Search

Your search keyword '"Vrieling, Manouk"' showing total 38 results
Start Over Author "Vrieling, Manouk"
38 results on '"Vrieling, Manouk"'

1. Porcine Airway Organoid-Derived Well-Differentiated Epithelial Cultures as a Tool for the Characterization of Swine Influenza a Virus Strains.

Author

Gerhards, Nora M., Vrieling, Manouk, Dresken, Romy, Nguyen-van Oort, Sophie, Bordes, Luca, Wells, Jerry M., and de Swart, Rik L.

Abstract

Swine influenza A viruses (IAVsw) are important causes of disease in pigs but also constitute a public health risk. IAVsw strains show remarkable differences in pathogenicity. We aimed to generate airway organoids from the porcine lower respiratory tract and use these to establish well-differentiated airway epithelial cell (WD-AEC) cultures grown at an air–liquid interface (ALI) for in vitro screening of IAVsw strain virulence. Epithelial cells were isolated from bronchus tissue of juvenile pigs, and airway organoids were cultured in an extracellular matrix in a culture medium containing human growth factors. Single-cell suspensions of these 3D organoids were seeded on Transwell filters and differentiated at ALI to form a pseudostratified epithelium containing ciliated cells, mucus-producing cells and tight junctions. Inoculation with a low dose of IAVsw in a low volume inoculum resulted in virus replication without requiring the addition of trypsin, and was quantified by the detection of viral genome loads in apical washes. Interestingly, inoculation of an H3N2 strain known to cause severe disease in pigs induced a greater reduction in trans-epithelial resistance and more damage to tight junctions than H1N2 or H1N1 strains associated with mild disease in pigs. We conclude that the porcine WD-AEC model is useful in assessing the virulence of IAVsw strains. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

3. Experimental evolution of Staphylococcus aureus in macrophages: dissection of a conditional adaptive trait promoting intracellular survival

Author

Alves, Joana, Vrieling, Manouk, Ring, Natalie, Yebra, Gonzalo, Pickering, Amy, Prajsnar, Tomasz, Renshaw, Stephen, Fitzgerald, J., Alves, Joana, Vrieling, Manouk, Ring, Natalie, Yebra, Gonzalo, Pickering, Amy, Prajsnar, Tomasz, Renshaw, Stephen, and Fitzgerald, J.

Abstract

Staphylococcus aureus is a major pathogen associated with important diseases in humans and animals. Macrophages are a key component of the innate immune response to S. aureus infection and play a major role in disease outcomes. To investigate the adaptive evolution of S. aureus in response to macrophages, we developed an experimental infection assay. S. aureus strains representing major human epidemic clones were passaged many times in a macrophage cell line, accumulating mutations in an array of genomic loci. Phenotypic analysis revealed the emergence of a lineage exhibiting increased survival in macrophages and human blood, and resistance to vancomycin. The evolved lineage exhibited a previously undescribed small colony variant (SCV) phenotype characterized by hyper-pigmentation, which resulted from a missense mutation in rsbW. Notably, the novel SCV was a conditional adaptive trait that was unstable in nutrient-replete conditions in vitro, rapidly converting from hyper-pigmented SCV to a non-pigmented large colony variant via spontaneous sigB deletion events. Importantly, we identified similar deletions in the genome sequences of a limited number of clinical S. aureus isolates from public databases, indicating that related events may occur during clinical infection. Experimental infection of zebrafish did not reveal a difference in virulence between parent and novel SCV but demonstrated an in vivo fitness cost for the compensatory sigB deletion events. Taken together, we report an experimental evolutionary approach for investigating bacterial innate immune cell interactions, revealing a conditional adaptation that promotes S. aureus survival in macrophages and resistance to vancomycin.

Published
2024

4. The emergence and diversification of a zoonotic pathogen from within the microbiota of intensively farmed pigs

Author

Murray, Gemma G. R., primary, Hossain, A. S. Md. Mukarram, additional, Miller, Eric L., additional, Bruchmann, Sebastian, additional, Balmer, Andrew J., additional, Matuszewska, Marta, additional, Herbert, Josephine, additional, Hadjirin, Nazreen F., additional, Mugabi, Robert, additional, Li, Ganwu, additional, Ferrando, Maria Laura, additional, Fernandes de Oliveira, Isabela Maria, additional, Nguyen, Thanh, additional, Yen, Phung L. K., additional, Phuc, Ho D., additional, Zaw Moe, Aung, additional, Su Wai, Thiri, additional, Gottschalk, Marcelo, additional, Aragon, Virginia, additional, Valentin-Weigand, Peter, additional, Heegaard, Peter M. H., additional, Vrieling, Manouk, additional, Thein Maw, Min, additional, Thidar Myint, Hnin, additional, Tun Win, Ye, additional, Thi Hoa, Ngo, additional, Bentley, Stephen D., additional, Clavijo, Maria J., additional, Wells, Jerry M., additional, Tucker, Alexander W., additional, and Weinert, Lucy A., additional

Published
2023
Full Text
View/download PDF

5. Staphylococcal Immune Evasion Proteins: Structure, Function, and Host Adaptation

Author

Koymans, Kirsten J., Vrieling, Manouk, Gorham, Ronald D., Jr., van Strijp, Jos A. G., Compans, Richard W, Series editor, Malissen, Bernard, Series editor, Aktories, Klaus, Series editor, Rappuoli, Rino, Series editor, Galan, Jorge E., Series editor, Ahmed, Rafi, Series editor, Palme, Klaus, Series editor, Casadevall, Arturo, Series editor, Garcia-Sastre, Adolfo, Series editor, Iwasaki, Akiko, Series editor, Bagnoli, Fabio, editor, and Grandi, Guido, editor

Published
2017
Full Text
View/download PDF

6. Gene exchange drives the ecological success of a multi-host bacterial pathogen

Author

Richardson, Emily J., Bacigalupe, Rodrigo, Harrison, Ewan M., Weinert, Lucy A., Lycett, Samantha, Vrieling, Manouk, Robb, Kirsty, Hoskisson, Paul A., Holden, Matthew T. G., Feil, Edward J., Paterson, Gavin K., Tong, Steven Y. C., Shittu, Adebayo, van Wamel, Willem, Aanensen, David M., Parkhill, Julian, Peacock, Sharon J., Corander, Jukka, Holmes, Mark, and Fitzgerald, J. Ross

