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156 results on '"Wang, Xuya"'

8. Nivolumab with or without ipilimumab in patients with recurrent or metastatic cervical cancer (CheckMate 358): a phase 1–2, open-label, multicohort trial

Author

Oaknin, Ana, Moore, Kathleen, Meyer, Tim, López-Picazo González, José, Devriese, Lot A, Amin, Asim, Lao, Christopher D, Boni, Valentina, Sharfman, William H, Park, Jong Chul, Tahara, Makoto, Topalian, Suzanne L, Magallanes, Manuel, Molina Alavez, Alejandro, Khan, Tariq Aziz, Copigneaux, Catherine, Lee, Michelle, Garnett-Benson, Charlie, Wang, Xuya, and Naumann, R Wendel

Published
2024
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18. Nivolumab with or without ipilimumab in patients with recurrent or metastatic cervical cancer (CheckMate 358): a phase 1-2, open-label, multicohort trial

Author

MS Medische Oncologie, Cancer, Oaknin, Ana, Moore, Kathleen, Meyer, Tim, López-Picazo González, José, Devriese, Lot A, Amin, Asim, Lao, Christopher D, Boni, Valentina, Sharfman, William H, Park, Jong Chul, Tahara, Makoto, Topalian, Suzanne L, Magallanes, Manuel, Molina Alavez, Alejandro, Khan, Tariq Aziz, Copigneaux, Catherine, Lee, Michelle, Garnett-Benson, Charlie, Wang, Xuya, Naumann, R Wendel, MS Medische Oncologie, Cancer, Oaknin, Ana, Moore, Kathleen, Meyer, Tim, López-Picazo González, José, Devriese, Lot A, Amin, Asim, Lao, Christopher D, Boni, Valentina, Sharfman, William H, Park, Jong Chul, Tahara, Makoto, Topalian, Suzanne L, Magallanes, Manuel, Molina Alavez, Alejandro, Khan, Tariq Aziz, Copigneaux, Catherine, Lee, Michelle, Garnett-Benson, Charlie, Wang, Xuya, and Naumann, R Wendel

Published
2024

20. “Perfect” designer chromosome V and behavior of a ring derivative

Author

Xie, Ze-Xiong, Li, Bing-Zhi, Mitchell, Leslie A., Wu, Yi, Qi, Xin, Jin, Zhu, Jia, Bin, Wang, Xia, Zeng, Bo-Xuan, Liu, Hui-Min, Wu, Xiao-Le, Feng, Qi, Zhang, Wen-Zheng, Liu, Wei, Ding, Ming-Zhu, Li, Xia, Zhao, Guang-Rong, Qiao, Jian-Jun, Cheng, Jing-Sheng, Zhao, Meng, Kuang, Zheng, Wang, Xuya, Martin, J. Andrew, Stracquadanio, Giovanni, Yang, Kun, Bai, Xue, Zhao, Juan, Hu, Meng-Long, Lin, Qiu-Hui, Zhang, Wen-Qian, Shen, Ming-Hua, Chen, Si, Su, Wan, Wang, En-Xu, Guo, Rui, Zhai, Fang, Guo, Xue-Jiao, Du, Hao-Xing, Zhu, Jia-Qing, Song, Tian-Qing, Dai, Jun-Jun, Li, Fei-Fei, Jiang, Guo-Zhen, Han, Shi-Lei, Liu, Shi-Yang, Yu, Zhi-Chao, Yang, Xiao-Na, Chen, Ken, Hu, Cheng, Li, Da-Shuai, Jia, Nan, Liu, Yue, Wang, Lin-Ting, Wang, Su, Wei, Xiao-Tong, Fu, Mei-Qing, Qu, Lan-Meng, Xin, Si-Yu, Liu, Ting, Tian, Kai-Ren, Li, Xue-Nan, Zhang, Jin-Hua, Song, Li-Xiang, Liu, Jin-Gui, Lv, Jia-Fei, Xu, Hang, Tao, Ran, Wang, Yan, Zhang, Ting-Ting, Deng, Ye-Xuan, Wang, Yi-Ran, Li, Ting, Ye, Guang-Xin, Xu, Xiao-Ran, Xia, Zheng-Bao, Zhang, Wei, Yang, Shi-Lan, Liu, Yi-Lin, Ding, Wen-Qi, Liu, Zhen-Ning, Zhu, Jun-Qi, Liu, Ning-Zhi, Walker, Roy, Luo, Yisha, Wang, Yun, Shen, Yue, Yang, Huanming, Cai, Yizhi, Ma, Ping-Sheng, Zhang, Chun-Ting, Bader, Joel S., Boeke, Jef D., and Yuan, Ying-Jin

