16 results on '"Wang, Ming-Jin"'
Search Results
2. Enfortumab-vedotin use for urothelial carcinoma in two patients on hemodialysis
- Author
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Khosla, Harshit, primary, Bhatt, Sita, additional, Wang, Ming-Jin, additional, Gignac, Gretchen, additional, Mittal, Kriti, additional, and Patel, Jasmine, additional
- Published
- 2024
- Full Text
- View/download PDF
3. Chlorogenic Acid Induced Neuroblastoma Cells Differentiation via the ACAT1-TPK1-PDH Pathway.
- Author
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You, Shen, Wang, Ming-Jin, Hou, Zhen-Yan, Wang, Wei-Da, Du, Ting-Ting, Xue, Ni-Na, Ji, Ming, and Chen, Xiao-Guang
- Subjects
- *
CELL differentiation , *CHLOROGENIC acid , *NEUROBLASTOMA , *ANTINEOPLASTIC agents , *VITAMIN B1 , *LABORATORY mice - Abstract
Background: Chlorogenic acid (CHA) has been shown to have substantial biological activities, including anti-inflammatory, antioxidant, and antitumor effects. However, the pharmacological role of CHA in neuroblastoma has not yet been assessed. Neuroblastoma is a type of cancer that develops in undifferentiated sympathetic ganglion cells. This study aims to assess the antitumor activity of CHA against neuroblastoma and reveal its mechanism of action in cell differentiation. Methods: Be(2)-M17 and SH-SY5Y neuroblastoma cells were used to confirm the differentiation phenotype. Subcutaneous and orthotopic xenograft mouse models were also used to evaluate the antitumor activity of CHA. Seahorse assays and metabolomic analyses were further performed to investigate the roles of CHA and its target ACAT1 in mitochondrial metabolism. Results: CHA induced the differentiation of Be(2)-M17 and SH-SY5Y neuroblastoma cells in vivo and in vitro. The knockdown of mitochondrial ACAT1, which was inhibited by CHA, also resulted in differentiation characteristics in vivo and in vitro. A metabolomic analysis revealed that thiamine metabolism was involved in the differentiation of neuroblastoma cells. Conclusions: These results provide evidence that CHA shows good antitumor activity against neuroblastoma via the induction of differentiation, by which the ACAT1-TPK1-PDH pathway is involved. CHA is a potential drug candidate for neuroblastoma therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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4. Yttrium-90 Hepatic Therapy and the Increasing Role of Volumetric Voxel-based Post Therapy Dosimetry: A Case Report
- Author
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Ding, Linda, primary, Sioshansi, Shirin, additional, Malik, Hesham, additional, Franquet-Elia, Elisa, additional, McIntosh, Lacey, additional, Ruppell, Evan, additional, Licho, Robert, additional, Kim, Young, additional, Goldstein, Alan, additional, Mittal, Kriti, additional, Wang, Ming-Jin, additional, Gultawatvichai, Patan, additional, Mehta, Savant, additional, Foley, Kimberly, additional, Wilson, Sean, additional, Bishop-Jodoin, Wilson, additional, and FitzGerald, Thomas, additional
- Published
- 2022
- Full Text
- View/download PDF
5. Contributors
- Author
