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712 results on '"Wang, Zhenling"'

2. SARS-CoV-2 Delta and Omicron variants resist spike cleavage by human airway trypsin-like protease

Author

Ren, Wenyan, Hong, Weiqi, Yang, Jingyun, Zou, Jun, Chen, Li, Zhou, Yanan, Lei, Hong, Alu, Aqu, Que, Haiying, Gong, Yanqiu, Bi, Zhenfei, He, Cai, Fu, Minyang, Peng, Dandan, Yang, Yun, Yu, Wenhai, Tang, Cong, Huang, Qing, Yang, Mengli, Li, Bai, Li, Jingmei, Wang, Junbin, Ma, Xuelei, Hu, Hongbo, Cheng, Wei, Dong, Haohao, Lei, Jian, Chen, Lu, Zhou, Xikun, Li, Jiong, Wang, Wei, Lu, Guangwen, Shen, Guobo, Yang, Li, Yang, Jinliang, Wang, Zhenling, Jia, Guowen, Su, Zhaoming, Shao, Bin, Miao, Hanpei, Lau, Johnson Yiu-Nam, Wei, Yuquan, Zhang, Kang, Dai, Lunzhi, Lu, Shuaiyao, and Wei, Xiawei

Subjects
Medical research, Medicine, Experimental, Proteases -- Health aspects, Viral proteins -- Physiological aspects, Airway (Medicine) -- Health aspects
Abstract

Soluble host factors in the upper respiratory tract can serve as the first line of defense against SARS-CoV-2 infection. In this study, we described the identification and function of a human airway trypsin-like protease (HAT), capable of reducing the infectivity of ancestral SARS-CoV-2. Further, in mouse models, HAT analogue expression was upregulated by SARS- CoV-2 infection. The antiviral activity of HAT functioned through the cleavage of the SARS-CoV-2 spike glycoprotein at R682. This cleavage resulted in inhibition of the attachment of ancestral spike proteins to host cells, which inhibited the cell-cell membrane fusion process. Importantly, exogenous addition of HAT notably reduced the infectivity of ancestral SARS- CoV-2 in vivo. However, HAT was ineffective against the Delta variant and most circulating Omicron variants, including the BQ.1.1 and XBB.1.5 subvariants. We demonstrate that the P681R mutation in Delta and P681H mutation in the Omicron variants, adjacent to the R682 cleavage site, contributed to HAT resistance. Our study reports what we believe to be a novel soluble defense factor against SARS-CoV-2 and resistance of its actions in the Delta and Omicron variants., Introduction The causative agent for COVID-19, SARS-CoV-2, is a single-stranded, positive-sense RNA virus that replicates through the viral RNA-dependent RNA polymerase (RdRp) and the exoribonuclease (ExoN). The combination of RdRp [...]

Published
2024
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3. A linear surrogate for optimising functions of an orthogonal matrix with applications in wave function theory

Author

Wang, Zhenling and Head-Gordon, Martin

Subjects
Chemical Sciences, Physical Chemistry, Surrogate optimisation, orthogonal matrix, orbital localisation, generalised valence bond theory, Atomic, Molecular, Nuclear, Particle and Plasma Physics, Physical Chemistry (incl. Structural), Theoretical and Computational Chemistry, Chemical Physics, Physical chemistry, Theoretical and computational chemistry
Abstract

The technique of surrogate optimisation is to use a simpler function to approximate a complex function that is time-consuming to evaluate. We show that the maximum of a special type of surrogate function (Formula presented.) is at (Formula presented.), and that there is one and only one local maximum both in (Formula presented.) and (Formula presented.). This function (Formula presented.) has been found to be useful in various aspects of electronic structure theory, including proving the Carlson-Keller theorem, and localising orbitals. As one other example, we apply it here to optimise the ground state of molecules using the Generalised Valence Bond wavefunction.

Published
2023

4. Sparsity of the electron repulsion integral tensor using different localized virtual orbital representations in local second-order Møller–Plesset theory

Author

Wang, Zhenling, Aldossary, Abdulrahman, and Head-Gordon, Martin

Subjects
Physical Sciences, Chemical Sciences, Atomic, Molecular and Optical Physics, Physical Chemistry, Engineering, Chemical Physics, Chemical sciences, Physical sciences
Abstract

