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Partially stabilized zirconia （7YSZ） with a mass fraction of 7±1% yttrium oxide is a widely used ceramic material for thermal barrier coatings. However， its phase stability， sintering resistance， and mechanical properties decreased during the long-time operation above 1200 ℃. A new type of Sc2O3-Y2O3 co-doped ZrO2 thermal barrier coating ceramic material was proposed ， and a molar fraction of 7.5%Sc2O3-x%Y2O3-（92.5-x）%ZrO2（x=0，0.1，0.2，0.3） ceramics were prepared by solid-state reaction method. The effects of Y2O3 doping on the microstructural， phase evolution， and mechanical properties（including Vickers hardness， fracture toughness， elastic modulus and three-point bending strength）were explored by XRD， SEM， and other testing methods. The results show that the relative density of Sc2O3-Y2O3 doped ZrO2 ceramics sintered at 1450 °C for 3.5 h is more than 97%， and the phase structure is composed of tetragonal phase. Compared with 6-8YSZ ceramics， Sc2O3-Y2O3 doped ZrO2 ceramics exhibit similar Vickers hardness （13-14 GPa）， fracture toughness （6.5-7.0 MPa·m1/2）， elastic modulus （211-214 GPa）and three-point bending strength（520-850 MPa）. Fracture mechanism shows a mixture of transgranular and intergranular fracture modes， in which transgranular fracture is dominant.This ceramic can be explored as a potential thermal barrier coating material for high-temperature applications.

The variable camber trailing edge enables the aircraft to maintain the best aerodynamic performance throughout the flight envelope, so as to achieve the ultimate goal of reducing aircraft fuel consumption and air pollutant emissions. It is one of the important characteristics and development direction of the new generation civil aircraft. In order to solve the contradiction between the high load-bearing and the large deformation of the variable camber trailing edge of large aircraft, a structure scheme for variable camber trailing edge based on multi-block rotation was proposed. A parametric optimization method was established for smooth and continuous deformation of multi-block rotating mechanism. The multi-block rotating structure and driving system of variable camber trailing edge were designed, and the deformation function of the variable camber trailing edge demonstrator was preliminarily verified by ground test. The result shows that variable camber wing trailing edge designed by using the present optimization method of multi-block rotating mechanism can realize smooth and continuous morphing. The actual deformation range of the demonstration is 3.9 degrees up to 12.5 degrees down, and the error with the design target is 16.7%. It provides a design reference for solving the engineering application problem of variable camber structure for large aircraft.

The new version of the gear accuracy standard GB/T 10095.1—2022 has been released, and the standard of accuracy of rack is written with reference to the gear accuracy standard. The manufacturing technology and the processing accuracy of racks have changed greatly, so the standard of accuracy of rack has been revised. The main differences between the 2022 version of the standard of accuracy of rack and the 1988 version are: terms and symbols; definition of total tooth profile deviation; measurement of the rack pair; adding the parts detection method of flame cutting large modulus racks for lifting and lowering of offshore platforms, as well as five measurement tools that can be used in the production process of racks. Analysis is conducted concerning the added and the revised parts.

The second edition of ISO 1328-2 was released in 2020, which adjusted the scope of application and modified the evaluation system of accuracy level to distinguish it from ISO 1328-1. In order to better understand and apply the gear accuracy standard, the author starts with the change of the standard name and calculation rules of the deviation allowable values, and analyzes the relationship between the differences of each deviation item and the dependent variables in the first and second editions of ISO 1328-1 with the help of the modulus mn, the diameter d of the dividing circle and other variables. At the same time, the author analyzes and compares the new and old editions of ISO 1328-2 in the scope of application, tolerance classification and deviation items and differences in the determination methods of allowable deviation values. The results show that the first and second editions are quite different and should be paid attention to.

In view of the lack of design theory and load-bearing calculation method of dual-lead precision worm drives, the meshing geometry model of the dual-lead worm drive is established based on the principle of spatial meshing, and the design methods of geometric parameters, adjustment and compensation of this worm drive are proposed. Based on the ISO/TS 14521 and using the relative calculation method, loading capacity calculation models for the transmission efficiency, tooth surface wear, tooth surface pitting and tooth root broken are developed. The dual-lead precision worm drive prototype is designed and manufactured, and the transmission performance test is carried out. Through the theoretical analysis, simulation calculation and prototype performance experiment, the correctness of the design method and the reliability of the load-bearing calculation method are verified, which provides a theoretical basis for the design and analysis of the wide application of dual-lead precision worm drives.

