSCs), and from provision of such cells with a matrix permissive of their migration, proliferation, and osteogenic differentiation. That such MSC stimulation could result from treatment with noninvasive (extracorporeal) shock waves (ESWs), and the matrix delivered by injection could enable this therapeutic approach to be employed for applications in which preformed scaffolds and growth factor therapy are difficult to deploy. The objectives of the present study were to investigate focused ESWs for their effects on proliferation, migration, and osteogenic differentiation in an injectable gelatin (Gtn) matrix capable of undergoing covalent cross-linking in vivo. Gtn was conjugated with hydroxyphenyl propionic acid (HPA) in order to enable it to be covalently cross-linked with minute amounts of horseradish peroxidase and hydrogen peroxide. The results demonstrated that 500 shocks of 0.4-mJ/mm2 energy flux density resulted in a twofold greater proliferation of bMSCs in the Gtn-HPA matrix after 14 days, compared with bMSCs grown with supplementation with platelet-derived growth factor (PDGF)-BB, a known mitogen for bMSCs. Moreover, SW treatment enhanced substantially osteogenic differentiation of bMSCs. The Gtn-HPA gel was permissive of MSC migration under the chemotactic influence of the growth factor, PDGF-BB, incorporated into and released by the gel. ESW treatment had no effect on the motility of the MSCs. The findings of the study warrant further investigation of this combined treatment modality for select bony defects.
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- 2020
14. Vascular Endothelial Growth Factor 165-Binding Heparan Sulfate Promotes Functional Recovery From Cerebral Ischemia
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Shuzhen Guo, Victor Nurcombe, Emiri Mandaville, Su Jing Chan, Wanting Niu, Eng H. Lo, Elga Esposito, Peter Tsun-Hong Wong, Raymond A. A. Smith, Simon M. Cool, and Kazuhide Hayakawa
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Male ,Vascular Endothelial Growth Factor A ,Angiogenesis ,Ischemia ,Neovascularization, Physiologic ,Vascular permeability ,Blood–brain barrier ,Brain Ischemia ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Neural Stem Cells ,medicine ,Animals ,Cell Proliferation ,Injections, Intraventricular ,030304 developmental biology ,Advanced and Specialized Nursing ,0303 health sciences ,business.industry ,Neurogenesis ,Infarction, Middle Cerebral Artery ,Recovery of Function ,Heparan sulfate ,Nestin ,medicine.disease ,Vascular Endothelial Growth Factor Receptor-2 ,Rats ,Cell biology ,Stroke ,Endothelial stem cell ,medicine.anatomical_structure ,chemistry ,Blood-Brain Barrier ,Ischemic Attack, Transient ,Heparitin Sulfate ,Neurology (clinical) ,Cardiology and Cardiovascular Medicine ,business ,030217 neurology & neurosurgery - Abstract
Background and Purpose: Although VEGF 165 (vascular endothelial growth factor-165) is able to enhance both angiogenesis and neurogenesis, it also increases vascular permeability through the blood-brain barrier. Heparan sulfate (HS) sugars play important roles in regulating VEGF bioactivity in the pericellular compartment. Here we asked whether an affinity-purified VEGF 165 -binding HS (HS7) could augment endogenous VEGF activity during stroke recovery without affecting blood-brain barrier function. Methods: Both rat brain endothelial cell line 4 and primary rat neural progenitor cells were used to evaluate the potential angiogenic and neurogenic effects of HS7 in vitro. For in vivo experiments, male Sprague-Dawley rats were subjected to 100 minutes of transient focal cerebral ischemia, then treated after 4 days with either PBS or HS7. One week later, infarct volume, behavioral sequelae, immunohistochemical markers of angiogenesis and neural stem cell proliferation were assessed. Results: HS7 significantly enhanced VEGF 165 -mediated angiogenesis in rat brain endothelial cell line 4 brain endothelial cells, and increased the proliferation and differentiation of primary neural progenitor cells, both via the VEGFR2 (vascular endothelial growth factor receptor 2) pathway. Intracerebroventricular injection of HS7 improved neurological outcome in ischemic rats without changing infarct volumes. Immunostaining of the compromised cerebrum demonstrated increases in collagen IV/Ki67 and nestin/Ki67 after HS7 exposure, consistent with its ability to promote angiogenesis and neurogenesis, without compromising blood-brain barrier integrity. Conclusions: A VEGF-activating glycosaminoglycan sugar, by itself, is able to enhance endogenous VEGF 165 activity during the post-ischemic recovery phase of stroke.
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- 2020
15. Endothelial cells regulate astrocyte to neural progenitor cell trans-differentiation in a mouse model of stroke
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Wenlu Li, Emiri T. Mandeville, Violeta Durán-Laforet, Norito Fukuda, Zhanyang Yu, Yi Zheng, Aaron Held, Ji-Hyun Park, Takafumi Nakano, Masayoshi Tanaka, Jingfei Shi, Elga Esposito, Wanting Niu, Changhong Xing, Kazuhide Hayakawa, Ignacio Lizasoain, Klaus van Leyen, Xunming Ji, Brian J. Wainger, Maria A. Moro, and Eng H. Lo
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Male ,Stroke ,Mice ,Multidisciplinary ,Neural Stem Cells ,Astrocytes ,Cell Transdifferentiation ,General Physics and Astronomy ,Animals ,Endothelial Cells ,General Chemistry ,General Biochemistry, Genetics and Molecular Biology ,Cells, Cultured - Abstract
The concept of the neurovascular unit emphasizes the importance of cell-cell signaling between neural, glial, and vascular compartments. In neurogenesis, for example, brain endothelial cells play a key role by supplying trophic support to neural progenitors. Here, we describe a surprising phenomenon where brain endothelial cells may release trans-differentiation signals that convert astrocytes into neural progenitor cells in male mice after stroke. After oxygen-glucose deprivation, brain endothelial cells release microvesicles containing pro-neural factor Ascl1 that enter into astrocytes to induce their trans-differentiation into neural progenitors. In mouse models of focal cerebral ischemia, Ascl1 is upregulated in endothelium prior to astrocytic conversion into neural progenitor cells. Injecting brain endothelial-derived microvesicles amplifies the process of astrocyte trans-differentiation. Endothelial-specific overexpression of Ascl1 increases the local conversion of astrocytes into neural progenitors and improves behavioral recovery. Our findings describe an unexpected vascular-regulated mechanism of neuroplasticity that may open up therapeutic opportunities for improving outcomes after stroke.