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Abelman, Rebecca A., Abrams-Downey, Alexandra, Adams, Emily, Afari, Maxwell Eyram, Akoto, Shanice, Al Jandali, MHD Hussam, Alhudairy, Maad M., Ali, Tanya, Alipanah-Lechner, Narges, Allen, Stephanie M., Alomari, Ihab, Alpert-Rathhauser, Erin, Ander, Ilana, Andrievskaya, Maria, Anilowski, Kathryn Taylor, Anthony, AnnGene, Aoun, Joe, Arzomand, Zuhal, Ashtiani, Ali, Asiedu, Daniel K., Astsaturov, Artem, Aulakh, Sudeep K., Avasare, Rupali, Aziz, Sarah, Babar, Tania B., Bachman, Emelia Argyropoulos, Bains, Ashika, Bank, T. Caroline, Banwell, Brenda, Barrett, Trace, Barseghian, Ailin, Basman, Craig L., Bawaadam, Hasnain, Beckwith, Noor, Belcher, Ren, Bell, Jennifer, Bellinghausen, Amy L., Benner, Deanna, Benson, Nicole M., Bergen, Michael, Berger, Agatha, Berges, Arnaldo A., Berkowitz, Carol, Bevington, Kristin, Bhagat, Vicky H., Bhatt, Harikrashna B., Bhuptani, Prachi H., Bilodeau, Courtney Clark, Bissonette, Stefani, Bitar, Ghamar, Blakeney, Craig, Blankenhorn, Brad, Block, Brian, Blomberg, Christopher P., Boduch, Abigail N., Bokshan, Steven L., Bortz, Christina M., Boulos, Alexandre, Bouton, Tara C., Bove, Mary E., Bowlby, Lynn A., Box, Amanda, Brady, Mark F., Bratman, Russell E., Brennan, Keith, Bridges, Amy, Brown, Emily, Budzyn, Jeffrey, Bui, Eric, Burke, Christine, Byanova, Katerina L., Cahill, Kate, Canty, Katherine, Cao, Damon, Caraganis, Andrew, Carpenter, Randy S., Carragee, Eugene, Carter, Ashlie Sewdass, Castaneda-Guarderas, Ana, Castillo, Jorge J., Catana, Andreea M., Cerezo, Carolina S., Chan, Philip A., Chang, Lucy Y., Chapman, Arlene, Chawla, Anjulika, Chemchirian, Hrak, Cheng, Vicky, Cherian, Tracy, Chin, Robin, Chis, Roxana, Chitturi, Chandrika, Chofay, Dana Ricci, Cholankeril, George, Cholankeril, Rosann, Chou, Stella T., Choudhry, Aruj, Choxi, Shivali, Christian, Cindy W., Chua, Winona, Clark, Seth, Clebak, Karl T., Cohen, Debbie L., Cohen, Lisa, Cohen, Phillip D., Cohn, Zachary, Congdon, Morgan, Constantinou, Maria, Copelin, Eddie L., II, Corley, James Earl, III, Craine, Rebecca, Cristofaro, Patricia, Crouss, Tess, Cruz, Aristides I., Cunha, Joanne Szczygiel, Cunningham, Karlene, Currier, Chase, Curtin, Alicia J., Cusano, Joseph, Dabiri, Ganary, Dado, Lynn, Dalal, Deepan S., Daly, Gerhard H., Danak, Shivang U., Das, Rituparna, DaSilva, Manuel F., Dave, Natasha, Davis, Mackenzie, Dedent, Alison, Denninger, John, Desrochers, Colette, Detweiler, Jacquelyn, Devon, Erin Pete, Dhokte, Ashwini U., Diaz, Joseph A., DiSilvestro, Kevin J., Dohlman, Thomas H., Dolter, Stephen, Domenichini, David J., Doo, Kathleen, Dove, James H., Duh-Leong, Carol, Duker, Andrew P., Dwivedi, Shashank, Dworkin, Myles, Eisenhower, Christine, Elghafri, Amani A., Ellsworth, Pamela, Epstein, Alan, Espinosa, Patricio Sebastian, Evans, Andrew R., Everly, Eileen, Faber, Mark D., Fabio, Mary, Facer, Evan, Fadale, Paul, Fagan, Matthew J., Farkas, Jon, Farrand, Erica, Fay, Kevin, Fernandez-Robles, Carlos, Ferreira, Jason D., Ferri, Fred F., Ferri, Heather, Fischer, Staci A., Fonge, Yaneve, Forbes, Kellie C., Forcier, Michelle, Ford, Sydney, Fort, Frank G., Fort, Glenn G., Fraser, Justin F., Fricchione, Gregory L., Friedman, Michael, Frisch, Daniel