17 results on '"Walker WB 3rd"'
Search Results
2. Using eDNA to play whack-a-mole with invasive species in green yard waste.
- Author
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Neven LG, Walker WB 3rd, Gowton C, and Carrillo J
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- Animals, Washington, Insecta genetics, British Columbia, Waste Disposal Facilities, DNA Barcoding, Taxonomic, Introduced Species, DNA, Environmental analysis
- Abstract
As large cities begin to overrun their landfill capacities, they begin to look for alternative locations to handle the waste stream. Seeing an opportunity to bring in revenue, rural communities offer to handle municipal waste in their landfills. However, many rural communities are also places of agricultural production, which are vulnerable to attacks by invasive insect species, which could be present in green yard waste, the component of municipal waste most likely to contain agriculturally harmful insect species. We used environmental DNA (eDNA) to determine whether green yard waste could be a pathway for invasive insect species to enter and establish in the landfill-receiving agricultural community. We identified several target species that could be in green yard waste coming from Vancouver, BC, Canada, to Central Washington State, USA. We sampled green yard waste from 3 sites every 2 weeks from June to October in 2019 and 2020. DNA was extracted from the nearly 400 samples and subjected to amplification with COI barcoding primers followed by sequencing to identify target insects in the samples. Sequence analyses identified 3 species from the target list: 2 species that are pests of deciduous tree fruits and a generalist root-feeding crop pest. This eDNA technique was useful in identifying potential invasive species in green yard waste and may prove to be an important tool informing policy on the movement of biological material across borders and stemming the spread of invasive species., (Published by Oxford University Press on behalf of Entomological Society of America 2024.)
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- 2024
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3. Comparative transcriptomic assessment of the chemosensory receptor repertoire of Drosophila suzukii adult and larval olfactory organs.
- Author
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Walker WB 3rd, Mori BA, Cattaneo AM, Gonzalez F, Witzgall P, and Becher PG
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- Female, Male, Animals, Larva genetics, Drosophila melanogaster genetics, Gene Expression Profiling, Drosophila genetics, Drosophila metabolism, Transcriptome
- Abstract
The spotted wing Drosophila, Drosophila suzukii, has emerged within the past decade as an invasive species on a global scale, and is one of the most economically important pests in fruit and berry production in Europe and North America. Insect ecology, to a strong degree, depends on the chemosensory modalities of smell and taste. Extensive research on the sensory receptors of the olfactory and gustatory systems in Drosophila melanogaster provide an excellent frame of reference to better understand the fundamentals of the chemosensory systems of D. suzukii. This knowledge may enhance the development of semiochemicals for sustainable management of D. suzukii, which is urgently needed. Here, using a transcriptomic approach we report the chemosensory receptor expression profiles in D. suzukii female and male antennae, and for the first time, in larval heads including the dorsal organ that houses larval olfactory sensory neurons. In D. suzukii adults, we generally observed a lack of sexually dimorphic expression levels in male and female antennae. While there was generally conservation of antennal expression of odorant and ionotropic receptor orthologues for D. melanogaster and D. suzukii, gustatory receptors showed more distinct species-specific profiles. In larval head tissues, for all three receptor gene families, there was also a greater degree of species-specific gene expression patterns. Analysis of chemosensory receptor repertoires in the pest species, D. suzukii relative to those of the genetic model D. melanogaster enables comparative studies of the chemosensory, physiology, and ecology of D. suzukii., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Published by Elsevier Inc.)
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- 2023
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4. Author Correction: Antennal transcriptome sequencing and identification of candidate chemoreceptor proteins from an invasive pest, the American palm weevil, Rhynchophorus palmarum.
- Author
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Gonzalez F, Johny J, Walker WB 3rd, Guan Q, Mfarrej S, Jakše J, Montagné N, Jacquin-Joly E, Alqarni AS, Al-Saleh MA, Pain A, and Antony B
- Published
- 2021
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5. Antennal transcriptome sequencing and identification of candidate chemoreceptor proteins from an invasive pest, the American palm weevil, Rhynchophorus palmarum.
