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Transient conjugate heat transfer measurements under varying temperature and velocity inlet boundary conditions at incompressible flow conditions were performed for flat plate and ribbed channel geometries. Therefrom, local adiabatic wall temperatures and heat transfer coefficients were determined. The data were analyzed using typical heat transfer correlations, e.g., Nu = C Re m Pr n , determining the local distributions of C and m. It is shown that they are closely linked. A relationship ln C = A − m B is observed, with A and B as modeling parameters. They could be related to parameters in log-law or power-law representations for turbulent boundary layer flows. The parameter m is shown to have a close link to local pressure gradients and, therewith, near wall streamlines as well as friction factor distributions. A normalization of the C parameter allows one to derive a Reynolds analogy factor and, therefrom, local wall shear stresses. [ABSTRACT FROM AUTHOR]

STUDY QUESTION Can a co-culture of three cell types mimic the in vivo layers of the uterine wall? SUMMARY ANSWER Three protocols tested for co-culture of endometrial epithelial cells (EEC), endometrial stromal cells (ESC), and myometrial smooth muscle cells (MSMC) led to formation of the distinct layers that are characteristic of the structure of the uterine wall in vivo. WHAT IS KNOWN ALREADY We previously showed that a layer-by-layer co-culture of EEC and MSMC responded to peristaltic wall shear stresses (WSS) by increasing the polymerization of F-actin in both layers. Other studies showed that WSS induced significant cellular alterations in epithelial and endothelial cells. STUDY DESIGN, SIZE, DURATION Human EEC and ESC cell lines and primary MSMC were co-cultured on a collagen-coated synthetic membrane in custom-designed wells. The co-culture model, created by seeding a mixture of all cells at once, was exposed to steady WSS of 0.5 dyne/cm2 for 10 and 30 min. PARTICIPANTS/MATERIALS, SETTING, METHODS The co-culture of the three different cells was seeded either layer-by-layer or as a mixture of all cells at once. Validation of the models was by specific immunofluorescence staining and confocal microscopy. Alterations of the cytoskeletal F-actin in response to WSS were analyzed from the 2-dimensional confocal images through the Z-stacks following a previously published algorithm. MAIN RESULTS AND THE ROLE OF CHANCE We generated three multi-cell in vitro models of the uterine wall with distinct layers of EEC, ESC, and MSMC that mimic the in vivo morphology. Exposure of the mixed seeding model to WSS induced increased polymerization of F-actin in all the three layers relative to the unexposed controls. Moreover, the increased polymerization of F-actin was higher (P -value < 0.05) when the length of exposure was increased from 10 to 30 min. Furthermore, the inner layers of ESC and MSMC, which are not in direct contact with the applied shearing fluid, also increased their F-actin polymerization. LARGE SCALE DATA N/A. LIMITATIONS, RESONS FOR CAUTION The mixed seeding co-culture model was exposed to steady WSS of one magnitude, whereas the uterus is a dynamic organ with intra-uterine peristaltic fluid motions that vary in vivo with different time-dependent magnitude. Further in vitro studies may explore the response to peristaltic WSS or other physical and/or hormonal perturbations that may mimic the spectrum of pathophysiological aspects. WIDER IMPLICATIONS OF THE FINDINGS Numerous in vitro models were developed in order to mimic the human endometrium and endometrium–myometrium interface (EMI) region. The present co-culture models seem to be the first constructed from EEC, ESC, and MSMC on a collagen-coated synthetic membrane. These multi-cell in vitro models better represent the complex in vivo anatomy of the EMI region. The mixed seeding multi-cell in vitro model may easily be implemented in controlled studies of uterine function in reproduction and the pathogenesis of diseases. STUDY FINDING/COMPETING INTEREST(S) This study was supported in part by Tel Aviv University funds. All authors declare no conflict of interest. [ABSTRACT FROM AUTHOR]

Transient conjugate heat transfer measurements under varying temperature and velocity inlet boundary conditions at incompressible flow conditions were performed for flat plate and ribbed channel geometries. Therefrom, local adiabatic wall temperatures and heat transfer coefficients were determined. The data were analyzed using typical heat transfer correlations, e.g., Nu=CRemPrn, determining the local distributions of C and m. It is shown that they are closely linked. A relationship lnC=A−mB is observed, with A and B as modeling parameters. They could be related to parameters in log-law or power-law representations for turbulent boundary layer flows. The parameter m is shown to have a close link to local pressure gradients and, therewith, near wall streamlines as well as friction factor distributions. A normalization of the C parameter allows one to derive a Reynolds analogy factor and, therefrom, local wall shear stresses.