- Published
- 2021
16. Brain Delivery of Curcumin Through Low-Intensity Ultrasound-Induced Blood-Brain Barrier Opening via Lipid-PLGA Nanobubbles
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Wanting Niu, Haifeng Liang, Liming Song, Feiyan Cai, Zhiwen Su, Zhongxun Liu, Fei Yan, Yiran Yan, Yan Chen, and Bo Yu
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Parkinson's disease ,Curcumin ,Central nervous system ,Biophysics ,Pharmaceutical Science ,Bioengineering ,Pharmacology ,Blood–brain barrier ,blood–brain barrier ,Biomaterials ,chemistry.chemical_compound ,Mice ,International Journal of Nanomedicine ,Low intensity ultrasound ,In vivo ,Drug Discovery ,medicine ,Animals ,Particle Size ,Ultrasonography ,Original Research ,Chemistry ,low-intensity focus ultrasound ,Organic Chemistry ,food and beverages ,Brain ,General Medicine ,medicine.disease ,Lipids ,Bioavailability ,Mice, Inbred C57BL ,PLGA ,medicine.anatomical_structure ,Blood-Brain Barrier ,Parkinson’s disease ,Nanoparticles ,nanobubbles - Abstract
Yiran Yan,1,* Yan Chen,2,* Zhongxun Liu,1 Feiyan Cai,3 Wanting Niu,4,5 Liming Song,1 Haifeng Liang,1 Zhiwen Su,1 Bo Yu,1 Fei Yan6 1Department of Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510282, Peopleâs Republic of China; 2Department of Ultrasonic Diagnosis, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510282, Peopleâs Republic of China; 3Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, Peopleâs Republic of China; 4VA Boston Healthcare System, Boston, MA, 02130, USA; 5Department of Orthopedics, Brigham and Womenâs Hospital, Harvard Medical School, Boston, MA, 02115, USA; 6CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Fei YanShenzhen Institutes of Advance Technology, Chinese Academy of Science, 1068 Xueyuan Avenue, Shenzhen University Town, Shenzhen, Guangdong, 518055, Peopleâs Republic of ChinaTel +86 755 86392284Fax +86 755 96382299Email fei.yan@siat.ac.cnBo YuDepartment of Orthopedics, Zhujiang Hospital, Southern Medical University, 253 Gongye Avenue, Haizhu District, Guangzhou, Guangdong, 510280, Peopleâs Republic of ChinaTel +86 20 67282573Fax +86 20 61643010Email gzyubo@163.comBackground: Parkinsonâs disease (PD) is a progressive neurodegenerative disorder. Owing to the presence of bloodâbrain barrier (BBB), conventional pharmaceutical agents are difficult to the diseased nuclei and exert their action to inhibit or delay the progress of PD. Recent literatures have demonstrated that curcumin shows the great potential to treat PD. However, its applications are still difficult in vivo due to its poor druggability and low bioavailability through the BBB.Methods: Melt-crystallization methods were used to improve the solubility of curcumin, and curcumin-loaded lipid-PLGA nanobubbles (Cur-NBs) were fabricated through encapsulating the curcumin into the cavity of lipid-PLGA nanobubbles. The bubble size, zeta potentials, ultrasound imaging capability and drug encapsulation efficiency of the Cur-NBs were characterized by a series of analytical methods. Low-intensity focused ultrasound (LIFU) combined with Cur-NB was used to open the BBB to facilitate curcumin delivery into the deep brain of PD mice, followed by behavioral evaluation for the treatment efficacy.Results: The solubility of curcumin was improved by melt-crystallization methods, with 2627-fold higher than pure curcumin. The resulting Cur-NBs have a nanoscale size about 400 nm and show excellent contrast imaging performance. Curcumin drugs encapsulated into Cur-NBs could be effectively released when Cur-NBs were irradiated by LIFU at the optimized acoustic pressure, achieving 30% cumulative release rate within 6 h. Importantly, Cur-NBs combined with LIFU can open the BBB and locally deliver the curcumin into the deep-seated brain nuclei, significantly enhancing efficacy of curcumin in the Parkinson C57BL/6J mice model in comparison with only Cur-NBs and LIFU groups.Conclusion: In this work, we greatly improved the solubility of curcumin and developed Cur-NBs for brain delivery of curcumin against PD through combining with LIFU-mediating BBB. Cur-NBs provide a platform for these potential drugs which are difficult to cross the BBB to treat PD disease or other central nervous system (CNS) diseases.Keywords: nanobubbles, bloodâbrain barrier, low-intensity focus ultrasound, curcumin, Parkinsonâs disease
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- 2021
17. Tricalcium phosphate particles promote pyroptotic death of calvaria osteocytes through the ROS/NLRP3/Caspase-1 signaling axis in amouse osteolysis model
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Yun, Zhang, Ming, Yan, Wanting, Niu, Hongjiao, Mao, Pei, Yang, Bingbing, Xu, and Yonghong, Sun
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Calcium Phosphates ,Pharmacology ,Inflammasomes ,Caspase 1 ,Skull ,Immunology ,NLR Proteins ,Osteolysis ,Osteocytes ,Mice ,NLR Family, Pyrin Domain-Containing 3 Protein ,Pyroptosis ,Animals ,Immunology and Allergy ,Reactive Oxygen Species - Abstract
Wear particles-induced inflammatory osteolysis, a major factor of aseptic loosening affects the long-term survival of orthopedic prostheses. Increasing observations have demonstrated that osteocytes, making up over 95% of all the bone cells, is involved in wear particle-induced periprosthetic osteolysis, but its mechanism remains unclear. In the present study, we embedded micro-sized tricalcium phosphate (TCP) particles (30 mg) under the periosteum around the middle suture of the mouse calvaria to establish a calvarial osteolysis model and investigated the biological effects of the particles on calvaria osteocytes in vivo. Results showed that TCP particles induced pyroptosis and activated the NLRP3 inflammasome in calvaria osteocytes, which was confirmed by obvious increases in empty lacunae, protein expressions of speck-like protein containing CARD (ASC), NOD-like receptor protein 3 (NLRP3), cleaved caspase-1 (Casp-1 p20) and cleaved gasdermin D (GSDMD-N), and resulted in elevated ratios of Casp-1 p20/Casp-1 and interleukin (IL)-1β/pro-IL-1β. Simultaneously, TCP particles enhanced serum levels of lactate dehydrogenase (LDH) and IL-1β. Furthermore, the pyroptotic effect was reversed by the Casp-1 inhibitor VX765 or the NLRP3 inhibitor MCC950. In addition, TCP particles increased the levels of intracellular reactive oxygen species (ROS) and malonaldehyde (MDA), whereas decreased the antioxidant enzyme nuclear factor E2-related factor 2 (Nrf2) level, leading to oxidative stress in calvaria osteocytes; the ROS scavenger N-acetylcysteine (NAC) attenuated these effects of pyroptotic death and the NLPR3 activation triggered by TCP particles. Collectively, our data suggested that TCP particles promote pyroptotic death of calvaria osteocytes through the ROS/NLRP3/Caspase-1 signaling axis, contributing to osteoclastogenesis and periprosthetic osteolysis.
