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11 results on '"Wang, Daying"'

3. Dalbergia odorifera extract promotes angiogenesis through upregulation of VEGFRs and PI3K/MAPK signaling pathways

Author

Youhua Wang, Zhu-Ming Fan, Liu Zongjun, Zhi-Xiu Lin, Ai-Lin Yang, Xin Zhou, Jian-Mei Yang, Yun-Xiao Lin, Su-yun Yuan, Min Cao, Hua Fan, and Wang Daying

Subjects
0301 basic medicine, MAPK/ERK pathway, Angiogenesis, Dalbergia, Neovascularization, Physiologic, Biology, Animals, Genetically Modified, Phosphatidylinositol 3-Kinases, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Western blot, Cell Movement, In vivo, Drug Discovery, Human Umbilical Vein Endothelial Cells, medicine, Animals, Humans, Therapeutic angiogenesis, Zebrafish, Cells, Cultured, PI3K/AKT/mTOR pathway, Cell Proliferation, Pharmacology, medicine.diagnostic_test, Plant Extracts, biology.organism_classification, Molecular biology, Cell biology, Receptors, Vascular Endothelial Growth Factor, 030104 developmental biology, 030220 oncology & carcinogenesis, cardiovascular system, Angiogenesis Inducing Agents, Mitogen-Activated Protein Kinases, Signal Transduction
Abstract

Ethnopharmacological relevance The heart wood of Dalbergia odorifera is a Chinese herbal medicine commonly used for the treatment of various ischemic diseases in Chinese medicine practice. Aim of the study In this study, therapeutic angiogenesis effects of the Dalbergia odorifera extract (DOE) were investigated on transgenic zebrafish in vivo and human umbilical vein endothelial cells (HUVECs) in vitro. Materials and methods The pro-angiogenic effects of DOE on zebrafish were examined by subintestinal vessels (SIVs) sprouting assay and intersegmental vessels (ISVs) injury assay. And the pro-angiogenic effects of DOE on HUVECs were examined by MTT, scratch assay, protein chip and western blot. Results In the in vivo studies, we found that DOE was able to dose-dependently promote angiogenesis in zebrafish SIVs area. In addition, DOE could also restore the injury in zebrafish ISVs area and upregulate the reduced mRNA expression of VEGFRs including kdr, kdrl and flt-1 induced by VEGF receptor kinase inhibitor II (VRI). In the in vitro studies, we observed that DOE promoted the proliferation, migration of HUVECs and also restored the injury induced by VRI. Moreover, protein chip and western blot experiments showed the PI3K/MAPK cell proliferation/migration pathway were activated by DOE. Conclusions DOE has a therapeutic effects on angiogenesis, and its mechanism may be related to adjusting the VEGFRs mRNA and activation of PI3K/MAPK signaling pathway. These results suggest a strong potential for Dalbergia odorifera to be developed as an angiogenesis-promoting therapeutic.

Published
2017

6. HEDD: the human epigenetic drug database

Author

Qi, Yunfeng, primary, Wang, Dadong, additional, Wang, Daying, additional, Jin, Taicheng, additional, Yang, Liping, additional, Wu, Hui, additional, Li, Yaoyao, additional, Zhao, Jing, additional, Du, Fengping, additional, Song, Mingxia, additional, and Wang, Renjun, additional

Published
2016
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11. [Immunosuppressive action of dendritic cells sensitized by oligodeoxynucleotides and CA125 on human OVCAR3 ovarian carcinoma xenografts in nude mice].

Author

Ye M, Li N, Huang X, Wang D, and Shang J

Subjects
Animals, Apoptosis drug effects, Carcinoma, Ovarian Epithelial, Cell Line, Tumor, Female, Humans, Immunotherapy, Mice, Mice, Nude, Xenograft Model Antitumor Assays, CA-125 Antigen immunology, Dendritic Cells immunology, Neoplasms, Glandular and Epithelial therapy, Oligodeoxyribonucleotides immunology, Ovarian Neoplasms therapy
Abstract

Objective: To explore the immunosuppressive action of dendritic cells (DC) sensitized by oligodeoxynucleotides containing "un-methylated cytosine phosphodiester bond-guanylic acid" motif (CpG ODN) and CA125 on human ovarian carcinoma xenografts in nude mice., Methods: Human peripheral blood-derived dendritic cells were isolated and identified by flow cytometry. The DC sensitized by CpG ODN and CA125 were then co-cultured with T cells and finally cytotoxic T lymphocytes (CTL) were induced. Nude mice bearing OVCAR3 transplanted tumor were immunized with induced CTL by subcutaneous injection and tumor growth and cell apoptosis observed., Results: Premature DC had a low expression of CD83 and CD86. In vivo, delayed growth of OVCAR3 xenografts was observed after immunotherapy with CTL induced by DC pulsed by CpG ODN and CA125. The inhibition rate of tumor was 50.71% and it was better than CpG ODN-pulsed and CA125-pulsed groups (P < 0.05). Cell apoptotic rate was (29.6 ± 3.0)% in CpG ODN+CA125-pulsed group versus (21.8 ± 2.7)% in CpG ODN-pulsed group. And both were more than those of unpulsed and CA125-pulsed groups (P < 0.05)., Conclusion: CTL induced by DC sensitized by CpG ODN and CA125 can inhibit the growth of ovarian carcinoma xenografts and promote tumor cell apoptosis.

Published
2014

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