Published
2018
Full Text
View/download PDF

7. The emergence and diversification of a zoonotic pathogen from within the microbiota of intensively farmed pigs

Author

Murray, Gemma G.R., Hossain, A. S.Md Mukarram, Miller, Eric L., Bruchmann, Sebastian, Balmer, Andrew J., Matuszewska, Marta, Herbert, Josephine, Hadjirin, Nazreen F., Mugabi, Robert, Li, Ganwu, Ferrando, Maria Laura, de Oliveira, Isabela Maria Fernandes, Nguyen, Thanh, Yen, Phung L.K., Phuc, Ho D., Moe, Aung Zaw, Wai, Thiri Su, Gottschalk, Marcelo, Aragon, Virginia, Valentin-Weigand, Peter, Heegaard, Peter M.H., Vrieling, Manouk, Maw, Min Thein, Myint, Hnin Thidar, Win, Ye Tun, Hoa, Ngo Thi, Bentley, Stephen D., Clavijo, Maria J., Wells, Jerry M., Tucker, Alexander W., Weinert, Lucy A., Murray, Gemma G.R., Hossain, A. S.Md Mukarram, Miller, Eric L., Bruchmann, Sebastian, Balmer, Andrew J., Matuszewska, Marta, Herbert, Josephine, Hadjirin, Nazreen F., Mugabi, Robert, Li, Ganwu, Ferrando, Maria Laura, de Oliveira, Isabela Maria Fernandes, Nguyen, Thanh, Yen, Phung L.K., Phuc, Ho D., Moe, Aung Zaw, Wai, Thiri Su, Gottschalk, Marcelo, Aragon, Virginia, Valentin-Weigand, Peter, Heegaard, Peter M.H., Vrieling, Manouk, Maw, Min Thein, Myint, Hnin Thidar, Win, Ye Tun, Hoa, Ngo Thi, Bentley, Stephen D., Clavijo, Maria J., Wells, Jerry M., Tucker, Alexander W., and Weinert, Lucy A.

Abstract

The expansion and intensification of livestock production is predicted to promote the emergence of pathogens. As pathogens sometimes jump between species, this can affect the health of humans as well as livestock. Here, we investigate how livestock microbiota can act as a source of these emerging pathogens through analysis of Streptococcus suis, a ubiquitous component of the respiratory microbiota of pigs that is also a major cause of disease on pig farms and an important zoonotic pathogen. Combining molecular dating, phylogeography, and comparative genomic analyses of a large collection of isolates, we find that several pathogenic lineages of S. suis emerged in the 19th and 20th centuries, during an early period of growth in pig farming. These lineages have since spread between countries and continents, mirroring trade in live pigs. They are distinguished by the presence of three genomic islands with putative roles in metabolism and cell adhesion, and an ongoing reduction in genome size, which may reflect their recent shift to a more pathogenic ecology. Reconstructions of the evolutionary histories of these islands reveal constraints on pathogen emergence that could inform control strategies, with pathogenic lineages consistently emerging from one subpopulation of S. suis and acquiring genes through horizontal transfer from other pathogenic lineages. These results shed light on the capacity of the microbiota to rapidly evolve to exploit changes in their host population and suggest that the impact of changes in farming on the pathogenicity and zoonotic potential of S. suis is yet to be fully realized.

Published
2023

8. The emergence and diversification of a zoonotic pathogen from within the microbiota of intensively farmed pigs

Author

Murray, Gemma, Hossain, Mukarram, Miller, Eric, Bruchmann, Sebastian, Balmer, Andrew, Matuszewska, Marta, Herbert, Josephine, Hadjirin, Nazreen, Mugabi, Robert, Li, Ganwu, Ferrando, Laura, Fernandes de Oliveira, Isabela M., Nguyen, Thanh, Yen, Phung, Phuc, Ho, Zaw moe, Aung, Su Wai, Thiri, Gottschalk, Marcelo, Aragon, Virginia, Valentin-Weigand, Peter, Heegaard, Peter, Vrieling, Manouk, Thein Maw, Min, Thidar myint, Hnin, Tun Win, Ye, Thi Hoa, Ngo, Bentley, Stephen, Clavijo, Maria, Wells, Jerry, Tucker, Alexander, Weinert, Lucy, Murray, Gemma, Hossain, Mukarram, Miller, Eric, Bruchmann, Sebastian, Balmer, Andrew, Matuszewska, Marta, Herbert, Josephine, Hadjirin, Nazreen, Mugabi, Robert, Li, Ganwu, Ferrando, Laura, Fernandes de Oliveira, Isabela M., Nguyen, Thanh, Yen, Phung, Phuc, Ho, Zaw moe, Aung, Su Wai, Thiri, Gottschalk, Marcelo, Aragon, Virginia, Valentin-Weigand, Peter, Heegaard, Peter, Vrieling, Manouk, Thein Maw, Min, Thidar myint, Hnin, Tun Win, Ye, Thi Hoa, Ngo, Bentley, Stephen, Clavijo, Maria, Wells, Jerry, Tucker, Alexander, and Weinert, Lucy

Abstract

There is growing concern that rapid growth in livestock production and major changes in farming practices are driving the emergence of pathogens capable of causing disease in both livestock and humans. However, most studies neglect livestock microbiota as a potential source of emerging pathogens. Here, we show how the global transport of live animals has facilitated the emergence of an important livestock and human zoonotic pathogen from a common member of the pig respiratory microbiota. Our results indicate that pathogenic lineages are likely to continue to emerge and diversify and recommend ways of controlling this.

