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The in-situ synthesized particle reinforced TC4 matrix composites were prepared by powder metallurgy pressureless sintering using polycarbosilane （PCS） as precursor. The thermal compression simulation experiments were conducted on TC4-1PCS （mass fraction of PCS is 1%） composites at 850-1100 ℃ and 0.001-1 s-1 to analyze the stress-strain curves of the composites under different parameters using the Gleeble-3500 thermal simulation testing machine. The effects of deformation parameters on the reinforced phase particles， matrix structure and densification were analyzed by OM， SEM and EBSD methods. The results indicate that the TiC reinforced phase particles with the size of 5-10 μm and large amount of residual pores are observed in the TC4-1PCS composites before hot deformation. The β transition temperature（Tβ） of TC4-1PCS matrix is 1000-1050 ℃. When deformed above Tβ， matrix of composite consists of lamellar quenched martensite， while the matrix turns into duplex microstructure， when deformed below Tβ. The deformation temperature determines the relative density and microstructure types of the composites， while the strain rate affects the phase size in the matrix and residual porosity. The densification of TC4-1PCS composites can be promoted by the increase of deformation temperature and the decrease of strain rate， while the increase of strain rate has obvious effect on the microstructure refinement. The microstructure refinement and densification of TC4-1PCS composites can be achieved by the deformation at 1050 ℃ and 0.1 s-1.

Abstract [Objective] This study aims to accelerate the innovation process of germplasm resources of the seed-used pumpkin (Cucurbita pepo L.) by constructing a mutant library, which is critical for variety selection and improvement of the genetic basis. [Methods] 1.8% ethyl methanesulfonate (EMS) was used to treat ZHL4 seeds of the seed-used pumpkin for 15 h. Phenotypic variation was observed for M1 and M2 populations. Microstructural observation was performed for the mutant line ZHL4-33 of the M2 population. [Results] (1) A total of 242 mutant plants with 45 phenotypic variations were screened in the M2 population, and the type of variations covered all growth periods and plant organs of the mutant plants, with a total mutation frequency of 25.17%. (2) Leaf microstructure showed that the thickness of the fenestrated tissue of the mutant was higher than the wild type, and the arrangement was compact and the traces of the vascular layer were obvious. Stem microstructure showed that the vascular bundles of the mutant were more and densely packed, and the conduit diameter was smaller than the wild type. The pith was well developed, with a small cell gap and an increase in cell numbers. [Conclusion] A mutant library of 425 M2 lines has been constructed, which lays a foundation for functional genomic analysis of the seedused pumpkin as well as the selection and breeding of new varieties of seed-used pumpkin.

ObjectiveTo understand the diarrhea-type virulence genes， the antimicrobial resistance and the molecular typing of emetic Bacillus cereus from the pre-packaged pastries in Taizhou City.MethodsThe Polymerase Chain Reaction （PCR） method was used to identify the vomiting strains of 97 Bacillus cereus strains which were isolated from pre-packaged pastries in Taizhou and detecte whether the vomiting strains carry diarrhea virulence genes. The sensitivity of the vomiting strains to 19 antibiotics was tested by disk diffusion method， and the molecular typing was carried out by multilocus sequence typing technique.ResultsThe results showed that five emetic Bacillus cereus stains were detected of 97 Bacillus cereus strains and divided into ST 26 and ST 1186 two types. Non-hemolytic enterotoxin gene nhe， enterotoxin genes entFM and bceT were the main diarrhea-type virulence genes of the five strains. The five strains were completely resistant to penicillin， ampicillin and cefotaxime. The resistance rates to cotrimoxazole， rifampicin and cefazolin were 80%（4/5）， 40%（2/5） and 20%（1/5）， respectively. The sensitivity rates of amoxicillin， clindamycin and cefoxitin were 40%（2/5）， 80%（4/5） and 80%（4/5）， respectively， and they were completely sensitive to other antibiotics.ConclusionThis study showed that the detection rate of vomiting Bacillus cereus in pre-packaged pastries in Taizhou was 5.2%（5/97）， ST 26 was the main type， there were a variety of diarrhea-type virulence genes in the five vomiting Bacillus cereus strains.

This paper addresses the challenge of managing state constraints in vehicle platoons, including maintaining safe distances and aligning velocities, which are key factors that contribute to performance degradation in platoon control. Traditional platoon control strategies, which rely on a constant time-headway policy, often lead to deteriorated performance and even instability, primarily during dynamic traffic conditions involving vehicle acceleration and deceleration. The underlying issue is the inadequacy of these methods to adapt to variable time-delays and to accurately modulate the spacing and speed among vehicles. To address these challenges, we propose a dynamic adjustment neural network (DANN) based cooperative control scheme. The proposed strategy employs neural networks to continuously learn and adjust to time varying conditions, thus enabling precise control of each vehicle’s state within the platoon. By integrating a DANN into the platoon control system, we ensure that both velocity and inter-vehicular spacing adapt in response to real-time traffic dynamics. The efficacy of our proposed control approach is validated using both Lyapunov stability theory and numeric simulation, which confirms substantial gains in stability and velocity tracking of the vehicle platoon.

ObjectiveThis study aims to conducting a survey on the consumption of nine pilot edible and medicinal substances including Codonopsis pilosula and etc.， and provides a basis for supplementing the database of food safety assessment.MethodsNineteen cities in Guangdong Province were selected as sample sites from 2019 to 2021. Healthy individuals aged 18 years and above who had the habit of eating traditional local food such as soup ingredients were selected as candidate subjects. A food frequency questionnaire was applied for collecting information on the consumption of the nine pilot edible and medicinal substances over the past 12 months.ResultsThis study enrolled a total of 6 233 participants， with 49.2% male and 50.8% female. Among the study population， the consumption rates of the nine pilot edible and and medicinal substances ranged from 0.8% to 55.3%， The consumption rates from high to low are as follows： Codonopsis pilosula（Franch.）Nannf.（55.3%）， Astragalus membranaceus （Fisch.） Bge.var.mongholicus （Bge.） Hsiao （47.4%）， Panax quinquefolium L. （41.5%）， Ganoderma lucidum （Leyss. Ex Fr.）Karst. （dry） （30%）， Dendrobium officinale Kimura et Migo （24.1%）， Gastrodia elata B1. （16%）， Dendrobium officinale Kimura et Migo （wet） （4.8%）， Cistanche deserticola Y.C.Ma （2.6%）， Eucommia ulmoides Oliv. （1.3%）， Cornus officinalis Sieb.et Zucc. （0.8%）. Among the consumer population， the primary mode of consumption for the nine pilot edible and medicinal substances is indirect， accounting for 82.6% to 95.1%. The average monthly consumption frequency ranges from 0.8 to 2.2 times per month. The mean consumption quantity per occasion for the selected dried food-medicinal substances ranges from 7.4 to 11.9 grams， However， the mean consumption quantity per occasion for Dendrobium officinale Kimura et Migo （wet） is 41.9 grams.ConclusionThere are significant differences in the consumption rates of the nine pilot edible and medicinal substances among adults in the 19 municipalities of Guangdong province. The consumption quantity per occasion is high in some substances such as Codonopsis pilosula（Franch.）Nannf.， Astragalus membranaceus （Fisch.） Bge.var.mongholicus （Bge.） Hsiao. It is recommended to further scientifically evaluate the usage and dosage of these food-medicinal substances and make public education on the correct consumption of food for residents.

