47 results on '"Wakatsuki H"'
Search Results
2. Laser irradiation induced disintegration of a bubble in a glass melt
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Juodkazis, S., primary, Murazawa, N., additional, Wakatsuki, H., additional, and Misawa, H., additional
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- 2007
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3. Importance of Debye and Keesom Interactions in Separating m-Xylene and p-Xylene in GC-MS Analysis Utilizing PEG Stationary Phase
- Author
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Kanai, H., primary, Inouye, V., additional, Yazawa, L., additional, Goo, R., additional, and Wakatsuki, H., additional
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- 2005
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4. Photodegradation kinetics of fenitrothion in various aqueous media and its effect on steroid hormones biosynthesis
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Derbalah, A. S. H., primary, Wakatsuki, H., additional, Yamazaki, T., additional, and Sakugawa, H., additional
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- 2004
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5. DIFFERENTIAL ROLES OF NO AND CO IN NEOCORTICAL LONG-TERM POTENTIATION IN LAYER V
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WAKATSUKI, H., primary, KUDOH, M., additional, and SHIBUKI, K., additional
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- 1997
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6. DIFFERENTIAL ROLES OF NO AND CO IN CEREBELLAR LONG-TERM DEPRESSION
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SHIBUKI, K., primary, KIMURA, S., additional, and WAKATSUKI, H., additional
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- 1997
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7. Interply Shear Stresses and Coupled Deformations of a Folded Belt Structure Under Extension
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Akasaka, T., primary, Kato, M., additional, and Wakatsuki, H., additional
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- 1992
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8. Hypodipsic Hypernatremia Associated with Absence of Septum Lucidum and Olfactory Dysfunction.
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OHZEKI, T., HANAKI, K., ASANO, T., ISHITANI, N., WAKATSUKI, H., and SHIRAKI, K.
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- 1986
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9. Stocastic Measurement of Signals in Flame Atomic Absorption Spectrophotometry.
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Kanai, H., Goo, R., and Wakatsuki, H.
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- 1978
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10. Irreversible impairment of inhibitory neurons and nitric oxide release in the neocortex produced by low temperature and hypoxia in vitro
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Fujisaki, T., Wakatsuki, H., Kudoh, M., and Shibuki, K.
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- 1999
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11. Metal powder addition effect on the photocatalytic reactions and the photo-generated electric charge collected at an inert electrode in aqueous TiO~2 suspensions
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Hirano, K., Asayama, H., Hoshino, A., and Wakatsuki, H.
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- 1997
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12. Guide for Troubleshooting Operator Problems in the Hall 700 and 700A Electrolytic Conductivity Detectors
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Inouye, V. Y., primary, Kanai, H., additional, Goo, R., additional, and Wakatsuki, H., additional
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- 1984
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13. Stocastic variability of noise with the Hall electrolytic conductivity detector for gas chromatography
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Goo, R. K. S., primary, Kanai, H., additional, Inouye, V., additional, and Wakatsuki, H., additional
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- 1980
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14. Kanzo
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Ohta, G, primary, Kajihara, M, additional, and Wakatsuki, H, additional
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- 1969
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15. Press-formed aspherical micro lens made of bismuth based glass with high refractive index and transparency.
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Sekine, M., Sugimoto, N., and Wakatsuki, H.
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- 2004
16. P-66 - DIFFERENTIAL ROLES OF NO AND CO IN NEOCORTICAL LONG-TERM POTENTIATION IN LAYER V
- Author
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WAKATSUKI, H., KUDOH, M., and SHIBUKI, K.
- Published
- 1997
- Full Text
- View/download PDF
17. P-65 - DIFFERENTIAL ROLES OF NO AND CO IN CEREBELLAR LONG-TERM DEPRESSION
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SHIBUKI, K., KIMURA, S., and WAKATSUKI, H.
- Published
- 1997
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- View/download PDF
18. Enhanced decreases in rice evapotranspiration in response to elevated atmospheric carbon dioxide under warmer environments.
- Author
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Ikawa H, Hasegawa T, Kumagai E, Wakatsuki H, Sekiyama Y, Nagano AJ, and Kuwagata T
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- Temperature, Vapor Pressure, Plant Stomata physiology, Plant Stomata drug effects, Models, Biological, Atmosphere chemistry, Hot Temperature, Oryza physiology, Oryza metabolism, Carbon Dioxide metabolism, Carbon Dioxide pharmacology, Plant Transpiration physiology
- Abstract
A short period of exposure to elevated CO
2 is known to decrease evapotranspiration via stomatal closure. Based on theoretical evaluation of a canopy transpiration model, we hypothesized that this decrease in the evapotranspiration of rice under elevated CO2 was greater under higher temperature conditions due to an increased sensitivity of transpiration to changes in CO2 induced by the greater vapour pressure deficit. In a temperature gradient chamber-based experiment, a 200 ppm increase in CO2 concentration led to 0.4 mm (-7%) and 1.5 mm (-15%) decreases in 12 h evapotranspiration under ambient temperature and high temperature (+3.7°C) conditions, respectively. Model simulations revealed that the greater vapour pressure deficit under higher temperature conditions explained the variations in the reduction of evapotranspiration observed under elevated CO2 levels between the temperature treatments. Our study suggests the utility of a simple modelling framework for mechanistic understanding of evapotranspiration and crop energy balance system under changing environmental conditions., (© 2024 John Wiley & Sons Ltd.)- Published
- 2024
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19. A dataset for analyzing the climate change response of grain quality of 48 Japanese rice cultivars with contrasting levels of heat tolerance.
- Author
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Wakatsuki H, Takimoto T, Ishigooka Y, Nishimori M, Sakata M, Saida N, Akagi K, Makowski D, and Hasegawa T
- Abstract
Climate change has a significant impact on rice grain appearance quality; in particular, high temperatures during the grain filling period increase the rate of chalky immature grains, reducing the marketability of rice. Heat-tolerant cultivars have been bred and released to reduce the rate of chalky grain and improve rice quality under high temperatures, but the ability of these cultivars to actually reduce chalky grain content has never been demonstrated due to the lack of integrated datasets. Here, we present a dataset collected through a systematic literature search from publicly available data sources, for the quantitative analysis of the impact of meteorological factors on grain appearance quality of various rice cultivars with contrasted heat tolerance levels. The dataset contains 1302 field observations of chalky grain rates (%) - a critical trait affecting grain appearance sensitive to temperature shocks - for 48 cultivars covering five different heat-tolerant ranks (HTRs) collected at 44 sites across Japan. The dataset also includes the values of key meteorological variables during the grain filling period, such as the cumulative mean air temperature above the threshold temperature (TaHD), mean solar radiation, and mean relative humidity over 20 days after heading, obtained from a gridded daily meteorological dataset with a 1-km resolution developed by the National Agriculture and Food Research Organization. The dataset covers major commercial rice cultivars cultivated in Japan in different environmental conditions. It is a useful resource for analyzing the climate change impact on crop quality and assess the effectiveness of genetic improvements in heat tolerance. Its value has been illustrated in the research article entitled "Effectiveness of heat tolerance rice cultivars in preserving grain appearance quality under high temperatures - A meta-analysis", where the dataset was used to develop a statistical model quantifying the effects of high temperature on grain quality as a function of cultivar heat tolerance., (© 2024 The Author(s).)