Published
2023

10. Population Analysis of Staphylococcus aureus Reveals a Cryptic, Highly Prevalent Superantigen SElW That Contributes to the Pathogenesis of Bacteremia

Author

FAH veterinaire epidemiologie, Vrieling, Manouk, Tuffs, Stephen W, Yebra, Gonzalo, van Smoorenburg, Marleen Y, Alves, Joana, Pickering, Amy C, Park, Joo Youn, Park, Nogi, Heinrichs, David E, Benedictus, Lindert, Connelley, Timothy, Seo, Keun Seok, McCormick, John K, Fitzgerald, J Ross, FAH veterinaire epidemiologie, Vrieling, Manouk, Tuffs, Stephen W, Yebra, Gonzalo, van Smoorenburg, Marleen Y, Alves, Joana, Pickering, Amy C, Park, Joo Youn, Park, Nogi, Heinrichs, David E, Benedictus, Lindert, Connelley, Timothy, Seo, Keun Seok, McCormick, John K, and Fitzgerald, J Ross

Published
2020

12. Population Analysis of Staphylococcus aureus Reveals a Cryptic, Highly Prevalent Superantigen SElW That Contributes to the Pathogenesis of Bacteremia

Author

Vrieling, Manouk, primary, Tuffs, Stephen W., additional, Yebra, Gonzalo, additional, van Smoorenburg, Marleen Y., additional, Alves, Joana, additional, Pickering, Amy C., additional, Park, Joo Youn, additional, Park, Nogi, additional, Heinrichs, David E., additional, Benedictus, Lindert, additional, Connelley, Timothy, additional, Seo, Keun Seok, additional, McCormick, John K., additional, and Fitzgerald, J. Ross, additional

Published
2020
Full Text
View/download PDF

13. Identification of a staphylococcal complement inhibitor with broad host specificity in equid Staphylococcus aureus strains

Author

de Jong, Nienke W.M., Vrieling, Manouk, Garcia, Brandon L, Koop, Gerrit, Brettmann, Matt, Aerts, Piet C, Ruyken, Maartje, van Strijp, Jos A G, Holmes, Mark A., Harrison, Ewan M., Geisbrecht, Brian V, Rooijakkers, Suzan H. M., de Jong, Nienke W.M., Vrieling, Manouk, Garcia, Brandon L, Koop, Gerrit, Brettmann, Matt, Aerts, Piet C, Ruyken, Maartje, van Strijp, Jos A G, Holmes, Mark A., Harrison, Ewan M., Geisbrecht, Brian V, and Rooijakkers, Suzan H. M.

Published
2018

14. Identification of a staphylococcal complement inhibitor with broad host specificity in equid Staphylococcus aureus strains

Author

MMB Serologie, Infection & Immunity, De Jong, Nienke W.M., Vrieling, Manouk, Garcia, Brandon L., Koop, Gerrit, Brettmann, Matt, Aerts, Piet C., Ruyken, Maartje, Van Strijp, Jos A.G., Holmes, Mark, Harrison, Ewan M., Geisbrecht, Brian V., Rooijakkers, Suzan H.M., MMB Serologie, Infection & Immunity, De Jong, Nienke W.M., Vrieling, Manouk, Garcia, Brandon L., Koop, Gerrit, Brettmann, Matt, Aerts, Piet C., Ruyken, Maartje, Van Strijp, Jos A.G., Holmes, Mark, Harrison, Ewan M., Geisbrecht, Brian V., and Rooijakkers, Suzan H.M.

Published
2018

15. Identification of a staphylococcal complement inhibitor with broad host specificity in equid Staphylococcus aureus strains

Author

de Jong, Nienke W.M., primary, Vrieling, Manouk, additional, Garcia, Brandon L., additional, Koop, Gerrit, additional, Brettmann, Matt, additional, Aerts, Piet C., additional, Ruyken, Maartje, additional, van Strijp, Jos A.G., additional, Holmes, Mark, additional, Harrison, Ewan M., additional, Geisbrecht, Brian V., additional, and Rooijakkers, Suzan H.M., additional

Published
2018
Full Text
View/download PDF

16. The TLR2 Antagonist Staphylococcal Superantigen-Like Protein 3 Acts as a Virulence Factor to Promote Bacterial Pathogenicity in vivo

Author

Koymans, Kirsten J., Goldmann, Oliver, Karlsson, Christofer A.Q., Sital, Wiedjai, Thänert, Robert, Bisschop, Adinda, Vrieling, Manouk, Malmström, Johan, van Kessel, Kok P M, De Haas, Carla J.C., Van Strijp, Jos A.G., Medina, Eva, Koymans, Kirsten J., Goldmann, Oliver, Karlsson, Christofer A.Q., Sital, Wiedjai, Thänert, Robert, Bisschop, Adinda, Vrieling, Manouk, Malmström, Johan, van Kessel, Kok P M, De Haas, Carla J.C., Van Strijp, Jos A.G., and Medina, Eva

Published
2017

17. Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

Author

Koop, Gerrit, Vrieling, Manouk, Storisteanu, Daniel M L, Lok, Laurence S C, Monie, Tom, Van Wigcheren, Glenn, Raisen, Claire, Ba, Xiaoliang, Gleadall, Nicholas, Hadjirin, Nazreen, Timmerman, Arjen J., Wagenaar, Jaap A., Klunder, Heleen M., Fitzgerald, J. Ross, Zadoks, Ruth, Paterson, Gavin K., Torres, Carmen, Waller, Andrew S., Loeffler, Anette, Loncaric, Igor, Hoet, Armando E., Bergström, Karin, De Martino, Luisa, Pomba, Constança, De Lencastre, Hermínia, Ben Slama, Karim, Gharsa, Haythem, Richardson, Emily J., Chilvers, Edwin R., Gosselaar-de Haas, CJC, van Kessel, CPM, Van Strijp, Jos A G, Harrison, Ewan M., Holmes, Mark A., Koop, Gerrit, Vrieling, Manouk, Storisteanu, Daniel M L, Lok, Laurence S C, Monie, Tom, Van Wigcheren, Glenn, Raisen, Claire, Ba, Xiaoliang, Gleadall, Nicholas, Hadjirin, Nazreen, Timmerman, Arjen J., Wagenaar, Jaap A., Klunder, Heleen M., Fitzgerald, J. Ross, Zadoks, Ruth, Paterson, Gavin K., Torres, Carmen, Waller, Andrew S., Loeffler, Anette, Loncaric, Igor, Hoet, Armando E., Bergström, Karin, De Martino, Luisa, Pomba, Constança, De Lencastre, Hermínia, Ben Slama, Karim, Gharsa, Haythem, Richardson, Emily J., Chilvers, Edwin R., Gosselaar-de Haas, CJC, van Kessel, CPM, Van Strijp, Jos A G, Harrison, Ewan M., and Holmes, Mark A.

