Search

Your search keyword '"Wang Kenneth K."' showing total 2,236 results
Start Over Author "Wang Kenneth K."
2,236 results on '"Wang Kenneth K."'

1. Photodynamic priming with triple-receptor targeted nanoconjugates that trigger T cell-mediated immune responses in a 3D in vitro heterocellular model of pancreatic cancer

Author

De Silva Pushpamali, Bano Shazia, Pogue Brian W., Wang Kenneth K., Maytin Edward V., and Hasan Tayyaba

Subjects
immunogenic cell death, multitargeting, photodynamic therapy, photoimmuno-nano-conjugates, t cell priming, tumor heterogeneity, Physics, QC1-999
Abstract

Photodynamic priming (PDP), a collateral effect of photodynamic therapy, can transiently alter the tumor microenvironment (TME) beyond the cytotoxic zone. Studies have demonstrated that PDP increases tumor permeability and modulates immune-stimulatory effects by inducing immunogenic cell death, via the release of damage-associated molecular patterns and tumor-associated antigens. Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest of cancers with a stubborn immunosuppressive TME and a dense stroma, representing a challenge for current molecular targeted therapies often involving macromolecules. We, therefore, tested the hypothesis that PDP’s TME modulation will enable targeted therapy and result in immune stimulation. Using triple-receptor-targeted photoimmuno-nanoconjugate (TR-PINs)-mediated PDP, targeting epidermal growth factor receptor, transferrin receptor, and human epidermal growth factor receptor 2 we show light dose-dependent TR-PINs mediated cytotoxicity in human PDAC cells (MIA PaCa-2), co-cultured with human pancreatic cancer-associated fibroblasts (PCAFs) in spheroids. Furthermore, TR-PINs induced the expression of heat shock proteins (Hsp60, Hsp70), Calreticulin, and high mobility group box 1 in a light dose and time-dependent manner. TR-PINs-mediated T cell activation was observed in co-cultures of immune cells with the MIA PaCa-2-PCAF spheroids. Both CD4+ T and CD8+ T cells showed light dose and time-dependant antitumor reactivity by upregulating degranulation marker CD107a and interferon-gamma post-PDP. Substantial tumor cell death in immune cell-spheroid co-cultures by day 3 shows the augmentation by antitumor T cell activation and their ability to recognize tumors for a light dose-dependent kill. These data confirm enhanced destruction of heterogeneous pancreatic spheroids mediated by PDP-induced phototoxicity, TME modulation and increased immunogenicity with targeted nanoconstructs.

Published
2021
Full Text
View/download PDF

2. Genomic signatures of past and present chromosomal instability in Barrett’s esophagus and early esophageal adenocarcinoma

Author

Bao, Chunyang, Tourdot, Richard W, Brunette, Gregory J, Stewart, Chip, Sun, Lili, Baba, Hideo, Watanabe, Masayuki, Agoston, Agoston T, Jajoo, Kunal, Davison, Jon M, Nason, Katie S, Getz, Gad, Wang, Kenneth K, Imamura, Yu, Odze, Robert, Bass, Adam J, Stachler, Matthew D, and Zhang, Cheng-Zhong

Subjects
Biological Sciences, Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Genetics, Human Genome, Rare Diseases, Cancer, Digestive Diseases, 2.1 Biological and endogenous factors, Aetiology, Humans, Barrett Esophagus, Esophageal Neoplasms, Adenocarcinoma, Chromosomal Instability, Precancerous Conditions, Genomics, Disease Progression
Abstract

The progression of precancerous lesions to malignancy is often accompanied by increasing complexity of chromosomal alterations but how these alterations arise is poorly understood. Here we perform haplotype-specific analysis of chromosomal copy-number evolution in the progression of Barrett's esophagus (BE) to esophageal adenocarcinoma (EAC) on multiregional whole-genome sequencing data of BE with dysplasia and microscopic EAC foci. We identify distinct patterns of copy-number evolution indicating multigenerational chromosomal instability that is initiated by cell division errors but propagated only after p53 loss. While abnormal mitosis, including whole-genome duplication, underlies chromosomal copy-number changes, segmental alterations display signatures of successive breakage-fusion-bridge cycles and chromothripsis of unstable dicentric chromosomes. Our analysis elucidates how multigenerational chromosomal instability generates copy-number variation in BE cells, precipitates complex alterations including DNA amplifications, and promotes their independent clonal expansion and transformation. In particular, we suggest sloping copy-number variation as a signature of ongoing chromosomal instability that precedes copy-number complexity. These findings suggest copy-number heterogeneity in advanced cancers originates from chromosomal instability in precancerous cells and such instability may be identified from the presence of sloping copy-number variation in bulk sequencing data.