R., Fusunyan, Mark, Gallo, Anthony, Garg, Rimmy, Gasga, Arturo, Gentile, Tatiana Londoño, Gesthalter, Yaron B., Ghanim, Mostafa, Ghazarian, Armen S., Gidwani, Nisha H., Gil, Joseph, Gitelmaker, Dimitri, Godding, Mark, Goldburt, Alla, Goldfarb, Danielle, Goldsmith, Corey, Gangadhara Rao Golla, Maheswara Satya, Gomez, Helen B., Goodman, Avi D., Gordon, Paul, Gray, John A., Greenfield, Morgan, Grisham, Lindsey Anne, Gultawatvichai, Patan, Guo, David, Gupta, Priya Sarin, Guttadauria, Brittany, Guzman, Herodes, Hack, Nawaz K.A., Handa, Sajeev, Harbord, Nikolas, Hardy, Erica, Harrington, Colin J., Hartvigsen, Nathan, Havele, Sonia A., Hayes, Don, Jr., Hehar, Jaspreet K., Heinle, Rachel Wright, Helm, Leesha A., Helm, Matthew F., Hennebery, Ruth B., Herek, Jyothsna I., Hewson, Melissa A., Hines, Margaret R., Hladky, Katherine, Hodges, Ashley, Hoffman, R. Scott, Hogan, Dawn, Holiman, Jennifer J., Holland, N. Wilson, Holmes, Karima A., Holton, Siri M., Hoy, Austin, Hsu, Raymond, Huang, Elena, Huang, Lawrence, Hume, Anne L., Hussain, Zilla, Huynh, Donny V., Hyder, Sarah, Ibrahim, Dina A., Ibrahim, Zainab, Ingraham, Caitlin, Inman, Nicholas J., Insalaco, Louis F., Ivkovic, Ana, Jacobson, Ashley A., Jafri, S. Hammad, Jain, Koyal, Jain, Vanita B.D., Jamal, Fariha, Jamil, Marcus L., Janigian, Robert H., Jr., Jasuja, Sonia, Javier, Noelle Marie, Jenkins, Derek R., Jetty, Vybhav, Jin, Lillian, Johnson, Katie, Johnson, Michael P., Jolly, Angad, Jonas, Rebecca, Jones, Matthew C., Joshi, Priyanka, Joshi, Shyam, Justice, Joshua, Karthikeyan, Vanji, Kass, Joseph S., Kass, Naomi R., Kassam, Zain, Katarincic, Julia, Kaul, Bhavika, Kaur, Gurmehr, Kazim, Ali, Kazzaz, Sudad, Kedar, Sachin, Keleshian, Vasken, Keselman, Dennis, Khan, Rizwan, Khare, Sarthak, Khawaja, Hussain R., Kirchner, Robert M., Kohrman, Samuel I., Kojic, Erna Milunka, Kokkirala, Aravind Rao, Konstantino, Yuval, Kopyt, Nelson, Kosinski, Lindsay R., Kothadia, Savan, Koulouridis, Ioannis, Kovacevic, Natalija, Kreider, Timothy R., Krishnamohan, Prashanth, Krueger, Van S., Kumbar, Lalathaksha, Kuris, Eren O., Kurss, David I., Kutschke, Michael, LaCasce, Ann S., Lam, Uyen T., Lapite, Ajibike, Lau, Julianna, Leard, Lorriana E., Leavitt, David A., Lee, Hyun, Lee, Kachiu C., Lee, Yvonne, Lemme, Nicholas J., Lento, René, Levins, James G., Li, Jian, Li, Suqing, Lieberman, Leora, Lightner, Donita D., Lin, Erica, Longjohn, Cynthia, López, Gabriela, Lord, Kito Akin, Lowenhaupt, Elizabeth A., Lowery, Curtis (Trey) Lee, III, Lucier, David J., Jr., Maciag, Michelle C., MacLean, Rachel L., Madva, Elizabeth N., Maheswaran, Abiramy, Majmudar, Shefali, Makai, Gretchen, Malcolm, Katherine, Malhotra, Pieusha, Manjrekar, Eishita, Maracaccio, Stephen E., Masaki, Charles, Mazumdar, Maitreyi, Mazzaglia, Peter J., Mbuyi, Nadine, McCulloh, Russell J., McDonald, Christopher, McGowen, Alexandra, McGuirk, Barbara, McNamara, Kelly, Mercado, Jorge, Merrill, Scott J., Merriman, Jennifer B., Merritt, Rory, Metcalfe-Klaw, Robin, Michaels, Marian G., Michaud, Gaetane, Michel, Jeremy, Mikolasko, Brian D., Min, May, Minami, Taro, Minkel, Jared D., Mirza, Farhan A., Mistry, Hetal D., Mitsuyama, Rei, Modest, Jacob, Monachese, Marc, Monhollon, Ethan, Montgrain, Philippe, Mordehai, Eveline, Morelli, Leah, Mughal, Aleem