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13 results on '"Vries, Sippe"'

1. Nuclear chromosome locations dictate segregation error frequencies

Author

Klaasen, Sjoerd J., Truong, My Anh, van Jaarsveld, Richard H., Koprivec, Isabella, Štimac, Valentina, de Vries, Sippe G., Risteski, Patrik, Kodba, Snježana, Vukušić, Kruno, de Luca, Kim L., Marques, Joana F., Gerrits, Elianne M., Bakker, Bjorn, Foijer, Floris, Kind, Jop, Tolić, Iva M., Lens, Susanne M. A., and Kops, Geert J. P. L.

Published
2022
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3. Reconstructing single-cell karyotype alterations in colorectal cancer identifies punctuated and gradual diversification patterns

Author

Bollen, Yannik, Stelloo, Ellen, van Leenen, Petra, van den Bos, Myrna, Ponsioen, Bas, Lu, Bingxin, van Roosmalen, Markus J., Bolhaqueiro, Ana C. F., Kimberley, Christopher, Mossner, Maximilian, Cross, William C. H., Besselink, Nicolle J. M., van der Roest, Bastiaan, Boymans, Sander, Oost, Koen C., de Vries, Sippe G., Rehmann, Holger, Cuppen, Edwin, Lens, Susanne M. A., Kops, Geert J. P. L., Kloosterman, Wigard P., Terstappen, Leon W. M. M., Barnes, Chris P., Sottoriva, Andrea, Graham, Trevor A., and Snippert, Hugo J. G.

Published
2021
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5. A kinesin-based approach for inducing chromosome-specific mis-segregation in human cells

Author

Sub Cell Biology, Celbiologie, Truong, My Anh, Cané-Gasull, Paula, de Vries, Sippe G, Nijenhuis, Wilco, Wardenaar, René, Kapitein, Lukas C, Foijer, Floris, Lens, Susanne, Sub Cell Biology, Celbiologie, Truong, My Anh, Cané-Gasull, Paula, de Vries, Sippe G, Nijenhuis, Wilco, Wardenaar, René, Kapitein, Lukas C, Foijer, Floris, and Lens, Susanne

Published
2023

6. A kinesin-based approach for inducing chromosome-specific mis-segregation in human cells

Author

CMM, CMM Groep Lens, Cancer, Truong, My Anh, Cané-Gasull, Paula, de Vries, Sippe G., Nijenhuis, Wilco, Wardenaar, René, Kapitein, Lukas C., Foijer, Floris, Lens, Susanne M.A., CMM, CMM Groep Lens, Cancer, Truong, My Anh, Cané-Gasull, Paula, de Vries, Sippe G., Nijenhuis, Wilco, Wardenaar, René, Kapitein, Lukas C., Foijer, Floris, and Lens, Susanne M.A.

Published
2023

7. Nuclear chromosome locations dictate segregation error frequencies

Author

Klaasen, Sjoerd J, Truong, My Anh, van Jaarsveld, Richard H, Koprivec, Isabella, Štimac, Valentina, de Vries, Sippe G, Risteski, Patrik, Kodba, Snježana, Vukušić, Kruno, de Luca, Kim L, Marques, Joana F, Gerrits, Elianne M, Bakker, Bjorn, Foijer, Floris, Kind, Jop, Tolić, Iva M, Lens, Susanne M A, Kops, Geert J P L, Klaasen, Sjoerd J, Truong, My Anh, van Jaarsveld, Richard H, Koprivec, Isabella, Štimac, Valentina, de Vries, Sippe G, Risteski, Patrik, Kodba, Snježana, Vukušić, Kruno, de Luca, Kim L, Marques, Joana F, Gerrits, Elianne M, Bakker, Bjorn, Foijer, Floris, Kind, Jop, Tolić, Iva M, Lens, Susanne M A, and Kops, Geert J P L

