1. Retinal perivascular macrophages regulate immune cell infiltration during neuroinflammation in mouse models of ocular disease
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Sterling, Jacob K., Rajesh, Amrita, Droho, Steven, Gong, Joyce, Wang, Andrew L., Voigt, Andrew P., Brookins, C. Elysse, and Lavine, Jeremy A.
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Physiological aspects ,Models ,Health aspects ,Macrophages -- Health aspects ,Retina -- Health aspects ,Inflammation -- Physiological aspects ,Medical research ,Eye diseases -- Physiological aspects -- Models ,Medicine, Experimental - Abstract
Introduction The blood vessels of the CNS possess unique properties that allow them to regulate the flux of molecules and cells between the systemic circulation and the tightly controlled environment [...], The blood-retina barrier (BRB), which is disrupted in diabetic retinopathy (DR) and uveitis, is an important anatomical characteristic of the retina, regulating nutrient, waste, water, protein, and immune cell flux. The BRB is composed of endothelial cell tight junctions, pericytes, astrocyte end feet, a collagen basement membrane, and perivascular macrophages. Despite the importance of the BRB, retinal perivascular macrophage function remains unknown. We found that retinal perivascular macrophages resided on postcapillary venules in the superficial vascular plexus and expressed MHC class II. Using single-cell RNA-Seq, we found that perivascular macrophages expressed a prochemotactic transcriptome and identified platelet factor 4 (Pf4, also known as CXCL4) as a perivascular macrophage marker. We used [Pf4.sup.Cre] mice to specifically deplete perivascular macrophages. To model retinal inflammation, we performed intraocular CCL2 injections. [Ly6C.sup.+] monocytes crossed the BRB proximal to perivascular macrophages. Depletion of perivascular macrophages severely hampered [Ly6C.sup.+] monocyte infiltration. These data suggest that retinal perivascular macrophages orchestrate immune cell migration across the BRB, with implications for inflammatory ocular diseases including DR and uveitis.
- Published
- 2024
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