A semiconductor nanomaterial was prepared and its degradation efficiency on zearalenone (ZEN) was investigated. The photocatalytic material, graphitic carbon nitride (g-C3N4), was synthesized by the conventional thermal cracking method, and its structure was analyzed by (X-ray diffraction) XRD, Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The photocatalytic degradation experiments showed that g-C3N4 induced photocatalytic effects under ultraviolet light (254 nm, including 185 nm), releasing reactive oxygen radicals to degrade ZEN. The experimental conditions for the photocatalytic degradation of ZEN were explored. The results showed that the degradation rate of ZEN was 96.0% when the mass of the photocatalytic material , the wavelength of the UV lamp, the initial concentration and the irradiation time were 20 mg, 254 nm (50 W), 0.5 μg/mL and 60 min, respectively. Meanwhile, the experimental conditions for the photocatalytic degradation of ZEN powder samples were optimized. The results showed that the degradation rate of ZEN was 80.0% when the mass of the photocatalytic material, the wavelength of the UV lamp, and the irradiation time were 800 mg, 254 nm (50 W) and 50 min, respectively. The results could provide a theoretical reference and practical basis for the photocatalytic degradation of ZEN.

Thyroid-associated ophthalmopathy(TAO)is a rare organ-specific autoimmune disease with an unclear pathogenesis. At present, the treatment still relies mainly on glucocorticoids and traditional immunosuppressants. However, some patients respond poorly to these drugs and experience treatment-related adverse reactions, highlighting the urgent need for novel drugs for TAO treatment. In recent years, with the deepening of research on the pathogenesis of TAO, a multitude of biologics targeting specific targets have emerged. Among them, teprotumumab, which targets the insulin-like growth factor-I receptor(IGF-IR), has been approved by the Food and Drug Administration for the treatment of TAO, and several other biologics are currently in clinical trials. This review provides the latest reference for the clinical prevention, treatment, and research of TAO by summarizing the current clinical research status of biologics targeting IGF-IR, neonatal Fc receptor(FcRn), thyroid-stimulating hormone receptor(TSHR), B cells, cytokines, and other biological agents in TAO and analyzing their impact on clinical treatment and future research trends.

[Objective] The study aims to explore the effects of different foliar fertilizers on the photosyn thetic characteristics, fruit quality, and yield of greenhouse strawberries, and to screen suitable foliar fer tilizers. [Methods] The strawberry variety “Monterey” planted in the greenhouse of Moujiatai Strawberry in Xigu District of Lanzhou City, Gansu Province was used as test materials. Under sufficient fertilization, five types of foliar fertilizers, including classic 750×liquid (T1), Huapu 1 500×liquid (T2), and Runliang 750×liquid (T3), 500×Adela fruit (T4), and 1500×potassium dihydrogen phosphate (T5), were ap plied topdressing at four growth stages, with water as control. The photosynthetic parameters, fruit qual ity, biomass, and yield were measured. [Results] (1) After spraying different foliar fertilizers, the bio mass and chlorophyll content of strawberry plants were significantly increased, and the net photosynthetic rate and transpiration rate of leaves were significantly increased, while the intercellular carbon dioxide con centration was higher than that of CK. (2) For fruit quality, T2 and T3 treatments significantly improved the strawberry fruit shape index, while T1 and T5 treatments significantly increased fruit volume and weight. Among them, the average single fruit mass of T1 was increased by 35.04% compared with CK, while the transverse and longitudinal diameters of T5 fruits were increased by 49.39% and 40.64%, re spectively. (3) The quality of strawberry fruits was effectively improved by various foliar fertilizer treat ments, and T1 treatment was the best. The content of vitamin C, soluble solids, soluble proteins, reduc ing sugars, and soluble sugars were increased by 30.84%, 33.5%, 38.5%, 27.9%, and 36.2%, respec tively, compared with CK. The content of titratable acid was the lowest, and the sugar acid ratio was the highest, with significant difference compared with CK. (4) Spraying appropriate fertilizer significantly in creased plant biomass, max single fruit weight, and fruit yield; treatment T1 had the highest yield, an in crease of 13.65% compared with CK. [Conclusion] The application of appropriate foliar fertilizer effective ly improves the nutritional growth status of strawberries, enhances the quality of strawberry fruits, and significantly increases yield. The comprehensive effect is best when applying classic 750×liquid.

Intelligent control systems can effectively assist in the construction and management of laboratory animal facilities, improving operational efficiency, ensuring the reliability of animal experimental results, and significantly saving human resources. The intelligent control system for laboratory animal facilities at Shenzhen Institute for Drug Control was completed in April 2021. It includes an intelligent management platform and an information management system for animal laboratories. The intelligent management platform regulates room environment parameters such as temperature, humidity, and pressure through building equipment management system, controlling devices such as the Venturi valve, electric air valve, electric water valve, and steam humidification valve. At the same time, various environmental parameters are monitored online through the environmental monitoring system. The laboratory’s intelligence is further enhanced by systems such as automatic lighting control, full HD video monitoring, automatic access control and door system, independent ventilation and feeding, automatic cleaning, automatic exhaust gas treatment, centralized gas supply, and real-time instrument parameter monitoring. The information management system for animal laboratories integrates inspection, instrument and equipment, personnel, documents, standard substances, reagents, inspection standards, books, records, scientific research management, relevant applications, quality management, and query statistics. For animal experimentation, a management module has been developed to achieve a comprehensive digitization of animal management. Furthermore, real-time collection and recording of data such as balance calibration, sample quality, and animal weight are facilitated through electronic experimental recording. In summary, the Animal Laboratory of Shenzhen Institute for Drug Control has extensively utilized intelligent systems to achieve real-time online control and monitoring, improve efficiency, ensure high-quality facility operation, and meet standard requirements. Smooth execution of all inspection and research activities has been achieved over the past three years. This paper provides insights into the construction, management, and operation of laboratory animal facilities at Shenzhen Institute for Drug Control, offering guidance for the implementation of intelligent control in similar facilities across China.

Polymer-based transition metal complexes have attracted much attention in many fields of application. In this article, a fluorescent polymer-based transition metal complex was prepared by bonding the transition metal complex with the polymer. First, Schiff base salicylaldehyde ethanolamine (HL) as a ligand was prepared by the reaction of salicylaldehyde with ethanolamine. Then, salicylaldehyde glycolamine Zn(ii) transition metal complexes (ZnL2) were synthesized with HL and Zn2+ as the central ion. Finally, a blue fluorescent waterborne-based polyurethane Zn(ii) complex (ZnL2-WPU) with an antibacterial function was prepared with ZnL2 as a chain extender by modified acetone method. The characteristics of fluorescence, heat stability, and bacteriostasis were characterized. Compared with ZnL2, the UV–vis absorption peak of ZnL2-WPU shows a blue shift of about 20 nm. ZnL2-WPU has a strong blue fluorescence emission at 450 nm, and the intensity increases significantly with ZnL2 content. Surprisingly, the fluorescence lifetime of ZnL2-WPU is obviously increased, reaching more than one time that of ZnL2. Interestingly, the antibacterial efficiency of ZnL2-WPU against E. coli reached an incredible 99%. More importantly, ZnL2-WPU uses water as the dispersing medium, which is more environmentally friendly.