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- 2022
18. Paeonol attenuates isoflurane anesthesia-induced hippocampal neurotoxicity via modulation of JNK/ERK/P38MAPK pathway and regulates histone acetylation in neonatal rat
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Haiyan Jin, Wanting Niu, Jiangmei Wang, Hongmin Cao, Minyan Wang, and Lizhong Du
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Male ,0301 basic medicine ,MAPK/ERK pathway ,MAP Kinase Signaling System ,Hippocampal formation ,Pharmacology ,Hippocampus ,p38 Mitogen-Activated Protein Kinases ,Histones ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Pregnancy ,Animals ,Medicine ,Anesthesia ,Anesthetics ,Histone Acetyltransferases ,Neurons ,Isoflurane ,business.industry ,Neurodegeneration ,JNK Mitogen-Activated Protein Kinases ,Neurotoxicity ,Acetophenones ,Obstetrics and Gynecology ,Acetylation ,medicine.disease ,Rats ,Neuroprotective Agents ,030104 developmental biology ,Animals, Newborn ,chemistry ,Pediatrics, Perinatology and Child Health ,Female ,Neurotoxicity Syndromes ,Histone deacetylase ,Paeonol ,business ,030217 neurology & neurosurgery ,Signal Transduction ,medicine.drug - Abstract
Objective: Volatile anesthetic such as isoflurane causes widespread neurodegeneration in the developing animal brains and also induces cognitive impairments. Paeonol is a plant-derived phenolic com...
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- 2018
19. ROS/NLRP3/Caspase-1 Pathway Contributes to TCP Particles-Induced Pyroptosis of Calvaria Osteocytes in the Mouse Osteolysis Model
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Yun Zhang, Ming Yan, Wanting Niu, Jian Fang, Hongjiao Mao, and yonghong sun
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- 2021
20. Laparoscopic and Robotic-Assisted Partial Nephrectomy: An Overview of Hot Issues
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Guoan Zhao, Yongsheng Song, Zeqi Liu, Bin Wu, Wanting Niu, Lulin Ma, Ming Li, Renge Bu, Peihe Liang, Liang Cheng, Hongxian Zhang, Yan Song, and Ying Wang
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medicine.medical_specialty ,Robotic assisted ,Urology ,medicine.medical_treatment ,030232 urology & nephrology ,Complete resection ,Nephrectomy ,03 medical and health sciences ,0302 clinical medicine ,Robotic Surgical Procedures ,Renal cell carcinoma ,Medicine ,Humans ,Laparoscopy ,Intraoperative imaging ,Carcinoma, Renal Cell ,medicine.diagnostic_test ,business.industry ,Renal tumor ,medicine.disease ,Kidney Neoplasms ,Surgery ,030220 oncology & carcinogenesis ,business - Abstract
Laparoscopic partial nephrectomy and robot-assisted partial nephrectomy are attracting increased attention from urologists. They can achieve the same effect of oncology control as radical nephrectomy; moreover, they can offer better preservation of renal function, thus obtaining long-term living benefits. The indications are also expanding, making it possible for larger and more difficult tumors. Laparoscopic partial nephrectomy and robot-assisted partial nephrectomy can be performed by transperitoneal and retroperitoneal approaches, with their individual advantages and limitations. In addition, the renal tumor scoring systems have been widely used and studied in laparoscopic partial nephrectomy and robot-assisted partial nephrectomy. In order to better preserve renal function, the zero-ischemia technique is widely used. The application of intraoperative imaging technology provides convenience and greater benefits. Besides, whether minimal invasive partial nephrectomy can be performed without stop antiplatelet treatment is still disputed. Clinicians perform substantial exploration and practice to achieve the “trifecta” of surgery: complete resection of the tumor, maximum protection of renal function, and no complications.
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- 2020
21. Contributors
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Mohammad Hossein Alaei, Nafiseh Baheiraei, Hamideh Barghamadi, Behzad Bolandi, Shahin Bonakdar, Atefeh Derakhshani, Ronghui Du, Maryam Ghaffari, Mahnaz Gholami, Saeedeh Hasani, Hao Hong, Shuxian Huo, Rana Imani, Farzaneh Khamseh, Ge Li, Saeid Mollazadeh-Bajestani, Ali Mousavi, Fatholah Moztarzadeh, Mohammed Najafi-Ashtiani, Tina Navaei, Wanting Niu, Shirin Nour, Hanif Goran Orimi, Zahra Pasandideh, Soheila Pourkhodadad, Sara Tabatabaee, and Maryam Tajabadi
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- 2020
22. Nanotoxicity and regulatory aspects in musculoskeletal regeneration
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Ronghui Du, Wanting Niu, Shuxian Huo, and Hao Hong
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Tissue engineering ,Biocompatibility ,Nanotoxicology ,Chemistry ,law ,bacteria ,Protein Corona ,Nanotechnology ,Musculoskeletal regeneration ,Nanoengineering ,Carbon nanotube ,Nanomaterials ,law.invention - Abstract
We reviewed nanotoxicity and regulatory aspects of nanoengineering and nanomaterials in musculoskeletal regeneration. The chapter includes host response, biocompatibility, safety, and processing environment. Acute immune response, chronic immune response, and activation of immune cells were introduced in host response. Physiochemical parameters of nanomaterials impacting the biocompatibility including particle size and surface charge, shape and conjugation, protein corona, and activation of coagulation were summarized. We also introduced cell-biomaterial interaction and methods to determine the biocompatibility. To assess the safety of nanoparticles (NP), physicochemical parameters, biodegradation, incubation condition, toxicity species, assay methods of toxicity, and toxicity mechanisms are considered. Furthermore, we illustrated the safety of carbon nanotubes, fullerenes, graphene, and metallic nanoparticles that are used widely. In addition, tissue engineering, micro- and nanofabrication technology, stem-cell engineering, and 3-D environment were illustrated in processing environment.
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- 2020
23. Challenges toward musculoskeletal injuries and diseases
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Ge Li and Wanting Niu
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medicine.medical_specialty ,Physical medicine and rehabilitation ,Movement system ,business.industry ,Existing Treatment ,Orthopedic surgery ,medicine ,Arthritis ,medicine.disease ,business ,Clinical treatment ,Degenerative disc disease - Abstract
Musculoskeletal system belongs to the movement system, which is composed of bone, joint, and skeletal muscle. It is the main part of the body and plays the role of supporting the body, protecting the important organs, and moving. In this chapter, we will understand the composition of musculoskeletal system, the characteristics of musculoskeletal system injuries and diseases (including work-related musculoskeletal diseases, arthritis, and degenerative disc disease), and the failure cases of orthopedic implants. Learning the occurrence and pathophysiology of musculoskeletal system and the existing treatment schemes, problems, and challenges will provide a more comprehensive perspective for the renewal of clinical treatment.
