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28 results on '"Wang, Shun-chang"'

16. Phospho-Priming Confers Functionally Relevant Specificities for Rad53 Kinase Autophosphorylation

Author

Chen, Eric Sheng-Wen, primary, Weng, Jui-Hung, additional, Chen, Yu-Hou, additional, Wang, Shun-Chang, additional, Liu, Xiao-Xia, additional, Huang, Wei-Cheng, additional, Matsui, Tsutomu, additional, Kawano, Yoshiaki, additional, Liao, Jiahn-Haur, additional, Lim, Liang-Hin, additional, Bessho, Yoshitaka, additional, Huang, Kai-Fa, additional, Wu, Wen-Jin, additional, and Tsai, Ming-Daw, additional

Published
2017
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17. Phospho-Priming Confers Functionally Relevant Specificities for Rad53 Kinase Autophosphorylation

Author

Chen, Eric Sheng-Wen, Weng, Jui-Hung, Chen, Yu-Hou, Wang, Shun-Chang, Liu, Xiao-Xia, Huang, Wei-Cheng, Matsui, Tsutomu, Kawano, Yoshiaki, Liao, Jiahn-Haur, Lim, Liang-Hin, Bessho, Yoshitaka, Huang, Kai-Fa, Wu, Wen-Jin, and Tsai, Ming-Daw

Abstract

The vast majority of in vitrostructural and functional studies of the activation mechanism of protein kinases use the kinase domain alone. Well-demonstrated effects of regulatory domains or allosteric factors are scarce for serine/threonine kinases. Here we use a site-specifically phosphorylated SCD1-FHA1-kinase three-domain construct of the serine/threonine kinase Rad53 to show the effect of phospho-priming, an in vivoregulatory mechanism, on the autophosphorylation intermediate and specificity. Unphosphorylated Rad53 is a flexible monomer in solution but is captured in an asymmetric enzyme:substrate complex in crystal with the two FHA domains separated from each other. Phospho-priming induces formation of a stable dimer via intermolecular pT-FHA binding in solution. Importantly, autophosphorylation of unprimed and phospho-primed Rad53 produced predominantly inactive pS350-Rad53 and active pT354-Rad53, respectively. The latter mechanism was also demonstrated in vivo. Our results show that, while Rad53 can display active conformations under various conditions, simulation of in vivoregulatory conditions confers functionally relevant autophosphorylation.

Published
2024
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21. Intermolecular Binding between TIFA-FHA and TIFA-pT Mediates Tumor Necrosis Factor Alpha Stimulation and NF-κB Activation

Author

Huang, Chia-Chi Flora, primary, Weng, Jui-Hung, additional, Wei, Tong-You Wade, additional, Wu, Pei-Yu Gabriel, additional, Hsu, Pang-Hung, additional, Chen, Yu-Hou, additional, Wang, Shun-Chang, additional, Qin, Dongyan, additional, Hung, Chin-Chun, additional, Chen, Shui-Tsung, additional, Wang, Andrew H.-J., additional, Shyy, John Y.-J., additional, and Tsai, Ming-Daw, additional

Published
2012
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27. [Analysis and assessment of atmospheric pollution based on accumulation characterization of heavy metals in Platanus acerifolia leaves].

Author

Liu L, Fang YM, Wang SC, Xie Y, and Wang CR

Subjects
China, Cities, Air Pollutants analysis, Environmental Monitoring, Magnoliopsida chemistry, Metals, Heavy analysis, Plant Leaves chemistry
Abstract

The present work was aimed to evaluate the heavy metal pollution in the atmosphere of Huainan City. We measured and clustered the accumulation of six heavy metals in Platanus acerifolia leaves in 20 sampling fields with six types of environmental conditions, and analyzed the EF value of heavy metal enrichment in the leaves. The results showed that the accumulations in Platanus acerifolia leaves varied according to different types of metals, following the order of Zn > Cu > Cr > Ni > Pb > Cd. Environmental conditions also had great influence on the accumulation of heavy metals. Cd and Cu were mostly found in cement plant and mine, respectively, and Cr, Ni, Pb and Zn were significant higher in main road, compared with other environmental conditions. The average values of EF for all the metals expect Cr in scenic and village area were over 1. The average values of EF for all the metals in mine, power plant, main road and cement plant were above 3. The overall pollution condition of heavy metals in Huainan City followed the order of Cd > Cu > Zn > Ni > Pb > Cr.

Published
2014

28. [Leaf micro-morphology and features in adsorbing air suspended particulate matter and accumulating heavy metals in seven trees species].

Author

Liu L, Fang YM, Wang SC, Xie Y, and Yang DD

Subjects
Adsorption, China, Cinnamomum camphora metabolism, Ligustrum metabolism, Plant Leaves ultrastructure, Trees metabolism, Air Pollution prevention & control, Ginkgo biloba metabolism, Metals, Heavy metabolism, Particulate Matter metabolism, Plant Leaves metabolism
Abstract

The purpose of this study was to assess the relationship between tree leaf micro-morphology and features in adsorbing air suspended particulate matter and accumulating heavy metals. Seven tree species, including Ginkgo biloba, at heavy traffic density site in Huainan were selected to analyze the frequency of air particulate matter retained by leaves, the particle amount of different sizes per unit leaf area retained by leaves and its related micro-morphology structure, and the relationship between particle amount of different sizes per unit leaf area retained by leaves and its related accumulation of heavy metals. We found that the species characterized by small leaf area, special epidemis with abundant fax, and highly uneven cell wall, as well as big and dense stomata and without trichomes mainly absorbed fine particulate matter; while those species with many trichomes mainly retained coarse particulate matter. Accumulation of heavy metals in leaves of the seven species was significantly different except for Ph. Tree species with high capacities in heavy metal accumulation were Ginkgo biloba, Ligustrum lucidum, and Cinnamomum camphora. Accumulation of Cd, Cr, Ni, Zn, Cu and total heavy metal concentration for seven tree species was positively related to the amount of particulate matter absorbed. Correlation coefficients of d10 vs d2.5, d10 vs d1.0, d2.5 vs d1.0 were 0.987, 0.971, 0.996, respective, and the correlate level was significant. The ratios of d2.5/d10, d1.0/d10, d1.0/d2.5 were 0.844, 0.763, 0.822, indicating that the particulate matter from traffic was mainly fine particulates.

Published
2013

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