High temperatures cause physiological and biochemical changes and significantly affect young panicle development of rice (Oryza sativa L.). Brassinosteroids play important roles in enhancing crop stress resistance. In this study, we subjected rice cultivars Huanghuazhan (heat-resistant) and IR36 (heat-sensitive) to high temperature (HT, 40 ºC) or normal temperature (NT, 33 ºC) for 7 d at the panicle initiation stage, in conjunction with application of 24-epibrassinolide [EBR, a synthetic brassinolide (BR)] or brassinazole (BRZ, a BR biosynthesis inhibitor) at the beginning of the treatments. HT exacerbated spikelet degeneration and inhibited young panicle growth, which were partially prevented by EBR application, while BRZ application aggravated the reduction in spikelet number. HT decreased the contents of BR, active cytokinins (aCTK), active gibberellins (aGA) and indole-3-acetic acid (IAA), but increased the content of abscisic acid (ABA) in young panicles. The activities of key enzymes involved in sucrose hydrolysis, glycolysis and the tricarboxylic acid cycle in young panicles were decreased with the change of endogenous hormone levels under HT. In addition, the contents of H2O2 and malondialdehyde (MDA) were increased and the activities of antioxidant enzymes were decreased in young panicles. Exogenous application of EBR induced the expression of phytohormone biosynthesis-related genes and down-regulated the expression of phytohormone catabolism-related genes to increase the contents of endogenous BR, aCTK, aGA and ABA, thus promoting the decomposition and utilization of sucrose in young panicles, enhancing the activities of superoxide dismutase, catalase and peroxidase, and reducing the accumulation of H2O2 and MDA in young panicles, whereas application of BRZ had the opposite physiological effects. These results showed that brassinosteroids mediate endogenous phytohormone metabolism to alleviate HT injury at the panicle initiation stage in rice.

Cs2LiLaBr6: Ce (CLLB) scintillator with the size of Φ 21mm × 25 mm coupled with PMT was used to detect neutron and gamma rays. The pulse shape discrimination (PSD) of neutrons and gamma rays by charge comparison method, the neutrons and gamma rays from AmBe source and fast neutron beam can be separated with figure-of-merit (FOM) values of 0.9 and 1.3, respectively. However, some neutron and gamma rays are difficult to distinguish, so new algorithms need to be investigated to improve the PSD performance of neutron and gamma. Artificial neural networks (ANN) have a very good image recognition capability, thus the ANN model was constructed to discriminate the waveforms of neutron and gamma rays. After ANN model training, the neutron and gamma signals of the CLLB detector were recognized with an accuracy of 98%, and the FOM value of the ANN method was calculated to be 19.4. This result is much higher than the charge comparison method, indicating better discrimination between neutrons and gamma rays with the ANN method.

To make sure the half-bridge three-level dual active bridge (HBTL-DAB) circuit works correctly,the problem of capacitor voltage equalization between the upper and lower direct current (DC) buses on the three-level side must be solved. In the traditional symmetrical control mode,the capacitor voltage equalization control under load can be achieved by fine-tuning the duty cycle. However,in the case of no-load,the symmetrical control mode is difficult to realize the balance adjustment of the upper and lower DC bus capacitor voltages from the control strategy. Therefore,in view of the above problem,an asymmetric control method for the HBTL-DAB circuit is proposed. Firstly,the reason why the symmetrical mode cannot be equalized is theoretically analyzed. And then according to the asymmetric method,energy deviation on the upper and lower DC bus capacitor between positive and negative half cycles under no-load conditions is calculated. The capacitor voltage equalization control is achieved by adjusting the energy deviation,and the main factors affecting the effect of voltage balance control are also pointed out. Finally,the asymmetric control method is verified under various working conditions by simulation. The results show that no matter in condition of existing external continuous unbalance factors,or external initial unbalance factors,or internal pulse error factors,voltage balance adjustment is achieved by the asymmetric control method under no-load and loaded conditions.

Objective To investigate the effect of circular RNA hsa_circ_0001922 on the proliferation, migration and invasion of prostate cancer cell PC-3 and its underlying molecular mechanism. Methods qRT-PCR and RNA FISH were used to detect the expression level and localization of hsa_circ_0001922 in PC-3 cells respectively. After hsa_circ_0001922 was targeted inhibited, clone formation, Transwell assay and scratch assay were used to detect the proliferation, migration and invasion abilities of PC-3 cells. qRT-PCR and Western blot were used to detect the expression levels of EMT pathway-related molecules after inhibiting hsa_circ_0001922. Results The expression of circular RNA hsa_circ_0001922 was increased in PC-3 cells (P < 0.01) and it existed in the cytoplasm and nucleus. The invasion, migration and invasion abilities were significantly weakened (P < 0.05) after inhibiting hsa_circ_0001922; the expression of E-cadherin mRNA increased (P < 0.05) while the mRNA level of Vimentin decreased (P < 0.05). The results of Western blot were consistent with the above (both P < 0.05). Conclusion The circular RNA hsa_circ_0001922 may promote the proliferation, migration and invasion of PC-3 cells through the EMT pathway.

