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28 results on '"Vrenna, Gianluca"'

1. First Evidence of Streptococcus pyogenes M1UK Clone in Pediatric Invasive Infections in Italy by Molecular Surveillance

Author

Vrenna, Gianluca, Rossitto, Martina, Agosta, Marilena, Cortazzo, Venere, Fox, Valeria, De Luca, Maia, Lancella, Laura, Gargiullo, Livia, Granaglia, Annarita, Fini, Vanessa, Yu La Rosa, Katia, Argentieri, Marta, Pansani, Laura, Sisto, Annamaria, Raponi, Massimiliano, Villani, Alberto, Perno, Carlo Federico, and Bernaschi, Paola

Published
2024
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2. The Challenging Life of Mutators: How Pseudomonas aeruginosa Survives between Persistence and Evolution in Cystic Fibrosis Lung.

Author

Rossitto, Martina, Fox, Valeria, Vrenna, Gianluca, Tuccio Guarna Assanti, Vanessa, Essa, Nour, Lepanto, Maria Stefania, Raimondi, Serena, Agosta, Marilena, Cortazzo, Venere, Fini, Vanessa, Granaglia, Annarita, Montemitro, Enza, Cutrera, Renato, Perno, Carlo Federico, and Bernaschi, Paola

Abstract

Cystic fibrosis (CF) is a life-threatening genetic disease characterised by chronic lung infections sustained by opportunistic pathogens such as Pseudomonas aeruginosa. During the chronic long-lasting lung infections, P. aeruginosa adapts to the host environment. Hypermutability, mainly due to defects in the DNA repair system, resulting in an increased spontaneous mutation rate, represents a way to boost the rapid adaptation frequently encountered in CF P. aeruginosa isolates. We selected 609 isolates from 51 patients with CF chronically colonised by P. aeruginosa to study, by full-length genome sequencing, the longitudinal evolution of the bacterium. We recovered at least one hypermutable (mutator) isolate in 57% of patients. By combining genomic information and phenotypic analyses, we followed the evolutionary pathways of the P. aeruginosa mutator strains, identifying their contribution to multi-drug resistance and the emergence of new sub-lineages. By implementing patient clinical data, we observed that mutators preferentially follow a specific evolutionary trajectory in patients with a negative clinical outcome and that maintenance antibiotic polytherapy, based on alternating molecules, apparently reduces the occurrence of hypermutability. Finally, we draw attention to the possibility that modulator-induced changes in the pulmonary environment may be associated with the onset of hypermutability. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Identification of the blaOXA-23 Gene in the First Mucoid XDR Acinetobacter baumannii Isolated from a Patient with Cystic Fibrosis

Author

Rossitto, Martina, primary, Vrenna, Gianluca, additional, Tuccio Guarna Assanti, Vanessa, additional, Essa, Nour, additional, De Santis, Maria Luisa, additional, Granaglia, Annarita, additional, Fini, Vanessa, additional, Costabile, Valentino, additional, Onori, Manuela, additional, Cristiani, Luca, additional, Boni, Alessandra, additional, Cutrera, Renato, additional, Perno, Carlo Federico, additional, and Bernaschi, Paola, additional

Published
2023
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5. First Case of VIM-1-like-Producing Pseudomonas putida Bacteremia in an Oncohematological Pediatric Patient in Italy

Author

Cortazzo, Venere, primary, Agosta, Marilena, additional, Gaspari, Stefania, additional, Vrenna, Gianluca, additional, Lucignano, Barbara, additional, Onori, Manuela, additional, Di Ruscio, Valentina, additional, Mancinelli, Livia, additional, Domo, Danielle, additional, Perno, Carlo Federico, additional, and Bernaschi, Paola, additional

Published
2023
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7. Virulence of Pseudomonas aeruginosa in Cystic Fibrosis: Relationships between Normoxia and Anoxia Lifestyle.

Author

Papa, Rosanna, Imperlini, Esther, Trecca, Marika, Paris, Irene, Vrenna, Gianluca, Artini, Marco, and Selan, Laura

Subjects
PSEUDOMONADACEAE, CYSTIC fibrosis, ACHROMOBACTER, PSEUDOMONAS aeruginosa, HYPOXEMIA, DRUG resistance in bacteria, OSMOTIC pressure, MULTIDRUG tolerance (Microbiology)
Abstract