I., Mujib, Marjan, Mukkamalla, Shiva Kumar R., Mullersman, Katherine M., Nadeem, Omar, Naseri, Hussain Mohammad H., Nasir, Uzma, Neithardt, Adrienne B., Ni, Samantha, Nisavic, Mladen, Nokoff, Natalie, Novak, James E., Nunneley, Chloe Mander, Nuss, Emily E., O’Donnell, Ryan M., Olszewski, Adam J., Onyeaka, Henry K., Orchowski, Lindsay M., Orman, Sebastian, Osborne, Ashley D., Owens, Brett D., Oyelakin, Ayotomide, Pace, Paolo G., Papaila, Alexa, Pappas-Taffer, Lisa, Pares, Marco, Parikh, Mihir, Parikh, Shivani, Partin, Michael T., Patel, Birju B., Patel, Devan D., Patel, Melissa D., Patel, Nima R., Patel, Pranav M., Patel, Shivani K., Patel, Shyam A., Patrick, Brett, Paudel, Shreedhar, Paul, Grace Rebecca, Paxton, E. Scott, Pearlstein, Ethan, Peck, Alexander, Perazella, Mark, Perez, David L., Pfeuti, Courtney, Pham, Lily C., Pham, Long, Phillips, Katharine A., Phillips, Oliver W., Phung, Quan, Plante, Wendy A., Plumley, Kevin V., Praschan, Nathan, Prashar, Rohini, Pressman, Amanda, Priestly, Margaret, Punko, Diana, Puthawala, Imran, Qawi, Imrana, Quan, Michele, Quijije, Nadia, Quinn, Matthew, Rachu, Gregory S., Raducha, Jeremy E., Rafeq, Samaan, Ranieri, Gina, Rathore, Bharti, Rathore, Ritesh, Raukar, Neha P., Reagan, John L., Reddy, Bharathi V., Reddy, Snigdha T., Reed, Griffin, Reginato, Anthony M., Reichart, James P., Reinhart, Michaela B., Restrepo, Judith A., Reus, Victor I., Revet, Alexis, Reyes, Candice, Rich, Harlan G., Richards, Rocco J., Richards, Sheyla, Riddell, Nathan, Righi, Giulia, Rivera, Alvaro M., Rivera, Lauren Davis, Riviere, Amy E., Roberts, Todd F., Robertson, Melena, Morfin Rodriguez, Alejandra E., Romero Gómez, Perla M., Roth, Julie L., Roussel, Breton, Rubeor, Amity, Rubin, David, Ruhstaller, Kelly, Saeed, Firrah, Safi, Javeryah, Saks, Emily, Sall, Jeffrey W., Samaniego-Picota, Milagros, Sanders, Wesley M., Santhosh, Lekshmi, Santos, Linda Herrera, Satiya, Jinendra, Satpathy, Hemant K., Satpathy, Ruby K., Sauck, Emily, Sayeed, Syeda M., Saylor, Leah, Scaramangas-Plumley, Daphne, Scheel, Paul J., Jr., Schlussel, Bradley, Schnitzer, Kristina, Sciscione, Anthony, Scully, Christina D., Seeber, Richard E., II, Gilek-Seibert, Katarzyna, Sell, Peter J., Sewell, Rachel, Shaban, Hesham, Shah, Ankur, Sharma, Animesh, Sharma, Esseim, Sharma, Gargi Priamvada, Sharma, Yuvraj, Sharp, Lydia, Sherman, Alexander, Shill, Jessica E., Shlossman, Philip A., Shrestha, Asha, Siddiqui, Khawja A., Siegel, Alyssa, Sigman, Mark, Singh, Harinder P., Singh, Vikas, Slate, Jerome L., Slingsby, Brett, Smick, Alexandra H., Smith, Jeanette G., Smith, Jonathan H., Smith, Matthew J., Soi, Vivek, Soinski, Rebecca, Soler, Maria E., Soman, Sandeep, Sorg, Emily, Spear, Timothy T., Sperati, C. John, Stern, Diana, Stern, Ella, Stockwell, Philip, Sudhakar, Padmaja, Suh, Edward, Sun, Jennifer K., Sutter, Stephanie, Sweeney, Joseph, Sweeney, Simone, Tabba, Maher, Takasaki, Kaoru, Takei, Ryosuke, Tammaro, Dominick, Tan, Laren, Taylor, Alan, Tellioglu, Tahir, Testa, Edward J., Thomas, Anthony G., Thome, Andrew P., Jr., Tien, Alexandra Meyer, Tien, David Robbins, Tierney, Anna-Marie, Tiffany, Patrick, Toma, Helen, Triplett, Thomas