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2,140 results on '"Wang, Kemin"'

30. Cell Membrane-Anchored AND Logic Gate Aptasensor for Tumor Cell-Specific Imaging with Improved Accuracy

Author

Chen, Biao, Qi, Lanlin, Wu, Yuchen, Chen, Mingjian, Zhou, Yan, He, Lin, Zhang, Bin, Zhang, Min, Wang, Kemin, and He, Xiaoxiao

Abstract

Accurate and rapid imaging of tumor cells is of vital importance for early cancer diagnosis and intervention. Aptamer-based fluorescence sensors have become a potent instrument for bioimaging, while false positives and on-target off-tumors linked to single-biomarker aptasensors compromise the specificity and sensitivity of cancer imaging. In this paper, we describe a sequential response aptasensor for precise cancer cell identification that is based on a DNA “AND” logic gate. Specifically, the sensor consists of three single-stranded DNA, including the P-strand that can sensitively respond to an acid environment, the L-strand containing the ATP aptamer sequence, and the R-strand for target cell anchoring. These DNA strands hybridize with one another to create a Y-shaped structure (named Y-ALGN). The aptamer in the R-strand is utilized to anchor the sensor to the target cell membrane primarily. Responding to the extracellular acidic environment of the tumor (input 1), the I-motif sequence forms a tetramer structure so that the P-strand is released from the Y-shaped structure and exposes the ATP binding sites in the L-strand. Extracellular ATP, as input 2, continuously operates the DNA aptasensor to complete the logic computation. Upon the sequential response of both protons and ATP molecules, the aptasensor is activated with restored fluorescence on a particular cancer cell membrane. Benefiting from the precise computation capacity of the “AND” logic gate, the Y-ALGN aptasensor can distinguish between MCF-7 cells and normal cells with high accuracy. As a simple and dual-stimuli-responsive strategy, this nanodevice would offer a fresh approach for accurately diagnosing tumor cells.

Published
2024
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39. The Formation and Development of Chinese EFL Learners' English Mental Lexicon

Author

Dai, Zhongxin and Wang, Kemin

Abstract

This paper investigated some dominant features and influential factors in the formation and developmental process of the English mental lexicon of Chinese EFL learners. Data were collected by means of word tests. The result of the data analysis shows: (1) Chinese EFL learners acquire very little of the overall word knowledge; and (2) aspects of the multifaceted word knowledge increase with the improvement of their language proficiency.

Published
2013

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