In recent years, according to incomplete statistics, acute respiratory infections caused by viruses account for approximately 80% of all respiratory diseases triggered by pathogenic microorganisms, making these the diseases with the highest incidence rates worldwide. Respiratory viruses, such as the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), SARS-CoV-2, influenza A virus and respiratory syncytial virus, pose a threat to public health, yet no specific drugs have been developed to combat these viruses effectively. Due to its synergistic multi-component and multi-target actions, Traditional Chinese Medicine (TCM) has unique advantages in the prevention, treatment, and rehabilitation of respiratory viral diseases. In recent years, many clinicians and researchers have conducted extensive studies and explorations into the use of TCM for treating respiratory viral diseases, leading to a clearer understanding of its clinical efficacy and therapeutic mechanisms. This article reviews the clinical efficacy and mechanisms of action of TCM in treating respiratory viral diseases, providing strong evidence for its enhanced treatment capabilities and promoting the development and potential application of TCM in the field of respiratory viral diseases.

This study aims to investigate the significance of using the Toumai robotic platform for the resection of inferior vena cava (IVC) hemangiomas. Our objective is to provide information on the potential benefits of this innovative approach in managing these uncommon vascular tumors and to contribute to the growing body of knowledge in the field of surgical oncology and vascular surgery. A 37-year-old female patient with an incidental finding of a right retroperitoneal mass underwent contrast-enhanced magnetic resonance imaging, which confirmed the diagnosis of an IVC hemangioma. Due to the rarity and complexity of this vascular tumor, the medical team opted for a novel approach using the Toumai robotic surgical platform (Shanghai MicroPort MedBot Group Co, Ltd). Under general anesthesia, the patient was placed in the left lateral decubitus position, and the robotic arms were controlled remotely through the Toumai platform. A transperitoneal approach was adopted, and the surgeon meticulously resected the tumor while preserving the integrity of the IVC. The use of the Toumai robotic platform facilitated precise tumor resection, minimizing the risk of damage to surrounding structures. The minimally invasive nature of the robotic surgery contributed to a reduced incidence of postoperative complications and accelerated patient recovery. Furthermore, the remote fifth-generation mobile network surgical capabilities of the Toumai platform allow for expert care to be provided to patients despite geographic barriers. The robotic-assisted surgical approach using the Toumai platform demonstrates its potential benefits in managing rare and complex vascular tumors such as IVC hemangiomas. Robotic technology has the potential to revolutionize the field of surgical oncology and vascular surgery, leading to improved patient outcomes and healthcare delivery. However, more extensive clinical studies and larger case series are needed to validate the long-term safety and efficacy of this innovative surgical approach. Continued research and collaboration between clinicians and robotic technology experts are essential to fully realize the potential of robotic-assisted surgery for the benefit of patients with rare and challenging medical conditions.

Objective To analyze the development method of existing clinical practice guidelines in primary care to inform the future development of high-quality clinical practice guidelines in primary care. Methods Chinese and English databases and guideline websites were systematically searched for clinical practice guidelines in primary care published from January 1, 2019 to December 31, 2021, with additional searches conducted by reference tracing and Google Scholar. After screening was completed, basic information on included studies and information related to the development methodology was extracted and analyzed. Results Twenty primary guidelines published between 2019 and 2021 were finally included, 95% of which originated from developed countries. The most common developing institutions were universities/research institutes (8, 40%), the disciplines with the most attention were nursing (4, 20%), infectious diseases (3, 15%), and gastroenterology (3, 15%), and the areas of the guidelines were mainly focused on management (4, 20%), treatment (4, 20%) and nursing (3, 15%).None of the guidelines were registered, and 11 (55%) guidelines used evidence grading and strength of recommendation criteria. Mean reporting on the 18 topics in the Guideline 2.0 inventory was 40%, with relatively good reporting on the topics of guideline panelists (85%), conflict of interest considerations (75%), and evidence retrieval and incorporation (75%), and low reporting on the topics of organizing-budgeting-planning-training (15%), and summarizing the evidence and considering other information (15%). Conclusions The number of clinical practice guidelines in primary care that explicitly report on the methodology and process of development is low, and there is insufficient consideration of the relevant topics in the Guideline 2.0 checklist. It is recommended that future research focus on standardizing the reporting of clinical practice guidelines in primary care and developing the methodological guidebook for guideline development.

Evidence synthesis is the synthesis of existing research using a systematic and appropriate method. Various types of evidence synthesis are currently in use. Correctly choosing and applying the best evidence synthesis method is crucial for researchers, policymakers, and stakeholders. Thus, based on summarizing the current situation, progress, and classification of evidence synthesis methods, we introduced the definition and characteristics of common evidence synthesis methods and problems in their application, and presented thoughts and suggestions for the problems in the application of evidence synthesis methods.

In this study, metal–organic nanofibers (MONFs) and reduced graphite oxide (rGO) nanocomposite were used to modify the surface of glassy carbon electrode, and the electrochemical sensor was applied to the differential pulse voltammetry determination of hypoxanthine, the oxidation intermediate of human purine degradation metabolism. The preparation of MONFs/rGO nanocomposite is simple, efficient, and environmentally friendly. The morphology and structure of MONFs/rGO nanocomposite were characterized by field emission scanning electron microscopy and X-ray diffraction. The results show that the improved sensor has a significant increase in current density, with linear ranges of 0.1–10 and 20–100 μM. Detection limit 0.01 μM (S/N = 3). Under the optimized conditions, the improved sensor shows very good stability, selectivity, and improved accuracy.

The purpose of this study was to examine the characteristics of proximal and distal gastroesophageal reflux in patients with gastroesophageal reflux disorder and analyze their clinical symptoms. A total of 67 patients with typical esophageal symptoms were selected for this study. All participants completed the reflux disease questionnaire and a questionnaire survey of extraesophageal symptoms. Diagnosis was made using a 24-h impedance-pH detection and proton pump inhibitor. The results showed that the proximal reflux group had a higher number of acid reflux episodes compared to the distal reflux group (P < 0.05). Similarly, the proximal reflux group also had a higher number of gas reflux episodes compared to the distal reflux group (P < 0.05). On the other hand, the distal reflux group had a higher number of mixed reflux episodes compared to the proximal reflux group (P < 0.05). These differences were statistically significant. This study revealed that acid reflux and gas reflux were more predominant in the proximal reflux group, while mixed reflux was more predominant in the distal reflux group.

ObjectiveThis study aimed to understand the epidemic characteristics of Diarrheagenic Escherichia coli （DEC） in domestic meat products.MethodsThe molecular characteristics of 86 strains of DEC were analyzed by whole genome sequencing technology， and the dominant pathological types， STs， and serotypes were identified. The phylogenetic relationship between DEC strains derived from meat products in China was determined through whole genome single nucleotide polymorphism.ResultsThe results showed that the pathological types of DEC in meat products were mainly EAEC. Eighty-six DEC strains were divided into eight virulence genotypes， including 48 ST types （including six new ST types）， and 55 O：H serotypes. ST11 and CC10 were the predominant ST and clonal complex， respectively. O157， O15， and O6 were the predominant serogroups. DEC strains exhibited high genetic heterogeneity， the same pathological type had low homology， and they were in different evolutionary branches.ConclusionThere is no correspondence between DEC pathological types and ST types and serotypes. The pathological types cannot be directly judged by monitoring ST types and serotypes， but the most numerous ST types and serotypes reveal the main hazard characteristics and prevention of DEC. The determination method of DEC can be supplemented and optimized according to the results of whole genome sequencing， which can provide data support for the traceability and epidemiological investigation of DEC in the future.

