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1. Research on the Critical Concerns of Software V&V for NPP I&C System Important to Safety

Author

Wang, Sheng-Chao, Du, Xin, Li, Heng, Xiao, Zhou, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Li, Yong, Series Editor, Liang, Qilian, Series Editor, Martín, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zamboni, Walter, Series Editor, Zhang, Junjie James, Series Editor, Xu, Yang, editor, Sun, Yongbin, editor, Liu, Yanyang, editor, Gao, Feng, editor, Gu, Pengfei, editor, and Liu, Zheming, editor

Published
2022
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3. Effect of fermentation on the constituents in the branches and leaves of Taxus media and non-small cell lung cancer.

Author

Guo, Xing, Wang, Rui-Sheng, Zhang, Zhen-Ling, Zhang, Hong-Wei, Wang, Sheng-Chao, Zhang, Shuai, Wu, Ya-Ning, Li, Ya-Jing, and Yuan, Jun

Subjects
NON-small-cell lung carcinoma, MTOR protein, ORGANIC acids, HIGH performance liquid chromatography, ENZYME-linked immunosorbent assay
Abstract

Introduction: Non-small cell lung cancer (NSCLC) is a prominent lung cancer disease worldwide. Currently, commonly used methods, such as surgery and radiotherapy, have significant side effects. Traditional Chinese medicine (TCM) has become a research hotspot because of its safe and effective characteristics. The branches and leaves of Taxus media are abundant in antitumor active compounds, and there has been no research conducted as yet regarding its anti–lung cancer molecular mechanism. Objective: The aim of this study is to investigate the antitumor activity of two samples before and after fermentation of T. media , and to research the molecular mechanism of its inhibitory effect on NSCLC. Methods: The chemical composition of pre-fermentation T. media (TM) and post-fermentation T. media qu (TMQ) were investigated using UHPLC-Q-Qrbitrap HRMS and high-performance liquid chromatography (HPLC). The anti-lung cancer activities of TM and TMQ were compared using an A549-induced tumor mouse model. An enzyme-linked immunosorbent assay (ELISA), hematoxylin and eosin (H&E) staining, immunohistochemistry, and immunofluorescence were used to determine the of TMQ mechanism of action. Results: The results indicated that TM and TMQ contained 83 compounds, consisting primarily of flavonoids, organic acids, and taxanes. Both taxanes and flavonoids in TMQ were higher than that in TM. Both TM and TMQ effectively inhibited the tumor growth in non-small cell lung cancer (NSCLC), and the inhibition rate was greater in TMQ (57.24%) than in TM (49.62%). TMQ administration downregulated the tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and the glutathione (GSH) level and upregulated interferon-γ (IFN-γ), reactive oxygen species (ROS), and malondialdehyde (MDA) levels in the serum of tumor mice. TMQ treatment also increased the protein expression of Bax, Caspase-3, and Beclin-1 in tumor tissues. In contrast, the bcl-2, PI3K, Ki67, ULK1, and mTOR protein levels were suppressed by TMQ. Protein assay analyses reemphasized the superior antitumor effect of TMQ over TM. These cumulative findings demonstrated that the mechanism of action of TMQ was closely related to the activation of transcriptional misregulation in the cancer pathway that inhibited the cholinergic synaptic, AMPK, and PI3K/Akt/mTOR signaling pathways. Conclusion: This study demonstrated that fermentation increased the active ingredient contents and antitumor effects of T. media. In addition, post-fermentation TMQ was superior to TM as a herbal medicine for NSCLC treatment. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Research and Application of the Verification and Validation Method Based on Embedded Technology in Nuclear Power Plants

Author

Zhang, Chao, Ye, Wang-Ping, Wang, Sheng-Chao, Shi, Ji, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Li, Yong, Series Editor, Liang, Qilian, Series Editor, Martín, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zamboni, Walter, Series Editor, Zhang, Junjie James, Series Editor, Xu, Yang, editor, Sun, Yongbin, editor, Liu, Yanyang, editor, Gao, Feng, editor, Gu, Pengfei, editor, and Liu, Zheming, editor

Published
2021
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5. Research and Application of FPGA V&V Technology in NPP Safety I&C System

Author

Wang, Sheng-Chao, Ye, Wang-Ping, Bai, Tao, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Li, Yong, Series Editor, Liang, Qilian, Series Editor, Martín, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zamboni, Walter, Series Editor, Zhang, Junjie James, Series Editor, Xu, Yang, editor, Sun, Yongbin, editor, Liu, Yanyang, editor, Gao, Feng, editor, Gu, Pengfei, editor, and Liu, Zheming, editor

Published
2021
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6. Research of Software V&V Technology in the Non-safety DCS of NPPs