M., Trivedi, Hirsh D., Trivedi, Vrinda, Tryforos, Margaret, Tsaban, Gal, Tucci, Joseph R., Uduman, Junior, Uiterwyk, Sean H., Ukaegbu, Rebecca, Umanth, Kausik, Uy, Bryant, Vakili, Babak, Van Kirk, Emily, Vaughan, Jennifer E., Venado, Aida, Vijayakumar, Shilpa, Vollum, Kathryn G., Vorel, Ethan, Vu, Kimberly, Vutescu, Emil Stefan, Walker, J. Richard, III, Walsh, Ryan, Walson, Ifeanyi, Wang, Angela, Wang, Danielle, Wang, Evelyn R., Wang, Jarey H., Wang, Ming-Jin, Wang, Stephani C., Wang, Tisha, Watson, Ryan, Weekley, Manuel Shayne, Weinberg, Marc S., Weiss, Max, Walesko, Mary-Beth, Werth, Adrienne, White, Matthew J., Williamson, Patrick, Wincze, John P., Winikor, Jared M., Wininger, Bryce, Wolpert, Marlene Fishman, Wongsaengsak, Sariya, Wylie, John, Wu, Tzu-Ching (Teddy), Yang, Anna, Yang, Nicole B., Yee, Jerry, Yesodharan, Gemini, Yi, Jia, Yip, Agustin G., York, Robert J., Young, John Q., Young, Shane, Yusufani, Reem, Zahm, Caroline, Zdroik, Anna V., Zenlea, Talia, Zhou, Diana, Zia, B. Shoshana, Ziaie, Navid, Zimmerman, Mark, Zipursky, Rachel, Zollman, Joshua W., Zorin, Danylo, Zouk, Aline N., and Zurek, Olivia
- Published
- 2022
- Full Text
- View/download PDF
6. Primary Chest Wall Tumors
- Author
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Wang, Ming-Jin and Gultawatvichai, Patan
- Published
- 2022
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7. Catalytic Decomposition of N2O over M(10)/RPSA (M=Mg, Mn, Cu, Zn) Catalysts
- Author
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Wang, Ming Jin, primary, Wang, Hong, additional, Li, Cui Qing, additional, Ding, Fu Chen, additional, Song, Yong Ji, additional, and Cai, Ka Sha, additional
- Published
- 2014
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8. Th17 (IFNγ- IL17+) CD4+ T Cells Generated After Burn Injury May Be a Novel Cellular Mechanism for Postburn Immunosuppression
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Neely, Crystal J., primary, Maile, Robert, additional, Wang, Ming-Jin, additional, Vadlamudi, Sivaram, additional, Meyer, Anthony A., additional, and Cairns, Bruce A., additional
- Published
- 2011
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9. Severe burn injury leads to spontaneous generation of Th17 CD4+ T cells in wound draining lymph nodes (42.18)
- Author
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Neely, Crystal, primary, Maile, Rob, additional, Wang, Ming-jin, additional, Wolfgang, Matthew, additional, and Cairns, Bruce, additional
- Published
- 2010
- Full Text
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10. Burn injury induces a myeloid suppressor phenotype and susceptibility to Pseudomonas infection (129.24)
- Author
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Neely, Crystal J, primary, Wang, Ming-Jin, additional, Fuchs, Erin, additional, Vadlamudi, Sivaram, additional, Wolfgang, Matthew, additional, Cairns, Bruce A, additional, and Maile, Rob, additional
- Published
- 2009
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11. Th17 (IFNγ- IL17+) CD4+ T Cells Generated After Burn Injury May Be a Novel Cellular Mechanism for Postburn Immunosuppression.