Circular RNAs contribute to the progression of glioma. However, the biological role and underlying mechanism of circ_0082375 in glioma remain unclear. Quantitative real-time PCR and Western blot assay were used to evaluate the expression levels of circ_0082375, microRNA-485-5p, and Wnt family member 7B (Wnt7B). The overall survival of glioma patients was estimated by the Kaplan–Meier curve. Cell proliferation, apoptosis, invasion, and migration were detected by cell counting kit-8, 5-ethynyl-2 -deoxyuridine (EdU) staining, flow cytometry, and transwell assays, respectively. Glucose level and lactate production were determined using glucose and lactate assay kits. In vitro angiogenesis assay was used to evaluate the angiogenesis of glioma cells. The interaction between microRNA (miR)-485-5p and circ_0082375 or Wnt family member 7B (Wnt7B) was verified by dual-luciferase reporter and RNA immunoprecipitation assays. A xenograft model was used to verify the function of circ_0082375 in vivo. circ_0082375 was upregulated in glioma tissues, and it was closely related to the prognosis of glioma patients. circ_0082375 knockdown suppressed cell proliferation, migration, invasion, angiogenesis, glycolysis, and epithelial-mesenchymal transition (EMT), and promoted cell apoptosis in glioma cells. irc_0082375 was a sponge of miR-485-5p, which directly targeted Wnt7B. Knockdown of circ_0082375 inhibited the malignancy, angiogenesis, and glycolysis of glioma cells in vitro by sponging miR-485-5p. Besides, circ_0082375 knockdown hampered the growth of glioma growth by regulating the miR-485-5p/Wnt7B axis in vivo. Altogether, circ_0082375 regulated miR-485-5p/Wnt7B axis to promote the malignancy, angiogenesis, and glycolysis of glioma cells, thereby contributing to the progression of glioma.

The main goal of this study is to explore the drivers of meaningful sport consumption and its influence mechanism. In sports consumption, consumers not only seek hedonic value but also pursue to experience greater purpose and meaning in life, which is regarded as meaningful sports consumption. This study extends existing sports management literature by examining how social needs impact meaningful sports behavior with team affiliation, self-improvement, and self-esteem as mediators. Based on the questionnaire data collected from China, the empirical analysis results show that social needs have a significant positive impact on meaningful sports consumption behavior through the mediating effect of team affiliation and self-esteem motivation. However, self-improvement motivation does not have a mediating effect on the relationship between social needs and meaningful sports consumption. This study enriches the research content of sports consumption, adds research object of social needs, and expands the research scope of meaningful consumption by introducing meaningful sports consumption into the above domain.

Objective To analyze the learning curve experience of hip arthroscopy based on patient demographics, surgical time, portal setup time, and postoperative complications and to find the key point in the learning curve. Methods From May 2016 to February 2019, a prospective study on the learning curve experience of hip arthroscopy was performed in our hospital. We evaluated the first 50 consecutive hip arthroscopy procedures performed by a single surgeon. There were nine females and 41 males with a mean age of 30.8 years. We divide the patients into early group and late group according to the date of their operation, with each group including 25 patients. Data on patient demographics, types of procedure, surgical time, portal setup time, and postoperative complications were collected. Functional scores were assessed with the modified Harris Hip Score (mHHS). Results Patients were followed up for 16.4 months on average (range, 13–27 months). The early group of patients had a mean age of 35.2 years and the late group a mean age of 26.5 years. The most common procedures performed for the early group were debridement (17 patients, 68%), and in the late group, most patients underwent labral repair (18 patients, 72%). Mean total surgical time was 168 min for the early group and 143 min for the late group, and there was no statistically significant difference between two groups. The portal setup time in the early group and late group was 40.2 ± 12.4 min and 18.5 ± 6.2 min, respectively (P