Geomorphic features contribute to variations in regional climate, soil, and other ecological environment factors, which are also an important basis for the biodiversity and evolution of ecosystems. Studying geomorphic features in nature reserves provides a geomorphic basis for resource and environmental management and protecting ecology and biodiversity in these areas. This study revealed the terrain variation, geomorphic patterns, and developmental evolution of the Wumeng Mountain National Nature Reserve by combining quantitative and qualitative methods by investigating geomorphic features and advanced land observing satellite (ALOS) digital elevation model (DEM) terrain analysis. The results indicated that: (1) the reserve elevation was 980~2,454.7 m, with an average of 1,859.9 m, and the undulation was 390~2,104.7 m, with an average of 1,392.1 m. The basic geomorphic types of the reserve were mainly large undulating middle mountains (57.45), large undulating subalpine mountains (30.66%), and medium undulating mesas (11.17%), with an average slope of 21.1°. The gentle slopes (34.83%) had the highest proportion, followed by slopes (31.21%) and gently steep slopes (17.57%). The area of each aspect was the highest on half shady slopes (27.92%) and the least on sunny slopes (21.70%); (2) the reserve was located at the top part of the mountain, with the slope and undulation in the middle of each area being low and the surrounding area being high. From Sanjiangkou to Chaotianma and Haiziping, the average values of geomorphic parameters, such as elevation, undulation, slope, and hypsometric integral, decreased gradually, and there was a trend of progressive reduction in planation surfaces; (3) the terrain profile indicated seven planation surface levels around the reserve. Among them, levels Ⅰ-Ⅲ were the summit surfaces and residual planation surfaces, level Ⅶ was the Jinsha River erosional terrace surface, and levels Ⅳ-VI were the planation surfaces, corresponding to the three tectonic cycles in the area; (4) the hypsometric integral of the reserve ranged from 0.197 to 0.815, with an average of 0.503, and more than 80% of the area was in adulthood of geomorphic evolution, indicating that the surface erosion dynamics of the area was strong, and there was a greater ecological risk with enhanced human activities. Consequently, future conservation planning should consider differences in the surface dynamics of areas with various basic geomorphic types. Geomorphic features form an important part of the natural ecosystems of forest reserves. The unique and rich geomorphic structure enables the conservation and development of biodiversity along with the elements of the ecological environment on which it depends. Geomorphic diversity supports the analysis of the biological formation, evolution, and persistence mechanisms in reserves and provides geomorphic evidence for studying neotectonic activity in the area.

Background Clinical practice guidelines in primary care are important tools to guide primary care practice, but they are found with relatively low quality and unstandardized reporting on the whole. Objective To develop the reporting guideline for clinical practice guidelines in primary care based on the Reporting Items for practice Guidelines in HealThcare (RIGHT) . Methods According to the reporting methods recommended by EQUATOR Network, an item development group and an expert consensus group were established to develop reporting guideline for clinical practice guidelines in primary care. Content analysis, brainstorming, and topic analysis were used in developing the initial items. The Delphi survey was conducted to determine the final items. Results Thirteen items (five were modified and eight were added) were developed initially, covering five domains: guideline title, health issues, intended setting, recommendations and suggestions for future research. Then the initial item checklist was revised to a final version consisting of 15 items according to the results of two rounds of Delphi survey (with 100% response rates from 22 experts) conducted from December 2 to December 26, 2022. Conclusion This reporting checklist will provide a detailed guide for developers of guidelines in primary care, which will further improve the reporting quality of guidelines in primary care, especially that of those in China.

Background The clinical practice guidelines in general practice can improve healthcare quality in primary health care, however, no study has yet systematically investigated the current status and quality of the guidelines in China. Objective To investigate the current status and influencing factors of quality of the guidelines in China. Methods We searched China National Knowledge Infrastructure, Wanfang Data Knowledge Service Platform, Chinese biomedical literature database, and CQVIP website, and included the published guidelines in China. We analyzed the basic characteristics and used RIGHT to evaluate the reporting quality and AGREE-China for methodological quality. Results A total of 150 guidelines were included, mainly published from 2019 to 2021. Most of the guidelines〔108 (72.0%) 〕focused on the diagnosis and treatment of diseases. The top three specialties were cardiovascular disease〔40 (26.7%) 〕, gastroenterology〔31 (20.7%) 〕, and clinical pharmacy〔27 (18.0%) 〕. The main development institutions were the Chinese Medical Association and its branches〔123 (82.0%) 〕, the editorial committee of the Chinese Journal of General Practitioners〔119 (79.3%) 〕, and the Chinese Medical Journals Publishing House〔116 (77.3%) 〕. In terms of reporting quality, the average reporting rate of RIGHT was 23.6% (11.4%-42.9%) ; the reporting rate of basic information (59.8%) was higher, and that of evidence (0.3%) was the lowest. As for methodological quality, the average AGREE-China score was 23.4 (12.0-40.0) ; the reporting rates of conflicts of interest (63.0%) and availability/feasibility (53.0%) were higher, and that of economics (7.0%) was the lowest. Conclusion The number of Chinese clinical practice guidelines in general practice has increased rapidly in the past five years, which has played an important role in promoting the quality of primary health care. In the future, it is necessary to further develop the guidelines in different specialties and diseases and accelerate the methodology of development and reporting of the guidelines.

In recent years， stable isotope technology has been widely used in product quality control and authenticity identification. This paper summarizes the research progress of this technology in food safety traceability and authenticity identification of various foods in recent years， looks forward to its application in China， and hope to provide reference for the administrative authoritives.

Objective To explore the medication for lung cancer treatment at Hubei Cancer Hospital. Methods The electronic cases of 3 234 hospitalized lung cancer patients in Hubei Cancer Hospital were collected. The medication of the Peiyuan Guben method in treating lung cancer was analyzed with cluster and association analyses by the Apriori association rule algorithm in IBM SPSS modeler 18.0 and Qihuang data AI workstation software system. Results In the 11 293 pieces of traditional Chinese medicine used in 3 234 patients with lung cancer, the core prescription of 16 core drugs were Astragalus, Coix seed, Poria, Coke hawthorn, Ligustrum lucidum, Wolfberry, Bran fried atractylodes, Mushroom stem, Bran fried citrus husk, Bittersweet herb, Centipede, Rhodiola, Semiaquilegia adoxoides, Seaweed, Prunella and Pseudobulbus cremastrae seu pleiones. Conclusion "Peiyuan Guben" is an effective method of traditional Chinese medicine in treating lung cancer. Traditional Chinese medicine with the effects of Yiqi Jianpi and Bushen Yijing has an evident curative effect in treating lung cancer and can improve patients' quality of life.