Published
2017

18. Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

Author

dFAH I&I, dFAH AVR, dI&I I&I-4, Koop, Gerrit, Vrieling, Manouk, Storisteanu, Daniel M L, Lok, Laurence S C, Monie, Tom, van Wigcheren, Glenn, Raisen, Claire, Ba, Xiaoliang, Gleadall, Nicholas, Hadjirin, Nazreen, Timmerman, Arjen J, Wagenaar, Jaap A, Klunder, Heleen M, Fitzgerald, J Ross, Zadoks, Ruth, Paterson, Gavin K, Torres, Carmen, Waller, Andrew S, Loeffler, Anette, Loncaric, Igor, Hoet, Armando E, Bergström, Karin, De Martino, Luisa, Pomba, Constança, de Lencastre, Hermínia, Ben Slama, Karim, Gharsa, Haythem, Richardson, Emily J, Chilvers, Edwin R, de Haas, Carla, van Kessel, Kok, van Strijp, Jos A G, Harrison, Ewan M, Holmes, Mark A, dFAH I&I, dFAH AVR, dI&I I&I-4, Koop, Gerrit, Vrieling, Manouk, Storisteanu, Daniel M L, Lok, Laurence S C, Monie, Tom, van Wigcheren, Glenn, Raisen, Claire, Ba, Xiaoliang, Gleadall, Nicholas, Hadjirin, Nazreen, Timmerman, Arjen J, Wagenaar, Jaap A, Klunder, Heleen M, Fitzgerald, J Ross, Zadoks, Ruth, Paterson, Gavin K, Torres, Carmen, Waller, Andrew S, Loeffler, Anette, Loncaric, Igor, Hoet, Armando E, Bergström, Karin, De Martino, Luisa, Pomba, Constança, de Lencastre, Hermínia, Ben Slama, Karim, Gharsa, Haythem, Richardson, Emily J, Chilvers, Edwin R, de Haas, Carla, van Kessel, Kok, van Strijp, Jos A G, Harrison, Ewan M, and Holmes, Mark A

Published
2017

19. The TLR2 Antagonist Staphylococcal Superantigen-Like Protein 3 Acts as a Virulence Factor to Promote Bacterial Pathogenicity in vivo

Author

MMB Moleculair A, Infection & Immunity, Koymans, Kirsten J., Goldmann, Oliver, Karlsson, Christofer A.Q., Sital, Wiedjai, Thänert, Robert, Bisschop, Adinda, Vrieling, Manouk, Malmström, Johan, van Kessel, Kok P M, De Haas, Carla J.C., Van Strijp, Jos A.G., Medina, Eva, MMB Moleculair A, Infection & Immunity, Koymans, Kirsten J., Goldmann, Oliver, Karlsson, Christofer A.Q., Sital, Wiedjai, Thänert, Robert, Bisschop, Adinda, Vrieling, Manouk, Malmström, Johan, van Kessel, Kok P M, De Haas, Carla J.C., Van Strijp, Jos A.G., and Medina, Eva

Published
2017

21. Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

Author

MMB, UMC Utrecht, Infection & Immunity, MMB Research line 1, Koop, Gerrit, Vrieling, Manouk, Storisteanu, Daniel M L, Lok, Laurence S C, Monie, Tom, Van Wigcheren, Glenn, Raisen, Claire, Ba, Xiaoliang, Gleadall, Nicholas, Hadjirin, Nazreen, Timmerman, Arjen J., Wagenaar, Jaap A., Klunder, Heleen M., Fitzgerald, J. Ross, Zadoks, Ruth, Paterson, Gavin K., Torres, Carmen, Waller, Andrew S., Loeffler, Anette, Loncaric, Igor, Hoet, Armando E., Bergström, Karin, De Martino, Luisa, Pomba, Constança, De Lencastre, Hermínia, Ben Slama, Karim, Gharsa, Haythem, Richardson, Emily J., Chilvers, Edwin R., Gosselaar-de Haas, CJC, van Kessel, CPM, Van Strijp, Jos A G, Harrison, Ewan M., Holmes, Mark A., MMB, UMC Utrecht, Infection & Immunity, MMB Research line 1, Koop, Gerrit, Vrieling, Manouk, Storisteanu, Daniel M L, Lok, Laurence S C, Monie, Tom, Van Wigcheren, Glenn, Raisen, Claire, Ba, Xiaoliang, Gleadall, Nicholas, Hadjirin, Nazreen, Timmerman, Arjen J., Wagenaar, Jaap A., Klunder, Heleen M., Fitzgerald, J. Ross, Zadoks, Ruth, Paterson, Gavin K., Torres, Carmen, Waller, Andrew S., Loeffler, Anette, Loncaric, Igor, Hoet, Armando E., Bergström, Karin, De Martino, Luisa, Pomba, Constança, De Lencastre, Hermínia, Ben Slama, Karim, Gharsa, Haythem, Richardson, Emily J., Chilvers, Edwin R., Gosselaar-de Haas, CJC, van Kessel, CPM, Van Strijp, Jos A G, Harrison, Ewan M., and Holmes, Mark A.

Published
2017

22. Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

Author

Koop, Gerrit, primary, Vrieling, Manouk, additional, Storisteanu, Daniel M. L., additional, Lok, Laurence S. C., additional, Monie, Tom, additional, van Wigcheren, Glenn, additional, Raisen, Claire, additional, Ba, Xiaoliang, additional, Gleadall, Nicholas, additional, Hadjirin, Nazreen, additional, Timmerman, Arjen J., additional, Wagenaar, Jaap A., additional, Klunder, Heleen M., additional, Fitzgerald, J. Ross, additional, Zadoks, Ruth, additional, Paterson, Gavin K., additional, Torres, Carmen, additional, Waller, Andrew S., additional, Loeffler, Anette, additional, Loncaric, Igor, additional, Hoet, Armando E., additional, Bergström, Karin, additional, De Martino, Luisa, additional, Pomba, Constança, additional, de Lencastre, Hermínia, additional, Ben Slama, Karim, additional, Gharsa, Haythem, additional, Richardson, Emily J., additional, Chilvers, Edwin R., additional, de Haas, Carla, additional, van Kessel, Kok, additional, van Strijp, Jos A. G., additional, Harrison, Ewan M., additional, and Holmes, Mark A., additional

