1. Nuclear DNA content of the hybrid plant pathogenPhytophthora andinadetermined by flow cytometry
- Author
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Jackson W. Presser, Erica M. Goss, and Jianan Wang
- Subjects
Phytophthora ,0106 biological sciences ,0301 basic medicine ,Physiology ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Botany ,Genetics ,Heterothallic ,Mexico ,Molecular Biology ,Pathogen ,Genome size ,Ecology, Evolution, Behavior and Systematics ,biology ,DNA ,Cell Biology ,General Medicine ,Plants ,Flow Cytometry ,biology.organism_classification ,Nuclear DNA ,030104 developmental biology ,chemistry ,Phytophthora infestans ,Ploidy ,010606 plant biology & botany - Abstract
Phytophthora andina is a heterothallic plant pathogen of Andean solanaceous hosts and is an interspecific hybrid of P. infestans and an unknown Phytophthora species. The objective of this study was to estimate the nuclear DNA content of isolates in three clonal lineages of P. andina relative to P. infestans Twelve isolates of P. andina and six isolates of P. infestans were measured for nuclear DNA content by propidium iodide-stained flow cytometry. We found that the DNA content of P. andina was similar but slightly smaller, on average, than that of our sample of P. infestans isolates. This is consistent with P. andina being a homoploid hybrid rather than allopolyploid hybrid. Nuclear DNA content was more variable among a smaller sample of P. infestans isolates, including a putative triploid isolate from Mexico, but small differences in nuclear DNA content were also observed among P. andina isolates. Both species appear to be able to tolerate significant variation in genome size.
- Published
- 2016
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