Published
2017

21. Bug mapping and fitness testing of chemically synthesized chromosome X

Author

Wu, Yi, Li, Bing-Zhi, Zhao, Meng, Mitchell, Leslie A., Xie, Ze-Xiong, Lin, Qiu-Hui, Wang, Xia, Xiao, Wen-Hai, Wang, Ying, Zhou, Xiao, Liu, Hong, Li, Xia, Ding, Ming-Zhu, Liu, Duo, Zhang, Lu, Liu, Bao-Li, Wu, Xiao-Le, Li, Fei-Fei, Dong, Xiu-Tao, Jia, Bin, Zhang, Wen-Zheng, Jiang, Guo-Zhen, Liu, Yue, Bai, Xue, Song, Tian-Qing, Chen, Yan, Zhou, Si-Jie, Zhu, Rui-Ying, Gao, Feng, Kuang, Zheng, Wang, Xuya, Shen, Michael, Yang, Kun, Stracquadanio, Giovanni, Richardson, Sarah M., Lin, Yicong, Wang, Lihui, Walker, Roy, Luo, Yisha, Ma, Ping-Sheng, Yang, Huanming, Cai, Yizhi, Dai, Junbiao, Bader, Joel S., Boeke, Jef D., and Yuan, Ying-Jin

Published
2017

22. Synthesis, debugging, and effects of synthetic chromosome consolidation : synVI and beyond

Author

Mitchell, Leslie A., Wang, Ann, Stracquadanio, Giovanni, Kuang, Zheng, Wang, Xuya, Yang, Kun, Richardson, Sarah, Martin, J. Andrew, Zhao, Yu, Walker, Roy, Luo, Yisha, Dai, Hongjiu, Dong, Kang, Tang, Zuojian, Yang, Yanling, Cai, Yizhi, Heguy, Adriana, Ueberheide, Beatrix, Fenyö, David, Dai, Junbiao, Bader, Joel S., and Boeke, Jef D.

Published
2017

24. An Analysis of the Sensitivity of Proteogenomic Mapping of Somatic Mutations and Novel Splicing Events in Cancer

Author

Ruggles, Kelly V., Tang, Zuojian, Wang, Xuya, Grover, Himanshu, Askenazi, Manor, Teubl, Jennifer, Cao, Song, McLellan, Michael D., Clauser, Karl R., Tabb, David L., Mertins, Philipp, Slebos, Robbert, Erdmann-Gilmore, Petra, Li, Shunqiang, Gunawardena, Harsha P., Xie, Ling, Liu, Tao, Zhou, Jian-Ying, Sun, Shisheng, Hoadley, Katherine A., Perou, Charles M., Chen, Xian, Davies, Sherri R., Maher, Christopher A., Kinsinger, Christopher R., Rodland, Karen D., Zhang, Hui, Zhang, Zhen, Ding, Li, Townsend, R. Reid, Rodriguez, Henry, Chan, Daniel, Smith, Richard D., Liebler, Daniel C., Carr, Steven A., Payne, Samuel, Ellis, Matthew J., and Fenyő, David

Published
2016
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25. LINE-1 ORF2p expression is nearly imperceptible in human cancers

Author

Ardeljan, Daniel, Wang, Xuya, Oghbaie, Mehrnoosh, Taylor, Martin S., Husband, David, Deshpande, Vikram, Steranka, Jared P., Gorbounov, Mikhail, Yang, Wan Rou, Sie, Brandon, Larman, H. Benjamin, Jiang, Hua, Molloy, Kelly R., Altukhov, Ilya, Li, Zhi, McKerrow, Wilson, Fenyö, David, Burns, Kathleen H., and LaCava, John

Published
2019
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26. Supplementary File 5 from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
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27. Supplementary File 1 from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
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28. Supplementary File 2 from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
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29. Extended Methods from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
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30. Supplementary File 3 from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
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31. Supplementary Figures from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
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32. Supplementary tables 1 and 2 from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
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33. Data from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
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34. Supplementary File 4 from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
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36. China Artificial Intelligence Industry Insights

Author

Ebert, Christof and Wang, Xuya

Abstract

You may use Alipay, Baidu, or TikTok. But did you know that Chinese AI and IT increasingly influence everyday life? The rapid growth of tech business in China has contributed to the development of a dynamic and competitive landscape. This article provides insights.

