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17. Integrating metabolomics and network pharmacology to assess the effects of Mahuang Xixin Fuzi decoction on migraine rats induced by nitroglycerin.

Author

Ge, Fei, Zhang, Yao, Luo, Yamin, Wang, Chunguo, Lu, Yixing, Zhao, Yafang, Zhang, Di, Meng, Fengxian, Zhang, Dongmei, Chen, Meng, and Tao, Xiaohua

Subjects
MIGRAINE, METABOLOMICS, LABORATORY rats, SUMATRIPTAN, RATS, NITROGLYCERIN, SPREADING cortical depression
Abstract

Objectives: This study was designed to investigate the pharmacological activity and therapeutic mechanism of Mahuang Xixin Fuzi decoction (MXFD) on migraine. Methods: Migraine model rats induced by nitroglycerin were established, and then orally administered with MXFD for 7 days. Blood and urine samples were collected to identify differential metabolites with metabolomics. To integrate the findings from network pharmacology and metabolomics analysis, the metabolites and targets related to MXFD therapy for migraine were filtered. Key findings: MXFD was found to alleviate the symptoms of migraines in rats. After treatment with MXFD, nine metabolites were found to be regulated and returned to normal levels. MXFD acted directly on nine key targets including MAOB, MAOA, ADRB1, ADRB2, ADRB3, ADORA2A, ADORA2B, DRD5, and HTR4 and regulated two out of nine metabolites, namely deoxycholic acid and 5-methoxyindoleacetate. Conclusions: The study found that MXFD can alleviate migraines through multitarget and multicomponent interaction networks. Graphical Abstract [ABSTRACT FROM AUTHOR]

Published
2024
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29. Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in Rice

Author

Wang Bo, Zhong Zhaohui, Zhang Huanhuan, Wang Xia, Liu Binglin, Yang Lijia, Han Xiangyan, Yu Deshui, Zheng Xuelian, Wang Chunguo, Song Wenqin, Chen Chengbin, and Zhang Yong

Subjects
Plant culture, SB1-1110
Abstract

Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt stress with various metabolism pathways. A number of plant transcription factor family genes have been found to be involved in the salt stress response, and NAM, ATAF and CUC (NAC) transcription factors are thought to act as active regulators during abiotic stress, especially salt stress. In this study, we detected a rice NAC transcription factor coding gene, OsNAC041, and confirmed that it influenced the germination of seeds under salt stress and salt tolerance of plants. OsNAC041 was primarily expressed in the leaves and located in the nucleus. Furthermore, the CRISPR/Cas9 method was used to obtain a targeted osnac041 mutant, of which the plant height was higher than that of the wild-type, showing increased salt sensitivity. Moreover, RNA-seq analysis revealed a number of differentially expressed genes (DEGs) involved in several important signaling pathways in the osnac041 mutant. Subsequently, Kyoto Encyclopedia of Genes and Genomes annotation also revealed differential expression of DEGs associated with mitogen-activated protein kinase signaling, peroxisome, eukaryotic- type ABC transporters, photosynthesis and plant hormones, which are involved in stress-related signaling pathways. Overall, our study suggested that OsNAC041 was involved in the salt stress response in rice. These findings not only provide empirical evidence of OsNAC041 function, but also provide new insight into its potential application in rice resistance breeding. Keywords: rice, NAC transcription factor, CRISPR, Cas9, RNA-seq, salinity

Published
2019
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