Utilizing localized orbitals, local correlation theory can reduce the unphysically high system-size scaling of post-Hartree-Fock (post-HF) methods to linear scaling in insulating molecules. The sparsity of the four-index electron repulsion integral (ERI) tensor is central to achieving this reduction. For second-order Møller-Plesset theory (MP2), one of the simplest post-HF methods, only the (ia|jb) ERIs are needed, coupling occupied orbitals i, j and virtuals a, b. In this paper, we compare the numerical sparsity (called the "ragged list") and two other approaches revealing the low-rank sparsity of the ERI. The ragged list requires only one set of (localized) virtual orbitals, and we find that the orthogonal valence virtual-hard virtual set of virtuals originally proposed by Subotnik et al. gives the sparsest ERI tensor. To further compress the ERI tensor, the pair natural orbital (PNO) type representation uses different sets of virtual orbitals for different occupied orbital pairs, while the occupied-specific virtual (OSV) approach uses different virtuals for each occupied orbital. Our results indicate that while the low-rank PNO representation achieves significant rank reduction, it also requires more memory than the ragged list. The OSV approach requires similar memory to that of the ragged list, but it involves greater algorithmic complexity. An approximation (called the "fixed sparsity pattern") for solving the local MP2 equations using the numerically sparse ERI tensor is proposed and tested to be sufficiently accurate and to have highly controllable error. A low-scaling local MP2 algorithm based on the ragged list and the fixed sparsity pattern is therefore promising.

Published
2023

5. Causes of death and treatment-related mortality in newly diagnosed childhood acute lymphoblastic leukemia treatment with Chinese Children’s Cancer Group study ALL-2015

Author

Liu, Kangkang, Shao, Jingbo, Cai, Jiaoyang, Tang, Jingyan, Shen, Shuhong, Xu, Fengling, Ren, Yuanyuan, Zhang, Aijun, Tian, Xin, Lu, Xiaoqian, Hu, Shaoyan, Hu, Qun, Jiang, Hua, Zhou, Fen, Liang, Changda, Leung, Alex Wing Kwan, Zhai, Xiaowen, Li, Chunfu, Fang, Yongjun, Wang, Zhenling, Wen, Lu, Yang, Hui, Wang, Ningling, and Jiang, Hui

Published
2023
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6. Generalization of ETS-NOCV and ALMO-COVP Energy Decomposition Analysis to Connect Any Two Electronic States and Comparative Assessment

Author

Shen, Hengyuan, Wang, Zhenling, and Head-Gordon, Martin

Subjects
Affordable and Clean Energy, Theoretical and Computational Chemistry, Biochemistry and Cell Biology, Computer Software, Chemical Physics
Abstract

Energy decomposition analysis (EDA) is a useful tool for obtaining chemically meaningful insights into molecular interactions. The extended transition-state method with natural orbitals for chemical valence (ETS-NOCV) and the absolutely localized molecular orbital-based method with complementary occupied-virtual pairs (ALMO-COVP) are two successful EDA schemes. Working within ground-state generalized Kohn-Sham density functional theory (DFT), we extend these methods to perform EDA between any two electronic states that can be connected by a unitary transformation of density matrices. A direct proof that the NOCV eigenvalues are symmetric pairs is given, and we also prove that the charge and energy difference defined by ALMO are invariant under certain orbital rotations, allowing us to define COVPs. We point out that ETS is actually a 1-point quadrature to obtain the effective Fock matrix, and though it is reasonably accurate, it can be systematically further improved by adding more quadrature points. We explain why the calculated amount of transferred charge measured by ALMO-COVP is typically much smaller than that of ETS-NOCV and explain why the ALMO-COVP values should be preferred. While the two schemes are independent, ETS-NOCV and ALMO-COVP in fact give a very similar chemical picture for a variety of chemical interactions, including H-H+, the transition structure for the Diels-Alder reaction between ethene and butadiene, and two hydrogen-bonded complexes, H2O···F- and H2O···HF.

Published
2022

21. Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells

Author

Hong, Weiqi, Yang, Jingyun, Zou, Jun, Bi, Zhenfei, He, Cai, Lei, Hong, He, Xuemei, Li, Xue, Alu, Aqu, Ren, Wenyan, Wang, Zeng, Jiang, Xiaohua, Zhong, Kunhong, Jia, Guowen, Yang, Yun, Yu, Wenhai, Huang, Qing, Yang, Mengli, Zhou, Yanan, Zhao, Yuan, Kuang, Dexuan, Wang, Junbin, Wang, Haixuan, Chen, Siyuan, Luo, Min, Zhang, Ziqi, Lu, Tianqi, Chen, Li, Que, Haiying, He, Zhiyao, Sun, Qiu, Wang, Wei, Shen, Guobo, Lu, Guangwen, Zhao, Zhiwei, Yang, Li, Yang, Jinliang, Wang, Zhenling, Li, Jiong, Song, Xiangrong, Dai, Lunzhi, Chen, Chong, Geng, Jia, Gou, Maling, Chen, Lu, Dong, Haohao, Peng, Yong, Huang, Canhua, Qian, Zhiyong, Cheng, Wei, Fan, Changfa, Wei, Yuquan, Su, Zhaoming, Tong, Aiping, Lu, Shuaiyao, Peng, Xiaozhong, and Wei, Xiawei