Author

Wang, Sheng-Chao, Ye, Wang-Ping, Tang, Jian-Zhong, Bai, Tao, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Li, Yong, Series Editor, Liang, Qilian, Series Editor, Martín, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zamboni, Walter, Series Editor, Zhang, Junjie James, Series Editor, Xu, Yang, editor, Sun, Yongbin, editor, Liu, Yanyang, editor, Gao, Feng, editor, Gu, Pengfei, editor, and Liu, Zheming, editor

Published
2021
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7. Research and Application of Software Reliability Analysis Method for Safety I&C System in NPPs

Author

Wang, Sheng-Chao, Tang, Jian-Zhong, Bai, Tao, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Liang, Qilian, Series Editor, Martin, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zhang, Junjie James, Series Editor, Xu, Yang, editor, Sun, Yongbin, editor, Liu, Yanyang, editor, Wang, Yanjun, editor, Gu, Pengfei, editor, and Liu, Zheming, editor

Published
2020
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9. A Study About Software V&V Evaluation of Safety I&C System in Nuclear Power Plant

Author

Gu, Peng-Fei, Liu, Zhe-Ming, Xiong, Wei, Chen, Wei-Hua, Wang, Sheng-Chao, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Liang, Qilian, Series Editor, Martin, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zhang, Junjie James, Series Editor, Xu, Yang, editor, Xia, Hong, editor, Gao, Feng, editor, Chen, Weihua, editor, Liu, Zheming, editor, and Gu, Pengfei, editor

Published
2019
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10. A Study About Pre-developed Software Qualification of Smart Devices Applied in NPP

Author

Wang, Sheng-Chao, Bai, Tao, Gu, Peng-Fei, Ye, Wang-Ping, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Liang, Qilian, Series Editor, Martin, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zhang, Junjie James, Series Editor, Xu, Yang, editor, Xia, Hong, editor, Gao, Feng, editor, Chen, Weihua, editor, Liu, Zheming, editor, and Gu, Pengfei, editor

Published
2019
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11. Software Requirement Evaluation Method for Safety I&C System of Nuclear Power Plant

Author

Tang, Jian-Zhong, Gu, Peng-Fei, Wang, Sheng-Chao, He, Ya-Nan, Chen, Wei-Hua, Angrisani, Leopoldo, Series editor, Arteaga, Marco, Series editor, Chakraborty, Samarjit, Series editor, Chen, Jiming, Series editor, Chen, Tan Kay, Series editor, Dillmann, Ruediger, Series editor, Duan, Haibin, Series editor, Ferrari, Gianluigi, Series editor, Ferre, Manuel, Series editor, Hirche, Sandra, Series editor, Jabbari, Faryar, Series editor, Kacprzyk, Janusz, Series editor, Khamis, Alaa, Series editor, Kroeger, Torsten, Series editor, Ming, Tan Cher, Series editor, Minker, Wolfgang, Series editor, Misra, Pradeep, Series editor, Möller, Sebastian, Series editor, Mukhopadhyay, Subhas Chandra, Series editor, Ning, Cun-Zheng, Series editor, Nishida, Toyoaki, Series editor, Panigrahi, Bijaya Ketan, Series editor, Pascucci, Federica, Series editor, Samad, Tariq, Series editor, Seng, Gan Woon, Series editor, Veiga, Germano, Series editor, Wu, Haitao, Series editor, Zhang, Junjie James, Series editor, Xu, Yang, editor, Gao, Feng, editor, Chen, Weihua, editor, Liu, Zheming, editor, and Gu, Pengfei, editor

Published
2018
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20. Distinct roles of EGF repeats for the Notch signaling system

Author

Wang Sheng Chao, Kei Sakamoto, Ken-ichi Katsube, and Akira Yamaguchi

Subjects
EGF-like domain, Amino Acid Motifs, Notch signaling pathway, Chick Embryo, Biology, Cell fate determination, Ligands, Cell Line, Mice, Epidermal growth factor, Chlorocebus aethiops, Animals, Drosophila Proteins, Humans, HES1, Promoter Regions, Genetic, Repetitive Sequences, Nucleic Acid, Epidermal Growth Factor, Receptors, Notch, Cell Membrane, Membrane Proteins, Cell Biology, Transmembrane protein, Cell biology, Biochemistry, Notch proteins, COS Cells, NIH 3T3 Cells, Drosophila, Signal transduction, Dimerization, Signal Transduction
Abstract

Notch is a single-pass transmembrane receptor that mediates cell fate choice in various species and developmental contexts. The Notch signal is transduced by its intracellular domain, which acts as a transcriptional activator, and is released from the plasma membrane by proteolytic cleavages. This process is initiated by intercellular association of the epidermal growth factor (EGF) repeats between Notch and the DSL (Delta, Serrate, Lag-2) ligands but the detailed mechanism is yet to be clarified. Here we demonstrate that Notch1 can form homodimers, which is achieved by its EGF motifs. The Notch1 dimer formation increased in response to ligand presentation and HES1 promoter was stimulated, implying that receptor homodimerization is an important initial step in Notch signal transduction. EGF motifs also serve as a protection against proteases, including TNF-alpha converting enzyme, which prevents Notch1 from ligand-independent activation. Multiple functions of the Notch EGF motifs, such as the prevention of constitutive activation, reciprocal interaction with the ligands and lateral interaction for homodimerization, appear to constitute crucial elements of the Notch signaling system.

