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Objective To investigate the efficacy and safety of disintegrin DTY29 on inhibiting the proliferation of rabbit lens epithelial cells (LECs) in vitro and in vivo. Methods (l) In vitro: 36-well plates were divided into control group (a) and experimental groups (b, c and d), nine holes each group. The human LECs were cultured and scratched, and then culture media, 0.08 µmol/L, 0.4 µmol/L, and 2 µmol/L DTY29 were added into 4 groups, respectively. Scratch widths were photographed and measured at 0 h, 6h, 12h and 24 h. (2) In vivo: 36 male New Zealand white rabbits were randomly divided into control group (A), experimental groups (B, C and D), nine rabbits each group. Amounts of corneal endothelial cells were calculated before operative. Phacoemulsification was performed in all right eyes of the rabbits, and then 0.2 mL of Ringer's, 10 µ mol/L, 20 µmol/L and 40 µmol/L DTY29 solution was injected into the anterior chamber of groups A, B, C and D, respectively. Cornea, anterior chamber, pupil, posterior capsular opacification (PCO), and fundus were observed postoperatively. Results At6h, 12 h and 24 h of cells scratch test in vitro, the scratch widths of group a reduced significantly (P < 0.05) compared with groups b, c and d. There were no significant differences in corneal endothelial cells loss among groups A, B and C (P > 0.05), while a significant reduction was found in group D compared with groups A,B and C (< 0.05). PCO was observed in both groups A and B after 1-3 months postoperatively, but there was no significant difference in incidence (> 0.05). PCO hadn't occurred in groups C and D. Retinal detachment,choroidal detachment and other serious complications were not observed. Conclusion Disintegrin DTY29 could inhibit lens epithelial cells proliferation in vitro or in vivo,and there was a positive correlation between inhibiting effect and concentration. In vivo, corneal endothelial cells can be destroyed by DTY29 with a high concentration, and no side effects on intraocular tissues have been observed, which demonstrated the safety at a low concentration. The experiment results suggested that the optimum concentration ranged from 10 µmol/L to 20 µmol/L,and further investigation should be done to prove its safety and optimum concentration. [ABSTRACT FROM AUTHOR]

Objective To investigate the clinical effects of angulated distal suture trabeculectomy on primary angle-closure glaucoma (PACG) . Methods We selected 105 eyes of 85 inpatients suffering from PACG in our department from Sept. 2011 to Dec. 2013 as research object. The enrolled patients were randomly divided into observation group and control group. Patients in observation group were treated with angulated distal suture trabeculectomy while patients in control group were treated with Traditional trabeculectomy. Then we observed and followed up shallow anterior chamber, functional filtering bleb, success rate of operation and the incidence of complication in the two groups. Results In the formation of functional filtering bleb and success rate of operation between the two groups, there was no statistically significant difference 3 months after operation (P> 0.05), while there were statistically significant differences 6 months and 1 year after operation (P < 0.05). In early-stage complications such as ocular hypotony, shallow anterior chamber in the corresponding period between the two groups, there was no statistically significant difference (P> 0.05) . There were no suprachorodal hemorrhage and malignant glaucoma in the two groups. Conclusion Angulated distal suture trabeculectomy is more helpful for long-dated functional filtering bleb formation and better in controlling intraocular pressure in PACG, it is a more ideal operation method. [ABSTRACT FROM AUTHOR]