Objective To prepare the tumor targeting multifunctional composite nanoparticles (DIPP-NPs) by encapsulating sinoporphyrin sodium (DVDMS), IR780 and perfluorinated hexane (PFH), evaluate their physicochemical properties, and explore the synergistic enhancement of sonodynamic therapy (SDT) between DVDMS and IR780. Objective DIPP-NPs were synthesized via double emulsion and then characterized. The changes of nanoparticles before and after ultrasonic irradiation were observed by optical microscopy. Ultrasonic and photoacoustic imaging systems were used to evaluate the imaging capability in vitro. Targeting ability in vitro was evaluated by confocal laser scanning microscopy. The production of reactive oxygen species (ROS) was demonstrated by singlet oxygen detection (SOSG). CCK-8 assay was used to detect the cytotoxicity and synergistic enhancement of SDT in murine mammary carcinoma cell line 4T1. The synergistic enhancement of SDT in 4T1 xenograft mouse model was evaluated by measuring tumor volume and weight to calculate the inhibitory rates. Results DIPP-NPs were successfully prepared, as uniform round-shaped particles, 341.17±16.97 nm in diameter and -12.77±0.88 mV in Zeta potential. The encapsulation rate of DVDMS and IR780 was (92.84±1.58) % and (96.57±1.48) %, respectively. After irradiated with ultrasonic, DIPP-NPs were getting bigger and bigger in diameter under a optical microscope. Meanwhile, ultrasound imaging results showed that the ultrasound signals were enhanced with prolonged irradiation time. In addition, photoacoustic imaging results showed that the photoacoustic signal intensity was increased with the increment of nanoparticle concentration. Laser confocal microscopy demonstrated that there were more nanoparticles around 4T1 cells in DPP-NPs, IPP-NPs and DIPP-NPs groups than in PP-NPs group. SOSG detection results showed that the production of ROS was gradually increased with prolonged irradiation time. CCK-8 assay indicated that there was no obvious cytotoxicity of the nanoparticles and the cell viability in DIPP-NPS+US group was (56.04±2.28)%, which was obviously lower than that in DPP-NPS+US [(76.83±1.52)%] and IPP-NPS +US groups [(74.03±2.64)%, both P < 0.05]. Xenograft experiment showed that the inhibitory rate was (73.28±.58)% for tumor volume, and (75.76±3.49)% in weight in DIPP-NPS+US group, which were significantly higher than those in DPP-NPS+US [(46.93±4.41)%, (43.50±2.27)%] and the IPP-NPS+US groups [(47.44±1.27)%, (44.87±4.30)%, P < 0.05]. Conclusion The tumor-targeting multifunctional composite nanoparticles DIPP-NPs are successfully prepared, which have a good synergistic enhancement of SDT effectiveness.

This study investigated the role of social capital in the relationship between sports event volunteer management and volunteers’ attitude and behavior. Social capital was discovered to mediate the relationships of sports event volunteer management with volunteers’ satisfaction and work engagement. These relationships were empirically examined by conducting a survey of 303 volunteers from the 2016 Wuhan Open and 2016 Wuhan Marathon. The results revealed that sports event management practices, namely training and working conditions, significantly affected the volunteers’ social capital. The social capital of the sports event volunteers significantly affected their satisfaction and work engagement. The volunteers’ social capital promoted their satisfaction and engagement only if sports event volunteer management practices effectively enhanced their social capital. This study provides recommendations to help sports event organizers motivate volunteers.

Color is a vital characteristic of soil. It is also an important index to reveal the sedimentary environment and climate change of soil in a certain period. A quantitative description of color by the CIELAB color system can effectively avoid the significant influence of the human visual system on color perception. Additionally, it can be more intuitively used in the study of stratigraphic correlation, mineral content, and environmental change, and can help in explaining the changes in material sources and paleoclimate environment and revealing the remarkable characteristics of climate change during periodic glacial and interglacial periods. There are several loess-paleosol sedimentary sequences in the north of the Shandong Peninsula that contain abundant paleoenvironmental information. This study considered a typical section in the Zhifu District of Yantai City as a research object to explore the climate change characteristics of the northern Shandong Peninsula during specific periods. A chronological framework was built by collecting samples, dividing strata, and optically-stimulated luminescence dating, Moreover, the chromaticity was measured, the characteristics of chromaticity changing with section depth was analyzed, and the climate characteristics and change law of the last interglacial period was discussed. The results showed that 1) the bottom age of the profile was 124.9 ka, while the top age was approximately 62.85 ka, indicating that the main body belongs to the last interglacial deposit. The stratigraphic age-depth framework was constructed based on the piecewise sedimentation rate interpolation method, with a significant correlation between age and depth. This indicates that the new and old relationships between stratigraphic age and sequence are consistent, with no sedimentary fault or stratigraphic inversion. The chromaticity parameter results showed that the average value of L* was 52.13, which is higher in the loess layer than that of the paleosol layer; its high value indicated cold and dry climate conditions. The average values of a*, b*, C*, and h* were 11.18, 25.03, 27.43, and 0.421, respectively, indicating that the paleosol layer is higher than the loess layer; its high value indicated warm and humid climate conditions. A correlation analysis was carried out on the chromaticity parameters, indicating a significant correlation between L* and a* and between a* and b*, where a* and b* are controlled by the same substance and are the main factors affecting L*. 2) The following results were obtained by comparing the color saturation, hue angle, magnetic susceptibility of Chaona loess, and deep-sea oxygen isotopes. The chromaticity parameters showed three cycles of alternating peaks and valleys in the vertical direction of the profile. Their variation trend reflected the climate change characteristics of the last interglacial period, which was dominated by a summer monsoon, with a warm and humid climate, and more precipitation (MIS5e: 118.5‒124.9 ka). Subsequently, the intensity of the summer monsoon wind weakened, the intensity of the winter monsoon wind increased, and the air temperature dropped (MIS5d: 105.2‒118.5 ka), with the climate becoming hot and humid (MIS5c: 94.7‒105.2 ka). The monsoon intensity increased, and the temperature dropped (MIS5b: 83.4‒94.7 ka), becoming warm and humid (MIS5a: 70.18‒83.4 ka) and finally dry and cold (MIS 4: 62.85‒70.18 ka).