Published
2023

7. Prospective Study Assessing Impact of Ethylene Oxide Sterilization on Endoscopic Ultrasound Image Quality

Author

Majumder, Shounak, Long, Zaiyang, Hooke, Alexander W., Petersen, Bret T., Gleeson, Ferga C., Bruno, Marco, DeWitt, John, Elta, Grace, Fuji, Larissa, Gomez, Victoria, Palazzo, Laurent, Shami, Vanessa M., Stevens, Tyler, Topazian, Mark D., Wiersema, Maurits J., Berglund, Lawrence J., Abu Dayyeh, Barham K., Chandrasekhara, Vinay, Iyer, Prasad G., Rajan, Elizabeth, Storm, Andrew C., Wang, Kenneth K., Lennon, Ryan, Larson, Joseph J., Enders, Felicity T., Frein, Jed R., Yates, Raymond A., Hangiandreou, Nicholas J., and Levy, Michael J.

Published
2022
Full Text
View/download PDF

8. Optimized Surveillance Intervals Following Endoscopic Eradication of Dysplastic Barrett’s Esophagus: An International Cohort Study

Author

Kahn, Allon, Crook, Julia, Heckman, Michael G., Wieczorek, Mikolaj A., Sami, Sarmed, Snyder, Diana, Agarwal, Siddharth, Santiago, Jose, Fernandez-Sordo, Jacobo Ortiz, Tan, W. Keith, Lansing, Ramona, Wang, Kenneth K., Ragunath, Krish, DiPietro, Massimiliano, Wolfsen, Herbert, Ramirez, Francisco, Fleischer, David, Leggett, Cadman L., and Iyer, Prasad G.

Published
2022
Full Text
View/download PDF

12. Feasibility and Safety of Tethered Capsule Endomicroscopy in Patients With Barrett’s Esophagus in a Multi-Center Study

Author

Dong, Jing, Grant, Catriona, Vuong, Barry, Nishioka, Norman, Gao, Anna Huizi, Beatty, Matthew, Baldwin, Grace, Baillargeon, Aaron, Bablouzian, Ara, Grahmann, Patricia, Bhat, Nitasha, Ryan, Emily, Barrios, Amilcar, Giddings, Sarah, Ford, Timothy, Beaulieu-Ouellet, Emilie, Hosseiny, Seyed Hamid, Lerman, Irene, Trasischker, Wolfgang, Reddy, Rohith, Singh, Kanwarpal, Gora, Michalina, Hyun, Daryl, Quénéhervé, Lucille, Wallace, Michael, Wolfsen, Herbert, Sharma, Prateek, Wang, Kenneth K., Leggett, Cadman L., Poneros, John, Abrams, Julian A., Lightdale, Charles, Leeds, Samantha, Rosenberg, Mireille, and Tearney, Guillermo J.