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- 2024
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20. Assessment of anthracycline-induced cardiotoxicity in childhood cancer survivors during long-term follow-up using strain analysis and intraventricular pressure gradient measurements.
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Hosono Y, Takahashi K, Shigemitsu S, Akimoto S, Ifuku M, Yazaki K, Wakatsuki H, Yaguchi A, Tomita O, Fujimura J, Saito M, Yoneoka D, and Shimizu T
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- Humans, Child, Adolescent, Young Adult, Adult, Child, Preschool, Cardiotoxicity drug therapy, Anthracyclines adverse effects, Ventricular Pressure, Follow-Up Studies, Antibiotics, Antineoplastic adverse effects, Cancer Survivors, Neoplasms drug therapy, Neoplasms complications, Heart Diseases diagnosis, Heart Diseases diagnostic imaging
- Abstract
Background: Cardiac dysfunction due to cardiotoxicity from anthracycline chemotherapy is a leading cause of morbidity and mortality in childhood cancer survivors (CCS), and the cumulative incidence of cardiac events has continued to increase. This study identifies an adequate indicator of cardiac dysfunction during long-term follow-up., Procedure: In total, 116 patients (median age: 15.5 [range: 4.7-40.2] years) with childhood cancer who were treated with anthracycline were divided into three age groups for analysis (C1: 4-12 years of age, C2: 13-18 years of age, C3: 19-40 years of age), and 116 control patients of similar ages were divided into three corresponding groups (N1, N2, and N3). Layer-specific strains were assessed for longitudinal strain (LS) and circumferential strain (CS). The total and segmental intraventricular pressure gradients (IVPG) were also calculated based on Doppler imaging of the mitral inflow using Euler's equation., Results: Conventional echocardiographic parameters were not significantly different between the patients and controls. All layers of the LS and inner and middle layers of the basal and papillary CS in all ages and all IVPGs in C2 and C3 decreased compared to those of corresponding age groups. Interestingly, basal CS and basal IVPG in CCS showed moderate correlation and both tended to rapidly decrease with aging. Furthermore, basal IVPG and anthracycline dose showed significant correlations., Conclusions: Basal CS and total and basal IVPGs may be particularly useful indicators of cardiotoxicity in long-term follow-up., (© 2023. Springer Nature Japan KK, part of Springer Nature.)
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- 2024
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21. Free Flap Survival with External Jugular Vein as a Detour Due to Internal Jugular Vein Thrombosis.
- Author
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Sotsuka Y, Murakami A, Wakatsuki H, and Matsuda K
- Abstract
In head and neck reconstructions, venous anastomosis of the free flap to the internal jugular vein system is a common procedure. However, in some cases, postoperative complications such as internal jugular vein thrombosis can occur. In this report, we present a case where postoperative internal jugular vein thrombosis was identified after venous anastomosis of the free flap to the internal jugular vein system. In this case, preserving the external jugular vein allowed for retrograde flow of venous blood in the internal jugular vein to enter the external jugular vein, serving as an alternative drainage pathway. This finding highlights the potential benefits of preserving the external jugular vein in head and neck surgery involving free flap venous anastomosis to mitigate the adverse effects of internal jugular vein thrombosis. Further investigations are warranted to better understand the underlying mechanisms and optimize surgical approaches for improved patient outcomes., Competing Interests: The authors have no financial interest to declare in relation to the content of this article., (Copyright © 2023 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons.)
- Published
- 2023
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22. Perilla seed oil improves bone health by inhibiting bone resorption in healthy Japanese adults: A 12-month, randomized, double-blind, placebo-controlled trial.
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Matsuzaki K, Hossain S, Wakatsuki H, Tanabe Y, Ohno M, Kato S, Shido O, and Hashimoto M
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- Humans, Middle Aged, Tartrate-Resistant Acid Phosphatase, East Asian People, Plant Oils pharmacology, Plant Oils therapeutic use, Biomarkers, Bone Density, Bone Resorption drug therapy
- Abstract
Accumulating evidence suggests the beneficial effect of omega-3 polyunsaturated fatty acids (PUFAs) on bone mineral density (BMD). However, the effects of perilla (Perilla frutescens) seed oil (PO), a rich source of α-linoleic acid (LNA), on human bone have not yet been elucidated. This randomized, double-blind, placebo-controlled trial investigated the effects of long-term PO intake on bone health in Japanese adults. After screening for eligibility, 52 participants (mean age 54.2 ± 6.4 years) were randomly assigned to placebo (n = 25) and PO (n = 27) groups, which received 7.0 ml of olive oil and PO daily, respectively. At baseline and 12-month, quantitative ultrasound of the right calcaneus was measured with an ultrasound bone densitometer and percentage of the Young Adult Mean (%YAM) was calculated. Serum levels of tartrate-resistant acid phosphatase 5b (TRACP-5b), and bone alkaline phosphatase (BALP) were evaluated. In addition, PUFA levels in the erythrocyte plasma membrane (RBC-PM), serum biological antioxidant potential (BAP), and diacron reactive oxygen metabolites (d-ROM) were evaluated. Compared with the placebo group, %YAM levels increased and serum TRACP-5b levels decreased significantly in the PO group at 12-month, while serum BALP levels remained unchanged. Moreover, RBC-PM LNA levels and BAP/d-ROM ratios increased significantly in the PO compared with the placebo group. These results suggest that long-term PO intake may improve age-related BMD decline by suppressing bone resorption and increasing LNA levels., (© 2023 John Wiley & Sons Ltd.)
- Published
- 2023
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23. Perilla frutescens seed oil combined with Anredera cordifolia leaf powder attenuates age-related cognitive decline by reducing serum triglyceride and glucose levels in healthy elderly Japanese individuals: a possible supplement for brain health.
- Author
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Hashimoto M, Matsuzaki K, Maruyama K, Sumiyoshi E, Hossain S, Wakatsuki H, Kato S, Ohno M, Tanabe Y, Kuroda Y, Yamaguchi S, Kajima K, Ohizumi Y, and Shido O
- Subjects
- Aged, Animals, Brain metabolism, Dietary Supplements, Glucose metabolism, Humans, Japan, Mice, Plant Leaves metabolism, Plant Oils metabolism, Powders metabolism, Triglycerides metabolism, Cognitive Dysfunction drug therapy, Perilla frutescens metabolism
- Abstract
We have shown that Anredera cordifolia extract improves learning and memory in a senescence-accelerated mouse model, and that α-linolenic acid (ALA)-rich Perilla frutescens seed oil (PO) improves brain function in healthy Japanese adults and elderly individuals. Herein, we present a 12-month, randomised, double-blind, parallel-armed intervention trial examining the effects of PO supplementation alone or in combination with A. cordifolia leaf powder on brain function in healthy elderly Japanese individuals. Participants were randomly divided into two groups: the PO group received 1.47 mL PO (0.88 g ALA) daily via soft gelatine capsules, and the POAC group received 1.47 mL PO and 1.12 g A. cordifolia leaf powder (1.46 mg vitexin and 1.12 mg adenosine) daily. After 12 months of intervention, the POAC group showed generally higher cognitive index scores than the PO group. The beneficial effects of combined supplementation on cognitive function were associated with increased ALA and eicosapentaenoic acid levels in red blood cell plasma membranes, increased serum biological antioxidant potential, and decreased serum triglyceride, glucose, and N -(epsilon)-carboxymethyl-lysine (CML), an advanced glycation end-product and biochemical marker of oxidative stress levels. The effects of combined supplementation on cognitive function also showed a significant negative correlation with serum CML levels after 12 months of intervention. Our findings suggest that combined long-term supplementation with PO and A. cordifolia more effectively ameliorates age-related cognitive decline than PO alone. These findings may serve as a basis for the development of new supplements for brain health. Clinical Trial Registry, UMIN000040863.