Objective To prepare CREKA peptide-modified docetaxel (DTX) loaded liposomes (CREKA-lipo-DTX) and to evaluate their targeted binding ability and anti-proliferative activity in vitro. Methods Firstly, Michael addition reaction was used to synthesize the targeted part DSPE-PEG2000-CREKA, and then CREKA-lipo-DTX was prepared by thin film hydration-ultrasound method, and its basic characterization was evaluated. Both PC-3 cells and RWPE-1 cells were divided into 3 groups respectively (n=3): blank control group, PEG-lipo-DiI group and CREKA-lipo-DiI group. After co-incubation with the cells, the cellular uptake of liposomes was analyzed qualitatively and quantitatively by laser confocal microscopy and flow cytometry respectively, so as to evaluate the targeting effect of CREKA modified vector on PC-3 cells. The cellular uptake of liposomes was analyzed qualitatively and quantitatively by laser confocal microscopy and flow cytometry respectively. The anti-proliferative activity of CREKA-lipo-DTX was evaluated by CCK-8 assay and Annexin V-PI apoptosis detection kit on blank control group, free DTX group, PEG-lipo-DiI group and CREKA-PEG-DiI group of PC-3 cells. Results CREKA-lipo-DTX was successfully prepared, with an average particle size of 130.37±1.44 nm, and encapsulation rate of (91.93±4.64)%. The obtained drug carrier had good stability and showed good slow-release behavior in vitro. The results of laser confocal microscopy and flow cytometry analysis indicated that the uptake of CREKA-modified liposomes by PC-3 cells was significantly increased (P < 0.05) when compared with the unmodified liposome group (PEG-lipo-DiI), while no such difference was seen in RWPE-1 cells (P>0.05). In vitro anti-tumor experimental results suggested that enhanced killing effect of DTX on PC-3 cells and significantly increased numbers of early and late apoptotic PC-3 cells were observed in the CREKA-lipo-DTX group when compared with the free DTX and PEG-lipo -DTX groups (P < 0.05). Conclusion A high-efficiency and low-toxicity lipoid drug delivery system with long cycle and tumor specific targeting characteristics has been successfully prepared, which can specifically target PC-3 cells in vitro, and thus can improve the anti-tumor effect of the carrier in vitro.

Objective To prepare ultrasound-guided intratumor injection of liquid-solid phase-transition biomagnetic material (NdFeB/Fe3O4/PLGA, NFP) and investigate its feasibility and efficiency of aggregating for targeted aggregation of intravenously injected magnetic liposome nanoparticles (MLPs). Methods The biomagnetic material NFP was prepared and its liquid-solid phase transition ability, morphology and magnetization performances were assessed; the form, particle size and diameter of MLPs were also characterized. The biocompatibility of NFP and MLPs were evaluated by cytotoxic proliferation experiments, and the efficiency of MLP capture by NFP was determined using an in vitro model of extracorporeal circulation. A 4T1 mouse model bearing breast cancer xenograft was established, in which NFP was injected in the tumor under ultrasound guidance and MLPs conjugated with a fluorescent dye was injected intravenously by tail vein. The efficiency of NFP implant for capturing the MLPs for targeted aggregation of the latter in the xenograft was evaluated using in vivo fluorescence imaging. Results The biomagnetic material NFP capable of liquid-solid phase transition was successfully prepared. Scanning electron microscopy and elemental mapping results showed a homogeneous distribution of the elements in NFP after phase transition. The NFP implant could be efficiently magnetized in vitro, and the magnetic forces of NFP was positively correlated with its volume (350.14±23.89, 493.47±19.54, 679.39±34.23, and 812.36±27.99 Gs for 25, 50, 75, and 100 μL of NFP, respectively). The prepared MBLs had a uniform particle size of 373.658±50.000 nm with a zeta potential of -24.8±2.5 mV. Cytotoxicity experiments with tumor cells and normal cells showed no obvious cytotoxicity of either NFP or MLPs. in vivo fluorescence imaging showed that following intravenous MLB injection and intratumor NFP implantation, the fluorescence intensity was significantly enhanced in the xenografts in the tumor-bearing mice. Conclusion The prepared liquid-solid phase-change biomagnetic NFP and the MLPs have good biocompatibility. NFP implantation in the tumor can efficiently capture intravenously injected MLPs to achieve targeted aggregation of MLPs in the tumors.