Published
2022
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27. A self-assembled trimeric protein vaccine induces protective immunity against Omicron variant

Author

He, Cai, Yang, Jingyun, Hong, Weiqi, Chen, Zimin, Peng, Dandan, Lei, Hong, Alu, Aqu, He, Xuemei, Bi, Zhenfei, Jiang, Xiaohua, Jia, Guowen, Yang, Yun, Zhou, Yanan, Yu, Wenhai, Tang, Cong, Huang, Qing, Yang, Mengli, Li, Bai, Li, Jingmei, Wang, Junbin, Que, Haiying, Chen, Li, Ren, Wenyan, Wan, Dandan, Li, Jiong, Wang, Wei, Shen, Guobo, Zhao, Zhiwei, Yang, Li, Yang, Jinliang, Wang, Zhenling, Su, Zhaoming, Wei, Yuquan, Cen, Xiaobo, Tanaka, Yoshimasa, Song, Xiangrong, Lu, Shuaiyao, Peng, Xiaozhong, Lu, Guangwen, and Wei, Xiawei

Published
2022
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28. Recruitment of USP10 by GCS1 to deubiquitinate GRP78 promotes the progression of colorectal cancer via alleviating endoplasmic reticulum stress.

Author

Chen, Yang, Shen, Hengyang, Wang, Zhenling, Huang, Changzhi, Zhang, Hongqiang, Shao, Yu, Tong, Ying, Xu, Lei, Lu, Yunfei, and Fu, Zan

Subjects
GENE expression, BIOLOGICAL assay, ENDOPLASMIC reticulum, COLORECTAL cancer, PROTEIN expression
Abstract

Background: Long-term accumulation of misfolded proteins leads to endoplasmic reticulum (ER) stress in colorectal cancer (CRC). However, the precise pathways controlling the decision between survival and apoptosis in CRC are unclear. Therefore, in this study, we investigated the function and molecular mechanism of glucosidase I (GCS1) in regulating ER stress in CRC. Methods: A public database was used to confirm the expression level of GCS1 in CRC and normal tissues. Clinical samples from our center were used to confirm the mRNA and protein expression levels of GCS1. Cell proliferation, migration, invasion, and apoptosis assays revealed the biological role of GCS1. Immunohistochemical techniques were used to evaluate the expression of key proteins in subcutaneous implanted tumors in nude mice, which provided further evidence for the biological function of GCS1 in promoting cancer in vivo. The results of coimmunoprecipitation-mass spectrometry analysis and immunofluorescence colocalization analysis the interaction between GCS1 and GRP78. In addition, the mechanism of action of USP10, GRP78, and GCS1 at the post- translational level was investigated. Finally, a tissue microarray was used to examine the connection between GCS1 and GRP78 expression and intracellular localization of these proteins using immunohistochemistry and immunofluorescence. Results: The experimental results revealed that GCS1 was substantially expressed in CRC, with higher expression indicating a worse prognosis. Thus, GCS1 can enhance the proliferation and metastasis while inhibiting the apoptosis of CRC cells both in vivo and in vitro. Mechanistically, GCS1 binds to GRP78, recruits USP10 for deubiquitination of GRP78 to promote its degradation, and decreases ER stress-mediated apoptosis, increasing CRC cell proliferation and metastasis. Conclusions: In summary, GCS1 stimulates CRC growth and migration and reduces ER stress-mediated apoptosis via USP10-mediated deubiquitination of GRP78. Our findings identify a possible therapeutic target for CRC. [ABSTRACT FROM AUTHOR]

Published
2024
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31. A chimeric adenovirus‐vectored vaccine based on Beta spike and Delta RBD confers a broad‐spectrum neutralization against Omicron‐included SARS‐CoV‐2 variants