Published
2022
Full Text
View/download PDF

14. Comparative Molecular Analysis of Gastrointestinal Adenocarcinomas

Author

Liu, Yang, Sethi, Nilay S, Hinoue, Toshinori, Schneider, Barbara G, Cherniack, Andrew D, Sanchez-Vega, Francisco, Seoane, Jose A, Farshidfar, Farshad, Bowlby, Reanne, Islam, Mirazul, Kim, Jaegil, Chatila, Walid, Akbani, Rehan, Kanchi, Rupa S, Rabkin, Charles S, Willis, Joseph E, Wang, Kenneth K, McCall, Shannon J, Mishra, Lopa, Ojesina, Akinyemi I, Bullman, Susan, Pedamallu, Chandra Sekhar, Lazar, Alexander J, Sakai, Ryo, Network, The Cancer Genome Atlas Research, Caesar-Johnson, Samantha J, Demchok, John A, Felau, Ina, Kasapi, Melpomeni, Ferguson, Martin L, Hutter, Carolyn M, Sofia, Heidi J, Tarnuzzer, Roy, Wang, Zhining, Yang, Liming, Zenklusen, Jean C, Zhang, Jiashan, Chudamani, Sudha, Liu, Jia, Lolla, Laxmi, Naresh, Rashi, Pihl, Todd, Sun, Qiang, Wan, Yunhu, Wu, Ye, Cho, Juok, DeFreitas, Timothy, Frazer, Scott, Gehlenborg, Nils, Getz, Gad, Heiman, David I, Lawrence, Michael S, Lin, Pei, Meier, Sam, Noble, Michael S, Saksena, Gordon, Voet, Doug, Zhang, Hailei, Bernard, Brady, Chambwe, Nyasha, Dhankani, Varsha, Knijnenburg, Theo, Kramer, Roger, Leinonen, Kalle, Liu, Yuexin, Miller, Michael, Reynolds, Sheila, Shmulevich, Ilya, Thorsson, Vesteinn, Zhang, Wei, Broom, Bradley M, Hegde, Apurva M, Ju, Zhenlin, Korkut, Anil, Li, Jun, Liang, Han, Ling, Shiyun, Liu, Wenbin, Lu, Yiling, Mills, Gordon B, Ng, Kwok-Shing, Rao, Arvind, Ryan, Michael, Wang, Jing, Weinstein, John N, Zhang, Jiexin, Abeshouse, Adam, Armenia, Joshua, Chakravarty, Debyani, Chatila, Walid K, Bruijn, Inode, Gao, Jianjiong, Gross, Benjamin E, Heins, Zachary J, Kundra, Ritika, La, Konnor, and Ladanyi, Marc

Subjects
Biological Sciences, Genetics, Digestive Diseases, Rare Diseases, Colo-Rectal Cancer, Cancer Genomics, Human Genome, Cancer, Genetic Testing, Biotechnology, Adenocarcinoma, Aneuploidy, Chromosomal Instability, DNA Methylation, DNA Polymerase II, DNA-Binding Proteins, Epigenesis, Genetic, Female, Gastrointestinal Neoplasms, Gene Regulatory Networks, Heterogeneous-Nuclear Ribonucleoproteins, Humans, Male, Microsatellite Instability, MutL Protein Homolog 1, Mutation, Poly-ADP-Ribose Binding Proteins, Polymorphism, Single Nucleotide, Proto-Oncogene Proteins p21(ras), RNA-Binding Proteins, SOX9 Transcription Factor, Cancer Genome Atlas Research Network, cancer, colon, colorectal, epigenetic, esophagus, genomic, methylation, rectum, stomach, tumor, Neurosciences, Oncology and Carcinogenesis, Oncology & Carcinogenesis, Biochemistry and cell biology, Oncology and carcinogenesis
Abstract

We analyzed 921 adenocarcinomas of the esophagus, stomach, colon, and rectum to examine shared and distinguishing molecular characteristics of gastrointestinal tract adenocarcinomas (GIACs). Hypermutated tumors were distinct regardless of cancer type and comprised those enriched for insertions/deletions, representing microsatellite instability cases with epigenetic silencing of MLH1 in the context of CpG island methylator phenotype, plus tumors with elevated single-nucleotide variants associated with mutations in POLE. Tumors with chromosomal instability were diverse, with gastroesophageal adenocarcinomas harboring fragmented genomes associated with genomic doubling and distinct mutational signatures. We identified a group of tumors in the colon and rectum lacking hypermutation and aneuploidy termed genome stable and enriched in DNA hypermethylation and mutations in KRAS, SOX9, and PCBP1.

Published
2018

24. Application of artificial intelligence using a novel EUS-based convolutional neural network model to identify and distinguish benign and malignant hepatic masses

Author

Marya, Neil B., Powers, Patrick D., Fujii-Lau, Larissa, Abu Dayyeh, Barham K., Gleeson, Ferga C., Chen, Shigao, Long, Zaiyang, Hough, David M., Chandrasekhara, Vinay, Iyer, Prasad G., Rajan, Elizabeth, Sanchez, William, Sawas, Tarek, Storm, Andrew C., Wang, Kenneth K., and Levy, Michael J.