- Author
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Gonzalez F, Johny J, Walker WB 3rd, Guan Q, Mfarrej S, Jakše J, Montagné N, Jacquin-Joly E, Alqarni AS, Al-Saleh MA, Pain A, and Antony B
- Subjects
- Animals, Asia, Europe, North America, Pest Control, Biological methods, Arecaceae parasitology, Genes, Insect genetics, Genetic Association Studies methods, Insect Proteins genetics, Insect Proteins isolation & purification, Smell genetics, Weevils genetics, Weevils physiology, Exome Sequencing methods
- Abstract
For decades, the American palm weevil (APW), Rhynchophorus palmarum, has been a threat to coconut and oil palm production in the Americas. It has recently spread towards North America, endangering ornamental palms, and the expanding date palm production. Its behavior presents several parallelisms with a closely related species, R. ferrugineus, the red palm weevil (RPW), which is the biggest threat to palms in Asia and Europe. For both species, semiochemicals have been used for management. However, their control is far from complete. We generated an adult antennal transcriptome from APW and annotated chemosensory related gene families to obtain a better understanding of these species' olfaction mechanism. We identified unigenes encoding 37 odorant-binding proteins (OBPs), ten chemosensory proteins (CSPs), four sensory neuron membrane proteins (SNMPs), seven gustatory receptors (GRs), 63 odorant receptors (ORs), and 28 ionotropic receptors (IRs). Noticeably, we find out the R. ferrugineus pheromone-binding protein and pheromone receptor orthologs from R. palmarum. Candidate genes identified and annotated in this study allow us to compare these palm weevils' chemosensory gene sets. Most importantly, this study provides the foundation for functional studies that could materialize as novel pest management strategies.
- Published
- 2021
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6. Odorant receptor phylogeny confirms conserved channels for sex pheromone and host plant signals in tortricid moths.
- Author
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Gonzalez F, Borrero-Echeverry F, Jósvai JK, Strandh M, Unelius CR, Tóth M, Witzgall P, Bengtsson M, and Walker WB 3rd
- Abstract
The search for mates and food is mediated by volatile chemicals. Insects sense food odorants and sex pheromones through odorant receptors (ORs) and pheromone receptors (PRs), which are expressed in olfactory sensory neurons. Molecular phylogenetics of ORs, informed by behavioral and functional data, generates sound hypotheses for the identification of semiochemicals driving olfactory behavior. Studying orthologous receptors and their ligands across taxa affords insights into the role of chemical communication in reproductive isolation and phylogenetic divergence. The female sex pheromone of green budworm moth Hedya nubiferana (Lepidoptera, Totricidae) is a blend of two unsaturated acetates, only a blend of both elicits male attraction. Females produce in addition codlemone, which is the sex pheromone of another tortricid, codling moth Cydia pomonella . Codlemone also attracts green budworm moth males. Concomitantly, green budworm and codling moth males are attracted to the host plant volatile pear ester. A congruent behavioral response to the same pheromone and plant volatile in two tortricid species suggests co-occurrence of dedicated olfactory channels. In codling moth, one PR is tuned to both compounds, the sex pheromone codlemone and the plant volatile pear ester. Our phylogenetic analysis finds that green budworm moth expresses an orthologous PR gene. Shared ancestry, and high levels of amino acid identity and sequence similarity, in codling and green budworm moth PRs offer an explanation for parallel attraction of both species to the same compounds. A conserved olfactory channel for a sex pheromone and a host plant volatile substantiates the alliance of social and habitat signals in insect chemical communication. Field attraction assays confirm that in silico investigations of ORs afford powerful predictions for an efficient identification of behavior-modifying semiochemicals, for an improved understanding of the mechanisms of host plant attraction in insect herbivores and for the further development of sustainable insect control., Competing Interests: None declared., (© 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.)
- Published
- 2020
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7. Insect chemical ecology: chemically mediated interactions and novel applications in agriculture.
