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[Objectives] To our knowledge, little is known about its molecular biological functions in Camellia sinensis. [Methods] The Open reading frame (ORF) of CsIISP17.2 gene was isolated from C. sinensis 'Yingshuang'. The obtained ORF sequence and deduced amino acid sequence; was analysed with corresponding bioinformatic softwares. The; real-time; PCR was performed to stuely the; expression pre)file; of CsHSP17.2 gem;. [ Results] The ORF of CsHSP17.2 was 453 bp, encoding 150 amino acids. Bioinformatic; analysis showed that the; relative melecular weight of the; predicted prertein was 17.2x 103 and the; theoretic isoelectricpeint was 5.56. Besides, the predicted protein had no signal peptide, belonging to nemsecretory protein. Subcellular localization indicated that CsIISP17.2 protein was distributed in cytoplasm. Poylogenetic tree; analysis showed that CsIISPI7.2 had a close; genetic; relationship with Oryza sativa (GenBank accession No. ;P27777)and Arachis hypogaea( ABC41131) , which was grouped to subfamily Class I. Real-time; quantitative PCR found that CsHSP17.2 gene; was expressed in various organs(roerts, stems, leaves, buds, flowers and fruits), and also revealed that this gene; could be; significantly induced by high temperature(P<0.05). Besiedes, the relative; transcript accumulation of CsHSP17.2 was up-regulated by drought, high salinity and ABA treatment at certain levels. [Conclusions] CsHSP17. 2 gene; from C. sinensis 'Yingshuang' was isolated. This gene was up-regulated by high temperature, which provided evidence for heat tolerence of tea plant. [ABSTRACT FROM AUTHOR]

While spent mushroom substrate (SMS) has shown promise in increasing soil organic carbon (SOC) and improving soil quality, research on the interplay between SOC components and microbial community following the application of diverse SMS types remains scant. A laboratory soil incubation experiment was conducted with application of two types of SMSs from cultivation of Pleurotus eryngii (PE) and Agaricus bisporus (AB), each at three application rates (3, 5.5, and 8%). Advanced techniques, including solid-state 13 C nuclear magnetic resonance (NMR) and high-throughput sequencing, were employed to investigate on SOC fractions and chemical structure, microbial community composition and functionality. Compared to SMS-AB, SMS-PE application increased the relative abundances of carbohydrate carbon and O-alkyl C in SOC. In addition, SMS-PE application increased the relative abundance of the bacterial phylum Proteobacteria and those of the fungal phyla Basidiomycota and Ascomycota. The relative abundances of cellulose-degrading bacterial (e.g., Flavisolibacter and Agromyces ) and fungal genera (e.g., Myceliophthora , Thermomyces , and Conocybe ) were increased as well. The application of SMS-AB increased the aromaticity index of SOC, the relative abundance of aromatic C, and the contents of humic acid and heavy fraction organic carbon. In addition, SMS-AB application significantly increased the relative abundances of the bacterial phyla Firmicutes and Actinobacteria. Notably, the genera Actinomadura , Ilumatobacter , and Bacillus , which were positively correlated with humic acid, experienced an increase in relative abundance. Functional prediction revealed that SMS-PE application elevated carbohydrate metabolism and reduced the prevalence of fungal pathogens, particularly Fusarium . The application of high-rate SMS-AB (8%) enhanced bacterial amino acid metabolism and the relative abundances of plant pathogenic fungi. Our research provides strategies for utilizing SMS to enrich soil organic carbon and fortify soil health, facilitating the achievement of sustainable soil management., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Yang, Ma, Ma, Wang, Lu, Xu, Luo and Guo.)

Objective: To benchmark opioid abuse risk among student pharmacists attending three northeast pharmacy schools utilizing the opioid risk tool (ORT)., Design: A cross-sectional, anonymous risk assessment questionnaire., Setting: Three pharmacy schools in the northeast United States., Participants: Professional year 1 (P1) through professional year 3 (P3) student pharmacists., Methods: ORT was collected and scored by investigators and inputted into an electronic format for analysis. Students voluntarily participated, and 812 surveys were completed during one course meeting time and day at each school., Results: The majority of students were in the low-risk category (n = 581, 71.6 percent). Additionally, 137 (16.9 percent) patients were categorized as moderate risk and 94 (11.6 percent) as high risk. No statistically significant differences existed when comparing risk groups across the first through third professional year student pharmacist cohorts. There were no statistically significant differences in the proportion of risk groups among the three pharmacy cohorts between low-risk versus the high-risk groups. When comparing risk groups by gender, males were found to have a statistically significant higher proportion of being classified as moderate or high risk., Conclusions: The results of this study demonstrate that there may be some student pharmacists with an increased risk for opioid abuse potential. There is potential need for education regarding opioid risk awareness and abuse prevention, which may serve as a call to action for professional school students and practitioners to understand baseline opioid abuse risk if they require chronic pain therapy.