Published
2021
Full Text
View/download PDF

25. Late Recurrence of Barrett’s Esophagus After Complete Eradication of Intestinal Metaplasia is Rare: Final Report From Ablation in Intestinal Metaplasia Containing Dysplasia Trial

Author

Cotton, Cary C, Wolf, W Asher, Overholt, Bergein F, Li, Nan, Lightdale, Charles J, Wolfsen, Herbert C, Pasricha, Sarina, Wang, Kenneth K, Shaheen, Nicholas J, Group, AIM Dysplasia Trial, Sampliner, Richard E, Fleischer, David E, Sharma, Virender K, Eisen, Glenn M, Fennerty, M Brian, Hunter, John G, Bronner, Mary P, Goldblum, John R, Bennett, Ana E, Mashimo, Hiroshi, Rothstein, Richard I, Gordon, Stuart R, Edmundowicz, Steven A, Muthusamy, V Raman, Chang, Kenneth J, Kimmey, Michael B, Spechler, Stuart J, Siddiqui, Ali A, Souza, Rhonda F, Infantolino, Anthony, Dumot, John A, Falk, Gary W, Jobe, Blair A, Hawes, Robert H, Hoffman, Brenda J, Sharma, Prateek, and Chak, Amitabh

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Prevention, Clinical Trials and Supportive Activities, Clinical Research, Digestive Diseases, Cancer, Oral and gastrointestinal, Aged, Barrett Esophagus, Catheter Ablation, Disease Progression, Esophagus, Female, Follow-Up Studies, Humans, Incidence, Kaplan-Meier Estimate, Male, Metaplasia, Middle Aged, Mucous Membrane, Population Surveillance, Prospective Studies, Recurrence, Time Factors, AIM Dysplasia Trial Group, HGD, High Grade Dysplasia, LGD, Long-term Outcome, Low Grade Dysplasia, Prognostic Factor, Neurosciences, Paediatrics and Reproductive Medicine, Gastroenterology & Hepatology, Clinical sciences, Nutrition and dietetics
Abstract

Background & aimsThe goal of treatment for Barrett's esophagus (BE) with dysplasia is complete eradication of intestinal metaplasia (CEIM). The long-term durability of CEIM has not been well characterized, so the frequency and duration of surveillance are unclear. We report results from a 5-year follow-up analysis of patients with BE and dysplasia treated by radiofrequency ablation (RFA) in the randomized controlled Ablation of Intestinal Metaplasia Containing Dysplasia (AIM) trial.MethodsParticipants for the AIM Dysplasia trial (18-80 years old) were recruited from 19 sites in the United States and had endoscopic evidence of non-nodular dysplastic BE ≤8 cm in length. Subjects (n = 127) were randomly assigned (2:1 ratio) to receive either RFA (entire BE segment ablated circumferentially) or a sham endoscopic procedure; patients in the sham group were offered RFA treatment 1 year later, and all patients were followed for 5 years. We collected data on BE recurrence (defined as intestinal metaplasia in the tubular esophagus) and dysplastic BE recurrence among patients who achieved CEIM. We constructed Kaplan-Meier estimates and applied parametric survival analysis to examine proportions of patients without any recurrence and without dysplastic recurrence.ResultsOf 127 patients in the AIM Dysplasia trial, 119 received RFA and met inclusion criteria. Of those 119, 110 (92%) achieved CEIM. Over 401 person-years of follow-up (mean, 3.6 years per patient; range, 0.2-5.8 years), 35 of 110 (32%) patients had recurrence of BE or dysplasia, and 19 (17%) had dysplasia recurrence. The incidence rate of BE recurrence was 10.8 per 100 person-years overall (95% CI, 7.8-15.0); 8.3 per 100 person-years among patients with baseline low-grade dysplasia (95% CI, 4.9-14.0), and 13.5 per 100 person-years among patients with baseline high-grade dysplasia (95% CI 8.8-20.7). The incidence rate of dysplasia recurrence was 5.2 per 100 person-years overall (95% CI 3.3-8.2); 3.3 per 100 person-years among patients with baseline low-grade dysplasia (95% CI 1.5-7.2), and 7.3 per 100 person-years among patients with baseline high-grade dysplasia (95% CI 4.2-12.5). Neither BE nor dysplasia recurred at a constant rate. There was a greater probability of recurrence in the first year following CEIM than in the following 4 years combined.ConclusionsIn this analysis of prospective cohort data from the AIM Dysplasia trial, we found BE to recur after CEIM by RFA in almost one third of patients with baseline dysplastic disease; most recurrences occurred during the first year after CEIM. However, patients who achieved CEIM and remained BE free at 1 year after RFA had a low risk of BE recurrence. Studies are needed to determine when surveillance can be decreased or discontinued; our study did not identify any BE or dysplasia recurrence after 4 years of surveillance.