Objective To screen the possible risk factors and protective factors for prostate cancer early (in 2 years)progressing to castration resistant prostate cancer (CRPC). Methods A total of 71 patients who had progressed to CRPC in 2 years were classified as case group and 71 patients without progressing to CRPC in 2 years were classified as control group. Logistic regression analysis and survival analysis were applied to screen the risk or protective factors. Results PSA nadir and Time to PSA nadir (TTN) were statistically significant between case group and control group in logistic regression analysis (P < 0.05). Median CRPC occurrence time was 8.29 and 16 months in TTN≤9 months group and TTN > 9 months group, respectively. Median CRPC occurrence time was 12 and 9 months in PSA nadir≤0.2 ng/ml group and PSA nadir > 0.2 ng/ml group, respectively. Conclusion TTN may be a protective factor and PSA nadir may be an independent risk factor for prostate cancer early progressing to CRPC. TTN≤9 months and PSA nadir > 0.2 ng/ml are the risk factors of CRPC.

Objective To prepare a novel nanoparticle carrying iron(Ⅱ) phthalocyanine (FePc) coupled with aptamer AS1411 for targeting human breast cancer MCF-7 cells and evaluate its physicochemical properties, targeting ability to MCF-7 cells, photoacoustic/ultrasonic multimodal imaging capabilities, photothermal performance, photoinduced phase transitions in vitro and multimodal imaging performance in vivo. Methods FePc-encapsulated non-targeted nanoparticles (PLGA@FePc@PFP) were prepared by a two-step phacoemulsification method, and the targeted nanoparticles (AS1411-PLGA@FePc@PFP NPs) were prepared by combining the aptamer AS1411 modified with NH2 and the PLGA@FePc@PFP nanoparticles using the carbodiimide method. The physicochemical properties of AS1411-PLGA@FePc@PFP NPs were detected, and laser confocal microscopy and flow cytometry were used to evaluate the binding between AS1411 and the nanoparticles. CCK-8 assay was used to detect the cytotoxicity of AS1411-PLGA@FePc@PFP; laser confocal microscopy was used to observe the in vitro targeting ability of the nanoparticles; photoacoustic and ultrasonic imagers were used to test their imaging performance and targeting ability both in vitro and in vivo. Near-infrared laser (660 nm) and thermal imagers were used to test the in vitro photothermal conversion abilities of the nanoparticles, and the changes of the nanoparticles after laser irradiation were observed under an optical microscope. The temperature change of AS1411-PLGA@FePc@ PFP in tumor-bearing nude mice was detected in response to 660 nm laser irradiation. Results AS1411-PLGA@FePc@PFP was successfully prepared, which had an average diameter of 208.03±8.96 nm with a narrow particle size distribution, a mean zeta potential of -10.8±1.14 mV, and a FePc encapsulation rate of (83.96±1.61)%. The results of CCK-8 assay showed that the nanoparticles at varying concentrations had no obvious cytotoxicity. Flow cytometry showed an efficient binding of AS1411 with the nanoparticles had a binding rate of (93.31±2.58)%. Laser confocal analysis showed that there were significantly more nanoparticles around MCF-7 cells in the targeted group than in the non-targeted group. Laser irradiation caused the temperature of the nanoparticles carrying FePc to increase rapidly with time, and the phase change of the nanoparticles was observed under an optical microscope, suggesting good photothermal conversion ability of the nanoparticles. Photoacoustic imaging results showed that compared with nanoparticles without FePc, AS1411-PLGA@FePc@PFP produced strong photoacoustic signals, and the signal intensity showed a positive linear correlation with the nanoparticle concentration. Ultrasound and imaging results showed FePc in AS1411-PLGA@FePc@PFP achieved good photothermal conversion in a concentration-dependent manner for enhancing the effect of ultrasound imaging. In the tumor-bearing nude mice, the photoacoustic signal values in the xenograft were 1.26±0.06 and 0.30±0.01 at 6 h after tail vein injection of AS1411-PLGA@FePc@PFP and PLGA@FePc@PFP, respectively, showing a significant difference in their signal intensity (P < 0.05); in vivo ultrasound/contrast imaging also demonstrated a significant difference in the signal value after laser irradiation between the targeted group and the non-targeted group (P < 0.05). After laser irradiation, the tumor temperature in the targeted nanoparticle group was 50.53±0.56 ℃, as compared with 40.63±0.78 ℃ in the non-targeted group. Conclusion A multimodal molecular probe AS1411-PLGA@FePc@PFP for breast cancer has been successfully prepared, which has a good photothermal effect and can be potentially used for photoacoustic/ultrasound multimodal imaging of breast cancer.