- Published
- 2022
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24. Schlieren Phenomenon for Identification of Lymphorrhea Point.
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Sotsuka Y, Wakatsuki H, and Matsuda K
- Abstract
Surgical invasion of the lymphatic system can lead to lymphorrhea. Lymphorrhea is first treated conservatively, but is often refractory and subsequently treated surgically. In surgery, it is difficult to identify the lymphatic leak points visually. In this study, we observed the schlieren phenomenon based on the difference in the refractive index between glucose solution and lymph fluid, and were able to easily identify the site of the lymphatic leakage in real time and treat lymphorrhea., (Copyright © 2022 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons.)
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- 2022
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25. Perilla seed oil in combination with nobiletin-rich ponkan powder enhances cognitive function in healthy elderly Japanese individuals: a possible supplement for brain health in the elderly.
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Hashimoto M, Matsuzaki K, Maruyama K, Hossain S, Sumiyoshi E, Wakatsuki H, Kato S, Ohno M, Tanabe Y, Kuroda Y, Yamaguchi S, Kajima K, Ohizumi Y, and Shido O
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- Aged, Aged, 80 and over, Antioxidants administration & dosage, Antioxidants chemistry, Double-Blind Method, Fatty Acids, Omega-3 metabolism, Female, Flavones administration & dosage, Flavones chemistry, Humans, Male, Plant Oils administration & dosage, Plant Oils chemistry, Plant Oils pharmacology, Treatment Outcome, alpha-Linolenic Acid administration & dosage, alpha-Linolenic Acid chemistry, Antioxidants pharmacology, Cognition drug effects, Cognitive Dysfunction prevention & control, Dietary Supplements, Flavones pharmacology, Perilla frutescens, alpha-Linolenic Acid pharmacology
- Abstract
Perilla ( Perilla frutescens ) seed oil (PO), rich in α-linolenic acid (ALA), can improve cognitive function in healthy elderly Japanese people. Here, supplements containing either PO alone or PO with nobiletin-rich air-dried immature ponkan powder were examined for their effects on cognitive function in 49 healthy elderly Japanese individuals. Patients were enrolled in a 12-month randomized, double-blind, parallel-armed study. Randomized participants in the PO group received soft gelatin capsules containing 1.47 mL (0.88 g of ALA) of PO daily, and those in the PO + ponkan powder (POPP) group received soft gelatin capsules containing both 1.47 mL of PO and 1.12 g ponkan powder (2.91 mg of nobiletin) daily. At the end of intervention, the POPP group showed significantly higher cognitive index scores than the PO group. The pro-cognitive effects of POPP treatment were accompanied by increases in ALA and docosahexaenoic acid levels in red blood cell plasma membranes, serum brain-derived neurotropic factor (BDNF) levels, and biological antioxidant potential. We demonstrate that 12-month intervention with POPP enhances serum BDNF and antioxidant potential, and may improve age-related cognitive impairment in healthy elderly people by increasing red blood cell ω-3 fatty acid levels. Clinical Trial Registry, UMIN000040863.
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- 2022
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26. A global dataset for the projected impacts of climate change on four major crops.
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Hasegawa T, Wakatsuki H, Ju H, Vyas S, Nelson GC, Farrell A, Deryng D, Meza F, and Makowski D
- Abstract
Reliable estimates of the impacts of climate change on crop production are critical for assessing the sustainability of food systems. Global, regional, and site-specific crop simulation studies have been conducted for nearly four decades, representing valuable sources of information for climate change impact assessments. However, the wealth of data produced by these studies has not been made publicly available. Here, we develop a global dataset by consolidating previously published meta-analyses and data collected through a new literature search covering recent crop simulations. The new global dataset builds on 8703 simulations from 202 studies published between 1984 and 2020. It contains projected yields of four major crops (maize, rice, soybean, and wheat) in 91 countries under major emission scenarios for the 21st century, with and without adaptation measures, along with geographical coordinates, current temperature and precipitation levels, projected temperature and precipitation changes. This dataset provides a solid basis for a quantitative assessment of the impacts of climate change on crop production and will facilitate the rapidly developing data-driven machine learning applications., (© 2022. The Author(s).)
- Published
- 2022
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27. Intake of Docosahexaenoic Acid-Enriched Milk Beverage Prevents Age-Related Cognitive Decline and Decreases Serum Bone Resorption Marker Levels.
- Author
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Ichinose T, Matsuzaki K, Kato M, Tanabe Y, Tachibana N, Morikawa M, Kato S, Ohata S, Ohno M, Wakatsuki H, Hossain S, Shido O, and Hashimoto M
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- Aged, Animals, Asian People, Biomarkers blood, Dementia etiology, Docosahexaenoic Acids blood, Double-Blind Method, Eicosapentaenoic Acid administration & dosage, Erythrocyte Membrane metabolism, Female, Humans, Male, Middle Aged, Alkaline Phosphatase blood, Bone Resorption diagnosis, Bone Resorption prevention & control, Cognitive Aging, Cognitive Dysfunction etiology, Cognitive Dysfunction prevention & control, Dementia prevention & control, Dietary Supplements, Docosahexaenoic Acids administration & dosage, Eating physiology, Milk, Tartrate-Resistant Acid Phosphatase blood
- Abstract
The pathogenic mechanism of dementia is still unknown, and the fundamental treatment remains to be established. Thus, there is growing interest in preventing dementia through diet. One of the functional ingredients attracting attention is docosahexaenoic acid. We conducted a 12-month, randomized, double-blind, placebo-controlled clinical trial in healthy elderly Japanese individuals with a Mini-Mental State Examination score of 28 or higher at baseline using a docosahexaenoic acid-enriched milk beverage containing 297 mg docosahexaenoic acid and 137 mg eicosapentaenoic acid. Consumption of a docosahexaenoic acid-enriched milk beverage increased the fatty acid levels of docosahexaenoic acid and eicosapentaenoic acid in erythrocyte membranes, which was the primary outcome of this study. Moreover, intake of this beverage prevented age-related cognitive decline and decreased serum bone resorption marker levels. Our data demonstrate that, even at a low dose, long-term daily intake of docosahexaenoic acid prevents dementia and may show beneficial effect on bone health.