Carotid artery diseases, such as atherosclerosis, are a major cause of death in the United States. Wall shear stresses are known to prompt plaque formation, but there is limited understanding of the complex flow structures underlying these stresses and how they differ in a pre-disposed high-risk patient cohort. A 'healthy' and a novel 'pre-disposed' carotid artery bifurcation model was determined based on patient-averaged clinical data, where the 'pre-disposed' model represents a pathological anatomy. Computational fluid dynamic simulations were performed using a physiological flow based on healthy human subjects. A main hairpin vortical structure in the internal carotid artery sinus was observed, which locally increased instantaneous wall shear stress. In the pre-disposed geometry, this vortical structure starts at an earlier instance in the cardiac flow cycle and persists over a much shorter period, where the second half of the cardiac cycle is dominated by perturbed secondary flow structures and vortices. This coincides with weaker favorable axial pressure gradient peaks over the sinus for the 'pre-disposed' geometry. The findings reveal a strong correlation between vortical structures and wall shear stress and imply that an intact internal carotid artery sinus hairpin vortical structure has a physiologically beneficial role by increasing local wall shear stresses. The deterioration of this beneficial vortical structure is expected to play a significant role in atherosclerotic plaque formation. [ABSTRACT FROM AUTHOR]

Purpose: In this work we performed an imaged-based computational study of the systolic fluid dynamics in presence of mitral valve regurgitation (MVR). In particular, we compared healthy and different regurgitant scenarios with the aim of quantifying different hemodynamic quantities. Methods: We performed computational fluid dynamic (CFD) simulations in the left ventricle, left atrium and aortic root, with a resistive immersed method, a turbulence model, and with imposed systolic wall motion reconstructed from Cine-MRI images, which allowed us to segment also the mitral valve. For the regurgitant scenarios we considered an increase of the heart rate and a dilation of the left ventricle. Results: Our results highlighted that MVR gave rise to regurgitant jets through the mitral orifice impinging against the atrial walls and scratching against the mitral valve leading to high values of wall shear stresses (WSSs) with respect to the healthy case. Conclusion: CFD with prescribed wall motion and immersed mitral valve revealed to be an effective tool to quantitatively describe hemodynamics in case of MVR and to compare different regurgitant scenarios. Our findings highlighted in particular the presence of transition to turbulence in the atrium and allowed us to quantify some important cardiac indices such as cardiac output and WSS. [ABSTRACT FROM AUTHOR]

Carotid artery diseases, such as atherosclerosis, are a major cause of death in the United States. Wall shear stresses are known to prompt plaque formation, but there is limited understanding of the complex flow structures underlying these stresses and how they differ in a pre-disposed high-risk patient cohort. A ‘healthy’ and a novel ‘pre-disposed’ carotid artery bifurcation model was determined based on patient-averaged clinical data, where the ‘pre-disposed’ model represents a pathological anatomy. Computational fluid dynamic simulations were performed using a physiological flow based on healthy human subjects. A main hairpin vortical structure in the internal carotid artery sinus was observed, which locally increased instantaneous wall shear stress. In the pre-disposed geometry, this vortical structure starts at an earlier instance in the cardiac flow cycle and persists over a much shorter period, where the second half of the cardiac cycle is dominated by perturbed secondary flow structures and vortices. This coincides with weaker favorable axial pressure gradient peaks over the sinus for the ‘pre-disposed’ geometry. The findings reveal a strong correlation between vortical structures and wall shear stress and imply that an intact internal carotid artery sinus hairpin vortical structure has a physiologically beneficial role by increasing local wall shear stresses. The deterioration of this beneficial vortical structure is expected to play a significant role in atherosclerotic plaque formation.

We numerically study the fluid dynamics of oil tamponade in models of vitrectomized eyes prompted by a subset of daily activities corresponding to movements on the horizontal plane with the patient in a standing position. Bulk flow features are related to near-wall flow topology and transport at the retinal surface through a wall shear-stress-based analysis. Proliferative VitreoRetinopathy (PVR) is the leading cause of retinal re-detachment occurring in about 20% of all cases due to the accumulation of inflammatory cells in discrete retinal regions. Signalling soluble mediators stimulate inflammatory cells' chemotaxis and studying their distribution across the retinal surface may acquire clinical relevance. In all the investigated cases, persistent and elongated regions along the retina, potentially prone to accumulate chemo-attractants and cells are observed. Gradients of soluble inflammation mediators present in the aqueous are known responsible for the so-called epithelial-mesenchymal transition that initiates PVR and favours recurrent retinal detachment prompting the proliferation of inflammatory cells with collagen matrix deposition and its contraction. The surgical apposition of encircling scleral buckling elements, known for over a century to influence PVR formation and localization, modifies the attracting regions, possibly causing an accumulation of molecules and cells along approximately vertical lines that follow the rising menisci due to the cerclage indentation. The resulting spatial pattern is compatible with clinical observations. This study may open toward rational analyses of near-wall transport to predict PVR pathogenesis by relating biochemical accumulation in certain areas of the retina to clinical conditions. [ABSTRACT FROM AUTHOR]