Published
2004

25. [DuzhongButiansu Capsules improve adenine-induced reproductive dysfunction in male rats].

Author

Wang SC, Li BK, Kan YX, Zeng MN, Zhang BB, Liu L, Zheng XK, and Feng WS

Subjects
Adenine, Animals, Capsules, Epididymis, Female, Male, Oxidative Stress, Pregnancy, Random Allocation, Rats, Rats, Sprague-Dawley, Sexual Dysfunction, Physiological chemically induced, Spermatozoa, Testis, Drugs, Chinese Herbal therapeutic use, Sexual Dysfunction, Physiological drug therapy, Sperm Motility
Abstract

Objective: To study the effect of DuzhongButiansu Capsules (DBC) on adenine-induced reproductive dysfunction (RD) in male rats., Methods: Eighty male SD rats were randomly divided into six groups, blank control (n = 8), solvent control (n = 8), RD model control (n = 16), Shengjing Capsules (SJC) (n = 16), low-dose DBC (n = 16) and high-dose DBC (n = 16). The RD model was made by intragastric administration of adenine at 200 mg/kg/d for 5 successive weeks in the latter four groups of animals, and in the meantime the rats in the latter three groups were treated intragastrically with SJC at 0.560 mg/kg/d and DBC at 0.242 and 0.968 mg/kg/d, respectively. At the end of the fourth week, all the rats were mated with female ones in a 1:1 ratio for 7 days. Then the male rats were killed and the right epididymides collected for detection of sperm concentration and motility, and the female ones sacrificed after fed for another 2 weeks and the numbers of pregnancies and fetal rats were recorded. The heart, liver, spleen, lung, kidney, thymus, testis, epididymis and seminal vesicle were harvested for obtainment of the visceral coefficients and semen parameters, observation of the histopathological changes in the testis, epididymis and kidneys by HE staining, measurement of the levels of serum T, E2, FSH and LH by ELISA, detection of the contents of serum glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), malondialdehyde (MDA) and creatinine (Scr), blood urea nitrogen (BUN), and determination of the expressions of Bax, Bcl-2, Caspase-3 and Caspase-9 proteins in the renal tissue by immunohistochemistry., Results: No statistically significant difference was observed between the blank control and solvent control groups in any of the indexes obtained (P > 0.05).Compared with the blank controls, the rats in the RD model control group showed significantly decreased sperm concentration (［40.67 ± 7.37］vs ［27.10 ± 2.72］ ×10⁶/ml, P < 0.01), sperm motility (［54.75 ± 3.92］%vs ［25.60 ± 4.83］%, P < 0.01) and pregnancy rate (85.7% vs 43.8%, P < 0.01). The rats in thelow- and high-dose DBCgroups exhibited remarkable increases in sperm concentration (［53.00 ± 4.55］% and ［65.63 ± 12.47］% ×10⁶/ml, P < 0.01) and sperm motility (［53.50 ± 8.83］% and ［54.33 ± 7.92］ %, P < 0.01), and so did those in the high-dose DBC group in pregnancy rate (54.5%, P < 0.01).After medication, the animals showed markedly increased body weight and visceral coefficients of the testis, epididymis and seminal vesicle (P < 0.05 or P < 0.01), recovered morphology of the testis, epididymis and kidneys, reduced levels of Scr, BUN, FSH, LH and MDA in the serum (P < 0.05 or P < 0.01), increased contents of T, SOD and GSH-PX (P < 0.05 or P < 0.01), down-regulated expressions of Bax, Caspase-3 and Caspase-9 and up-regulated expression of Bcl-2 in the renal tissue (P < 0.05 or P < 0.01)., Conclusions: DBC can improve adenine-induced reproductive dysfunction in male rats, which may be attributed to its effects of inhibiting the apoptosis of proteins, improving oxidative stress and elevating the levels of reproductive hormones.

Published
2020

26. Status of Tooth Loss and Denture Restoration in Chinese Adult Population: Findings from the 4th National Oral Health Survey.