Published
2017

27. Outcomes of radiofrequency ablation by manual versus self-sizing circumferential balloon catheters for the treatment of dysplastic Barrett’s esophagus: a multicenter comparative cohort study

Author

Kahn, Allon, Priyan, Harshith, Dierkhising, Ross A., Johnson, Michele L., Lansing, Ramona M., Maixner, Kristyn A., Wolfsen, Herbert C., Wallace, Michael B., Ramirez, Francisco C., Fleischer, David E., Leggett, Cadman L., Wang, Kenneth K., and Iyer, Prasad G.

Published
2021
Full Text
View/download PDF

28. Rates of Recurrent Intestinal Metaplasia and Dysplasia After Successful Endoscopic Therapy of Barrett's Neoplasia by Endoscopic Mucosal Resection vs Endoscopic Submucosal Dissection and Ablation: A Large North American Multicenter Cohort.

Author

Kornpong Vantanasiri, Joseph, Abel, Sachdeva, Karan, Goyal, Rohit, Garg, Nikita, Adoor, Dayyan, Kamboj, Amrit K., Codipilly, D. Chamil, Leggett, Cadman, Wang, Kenneth K., Harmsen, William, Hayat, Umar, Chak, Amitabh, Bhatt, Amit, and Iyer, Prasad G.

Published
2024
Full Text
View/download PDF

31. Risk factors for serious adverse events associated with multiband mucosectomy in Barrett’s esophagus: an international multicenter analysis of 3827 endoscopic resection procedures

Author

Belghazi, Kamar, Marcon, Norman, Teshima, Christopher, Wang, Kenneth K., Milano, Reza V., Mostafavi, Nahid, Wallace, Michael B., Kandel, Pujan, Mejía Pérez, Lady Katherine, Bourke, Michael J., Bahin, Farzan, Everson, Martin A., Haidry, Rehan, Ginsberg, Gregory G., Ma, Gene K., Koch, Arjun D., Ragunath, Krish, Ortiz-Fernandez-Sordo, Jacobo, di Pietro, Massimiliano, Seewald, Stefan, Weusten, Bas L., Schoon, Erik J., Bisschops, Raf, Bergman, Jacques J., and Pouw, Roos E.

Published
2020
Full Text
View/download PDF

32. Notch Signaling Mediates Differentiation in Barrett’s Esophagus and Promotes Progression to Adenocarcinoma

Author

Kunze, Bettina, Wein, Frederik, Fang, Hsin-Yu, Anand, Akanksha, Baumeister, Theresa, Strangmann, Julia, Gerland, Sophie, Ingermann, Jonas, Münch, Natasha Stephens, Wiethaler, Maria, Sahm, Vincenz, Hidalgo-Sastre, Ana, Lange, Sebastian, Lightdale, Charles J., Bokhari, Aqiba, Falk, Gary W., Friedman, Richard A., Ginsberg, Gregory G., Iyer, Prasad G., Jin, Zhezhen, Nakagawa, Hiroshi, Shawber, Carrie J., Nguyen, TheAnh, Raab, William J., Dalerba, Piero, Rustgi, Anil K., Sepulveda, Antonia R., Wang, Kenneth K., Schmid, Roland M., Wang, Timothy C., Abrams, Julian A., and Quante, Michael

Published
2020
Full Text
View/download PDF

33. Outcomes of patients with submucosal (T1b) esophageal adenocarcinoma: a multicenter cohort study

Author

Otaki, Fouad, Ma, Gene K., Krigel, Anna, Dierkhising, Ross A., Lewis, Jason T., Blevins, Christopher H., Gopalakrishnan, Naveen P., Ravindran, Adharsh, Johnson, Michele L., Leggett, Cadman L., Wigle, Denis, Wang, Kenneth K., Falk, Gary W., Abrams, Julian A., Nakagawa, Hiroshi, Rustgi, Anil K., Wang, Timothy C., Lightdale, Charles J., Ginsberg, Gregory G., and Iyer, Prasad G.