Background: To explore the difference and diagnostic value evaluation of serum tumor markers in different clinical stages of elderly non-small cell lung cancer (NSCLC) patients. Methods: Select 100 elderly NSCLC patients admitted to our hospital from June 2018 to June 2021, collect the general data, pathology data and imaging data of the patients, and the patients were divided into I-IV clinical stages according to the International Union Against Cancer (UICC) 8th edition lung cancer TNM staging standard. Detect the subjects' peripheral serum tumor markers, serum carcinoembryonic antigen (CEA), cytokeratin fragment 21-1 (CYFRA21-1), squamous cell carcinoma-associated antigen (SCCA), carbohydrate antigen 125 (CA125) and sugar Class antigen 199 (CA199). Compare the differences of serum CEA, CYFRA21-1, SCCA, CA125, CA199 levels in different clinical stages of elderly NSCLC patients, and the diagnostic value of the above indicators for elderly NSCLC patients was analyzed by receiver operating characteristic curve (ROC curve) and area under the curve (AUC). Results: The smoking history, differentiation degree, lymph node metastasis and tumor diameter of elderly NSCLC patients with different clinical stages are significantly different; with the increase of NSCLC disease progression, the levels of serum CEA, CYFRA21-1, SCCA, CA125, and CA199 continue to increase, in serum. There is a significant correlation between the markers; serum CEA, CYFRA21-1, SCCA, CA125, CA199 have diagnostic value for the clinical staging of elderly NSCLC patients. Conclusion: The elevation of serum CEA, CYFRA21-1, SCCA, CA125 and CA199 in elderly NSCLC patients is positively correlated with the degree of tumor malignancy. The detection of the above indicators is of great value for the early diagnosis and disease monitoring of elderly NSCLC.

The Shanwei Special Cooperation Zone (SSCZ) in Shenzhen is the main distribution area of the Lianhuashan Fault Zone. Two large ductile shear zones pass through the SSCZ. The ductile shear zone of the SSCZ is henceforth referred to as "the zone". Severe plastic progressive deformation, stretching, and compression occurred in the late Jurassic-Neogene period in the zone, because of which the structure and properties of the rock mass underwent significant changes. There are three levels of structural planes in the zone: brittle faults, schistosity planes, and joints in the rock mass. The ductile shearing action changes the rock mass structure, leading to poor integrity. According to existing data, the compressive strength of the rock in the zone is equivalent to 0.31-0.86 of the original rock, indicating that the strength of the rock is obviously attenuated by ductile shear action. Using ground investigation and remote sensing, we found 1,614 topsoil slips in the zone. The density of topsoil slips in the hillside area (16.2 topsoil slips per km2) was much higher than that outside the zone (1.3 topsoil slips per km2), indicating that the slopes in the zone are more prone to shallow damage. To study the geological risk zonation of geological hazards in the SSCZ, it is necessary to determine the characteristics and distribution rules of topsoil slips in the zone, analyze its causal mechanism, and predict its development trend.ⅰ) Ground surveys and remote sensing showed that monocase topsoil slip is small in the zone and sliding mainly occurs along the bedrock surface. Sliding masses are mainly solid and completely weathered rock. ⅱ) The frequency of geological disasters under the action of certain factors in the study area is divided by the frequency of geological disasters in the area. The natural logarithm of the divisor is taken as the information value to evaluate the relationship between geological disasters and various factors. The results show that the density of topsoil slips is positively correlated with the degree of ductile deformation (strong→medium→weak), distance from brittle fracture (near→far), and topographic slope (high→low). Moreover, the distribution density is strongly related to the geomorphic unit, slope direction, original rock type, and slope type. ⅲ) The above analyses are combined with regional, geological, exploration, meteorological, and other data. Results from this combination show that topsoil slips in the zone are produced under weak background conditions formed by the influence of multi-stage tectonic action dominated by ductile shear forces, the slow rise of the hillside area, and the induction of typhoons and rainstorms. Their development and changes are characterized by migration, self-healing, and expansion. ⅳ) Most of the current topsoil slips occurred during Super Typhoon Mujigae in 2015, after which the region experienced several rainfall events with intensities higher than those of Typhoon Mujigae, yet the number of topsoil slips did not increase significantly. This shows that after a general shallow sliding event, the sensitivity of the slope to shallow damage decreased, and generation took a long time before entering the next outbreak period.

This study was conducted to gain insight into the distribution characteristics of Soil Organic Matter (SOM) and Soil Integrated Fertility (SIF) in various mountain soil zones and several typical land use types (secondary forest, banana plantation, and rubber plantation) on different altitude gradients in the northern tropics and the Butterfly Valley area in Jinping County, Yunnan Province, China. The surface (0-20 cm) and subsurface (20-40 cm) layers are distinguished. SOM, pH, total N, total P, total K, and available N, P, and K were measured. The SIF was evaluated using the modified Nemorow index method, and the key limiting factors were analyzed. First, the results show that the SOM content ranges from 21.91 to 120.67 g/kg for the surface layer and 16.38 to 101.88 g/kg for the subsurface layer, with the mean SOM of the surface layer (75.32 g/kg) greater than that of the subsurface layer (51.57 g/kg, P > 0.05). In terms of SIF, that of the surface layer was 1.15-1.59 and that of the subsurface layer was 0.94-1.44, with the mean SIF of the surface layer (1.35) greater than that of the subsurface layer (1.16). This shows that there is a significant positive correlation between the altitude gradient and SOM as well as SIF. Ranking of SOM and SIF for the surface layer (P < 0.05). Second, the SOM for the surface layer of the secondary forest, banana plantation, and rubber plantation were 26.61, 13.48, and 11.91 g/kg, respectively, while the SOM for the subsurface layer of these three typical land use types are 19.28, 9.61, and 8.91 g/kg, respectively. With regard to SIF, they were 1.48, 1.33, and 1.21 for the surface layer, and 1.17, 1.10, and 0.94, respectively. Notably, both in the surface and subsurface layers, the SOM and SIF were largest in the secondary forest, followed by the banana plantation, and then the rubber plantation. The SOM of the secondary forest was significantly greater than that of the banana and rubber plantations (P < 0.05), whereas there was no significant difference among the SIF of these three land use types. Finally, as the altitude increased, the factors limiting the SIF change from available N and available P to available P and available K, and the effect of pH gradually strengthened. The factors that maintain and limit the SIF of different land use types at low altitudes are generally consistent with natural soils at the same altitude. Therefore, in the process of future mountain development and utilization, it is important for low-altitude areas to supply nitrogen and phosphate to the soil. In medium- and high-altitude areas, supplementation with phosphate and potash is useful, and a suitable pH helps the soil release nutrients and promotes plant uptake and utilization.

For the problem that how the grouting reinforcement of a single penetrated structural plane affects the bearing capacity of surrounding rock, an approach of rock samples preparation with single penetrated fracture surface is proposed firstly, and the rock samples are injected with ultrafine cement and epoxy resin, respectively. Then, the strength and deformation characteristics of the consolidated body under uniaxial (triaxial) compression are investigated with the RMT-150C experimental system. Finally, the micro consolidation mechanism of structural plane grouting is revealed using theoretical analysis and electron microscope scanning. It is found that the fracture surface generated under the triaxial loading-unloading condition is closer to the engineering practice and meets the experimental requirements. The stress−strain characteristic curves of the specimens injected with ultrafine cement show phased deformation characteristics, and the curves of the specimens injected with epoxy resin are relatively smooth. For the strength characteristics, the influence of confining pressure is significantly larger than that of grouting material selection. Grouting reinforcement can improve the residual strength of rock mass. With the increase of confining pressure, the increase of improvement coefficient of residual strength is less obvious, and the peak strength of the consolidated body is closer to that of intact rock. For the failure characteristics, the main fracture surface of the specimen injected with ultrafine cement shears and slips along the original fracture surface, and the main fracture surface of the specimen injected with epoxy resin is a new penetrated fracture surface. Finally, based on the Mohr-Coulomb criterion, the relationship between the bonding force of grouting materials and the strength improvement coefficient of structural plane is established. It is found that the strength improvement coefficient of structural plane has a linear relationship with the bonding force of grout. The comparison between the calculation and test results shows that the formula is reasonable and can provide a reference for the optimization of deep surrounding rock support.