Published
2024
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37. PDPN contributes to constructing immunosuppressive microenvironment in IDH wildtype glioma

Author

Wang, Xuya, primary, Wang, Xisen, additional, Li, Jiabo, additional, Liang, Jianshen, additional, Ren, Xiao, additional, Yun, Debo, additional, Liu, Jie, additional, Fan, Jikang, additional, Zhang, Yiming, additional, Zhang, Jinhao, additional, Ren, Xiude, additional, Zhang, Hao, additional, Shang, Guanjie, additional, Sun, Jinzhang, additional, Chen, Lulu, additional, Chen, Lei, additional, Li, Tao, additional, Tong, Luqing, additional, Zhang, Chen, additional, Yu, Shengping, additional, and Yang, Xuejun, additional

Published
2022
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39. Nivolumab plus rucaparib for metastatic castration-resistant prostate cancer: results from the phase 2 CheckMate 9KD trial

Author

Fizazi, Karim, primary, Retz, Margitta, additional, Petrylak, Daniel P, additional, Goh, Jeffrey C, additional, Perez-Gracia, Jose, additional, Lacombe, Louis, additional, Zschäbitz, Stefanie, additional, Burotto, Mauricio, additional, Mahammedi, Hakim, additional, Gravis, Gwenaelle, additional, Bastos, Diogo Assed, additional, McCune, Steven L, additional, Vázquez Limón, Juan Carlos, additional, Kwan, Edmond M, additional, Castellano, Daniel, additional, Fléchon, Aude, additional, Saad, Fred, additional, Grimm, Marc-Oliver, additional, Shaffer, David R, additional, Armstrong, Andrew J, additional, Bhagavatheeswaran, Prabhu, additional, Amin, Neha P, additional, Ünsal-Kaçmaz, Keziban, additional, Wang, Xuya, additional, Li, Jun, additional, Loehr, Andrea, additional, and Pachynski, Russell K, additional

Published
2022
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40. Abstract CT023: Nivolumab (NIVO) plus chemotherapy (chemo) vs chemo as first-line (1L) treatment for advanced gastric cancer/gastroesophageal junction cancer/esophageal adenocarcinoma (GC/GEJC/EAC): CheckMate 649 biomarker analyses

Author

Lei, Ming, primary, Janjigian, Yelena Y., additional, Ajani, Jaffer A., additional, Moehler, Markus, additional, Wang, Xuya, additional, Shen, Lin, additional, Garrido, Marcelo, additional, Gallardo, Carlos, additional, Yamaguchi, Kensei, additional, Wyrwicz, Lucjan, additional, Skoczylas, Tomasz, additional, Bragagnoli, Arinilda, additional, Liu, Tianshu, additional, Tehfe, Mustapha, additional, Elimova, Elena, additional, Li, Mingshun, additional, Poulart, Valerie, additional, Wang, Yu, additional, Doshi, Parul, additional, and Shitara, Kohei, additional

Published
2022
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41. A Novel Prognostic Signature Based on Glioma Essential Ferroptosis-Related Genes Predicts Clinical Outcomes and Indicates Treatment in Glioma

Author

Yun, Debo, primary, Wang, Xuya, additional, Wang, Wenbo, additional, Ren, Xiao, additional, Li, Jiabo, additional, Wang, Xisen, additional, Liang, Jianshen, additional, Liu, Jie, additional, Fan, Jikang, additional, Ren, Xiude, additional, Zhang, Hao, additional, Shang, Guanjie, additional, Sun, Jingzhang, additional, Chen, Lei, additional, Li, Tao, additional, Zhang, Chen, additional, Yu, Shengping, additional, and Yang, Xuejun, additional

Published
2022
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42. Correction: Corrigendum: Proteogenomic integration reveals therapeutic targets in breast cancer xenografts

Author

Huang, Kuan-lin, Li, Shunqiang, Mertins, Philipp, Cao, Song, Gunawardena, Harsha P., Ruggles, Kelly V., Mani, D. R., Clauser, Karl R., Tanioka, Maki, Usary, Jerry, Kavuri, Shyam M., Xie, Ling, Yoon, Christopher, Qiao, Jana W., Wrobel, John, Wyczalkowski, Matthew A., Erdmann-Gilmore, Petra, Snider, Jacqueline E., Hoog, Jeremy, Singh, Purba, Niu, Beifang, Guo, Zhanfang, Sun, Sam Qiancheng, Sanati, Souzan, Kawaler, Emily, Wang, Xuya, Scott, Adam, Ye, Kai, McLellan, Michael D., Wendl, Michael C., Malovannaya, Anna, Held, Jason M., Gillette, Michael A., Fenyö, David, Kinsinger, Christopher R., Mesri, Mehdi, Rodriguez, Henry, Davies, Sherri R., Perou, Charles M., Ma, Cynthia, Townsend, R. Reid, Chen, Xian, Carr, Steven A., Ellis, Matthew J., and Ding, Li