Published
2020
Full Text
View/download PDF

36. Clinical impact of celiac ganglia metastasis upon pancreatic ductal adenocarcinoma

Author

Malikowski, Thomas, Lehrke, Heidi D., Henry, Michael R., Gleeson, Ferga C., Alberts, Steven R., Kendrick, Michael L., Lennon, Ryan J., McWilliams, Robert R., Takahashi, Naoki, Topazian, Mark D., Gara, Naveen, Abu Dayyeh, Barham K., Chandrasekhara, Vinay, Chari, Suresh T., Iyer, Prasad G., Rajan, Elizabeth, Storm, Andrew C., Wang, Kenneth K., and Levy, Michael J.

Published
2020
Full Text
View/download PDF

38. Ligand-Induced Proton Transfer and Low-Barrier Hydrogen Bond Revealed by X‑ray Crystallography

Author

Nichols, Derek A, Hargis, Jacqueline C, Sanishvili, Ruslan, Jaishankar, Priyadarshini, Defrees, Kyle, Smith, Emmanuel W, Wang, Kenneth K, Prati, Fabio, Renslo, Adam R, Woodcock, H Lee, and Chen, Yu

Subjects
Crystallography, X-Ray, Escherichia coli, Hydrogen Bonding, Models, Molecular, Protein Conformation, Protons, beta-Lactamases, Chemical Sciences, General Chemistry
Abstract

Ligand binding can change the pKa of protein residues and influence enzyme catalysis. Herein, we report three ultrahigh resolution X-ray crystal structures of CTX-M β-lactamase, directly visualizing protonation state changes along the enzymatic pathway: apo protein at 0.79 Å, precovalent complex with nonelectrophilic ligand at 0.89 Å, and acylation transition state (TS) analogue at 0.84 Å. Binding of the noncovalent ligand induces a proton transfer from the catalytic Ser70 to the negatively charged Glu166, and the formation of a low-barrier hydrogen bond (LBHB) between Ser70 and Lys73, with a length of 2.53 Å and the shared hydrogen equidistant from the heteroatoms. QM/MM reaction path calculations determined the proton transfer barrier to be 1.53 kcal/mol. The LBHB is absent in the other two structures although Glu166 remains neutral in the covalent complex. Our data represents the first X-ray crystallographic example of a hydrogen engaged in an enzymatic LBHB, and demonstrates that desolvation of the active site by ligand binding can provide a protein microenvironment conducive to LBHB formation. It also suggests that LBHBs may contribute to stabilization of the TS in general acid/base catalysis together with other preorganized features of enzyme active sites. These structures reconcile previous experimental results suggesting alternatively Glu166 or Lys73 as the general base for acylation, and underline the importance of considering residue protonation state change when modeling protein-ligand interactions. Additionally, the observation of another LBHB (2.47 Å) between two conserved residues, Asp233 and Asp246, suggests that LBHBs may potentially play a special structural role in proteins.

Published
2015

39. Use of probe‐based confocal laser endomicroscopy (pCLE) in gastrointestinal applications. A consensus report based on clinical evidence

Author

Wang, Kenneth K, Carr-Locke, David L, Singh, Satish K, Neumann, Helmut, Bertani, Helga, Galmiche, Jean-Paul, Arsenescu, Razvan I, Caillol, Fabrice, Chang, Kenneth J, Chaussade, Stanislas, Coron, Emmanuel, Costamagna, Guido, Dlugosz, Aldona, Gan, S Ian, Giovannini, Marc, Gress, Frank G, Haluszka, Oleh, Ho, Khek Y, Kahaleh, Michel, Konda, Vani J, Prat, Frederic, Shah, Raj J, Sharma, Prateek, Slivka, Adam, Wolfsen, Herbert C, and Zfass, Alvin

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Clinical Research, Digestive Diseases, Endomicroscopy, gastroenterology, recommendations, Barrett's oesophagus, biliary strictures, colorectal polyps, small polyps, inflammatory bowel diseases, training, credentialling, advanced endoscopy, optical biopsy, Barrett’s oesophagus, Clinical sciences
Abstract