With the increasing demand for filling engineering construction in the Loess Plateau, the engineering problems related to saturated loess caused by rainfall and irrigation are also increasingly prominent. This work studied the shear characteristics of saturated compacted loess by experiments, and qualitatively and quantitatively explained the meso-mechanism of the effect of compaction degree on the shear characteristics combined with the meso-pore characteristic parameters. The study shows that the stress–strain curve of saturated loess under 80 and 85% compaction degree is strain softening, it is strain weak softening under 90% compaction degree, and strain hardening under 95% compaction degree. The failure strength and pore water pressure have a good fitting linear relationship with compaction degree, and the fitting correlation coefficients are more than 0.97. Under 95% compaction degree, the pore water pressure increases with the development of axial strain, then decreases after the peak, and may appear negative. With the increase in compaction degree, the micro and small pore content of the saturated loess soil increases, the medium, large and super large pore content decreases, the proportion of pore area decreases, the pore shape becomes uniform and smooth, the complexity of pore shape decreases, the pore arrangement tends to be regular and neat, and the directionality increases. The research results can provide references for engineering design, construction, and numerical calculation of filling engineering in loess area.

BACKGROUND Detrital zircon U-Pb geochronology is an important tool for identifying sedimentary provenance and determining the maximum depositional age. The numbers of grains for detrital zircon provenance investigations using laser-ablation inductively coupled-plasma mass spectrometer (LA-ICP-MS) typically range from 60 to 120. In this range, age components are commonly not identified from the sample aliquot. In order to improve the reliability of provenance investigation, analysis of more grains (n≥300) or even the large-n aliquot with more than 1000 grains (n > 1000) are required. The emergence of large-n detrital zircon U-Pb geochronology is challenging the methods of data measurement, reduction and evaluation. OBJECTIVES To summarize the progress of measurement, data reduction and data evaluation of large-n detrital zircon U-Pb geochronology. METHODS By summarizing the method innovation of domestic and foreign literature. RESULTS Firstly, each measurement requires rapid acquisition of U and Pb isotope signals, which can be conducted by improving the transmission efficiency of aerosol. The "flat" signal acquisition time can be shortened or transformed to a "peak" signal mode for rapid measurement. Secondly, large-n data require efficient data reduction protocol or powerful software (e.g. iolite) to improve visualization and reduce the variability between inter-laboratory comparisons. For U-Pb data processing flow, several optimized methods are introduced for fractionation correction and propagating uncertainty. In addition, total integrated counts and linear regression correction are introduced to specially process "peak" signals. Thirdly, the new calculation method of U-Pb and Pb-Pb age discordance, such as using Aitchison concordia distance, makes data filtering more reasonable. Based on recent research progress, the future of automation and standardization of large-n detrital zircon U-Pb geochronology is discussed and advice on the selection of instruments and reduction software is provided. CONCLUSIONS In the future, the development of large-n detrital zircon U-Pb geochronology has great prospects, and will play a greater role in the study of provenance tracing and stratigraphic dating.

Objective To broaden the differential diagnosis thinking of diffuse cystic lung diseases and multiple nodular lung disease, and to deepen the understanding of clinical phenotypes of tuberous sclerosis complex (TSC). Methods A case of TSC with complaint of diffuse lung diseases detected during a routine check-up was presented and relevant literatures were reviewed. Results Further examination confirmed multisystem involvement in the patient including heart, kidney, liver and bone, mimicking metabolic malignant tumor. However, percutaneous renal mass biopsy showed angiomyolipoma, which suggested TSC combined with “Confetti” skin lesions found on physical examination. Peripheral blood whole exome sequencing confirmed the diagnosis of TSC. The pulmonary lesions were improved after treatment with sirolimus. Conclusions High clinical awareness of TSC is essential in the patients with diffuse cystic lung diseases and multiple nodular lung diseases, especially in women. In-depth knowledge of multisystem manifestation in TSC is critical for early diagnosis and treatment of this rare disease.

ObjectiveTo provide data basis for improving the accuracy of food safety risk assessment in China， the cooking coefficients of five toxic metals in marine fish was studied.MethodsBased on the consumption of 19 kinds of marine fish in Guangdong province， samples were divided into steamed， fried， roasted group and a control group （raw fish）. The concentrations of the total mercury， methyl mercury， arsenic， cadmium and lead were determined before and after the cooking process， and the cooking coefficients of five toxic metals were calculated.ResultsThe concentrations of total mercury， methyl mercury， total arsenic and cadmium in fried and roasted fish were significantly higher than those in control group （P

BackgroundThere is limited data on antibiotic treatment in hospitalized neonates in low- and middle-income countries (LMICs). We aimed to describe patterns of antibiotic use, pathogens, and clinical outcomes, and to develop a severity score predicting mortality in neonatal sepsis to inform future clinical trial design.Methods and findingsHospitalized infants ConclusionAntibiotic regimens used in neonatal sepsis commonly diverge from WHO guidelines, and trials of novel empiric regimens are urgently needed in the context of increasing antimicrobial resistance (AMR). The baseline NeoSep Severity Score identifies high mortality risk criteria for trial entry, while the NeoSep Recovery Score can help guide decisions on regimen change. NeoOBS data informed the NeoSep1 antibiotic trial (ISRCTN48721236), which aims to identify novel first- and second-line empiric antibiotic regimens for neonatal sepsis.Trial registrationClinicalTrials.gov, (NCT03721302).

Abstract Background Fetal macrosomia is associated with an increased risk of several maternal and newborn complications. Antenatal predication of fetal macrosomia remains challenging. We aimed to develop a nomogram model for the prediction of macrosomia using real-world clinical data to improve the sensitivity and specificity of macrosomia prediction. Methods In the present study, we performed a retrospective, observational study based on 13,403 medical records of pregnant women who delivered singleton infants at a tertiary hospital in Shanghai from 1 January 2018 through 31 December 2019. We split the original dataset into a training set (n = 9382) and a validation set (n = 4021) at a 7:3 ratio to generate and validate our model. The candidate variables, including maternal characteristics, laboratory tests, and sonographic parameters were compared between the two groups. A univariate and multivariate logistic regression was carried out to explore the independent risk factors for macrosomia in pregnant women. Thus, the regression model was adopted to establish a nomogram to predict the risk of macrosomia. Nomogram performance was determined by discrimination and calibration metrics. All the statistical analysis was analyzed using R software. Results We compared the differences between the macrosomic and non-macrosomic groups within the training set and found 16 independent risk factors for macrosomia (P

In order to meet the needs of rapid quantitative detection of fumonisin B1 (FB1) in grains, this paper takes fumonisin B1 as the research object, and creates a rapid quantitative detection card of FB1 on the basis of time-resolved fluorescent nanospheres, with no need to adjust the PH of samples before detection. The detection performance of the detection card in grains (rice, corn, wheat) was analyzed and compared with enzyme-linked immunosorbent assay kit and high performance liquid chromatography. The LOD for different samples was in the range of 35.37~37.17 μg/kg, and the LOQ was 117.9~123.9 μg/kg, which meant good sensitivity; the linear range was 250~6 000 μg/kg, while the correlation coefficient R2 was in the range of 0.997 2~0.999 5; the spiking recoveries at 6 spiked levels in different samples were in the range of 83.38%~114.66%, with the coefficient of variation (CV) less than 15%, which meant good accuracy and repeatability. When the national standard liquid chromatography and FB1 fluorescence quantitative rapid detection card simultaneously detects different samples contaminated by FB1, the coincidence of the detection results was in the ranger of 92.53%~106.26%, with the CV less than 10.37%. The cross-reaction rate of other five common mycotoxins was less than 5%. Meanwhile, the difference between the spiked concentration and their average detected concentration was extremely significant, indicating good specificity. Hence, the FB1 fluorescence quantitative detection card prepared in this study could meet the needs of on-site rapid quantitative detection of fumonisin B1 in grains.