The airways of cystic fibrosis (CF) patients are colonized by many pathogens and the most common is Pseudomonas aeruginosa, an environmental pathogen that is able to infect immunocompromised patients thanks to its ability to develop resistance to conventional antibiotics. Over 12% of all patients colonized by P. aeruginosa harbour multi-drug resistant species. During airway infection in CF, P. aeruginosa adopts various mechanisms to survive in a hostile ecological niche characterized by low oxygen concentration, nutrient limitation and high osmotic pressure. To this end, P. aeruginosa uses a variety of virulence factors including pigment production, biofilm formation, motility and the secretion of toxins and proteases. This study represents the first report that systematically analyzes the differences in virulence features, in normoxia and anoxia, of clinical P. aeruginosa isolated from CF patients, characterized by multi- or pan-drug antibiotic resistance compared to antibiotic sensitive strains. The virulence features, such as biofilm formation, protease secretion and motility, are highly diversified in anaerobiosis, which reflects the condition of chronic CF infection. These findings may contribute to the understanding of the real-world lifestyle of pathogens isolated during disease progression in each particular patient and to assist in the design of therapeutic protocols for personalized medicine. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Identification of the bla OXA-23 Gene in the First Mucoid XDR Acinetobacter baumannii Isolated from a Patient with Cystic Fibrosis.

Author

Rossitto, Martina, Vrenna, Gianluca, Tuccio Guarna Assanti, Vanessa, Essa, Nour, De Santis, Maria Luisa, Granaglia, Annarita, Fini, Vanessa, Costabile, Valentino, Onori, Manuela, Cristiani, Luca, Boni, Alessandra, Cutrera, Renato, Perno, Carlo Federico, and Bernaschi, Paola

Subjects
*ACINETOBACTER baumannii, *CYSTIC fibrosis, *KLEBSIELLA infections, *CROSS infection, *CHILDREN'S hospitals, *LUNG infections, *DRUG resistance in microorganisms
Abstract

Acinetobacter baumannii is one of the pathogens most involved in health care-associated infections in recent decades. Known for its ability to accumulate several antimicrobial resistance mechanisms, it possesses the oxacillinase blaoxa-23, a carbapenemase now endemic in Italy. Acinetobacter species are not frequently observed in patients with cystic fibrosis, and multidrug-resistant A. baumannii is a rare event in these patients. Non-mucoid A. baumannii carrying the blaoxa-23 gene has been sporadically detected. Here, we describe the methods used to detect blaoxa-23 in the first established case of pulmonary infection via a mucoid strain of A. baumannii producing carbapenemase in a 24-year-old cystic fibrosis patient admitted to Bambino Gesù Children's Hospital in Rome, Italy. This strain, which exhibited an extensively drug-resistant antibiotype, also showed a great ability to further increase its resistance in a short time. [ABSTRACT FROM AUTHOR]

Published
2023
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10. Essential Oils Biofilm Modulation Activity and Machine Learning Analysis on Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients

Author

Artini, Marco, primary, Papa, Rosanna, additional, Sapienza, Filippo, additional, Božović, Mijat, additional, Vrenna, Gianluca, additional, Tuccio Guarna Assanti, Vanessa, additional, Sabatino, Manuela, additional, Garzoli, Stefania, additional, Fiscarelli, Ersilia Vita, additional, Ragno, Rino, additional, and Selan, Laura, additional

Published
2022
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11. Acinetobacter baumannii Isolates from COVID-19 Patients in a Hospital Intensive Care Unit: Molecular Typing and Risk Factors

Author

Ceparano, Mariateresa, primary, Baccolini, Valentina, additional, Migliara, Giuseppe, additional, Isonne, Claudia, additional, Renzi, Erika, additional, Tufi, Daniela, additional, De Vito, Corrado, additional, De Giusti, Maria, additional, Trancassini, Maria, additional, Alessandri, Francesco, additional, Ceccarelli, Giancarlo, additional, Pugliese, Francesco, additional, Villari, Paolo, additional, Angiulli, Maria, additional, Battellito, Stefania, additional, Bellini, Arianna, additional, Bongiovanni, Andrea, additional, Caivano, Lucilla, additional, Castellani, Marta, additional, Coletti, Monica, additional, Cottarelli, Alessia, additional, D’Agostino, Ludovica, additional, De Giorgi, Andrea, additional, De Marchi, Chiara, additional, Germani, Irma, additional, Giannini, Dara, additional, Mazzeo, Elisa, additional, Orlandi, Shadi, additional, Piattoli, Matteo, additional, Ricci, Eleonora, additional, Siena, Leonardo Maria, additional, Territo, Alessandro, additional, Vrenna, Gianluca, additional, Zanni, Stefano, additional, and Marzuillo, Carolina, additional