BackgroundProbe-based confocal laser endomicroscopy (pCLE) provides microscopic imaging during an endoscopic procedure. Its introduction as a standard modality in gastroenterology has brought significant progress in management strategies, affecting many aspects of clinical care and requiring standardisation of practice and training.ObjectiveThis study aimed to provide guidance on the standardisation of its practice and training in Barrett's oesophagus, biliary strictures, colorectal lesions and inflammatory bowel diseases.MethodsInitial statements were developed by five group leaders, based on the available clinical evidence. These statements were then voted and edited by the 26 participants, using a modified Delphi approach. After two rounds of votes, statements were validated if the threshold of agreement was higher than 75%.ResultsTwenty-six experts participated and, among a total of 77 statements, 61 were adopted (79%) and 16 were rejected (21%). The adoption of each statement was justified by the grade of evidence.ConclusionpCLE should be used to enhance the diagnostic arsenal in the evaluation of these indications, by providing microscopic information which improves the diagnostic performance of the physician. In order actually to implement this technology in the clinical routine, and to ensure good practice, standardised initial and continuing institutional training programmes should be established.

Published
2015

40. EUS-derived criteria for distinguishing benign from malignant metastatic solid hepatic masses.

Author

Fujii-Lau, Larissa L, Abu Dayyeh, Barham K, Bruno, Marco J, Chang, Kenneth J, DeWitt, John M, Fockens, Paul, Forcione, David, Napoleon, Bertrand, Palazzo, Laurent, Topazian, Mark D, Wiersema, Maurits J, Chak, Amitabh, Clain, Jonathan E, Faigel, Douglas O, Gleeson, Ferga C, Hawes, Robert, Iyer, Prasad G, Rajan, Elizabeth, Stevens, Tyler, Wallace, Michael B, Wang, Kenneth K, and Levy, Michael J

Subjects
Liver, Humans, Liver Neoplasms, Endosonography, Neoplasm Staging, Retrospective Studies, Aged, Middle Aged, Female, Male, Endoscopic Ultrasound-Guided Fine Needle Aspiration, Clinical Sciences, Gastroenterology & Hepatology
Abstract

BackgroundDetection of hepatic metastases during EUS is an important component of tumor staging.ObjectiveTo describe our experience with EUS-guided FNA (EUS-FNA) of solid hepatic masses and derive and validate criteria to help distinguish between benign and malignant hepatic masses.DesignRetrospective study, survey.SettingSingle, tertiary-care referral center.PatientsMedical records were reviewed for all patients undergoing EUS-FNA of solid hepatic masses over a 12-year period.InterventionsEUS-FNA of solid hepatic masses.Main outcome measurementsMasses were deemed benign or malignant according to predetermined criteria. EUS images from 200 patients were used to create derivation and validation cohorts of 100 cases each, matched by cytopathologic diagnosis. Ten expert endosonographers blindly rated 15 initial endosonographic features of each of the 100 images in the derivation cohort. These data were used to derive an EUS scoring system that was then validated by using the validation cohort by the expert endosonographer with the highest diagnostic accuracy.ResultsA total of 332 patients underwent EUS-FNA of a hepatic mass. Interobserver agreement regarding the initial endosonographic features among the expert endosonographers was fair to moderate, with a mean diagnostic accuracy of 73% (standard deviation 5.6). A scoring system incorporating 7 EUS features was developed to distinguish benign from malignant hepatic masses by using the derivation cohort with an area under the receiver operating curve (AUC) of 0.92; when applied to the validation cohort, performance was similar (AUC 0.86). The combined positive predictive value of both cohorts was 88%.LimitationsSingle center, retrospective, only one expert endosonographer deriving and validating the EUS criteria.ConclusionAn EUS scoring system was developed that helps distinguish benign from malignant hepatic masses. Further study is required to determine the impact of these EUS criteria among endosonographers of all experience.

Published
2015

41. Safety, Diagnostic Accuracy, and Effects of Endoscopic Ultrasound Fine-Needle Aspiration on Detection of Extravascular Migratory Metastases

Author

Rustagi, Tarun, Gleeson, Ferga C., Chari, Suresh T., Lehrke, Heidi D., Takahashi, Naoki, Malikowski, Thomas M., Abu Dayyeh, Barham K., Chandrasekhara, Vinay, Iyer, Prasad G., Kendrick, Michael L., Pearson, Randall K., Petersen, Bret T., Rajan, Elizabeth, Smoot, Rory L., Storm, Andrew C., Topazian, Mark D., Truty, Mark J., Vege, Santhi S., Wang, Kenneth K., and Levy, Michael J.