Objective To analyze the diagnostic value of combination of transcranial ultrasound (TCS) found substantia nigra hyperechogenicity (SN+) and susceptibility weighted imaging (SWI) found loss of "swallow tail sign" (LSTS+) to diagnose parkinsonism, and assess the clinical value of TCS and SWI in the diagnosis of Parkinson's disease (PD). Methods A total of 116 patients with parkinsonism admitted the Chongqing People's Hospital from July 2016 to December 2018 were recruited in this study. And 62 of them were in the PD group and 54 in the non-PD group. The image features of TCS for nigra and SWI were analyzed in these parkinsonism patients. With SN+ found by TCS and LSTS+ found by SWI as indicators, receiver operating characteristic (ROC) curve was used to analyze the sensitivity, specificity, and area under the curve (AUC) of the 2 examinations alone or combined together in diagnosis of PD. Results There were more patients with SN+ by TCS or LSTS+ by SWI in the PD patients than the non-PD patients. The sensitivity, specificity and AUC of diagnosis of by using SN+ and LSTS+ to diagnose PD were 80.6%, 66.7%, 0.737 and 88.7%, 64.8%, 0.768, respectively. However, the sensitivity and specificity and AUC of their combination for the diagnosis were 71.0%, 88.9%, and 0.809. Conclusion In patients with parkinsonism, the results of TCS and SWI have similar efficacy in the diagnosis of PD, but TCS is better in economics and convenience. Although the sensitivity is decreased when the 2 examinations combine together, the specificity is greatly improved.

Objective To prepare the new NGR-targeted shRNA-loaded liposome, and investigate its suppressing effect on orthotopic C6 glioma in rats. Methods (1) Liposome(lipo), the NGR-targeted liposome (lipo-NGR) and shBirc5-loaded lipo-NGR were prepared successively, and all kinds of products were characterized accordingly; (2) The targeting property of shBirc5-lipo-NGR was researched by immunohistochemical method in orthotopic C6 glioma rats model; (3) The tumor growth in treated rats was monitored longitudinally with MRI T2, and the survival time of each rat was recorded simultaneously. Results (1) We successfully prepared shBirc5-lipo-NGR whose size and zeta potential were (186.2±74.07)nm and (10.9±3.76)mV; (2) The Birc5 protein was significantly decreased in shBirc5-lipo-NGR group, compared with shBirc5-lipo group, shControl-lipo-NGR group and control group; (3) The control group exhibited an average tumor volume and growth rate 4.7 times greater than that in the shBirc5-lipo-NGR group on the 12th, 17th and 22th day(P < 0.05). The median survival times of the shBirc5-lipo-NGR, shBirc5-lipo, shControl-lipo-NGR and control groups were 30, 24, 22, 21 days, respectively. Conclusion We successfully prepare the new NGR-targeted shRNA-loaded liposome, and demonstrate its targeting property, obvious inhibitory effect on tumor growth and extending the survival time of orthotopic C6 glioma-bearing rats.

The dynamics of eroded and retained soil organic carbon (SOC) may provide critical clues for evaluating impacts of soil erosion on global carbon cycling. Distribution patterns of soil aggregates in eroded and deposited environments are shaped by selective transport of water erosion. Therefore, detecting the pattern of SOC mineralization in soils dominated by aggregates of different sizes is essential to accurately explore the dynamics of eroded and retained SOCs in eroded and deposited environments. In the present study, the characteristics of SOC mineralization and its relationship to microbial dynamics in subtropical red soils dominated by different sizes of soil aggregates were investigated. The results demonstrated that the SOC mineralization rate of soils dominated by graded aggregates were significantly different, indicating that SOC mineralization in eroded and deposited environments are shaped by selective transport of water erosion. The highest mineralization rate was found in soils containing 1-2 mm aggregates at the initial stage of the experiment, and the daily average mineralization rate of the < 0.5 mm aggregates was significantly higher than that of the 2-3 mm aggregates. During the incubation, fungal communities exhibited a low dynamic character, whereas the composition of bacterial communities in all treatments changed significantly and had obvious differences relative to each other. Bacterial species diversities and relative abundances in the