- Published
- 2021
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28. Perilla Seed Oil Enhances Cognitive Function and Mental Health in Healthy Elderly Japanese Individuals by Enhancing the Biological Antioxidant Potential.
- Author
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Hashimoto M, Matsuzaki K, Hossain S, Ito T, Wakatsuki H, Tanabe Y, Ohno M, Kato S, Yamashita K, and Shido O
- Abstract
Oxidative stress plays an important role in age-associated cognitive decline. We recently reported that dietary intake of perilla seed oil (PO), a rich source of α-linolenic acid (LNA, C18:3, ω-3), helps in maintaining good mental health in adults. This study aimed to investigate the impacts of dietary PO intake on cognitive functions and mental health in healthy, elderly Japanese individuals. Seventy-five healthy volunteers aged 64-84 years were randomly divided into two groups: a control group and a PO-administered group. At baseline and at 12 months of intervention, cognitive function, mental health condition, fatty acid profile of the red blood cell plasma membranes (RBC-PM), and serum biochemical parameters were evaluated. Results showed that serum biological antioxidant potential and LNA levels in the RBC-PM at 12 months after the trial were significantly higher in the PO group compared to the control group. Further, both the cognitive function measures, as evaluated by the Frontal Assessment Battery test and the apathy scores, tended to be improved after 12 months in the PO group. Our results demonstrate that dietary PO intake enhances the antioxidant potential and prevents the age-related cognitive and mental decline in healthy elderly individuals by enhancing the blood LNA levels.
- Published
- 2021
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29. β-adrenergic receptor signaling evokes the PKA-ASK axis in mature brown adipocytes.
- Author
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Hattori K, Wakatsuki H, Sakauchi C, Furutani S, Sugawara S, Hatta T, Natsume T, and Ichijo H
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- Animals, Energy Metabolism, Enzyme Activation, Gene Expression Regulation, HEK293 Cells, Humans, MAP Kinase Kinase Kinase 5 genetics, MAP Kinase Kinase Kinases genetics, MAP Kinase Signaling System, Male, Mice, Phosphorylation, Serine metabolism, Threonine metabolism, Adipocytes, Brown metabolism, Cyclic AMP-Dependent Protein Kinases metabolism, MAP Kinase Kinase Kinase 5 metabolism, MAP Kinase Kinase Kinases metabolism, Receptors, Adrenergic, beta metabolism
- Abstract
Boosting energy expenditure by harnessing the activity of brown adipocytes is a promising strategy for combatting the global epidemic of obesity. Many studies have revealed that the β3-adrenergic receptor agonist is a potent activator of brown adipocytes, even in humans, and PKA and p38 MAPK have been demonstrated for regulating the transcription of a wide range of critical genes such as Ucp1. We previously revealed that the PKA-ASK1-p38 axis is activated in immature brown adipocytes and contributes to functional maturation. However, the downstream mechanisms of PKA that initiate the p38 MAPK cascade are still mostly unknown in mature brown adipocytes. Here, we identified the ASK family as a crucial signaling molecule bridging PKA and MAPK in mature brown adipocytes. Mechanistically, the phosphorylation of ASK1 at threonine 99 and serine 993 is critical in PKA-dependent ASK1 activation. Additionally, PKA also activates ASK2, which contributes to MAPK regulation. These lines of evidence provide new details for tailoring a βAR-dependent brown adipocyte activation strategy., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2020
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30. Predicting biomass of rice with intermediate traits: Modeling method combining crop growth models and genomic prediction models.
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Toda Y, Wakatsuki H, Aoike T, Kajiya-Kanegae H, Yamasaki M, Yoshioka T, Ebana K, Hayashi T, Nakagawa H, Hasegawa T, and Iwata H
- Subjects
- Genome, Plant, Genomics methods, Genotype, Machine Learning, Phenotype, Plant Breeding, Biomass, Models, Genetic, Oryza genetics, Oryza growth & development
- Abstract
Genomic prediction (GP) is expected to become a powerful technology for accelerating the genetic improvement of complex crop traits. Several GP models have been proposed to enhance their applications in plant breeding, including environmental effects and genotype-by-environment interactions (G×E). In this study, we proposed a two-step model for plant biomass prediction wherein environmental information and growth-related traits were considered. First, the growth-related traits were predicted by GP. Second, the biomass was predicted from the GP-predicted values and environmental data using machine learning or crop growth modeling. We applied the model to a 2-year-old field trial dataset of recombinant inbred lines of japonica rice and evaluated the prediction accuracy with training and testing data by cross-validation performed over two years. Therefore, the proposed model achieved an equivalent or a higher correlation between the observed and predicted values (0.53 and 0.65 for each year, respectively) than the model in which biomass was directly predicted by GP (0.40 and 0.65 for each year, respectively). This result indicated that including growth-related traits enhanced accuracy of biomass prediction. Our findings are expected to contribute to the spread of the use of GP in crop breeding by enabling more precise prediction of environmental effects on crop traits., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2020
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31. Efficacy of gelatin hydrogels incorporating triamcinolone acetonide for prevention of fibrosis in a mouse model.
- Author
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Nakajima N, Hashimoto S, Sato H, Takahashi K, Nagoya T, Kamimura K, Tsuchiya A, Yokoyama J, Sato Y, Wakatsuki H, Miyata M, Akashi Y, Tanaka R, Matsuda K, Tabata Y, and Terai S
- Abstract
Introduction: Triamcinolone acetonide (TA), a steroid, is often used clinically to prevent dysfunctions associated with fibrosis. The objective of this study was to examine whether TA can be suspended in a gelatin sheet for tissue engineering using a mouse skin wound model., Methods: TA was suspended in biodegradable gelatin and freeze-dried in a sheet form. The sheet was analyzed for homogeneity and controlled release of TA by high-performance liquid chromatography. We made two skin wounds on the dorsal side of mice. Gelatin sheets with TA (TA sheet) and without TA (control sheet) were attached to each skin wound. To determine the efficacy of the prepared TA sheet on the skin wounds, TA-sheet versus TA-injection experiments were conducted. Hematoxylin and eosin staining was performed to assess the grade of epithelialization and alpha smooth muscle actin (α-SMA) immunohistochemical staining was conducted to evaluate myofibroblast infiltration., Results: In the TA-release test in vitro , 7.7 ± 2.3% of TA was released from the sheet by 24 h. After replacing the initial phosphate-buffered saline (PBS) with collagenase PBS, the amount of released TA increased over time. The wound area/original skin wound area after 15 days with the TA sheet was significantly larger than that with the control sheet (26.9 ± 5.5% vs 10.7 ± 2.6%, p = 0.023). The α-SMA positive area/whole area with the TA sheet was significantly lower than that with the control sheet (4.65 ± 0.66% vs 7.24 ± 0.7%, p = 0.023). Furthermore, the α-SMA positive area/whole area with the TA sheet was significantly lower than that with TA injection (5.32 ± 0.45% vs 7.93 ± 0.75%, p = 0.013)., Conclusions: We developed a TA sheet and confirmed both the homogeneity of the suspended TA and controlled-release of the TA in the presence of collagenase in vitro . The TA sheet caused less myofibroblast infiltration into the tissue than the control sheet or TA injection did.