- Author
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Mbaluto CM, Ayelo PM, Duffy AG, Erdei AL, Tallon AK, Xia S, Caballero-Vidal G, Spitaler U, Szelényi MO, Duarte GA, Walker WB 3rd, and Becher PG
- Abstract
Insect chemical ecology (ICE) evolved as a discipline concerned with plant-insect interactions, and also with a strong focus on intraspecific pheromone-mediated communication. Progress in this field has rendered a more complete picture of how insects exploit chemical information in their surroundings in order to survive and navigate their world successfully. Simultaneously, this progress has prompted new research questions about the evolution of insect chemosensation and related ecological adaptations, molecular mechanisms that mediate commonly observed behaviors, and the consequences of chemically mediated interactions in different ecosystems. Themed meetings, workshops, and summer schools are ideal platforms for discussing scientific advancements as well as identifying gaps and challenges within the discipline. From the 11th to the 22nd of June 2018, the 11th annual PhD course in ICE was held at the Swedish University of Agricultural Sciences (SLU) Alnarp, Sweden. The course was made up of 35 student participants from 22 nationalities (Fig. 1a) as well as 32 lecturers. Lectures and laboratory demonstrations were supported by literature seminars, and four broad research areas were covered: (1) multitrophic interactions and plant defenses, (2) chemical communication focusing on odor sensing, processing, and behavior, (3) disease vectors, and (4) applied aspects of basic ICE research in agriculture. This particular article contains a summary and brief synthesis of these main emergent themes and discussions from the ICE 2018 course. In addition, we also provide suggestions on teaching the next generation of ICE scientists, especially during unprecedented global situations., Competing Interests: Conflict of interestThe authors declare that they have no conflicts of interest., (© The Author(s) 2020.)
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- 2020
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8. A novel lineage of candidate pheromone receptors for sex communication in moths.
- Author
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Bastin-Héline L, de Fouchier A, Cao S, Koutroumpa F, Caballero-Vidal G, Robakiewicz S, Monsempes C, François MC, Ribeyre T, Maria A, Chertemps T, de Cian A, Walker WB 3rd, Wang G, Jacquin-Joly E, and Montagné N
- Subjects
- Animals, Behavior, Animal, CRISPR-Cas Systems, Drosophila genetics, Drosophila metabolism, Female, Gene Expression Regulation, Gene Knockout Techniques, Insect Proteins genetics, Lepidoptera genetics, Lepidoptera metabolism, Male, Moths genetics, Receptors, Odorant, Receptors, Pheromone classification, Receptors, Pheromone genetics, Spodoptera genetics, Spodoptera metabolism, Transcriptome, Xenopus genetics, Xenopus metabolism, Insect Proteins metabolism, Moths metabolism, Receptors, Pheromone metabolism, Sex Attractants metabolism
- Abstract
Sex pheromone receptors (PRs) are key players in chemical communication between mating partners in insects. In the highly diversified insect order Lepidoptera, male PRs tuned to female-emitted type I pheromones (which make up the vast majority of pheromones identified) form a dedicated subfamily of odorant receptors (ORs). Here, using a combination of heterologous expression and in vivo genome editing methods, we bring functional evidence that at least one moth PR does not belong to this subfamily but to a distantly related OR lineage. This PR, identified in the cotton leafworm Spodoptera littoralis , is highly expressed in male antennae and is specifically tuned to the major sex pheromone component emitted by females. Together with a comprehensive phylogenetic analysis of moth ORs, our functional data suggest two independent apparitions of PRs tuned to type I pheromones in Lepidoptera, opening up a new path for studying the evolution of moth pheromone communication., Competing Interests: LB, SC, FK, GC, SR, CM, MF, TR, AM, TC, Ad, WW, GW No competing interests declared, Ad, EJ, NM The intellectual property rights of SlitOR5 have been licensed by Inra, Sorbonne Université and CNRS for the purpose of developing novel insect control agents., (© 2019, Bastin-Héline et al.)
- Published
- 2019
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9. Transcriptome Analysis of Gene Families Involved in Chemosensory Function in Spodoptera littoralis (Lepidoptera: Noctuidae).