Lung cancer is a malignant tumor leading to the most cancer-related deaths worldwide. The treatment efficiency of lung cancer remains poor mainly due to chemotherapy drug resistance, including cisplatin. MicroRNAs (miRNAs) are closely related to chemotherapy resistance of tumor cells. Here, we illustrated the underlying mechanism of miR-1236-3p on the DDP resistance in lung cancer cells. In this study, we found that the expression level of miR-1236-3p was significantly decreased in lung cancer tissues and A549 cell line. In addition, the half maximal inhibitory concentration (IC50) of DDP in A549 cells was significantly lower than that in A549/DDP cells, while the expression level of miR-1236-3p was prominently down-regulated in A549/DDP cells. Combining the online tool TargetScan and a dual-luciferase reporter assay, tumor protein, translationally-controlled 1 (TPT1) was proved to be the direct target gene of miR-1236-3p. The MTT and flow cytometry assays demonstrated that up-regulation of miR-1236-3p could markedly inhibit A549/DDP cell proliferation but promote apoptosis, which could be significantly reversed by pcDNA3.1-TPT1 plasmids. Finally, we further demonstrated that miR-1235-3p could restrain the expression levels of TPT1, Pim-3, phosphate-Bcl-2-associated death promoter (p-BAD) and B-cell lymphoma-extra large (Bcl-XL) in A549/DDP cells, while the inhibition could be reversed by pcDNA3.1-TPT1 as well. In a word, our study demonstrated that miR-1236-3p could reverse DDP resistance by modulation of TPT1 gene and inhibition of Pim-3 signaling pathway in lung cancer cells.

Based on the concept of hypocrisy perception, this paper studies and discusses consumers’ response to corporate social responsibility (CSR) hypocrisy perception, discusses the formation of consumers’ hypocrisy perception from the perspective of consumers’ expectation of CSR, and originally reveals the psychological and behavioral mechanisms of the generation of negative emotions and their role in consumer response. The results are as follows: (1) consumers’ CSR expectations and CSR perceived performance have a significant impact on their perception of hypocrisy; (2) consumers’ perception of hypocrisy has a significant impact on their negative emotions; (3) consumers’ negative emotions can have a significant impact on their attitudes and negative behaviors. The research results show that consumers’ expectations of CSR activities can affect consumers’ attitudes and behaviors, among which consumers’ perceived CSR performance, perceived hypocrisy, and negative emotions play an important role. In the implementation of CSR activities, enterprises should avoid making consumers have excessive expectations and appease their negative emotions, so as to improve the implementation effect of CSR activities.

Companies may be confused by consumers’ perceptions of hypocrisy related to corporate social responsibility activities. This study investigates the mechanism of consumer response to perceived hypocrisy and determines that internal attribution and negative emotion play vital roles. The findings may be summarized as follows. First, the consumers’ internal attribution of corporate hypocrisy increases the perceived hypocrisy. Second, the consumers’ internal attribution of corporate hypocrisy also increases their negative emotions. Third, consumers’ perceptions of hypocrisy intensify their negative emotions. Fourth, the negative emotions of consumers negatively influence their attitude toward companies. Finally, consumers’ negative emotions increase their negative behavior. The findings demonstrate that the influence of perceived hypocrisy on consumers’ attitude and behavior is affected by negative emotion. Consumers’ internal attribution of corporate hypocrisy affects their perceptions of hypocrisy, prompting negative emotions. The psychological and behavioral mechanism of consumer response to perceived hypocrisy is discussed, thus contributing to the study of corporate hypocrisy.

In order to reveal the nonlinear dynamics behavior of the transmission mechanism of the roller movable tooth. A translational-pure torsional coupled dynamics model is established with considering the number of movable tooth,the gap between the teeth and the dynamic loads. The relative displacement between the various parts of the meshing line is derived. The system nonlinear dynamics differential equations and coordinate transformation of differential equations are worked,and the non-dimensional treatment is carried out. The dimensionless differential equations is solved by the numerical analysis software,the phase diagram and Poincare diagram are obtained. The influence of parameter variation on the nonlinear characteristics of the system is analyzed by change the parameter of eccentric distance and meshing damping. The results show that the movement of system is different by the changing of the eccentric distance. With the reducing of the eccentric distance,the system enters the periodic movement through the chaotic motion,and then enters the chaotic motion through the quasi-periodic. If the eccentric distance is not choose bigger and smaller,the vibration response amplitude of the mechanism is reduced,the stability of the system can be improved.

According to the security problem of Web user information, the user behavior was analyzed and authenticated by the method of machine learning. First of all, through the principal component analysis to reduce the dimension of the original data set, then use the SVM algorithm to allow the computer to learn the history of user behavior evidence, to get a model to identify the user's identity. The practical application and theoretical analysis show that the model in user behavior identification authentication, can be more accurate and efficient classification of dangerous users and trusted users, analysis lay a solid theoretical and practical basis for the high performance user behavior such as electronic commerce, network finance and other key of Internet applications.