Abstract

Chromosome segregation errors during cell divisions generate aneuploidies and micronuclei, which can undergo extensive chromosomal rearrangements such as chromothripsis1-5. Selective pressures then shape distinct aneuploidy and rearrangement patterns-for example, in cancer6,7-but it is unknown whether initial biases in segregation errors and micronucleation exist for particular chromosomes. Using single-cell DNA sequencing8 after an error-prone mitosis in untransformed, diploid cell lines and organoids, we show that chromosomes have different segregation error frequencies that result in non-random aneuploidy landscapes. Isolation and sequencing of single micronuclei from these cells showed that mis-segregating chromosomes frequently also preferentially become entrapped in micronuclei. A similar bias was found in naturally occurring micronuclei of two cancer cell lines. We find that segregation error frequencies of individual chromosomes correlate with their location in the interphase nucleus, and show that this is highest for peripheral chromosomes behind spindle poles. Randomization of chromosome positions, Cas9-mediated live tracking and forced repositioning of individual chromosomes showed that a greater distance from the nuclear centre directly increases the propensity to mis-segregate. Accordingly, chromothripsis in cancer genomes9 and aneuploidies in early development10 occur more frequently for larger chromosomes, which are preferentially located near the nuclear periphery. Our findings reveal a direct link between nuclear chromosome positions, segregation error frequencies and micronucleus content, with implications for our understanding of tumour genome evolution and the origins of specific aneuploidies during development.

Published
2022

8. Nuclear chromosome locations dictate segregation error frequencies

Author

CMM Groep Lens, Cancer, Genetica Sectie Genoomdiagnostiek, BMW - Groep van Mil, CMM, Hubrecht Institute with UMC, Klaasen, Sjoerd J, Truong, My Anh, van Jaarsveld, Richard H, Koprivec, Isabella, Štimac, Valentina, de Vries, Sippe G, Risteski, Patrik, Kodba, Snježana, Vukušić, Kruno, de Luca, Kim L, Marques, Joana F, Gerrits, Elianne M, Bakker, Bjorn, Foijer, Floris, Kind, Jop, Tolić, Iva M, Lens, Susanne M A, Kops, Geert J P L, CMM Groep Lens, Cancer, Genetica Sectie Genoomdiagnostiek, BMW - Groep van Mil, CMM, Hubrecht Institute with UMC, Klaasen, Sjoerd J, Truong, My Anh, van Jaarsveld, Richard H, Koprivec, Isabella, Štimac, Valentina, de Vries, Sippe G, Risteski, Patrik, Kodba, Snježana, Vukušić, Kruno, de Luca, Kim L, Marques, Joana F, Gerrits, Elianne M, Bakker, Bjorn, Foijer, Floris, Kind, Jop, Tolić, Iva M, Lens, Susanne M A, and Kops, Geert J P L

Published
2022

10. Reconstructing single-cell karyotype alterations in colorectal cancer identifies punctuated and gradual diversification patterns

Author

CMM Sectie Molecular Cancer Research, CMM Groep Snippert, Regenerative Medicine and Stem Cells, Cancer, Genetica, MS Medische Oncologie, CMM Groep Kloosterman, Lab Reumatologie/Klinische Immunologie, Epi Infectieziekten Team 2, Infection & Immunity, CMM USEQ Facility, CMM Groep Bos, CMM Groep Lens, CMM, CMM Groep Cuppen, Hubrecht Institute with UMC, CMM Groep De Ridder, Child Health, Bollen, Yannik, Stelloo, Ellen, van Leenen, Petra, van den Bos, Myrna, Ponsioen, Bas, Lu, Bingxin, van Roosmalen, Markus J., Bolhaqueiro, Ana C.F., Kimberley, Christopher, Mossner, Maximilian, Cross, William C.H., Besselink, Nicolle J.M., van der Roest, Bastiaan, Boymans, Sander, Oost, Koen C., de Vries, Sippe G., Rehmann, Holger, Cuppen, Edwin, Lens, Susanne M.A., Kops, Geert J.P.L., Kloosterman, Wigard P., Terstappen, Leon W.M.M., Barnes, Chris P., Sottoriva, Andrea, Graham, Trevor A., Snippert, Hugo J.G., CMM Sectie Molecular Cancer Research, CMM Groep Snippert, Regenerative Medicine and Stem Cells, Cancer, Genetica, MS Medische Oncologie, CMM Groep Kloosterman, Lab Reumatologie/Klinische Immunologie, Epi Infectieziekten Team 2, Infection & Immunity, CMM USEQ Facility, CMM Groep Bos, CMM Groep Lens, CMM, CMM Groep Cuppen, Hubrecht Institute with UMC, CMM Groep De Ridder, Child Health, Bollen, Yannik, Stelloo, Ellen, van Leenen, Petra, van den Bos, Myrna, Ponsioen, Bas, Lu, Bingxin, van Roosmalen, Markus J., Bolhaqueiro, Ana C.F., Kimberley, Christopher, Mossner, Maximilian, Cross, William C.H., Besselink, Nicolle J.M., van der Roest, Bastiaan, Boymans, Sander, Oost, Koen C., de Vries, Sippe G., Rehmann, Holger, Cuppen, Edwin, Lens, Susanne M.A., Kops, Geert J.P.L., Kloosterman, Wigard P., Terstappen, Leon W.M.M., Barnes, Chris P., Sottoriva, Andrea, Graham, Trevor A., and Snippert, Hugo J.G.