- Author
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Walker WB 3rd, Roy A, Anderson P, Schlyter F, Hansson BS, and Larsson MC
- Subjects
- Animals, Arthropod Antennae metabolism, Brain metabolism, Cytochrome P-450 Enzyme System genetics, Cytochrome P-450 Enzyme System metabolism, Esterases genetics, Esterases metabolism, Female, Gene Expression Profiling, Genes, Insect, Insect Proteins metabolism, Male, Multigene Family, Receptors, Cell Surface genetics, Receptors, Cell Surface metabolism, Receptors, Odorant genetics, Receptors, Odorant metabolism, Spodoptera enzymology, Spodoptera metabolism, Spodoptera physiology, Taste Perception, Insect Proteins genetics, Spodoptera genetics
- Abstract
Background: Deciphering the molecular mechanisms mediating the chemical senses, taste, and smell has been of vital importance for understanding the nature of how insects interact with their chemical environment. Several gene families are implicated in the uptake, recognition, and termination of chemical signaling, including binding proteins, chemosensory receptors and degrading enzymes. The cotton leafworm, Spodoptera littoralis, is a phytophagous pest and current focal species for insect chemical ecology and neuroethology., Results: We produced male and female Illumina-based transcriptomes from chemosensory and non-chemosensory tissues of S. littoralis, including the antennae, proboscis, brain and body carcass. We have annotated 306 gene transcripts from eight gene families with known chemosensory function, including 114 novel candidate genes. Odorant receptors responsive to floral compounds are expressed in the proboscis and may play a role in guiding proboscis probing behavior. In both males and females, expression of gene transcripts with known chemosensory function, including odorant receptors and pheromone-binding proteins, has been observed in brain tissue, suggesting internal, non-sensory function for these genes., Conclusions: A well-curated set of annotated gene transcripts with putative chemosensory function is provided. This will serve as a resource for future chemosensory and transcriptomic studies in S. littoralis and closely related species. Collectively, our results expand current understanding of the expression patterns of genes with putative chemosensory function in insect sensory and non-sensory tissues. When coupled with functional data, such as the deorphanization of odorant receptors, the gene expression data can facilitate hypothesis generation, serving as a substrate for future studies.
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- 2019
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10. A Drosophila female pheromone elicits species-specific long-range attraction via an olfactory channel with dual specificity for sex and food.
- Author
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Lebreton S, Borrero-Echeverry F, Gonzalez F, Solum M, Wallin EA, Hedenström E, Hansson BS, Gustavsson AL, Bengtsson M, Birgersson G, Walker WB 3rd, Dweck HKM, Becher PG, and Witzgall P
- Subjects
- Alkadienes metabolism, Animals, Female, Sex Attractants physiology, Species Specificity, Animal Communication, Chemotaxis, Drosophila melanogaster physiology, Olfactory Receptor Neurons physiology, Pheromones physiology, Receptors, Odorant physiology
- Abstract
Background: Mate finding and recognition in animals evolves during niche adaptation and involves social signals and habitat cues. Drosophila melanogaster and related species are known to be attracted to fermenting fruit for feeding and egg-laying, which poses the question of whether species-specific fly odours contribute to long-range premating communication., Results: We have discovered an olfactory channel in D. melanogaster with a dual affinity to sex and food odorants. Female flies release a pheromone, (Z)-4-undecenal (Z4-11Al), that elicits flight attraction in both sexes. Its biosynthetic precursor is the cuticular hydrocarbon (Z,Z)-7,11-heptacosadiene (7,11-HD), which is known to afford reproductive isolation between the sibling species D. melanogaster and D. simulans during courtship. Twin olfactory receptors, Or69aB and Or69aA, are tuned to Z4-11Al and food odorants, respectively. They are co-expressed in the same olfactory sensory neurons, and feed into a neural circuit mediating species-specific, long-range communication; however, the close relative D. simulans, which shares food resources with D. melanogaster, does not respond to Z4-11Al., Conclusion: The Or69aA and Or69aB isoforms have adopted dual olfactory traits. The underlying gene yields a collaboration between natural and sexual selection, which has the potential to drive speciation.