Published
2017
Full Text
View/download PDF

23. The TLR2 Antagonist Staphylococcal Superantigen-Like Protein 3 Acts as a Virulence Factor to Promote Bacterial Pathogenicity in vivo

Author

Koymans, Kirsten J., primary, Goldmann, Oliver, additional, Karlsson, Christofer A.Q., additional, Sital, Wiedjai, additional, Thänert, Robert, additional, Bisschop, Adinda, additional, Vrieling, Manouk, additional, Malmström, Johan, additional, van Kessel, Kok P.M., additional, de Haas, Carla J.C., additional, van Strijp, Jos A.G., additional, and Medina, Eva, additional

Published
2017
Full Text
View/download PDF

24. Immunization routes in cattle impact the levels and neutralizing capacity of antibodies induced against S. aureus immune evasion proteins

Author

Boerhout, Eveline, Vrieling, Manouk, Benedictus, Lindert, Daemen, Ineke, Ravesloot, Lars, Rutten, Victor, Nuijten, Piet, van Strijp, Jos, Koets, Ad, Eisenberg, Susanne, LS Immunologie, Sub Immunologie, I&I RATIA-SIB, Infection & Immunity, LS Immunologie, Sub Immunologie, I&I RATIA-SIB, and Infection & Immunity

Subjects
Staphylococcus aureus, Epidemiology, Bioinformatica & Diermodellen, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Cattle Diseases, Research Support, medicine.disease_cause, Microbiology, 03 medical and health sciences, Immune system, Bacterial Proteins, Immunity, Bio-informatics & Animal models, Journal Article, medicine, Life Science, Animals, Epidemiology, Bio-informatics & Animal models, Non-U.S. Gov't, Mastitis, Bovine, 030304 developmental biology, Epidemiologie, 0303 health sciences, Vaccines, Synthetic, General Veterinary, biology, 030306 microbiology, Research Support, Non-U.S. Gov't, Staphylococcal Vaccines, Staphylococcal Infections, medicine.disease, veterinary(all), Antibodies, Bacterial, 3. Good health, Mastitis, Milk, Immunization, Epidemiologie, Bioinformatica & Diermodellen, Immunology, Humoral immunity, biology.protein, Cattle, Female, Antibody, Adjuvant, Research Article
Abstract

Vaccines against S. aureus bovine mastitis are scarce and show limited protection only. All currently available vaccines are applied via the parenteral (usually intramuscular) route. It is unknown, however, whether this route is the most suitable to specifically increase intramammary immunity to combat S. aureus at the site of infection. Hence, in the present study, immunization via mucosal (intranasal; IN), intramuscular (triangle of the neck; IM), intramammary (IMM) and subcutaneous (suspensory ligament; SC) routes were analyzed for their effects on the quantity of the antibody responses in serum and milk as well as the neutralizing capacity of the antibodies within serum. The experimental vaccine comprised the recombinant S. aureus immune evasion proteins extracellular fibrinogen-binding protein (Efb) and the leukotoxin subunit LukM in an oil-in-water adjuvant combined with a hydrogel and alginate. The highest titer increases for both Efb and LukM specific IgG1 and IgG2 antibody levels in serum and milk were observed following SC/SC immunizations. Furthermore, the harmful effects of Efb and leukotoxin LukMF’ on host-defense were neutralized by serum antibodies in a route-dependent manner. SC/SC immunization resulted in a significant increase in the neutralizing capacity of serum antibodies towards Efb and LukMF’, shown by increased phagocytosis of S. aureus and increased viability of bovine leukocytes. Therefore, a SC immunization route should be considered when aiming to optimize humoral immunity against S. aureus mastitis in cattle. Electronic supplementary material The online version of this article (doi:10.1186/s13567-015-0243-7) contains supplementary material, which is available to authorized users.

Published
2015
Full Text
View/download PDF

25. LukMF′ is the major secreted leukocidin of bovine Staphylococcus aureus and is produced in vivo during bovine mastitis

Author

Vrieling, Manouk, Boerhout, Eveline M., Van Wigcheren, Glenn F., Koymans, Kirsten J., Mols-Vorstermans, Tanja G., de Haas, Carla J C, Aerts, Piet C., Daemen, Ineke J J M, van Kessel, Kok P M, Koets, Ad P., Rutten, Victor P M G, Nuijten, Piet J M, Van Strijp, Jos A G, Benedictus, Lindert, Vrieling, Manouk, Boerhout, Eveline M., Van Wigcheren, Glenn F., Koymans, Kirsten J., Mols-Vorstermans, Tanja G., de Haas, Carla J C, Aerts, Piet C., Daemen, Ineke J J M, van Kessel, Kok P M, Koets, Ad P., Rutten, Victor P M G, Nuijten, Piet J M, Van Strijp, Jos A G, and Benedictus, Lindert

Published
2016

26. LukMF' is the major secreted leukocidin of bovine Staphylococcus aureus and is produced in vivo during bovine mastitis

Author

dFAH I&I, Vrieling, Manouk, Boerhout, Eveline M, van Wigcheren, Glenn F, Koymans, Kirsten J, Mols-Vorstermans, Tanja G, de Haas, Carla J C, Aerts, Piet C, Daemen, Ineke J J M, van Kessel, Kok P M, Koets, Ad P, Rutten, Victor P M G, Nuijten, Piet J M, van Strijp, Jos A G, Benedictus, Lindert, dFAH I&I, Vrieling, Manouk, Boerhout, Eveline M, van Wigcheren, Glenn F, Koymans, Kirsten J, Mols-Vorstermans, Tanja G, de Haas, Carla J C, Aerts, Piet C, Daemen, Ineke J J M, van Kessel, Kok P M, Koets, Ad P, Rutten, Victor P M G, Nuijten, Piet J M, van Strijp, Jos A G, and Benedictus, Lindert

Published
2016

27. LukMF′ is the major secreted leukocidin of bovine Staphylococcus aureus and is produced in vivo during bovine mastitis

