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22 results on '"Wang, Zuozhen"'

22. [Screening for mutants with thermostabe lipase A from Burkholderia sp. ZYB002].

Author

Liu Y, Qiu L, Huang J, Zhao B, Wang Z, Zhu X, Gao Y, and Shu Z

Subjects
Amino Acid Sequence, Bacterial Proteins metabolism, Burkholderia chemistry, Burkholderia genetics, Enzyme Stability, Hot Temperature, Kinetics, Lipase metabolism, Molecular Sequence Data, Mutagenesis, Mutation, Protein Engineering, Sequence Alignment, Bacterial Proteins chemistry, Bacterial Proteins genetics, Burkholderia enzymology, Lipase chemistry, Lipase genetics
Abstract

Objective: We improved the thermostability of LipA from Burkholderia cecapia ZYB002 by protein engineering technology to expand the application of lipase LipA., Method: On the basis of B-factor value of lipase LipA, series of potential mutation hotspots were selected for iterative saturation mutagenesis and the corresponding small mutation gene libraries were then constructed to screen the hyperthermal variants., Results: From the above mutation libraries, we obtained a series of mutants whose enzyme half-life at 55 degrees C increased by 1.7 to 2.2-fold., Conclusion: B-factor iterative test (B-FIT) is feasible to mutate thermostable strains.

Published
2015

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