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Your search keyword '"WAFL/FKBP15/FKBP133/KIAA0674"' showing total 2 results
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2 results on '"WAFL/FKBP15/FKBP133/KIAA0674"'

1. The ulcerative colitis marker protein WAFL interacts with accessory proteins in endocytosis

Author

Sven Pettersson, Ing-Marie Viklund, Ichiro N. Maruyama, Heng Hang Tsai, and You Fu Pan

Subjects
Proteomics, Cell type, Immunoprecipitation, Endocytosis, Applied Microbiology and Biotechnology, Inflammatory bowel disease, Models, Biological, Mass Spectrometry, Cell Line, WAFL/FKBP15/FKBP133/KIAA0674, Tacrolimus Binding Proteins, Cell Line, Tumor, medicine, Humans, Molecular Biology, Gene, Ecology, Evolution, Behavior and Systematics, KIAA1033, Wiskott-Aldrich syndrome protein, biology, Macrophages, Wiskott–Aldrich syndrome protein, Proteins, KIAA0196/strumpellin, Cell Biology, medicine.disease, Molecular biology, Cell biology, Wiskott-Aldrich Syndrome Protein Family, Transcription Factor AP-2, Cell culture, biology.protein, Colitis, Ulcerative, Colorectal Neoplasms, Developmental Biology, Chromatography, Liquid, Research Paper
Abstract

Ulcerative colitis (UC) is one of the major forms of inflammatory bowel disease with unknown cause. A molecular marker, WAFL, has recently been found to be up-regulated in the inflamed colonic mucosa of UC patients. Towards understanding biological function of WAFL, we analyzed proteins interacting with WAFL in HEK-293 cells by immunoprecipitation and mass spectrometry. Among four proteins found to specifically interact with WAFL, both KIAA0196 and KIAA1033 bind to alpha-appendage of the adaptor protein complex 2 (AP2), which acts as an interaction hub for accessory proteins in endocytosis mediated by clathrin-coated vesicle (CCV). The specific interaction between WAFL and KIAA0196 was also confirmed in human colorectal carcinoma HCT-116 cells by co-immunoprecipitation with specific antibodies. Meta-analyses of the databases of expressed genes suggest that the three genes are co-expressed in many tissues and cell types, and that their molecular function may be classified in the category of 'membrane traffic protein'. Therefore, these results suggest that WAFL may play an important role in endocytosis and subsequent membrane trafficking by interacting with AP2 through KIAA0196 and KIAA1033.

Published
2009

2. The ulcerative colitis marker protein WAFL interacts with accessory proteins in endocytosis.

Author

Pan YF, Viklund IM, Tsai HH, Pettersson S, and Maruyama IN

Subjects
Cell Line, Cell Line, Tumor, Chromatography, Liquid methods, Colorectal Neoplasms pathology, Humans, Macrophages metabolism, Mass Spectrometry methods, Models, Biological, Proteins metabolism, Proteomics methods, Wiskott-Aldrich Syndrome Protein metabolism, Colitis, Ulcerative pathology, Endocytosis, Tacrolimus Binding Proteins metabolism, Transcription Factor AP-2 metabolism, Wiskott-Aldrich Syndrome Protein Family metabolism
Abstract

Ulcerative colitis (UC) is one of the major forms of inflammatory bowel disease with unknown cause. A molecular marker, WAFL, has recently been found to be up-regulated in the inflamed colonic mucosa of UC patients. Towards understanding biological function of WAFL, we analyzed proteins interacting with WAFL in HEK-293 cells by immunoprecipitation and mass spectrometry. Among four proteins found to specifically interact with WAFL, both KIAA0196 and KIAA1033 bind to alpha-appendage of the adaptor protein complex 2 (AP2), which acts as an interaction hub for accessory proteins in endocytosis mediated by clathrin-coated vesicle (CCV). The specific interaction between WAFL and KIAA0196 was also confirmed in human colorectal carcinoma HCT-116 cells by co-immunoprecipitation with specific antibodies. Meta-analyses of the databases of expressed genes suggest that the three genes are co-expressed in many tissues and cell types, and that their molecular function may be classified in the category of 'membrane traffic protein'. Therefore, these results suggest that WAFL may play an important role in endocytosis and subsequent membrane trafficking by interacting with AP2 through KIAA0196 and KIAA1033.

Published
2010
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