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- 2020
24. Abnormal changes in voltage-gated sodium channels subtypes Na V 1.1, Na V 1.2, Na V 1.3, Na V 1.6 and CaM/CaMKII pathway in low-grade astrocytoma
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Zhi Li, Wanting Niu, Feng Guo, Liming Lei, Mingyi Zhao, Qinghua Gao, Jianing Li, Xiaoxue Xu, Dongyi Zhao, Tomasz Boczek, Gefei Guan, and Xiao-Yuan Mao
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0301 basic medicine ,Calmodulin ,biology ,business.industry ,General Neuroscience ,Sodium channel ,Astrocytoma ,Human brain ,medicine.disease ,03 medical and health sciences ,Epilepsy ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,nervous system ,Ca2+/calmodulin-dependent protein kinase ,NAV1 ,medicine ,Cancer research ,biology.protein ,Immunohistochemistry ,business ,030217 neurology & neurosurgery - Abstract
Epileptic seizures are the main clinical manifestation of low-grade astrocytoma. Voltage-gated sodium channels (VGSCs) play a crucial role in epilepsy. Until now, the role of VGSCs and the relationships between calmodulin (CaM)/CaM-dependent protein kinase II (CaMKII) and VGSCs in low-grade astrocytoma have not been demonstrated. In our study, the protein expression of NaV1.3, NaV1.6 and CaM was significantly increased in the tumor compared to control tissue, while the level of p-CaMKII/CaMKII was significantly decreased in the tumor group as determined by Western Blotting and immunohistochemistry. Furthermore, double-labeling immunofluorescence results showed that NaV1.3/NaV1.6 and CaM co-localization was significantly increased in the tumor group compared to control tissue. This study represents the first evidence of the abnormal changes in VGSCs subtypes and CaM/CaMKII pathway in human brain low-grade astrocytoma, providing new potential targets for molecular therapies of this disease.
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- 2018
25. Effect of Stimulation by Ultrahigh Molecular Weight Polyethylene Wear Particles on the Osteogenesis Capability of Fibroblasts
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Kun Li, Pengfei Lei, Hua Liu, Long Wang, Jie Xie, Zixun Dai, Wanting Niu, Yu Shrike Zhang, and Hu Yihe
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030203 arthritis & rheumatology ,030222 orthopedics ,03 medical and health sciences ,Ultrahigh molecular weight polyethylene ,0302 clinical medicine ,Materials science ,Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,Stimulation ,Biotechnology ,Biomedical engineering - Published
- 2018
26. Long non-coding RNAs in renal cell carcinoma: A systematic review and clinical implications
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Liang Cheng, Renge Bu, Bin Wu, Jun Huo, Jithin K. Raju, Ming Li, Ying Wang, Wanting Niu, Yongsheng Song, Bo Yin, and Guoan Zhao
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0301 basic medicine ,renal cell carcinoma ,medicine.medical_specialty ,Veterinary medicine ,diagnosis ,Medical laboratory ,Review ,urologic and male genital diseases ,03 medical and health sciences ,lncRNA ,0302 clinical medicine ,Beijing ,Renal cell carcinoma ,Biomarkers, Tumor ,medicine ,Humans ,Neoplasm Metastasis ,General hospital ,China ,Carcinoma, Renal Cell ,Neoplasm Staging ,therapy ,business.industry ,Advanced stage ,Medical school ,Prognosis ,medicine.disease ,Molecular biomarkers ,Kidney Neoplasms ,Gene Expression Regulation, Neoplastic ,Cell Transformation, Neoplastic ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Family medicine ,RNA, Long Noncoding ,Neoplasm Grading ,business - Abstract
// Ming Li 1 , Ying Wang 2, 3 , Liang Cheng 4 , Wanting Niu 5 , Guoan Zhao 6 , Jithin K. Raju 7 , Jun Huo 1 , Bin Wu 1 , Bo Yin 1 , Yongsheng Song 1 and Renge Bu 1 1 Department of Urology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China 2 Department of Nuclear Medicine, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China 3 Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA 4 Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA 5 Department of Orthopedics, Brigham and Women’s Hospital, VA Boston Healthcare System, Harvard Medical School, Boston, Massachusetts 02115, USA 6 School of Network Education, Beijing University of Posts and Telecommunications, Hebei, Beijing 100088, P.R. China 7 Department of Clinical Medicine, China Medical University, Shenyang, Liaoning 110122, P.R. China Correspondence to: Renge Bu, email: burg@sj-hospital.org Keywords: renal cell carcinoma, lncRNA, diagnosis, prognosis, therapy Received: November 18, 2016 Accepted: March 20, 2017 Published: April 12, 2017 ABSTRACT Renal cell carcinoma is one of the most common malignancy in adults, its prognosis is poor in an advanced stage and early detection is difficult due to the lack of molecular biomarkers. The identification of novel biomarkers for RCC is an urgent and meaningful project. Long non-coding RNA (lncRNA) is transcribed from genomic regions with a minimum length of 200 bases and limited protein-coding potential. Recently, lncRNAs have been greatly studied in a variety of cancer types. They participate in a wide variety of biological processes including cancer biology. In this review, we provide a new insight of the profiling of lncRNAs in RCC and their roles in renal carcinogenesis, with an emphasize on their potential in diagnosis, prognosis and potential roles in RCC therapy.
