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Abstract Background Pueraria is a widely cultivated medicinal and edible homologous plant in Asia, and its tuberous roots are commonly used in the food, nutraceutical, and pharmaceutical industries. “Gange No. 5” is a local variety of Pueraria montana var. thomsonii (Bentham) M.R. Almeida (PMT) in Jiangxi Province, China. After optimizing its cultivation technique, we shortened the cultivation cycle of this variety from two years to one year, suggesting that the regulatory mechanism of the endogenous hormone system during tuberous root expansion may have changed significantly. In this study, we focused on the molecular mechanisms of endogenous hormones in promoting tuberous root expansion during one-year cultivation of “Gange No. 5”. Results The mid-late expansion period (S4) is critical for the rapid swelling of “Gange No. 5” tuberous roots during annual cultivation. At S4, the number of cells increased dramatically and their volume enlarged rapidly in the tuberous roots, the fresh weight of a single root quickly increased, and the contents of multiple nutrients (total protein, total phenol, isoflavones) and medicinal components (puerarin, puerarin apigenin, and soy sapogenin) were at their peak values. We compared the transcriptomes and metabolomes of S1 (the pre-expansion period), S4, and S6 (the final expansion period), and screened 42 differentially accumulated hormone metabolites and 1,402 differentially expressed genes (DEGs) associated with hormone biosynthesis, metabolism, and signaling. Most Auxin, cytokinins (CKs), jasmonic acids (JAs), salicylic acid (SA), melatonin (MLT), and ethylene (ETH), reached their maximum levels at S1 and then gradually decreased; however, abscisic acid (ABA) appeared in S6, indicating that most of the endogenous hormones may play a key role in regulating the initiation of tuberous root expansion, while ABA mainly promotes tuberous root maturation. Notably, multiple key genes of the ‘Tryptophan metabolism’ pathway (ko00380) were significantly differentially expressed, and COBRA1, COBRA2, YUCCA10, IAA13, IAA16, IAA20, IAA27, VAN3, ACAA2, and ARF were also identified to be significantly correlated with the expansion of “Gange No. 5” tuberous roots. Conclusions Our study has revealed how endogenous hormone regulation affects the expansion of “Gange No. 5” tuberous roots. These findings offer a theoretical foundation for improving the yield of PMT tuberous roots.

Obstructive sleep apnea and chronic airway inflammatory diseases, such as chronic obstructive pulmonary disease (COPD) and asthma, are prevalent respiratory conditions, particularly among the elderly. These diseases significantly impact the health and quality of life of the general population. The coexistence of two or more of these conditions, known as overlap syndrome, exacerbates their impact and is frequently encountered in clinical practice. This article provides a comprehensive review of the progress in understanding overlap syndromes, focusing on their distinctive disease features, risk factors, pathophysiological mechanisms, symptoms, diagnostic approaches, and corresponding treatment strategies.

ObjectivesThe advanced exergoeconomics analysis method based on exergetic analysis development can refine the economic costs of splitting system components and deeply explore the underlying reasons for the formation of economic costs.MethodsCombining advanced exergetic analysis, advanced exergoeconomics analysis method is used to split the costs of the components in the gas-steam combined cycle power generation system into endogenous, exogenous, avoidable and unavoidable costs, and calculate them.ResultsUnder the design conditions, the avoidable loss in the combustion chamber in the combined cycle power system is the largest, which is 28.41 MW, accounting for 26.55% of the combustion chamber loss. Based on the results of the analysis, different improvement measures are proposed for the turbine to reduce the endogenous and exogenous losses of the system. The largest share of the annualized cost of the system is the endogenous avoidable portion, and the bottom-cycle improvement is prioritized highest for the high-pressure cylinder, followed by the low-pressure cylinder. The exogenous share of annualized costs in the combined cycle power system is 80.59%, of which the exogenous avoidable portion is 40.04%.ConclusionsThe findings of the study can provide the system with a multifaceted energy efficiency evaluation perspective and an improvement direction to optimize the cost.

Objective The international standards adopted in metro vehicle design and verification in China do not fully align with the actual vehicle operating conditions, resulting in the phenomenon of fatigue cracks in some metro vehicle bogie frames before reaching the specified service mileage. It is therefore necessary to study the load characteristics of metro vehicle bogie frames under actual operating conditions. Method Based on long-term tracking test data of B-type metro vehicles, a combination of statistical calculation and STFT (short-time Fourier transform) analysis is used to study the variation law of bogie frame loads from three aspects of line condition, vehicle operation state and passenger load. Result & Conclusion The main load of the bogie frame is highly correlated with line conditions, while local load of the frame are closely related to the vehicle operation state. There is a significant negative linear correlation between damper load and passenger load, while other bogie frame loads, except for lateral rolling loads, show a positive linear correlation with passenger load.