Author

Hong, Weiqi, primary, Lei, Hong, additional, Peng, Dandan, additional, Huang, Yuhe, additional, He, Cai, additional, Yang, Jingyun, additional, Zhou, Yanan, additional, Liu, Jian, additional, Pan, Xiangyu, additional, Que, Haiying, additional, Alu, Aqu, additional, Chen, Li, additional, Ai, Jiayuan, additional, Qin, Furong, additional, Wang, Binhan, additional, Ao, Danyi, additional, Zeng, Zhen, additional, Hao, Ying, additional, Zhang, Yu, additional, Huang, Xiya, additional, Ye, Chunjun, additional, Fu, MinYang, additional, He, Xuemei, additional, Bi, Zhenfei, additional, Han, Xuejiao, additional, Luo, Min, additional, Hu, Hongbo, additional, Cheng, Wei, additional, Dong, Haohao, additional, Lei, Jian, additional, Chen, Lu, additional, Zhou, Xikun, additional, Wang, Wei, additional, Lu, Guangwen, additional, Shen, Guobo, additional, Yang, Li, additional, Yang, Jinliang, additional, Li, Jiong, additional, Wang, Zhenling, additional, Song, Xiangrong, additional, Sun, Qiangming, additional, Lu, Shuaiyao, additional, Wang, Youchun, additional, Cheng, Ping, additional, and Wei, Xiawei, additional

Published
2024
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40. Controlled Synthesis of Large-Area Oriented ZnO Nanoarrays.

Author

Lin, Haowei, Xing, Shibo, Jiang, Ao, Li, Mingxuan, Chen, Qing, Wang, Zhenling, Jiang, Lei, Li, Huiying, Wang, Jie, and Zhou, Chenchen

Subjects
SCANNING transmission electron microscopy, ZINC oxide, OPTOELECTRONIC devices
Abstract

Large-area oriented ZnO nanoarrays (including nanowire, nanorod, and nanotube) on ITO glass substrates are synthesized via the simple hydrothermal, electrodeposition, and electrochemical etching approach. The morphology of ZnO nanoarrays is controlled by adjusting the reaction temperature, reaction time, and current density. The scanning and transmission electron microscopy (SEM and TEM) results indicate the successful preparation of large-area oriented ZnO nanoarrays with different types, and the energy-dispersive X-microanalysis spectrum (EDS) and X-ray diffraction (XRD) results confirm that the composition of the obtained nanoarrays is ZnO. More importantly, the as-prepared ZnO nanotube arrays are observed with about a 40% increase in ultraviolet absorption intensity compared to the ZnO nanowire/nanorod arrays, due to having larger specific surface areas. The as-prepared different types of ZnO nanoarrays have great potential for applications in low-cost and high-performance optoelectronic devices. [ABSTRACT FROM AUTHOR]

Published
2024
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47. A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity

Author

Yang, Jingyun, Wang, Wei, Chen, Zimin, Lu, Shuaiyao, Yang, Fanli, Bi, Zhenfei, Bao, Linlin, Mo, Fei, Li, Xue, Huang, Yong, Hong, Weiqi, Yang, Yun, Zhao, Yuan, Ye, Fei, Lin, Sheng, Deng, Wei, Chen, Hua, Lei, Hong, Zhang, Ziqi, Luo, Min, Gao, Hong, Zheng, Yue, Gong, Yanqiu, Jiang, Xiaohua, Xu, Yanfeng, Lv, Qi, Li, Dan, Wang, Manni, Li, Fengdi, Wang, Shunyi, Wang, Guanpeng, Yu, Pin, Qu, Yajin, Yang, Li, Deng, Hongxin, Tong, Aiping, Li, Jiong, Wang, Zhenling, Yang, Jinliang, Shen, Guobo, Zhao, Zhiwei, Li, Yuhua, Luo, Jingwen, Liu, Hongqi, Yu, Wenhai, Yang, Mengli, Xu, Jingwen, Wang, Junbin, Li, Haiyan, Wang, Haixuan, Kuang, Dexuan, Lin, Panpan, Hu, Zhengtao, Guo, Wei, Cheng, Wei, He, Yanlin, Song, Xiangrong, Chen, Chong, Xue, Zhihong, Yao, Shaohua, Chen, Lu, Ma, Xuelei, Chen, Siyuan, Gou, Maling, Huang, Weijin, Wang, Youchun, Fan, Changfa, Tian, Zhixin, Shi, Ming, Wang, Fu-Sheng, Dai, Lunzhi, Wu, Min, Li, Gen, Wang, Guangyu, Peng, Yong, Qian, Zhiyong, Huang, Canhua, Lau, Johnson Yiu-Nam, Yang, Zhenglin, Wei, Yuquan, Cen, Xiaobo, Peng, Xiaozhong, Qin, Chuan, Zhang, Kang, Lu, Guangwen, and Wei, Xiawei

Published
2020
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