Vegetation exists on the floodplains of almost all natural channels. This vegetation results in a number of changes in flow features of open channel flows. Sometimes vegetation also exists within the main channel. This paper presents a numerical study in which the impact of vegetation density on different flow features has been considered. The variables investigated included velocity profiles, bed shear stresses, Reynolds stresses and side wall shear stresses. A compound channel with floodplain on both sides of the main channel has been considered. Four different vegetation densities were considered in this work. It was revealed that increase in vegetation density results in a decrease in the velocity values close to the bed and in the upper regions of the flood plains. The Reynolds stresses were also influenced considerably in the lower regions of the channel. Similar patterns were observed in case of bed shear stresses. The vertical profiles of side wall shear stresses were also investigated in this work. Such an investigation will help in enhancing the understanding and improving different conveyance and flow resistance calculation formulae.

Aneurysms are abnormal expansion of weakened blood vessels which can be debilitating or fatal upon rupture. Previous studies have shown that hemodynamics play a role in aneurysm formation, growth and rupture. Therefore, the objective of this work is to investigate the impact of flow structures on vortex strength, impinging location, and wall shear stress for two aneurysm models. For this study, experiments were performed on two different, idealized, rigid, and saccular aneurysm models characterized by B F ratios of 1.0 and 1.6. The vortical structures formation and movement were altered by changing peak Reynolds number (R e p ) from 50 and 270 and Womersley number (α) from 2, and 5 on both aneurysm models. Velocity field measurements were performed on the mid-plane location of the aneurysm models using Particle Image Velocimetry (PIV). The results showed presence of vortical structures which formed, grew, and dissipated at different phases in the flow cycle. The behavior of these vortical structures impacted the impinging locations where increased frequency of impingement points at the distal neck could be seen for α = 5 than α = 2 flow scenarios. The wall shear stress results showed that the distal neck experienced elevated stresses during the vortex formation phases of the flow cycle for B F = 1. 0 and 1.6 models. • Reynolds number influences the flow structures in the saccular aneurysm model. • Flow structures play a role in the impinging location. • Flow structures play a role in modifying the wall shear stresses. • For high Reynolds number impinging locations are concentrated near the distal neck. [ABSTRACT FROM AUTHOR]

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The aim of the present study is to characterize wall shear stresses generated by a gas-liquid two-phase flow inside a capillary tube. In this case the flow is a slug flow. Two different approaches were used. The first one constituted a calculation of the shear stresses by a two-phase model in which some parameters characteristics of the flow were introduced. These parameters had been determined experimentally in a previous study. The second approach constituted a direct and local measurement of wall shear stresses by an electrochemical method. The shear stresses near liquid and gas slugs were obtained for different operating conditions. Moreover, a calculation of the liquid film thickness around the gas slugs demonstrated that this thickness does not depend on gas velocity. [ABSTRACT FROM AUTHOR]

Hydraulic transportation of the piped carriage is a new energy-saving and environmentally-friendly transportation mode. There are two main states in the conveying process, stationary and moving. In the process of hydraulic transportation of the piped carriage, the study of the stress of the water flow act on the cylinder wall of the piped carriage can help to improve the design of the piped carriage structure and even the selection of piped carriage materials. The distribution of flow velocity around the stationary piped carriage and the stress distribution on the cylinder wall of the stationary piped carriage were investigated by combining numerical simulations with model experiment verification. The commercial finite element software, Comsol Multiphysics, was utilized to solve this problem using the arbitrary Lagrangian–Eulerian (ALE) method. The results showed that the simulation results were in good agreement with the experimental results. It also showed that the ALE method can well be applied for fluid-structure problems in the process of hydraulic transportation of the piped carriage. The simulation results showed that the low velocity region near the inner wall of the pipe was smaller than that near the outer wall of the piped carriage, and both regions decreased with the increase of the discharge. The maximum stress on the cylinder wall of the piped carriage appeared between the two support feet in the middle and rear sections of the cylinder. The influence of the unit discharge on wall stress increased with the increase of the discharge, that is, k1 < k2 < k3. Moreover, the increase of the discharge had the greatest influence on the circumferential component of the principal stress of the cylinder, followed by the axis component, and the smallest influence on the wall shear stress of the cylinder, i.e., k ¯ σ c > k ¯ σ a > k ¯ σ r > k ¯ τ c . [ABSTRACT FROM AUTHOR]