Author

Guo J, Ban JH, Li G, Wang X, Feng XP, Tai BJ, Hu Y, Lin HC, Wang B, Si Y, Wang CX, Rong WS, Wang WJ, Zheng SG, Liu XN, and Wang SC

Subjects
Adult, Aged, China epidemiology, Dental Health Surveys, Dentures statistics & numerical data, Female, Humans, Male, Middle Aged, Mouth, Edentulous rehabilitation, Rural Population statistics & numerical data, Sex Factors, Tooth Loss rehabilitation, Urban Population statistics & numerical data, Denture, Partial, Fixed statistics & numerical data, Denture, Partial, Removable statistics & numerical data, Mouth, Edentulous epidemiology, Tooth Loss epidemiology
Abstract

Objective: To investigate the status of tooth loss and denture restoration in Chinese adults, analyse the changing trend and provide fundamental data for oral health policy., Methods: According to the protocol of the 4th National Oral Health Survey, a multistage stratified random cluster-sampling method was used to enrol adult subjects aged 35 to 44, 55 to 64 and 65 to 74 years in all 31 provinces, municipalities and autonomous regions of the mainland of China. The status of tooth loss and denture restoration was investigated. SPSS20.0 software was used for statistics analysis., Results: Among the 13,464 subjects investigated, 13.8% had complete dentition, 84.4% had dentition defects, and 1.8% was edentulous. Urban subjects showed a significantly higher proportion of complete dentition than those in rural (P = 0.02), and males showed the statistically higher proportion of complete dentition than females (P = 0.01). The mean of remaining teeth was 26.1 ± 6.90, which in urban areas was significantly higher than in rural areas (P < 0.01). The means of remaining teeth were 29.6 ± 2.3, 26.3 ± 6.1, and 22.5 ± 8.7 in the 35 to 44, 55 to 64 and 65 to 74 age groups, respectively. The detection rate of fixed partial dentures (FPD) was statistically higher in urban than in rural areas and in males than that in females (P < 0.01). The detection rate of removable partial dentures (RPD) was statistically higher in urban areas than in rural locations (P < 0.01). However, the detection rates of irregular denture and unrepair of tooth loss were both significantly lower in urban than in rural areas (P < 0.01). The rate of restoration of tooth loss was 41.6% in Chinese adults., Conclusion: Although the tooth loss and denture restoration status recorded in the survey was improved compared with the results of 10 years ago, more efforts need to be made on strengthening oral health promotion, particularly for elderly people and those living in rural areas.

Published
2018
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27. [Construction, expression and identification of recombinant plasmid encoding bifunctional protein sflk1-IFN-gamma].

Author

Wu QQ, Chen HH, Guo J, Wang SC, and Pan JP

Subjects
Animals, COS Cells, Chlorocebus aethiops, Female, Interferon-gamma genetics, Mice, Mice, Inbred C57BL, Plasmids, Recombinant Proteins analysis, Recombinant Proteins genetics, Reverse Transcriptase Polymerase Chain Reaction, Transfection, Vascular Endothelial Growth Factor Receptor-2 genetics, Interferon-gamma biosynthesis, Recombinant Proteins biosynthesis, Vascular Endothelial Growth Factor Receptor-2 biosynthesis
Abstract

Objective: To construct, express and identify the recombinant plasmid pcDNA3.1(+)/sflk1-IFN-gamma encoding bifunctional protein sflk1-IFN-gamma (soluble fetal liver kinase 1 and interferon-gamma)., Methods: sflk1 and IFN-gamma gene fragments were cloned by RT-PCR, and then inserted into pcDNA3.1(+) plasmid between BamHI-EcoRI and XhoI-XbaI restriction sites to form the recombinant plasmid pcDNA3.1(+)/sflk1-IFN-gamma. The recombinant sflk1-IFN-gamma transiently expressed in COS-7 cells was detected by ELISA and Western blotting. Bioactivities of sflk1-IFN-gamma fusion protein were identified by proliferation inhibition assay with H5V cells and NK activity assay., Results: pcDNA3.1(+)/sflk1-IFN-gamma can be effectively expressed in COS-7 cells. Concentrations of sflk1 and IFN-gamma in culture supernatants of pcDNA3.1(+)/sflk1-IFN-gamma transfected COS-7 cells were (20.85+/-2.48) ng/ml and (1.08+/-0.09) ng/ml, respectively. Western blotting showed that the molecular weight of sflk1-IFN-gamma fusion protein was about 130 kDa, while that of sflk1 was 115 kDa. The supernatants of transfected cells significantly inhibited the proliferation of H5V cells stimulated by mouse VEGF 164 and enhanced the NK activity of splenocytes, demonstrating that sflk1-IFN-gamma fusion protein possessed the bioactivities of both sflk1 and IFN-gamma., Conclusion: The constructed plasmid pcDNA3.1(+)/sflk1-IFN-gamma can be effectively expressed in eukaryotes. The expressed sflk1-IFN-gamma fusion protein has the biological activities of both sflk1 and IFN-gamma.

Published
2010
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