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- 2017
27. Mangiferin Relieves Lipopolysaccharide-Induced Injury by Up-Regulating miR-181a
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Qing-rong Lin, Nan Jiang, Bin Yu, Ying Liu, Lei Wang, Yanjun Hu, Bo-wei Wang, Wanting Niu, Yun-fei Ma, and Yunyan Ma
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0301 basic medicine ,PTEN ,Lipopolysaccharide ,miR-181a ,mangiferin ,NF-κB ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Western blot ,medicine ,Pharmacology (medical) ,Viability assay ,Mangiferin ,PI3K/AKT/mTOR pathway ,Original Research ,Pharmacology ,medicine.diagnostic_test ,biology ,lcsh:RM1-950 ,Transfection ,Molecular biology ,osteoarthritis ,lcsh:Therapeutics. Pharmacology ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,PTEN/PI3K/AKT pathways ,biology.protein - Abstract
Background Mangiferin (MF) was reported to possess anti-inflammatory activity. This investigation tried to probe into the underlying mechanism of MF in osteoarthritis. Methods ATDC5 cells were pretreated with series concentrations of MF (0.1, 1, 5, 10, 15, 20 μM) for 2 h and then were exposed to lipopolysaccharide (LPS) (5 μg/ml) for 12 h to construct the inflammatory injury model. The cell viability, productions of pro-inflammatory cytokines and enzymes were respectively measured by employing CCK-8 assay, western blot, ELISA, and quantitative reverse-transcription (qRT)-PCR. miR-181a expression was altered by employing cell transfection. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) method was employed for detection of reactive oxygen species (ROS) generation. Dual luciferase activity assay was conducted for analyzing the relationship between miR-181a and PTEN. The underlying mechanism was determined by employing western blot. Results High doses of MF treatment (15 and 20 μM) noticeably induced inflammatory injury exhibiting as increased the productions of pro-inflammatory cytokines, enzymes and ROS, activated NF-κB pathway and deactivated PTEN/PI3K/AKT pathway in ATDC5 cells. Besides, MF treatment notably remitted LPS-induced inflammatory injury through deactivation of NF-κB pathway and activation of PTEN/PI3K/AKT pathway. PTEN was a target of miR-181a. Inhibition of miR-181a remarkably reversed MF-triggered impacts on ATDC5 cells. Conclusion MF attenuated LPS-induced inflammatory damage through miR-181a/PTEN axis and thereby inhibiting NF-κB pathway and activating PI3K/AKT pathway.
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- 2019
28. Promoting Neuro-Supportive Properties of Astrocytes with Epidermal Growth Factor Hydrogels
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Wanting Niu, Myron Spector, Magdy Selim, Su Jing Chan, Eng H. Lo, Pike See Cheah, Zhangyang Yu, Shuzhen Guo, Ken Arai, Motoichi Kurisawa, Xiaoying Wang, Gen Hamanaka, and Kazuhide Hayakawa
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0301 basic medicine ,medicine.medical_treatment ,Biomaterials ,03 medical and health sciences ,0302 clinical medicine ,Neural Stem Cells ,Epidermal growth factor ,medicine ,Animals ,lcsh:QH573-671 ,Stroke recovery ,Cells, Cultured ,lcsh:R5-920 ,Glial fibrillary acidic protein ,biology ,Epidermal Growth Factor ,lcsh:Cytology ,Growth factor ,Neurogenesis ,Cell Differentiation ,Hydrogels ,Cell Biology ,General Medicine ,Neural stem cell ,Cell biology ,Rats ,030104 developmental biology ,medicine.anatomical_structure ,Neuroprotective Agents ,Astrocytes ,Synaptic plasticity ,biology.protein ,Reactive astrocytes ,Neuroplasticity ,Stem cell ,lcsh:Medicine (General) ,030217 neurology & neurosurgery ,Developmental Biology ,Astrocyte ,Tissue‐Specific Progenitor and Stem Cells - Abstract
Biomaterials provide novel platforms to deliver stem cell and growth factor therapies for central nervous system (CNS) repair. The majority of these approaches have focused on the promotion of neural progenitor cells and neurogenesis. However, it is now increasingly recognized that glial responses are critical for recovery in the entire neurovascular unit. In this study, we investigated the cellular effects of epidermal growth factor (EGF) containing hydrogels on primary astrocyte cultures. Both EGF alone and EGF‐hydrogel equally promoted astrocyte proliferation, but EGF‐hydrogels further enhanced astrocyte activation, as evidenced by a significantly elevated Glial fibrillary acidic protein (GFAP) gene expression. Thereafter, conditioned media from astrocytes activated by EGF‐hydrogel protected neurons against injury and promoted synaptic plasticity after oxygen–glucose deprivation. Taken together, these findings suggest that EGF‐hydrogels can shift astrocytes into neuro‐supportive phenotypes. Consistent with this idea, quantitative‐polymerase chain reaction (qPCR) demonstrated that EGF‐hydrogels shifted astrocytes in part by downregulating potentially negative A1‐like genes (Fbln5 and Rt1‐S3) and upregulating potentially beneficial A2‐like genes (Clcf1, Tgm1, and Ptgs2). Further studies are warranted to explore the idea of using biomaterials to modify astrocyte behavior and thus indirectly augment neuroprotection and neuroplasticity in the context of stem cell and growth factor therapies for the CNS. stem cells translational medicine 2019;8:1242&1248, Biomaterials provide novel platforms to deliver stem cell and growth factor therapies for central nervous system repair. Our data suggest that epidermal growth factor‐containing hydrogels can shift astrocytes into potentially beneficial A2‐like phenotypes that may augment neuroprotection and neuroplasticity during the recovery phase after brain injury.
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- 2019
29. Translational relevance of the goat as a preclinical model of the human labrum and chondrolabral junction-histological study
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Scot T. Martin, John W. Stelzer, Ravikumar Rajappa, William K. Conaway, Swetha Rokkappanavar, Shivam Upadhyaya, Hu Ping Hsu, Mark R. Nazal, Wanting Niu, Kyle Alpaugh, and Myron Spector
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0206 medical engineering ,02 engineering and technology ,Chondrocyte ,Extracellular matrix ,03 medical and health sciences ,Femoral head ,0302 clinical medicine ,Laminin ,medicine ,Animals ,Humans ,Orthopedics and Sports Medicine ,030203 arthritis & rheumatology ,Labrum ,biology ,Acetabular labrum ,Goats ,Anatomy ,020601 biomedical engineering ,medicine.anatomical_structure ,Models, Animal ,biology.protein ,Hip Joint ,Elastin ,Type I collagen ,Hip Injuries - Abstract
The purpose of this study was to evaluate the histologic features of the caprine labrum, with emphasis on the chondrolabral junction, with the goal of informing the feasibility of the goat as an animal model. The left hip joint of six adolescent Spanish goats (Capra pyrenaica) was harvested and subjected to anatomical and histological assessments. Human acetabular and femoral head samples, collected during total hip arthroplasty, served as comparison samples. The caprine labrum was found to consist of mostly type I collagen with uniform crimp, with an average crimp length of 20.8 µm. Upon histological assessment, acetabular articular chondrocytes were found to express substance-P, especially near or in the chondrolabral junction. And the majority of nonvascular cells expressed α-smooth muscle actin (SMA), with no notable elastin and laminin expression. Human labrum demonstrated similar staining patterns. Overall, the goat hip was found to be homologous to the human hip, demonstrating potential as a useful animal model for future studies. This is the first report of a crimped collagen structure in the labrum. Crimped type I collagen at the chondrolabral junction imparts an extension-recovery property which allows for toleration of stress without permanent deformation, underlying the importance of its preservation during surgery. The high expression of substance-P reflects the degree to which the labrum is innervated. Finally, the expression of α-SMA with contractile characteristics could indicate the potential for chondrocyte (i.e., myochondrocytes) modeling of the extracellular matrix. Statement of Clinical Significance: Establishment of a large animal model and deeper knowledge of the histological composition of the hip joint will enhance our study of the acetabular labrum, including repair techniques. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:1070-1080, 2020.