ObjectiveTo explore the risk of different levels of pre-pregnancy obesity on trimester-specific thyroid dysfunction.MethodsQuestionnaire information， blood samples， and urine samples from a 2017 pregnancy cohort study in Shanghai， China were collected. A total of 2 455 pregnant women were included in the analysis. Pre-pregnancy BMI was calculated based on the height and self-reported pre-pregnancy weight. Serum TSH， total thyroxine （TT4）， free thyroxine （FT4）， total triiodothyronine （TT3）， free triiodothyronine （FT3）， thyroid globulin antibody（TgAb）， and Thyroid peroxidase antibody （TPOAb） were measured using the electrochemiluminescence method. Urine iodine levels were measured using the acid digestion method. Levels of thyroid function indexes of pregnant women with different degrees of obesity during pre-pregnancy were compared， and trimester-specific thyroid dysfunction was evaluated according to the reference range of trimester-specific thyroid hormone established by this cohort. Multivariate logistic regressions analysis was used to assess the correlation between pre-pregnancy obesity and trimester-specific thyroid dysfunction.ResultsAs the degree of obesity increased， maternal levels of FT3 and TT3 gradually increased during pregnancy （P

Two-dimensional layered semiconductors have attracted intense interest in recent years. The van der Waals coupling between the layers tolerates stacking various materials and establishing heterostructures with new characteristics for a wide range of optoelectronic applications. The interlayer exciton dynamics at the interface within the heterostructure are vitally important for the performance of the photodetector and photovoltaic device. Here, a heterostructure comprising two-dimensional organic-inorganic Ruddlesden–Popper perovskites and transition metal dichalcogenide monolayer was fabricated and its ultrafast charge separation processes were systematically studied by using femtosecond time-resolved transient absorption spectroscopy. Significant hole and electron transfer processes in the ps and fs magnitude at the interface of the heterostructure were observed by tuning pump wavelengths of the pump-probe geometries. The results emphasize the realization of the exciton devices based on semiconductor heterostructures of two-dimensional perovskite and transition metal dichalcogenide.

This article presents a review on the effect of family environmental factors on adolescent depression patients， in which the domestic and foreign literature on the family environment of adolescent depression patients are thoroughly reviewed with the emphasis of the relevant concepts， assessment tools， impact effects and potential mechanisms， and the research progress， existing limitations and directions for future research are summarized. Additionally， the awareness of the negative family environment associated with depression derives specific reference values for the early identification and prevention of adolescent depression.

Objective To investigated the expression of metabolism-related genes (MRGs) in kidney renal clear cell carcinoma (KIRC) and its correlation with prognosis of patients so as to search for potential intervention targets. Methods Bioinformatics were used to mine KIRC transcriptome data in the Cancer Genome Atlas Program (TCGA) database, and the MRGs abnormally expressed were obtained, among which the ones closely associated with overall survival of KIRC patients were screened by LASSO and Cox regression. Furthermore, Cox regression analysis was adopted to construct a risk-score model to predict the prognosis of KIRC patients, and its predictive ability was evaluated. Finally, the expression characteristics of prognostic related MRGs were verified using RT-qPCR in KIRC cell line A498 and renal tubular epithelial cell line HK-2, respectively. Results A total of 875 differentially expressed MRGs were found between KIRC and adjacent tissues. Univariate Cox regression analysis showed that 38 MRGs significantly affected the prognosis of patients, and 7 of them (CNGB1, CACNA1I, KCNT1, KCNH1, PLA2G4D, CHST6, and ATP2A1) were further selected as prognostic risk factors by LASSO and multivariate Cox regression. Subsequently, a risk-score model was constructed based on the above 7 factors. The score obtained from the model could be used as an independent risk factor to predict the prognosis of KIRC patients (risk ratio: 4.671, P < 0.001), while age, gender, and tumor grade were not correlated with the prognosis of patients (P>0.05). Conclusion There are several MRGs with abnormal expression in KIRC, and the prognostic risk model based on 7 genes screened from the MRGs can effectively predict the prognosis of KIRC patients, providing a new basis for future diagnosis and treatment of KIRC.

