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1,321 results on '"Wang, Jianbin"'

9. Corrosion Performance of Low Carbon Steels Containing Copper in Cl- Environment with Different pH Values

Author

WANG Jianbin, XU Xiuting, XUE Yunsheng, LIU Hongyu, HAI Guoquan, GUO Jing, TENG Yingxue

Subjects
cl- environment, ac impedance, ph, polarization curve, Materials of engineering and construction. Mechanics of materials, TA401-492, Technology
Abstract

For exploring the corrosion rules of copper-containing low carbon steel under different pH conditions，the electrochemical workstation and metallographic microscope were used to analyze the polarization curves，AC impedance spectra and micro morphologies of ordinary weathering steel (Q345)，copper-containing low carbon steel (0Cu3Cr) and hull copper-containing low carbon steel (0CuAl) in NaCl environments with different pH values.Additionally，the difference in corrosion performance between copper-containing steel and low carbon steel in NaCl environments with different pH values was observed.Results showed the self-corrosion potential of the three samples was higher in the NaCl environment with pH＝12.The corrosion processes of 0Cu3Cr and 0CuAl were controlled by the anodic dissolution reaction，while that of Q345 was controlled by the cathodic reaction.Moreover，the self-corrosion potential of Q345 and 0Cu3Cr at pH ＝6 was slightly greater than that at pH＝8.The charge transfer resistance values of the three kinds of samples were the largest in the NaCl environment with pH＝12.When pH＝6，the pitting pits on the surface of 0Cu3Cr were smaller and less numerous.When pH＝8，the pitting pits on the surface were larger and more numerous.Besides，the corrosion resistance of 0CuAl at pH＝8 was better than that at pH＝6.

Published
2024
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38. Analytical Methods for In-Process Testing and Product Release

Author

Haig, Neil A., primary, Valeich, Jamie E., additional, DeMaster, Laura, additional, Do, Christopher, additional, Marculescu, Razvan, additional, Wang, Jianbin, additional, Fisher, Bridget S., additional, Wu, Xiaoping, additional, Ijantkar, Akshata, additional, Rodia, Cayla N., additional, Madsen, Sean D., additional, Ibrahim, Mohammed, additional, Davis-Pickett, Mel, additional, Hu, Jennifer F., additional, Robbins, Jennifer J., additional, Powell, James Collins, additional, Shrivastava, Divya, additional, and Singh, Satyakam, additional

Published
2023
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49. RNA sequencing by direct tagmentation of RNA/DNA hybrids.

Author

Di, Lin, Fu, Yusi, Sun, Yue, Li, Jie, Liu, Lu, Yao, Jiacheng, Wang, Guanbo, Wu, Yalei, Lao, Kaiqin, Lee, Raymond, Zheng, Genhua, Xu, Jun, Oh, Juntaek, Wang, Dong, Xie, X, Huang, Yanyi, and Wang, Jianbin

Subjects
RNA-seq, Tn5 transposase, single cell, Chimera, DNA, DNA, Complementary, Gene Library, HEK293 Cells, HeLa Cells, Humans, RNA, Sequence Analysis, RNA, Single-Cell Analysis, Transposases
Abstract

Transcriptome profiling by RNA sequencing (RNA-seq) has been widely used to characterize cellular status, but it relies on second-strand complementary DNA (cDNA) synthesis to generate initial material for library preparation. Here we use bacterial transposase Tn5, which has been increasingly used in various high-throughput DNA analyses, to construct RNA-seq libraries without second-strand synthesis. We show that Tn5 transposome can randomly bind RNA/DNA heteroduplexes and add sequencing adapters onto RNA directly after reverse transcription. This method, Sequencing HEteRo RNA-DNA-hYbrid (SHERRY), is versatile and scalable. SHERRY accepts a wide range of starting materials, from bulk RNA to single cells. SHERRY offers a greatly simplified protocol and produces results with higher reproducibility and GC uniformity compared with prevailing RNA-seq methods.

Published
2020

50. USP14 regulates cell cycle progression through deubiquitinating CDK1 in breast cancer

Author

Liu Yuhan, Xu Jing, Wang Yanan, Gan Mingxi, Hu Qifan, Wang Jianbin, and Han Tianyu

Subjects
breast cancer, USP14, CDK1, deubiquitination, Biochemistry, QD415-436, Genetics, QH426-470
Abstract

Abnormal proliferation and cell cycle perturbation are the main hallmarks of breast cancer. Cyclin-dependent kinase 1 (CDK1) is one of the key kinases for cell transition from the G2 phase to M phase during the cell cycle progression. However, little is known about the degradation mechanisms of CDK1. USP14 (ubiquitin-specific processing protease 14) is an important proteasome-associated deubiquitinase that is critical for proteome homeostasis and plays a crucial role in the initiation and development of cancer. In this study, we find that USP14 shows high expression in breast cancer cells and results in the abnormal proliferation of cancer cells. Furthermore, we examine cell cycle distribution by flow cytometry and find that inhibition of USP14 causes cell cycle arrest in G2/M phase. As CDK1 is the key kinase in G2/M phase, we detect the interaction between USP14 and CDK1 and the effect of USP14 on the deubiquitination of CDK1. The results reveal that USP14 interacts with CDK1 and stabilizes CDK1 by deubiquitinating K48-linked ubiquitination. In conclusion, our findings reveal an indispensable role of USP14 in regulating cell cycle progression by stabilizing CDK1 in breast cancer, suggesting that USP14 may be used as a potential therapeutic target in breast cancer therapy.

Published
2022
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