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182 results on '"Wang, Zack Z."'

2. Gene and protein expression in human megakaryocytes derived from induced pluripotent stem cells

Author

Kammers, Kai, Taub, Margaret A., Mathias, Rasika A., Yanek, Lisa R., Kanchan, Kanika, Venkatraman, Vidya, Sundararaman, Niveda, Martin, Joshua, Liu, Senquan, Hoyle, Dixie, Raedschelders, Koen, Holewinski, Ronald, Parker, Sarah, Dardov, Victoria, Faraday, Nauder, Becker, Diane M., Cheng, Linzhao, Wang, Zack Z., Leek, Jeffrey T., Van Eyk, Jennifer E., and Becker, Lewis C.

Published
2021
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7. PEAR1 is a potential regulator of early hematopoiesis of human pluripotent stem cells.

Author

Shuo Zhang, Kengyuan Qu, Shuzhen Lyu, Hoyle, Dixie L., Smith, Cory, Linzhao Cheng, Tao Cheng, Jun Shen, and Wang, Zack Z.

Subjects
PLURIPOTENT stem cells, HUMAN stem cells, BONE morphogenetic proteins, HEMATOPOIESIS, FIBRONECTINS, PROGENITOR cells, BONE morphogenetic protein receptors, BLOOD platelet aggregation
Abstract

Hemogenic endothelial (HE) cells are specialized endothelial cells to give rise to hematopoietic stem/progenitor cells during hematopoietic development. The underlying mechanisms that regulate endothelial‐to‐hematopoietic transition (EHT) of human HE cells are not fully understand. Here, we identified platelet endothelial aggregation receptor‐1 (PEAR1) as a novel regulator of early hematopoietic development in human pluripotent stem cells (hPSCs). We found that the expression of PEAP1 was elevated during hematopoietic development. A subpopulation of PEAR1+ cells overlapped with CD34+ CD144+ CD184+ CD73− arterial‐type HE cells. Transcriptome analysis by RNA sequencing indicated that TAL1/SCL, GATA2, MYB, RUNX1 and other key transcription factors for hematopoietic development were mainly expressed in PEAR1+ cells, whereas the genes encoding for niche‐related signals, such as fibronectin, vitronectin, bone morphogenetic proteins and jagged1, were highly expressed in PEAR1− cells. The isolated PEAR1+ cells exhibited significantly greater EHT capacity on endothelial niche, compared with the PEAR1− cells. Colony‐forming unit (CFU) assays demonstrated the multilineage hematopoietic potential of PEAR1+ ‐derived hematopoietic cells. Furthermore, PEAR1 knockout in hPSCs by CRISPR/Cas9 technology revealed that the hematopoietic differentiation was impaired, resulting in decreased EHT capacity, decreased expression of hematopoietic‐related transcription factors, and increased expression of niche‐ related signals. In summary, this study revealed a novel role of PEAR1 in balancing intrinsic and extrinsic signals for early hematopoietic fate decision. [ABSTRACT FROM AUTHOR]

Published
2023
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13. Single-cell transcriptome of early hematopoiesis guides arterial endothelial-enhanced functional T cell generation from human PSCs

Author

Shen, Jun, primary, Xu, Yingxi, additional, Zhang, Shuo, additional, Lyu, Shuzhen, additional, Huo, Yingying, additional, Zhu, Yaoyao, additional, Tang, Kejing, additional, Mou, Junli, additional, Li, Xinjie, additional, Hoyle, Dixie L., additional, Wang, Min, additional, Wang, Jianxiang, additional, Li, Xin, additional, Wang, Zack Z., additional, and Cheng, Tao, additional

Published
2021
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17. Cover Image

Author

Shen, Jun, primary, Zhu, Yaoyao, additional, Zhang, Shuo, additional, Lyu, Shuzhen, additional, Lyu, Cuicui, additional, Feng, Zicen, additional, Hoyle, Dixie L., additional, Wang, Zack Z., additional, and Cheng, Tao, additional

Published
2021
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21. Sequential cellular niches control the generation of enucleated erythrocytes from human pluripotent stem cells

Author

Shen, Jun, primary, Zhu, Yaoyao, additional, Lyu, Cuicui, additional, Feng, Zicen, additional, Lyu, Shuzhen, additional, Zhao, Yuping, additional, Hoyle, Dixie L., additional, Ji, Guangzhen, additional, Miao, Weimin, additional, Zhang, Xiaobing, additional, Cheng, Linzhao, additional, Brodsky, Robert A., additional, Cheng, Tao, additional, and Wang, Zack Z., additional

Published
2019
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24. Integrity of Induced Pluripotent Stem Cell (iPSC) Derived Megakaryocytes as Assessed by Genetic and Transcriptomic Analysis