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- 2019
30. Extracorporeal Shock Wave Stimulates Angiogenesis and Collagen Production in Facial Soft Tissue
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Diana Heimes, Talal Alnassar, Wanting Niu, Myron Spector, Peer W. Kämmerer, and Abdulmonem Alshihri
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Male ,Pathology ,medicine.medical_specialty ,Angiogenesis ,Surgical Wound ,Neovascularization, Physiologic ,Surgical Flaps ,Microcirculation ,High-Energy Shock Waves ,03 medical and health sciences ,0302 clinical medicine ,Vascularity ,Medicine ,Animals ,Humans ,Skin ,Wound Healing ,integumentary system ,business.industry ,Goats ,Soft tissue ,Histology ,Extracorporeal shock wave ,Disease Models, Animal ,030220 oncology & carcinogenesis ,Face ,Models, Animal ,Immunohistochemistry ,Tissue healing ,030211 gastroenterology & hepatology ,Surgery ,Collagen ,medicine.symptom ,business - Abstract
Background This study investigated the efficacy of extracorporeal shock wave (ESW) application in stimulating dermal thickness, vascularity, and collagen synthesis of facial skin in a large animal model. Materials and methods The facial skin of the maxillary and mandibular areas of goats (n = 6 per group) was treated with ESWs of different intensities (0.15 and 0.45 mJ/mm2; 1000 pulses). After 4 d, histology and immunohistochemistry were used to evaluate the following: dermal thickness, total number and abundance of microvessels, amount of type 1 collagen, and α-smooth muscle actin expression. Results Dermal thickness, number and abundance of microvessels, and collagen synthesis increased after ESW application at both intensities (each P Conclusions A single-session application of focused low-energy ESWs to facial skin can increase dermal thickness by stimulating collagen production and local microcirculation. These findings commend the technique for future investigation for pretreatment of local or microvascular skin flaps to enhance tissue healing.
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- 2019
31. Supplementary_Data - In Situ Cross-linking Hydrogel as a Vehicle for Retinal Progenitor Cell Transplantation
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Jeayoung Park, Baranov, Petr, Aydin, Aybike, Abdelgawad, Hany, Singh, Deepti, Wanting Niu, Motoichi Kurisawa, Spector, Myron, and Young, Michael J.
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education ,Medicine ,Cell Biology - Abstract
Supplementary_Data for In Situ Cross-linking Hydrogel as a Vehicle for Retinal Progenitor Cell Transplantation by Jeayoung Park, Petr Baranov, Aybike Aydin, Hany Abdelgawad, Deepti Singh, Wanting Niu, Motoichi Kurisawa, Myron Spector, and Michael J. Young in Cell Transplantation
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- 2019
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32. Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis
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Jie Xie, Zixun Dai, Hua Liu, Kun Li, Pengfei Lei, Yu Shrike Zhang, Long Wang, Wanting Niu, and Yihe Hu
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lcsh:Diseases of the musculoskeletal system ,Osteolysis ,Macrophage ,chemistry.chemical_element ,Calcium ,Cell Line ,Ultrahigh molecular weight polyethylene ,03 medical and health sciences ,Mice ,0302 clinical medicine ,lcsh:Orthopedic surgery ,Western blot ,Osteogenesis ,medicine ,Animals ,Humans ,Orthopedics and Sports Medicine ,MTT assay ,Particle Size ,Fibroblast ,Wear particles ,Cell Proliferation ,030203 arthritis & rheumatology ,030222 orthopedics ,biology ,medicine.diagnostic_test ,Dose-Response Relationship, Drug ,business.industry ,Macrophages ,Fibroblasts ,medicine.disease ,Molecular biology ,lcsh:RD701-811 ,medicine.anatomical_structure ,chemistry ,Osteocalcin ,biology.protein ,Alkaline phosphatase ,Surgery ,lcsh:RC925-935 ,Polyethylenes ,business ,Macrophage proliferation ,Research Article - Abstract
Background In the ultrahigh molecular weight polyethylene (UHMWPE) prosthetic environment, fibroblasts affected by wear particles have the capacity of osteogenesis to reduce osteolysis. We aimed to assess the effects of macrophages on the osteogenic capability of fibroblasts treated with UHMWPE wear particles. Methods The effect of different concentrations of UHMWPE (0, 0.01, 0.1, and 1 mg/ml, respectively) on macrophage proliferation were validated by MTT assay to determine the optimum one. The fibroblasts viability was further determined in the co-culture system of UHMWPE particles and macrophage supernatants. The experiment was designed as seven groups: (A) fibroblasts only; (B) fibroblasts + 1 mg/ml UHMWPE particles; and (C1–C5) fibroblasts + 1/16, 1/8, 1/4, 1/2, and 1/1 supernatants of macrophage cultures stimulated by 1 mg/ml UHMWPE particles vs. fibroblast complete media, respectively. Alizarin red staining was used to detect calcium accumulation. The expression levels of osteogenic proteins were detected by Western blot and ELISA, including alkaline phosphatase (ALP) and osteocalcin (OCN). Results The concentration of 0.1 mg/ml was considered as the optimum concentration for macrophage proliferation due to the survival rate and was highest among the four concentrations. Fibroblast viability was better in the group of fibroblasts + 1/16 ratio of macrophage supernatants stimulated by 1 mg/ml of UHMWPE particles than the other groups (1:8, 1:4, 1:2, 1:1). ALP and OCN expressions were significantly decreased in the group of fibroblasts + 1/4, 1/2, and 1/1 supernatants stimulated by 1 mg/ml of UHMWPE particles compared with other groups (1/8, 1/16) and the group of fibroblasts + 1 mg/ml UHMWPE (p
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- 2018
33. Abnormal changes in voltage-gated sodium channels subtypes Na
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Gefei, Guan, Mingyi, Zhao, Xiaoxue, Xu, Tomasz, Boczek, Xiaoyuan, Mao, Zhi, Li, Qinghua, Gao, Jianing, Li, Dongyi, Zhao, Wanting, Niu, Liming, Lei, and Feng, Guo
- Subjects
Adult ,Male ,NAV1.2 Voltage-Gated Sodium Channel ,Adolescent ,Brain Neoplasms ,Voltage-Gated Sodium Channels ,Astrocytoma ,Sodium Channels ,NAV1.1 Voltage-Gated Sodium Channel ,Young Adult ,Calmodulin ,NAV1.6 Voltage-Gated Sodium Channel ,NAV1.3 Voltage-Gated Sodium Channel ,Humans ,Female ,Phosphorylation ,Calcium-Calmodulin-Dependent Protein Kinase Type 2 ,Aged ,Signal Transduction - Abstract
Epileptic seizures are the main clinical manifestation of low-grade astrocytoma. Voltage-gated sodium channels (VGSCs) play a crucial role in epilepsy. Until now, the role of VGSCs and the relationships between calmodulin (CaM)/CaM-dependent protein kinase II (CaMKII) and VGSCs in low-grade astrocytoma have not been demonstrated. In our study, the protein expression of Na