- Published
- 2019
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32. A High-Yielding Rice Cultivar "Takanari" Shows No N Constraints on CO 2 Fertilization.
- Author
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Hasegawa T, Sakai H, Tokida T, Usui Y, Nakamura H, Wakatsuki H, Chen CP, Ikawa H, Zhang G, Nakano H, Matsushima MY, and Hayashi K
- Abstract
Enhancing crop yield response to elevated CO
2 concentrations (E-[CO2 ]) is an important adaptation measure to climate change. A high-yielding indica rice cultivar "Takanari" has recently been identified as a potential candidate for high productivity in E-[CO2 ] resulting from its large sink and source capacities. To fully utilize these traits, nitrogen should play a major role, but it is unknown how N levels influence the yield response of Takanari to E-[CO2 ]. We therefore compared grain yield and quality of Takanari with those of Koshihikari, a standard japonica cultivar, in response to Free-Air CO2 enrichment (FACE, +200 μmol mol-1 ) under three N levels (0, 8, and 12 g m-2 ) over three seasons. The biomass of both cultivars increased under E-[CO2 ] at all N levels; however, the harvest index decreased under E-[CO2 ] in the N-limited treatment for Koshihikari but not for Takanari. The decreased harvest index of Koshihikari resulted from limited enhancement of spikelet number under N-limitation. In contrast, spikelet number increased in E-[CO2 ] in Takanari even without N application, resulting in significant yield enhancement, averaging 18% over 3 years, whereas Koshihikari exhibited virtually no increase in yield in E-[CO2 ] under the N-limited condition. Grain appearance quality of Koshihikari was severely reduced by E-[CO2 ], most notably in N-limited and hot conditions, by a substantial increase in chalky grain, but chalky grain % did not increase in E-[CO2 ] even without N fertilizer. These results indicated that Takanari could retain its high yield advantage over Koshihikari with limited increase in chalkiness even under limited N conditions and that it could be a useful genetic resource for improving N use efficiency under E-[CO2 ].- Published
- 2019
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33. Development of a mouse nerve-transfer model for brachial plexus injury.
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Wakatsuki H, Shibata M, Matsuda K, and Sato N
- Subjects
- Animals, Disease Models, Animal, Electromyography, Female, Fluorescent Antibody Technique, Mice, Motor Neurons metabolism, Muscle, Skeletal innervation, Musculocutaneous Nerve transplantation, Neuromuscular Junction physiology, Sensory Receptor Cells metabolism, Treatment Outcome, Ulnar Nerve transplantation, Brachial Plexus Neuropathies etiology, Brachial Plexus Neuropathies surgery, Nerve Transfer methods
- Abstract
Nerve transfer involves the use of a portion of a healthy nerve to repair an injured nerve, and the process has been used to alleviate traumatic brachial plexus injuries in humans. Study of the neural mechanisms that occur during nerve transfer, however, requires the establishment of reliable experimental models. In this study, we developed an ulnar-musculocutaneous nerve-transfer model wherein the biceps muscle of a mouse was re-innervated using a donor ulnar nerve. Similar muscle action potentials were detected in both the end-to-end suture of the transected nerve (correctrepair) group and the ulnar-musculocutaneous nerve-transfer group. Also, re-innervated acetylcholine receptor (AChR) clusters and muscle spindles were observed in both procedures. There were fewer re-innervated AChR clusters in the nerve transfer group than in the correct repair group at 4 weeks, but the numbers were equal at 24 weeks following surgery. Thus, our ulnar-musculocutaneous nerve-transfer model allowed physiological and morphological evaluation for re-innervation process in mice and revealed the delay of this process during nerve transfer procedure. This model will provide great opportunities to study regeneration, re-innervation, and functional recovery induced via nerve transfer procedures.
- Published
- 2019
- Full Text
- View/download PDF
34. Influence of Ultra-High Hydrostatic Pressurizing Brown Rice on Cognitive Functions and Mental Health of Elderly Japanese Individuals: A 2-Year Randomized and Controlled Trial.
- Author
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Kuroda Y, Matsuzaki K, Wakatsuki H, Shido O, Harauma A, Moriguchi T, Sugimoto H, Yamaguchi S, Yoshino K, and Hashimoto M
- Subjects
- Aged, Cognition Disorders etiology, Diet adverse effects, Female, Humans, Hydrostatic Pressure, Japan, Male, Protective Factors, Cognition, Cooking methods, Diet methods, Mental Health, Oryza
- Abstract
Brown rice contains many ingredients that might protect against cognitive decline and Alzheimer's disease. However, brown rice is very hard, difficult to cook, and is poorly digested; thus, it is difficult to eat long-term. To solve these problems, ultra-high hydrostatic pressurizing brown rice (UHHPBR) was prepared. We investigated the effects of dietary UHHPBR administration for 24 mo on cognitive function and mental health in the elderly. Healthy elderly participants (n=52) were randomized into UHHPBR and polished white rice (WR) groups. The UHHPBR group consumed 100 g of UHHPBR per day for 24 mo and the WR group consumed white rice. At baseline, and after 12 and 24 mo, cognitive functions were assessed using the Revised Hasegawa's Dementia Scale, Mini-Mental State Examination, Frontal Assessment Battery (FAB), and the Cognitive Assessment for Dementia, iPad version (CADi). Mental health condition was also assessed using the Apathy Scale and the Zung Self-Rating Depression Scale, and serum biochemical parameters were determined. From baseline to month 24, the mean change in the FAB-sub item 1 scores was higher in the UHHPBR group compared to the WR group. Furthermore, apathy scores decreased, as well as the time required to answer all questions of the CADi, and plasma epinephrine levels increased. These results indicate that a 2-y oral consumption of UHHPBR increases information processing speed (as a measure of cognitive function) and improves apathy in the elderly, suggesting a protective effect of UHHPBR administration against age-related decline in brain cognition and motivation.
- Published
- 2019
- Full Text
- View/download PDF
35. Causes of variation among rice models in yield response to CO 2 examined with Free-Air CO 2 Enrichment and growth chamber experiments.