Published
2017
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45. Comprehensive Scanning Mutagenesis of Human Retrotransposon LINE-1 Identifies Motifs Essential for Function

Author

Adney, Emily M., Ochmann, Matthias T., Sil, Srinjoy, Truong, David M., Mita, Paolo, Wang, Xuya, Kahler, David J., Fenyö, David, Holt, Liam J., and Boeke, Jef D.

Subjects
Models, Molecular, Transposable element, Retroelements, Mutant, Locus (genetics), Retrotransposon, Computational biology, Investigations, Biology, LINE-1, Open Reading Frames, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Cellular Genetics, Protein Domains, scanning mutagenesis, Genetics, Humans, Amino Acid Sequence, Nucleotide Motifs, Conserved Sequence, 030304 developmental biology, 0303 health sciences, Base Sequence, retrotransposon, RNA, L1, Reverse transcriptase, 3. Good health, Long Interspersed Nucleotide Elements, chemistry, Mutagenesis, Mutation, Human genome, Cell Nucleolus, 030217 neurology & neurosurgery, DNA, HeLa Cells
Abstract

Adney et al. describe the complete and comprehensive codon substitution mutagenesis of human retrotransposon LINE-1 using a synthetic DNA approach. This experiment is the first of its kind for any transposon..., Long Interspersed Nuclear Element-1 (LINE-1, L1) is the only autonomous active transposable element in the human genome. The L1-encoded proteins ORF1p and ORF2p enable the element to jump from one locus to another via a “copy-and-paste” mechanism. ORF1p is an RNA-binding protein, and ORF2p has endonuclease and reverse transcriptase activities. The huge number of truncated L1 remnants in the human genome suggests that the host has likely evolved mechanisms to prevent full L1 replication, and thereby decrease the proliferation of active elements and reduce the mutagenic potential of L1. In turn, L1 appears to have a minimized length to increase the probability of successful full-length replication. This streamlining would be expected to lead to high information density. Here, we describe the construction and initial characterization of a library of 538 consecutive trialanine substitutions that scan along ORF1p and ORF2p to identify functionally important regions. In accordance with the streamlining hypothesis, retrotransposition was overall very sensitive to mutations in ORF1p and ORF2p; only 16% of trialanine mutants retained near-wild-type (WT) activity. All ORF1p mutants formed near-WT levels of mRNA transcripts and 75% formed near-WT levels of protein. Two ORF1p mutants presented a unique nucleolar-relocalization phenotype. Regions of ORF2p that are sensitive to mutagenesis but lack phylogenetic conservation were also identified. We provide comprehensive information on the regions most critical to retrotransposition. This resource will guide future studies of intermolecular interactions that form with RNA, proteins, and target DNA throughout the L1 life cycle.

Published
2019
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47. CheckMate 9KD cohort A1 final analysis: Nivolumab (NIVO) + rucaparib for post-chemotherapy (CT) metastatic castration-resistant prostate cancer (mCRPC).

Author

Pachynski, Russell Kent, primary, Retz, Margitta, additional, Goh, Jeffrey C., additional, Burotto, Mauricio, additional, Gravis, Gwenaelle, additional, Castellano, Daniel, additional, Flechon, Aude, additional, Zschaebitz, Stefanie, additional, Shaffer, David R., additional, Vazquez Limon, Juan Carlos, additional, Grimm, Marc-Oliver, additional, McCune, Steven L., additional, Amin, Neha P., additional, Li, Jia, additional, Wang, Xuya, additional, Unsal-Kacmaz, Keziban, additional, Saad, Fred, additional, Petrylak, Daniel P., additional, and Fizazi, Karim, additional

Published
2021
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48. Complementary detection of genomic alterations in metastatic castration-resistant prostate cancer (mCRPC) from CheckMate 9KD through analyses of tumor tissue and plasma DNA.

Author

Sausen, Mark, primary, Baden, Jonathan F., additional, Kalinava, Natallia, additional, Wang, Xuya, additional, Li, Jun, additional, Anguiano, Esperanza, additional, Unsal-Kacmaz, Keziban, additional, Wind-Rotolo, Megan, additional, Quinn, Katie, additional, and Green, George, additional

Published
2021
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