Background and objectivePrevious studies have shown that patients with psoriasis are at higher risk of developing chronic kidney disease (CKD) and end-stage renal disease (ESRD) compared with general population; however, data on the differences in the occurrence of CKD and ESRD between patients with psoriasis and non-psoriatic controls are limited and inconsistent. The aim of this study was to carry out a comparison of the probability of suffering CKD and ESRD in patients with or without psoriasis by conducting a meta-analysis of cohort studies.MethodsCohort studies on PubMed, Web of Science, Embase and Cochrane Library by March, 2023 were searched for. The studies were screened according to pre-established inclusion criteria. Hazard ratios (HRs) and a 95% confidence intervals (CIs) for the renal outcomes among patients with psoriasis were calculated using the random-effect, generic inverse variance method. Subgroup analysis was related to the severity of psoriasis.ResultsA total of seven retrospective cohort studies were included, including 738,104 psoriasis patients and 3,443,438 non-psoriasis subjects, published from 2013 to 2020. Compared to controls without psoriasis, patients with psoriasis had an increased risk of CKD and ESRD, with pooled hazard ratios of 1.65 (95% CI, 1.29–2.12) and 1.37 (95% CI, 1.14–1.64), respectively. Besides, the incidence of CKD and ESRD is positively correlated with the severity of psoriasis.ConclusionThis study showed that compared to patients without psoriasis, patients with psoriasis, especially those with severe psoriasis, had a significantly increased risk of developing CKD and ESRD. Considering the limitations of this meta-analysis, more high-quality and well-designed studies are needed in the future to validate our findings.

In order to improve the translation quality of complex English sentences, this paper investigated unknown words. First, two baseline models, the recurrent neural machine translation (RNMT) model and the transformer model, were briefly introduced. Then, the unknown words were identified and replaced based on WordNet and the semantic environment and input to the neural machine translation (NMT) model for translation. Finally, experiments were conducted on several National Institute of Standards and Technology (NIST) datasets. It was found that the transformer model significantly outperformed the RNMT model, its average bilingual evaluation understudy (BLEU) value was 42.14, which was 6.96 higher than the RNMT model, and its translation error rate (TER) was also smaller. After combining the intelligent algorithm, the BLEU values of both models improved, and the TER became smaller; the average BLEU value of the transformer model combined with the intelligent algorithm was 43.7, and the average TER was 57.68. The experiment verifies that the transformer model combined with the intelligent algorithm is reliable in translating complex sentences and can obtain higher-quality translation results.

ObjectiveTo study the annual variation of diatoms in Zhangweixin River, to provide theoretical support by using diatom examination to estimate the time and place of the corpse entering water, and to establish a diatom database.MethodsSamples were taken from 4 sampling sites in Decheng section of Zhangweixin River for 12 consecutive months. Non-metric multi-dimensional scaling （NMDS） analysis was performed on the species and content of diatom samples. Based on the sampling site of Tianqu Road, Sprensen similarity coefficient analysis was conducted with the data of other 3 sites in Decheng section and Leling section of Zhangweixin River and Ningjin section in previous studies.ResultsThe number of diatom species was positively correlated with diatom content. The average diatom content in different months ranged from 1 054 to 13 041/10 mL, and the species composition ranged from 8 to 16, with differences in dominant species. The similarity coefficient of diatom species within 2 km were all higher than 0.956 52， which could not be distinguished effectively. The similarity coefficients of Leling section and Ningjin section were 0.736 84 and 0.588 24 respectively, which could be effectively distinguished.ConclusionThe species and content of diatom vary in different months in Zhangweixin River, and the composition of diatom species is different in different basins, which can provide reference for estimating the time and place of the corpse entering water in the river.

In view of the low accuracy of breast shape recognition, the approach of CFSFDP was used to cluster the breast shape of 108 young female breast forms in west region, and then the ELM algorithm was used to identify breast shape. The relationship between the number of neurons in the hidden layer and the accuracy of the ELM model under three activation functions was compared and analyzed. The results showed that the breast shape recognition by ELM model has high accuracy and short time, with an average time of 1.28 s. When the activation function of the model was sin and the number of neurons in the hidden layer was set to 25, the average accuracy of breast shape recognition was 98.3%. The research on parameter setting of ELM breast shape recognition model improves the accuracy of breast shape recognition and the compatibility between consumer breast and bra size to a certain extent. It also provides a basis for parameter selection of human body shape recognition research.

ObjectiveTo analyze the intake and source of fat and fatty acids of urban residents in Guangdong Province.MethodsResidents aged 3 years and above were selected by multi-stage stratified cluster sampling method in 5 cities in Guangdong Province from 2017 to 2018, and non-continuous 3 d-24 h retrospective survey was conducted to collect the dietary intake information of the respondents.ResultsThe average daily energy intake and fat intake of urban residents in Guangdong Province were 1671.9 kcal and 69.2 g, and the energy proportion contributed from fat was 39.4%. The energy proportion contributed from fat of urban residents in big cities was higher than that in small and medium-sized cities (39.9% vs 39.0%, P < 0.05). 1.6% of the residents’ energy proportion contributed from fat was lower than 20%, 14.8% of the residents' proportion met the standard, and 83.6% of the residents’ proportion was 20%-30%. Fat intake mainly came from animal food (52.8%) and vegetable oil (30.9%). The proportion of total fat from vegetable oil in small and medium-sized cities was higher than that in large cities (32.7% vs 27.7%, P

A large shaking-table test of a loess slope with a geometric similarity ratio of 1:25 was established as a prototype model of the loess landslide in Yongguang Village, which occurred during the 2013 Minxian–Zhangxian M6.6 earthquake. Combined with finite-element–numerical-simulation software, the dynamic response characteristics of the loess slope were compared and analyzed under the action of the same Minxian horizontal seismic wave characteristics. The results show that the seismic waves observed in the model test and numerical simulation clearly exhibit non-linear amplification effects along the slope surface and in the loess vertical direction, reaching a maximum value at the top of the slope. The ground-motion acceleration waveforms observed in the model test and numerical simulation at each observation point of the slope were similar, and the horizontal peak ground acceleration (PGA) obtained from the numerical simulation at these locations was larger than that of the slope top. The horizontal aSimulated-max/aModel-max values ranged from 1.5 to 2.2 and the vertical aSimulated-max/aModel-max values were more discrete in the range of 0.5–2.7, while the horizontal and vertical-PGA-amplification factors at the slope surface were similar. The Fourier amplitude and main horizontal frequencies were mainly distributed between 0.1 and 12 Hz. The Fourier spectrum observed in the model test was approximately 0.5 Hz, while the main frequency of the vertical Fourier spectrum was approximately 4 Hz. Additionally, the main frequency of the horizontal and vertical Fourier spectra observed in the numerical simulation was approximately 2 and 1.2 Hz, respectively.