- Author
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Neely, Crystal J., Maile, Robert, Wang, Ming-Jin, Vadlamudi, Sivaram, Meyer, Anthony A., and Cairns, Bruce A.
- Published
- 2011
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12. Catalytic Decomposition of N2O over M(10)/RPSA (M=Mg, Mn, Cu, Zn) Catalysts
- Author
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Wang, Ming Jin, Wang, Hong, Li, Cui Qing, Ding, Fu Cheng, Song, Yong Ji, and Cai, Ka Sha
- Abstract
Nitrous oxide (N
2 O) is a kind of important greenhouse gas,which has the potential damage on the ozone layer and people’s physical health. A series of RPSA loading metals catalysts were prepared by impregnation. The catalysts were characterized by XRD, TG-MS, NH3 -TPD, SEM method. The catalytic performance for N2 O decomposition was evaluated. Cu-based catalyst is the best activity for N2 O decomposition, T95 is 540°C.- Published
- 2014
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13. Th17 (IFN-IL17) CD4T Cells Generated After Burn Injury May Be a Novel Cellular Mechanism for Postburn Immunosuppression
- Author
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Neely, Crystal J., Maile, Robert, Wang, Ming-Jin, Vadlamudi, Sivaram, Meyer, Anthony A., and Cairns, Bruce A.
- Abstract
The mechanism responsible for initiating and controlling the immunosuppressive response after burn injury remains unknown. Interleukin-17 (IL-17) secreting Th17 (interferon IFN-IL17) cells are a novel subset of CD4T cells associated with a weak, proinflammatory response that antagonizes the proinflammatory Th1 (IFNIL17-) response. Given that transforming growth factor- and IL6 mediate Th17 cell development, we hypothesized that burn injury may generate Th17 cells that could mediate postburn immunosuppression.
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- 2011
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14. ACAT1 Induces the Differentiation of Glioblastoma Cells by Rewiring Choline Metabolism.
- Author
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You S, Wang MJ, Hou ZY, Wang WD, Zhang ZH, Du TT, Li SY, Liu YC, Xue NN, Hu XM, Chen XG, and Ji M
- Subjects
- Humans, Cell Line, Tumor, Animals, Mice, Astrocytes metabolism, Acetyl-CoA C-Acetyltransferase metabolism, Acetyl-CoA C-Acetyltransferase genetics, Glioblastoma metabolism, Glioblastoma pathology, Cell Differentiation, Choline metabolism, Choline pharmacology
- Abstract
Abnormal differentiation of cells is a hallmark of malignancy. Induction of cancer-cell differentiation is emerging as a novel therapeutic strategy with low toxicity in hematological malignances, but whether such treatment can be used in solid tumors is not known. Here, we uncovered a novel function of acetyl coenzyme A acetyltransferase (ACAT1) in regulating the differentiation of glioblastoma (GBM) cells. Inhibition of ACAT1 promoted the differentiation of GBM cells into astrocytes but also delayed tumor growth. Mechanistically, suppression of ACAT1 restored mitochondrial function and led to metabolic "reprogramming" in GBM cells: reduction of fatty-acid oxidation and acetyl-CoA, but an increase in free fatty acids. Importantly, ACAT1 negatively regulated the choline metabolic pathway, which is crucial for the differentiation of GBM cells. Finally, we demonstrated that a naturally available substance, chlorogenic acid (CHA), could inhibit phosphorylation of ACAT1 and so delay GBM progression, CHA is a promising candidate to treat GBM because it could induce the differentiation of cancer cells., Competing Interests: Competing Interests: The authors have declared that no competing interest exists., (© The author(s).)