Author

MMB, UMC Utrecht, Infection & Immunity, MMB Research line 1, CTI Borghans, Vrieling, Manouk, Boerhout, Eveline M., Van Wigcheren, Glenn F., Koymans, Kirsten J., Mols-Vorstermans, Tanja G., de Haas, Carla J C, Aerts, Piet C., Daemen, Ineke J J M, van Kessel, Kok P M, Koets, Ad P., Rutten, Victor P M G, Nuijten, Piet J M, Van Strijp, Jos A G, Benedictus, Lindert, MMB, UMC Utrecht, Infection & Immunity, MMB Research line 1, CTI Borghans, Vrieling, Manouk, Boerhout, Eveline M., Van Wigcheren, Glenn F., Koymans, Kirsten J., Mols-Vorstermans, Tanja G., de Haas, Carla J C, Aerts, Piet C., Daemen, Ineke J J M, van Kessel, Kok P M, Koets, Ad P., Rutten, Victor P M G, Nuijten, Piet J M, Van Strijp, Jos A G, and Benedictus, Lindert

Published
2016

28. LukMF′ is the major secreted leukocidin of bovine Staphylococcus aureus and is produced in vivo during bovine mastitis

Author

Vrieling, Manouk, primary, Boerhout, Eveline M., additional, van Wigcheren, Glenn F., additional, Koymans, Kirsten J., additional, Mols-Vorstermans, Tanja G., additional, de Haas, Carla J. C., additional, Aerts, Piet C., additional, Daemen, Ineke J. J. M., additional, van Kessel, Kok P. M., additional, Koets, Ad P., additional, Rutten, Victor P. M. G., additional, Nuijten, Piet J.M., additional, van Strijp, Jos A. G., additional, and Benedictus, Lindert, additional

Published
2016
Full Text
View/download PDF

29. Immunization routes in cattle impact the levels and neutralizing capacity of antibodies induced against S. aureus immune evasion proteins

Author

LS Immunologie, Sub Immunologie, I&I RATIA-SIB, Infection & Immunity, Boerhout, Eveline, Vrieling, Manouk, Benedictus, Lindert, Daemen, Ineke, Ravesloot, Lars, Rutten, Victor, Nuijten, Piet, van Strijp, Jos, Koets, Ad, Eisenberg, Susanne, LS Immunologie, Sub Immunologie, I&I RATIA-SIB, Infection & Immunity, Boerhout, Eveline, Vrieling, Manouk, Benedictus, Lindert, Daemen, Ineke, Ravesloot, Lars, Rutten, Victor, Nuijten, Piet, van Strijp, Jos, Koets, Ad, and Eisenberg, Susanne

Published
2015

30. Immunization routes in cattle impact the levels and neutralizing capacity of antibodies induced against S. aureus immune evasion proteins

Author

MMB Research line 1, Infection & Immunity, Boerhout, Eveline, Vrieling, Manouk, Benedictus, Lindert, Daemen, Ineke, Ravesloot, Lars, Rutten, Victor, Nuijten, Piet, Van Strijp, Jos, Koets, Ad, Eisenberg, Susanne, MMB Research line 1, Infection & Immunity, Boerhout, Eveline, Vrieling, Manouk, Benedictus, Lindert, Daemen, Ineke, Ravesloot, Lars, Rutten, Victor, Nuijten, Piet, Van Strijp, Jos, Koets, Ad, and Eisenberg, Susanne

Published
2015

33. Identification of a staphylococcal complement inhibitor with broad host specificity in equid Staphylococcus aureus strains

Author

de Jong, Nienke W.M., Vrieling, Manouk, Garcia, Brandon L, Koop, Gerrit, Brettmann, Matt, Aerts, Piet C, Ruyken, Maartje, van Strijp, Jos A G, Holmes, Mark A., Harrison, Ewan M., Geisbrecht, Brian V, Rooijakkers, Suzan H. M., Sub Biomolecular Imaging, LS Immunologie, LS GZ Landbouwhuisdieren, dFAH I&I, dFAH AVR, Sub Biomolecular Imaging, LS Immunologie, LS GZ Landbouwhuisdieren, dFAH I&I, and dFAH AVR

Subjects
0301 basic medicine, Host–pathogen interaction, 030106 microbiology, SCIN, Virulence, microbial pathogenesis, Biology, host-pathogen interaction, medicine.disease_cause, Biochemistry, Microbiology, 03 medical and health sciences, Complement inhibitor, medicine, Life Science, innate immunity, Molecular Biology, Host adaptation, Prophage, complement system, equine, immune evasion, Equine, Immune evasion, Cell Biology, host adaptation, C3-convertase, 3. Good health, Complement system, Staphylococcus aureus (S. aureus), 030104 developmental biology, Staphylococcus aureus
Abstract

Staphylococcus aureus is a versatile pathogen capable of causing a broad range of diseases in many different hosts. S. aureus can adapt to its host through modification of its genome (e.g. by acquisition and exchange of mobile genetic elements that encode host-specific virulence factors). Recently, the prophage φSaeq1 was discovered in S. aureus strains from six different clonal lineages almost exclusively isolated from equids. Within this phage, we discovered a novel variant of staphylococcal complement inhibitor (SCIN), a secreted protein that interferes with activation of the human complement system, an important line of host defense. We here show that this equine variant of SCIN, eqSCIN, is a potent blocker of equine complement system activation and subsequent phagocytosis of bacteria by phagocytes. Mechanistic studies indicate that eqSCIN blocks equine complement activation by specific inhibition of the C3 convertase enzyme (C3bBb). Whereas SCIN-A from human S. aureus isolates exclusively inhibits human complement, eqSCIN represents the first animal-adapted SCIN variant that functions in a broader range of hosts (horses, humans, and pigs). Binding analyses suggest that the human-specific activity of SCIN-A is related to amino acid differences on both sides of the SCIN-C3b interface. These data suggest that modification of this phage-encoded complement inhibitor plays a role in the host adaptation of S. aureus and are important to understand how this pathogen transfers between different hosts.