- Author
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Hasegawa T, Li T, Yin X, Zhu Y, Boote K, Baker J, Bregaglio S, Buis S, Confalonieri R, Fugice J, Fumoto T, Gaydon D, Kumar SN, Lafarge T, Marcaida Iii M, Masutomi Y, Nakagawa H, Oriol P, Ruget F, Singh U, Tang L, Tao F, Wakatsuki H, Wallach D, Wang Y, Wilson LT, Yang L, Yang Y, Yoshida H, Zhang Z, and Zhu J
- Subjects
- Climate Change, Crops, Agricultural drug effects, Crops, Agricultural growth & development, Models, Biological, Nitrogen pharmacology, Oryza drug effects, Plant Leaves anatomy & histology, Carbon Dioxide pharmacology, Oryza growth & development
- Abstract
The CO
2 fertilization effect is a major source of uncertainty in crop models for future yield forecasts, but coordinated efforts to determine the mechanisms of this uncertainty have been lacking. Here, we studied causes of uncertainty among 16 crop models in predicting rice yield in response to elevated [CO2 ] (E-[CO2 ]) by comparison to free-air CO2 enrichment (FACE) and chamber experiments. The model ensemble reproduced the experimental results well. However, yield prediction in response to E-[CO2 ] varied significantly among the rice models. The variation was not random: models that overestimated at one experiment simulated greater yield enhancements at the others. The variation was not associated with model structure or magnitude of photosynthetic response to E-[CO2 ] but was significantly associated with the predictions of leaf area. This suggests that modelled secondary effects of E-[CO2 ] on morphological development, primarily leaf area, are the sources of model uncertainty. Rice morphological development is conservative to carbon acquisition. Uncertainty will be reduced by incorporating this conservative nature of the morphological response to E-[CO2 ] into the models. Nitrogen levels, particularly under limited situations, make the prediction more uncertain. Improving models to account for [CO2 ] × N interactions is necessary to better evaluate management practices under climate change.- Published
- 2017
- Full Text
- View/download PDF
36. Pleiotropic properties of ASK1.
- Author
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Sakauchi C, Wakatsuki H, Ichijo H, and Hattori K
- Subjects
- Animals, Disease, Humans, Models, Biological, Protein Kinase Inhibitors pharmacology, Genetic Pleiotropy, MAP Kinase Kinase Kinase 5 metabolism
- Abstract
Background: Apoptosis signal-regulating kinase 1 (ASK1), also known as mitogen-activated protein kinase kinase kinase 5 (MAP3K5), has the potential to induce cellular apoptosis under various physiological conditions. It has long been suggested that ASK1 is highly sensitive to oxidative stress and contributes substantially to apoptosis. However, recent studies have indicated that ASK1 has pleiotropic roles in living organisms through other mechanisms in addition to apoptosis., Scope of the Review: This review describes the physiological functions of ASK1 in living organisms, focusing on the regulatory mechanisms of ASK1 activity and its importance in the pathogenesis of various diseases. We also highlight recent works published within the past few years., Major Conclusions: ASK1 forms a high-molecular-mass complex within the cell, designated as the ASK1 signalosome. Soon after the discovery of ASK1, several regulatory components of the ASK1 signalosome have been revealed, including thioredoxin (Trx), tumor-necrosis factor α receptor-associated factors (TRAFs) and 14-3-3s. In parallel with the precise analyses unveiling the molecular basis of ASK1 regulation, the physiological or pathophysiological significance of ASK1 in diverse organs has been elucidated. In addition to the generation of global knockout mice or tissue-specific knockout mice, ASK1-specific inhibitors have illuminated the biological roles of ASK1., General Significance: The multi-faceted features of the function of ASK1 have been discovered over the past two decades, revealing that ASK1 is a crucial molecule for maintaining cellular homeostasis, especially under conditions of stress. Based on the results that ASK1 deficiency provides beneficial effects for several diseases, modulating ASK1 activity is a promising method to ameliorate a subset of diseases., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
37. Rice cultivar responses to elevated CO 2 at two free-air CO 2 enrichment (FACE) sites in Japan.
- Author
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Hasegawa T, Sakai H, Tokida T, Nakamura H, Zhu C, Usui Y, Yoshimoto M, Fukuoka M, Wakatsuki H, Katayanagi N, Matsunami T, Kaneta Y, Sato T, Takakai F, Sameshima R, Okada M, Mae T, and Makino A
- Abstract
There is some evidence that rice cultivars respond differently to elevated CO2 concentrations ([CO2]), but [CO2]×cultivar interaction has never been tested under open-field conditions across different sites. Here, we report on trials conducted at free-air CO2 enrichment (FACE) facilities at two sites in Japan, Shizukuishi (2007 and 2008) and Tsukuba (2010). The average growing-season air temperature was more than 5°C warmer at Tsukuba than at Shizukuishi. For four cultivars tested at both sites, the [CO2]×cultivar interaction was significant for brown rice yield, but there was no significant interaction with site-year. Higher-yielding cultivars with a large sink size showed a greater [CO2] response. The Tsukuba FACE experiment, which included eight cultivars, revealed a wider range of yield enhancement (3-36%) than the multi-site experiment. All of the tested yield components contributed to this enhancement, but there was a highly significant [CO2]×cultivar interaction for percentage of ripened spikelets. These results suggest that a large sink is a prerequisite for higher productivity under elevated [CO2], but that improving carbon allocation by increasing grain setting may also be a practical way of increasing the yield response to elevated [CO2].
- Published
- 2013
- Full Text
- View/download PDF
38. Laser trapping of deformable objects.
- Author
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Murazawa N, Juodkazis S, Misawa H, and Wakatsuki H
- Abstract
We report the trapping and manipulation of bubbles in viscous glass melts through the use of a laser. This phenomenon is observed in bubbles tens of micrometers in diameter under illumination by low numerical aperture (NA = 0.55). Once the bubble was centered on the optical axis, it was trapped and followed a lateral relocation of the laser beam. This phenomenon is explained by modifications of the bubble's shape induced by axial heating and a decrease in surface tension. It is shown that formation of a concave dimple on the bubble's front surface explains the observed laser trapping and manipulation. This mechanism of laser trapping is expected to take place in other deformable materials and can also be used to remove bubbles from melts or liquids. For this technique to be effective, the alteration of the bubble's shape should be faster than its expulsion out of the laser's point of focus.
- Published
- 2007
- Full Text
- View/download PDF
39. Tributyltin disturbs bovine adrenal steroidogenesis by two modes of action.
- Author
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Yamazaki T, Shimodaira M, Kuwahara H, Wakatsuki H, Horiuchi H, Matsuda H, and Kominami S
- Subjects
- Animals, Cattle, Cells, Cultured, Cholesterol Side-Chain Cleavage Enzyme antagonists & inhibitors, Cholesterol Side-Chain Cleavage Enzyme metabolism, Gene Expression Regulation drug effects, Microsomes enzymology, Mitochondria metabolism, Phosphoproteins genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Zona Fasciculata enzymology, Adrenal Cortex Hormones biosynthesis, Trialkyltin Compounds pharmacology, Zona Fasciculata drug effects
- Abstract
Tributyltin, an environmental pollutant, affected adrenal steroid hormone biosynthesis by two modes of action. Treatment of bovine adrenal cultured cells with 10-100 nM tributyltin for 48 h suppressed cortisol and androstenedione secretion, but induced the accumulation of 17alpha-hydroxyprogesterone and deoxycortisol, indicating that the P450(C21) and P450(11beta) activities were specifically suppressed. Direct inhibition of the enzymatic activities due to tributyltin was not observed in isolated organelles of untreated cells at concentrations less than 10 microM. Western blotting experiments using specific antibodies against steroidogenic enzymes showed that treatment with 1-100 nM tributyltin caused a decrease in cellular P450(C21) and P450(11beta) protein levels, and real-time PCR experiments showed that the decrease in protein content was attributable to decreases in mRNA of the enzymes. Tributyltin at concentrations higher than 100 nM suppressed all steroid biosynthesis in the adrenal cells. This suppression was closely correlated to the decrease in steroidogenic acute regulatory protein. Since nanomolar concentrations of tributyltin disturbed steroidogenesis in mammalian cells, there is the possibility that steroid hormone synthesis in polluted wild animals is affected by this compound.
