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Objective: This study aimed to optimize the process conditions for the large-scale production of milk-derived casein glycomacropeptide （CGMP） and explore its hemagglutination inhibition （HI） ability against influenza virus. Methods: Using anion exchange resin as the separation medium and sialic acid content the activity detection index of the product,based on the preliminary small and medium-sized tests, the large-scale production of CGMP from whey powder was investigated from five process conditions: sample volume, loading flow, eluent concentration, eluent volume, and resin service cycle. Meanwhile, a hemagglutination inhibition test was used to verify the inhibitory activity of the product CGMP against the influenza virus. Results: The optimized technical route of CGMP product is as follows: loading volume 3BV, loading flow 0.8 L/min, elution volume 1BV, eluent concentration 0.6 mol/L, and suitable use cycle of resin 4 times. Under these conditions, the volume of whey powder solution was 108 L （2 160 g） and the resin was 36 L, and 21.183 g of CGMP product was obtained, with a yield of 0.981 g/100 g, the product purity of 89.032%, the recovery rate of 51.972% and the sialic acid content of 21.503 g/100 g. The minimum inhibitory concentration of CGMP products on influenza virus A （H1N1） and influenza virus B is 1.000 0 and 1.250 0 mg/mL, respectively. Conclusion: The purity and sialic acid content of CGMP produced by the optimized technical route were better than those of commercial CGMP, and had an obvious inhibitory effect on the influenza virus.

The intestinal flora of the human body is closely related to health. Gut microbiota dysbiosis will lead to intestinal dysfunction and, consequently, a variety of intestinal diseases. In this study, subjects with different diseases were requested to take one of three microecological agents, stachyose, composite probiotics (CPb) and synbiotic MF-13. An Ion Torrent PGM™ sequencing platform was used to analyze the structure of intestinal microflora and the contents of cholesterol and bile acid in the feces of the subjects were measured. Our aim was to investigate the regulatory effect of intervention with microecological agents on the intestinal microflora structure and lipid metabolism. The results showed that 1 067 operational taxonomic units (OUT) were obtained from the fecal microbial samples from all subjects. The dominant bacteria were Firmicutes and Bacteroidetes, altogether accounting for 91.65%, and Bacteroidetes accounting for about 70.10% of the total number of sequences. Bacteroides and Prevotella were the dominant genera, accounting for 33.1% and 33.8% of the total sequences, respectively. At the phylum level, stachyose promoted the proliferation of Bacteroidetes, but inhibited the proliferation of Firmicutes. CPb had no significant effect on the abundance of each phylum, while MF-13 promoted the proliferation of Proteobacteria and Actinobacteria. Stachyose significantly decreased the abundance of Sutterella in subjects with constipation and insomnia, CPb significantly decreased the abundance of Sutterella in subjects with diarrhea, and MF-13 increased the abundance of Parabacteroides. The analysis of intestinal microflora diversity showed that Chao1 index significantly increased during the early period of MF-13 intervention, while there were no significant changes in other diversity indexes. Cluster analysis and non-metric multi-dimensional scaling (NMDS) analysis showed that microecological preparation intervention changed the intestinal microbiota structure in most of the samples, and the microflora structure of all populations showed an overlapping tendency. The intestinal microflora species that significantly differed between individuals were analyzed using Metastat statistics, and it was found that there was no significant change in the abundance of OTUs before and after intervention with each microecological agent, while there was a significant difference between individual subjects. MF-13 significantly promoted the fecal excretion of cholesterol and bile acids (P < 0.05). In conclusion, stachyose has an obvious influence on the intestinal microflora structure of volunteers, while CPb and MF-13 do not. However, MF-13 can increase the intestinal excretion of cholesterol and bile acid, which is beneficial to the metabolism of intestinal cholesterol, but the durability of this effect is poor.

Objective To explore the effect of diet coke combined with chlorhexidine gargle in oral care of tracheal intubation patients in Neurology ICU.Methods From October 2019 to October 2022, 110 patients with tracheal intubation who were admitted to the Neurology Intensive Care Unit of Huai'an First People's Hospital were selected as the research object. The patients were randomly divided into control group and observation group (n=55, each). The control group received routine oral care (wiping with saline cotton balls). In the observation group, the diet coke combined with chlorhexidine gargle was used for negative pressure washing. The incidence of ventilator-associated pneumonia (VAP), improved Beck oral score, mechanical ventilation time, and ICU hospitalization time between two groups were compared after implementing oral care. Results After implementing oral care, the incidence of VAP in the intervention group was lower than that in the control group [9.09%（5/55） vs 32.73%(18/55), χ=9.29, P＜0.05]. Compared with the control group, the observation group had lower Beck oral score (P＜0.05), ICU hospitalization time [(7.71±0.66) d vs (12.00±0.72) d, P＜0.05], and mechanical ventilation time[(5.82±0.77) d vs (8.36±0.73) d, P＜0.05]. Conclusion Diet coke combined with chlorhexidine mouthwash under negative pressure can improve oral hygiene, reduce the incidence of VAP, and shorten the hospital stay and mechanical ventilation time in ICU.

Myofascial as a holistic structure emphasizes a holistic approach to intervention and treatment of fascial-related disorders such as neck pain (NP), low back pain (LBP), and knee pain. There are currently adverse effects of medication for diseases related to myofascial. Traditional Chinese rehabilitation exercise (TCRE) is a practical approach to traditional Chinese medicine and is a valuable option for intervening in myofascial-related pain. This article found some research evidence for Baduanjin, Wuqinxi, and Yijinjing in clinical studies of myofascial chain-related pain. The article summarizes the current evidence and finds that TCRE can enhance limb movement function through breathing and slow movements, increase joint movement and flexibility, and reduce joint pathology and stress-induced pain. As for future directions, focus on TCRE in improving the health of older adults and treating long-COVID syndrome, and integrate robotic and TCRE training to frame safe and effective exercise models. Relevant studies have already been registered in the Clinical Trials Registry, and some clinical study protocols have been published. TCRE can be an alternative nonpharmacological rehabilitation therapy to alleviate chronic rheumatic pain symptoms and augment public health management. [ABSTRACT FROM AUTHOR]