Published
2019
Full Text
View/download PDF

46. Combined Celiac Ganglia and Plexus Neurolysis Shortens Survival, Without Benefit, vs Plexus Neurolysis Alone

Author

Levy, Michael J., Gleeson, Ferga C., Topazian, Mark D., Fujii-Lau, Larissa L., Enders, Felicity T., Larson, Joseph J., Mara, Kristin, Abu Dayyeh, Barham K., Alberts, Steven R., Hallemeier, Christopher L., Iyer, Prasad G., Kendrick, Michael L., Mauck, William D., Pearson, Randall K., Petersen, Bret T., Rajan, Elizabeth, Takahashi, Naoki, Vege, Santhi S., Wang, Kenneth K., and Chari, Suresh T.

Published
2019
Full Text
View/download PDF

48. Identification of Prognostic Phenotypes of Esophageal Adenocarcinoma in 2 Independent Cohorts

Author

Noorani, Ayesha, Edwards, Paul A.W., Grehan, Nicola, Nutzinger, Barbara, Hughes, Caitriona, Fidziukiewicz, Elwira, Bornschein, Jan, MacRae, Shona, Crawte, Jason, Contino, Gianmarco, Li, Xiaodun, Rue, Rachel de la, O’Donovan, Maria, Miremad, Ahmad, Malhotra, Shalini, Tripathi, Monika, Tavaré, Simon, Lynch, Andy G., Eldridge, Matthew, Secrier, Maria, Bower, Lawrence, Devonshire, Ginny, Perner, Juliane, Jammula, Sriganesh, Davies, Jim, Crichton, Charles, Carroll, Nick, Safranek, Peter, Hindmarsh, Andrew, Sujendran, Vijayendran, Hayes, Stephen J., Ang, Yeng, Preston, Shaun R., Oakes, Sarah, Bagwan, Izhar, Save, Vicki, Skipworth, Richard J.E., Hupp, Ted R., O’Neill, J. Robert, Tucker, Olga, Beggs, Andrew, Taniere, Philippe, Puig, Sonia, Underwood, Timothy J., Noble, Fergus, Owsley, Jack, Barr, Hugh, Shepherd, Neil, Old, Oliver, Lagergren, Jesper, Gossage, James, Davies, Andrew, Chang, Fuju, Zylstra, Janine, Mahadeva, Ula, Goh, Vicky, Ciccarelli, Francesca D., Sanders, Grant, Berrisford, Richard, Harden, Catherine, Bunting, David, Lewis, Mike, Cheong, Ed, Kumar, Bhaskar, Parsons, Simon L., Soomro, Irshad, Kaye, Philip, Saunders, John, Lovat, Laurence, Haidry, Rehan, Eneh, Victor, Igali, Laszlo, Scott, Michael, Sothi, Sharmila, Suortamo, Sari, Lishman, Suzy, Hanna, George B., Peters, Christopher J., Grabowska, Anna, Turkington, Richard, Sawas, Tarek, Killcoyne, Sarah, Iyer, Prasad G., Wang, Kenneth K., Smyrk, Thomas C., Kisiel, John B., Qin, Yi, Ahlquist, David A., Rustgi, Anil K., Costa, Rui J., Gerstung, Moritz, Fitzgerald, Rebecca C., and Katzka, David A.

Published
2018
Full Text
View/download PDF

49. A prospective multicenter study using a new multiband mucosectomy device for endoscopic resection of early neoplasia in Barrett’s esophagus

Author

Pouw, Roos E., Beyna, Torsten, Belghazi, Kamar, Koch, Arjun D., Schoon, Erik J., Haidry, Rehan, Weusten, Bas L., Bisschops, Raf, Shaheen, Nicholas J., Wallace, Michael B., Marcon, Norman, Heise-Ginsburg, Rachel, Gotink, Anniek W., Wang, Kenneth K., Leggett, Cadman L., Ortiz-Fernández-Sordo, Jacobo, Ragunath, Krish, DiPietro, Massimiliano, Pech, Oliver, Neuhaus, Horst, and Bergman, Jacques J.

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results