1. Knockdown of CYP301B1 and CYP6AX1v2 increases the susceptibility of the brown planthopper to beta-asarone, a potential plant-derived insecticide
- Author
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Xu Xueliang, Liu Zirong, Fan Linjuan, Caiyun Wu, Wang Fenshan, Li Xiang, and Yao Yingjuan
- Subjects
Insecticides ,medicine.medical_specialty ,Zygote ,Fat Body ,Allylbenzene Derivatives ,02 engineering and technology ,Anisoles ,Biochemistry ,Hemiptera ,03 medical and health sciences ,Cytochrome P-450 Enzyme System ,Structural Biology ,RNA interference ,Internal medicine ,medicine ,Animals ,Gene silencing ,Amino Acid Sequence ,RNA, Small Interfering ,Molecular Biology ,Gene ,030304 developmental biology ,0303 health sciences ,Gene knockdown ,Base Sequence ,biology ,Cytochrome P450 ,General Medicine ,Monooxygenase ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Intestines ,Endocrinology ,Gene Expression Regulation ,Inactivation, Metabolic ,biology.protein ,Insect Proteins ,Integument ,Brown planthopper ,0210 nano-technology ,Head - Abstract
The cytochrome P450 monooxygenases of insects play crucial roles in the metabolic detoxification of insecticides. Our previous finding showed that two cytochrome P450 genes, both CYP301B1 and CYP6AX1v2, in the BPH underwent overexpression due to β-asarone. In this study, we investigated the molecular characteristics, expression patterns and functions of these two cytochrome P450 genes. The results showed that CYP301B1 had the highest expression level in the eggs, while CYP6AX1v2 was expressed in macropterous female adults. Moreover, the expression level of CYP301B1 in the head was higher than that in the integument, fat body and gut. The expression level of CYP6AX1v2 in the fat body and gut was higher than that in head and integument. Importantly, silencing CYP301B1 and CYP6AX1v2 separately could increase the sensitivity, resulting in significant higher mortality of BPH following treatment with β-asarone. Our findings indicated that CYP301B1 and CYP6AX1v2 could contribute to the resistance of BPH to β-asarone, and these two genes may be involved in the detoxification metabolism of β-asarone in BPH.
- Published
- 2021