Renewable energy is widely used in combined cooling, heating and power (CCHP) systems. This is important for building a low-carbon, flexible, multi-energy complementary energy system. However, coupling different renewable energy sources can have a somewhat differentiated impact on the performance of the system. In this study, an approach combining a long short-term memory (LSTM) network with multiple optimization algorithms is proposed. Comparative performance analysis of CCHP systems coupling solar and wind subsystems is conducted. Firstly, the renewable energy output is predicted by LSTM. Then, the Pareto frontiers of the coupled renewable energy CCHP system are generated by the Non-dominated Genetic Sorting Algorithm. The results are fed into the distance between superior and inferior solution methods to arrive at a decision, completing the multi-objective optimization of the system. Results show that the CCHP system coupling photovoltaic (PV) and solar collector (ST) is superior to the CCHP system coupling photovoltaic-photovoltaic-thermal integrated device. The system performance can be further improved by adding wind turbines to the integrated system coupling PV and ST.

Epithelial ovarian cancer (EOC) is the leading cause of deaths due to cancer in women. Adipocytes have been suggested to play a key role in the stimulation of EOC growth. However, the mechanisms underlying the adipocyte-induced EOC proliferation remain undefined. Here, we provide the first evidence that adipocytes induce the activation of sphingosine kinase (SphK) 2 in EOC, which represents a novel pathway that mediates the adipocyte-induced EOC growth. SphK2 inhibition in EOC cells led to a remarkable inhibition of the adipocyte-induced cell proliferation. Moreover, the adipocyte-induced SphK2 activation in EOC cells was extracellular signal-regulated protein kinases (ERK) dependent. Furthermore, silencing SphK2 in EOC significantly inhibited the adipocyte-induced expression of phospho-ERK and c-Myc, two crucial players in EOC growth. Collectively, the current study unraveled a previously unrecognized role of SphK2 in the adipocyte-induced growth-promoting action in EOC, suggesting a novel target for EOC treatment.

In order to explore the specific path of the influence of job autonomy on the satisfaction of knowledge employees, the current study deduced and established a Chain Mediation Model, which was based on the Resource-Gain-Development Model and the Conservation of Resources Theory. Primary data were gathered through questionnaire surveys at several cities in China by using a professional platform named “Wenjuanxing.” The target populations were employees with a bachelor’s degree or above, who were engaged in higher knowledge content and have mastered certain professional knowledge and skills, including technical R & D personnel, management personnel, professionals (such as accountants, lawyers, and medics) and other personnel generally recognized by the academic community. In order to improve the reliability of the sample and reduce the error caused by regional differences, the questionnaires were disseminated to involve as many cities in China as possible, such as Tianjin, Beijing, Chengdu, Wuhan, and Guangzhou. SPSS24.0 and Aoms24.0 were used as multivariate data analysis tools for statistical analysis. The results showed that job autonomy can significantly improve the satisfaction of knowledge employees; however, it cannot affect the satisfaction of knowledge employees through self-efficacy. The findings of the study also revealed that job autonomy has a positive impact on both resource source domain satisfaction and resource acceptance domain satisfaction through work-family enrichment, especially the positive emotions in the resource source domain. Job autonomy improves the self-efficacy of knowledge employees, which, in turn, improves their overall satisfaction through the work-family enrichment path.

Diamond-wire-sawn (DWS) technology has been widely used in the photovoltaic industry. When using the HF/HNO3/H2O acid etching solution for texturing of DWS multi-crystalline silicon(mc-Si), the aid of additive is required to improve the reactivity of the mc-Si surface in the acid texturing solution. It also needs to enhance the nucleation and uniform growth of the texturing surface. This paper proposes a non-metallic additive for DWS mc-Si texturing. Sodium polyacrylate is added to the HF/HNO3/H2O acid etching solution to reduce the reflectance of DWS mc-Si and improve surface morphology. Compared to the textured wafers without additive, the surface of the wafers using this method is uniformly distributed with pits whose size is 0.5 μm×1 μm. And the weighted average reflectance of the textured wafers can be reduced from 33.32% to 23.9% in the wavelength range of 350–1100 nm, with the lowest reflectance of 19.8% reached at 950 nm. It shows a promising application prospect.

APPRENTICE is a tool developed for event generator tuning. It contains a range of conceptual improvements and extensions over the tuning tool Professor. Its core functionality remains the construction of a multivariate analytic surrogate model to computationally expensive Monte-Carlo event generator predictions. The surrogate model is used for numerical optimization in chi-square minimization and likelihood evaluation. Apprentice also introduces algorithms to automate the selection of observable weights to minimize the effect of mis-modeling in the event generators. We illustrate our improvements for the task of MC-generator tuning and limit setting.