- Published
- 2005
- Full Text
- View/download PDF
40. [The consequence after introduction of clean intermittent catheterization (CIC) in children with neurogenic bladder dysfunction secondary to spina bifida--the comparison of patients with and without upper urinary tract dilation at the time CIC was introduced].
- Author
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Obara K, Komeyama T, Mizusawa T, Tsutsui T, Wakatsuki H, Arai K, Kosegawa E, Suzuki K, and Takahashi K
- Subjects
- Adolescent, Child, Child, Preschool, Dilatation, Pathologic therapy, Female, Follow-Up Studies, Humans, Infant, Infant, Newborn, Male, Prognosis, Urinary Bladder, Neurogenic etiology, Urodynamics, Spinal Dysraphism complications, Urinary Bladder, Neurogenic therapy, Urinary Catheterization methods, Urinary Tract pathology
- Abstract
Purpose: The aim of current study was to review the consequence after introduction of clean intermittent catheterization (CIC) in children with neurogenic bladder dysfunction secondary to spina bifida., Patients and Methods: We retrospectively reviewed the records of 34 children (19 girls and 15 boys) presenting our clinic in a 18-year period. The patients were divided concentrating on the radiological upper urinary tract findings when CIC was introduced. 18 children had dilated upper urinary tract. In these patients, 10 children already had dilated upper urinary tract at first visiting to our clinic(group A). In remaining 8 patients, dilatation of upper urinary tract was found out in the course of followup (group B). 16 children had normal upper urinary tract when CIC was introduced. In 7 patients, CIC was applied for post-void residual and urinary tract infection (group C). In remaining 9 patients, CIC was introduced for urodynamically low compliance bladder (group D)., Results: In group A, 5 patients underwent enterocystoplasty and 3 patients underwent anti-reflux surgery consequently. Two patients, including 1 patient who underwent enterocystoplasty, have chronic renal dysfunction. In group B, 3 patients underwent enterocystoplasty and 2 patients underwent anti-reflux surgery. In group C, all patients have normal upper urinary tract. In group D, 8 patients have normal upper urinary tract. However, 1 patients underwent enterocystoplasty for low compliance bladder with vesicoureteral reflux (VUR)., Conclusion: Some patients show the improvement of dilated upper urinary tract or VUR after introduction of CIC. However, enterocystoplasty or anti-reflux surgery was needed for many patients to prevent upper urinary tract deterioration. The patients whom CIC was introduced for postvoid residual and urinary tract infection have not shown any deterioration of upper urinary tract. The efficacy of CIC for incontinence was poor because many patients have urethral sphincter incompetence.
- Published
- 2003
- Full Text
- View/download PDF
41. [A case of rectal bladder construction by Duhamel procedure for vesical extrophy].
- Author
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Osawa T, Kawakami Y, Wakatsuki H, and Hoshii T
- Subjects
- Child, Preschool, Humans, Male, Bladder Exstrophy surgery, Rectum surgery, Urinary Bladder surgery, Urologic Surgical Procedures methods
- Abstract
A 4 year old boy, who underwent 2 times of unsuccessful primary closure of the extrophied bladder at 7 days and 5 months after birth, was treated with construction of a rectal bladder with sigmoid-pull through by Duhamel procedure. Vas deference, seminal vesicles and prostate were not found at the cystectomy operation. Epispadias urethral mucosa was removed and phalloplasty was performed using the ventral hooded foreskin. The results were cosmetically and functionally excellent. He could keep continence and his upper urinary tract was normal at 6 months after the operation.
- Published
- 2001
- Full Text
- View/download PDF
42. Suppression of the induction of long-term depression by carbon monoxide in rat cerebellar slices.
- Author
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Shibuki K, Kimura S, and Wakatsuki H
- Subjects
- Aminolevulinic Acid pharmacology, Animals, Cerebellum cytology, Cerebellum drug effects, Cyclic GMP metabolism, Excitatory Postsynaptic Potentials drug effects, Excitatory Postsynaptic Potentials physiology, Female, Long-Term Potentiation physiology, Male, Neural Inhibition physiology, Nitric Oxide metabolism, Organ Culture Techniques, Oxygen Consumption drug effects, Oxygen Consumption physiology, Photosensitizing Agents pharmacology, Porphobilinogen pharmacology, Rats, Rats, Wistar, Signal Transduction drug effects, Signal Transduction physiology, Synaptic Transmission drug effects, Synaptic Transmission physiology, Carbon Monoxide pharmacology, Long-Term Potentiation drug effects, Neural Inhibition drug effects, Purkinje Cells drug effects, Purkinje Cells metabolism
- Abstract
Carbon monoxide (CO) suppresses brain functions at doses lower than that suppressing oxygen (O(2)) supply to the brain, and the cerebellum is one of the sites most susceptible to the neurotoxic effects of CO. We investigated the effects of CO on the induction of cerebellar long-term depression (LTD) in the synapses between parallel fibres (PFs) and Purkinje cells. CO, at concentrations between 8 nM and 5 microM, exhibited almost no effect on synaptic responses in Purkinje cells, O(2) consumption and NO release from PFs in rat cerebellar slices. However, the induction of LTD was significantly suppressed by CO at concentrations between 40 and 200 nM. The suppressive effect of 40 nM CO was antagonized by 10 microM NOR3, an NO donor. In contrast, CO exhibited no clear effect on the induction of LTD at concentrations between 1 and 5 microM. The induction of LTD, suppressed by 10 microM N(G)-nitro-L-arginine, an inhibitor of NO synthase, was not restored by 5 microM CO. CO is not only a neurotoxic substance but also a candidate for an intercellular messenger. delta-Aminolevulinate (30 microM), a substance facilitating endogenous CO production, suppressed the induction of LTD, and the effect of delta-aminolevulinate was antagonized by 10 microM NOR3. These findings suggest that CO may have a suppressive effect on the induction of cerebellar LTD at nanomolar concentrations, probably via its effects on NO/cGMP signalling.