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- 2017
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11. Functional evolution of Lepidoptera olfactory receptors revealed by deorphanization of a moth repertoire.
- Author
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de Fouchier A, Walker WB 3rd, Montagné N, Steiner C, Binyameen M, Schlyter F, Chertemps T, Maria A, François MC, Monsempes C, Anderson P, Hansson BS, Larsson MC, and Jacquin-Joly E
- Subjects
- Action Potentials, Animals, Dose-Response Relationship, Drug, Drosophila, Female, Likelihood Functions, Male, Models, Statistical, Odorants, Phylogeny, Arthropod Antennae metabolism, Insect Proteins genetics, Olfactory Receptor Neurons metabolism, Receptors, Odorant genetics, Smell, Spodoptera physiology
- Abstract
Insects detect their hosts or mates primarily through olfaction, and olfactory receptors (ORs) are at the core of odorant detection. Each species has evolved a unique repertoire of ORs whose functional properties are expected to meet its ecological needs, though little is known about the molecular basis of olfaction outside Diptera. Here we report a pioneer functional analysis of a large array of ORs in a lepidopteran, the herbivorous pest Spodoptera littoralis. We demonstrate that most ORs are narrowly tuned to ubiquitous plant volatiles at low, relevant odorant titres. Our phylogenetic analysis highlights a basic conservation of function within the receptor repertoire of Lepidoptera, across the expansive evolutionary radiation of different major clades. Our study provides a reference for further studies of olfactory mechanisms in Lepidoptera, a historically crucial insect order in olfactory research.
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- 2017
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12. Effects of different animal manures on attraction and reproductive behaviors of common house fly, Musca domestica L.
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Shah RM, Azhar F, Shad SA, Walker WB 3rd, Azeem M, and Binyameen M
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- Animals, Cattle, Feeding Behavior, Female, Fertility, Horses, Larva, Longevity, Male, Oviposition, Reproduction, Species Specificity, Houseflies growth & development, Manure parasitology
- Abstract
Insects rely mainly on their well-developed and highly sophisticated olfactory system to discriminate volatile cues released from host and nonhost substances, mates, oviposition substrates, and food sources. Onset of first mating, mating duration, and onset of first oviposition, oviposition period, fecundity (number of eggs laid by a female), and longevity of freshly emerged Musca domestica L. (Diptera: Muscidae) adults were observed in the presence of different animal manures: cow, horse, donkey, poultry, and an artificial diet. The M. domestica adults exposed to horse manure showed a delay in onset of first mating and first oviposition, prolonged mating duration, and reduced fecundity compared to the artificial diet (control). Likewise, the fecundity was reduced in the presence of donkey manure as compared to artificial diet. The onset of first mating was delayed and duration of first mating was shortened in the presence of cow manure as compared to artificial diet and no oviposition was observed throughout the duration of the experiment. However, the reproductive behaviors and all fitness measures in adults exposed to poultry manure were similar or even better, compared to the artificial diet. Surprisingly, in a free-choice attraction assay, the highest numbers of adult flies were attracted toward the cow manure as compared to all other manures as well as the artificial diet. However, the numbers of flies captured in all other types of manures were not different than the artificial diet (control). Furthermore, chemical analysis of headspace samples of manures revealed qualitative differences in odor (volatile) profiles of all manures and artificial diet, indicating that behavioral differences could be due to the differences in the volatile chemistry of the adult ovipositional substrates and larval growth mediums. This study may contribute toward both understanding the linkage between ecological adaptations and host selection mechanisms and the development of pest management strategies against this serious pest of medical and veterinary importance.
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- 2016
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13. Data set for diet specific differential gene expression analysis in three Spodoptera moths.