Full Text
View/download PDF

34. Population Analysis of Staphylococcus aureusReveals a Cryptic, Highly Prevalent Superantigen SElW That Contributes to the Pathogenesis of Bacteremia

Author

Vrieling, Manouk, Tuffs, Stephen W., Yebra, Gonzalo, van Smoorenburg, Marleen Y., Alves, Joana, Pickering, Amy C., Park, Joo Youn, Park, Nogi, Heinrichs, David E., Benedictus, Lindert, Connelley, Timothy, Seo, Keun Seok, McCormick, John K., and Fitzgerald, J. Ross

Abstract

Staphylococcus aureusis an important human and animal pathogen associated with an array of diseases, including life-threatening necrotizing pneumonia and infective endocarditis. The success of S. aureusas a pathogen has been linked in part to its ability to manipulate the host immune response through the secretion of toxins and immune evasion molecules. The staphylococcal superantigens (SAgs) have been studied for decades, but their role in S. aureuspathogenesis is not well understood, and an appreciation for how SAgs manipulate the host immune response to promote infection may be crucial for the development of novel intervention strategies. Here, we characterized a widely prevalent, previously cryptic, staphylococcal SAg, SElW, that contributes to the severity of S. aureusinfections caused by an important epidemic clone of S. aureusCC398. Our findings add to the understanding of staphylococcal SAg diversity and function and provide new insights into the capacity of S. aureusto cause disease.

Published
2020
Full Text
View/download PDF

35. Gene exchange drives the ecological success of a multi-host bacterial pathogen

Author

Richardson, Emily J, Bacigalupe, Rodrigo, Harrison, Ewan M, Weinert, Lucy A, Lycett, Samantha, Vrieling, Manouk, Robb, Kirsty, Hoskisson, Paul A, Holden, Matthew TG, Feil, Edward J, Paterson, Gavin K, Tong, Steven YC, Shittu, Adebayo, Van Wamel, Willem, Aanensen, David M, Parkhill, Julian, Peacock, Sharon J, Corander, Jukka, Holmes, Mark, and Fitzgerald, J Ross

Subjects
2. Zero hunger, Staphylococcus aureus, Livestock, Gene Transfer, Horizontal, Staphylococcal Infections, 3. Good health, Evolution, Molecular, Genes, Bacterial, Host-Pathogen Interactions, Animals, Humans, Cattle, Genome, Bacterial, Phylogeny, Pseudogenes, Genome-Wide Association Study
Abstract

The capacity for some pathogens to jump into different host-species populations is a major threat to public health and food security. Staphylococcus aureus is a multi-host bacterial pathogen responsible for important human and livestock diseases. Here, using a population-genomic approach, we identify humans as a major hub for ancient and recent S. aureus host-switching events linked to the emergence of endemic livestock strains, and cows as the main animal reservoir for the emergence of human epidemic clones. Such host-species transitions are associated with horizontal acquisition of genetic elements from host-specific gene pools conferring traits required for survival in the new host-niche. Importantly, genes associated with antimicrobial resistance are unevenly distributed among human and animal hosts, reflecting distinct antibiotic usage practices in medicine and agriculture. In addition to gene acquisition, genetic diversification has occurred in pathways associated with nutrient acquisition, implying metabolic remodelling after a host switch in response to distinct nutrient availability. For example, S. aureus from dairy cattle exhibit enhanced utilization of lactose-a major source of carbohydrate in bovine milk. Overall, our findings highlight the influence of human activities on the multi-host ecology of a major bacterial pathogen, underpinned by horizontal gene transfer and core genome diversification.

36. Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

Author

Andrew S. Waller, Igor Loncaric, Jaap A. Wagenaar, Luisa De Martino, Haythem Gharsa, Jos A. G. van Strijp, Emily J. Richardson, Nicholas Gleadall, Carmen Torres, Laurence Si Lok, Arjen J. Timmerman, Armando E. Hoet, Ewan M. Harrison, Tom P. Monie, Constança Pomba, Carla J. C. de Haas, Xiaoliang Ba, Anette Loeffler, Gerrit Koop, Mark A. Holmes, Edwin R. Chilvers, Gavin K. Paterson, Heleen M Klunder, Hermínia de Lencastre, Ruth N. Zadoks, Manouk Vrieling, Claire Raisen, Karin Bergström, J. Ross Fitzgerald, Kok P. M. van Kessel, Glenn F van Wigcheren, Daniel M. L. Storisteanu, Nazreen F. Hadjirin, Karim Ben Slama, Lok, Laurence [0000-0002-9364-4213], Monie, Tom [0000-0003-4097-1680], Ba, Xiaoliang [0000-0002-3882-3585], Chilvers, Edwin [0000-0002-4230-9677], Harrison, Ewan [0000-0003-2720-0507], Holmes, Mark [0000-0002-5454-1625], Apollo - University of Cambridge Repository, Koop, Gerrit, Vrieling, Manouk, Storisteanu, Daniel M. L., Lok, Laurence S. C., Monie, Tom, Van Wigcheren, Glenn, Raisen, Claire, Ba, Xiaoliang, Gleadall, Nichola, Hadjirin, Nazreen, Timmerman, Arjen J., Wagenaar, Jaap A., Klunder, Heleen M., Fitzgerald, J. Ro, Zadoks, Ruth, Paterson, Gavin K., Torres, Carmen, Waller, Andrew S., Loeffler, Anette, Loncaric, Igor, Hoet, Armando E., Bergström, Karin, DE MARTINO, Luisa, Pomba, Constança, De Lencastre, Hermínia, Ben Slama, Karim, Gharsa, Haythem, Richardson, Emily J., Chilvers, Edwin R., De Haas, Carla, Van Kessel, Kok, Van Strijp, Jos A. G., Harrison, Ewan M., Holmes, Mark A., dFAH I&I, dFAH AVR, and dI&I I&I-4