- Published
- 2001
- Full Text
- View/download PDF
43. Layer-specific NO dependence of long-term potentiation and biased NO release in layer V in the rat auditory cortex.
- Author
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Wakatsuki H, Gomi H, Kudoh M, Kimura S, Takahashi K, Takeda M, and Shibuki K
- Subjects
- Animals, Auditory Cortex cytology, Auditory Cortex drug effects, Cyclic GMP analogs & derivatives, Cyclic GMP pharmacology, Electric Stimulation, Excitatory Amino Acid Agonists pharmacology, Female, Immunohistochemistry, In Vitro Techniques, Male, Membrane Potentials physiology, N-Methylaspartate pharmacology, Nerve Tissue Proteins metabolism, Neurons drug effects, Neurons physiology, Nitric Oxide metabolism, Nitric Oxide Synthase metabolism, Nitric Oxide Synthase Type I, Rats, Rats, Wistar, Signal Transduction physiology, Auditory Cortex physiology, Long-Term Potentiation physiology, Nitric Oxide physiology
- Abstract
1. We investigated the role of nitric oxide (NO) in the induction of long-term potentiation (LTP) in slices prepared from the rat auditory cortex. 2. Tetanic stimulation of layer IV elicited LTP of field potentials in layer II-III (LTPII-III) and in layer V (LTPV). The magnitude of LTPII-III measured at 30 min after tetanic stimulation was 171 +/- 9% (n = 15, mean +/- s.e.m.) of the control measured before tetanic stimulation, while that of LTPV was 138 +/- 3% (n = 17). 3. NO synthase (NOS) inhibitors had no apparent effect on LTPII-III, but LTPV was significantly suppressed (P < 0.001). This suppression of LTPV was significantly antagonized by a NO donor (P < 0.001) or a cGMP analogue (P < 0.001). 4. Small non-pyramidal neurones in the auditory cortex were stained with an anti-neuronal NOS antibody. More neurones were stained with the antibody in the deeper cortical layers. 5. We measured neocortical NO release with electrochemical NO probes. Layer IV stimulation elicited significantly more NO release in layer V than in layer II-III (P < 0.001). The amplitude of the increase in NO concentration elicited by stimulation at 20 Hz for 5 s was 380 +/- 14 pM (n = 55) in layer V and 55 +/- 8 pM (n = 5) in layer II-III. 6. NO release in layer V was partially but significantly suppressed by non-NMDA (P < 0.002) or NMDA (P < 0.002) receptor antagonists. Simultaneous application of the antagonists of the two types blocked NO release almost completely. 7. These results clearly indicate the NO dependence of the induction of LTPV, and the greater NO release in the deeper layer of the rat auditory cortex.
- Published
- 1998
- Full Text
- View/download PDF
44. Deficient cerebellar long-term depression, impaired eyeblink conditioning, and normal motor coordination in GFAP mutant mice.
- Author
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Shibuki K, Gomi H, Chen L, Bao S, Kim JJ, Wakatsuki H, Fujisaki T, Fujimoto K, Katoh A, Ikeda T, Chen C, Thompson RF, and Itohara S
- Subjects
- Animals, Immunoblotting, Mice, Mice, Mutant Strains, Microscopy, Electron, Motor Activity physiology, Synapses ultrastructure, Time Factors, Cerebellum physiopathology, Eye physiopathology, Glial Fibrillary Acidic Protein analysis
- Abstract
Mice devoid of glial fibrillary acidic protein (GFAP), an intermediate filament protein specifically expressed in astrocytes, develop normally and do not show any detectable abnormalities in the anatomy of the brain. In the cerebellum, excitatory synaptic transmission from parallel fibers (PFs) or climbing fibers (CFs) to Purkinje cells is unaltered, and these synapses display normal short-term synaptic plasticity to paired stimuli in GFAP mutant mice. In contrast, long-term depression (LTD) at PF-Purkinje cell synapses is clearly deficient. Furthermore, GFAP mutant mice exhibited a significant impairment of eyeblink conditioning without any detectable deficits in motor coordination tasks. These results suggest that GFAP is required for communications between Bergmann glia and Purkinje cells during LTD induction and maintenance. The data support the notion that cerebellar LTD is a cellular mechanism closely associated with eyeblink conditioning, but is not essential for motor coordination tasks tested.
- Published
- 1996
- Full Text
- View/download PDF
45. Prolonged suppression of gonadotropin secretion after weight recovery in an anorectic patient with Turner's syndrome: reduced gonadal function in anorexia nervosa is independent in part on nutrition.
- Author
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Ohzeki T, Egi S, Kagawa J, Nagafuchi S, Igarashi Y, Hanaki K, Ishitani N, Motozumi-Wakatsuki H, and Sunaguchi M
- Subjects
- Adolescent, Anorexia Nervosa complications, Anorexia Nervosa physiopathology, Female, Follicle Stimulating Hormone blood, Humans, Hypogonadism metabolism, Hypogonadism physiopathology, Luteinizing Hormone blood, Turner Syndrome complications, Anorexia Nervosa metabolism, Gonadotropins, Pituitary metabolism, Hypogonadism etiology, Nutritional Physiological Phenomena physiology, Turner Syndrome metabolism, Weight Gain physiology
- Abstract
Two hypotheses have been postulated as to the pathogenesis of hypogonadotropinemia in anorexia nervosa; one is starvation and weight loss and the other is a psychological factor to influence gonadotropin secretion. Our patient suffered from very rare concurrence of Turner's syndrome and anorexia nervosa and a study of this experiment in nature provided important evidences concerning decreased secretion of gonadotropins in the eating disorder. The patient was diagnosed as Turner's syndrome when she was 6 years old. Her gonadotropin levels were elevated to the castrated ranges (LH 61.8 IU/l; FSH 175.8 IU/l) after 8 years of age. She was noticed to be anorectic at the age of 13 years. Serum levels of the pituitary gonadotropins were lowered (LH 2.9 IU/l; FSH 3.0 IU/l) and their responses to luteinizing hormone-releasing hormone were decreased beneath the normal prepubertal limits. After one year of the anorectic period, she recovered the weight though her gonadotropin levels remained in the very low ranges (LH 2.7 IU/l; FSH 2.5 IU/l). The results suggest that hypogonadism in anorexia nervosa is not solely caused by nutritional deficiency but rather by other factors such as psychological abnormalities.
- Published
- 1989
- Full Text
- View/download PDF
46. Ultraviolet spectrophotometric determination of benzoic acid in soy sauce.
- Author
-
Goo RK, Wakatsuki H, and Kanai H
- Subjects
- Condiments analysis, Spectrophotometry, Ultraviolet, Benzoates analysis, Food Additives analysis
- Abstract
Proteins and other interfering substances are precipitated from soy sauce, using sodium tungstate under acidic conditions. After centrifugation, the supernate is successively extracted with ethyl ether to isolate the benzoic acid in the organic solvent. The ethyl ether extract is washed with dilute HCl. Benzoic acid is then quantitatively determined by ultraviolet spectrophotometry. Recovery of sodium benzoate added to soy sauce ranged from 94 to 104%.
- Published
- 1979
47. Spectrophotometric determination of urea in urine stains on foods and containers.
- Author
-
Goo RK, Kanai H, Inouye V, and Wakatsuki H
- Subjects
- Animals, Rodentia, Spectrophotometry, Urea urine, Food Contamination analysis, Urea analysis
- Abstract
Urea is extracted from rodent urine-contaminated material with hot acetone. After the extract is evaporated to dryness, aqueous urease solution is added to produce ammonia and carbon dioxide. A blue product, indophenol, is formed by the reaction of ammonia with phenol in the presence of hypochlorite. Absorbance is maximum at 625 nm. Presence of urea is easily detected at 4 microgram. Detection of urine contamination in various materials is compared with detection by the AOAC urease-H2PtCl6 test.
- Published
- 1980
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