Search

Severe human infection with influenza A(H5N6) virus was identified in China in 2014. During 2014-2020, a total of 26 cases of human infection were laboratory confirmed (1,2). Sporadic cases did [...]

Analyzing the processes and influencing factors of accretion or erosion in estuaries and tidal basins is important for coastal conservation and utilization, planning, and ecosystem maintenance. This study analyzed the process of topographic changes in Taizhou Bay, China over the past five decades by comparing bathymetric datasets from different years. The coastlines were extracted via remote sensing image interpretation, and the coastal reclamation process was analyzed. The results revealed that this bay experienced slow siltation from 1963 to 2004, which mainly occurred on mudflats and shore slopes. It turned into a feature dominated by erosion between 2004 and 2013, and erosion increased between 2013 and 2019. Coastal reclamation has occurred during every 10-year period since the 1960s. Reclamation occupied a portion of the intertidal flat, decreased the tidal prism, and changed tidal asymmetry, causing net sediment to be transported into the bay and accumulate on the intertidal flat and upper part of the subaqueous shore. The drastic reduction in sediment supply caused by the Three Gorges Dam may have been responsible for erosion from 2004 to 2019. A negative feedback process exists between tidal flat expansion and coastal reclamation. The compensatory expansion of the tidal flat is a much slower process than the advance of reclamation. [ABSTRACT FROM AUTHOR]

Kluyveromyces marxianus, a thermotolerant, fast‐growing, Crabtree‐negative yeast, is a promising chassis for the manufacture of various bioproducts. Although several genome editing tools are available for this yeast, these tools still require refinement to enable more convenient and efficient genetic modification. In this study, we engineered the K. marxianus NBRC 104275 strain by impairing the nonhomologous end joining and enhancing the homologous recombination machinery, which resulted in improved homology‐directed repair effective on homology arms of up to 40 bp in length. Additionally, we simplified the CRISPR‐Cas9 editing system by constructing a strain for integrative expression of Cas9 nuclease and plasmids bearing different selection markers for gRNA expression, thereby facilitating iterative genome editing without the need for plasmid curing. We demonstrated that tRNA was more effective than the hammerhead ribozyme for processing gRNA primary transcripts, and readily assembled tRNA‐gRNA arrays were used for multiplexed editing of at least four targets. This editing tool was further employed for simultaneous scarless in vivo assembly of a 12‐kb cassette from three fragments and marker‐free integration for expressing a fusion variant of fatty acid synthase, as well as the integration of genes for starch hydrolysis. Together, the genome editing tool developed in this study makes K. marxianus more amenable to genetic modification and will facilitate more extensive engineering of this nonconventional yeast for chemical production. [ABSTRACT FROM AUTHOR]

Urban management and planning during public health emergencies, such as the COVID-19 pandemic, are critical. This article examines hospital site selection in Yinzhou, Ningbo, China, using geographical data analysis. It evaluates travel costs and referral patterns between hospitals and community health facilities to establish a sustainable hierarchical diagnosis and treatment system. The results reveal that existing medical facilities, particularly in rural areas, are inadequate during public health emergencies. The article proposes considering total transportation costs in allocating the sites of new hospitals and suggests adopting a 'permanent + temporary' facility planning approach to ease financial burdens. Addressing these issues can mitigate the inequitable distribution of urban and rural medical resources. This article was published open access under a CC BY licence: https://creativecommons.org/licenses/by/4.0/. [ABSTRACT FROM AUTHOR]