Subjects
0301 basic medicine, Neutrophils, Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2], Cell, HUMAN C5A RECEPTORS, Leukocidin, Host tropism, PROTEIN, Plasma protein binding, medicine.disease_cause, LYMPHOCYTES, Receptors, Interleukin-8B, Leukocidins, BINDING, Gene Order, CHEMOKINE RECEPTORS, GAMMA-HEMOLYSIN, Receptor, Phylogeny, Multidisciplinary, Bacteriologie, Bacteriology, Host Pathogen Interaction & Diagnostics, Staphylococcal Infections, Multidisciplinary Sciences, medicine.anatomical_structure, Staphylococcus aureus, Science & Technology - Other Topics, BOVINE, Pathogens, Protein Binding, Cell Survival, 030106 microbiology, Bacterial Toxins, Phage biology, Biology, Staphylococcal infections, LEUKOTOXIN, Article, Host Specificity, Microbiology, 03 medical and health sciences, PANTON-VALENTINE LEUCOCIDIN, Journal Article, medicine, Life Science, Animals, Humans, Horses, General, Prophage, Host Pathogen Interaction & Diagnostics, Science & Technology, Bacteriology, medicine.disease, Host Pathogen Interactie & Diagnostiek, 030104 developmental biology, Bacteriologie, Host Pathogen Interactie & Diagnostiek, Cattle, Horse Diseases
Abstract

Contains fulltext : 177770.pdf (Publisher’s version ) (Open Access) Bicomponent pore-forming leukocidins are a family of potent toxins secreted by Staphylococcus aureus, which target white blood cells preferentially and consist of an S- and an F-component. The S-component recognizes a receptor on the host cell, enabling high-affinity binding to the cell surface, after which the toxins form a pore that penetrates the cell lipid bilayer. Until now, six different leukocidins have been described, some of which are host and cell specific. Here, we identify and characterise a novel S. aureus leukocidin; LukPQ. LukPQ is encoded on a 45 kb prophage (PhiSaeq1) found in six different clonal lineages, almost exclusively in strains cultured from equids. We show that LukPQ is a potent and specific killer of equine neutrophils and identify equine-CXCRA and CXCR2 as its target receptors. Although the S-component (LukP) is highly similar to the S-component of LukED, the species specificity of LukPQ and LukED differs. By forming non-canonical toxin pairs, we identify that the F-component contributes to the observed host tropism of LukPQ, thereby challenging the current paradigm that leukocidin specificity is driven solely by the S-component.

Published
2017
Full Text
View/download PDF

37. H5N1 clade 2.3.4.4b avian influenza viruses replicate in differentiated bovine airway epithelial cells cultured at air-liquid interface.

Author

Bordes L, Gerhards NM, Peters S, van Oort S, Roose M, Dresken R, Venema S, Vrieling M, Engelsma M, van der Poel WHM, and de Swart RL

Subjects
Animals, Cattle, Cells, Cultured, Epithelial Cells virology, Influenza A Virus, H5N1 Subtype genetics, Influenza A Virus, H5N1 Subtype physiology, Influenza A Virus, H5N1 Subtype isolation & purification, Virus Replication
Abstract

Highly pathogenic avian influenza (HPAI) H5N1 viruses are responsible for disease outbreaks in wild birds and poultry, resulting in devastating losses to the poultry sector. Since 2020, an increasing number of outbreaks of HPAI H5N1 was seen in wild birds. Infections in mammals have become more common, in most cases in carnivores after direct contact with infected birds. Although ruminants were previously not considered a host species for HPAI viruses, in March 2024 multiple outbreaks of HPAI H5N1 were detected in goats and cattle in the United States. Here, we have used primary bronchus-derived well-differentiated bovine airway epithelial cells (WD-AECs) cultured at air-liquid interface to assess the susceptibility and permissiveness of bovine epithelial cells to infection with European H5N1 virus isolates. We inoculated bovine WD-AECs with three low-passage HPAI clade 2.3.4.4b H5N1 virus isolates and detected rapid increases in viral genome loads and infectious virus during the first 24 h post-inoculation, without substantial cytopathogenic effects. Three days post-inoculation infected cells were still detectable by immunofluorescent staining. These data indicate that multiple lineages of HPAI H5N1 may have the propensity to infect the respiratory tract of cattle and support extension of avian influenza surveillance efforts to ruminants. Furthermore, this study underscores the benefit of WD-AEC cultures for pandemic preparedness by providing a rapid and animal-free assessment of the host range of an emerging pathogen.

Published
2024
Full Text
View/download PDF

38. Immunization routes in cattle impact the levels and neutralizing capacity of antibodies induced against S. aureus immune evasion proteins.

Author

Boerhout E, Vrieling M, Benedictus L, Daemen I, Ravesloot L, Rutten V, Nuijten P, van Strijp J, Koets A, and Eisenberg S

Subjects
Animals, Antibodies, Bacterial blood, Bacterial Proteins immunology, Cattle, Cattle Diseases immunology, Cattle Diseases microbiology, Female, Mastitis, Bovine immunology, Mastitis, Bovine microbiology, Mastitis, Bovine prevention & control, Milk chemistry, Staphylococcal Infections immunology, Staphylococcal Infections microbiology, Staphylococcal Infections prevention & control, Vaccines, Synthetic immunology, Antibodies, Bacterial metabolism, Cattle Diseases prevention & control, Immunization methods, Staphylococcal Infections veterinary, Staphylococcal Vaccines immunology, Staphylococcus aureus immunology
Abstract

Vaccines against S. aureus bovine mastitis are scarce and show limited protection only. All currently available vaccines are applied via the parenteral (usually intramuscular) route. It is unknown, however, whether this route is the most suitable to specifically increase intramammary immunity to combat S. aureus at the site of infection. Hence, in the present study, immunization via mucosal (intranasal; IN), intramuscular (triangle of the neck; IM), intramammary (IMM) and subcutaneous (suspensory ligament; SC) routes were analyzed for their effects on the quantity of the antibody responses in serum and milk as well as the neutralizing capacity of the antibodies within serum. The experimental vaccine comprised the recombinant S. aureus immune evasion proteins extracellular fibrinogen-binding protein (Efb) and the leukotoxin subunit LukM in an oil-in-water adjuvant combined with a hydrogel and alginate. The highest titer increases for both Efb and LukM specific IgG1 and IgG2 antibody levels in serum and milk were observed following SC/SC immunizations. Furthermore, the harmful effects of Efb and leukotoxin LukMF' on host-defense were neutralized by serum antibodies in a route-dependent manner. SC/SC immunization resulted in a significant increase in the neutralizing capacity of serum antibodies towards Efb and LukMF', shown by increased phagocytosis of S. aureus and increased viability of bovine leukocytes. Therefore, a SC immunization route should be considered when aiming to optimize humoral immunity against S. aureus mastitis in cattle.

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results