- Published
- 2018
34. Reconstruction of Large-scale Defects with a Novel Hybrid Scaffold Made from Poly(L-lactic acid)/Nanohydroxyapatite/Alendronate-loaded Chitosan Microsphere: in vitro and in vivo Studies
- Author
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Pengfei Lei, Yu Shrike Zhang, Jie Xie, Jianming Ruan, Gengyan Liu, Longbo Zhang, Hongwei Wu, Yihe Hu, Wanting Niu, Jingzhou Yang, Chaoyue Zhang, and Hua Liu
- Subjects
Scaffold ,Bone Regeneration ,Science ,02 engineering and technology ,Bone healing ,In Vitro Techniques ,Matrix (biology) ,010402 general chemistry ,01 natural sciences ,Article ,Chitosan ,chemistry.chemical_compound ,Drug Delivery Systems ,Osteogenesis ,In vivo ,Animals ,Lactic Acid ,Cells, Cultured ,Multidisciplinary ,Alendronate ,Tissue Engineering ,Tissue Scaffolds ,021001 nanoscience & nanotechnology ,Controlled release ,Microspheres ,In vitro ,0104 chemical sciences ,Durapatite ,chemistry ,Bone Substitutes ,Drug delivery ,Medicine ,Rabbits ,0210 nano-technology ,Biomedical engineering - Abstract
A chitosan-based microsphere delivery system has been fabricated for controlled release of alendronate (AL). The present study aimed to incorporate the chitosan/hydroxyapatite microspheres-loaded with AL (CH/nHA-AL) into poly(L-lactic acid)/nanohydroxyapatite (PLLA/nHA) matrix to prepare a novel microspheres-scaffold hybrid system (CM-ALs) for drug delivery and bone tissue engineering application. The characteristics of CM-ALs scaffolds containing 10% and 20% CH/nHA-AL were evaluated in vitro, including surface morphology and porosity, mechanical properties, drug release, degradation, and osteogenic differentiation. The in vivo bone repair for large segmental radius defects (1.5 cm) in a rabbit model was evaluated by radiography and histology. In vitro study showed more sustained drug release of CM-AL-containing scaffolds than these of CM/nHA-AL and PLLA/nHA/AL scaffolds, and the mechanical and degradation properties of CM-ALs (10%) scaffolds were comparable to that of PLLA/nHA control. The osteogenic differentiation of adipose-derived stem cells (ASCs) was significantly enhanced as indicated by increased alkaline phosphates (ALP) activity and calcium deposition. In vivo study further showed better performance of CM-ALs (10%) scaffolds with complete repair of large-sized bone defects within 8 weeks. A microspheres-scaffold-based release system containing AL-encapsulated chitosan microspheres was successfully fabricated in this study. Our results suggested the promising application of CM-ALs (10%) scaffolds for drug delivery and bone tissue engineering.
- Published
- 2017
35. Nanogels for biomedical applications
- Author
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Kyriakos Dalamagkas, Cang Wang, Magdalini Tsintou, Ding Weng, Yi-Nan Zhang, and Wanting Niu
- Subjects
Scaffold ,Materials science ,Biocompatibility ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Tissue engineering ,Drug delivery ,Nanocarriers ,0210 nano-technology ,Biosensor ,Biomedical engineering - Abstract
Nanogels have attracted much attention in the recent decades due to the combined properties of nanotechnology and cross-linking gel-like materials. These nanogels show great biocompatibility and biodegradability both in vitro and in vivo studies and have been widely used into biomedical science in terms of imaging, diagnostics, drug delivery, tissue engineering, biosensors, etc. The physicochemical properties (sizes, shapes, charges, and surface chemistries) can be easily tuned, which offer nanogels many advantages as nanocarrier for delivery of small molecules, proteins, genes, and other therapies. Incorporation of nanoparticles with a scaffold or hydrogel provides the ability to manipulate nanoscale parameters of scaffold for the development of 3D printing technology in the field of tissue engineering. In addition, these nanogels can sense and respond to environmental stimuli such as temperature, pH, light, mechanical stress, electric, magnetic field and glucose concentration. The special properties and simultaneously possesses characteristic of hydrogel and nanoparticle system make them potential candidates for sensory elements of biosensor.
- Published
- 2017
36. List of Contributors
- Author
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Farzaneh Aavani, Samad Ahadian, Ebru Altuntaş, Yasemin Budama-Kilinc, Rabia Cakir-Koc, Ayse Candayan, Sefa Celik, Kyriakos Dalamagkas, Kubra Gozutok, Madalina Elena Grigore, Alexandru Mihai Grumezescu, Alina Maria Holban, Mohammed Inayathullah, Serda Kecel-Gunduz, Amirsalar Khandan, Nasim Kiaie, M. Miraftab, M. Rezaa Mohammadi, Fatemeh Mohandes, Parisa Nazemi, Wanting Niu, Farideh Ordikhani, Burak Ozdemir, Aysen E. Ozel, Burcu Özkan, Olga N. Oztel, Milica Radisic, Jayakumar Rajadas, Mehdi Razavi, Anzelika Schreiber, Abdolreza Simchi, Wenchao Sun, Magdalini Tsintou, Cang Wang, Ding Weng, Frank Witte, Gülgün Yener, Serap Yesilkir-Baydar, Yi-Nan Zhang, Yu S. Zhang, and Kai Zhu
- Published
- 2017
37. PRP lysate‐infused gelatin hydrogel as a scaffold for bone reconstruction
- Author
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Meral Nadra, Wanting Niu, Motoichi Kurisawa, and Myron Spector
- Subjects
Scaffold ,Lysis ,food.ingredient ,food ,Chemistry ,Oral Surgery ,Gelatin ,Biomedical engineering - Published
- 2019
38. Preparation of Water-Soluble CdTe Quantum Dots Based Immunofluorescent Probes and Its Application in Serological Immunoassay
- Author
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Diallo Mamadon Moctar, Min Pan, Yuquan Chen, Wanting Niu, and Luyao Zhang
- Subjects
Chromatography ,medicine.diagnostic_test ,Chemistry ,medicine.drug_class ,technology, industry, and agriculture ,equipment and supplies ,Condensed Matter Physics ,Monoclonal antibody ,Fluorescence ,Electronic, Optical and Magnetic Materials ,Serology ,Nanomaterials ,Autofluorescence ,Water soluble ,Biochemistry ,Control and Systems Engineering ,Quantum dot ,Immunoassay ,Materials Chemistry ,Ceramics and Composites ,medicine ,Electrical and Electronic Engineering - Abstract
Nanomaterials are honored as new materials of the 21st century. Compared with traditional organic dyes, quantum dots (QDs) have attracted extensive research interest due to their excellent fluorescent characteristics. In this research, the mercaptopropionic acid capped QDs were linked with monoclonal antibodies via EDC/NHS as immunofluorescent probes to detect the antigens in serum. The bioactivity and the titer of antibodies in the fluorescent probes were analysed with the method of enzyme linked immunosorbent assay. At last, the feature of human serum autofluorescence was analysed and then the emission wavelength range and the concentration of QDs needed in serological immunoassay were decided.