- Author
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Roy A, Walker WB 3rd, Vogel H, Kushwaha SK, Chattington S, Larsson MC, Anderson P, Heckel DG, and Schlyter F
- Abstract
Examination of closely related species pairs is suggested for evolutionary comparisons of different degrees of polyphagy, which we did here with three taxa of lepidopteran herbivores, Spodoptera spp (S. littoralis, S. frugiperda maize (C) and rice (R) strains) for a RNAseq analysis of the midguts from the 3rd instar insect larvae for differential metabolic responses after feeding on pinto bean based artificial diet vs maize leaves. Paired-end (2×100 bp) Illumina HiSeq2500 sequencing resulted in a total of 24, 23, 24, and 21 million reads for the SF-C-Maize, SF-C-Pinto, SF-R-Maize, SF-R Pinto, and a total of 35 and 36 million reads for the SL-Maize and SL-Pinto samples, respectively. After quality control measures, a total of 62.2 million reads from SL and 71.7 million reads from SF were used for transcriptome assembly (TA). The resulting final de novo reference TA (backbone) for the SF taxa contained 37,985 contigs with a N50 contig size of 1030 bp and a maximum contig length of 17,093 bp, while for SL, 28,329 contigs were generated with a N50 contig size of 1980 bp and a maximum contig length of 18,267 bp. The data presented herein contains supporting information related to our research article Roy et al. (2016) http://dx.doi.org/10.1016/j.ibmb.2016.02.006[1].
- Published
- 2016
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14. Diet dependent metabolic responses in three generalist insect herbivores Spodoptera spp.
- Author
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Roy A, Walker WB 3rd, Vogel H, Chattington S, Larsson MC, Anderson P, Heckel DG, and Schlyter F
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- Animals, Feeding Behavior, Herbivory genetics, Homeodomain Proteins, Host Specificity, Plant Leaves parasitology, Spodoptera genetics, Tumor Suppressor Proteins, Animal Feed analysis, Herbivory physiology, Plants parasitology, Spodoptera physiology
- Abstract
Adaption to dietary changes is critical in the evolution of host plant ranges in polyphagous insects. We compared three taxa of lepidopteran herbivores from the predominantly generalist genus Spodoptera showing different degrees of polyphagy: Spodoptera littoralis, with a broad host range including both mono- and dicotyledonous plants, and two Spodoptera frugiperda strains [Corn (i.e. maize) (C) and Rice (R)] adapted primarily to different grass species. When feeding on maize we show a lower performance in the broad generalist taxon compared to the grass adapted taxa. Among these taxa, the maize adapted S. frugiperda C-strain generally performed better than the R-strain on maize leaves. On artificial pinto diet, all taxa performed well. Our RNA-Seq analysis of midgut transcriptomes from 3rd instar larvae feeding on maize showed broader transcriptional readjustments in the generalist S. littoralis compared to grass adapted S. frugiperda strains. Substantial alteration in the expression levels of midgut physiological function related transcripts, such as digestive and detoxifying enzymes, transporters, immunity, and peritrophic membrane associated transcripts, existed in all taxa. We found high background expression of UDP-glucosyl transferases, which are known to neutralize maize leaf toxins, in the maize adapted S. frugiperda C-strain, contributing to its fitness on maize compared to the R-strain. Our findings provide evidence for divergent diet specific response of digestive physiology within these Spodoptera taxa. Unexpectedly, the C- and R-strains of S. frugiperda fed on the same diet showed large differences in expression patterns between these two closely related taxa., (Copyright © 2016. Published by Elsevier Ltd.)
- Published
- 2016
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15. The chemosensory receptors of codling moth Cydia pomonella-expression in larvae and adults.