Author

Kammers, Kai, primary, Taub, Margaret A., additional, Ruczinski, Ingo, additional, Martin, Joshua, additional, Yanek, Lisa R., additional, Frazee, Alyssa, additional, Gao, Yongxing, additional, Hoyle, Dixie, additional, Faraday, Nauder, additional, Becker, Diane M., additional, Cheng, Linzhao, additional, Wang, Zack Z., additional, Leek, Jeff T., additional, Becker, Lewis C., additional, and Mathias, Rasika A., additional

Published
2017
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25. Definitive Hematopoietic Multipotent Progenitor Cells Are Transiently Generated From Hemogenic Endothelial Cells in Human Pluripotent Stem Cells

Author

BAI, HAO, LIU, YANFENG, XIE, YINLIANG, HOYLE, DIXIE L., BRODSKY, ROBERT A., CHENG, LINZHAO, CHENG, TAO, and WANG, ZACK Z.

Subjects
Pluripotent Stem Cells, Erythroid Cells, Antigens, CD, Multipotent Stem Cells, Endothelial Cells, Humans, Intercellular Signaling Peptides and Proteins, Cell Differentiation, Cell Lineage, Article, Cell Line, Hematopoiesis
Abstract

Generation of fully functional hematopoietic multipotent progenitor (MPP) cells from human pluripotent stem cells (hPSCs) has a great therapeutic potential to provide an unlimited cell source for treatment of hematological disorders. We previously demonstrated that CD34(+) CD31(+) CD144(+) population derived from hPSCs contain hemato-endothelial progenitors (HEPs) that give rise to hematopoietic and endothelial cells. Here, we report a differentiation system to generate definitive hematopoietic MPP cells from HEPs via endothelial monolayer. In the presence of angiogenic factors, HEPs formed an endothelial monolayer, from which hematopoietic clusters emerged through the process of endothelial-to-hematopoietic transition (EHT). EHT was significantly enhanced by hematopoietic growth factors. The definitive MPP cells generated from endothelial monolayer were capable of forming multilineage hematopoietic colonies, giving rise to T lymphoid cells, and differentiating into enucleated erythrocytes. Emergence of hematopoietic cells from endothelial monolayer occurred transiently. Hematopoietic potential was lost during prolonged culture of HEPs in endothelial growth conditions. Our study demonstrated that CD34(+) CD31(+) CD144(+) HEPs gave rise to hematopoietic MPP cells via hemogenic endothelial cells that exist transiently. The established differentiation system provides a platform for future investigation of regulatory factors involved in de novo generation of hematopoietic MPP cells and their applications in transplantation.

Published
2015

40. Definitive Hematopoietic Multipotent Progenitor Cells Are Transiently Generated From Hemogenic Endothelial Cells in Human Pluripotent Stem Cells.

Author

Bai, Hao, Liu, Yanfeng, Xie, Yinliang, Hoyle, Dixie L., Brodsky, Robert A., Cheng, Linzhao, Cheng, Tao, and Wang, Zack Z.

Subjects
REJUVENESCENCE (Botany), CYTOPROTECTION, VASCULAR endothelial growth factors, ERYTHROCYTES, BLOOD cells
Abstract

Generation of fully functional hematopoietic multipotent progenitor (MPP) cells from human pluripotent stem cells (hPSCs) has a great therapeutic potential to provide an unlimited cell source for treatment of hematological disorders. We previously demonstrated that CD34+CD31+CD144+ population derived from hPSCs contain hemato-endothelial progenitors (HEPs) that give rise to hematopoietic and endothelial cells. Here, we report a differentiation system to generate definitive hematopoietic MPP cells from HEPs via endothelial monolayer. In the presence of angiogenic factors, HEPs formed an endothelial monolayer, from which hematopoietic clusters emerged through the process of endothelial-to-hematopoietic transition (EHT). EHT was significantly enhanced by hematopoietic growth factors. The definitive MPP cells generated from endothelial monolayer were capable of forming multilineage hematopoietic colonies, giving rise to T lymphoid cells, and differentiating into enucleated erythrocytes. Emergence of hematopoietic cells from endothelial monolayer occurred transiently. Hematopoietic potential was lost during prolonged culture of HEPs in endothelial growth conditions. Our study demonstrated that CD34+CD31+CD144+ HEPs gave rise to hematopoietic MPP cells via hemogenic endothelial cells that exist transiently. The established differentiation system provides a platform for future investigation of regulatory factors involved in de novo generation of hematopoietic MPP cells and their applications in transplantation. J. Cell. Physiol. 231: 1065-1076, 2016. © 2015 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

Published
2016
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