- Published
- 2011
39. The Application of Stem Cell Based Tissue Engineering in Spinal Cord Injury Repair
- Author
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Wanting Niu and Xiang Zeng
- Subjects
Tissue engineering ,business.industry ,Mechanism (biology) ,Monoplegia ,Medicine ,Stem cell ,Bioinformatics ,Functional recovery ,business ,medicine.disease ,Paraplegia ,Spinal cord injury ,Tetraplegia - Abstract
Spinal Cord Injury (SCI) results in the permanent functional impairment, leading to monoplegia, paraplegia or tetraplegia with tremendous social and economic burden. The intrinsic repair mechanism has been proven to be insufficient. The complex pathophysiology after injury imposes enormous challenging to functional recovery, given the most advanced medical intervention nowadays. Therefore, the development of effective therapeutic strategy for spinal cord injury management is in great need. Here we review a stem cell based tissue engineering approach under preclinical or clinical development for spinal cord injury, with a focus on promoting functional recovery after SCI, aiming to provide some beneficial suggestion on stem cell based tissue engineering design.
- Published
- 2015
40. Osteogenic application of injectable gelatin-hyaluronan matrices: In vitro study with bone marrow mesenchymal stem cells
- Author
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Wanting, Niu, primary, Ding, Weng, additional, Abdulmonem, Alshihri, additional, Teck Chuan, Lim, additional, Li-Shan, Wang, additional, Motoichi, Kurisawa, additional, and Myron, Spector, additional
- Published
- 2016
- Full Text
- View/download PDF
41. Astrocyte migration in injectable gelatin-hydroxyphenyl propionic acid matrices for neuronal guidance in spinal cord injury
- Author
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Josna, Joseph, primary, Kazuhide, Hayakawa, additional, Motoichi, Kurisawa, additional, Li Shan, Wang, additional, Wanting, Niu, additional, and Myron, Spector, additional
- Published
- 2016
- Full Text
- View/download PDF
42. Nanoparticle drug delivery systems and three-dimensional cell cultures in cancer treatments and research
- Author
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Wanting Niu, Ding Weng, and Wenjin Shi
- Subjects
Nanoparticle ,02 engineering and technology ,010402 general chemistry ,lcsh:RC254-282 ,01 natural sciences ,In vitro model ,Human health ,three-dimensional cell cultures ,Medicine ,Cancer research and treatments ,tissue engineering techniques ,Tumor microenvironment ,nanoparticle drug delivery systems ,business.industry ,Cancer ,General Medicine ,Mesoporous silica ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,021001 nanoscience & nanotechnology ,medicine.disease ,0104 chemical sciences ,Biotechnology ,Cell culture ,Drug delivery ,Cancer research ,0210 nano-technology ,business - Abstract
Being a great threat to human health, with no permanent cure yet, better treatment and further research in cancer are inevitable. Nanoparticle drug delivery systems (NDDSs), especially pH-sensitive NDDSs, such as lipid-based, polymeric, and mesoporous silica nanoparticles have played a significant role in cancer treatments. Further, three-dimensional (3D) cell cultures models, which include tumor spheroid models, microfluidic systems, and matrix/scaffolds-based 3D tumor, better mimic the tumor microenvironment than the conventional two-dimensional cultures, making it possible to better understand the disease while serving as a useful in vitro model for future research. The present review mainly focuses on such 3D cell cultures and drug delivery systems that are applied in cancer research and treatments.
- Published
- 2016
43. [Application of quantum dots in biomedical detection]
- Author
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Luyao, Zhang, Wanting, Niu, Hao, Yang, and Min, Pan
- Subjects
Quantum Dots ,Biomarkers, Tumor ,Fluorescence Resonance Energy Transfer ,Protein Array Analysis ,Animals ,Humans ,Sensitivity and Specificity - Abstract
Semiconductor quantum dots (QDs) are a new kind of biological fluorescence material, which has many advantages, such as broad excitation spectra, tunable emission spectra and good photostability. In the field of biomedical detection, the problems encountered in the traditional organic dye-based biomedical detections, such as short fluorescence lifetime and failure to simultaneous excitation of multiple colors, can be solved by using QDs. Water-soluble QDs combined with specific bio-molecules can label targeting bio-compound, which is useful in bio-molecule detection, cell labeling, tissue imaging, and can be used in fluorescence resonance energy transfer (FRET) technology. Combining QDs and protein chip technology to develop a new technology to detect multiple kinds of tumor markers will be one of the promising clinical applications of QDs with greater sensitivity, specificity, rapidity and convenience.
- Published
- 2011
44. [Research advances of techniques for quantum dots-based tumor marker detection]
- Author
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Wanting, Niu, Renhui, Wang, Hao, Yang, Min, Pan, and Yuquan, Chen
- Subjects
Quantum Dots ,Biomarkers, Tumor ,Humans - Abstract
Quantum dots, as a new kind of biological fluorescence material, have properties superior to the traditional organic dyes. In the recent decade, researches about the application of quantum dots in biomedicine have made great progress. Tumor markers have vital importance in the diagnosis and treatment of carcinoma; however, they are not widely used for lack of specificity and sensitivity. Researches about applications of quantum dots in the detection of tumor markers are centralized in three aspects, namely the detection of serum/plasma tumor markers, the detection of tissue and cell tumor markers (such as molecular imaging of cell and tissue), and the detection of tumor in vivo animals. A combination of quantum dots and flow cytometry technique for developing a new technique to detect multiple kinds of tumor markers at the same time with greater sensitivity, specificity, rapidity and convenience may have the potential for clinical use of quantum dots.
- Published
- 2010
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