- Author
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Walker WB 3rd, Gonzalez F, Garczynski SF, and Witzgall P
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- Animals, Animals, Newborn, Female, Gene Expression Regulation, Developmental, Larva genetics, Male, Moths growth & development, Phylogeny, Sequence Homology, Nucleic Acid, Sexism, Gene Expression Profiling methods, Insect Proteins genetics, Moths genetics, Receptors, Odorant genetics, Sequence Analysis, RNA methods
- Abstract
Olfaction and gustation play critical roles in the life history of insects, mediating vital behaviors such as food, mate and host seeking. Chemosensory receptor proteins, including odorant receptors (ORs), gustatory receptors (GRs) and ionotropic receptors (IRs) function to interface the insect with its chemical environment. Codling moth, Cydia pomonella, is a worldwide pest of apple, pear and walnut, and behavior-modifying semiochemicals are used for environmentally safe control. We produced an Illumina-based transcriptome from antennae of males and females as well as neonate head tissue, affording a qualitative and quantitative analysis of the codling moth chemosensory receptor repertoire. We identified 58 ORs, 20 GRs and 21 IRs, and provide a revised nomenclature that is consistent with homologous sequences in related species. Importantly, we have identified several OR transcripts displaying sex-biased expression in adults, as well as larval-enriched transcripts. Our analyses have expanded annotations of the chemosensory receptor gene families, and provide first-time transcript abundance estimates for codling moth. The results presented here provide a strong foundation for future work on codling moth behavioral physiology and ecology at the molecular level, and may lead to the development of more precise biorational control strategies.
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- 2016
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16. Saliva of Lygus lineolaris digests double stranded ribonucleic acids.
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Allen ML and Walker WB 3rd
- Subjects
- Animals, DNA, Female, Gene Knockdown Techniques, Hot Temperature, Inhibitor of Apoptosis Proteins, Male, Ribonuclease III, Hemiptera metabolism, Hemolymph metabolism, RNA, Double-Stranded metabolism, Saliva metabolism, Salivary Glands metabolism
- Abstract
The prospects for development of highly specific pesticides based on double stranded ribonucleic acid have been a recent focus of scientific research. Creative applications have been proposed and demonstrated. However, not all insects are sensitive to double stranded RNA (dsRNA) gene knockdown effects; applications in the order Lepidoptera, for example, have met with varied success. Gene knockdown has been demonstrated in several species in the order Hemiptera. In our laboratory, knockdown experiments relied on microinjection of dsRNA into the hemocoel of the tarnished plant bug, Lygus lineolaris. Subsequent experiments delivering dsRNA to insects by feeding were repeatedly unsuccessful in demonstrating knockdown, and a hypothesis was formulated that the dsRNA was digested and degraded by the insect prior to contact with the insect cells. Exposure of dsRNA to insect saliva, insect salivary glands, and insect hemolymph was compared with commercial RNAase III. The saliva of L. lineolaris was found to rapidly digest double stranded RNA. RNAase inhibitor did not affect the activity but heat treatment slowed enzymatic activity., (Published by Elsevier Ltd.)
- Published
- 2012
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17. Candidate chemosensory genes in female antennae of the noctuid moth Spodoptera littoralis.
- Author
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Jacquin-Joly E, Legeai F, Montagné N, Monsempes C, François MC, Poulain J, Gavory F, Walker WB 3rd, Hansson BS, and Larsson MC
- Subjects
- Animals, Expressed Sequence Tags, Female, Male, Arthropod Antennae metabolism, Insect Proteins metabolism, Spodoptera metabolism
- Abstract
Chemical senses are crucial for all organisms to detect various environmental information. Different protein families, expressed in chemosensory organs, are involved in the detection of this information, such as odorant-binding proteins, olfactory and gustatory receptors, and ionotropic receptors. We recently reported an Expressed Sequence Tag (EST) approach on male antennae of the noctuid moth, Spodoptera littoralis, with which we could identify a large array of chemosensory genes in a species for which no genomic data are available.Here we describe a complementary EST project on female antennae in the same species. 18,342 ESTs were sequenced and their assembly with our previous male ESTs led to a total of 13,685 unigenes, greatly improving our description of the S. littoralis antennal transcriptome. Gene ontology comparison between male and female data suggested a similar complexity of antennae of both sexes. Focusing on chemosensation, we identified 26 odorant-binding proteins, 36 olfactory and 5 gustatory receptors, expressed in the antennae of S. littoralis. One of the newly identified gustatory receptors appeared as female-enriched. Together with its atypical tissue-distribution, this suggests a role in oviposition. The compilation of male and female antennal ESTs represents a valuable resource for exploring the mechanisms of olfaction in S. littoralis.
- Published
- 2012
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