Objective To explore the distribution of immune cell subsets in patients with chronic graft-versus-host disease (cGVHD) and investigate the predictive values of specific cell subsets sorted by surface markers in the development of cGVHD. Methods From October 2020 to July 2021, 58 patients who received hematopoietic stem cell transplantation (HSCT) from the Hematology Medical Center of the Second Affiliated Hospital of the Army Medical University were collected in this study. Flow cytometry was used to detect the ratio of immune cell subpopulations in the cGVHD patients (cGVHD group, n=43) and those without (control group, n=15) who received allogeneic HSCT during the same period. Primary diagnoses of the recruited patients included acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), chronic myeloid leukemia (CML), myelodysplastic syndromes (MDS), severe aplastic anemia (SAA), and β-thalassemia major. The median time to occurrence of cGVHD was 178.5 (47~1 328) d. Peripheral blood (PB) samples were collected for each patient when cGVHD occurred during follow-up or on the day of follow-up during the course of the disease. Results The percentages of PB regulatory T cells (Tregs) and transitional B cells were significantly reduced in the cGVHD group (P < 0.05), and that of CD19+ B lymphocytes was higher than the control group. The percentage of Tregs differed from the cGVHD patients with different severities (P < 0.05). The percentage of Th17-like cells in the patients with moderate cGVHD was significantly higher than patients with mild cGVHD. CD19+ B lymphocytes showed higher percentage in the patients with mild and moderate cGVHD. In the comparison among different targeted organs of cGVHD, only the Tregs percentage was significantly lower in the patients with skin and liver damage than control group, while the percentage of follicular helper T cells in the patients with skin and liver damage was significantly higher than those only having mucous membrane or eye organ involvement. Conclusion Distribution of immune cell subsets after HSCT is an important reference marker for the diagnosis, treatment and prognosis of cGVHD. Treg cells and transitional B cells which are sorted with a special combined strategy of surface markers play specific guiding roles in the pathogenesis, severity and damage of targeted organs of cGVHD.

Stable isotope ratios of carbon, nitrogen, hydrogen and oxygen act as “nature’s recorder”. As a carrier for individual human information, human hair can be measured by isotope ratio mass spectrometry (IRMS) to translate into information about people’s dietary habits, health condition and life track. The basic principle behind establishing dietary lifestyle using isotopic profiles of human hair is the fact that the body’s only source of C, N is a person’s staple diet. It may have significant differences in carbon, nitrogen isotopic compositions of human hair that are living at different regions. The aim of the study was to analyze the discrepancy of 15N and 13C stable isotope ratios in human hair collected from different cities, and explore the relationship between carbon and nitrogen isotope ratios and human groups, such as gender and age. The carbon and nitrogen isotope ratios in the human hair of 247 local residents from 8 different cities were investigated by IRMS. The results showed that there were significant difference in carbon and nitrogen stable isotope ratios of human hair from different cities. The value of δ13C was negatively correlated with vegetable consumption per capita. The value of δ15N was positively correlated with meat consumption per capita, and might be increased by high intake of fish. The relationship between δ13C, δ15N and the factors of gender and age demonstrated that there were no significant difference between males and females, but the age of females showed a weakly negative correlation with δ13C. By comparing the data of this paper and other literatures, the carbon and nitrogen stable isotopes in the hair of residents in China were different from other countries, which implied that there were various dietary habits in different countries.

Objective To investigate the laboratory and clinical characteristics of acute lymphoblastic leukemia (ALL) with e1a3 BCR-ABL fusion protein. Methods The clinical data, and results of bone marrow cytology, flow cytometry, karyotyping, fluorescence in situ hybridization (FISH) and real-time fluorescence quantitative PCR of 2 ALL cases with e1a3 BCR-ABL fusion protein admitted in our department were collected and analyzed. The related literature was reviewed. Results Both cases presented ALL phenotype. FISH test showed BCR-ABL positive, and RQ-PCR (p190, p210, p230) negative. Finally, the rare BCR-ABL fusion gene test was added and the 2 patients were diagnosed as rare e1a3 BCR-ABL positive ALL. One patient received TKI treatment and had achieved complete remission for more than 4 months. Another patient did not receive TKI and died in longer than 1 month after diagnosis. Conclusion Multi-technique joint detection for fusion gene is helpful for the detection and diagnosis of ALL with e1a3 BCR-ABL fusion protein, and TKI therapy is helpful to improve the prognosis.

This study aimed to investigate the prevalence of age-related macular degeneration (AMD) in patients with diabetes mellitus (DM) and diabetic retinopathy (DR) and analyze whether DR is a risk factor for AMD. This population-based epidemiological study included 14,440 people from the Kailuan Eye Study in 2016, of whom 1,618 were patients with type 2 DM aged over 50 years, and 409 had DM with DR. We analyzed whether there were differences in the prevalence of AMD between DM with DR and DM without DR, and conducted a hierarchical statistical analysis according to different stages of DR. Using variable regression analysis, we explored whether DR constituted a risk factor for AMD. In the DM population, the prevalence of wet AMD in patients with DM with and without DR was 0. 3 and 0.2%, respectively, with no significant difference (P = 0.607). Meanwhile, the prevalence of dry AMD in patients with DM with and without DR was 20.8 and 16.0%, respectively, with a significant difference. In the subgroup analysis of dry AMD, the prevalence of early, middle, and late dry AMD in DM with DR was 14.4, 5.9, and 0.5%, respectively. In DM without DR, the prevalence of early, middle, and late dry AMD was 10.5, 4.8, and 0.7%, respectively (P = 0.031). In the subgroup analysis of DR staging, statistical analysis could not be performed because of the limited number of patients with PDR. In the variable regression analysis of risk factors for dry AMD, after adjusting for age, sex, body mass index, hypertension, and dyslipidemia, DR constituted the risk factor for dry AMD. In conclusion, DM did not constitute a risk factor for AMD, and the prevalence of wet AMD and dry AMD in patients with DM and DR was higher than that in patients with DM without DR (among which dry AMD was statistically significant). Multivariate regression analysis confirmed that DR is an independent risk factor for dry AMD. Reasonable control of DM and slowing down the occurrence and development of DR may effectively reduce the prevalence of AMD in patients with DM.

With the continuous evolution of the mobile communication generation, human society has gradually convert from the era of “Mobile Internet” to the era of “Internet of Everything”. 5G networks will break the limitations of the traditional mobile communication industry and reshape the social development model. 5G will realize a new round of information revolution. As a new generation of mobile communication technology, 5G could provide basic communication guarantee for current innovative applications, such as the Internet of Things, autonomous vehicles and industrial Internet, and meet the explosive growth of data traffic and massive device connections. Security is a core for the comprehensive promotion of 5G networks. This paper analyzes the security risks in 5G networks and clarifies its security requirements. Finally, it proposes the corresponding security architecture, which will provide reference for the subsequent development of 5G network security technology.