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- 2024
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15. Prostate Cancer: Advances in Radiation Oncology, Molecular Biology, and Future Treatment Strategies
- Author
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Wang T, Lewis B, Ruscetti M, Mittal K, Wang MJ, Sokoloff M, Ding L, Bishop-Jodoin M, FitzGerald TJ, Barber N, and Ali A
- Abstract
Prostate cancer remains an important health problem worldwide affecting one in every six men including members of vulnerable communities. Although successful treatments have been delivered to men affected with the disease resulting in improved patient outcome, process improvements including therapy titration and augmentation are needed to optimize tumor control and limit normal tissue injury from therapy. In this chapter, we describe current management strategies for optimal patient care with radiation therapy and opportunities for improvement of care moving forward with applied science to apply therapy in a strategic manner, potentially improving care and outcome for patients treated for this disease., (Copyright: The Authors.; The authors confirm that the materials included in this chapter do not violate copyright laws. Where relevant, appropriate permissions have been obtained from the original copyright holder(s), and all original sources have been appropriately acknowledged or referenced.)
- Published
- 2022
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16. [Intestinal absorption characteristics of Polygonum orientale extract in normal and isoproterenol-induced myocardial ischemia model rats via everted intestinal sac models].
- Author
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Liu CH, Wang MJ, Yang ST, Li N, Lu Y, Pan J, Li YJ, Wang YL, and Sun J
- Subjects
- Animals, Intestinal Absorption, Intestines, Isoproterenol, Rats, Rats, Sprague-Dawley, Myocardial Ischemia chemically induced, Polygonum
- Abstract
The present study is to investigate the absorption characteristics of the main components in Polygonum orientale extract in normal and isoproterenol-induced myocardial ischemia model rats with everted intestinal sac models. Intestinal sac fluid samples were collected in different part of intestine(duodenum, jejunum, ileum, colon) at different time after administration of different concentration of P. orientale extract(5.0,10.0, 20.0 mg·mL~(-1)). An UPLC-TQD method was employed for the determination of six components including orientin, isoorientin, vitexin, protocatechuic acid, kaempferol-3-O-β-D-glucoside and quercitrin in the intestinal sac samples. The absorption rate and cumulative absorption were calculated to analyze the intestinal absorption characteristics of six components in normal and myocardial ischemia model rats. The P-glycoprotein(P-gp) inhibitor was applied to investigate influence of intestinal absorption of six components in P. orientale extract. The results showed that the main absorption sites were concentrated on the duodenum at low concentration, while they were the colon at the medium concentration and the ileum at high concentration in control groups. In the condition of myocardial ischemia model, the main absorption sites focus on the ileum and jejunum at low concentration; the main absorption sites were in the ileum at the medium concentration and main absorption sites were the duodenum and ileum at high concentration. Compared with the normal group, the absorption rate and cumulative absorption of the six components significantly decreased in the model group. P-gp inhibitor markedly increased the absorption rate and cumulative absorption of six components in the model group, inferring that the 6 components may be the substrates of P-gp, and the mechanism needs further study. In this study, it is revealed that the six components of P. orientale extract can be absorbed into the intestinal sac, and it is an effective method to assess the intestinal absorption characteristics of P. orientale extract through everted intestinal sac model, providing data support for the clinical application and further development of P. orientale.
- Published
- 2021
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