Published
2021

11. Human chromosome-specific aneuploidy is influenced by DNA-dependent centromeric features

Author

CMM Groep Lens, Cancer, Hubrecht Institute with UMC, Dumont, Marie, Gamba, Riccardo, Gestraud, Pierre, Klaasen, Sjoerd, Worrall, Joseph T., De Vries, Sippe G., Boudreau, Vincent, Salinas-Luypaert, Catalina, Maddox, Paul S., Lens, Susanne M.A., Kops, Geert J.P.L., McClelland, Sarah E., Miga, Karen H., Fachinetti, Daniele, CMM Groep Lens, Cancer, Hubrecht Institute with UMC, Dumont, Marie, Gamba, Riccardo, Gestraud, Pierre, Klaasen, Sjoerd, Worrall, Joseph T., De Vries, Sippe G., Boudreau, Vincent, Salinas-Luypaert, Catalina, Maddox, Paul S., Lens, Susanne M.A., Kops, Geert J.P.L., McClelland, Sarah E., Miga, Karen H., and Fachinetti, Daniele

Published
2020

12. Human chromosome‐specific aneuploidy is influenced byDNA‐dependent centromeric features

Author

Dumont, Marie, primary, Gamba, Riccardo, additional, Gestraud, Pierre, additional, Klaasen, Sjoerd, additional, Worrall, Joseph T, additional, De Vries, Sippe G, additional, Boudreau, Vincent, additional, Salinas‐Luypaert, Catalina, additional, Maddox, Paul S, additional, Lens, Susanne MA, additional, Kops, Geert JPL, additional, McClelland, Sarah E, additional, Miga, Karen H, additional, and Fachinetti, Daniele, additional

Published
2019
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13. Human chromosome-specific aneuploidy is influenced by DNA-dependent centromeric features

Author

Dumont, Marie, Gamba, Riccardo, Gestraud, Pierre, Klaasen, Sjoerd, Worrall, Joseph T, De Vries, Sippe G, Boudreau, Vincent, Salinas-Luypaert, Catalina, Maddox, Paul S, Lens, Susanne Ma, Kops, Geert Jpl, McClelland, Sarah E, Miga, Karen H, Fachinetti, Daniele, Dumont, Marie, Gamba, Riccardo, Gestraud, Pierre, Klaasen, Sjoerd, Worrall, Joseph T, De Vries, Sippe G, Boudreau, Vincent, Salinas-Luypaert, Catalina, Maddox, Paul S, Lens, Susanne Ma, Kops, Geert Jpl, McClelland, Sarah E, Miga, Karen H, and Fachinetti, Daniele

Abstract

Intrinsic genomic features of individual chromosomes can contribute to chromosome-specific aneuploidy. Centromeres are key elements for the maintenance of chromosome segregation fidelity via a specialized chromatin marked by CENP-A wrapped by repetitive DNA. These long stretches of repetitive DNA vary in length among human chromosomes. Using CENP-A genetic inactivation in human cells, we directly interrogate if differences in the centromere length reflect the heterogeneity of centromeric DNA-dependent features and whether this, in turn, affects the genesis of chromosome-specific aneuploidy. Using three distinct approaches, we show that mis-segregation rates vary among different chromosomes under conditions that compromise centromere function. Whole-genome sequencing and centromere mapping combined with cytogenetic analysis, small molecule inhibitors, and genetic manipulation revealed that inter-chromosomal heterogeneity of centromeric features, but not centromere length, influences chromosome segregation fidelity. We conclude that faithful chromosome segregation for most of human chromosomes is biased in favor of centromeres with high abundance of DNA-dependent centromeric components. These inter-chromosomal differences in centromere features can translate into non-random aneuploidy, a hallmark of cancer and genetic diseases.

Published
2019

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