Published
2022
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12. A Shaving Proteomic Approach to Unveil Surface Proteins Modulation of Multi-Drug Resistant Pseudomonas aeruginosa Strains Isolated From Cystic Fibrosis Patients

Author

Montemari, Anna Lisa, primary, Marzano, Valeria, additional, Essa, Nour, additional, Levi Mortera, Stefano, additional, Rossitto, Martina, additional, Gardini, Simone, additional, Selan, Laura, additional, Vrenna, Gianluca, additional, Onetti Muda, Andrea, additional, Putignani, Lorenza, additional, and Fiscarelli, Ersilia Vita, additional

Published
2022
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14. Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients

Author

Papa, Rosanna, primary, Vrenna, Gianluca, additional, D’Angelo, Caterina, additional, Casillo, Angela, additional, Relucenti, Michela, additional, Donfrancesco, Orlando, additional, Corsaro, Maria Michela, additional, Fiscarelli, Ersilia Vita, additional, Tuccio Guarna Assanti, Vanessa, additional, Tutino, Maria Luisa, additional, Parrilli, Ermenegilda, additional, Artini, Marco, additional, and Selan, Laura, additional

Published
2021
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15. Essential Oils Biofilm Modulation Activity, Chemical and Machine Learning Analysis—Application on Staphylococcus aureus Isolates from Cystic Fibrosis Patients

Author

Papa, Rosanna, primary, Garzoli, Stefania, additional, Vrenna, Gianluca, additional, Sabatino, Manuela, additional, Sapienza, Filippo, additional, Relucenti, Michela, additional, Donfrancesco, Orlando, additional, Fiscarelli, Ersilia, additional, Artini, Marco, additional, Selan, Laura, additional, and Ragno, Rino, additional

Published
2020
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16. Characterization of Scardovia wiggsiae Biofilm by Original Scanning Electron Microscopy Protocol

Author

Bossù, Maurizio, primary, Selan, Laura, additional, Artini, Marco, additional, Relucenti, Michela, additional, Familiari, Giuseppe, additional, Papa, Rosanna, additional, Vrenna, Gianluca, additional, Spigaglia, Patrizia, additional, Barbanti, Fabrizio, additional, Salucci, Alessandro, additional, Di Giorgio, Gianni, additional, Rau, Julietta V., additional, and Polimeni, Antonella, additional

Published
2020
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18. Machine Learning Analyses on Data including Essential Oil Chemical Composition and In Vitro Experimental Antibiofilm Activities against Staphylococcus Species

Author

Patsilinakos, Alexandros, primary, Artini, Marco, additional, Papa, Rosanna, additional, Sabatino, Manuela, additional, Božović, Mijat, additional, Garzoli, Stefania, additional, Vrenna, Gianluca, additional, Buzzi, Raissa, additional, Manfredini, Stefano, additional, Selan, Laura, additional, and Ragno, Rino, additional

Published
2019
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21. Antimicrobial and Antibiofilm Activity and Machine Learning Classification Analysis of Essential Oils from Different Mediterranean Plants against Pseudomonas aeruginosa

Author

Artini, Marco, primary, Patsilinakos, Alexandros, additional, Papa, Rosanna, additional, Božović, Mijat, additional, Sabatino, Manuela, additional, Garzoli, Stefania, additional, Vrenna, Gianluca, additional, Tilotta, Marco, additional, Pepi, Federico, additional, Ragno, Rino, additional, and Selan, Laura, additional

Published
2018
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24. Serratiopeptidase reduces the invasion of osteoblasts by Staphylococcus aureus

Author

Carla Renata Arciola, Marco Artini, Laura Selan, Davide Campoccia, Evaristo Ettorre, Lucio Montanaro, Gianluca Vrenna, Rosanna Papa, A. Cellini, Angela Ermocida, Selan, Laura, Papa, Rosanna, Ermocida, Angela, Cellini, Andrea, Ettorre, Evaristo, Vrenna, Gianluca, Campoccia, Davide, Montanaro, Lucio, Arciola, Carla Renata, and Artini, Marco

Subjects
0301 basic medicine, Staphylococcus aureus, Chemokine, Osteolysis, Cell Survival, 030106 microbiology, Immunology, Protective Agents, medicine.disease_cause, Cell Line, Microbiology, 03 medical and health sciences, Staphylococcu, Serratiopeptidase, Bone cell, medicine, Humans, Immunology and Allergy, Viability assay, Letters to the Editor, serratiopeptidase, Chemokine CCL2, Cell Proliferation, Pharmacology, Osteoblasts, biology, Osteoblast, Staphylococcal Infections, medicine.disease, implant infection, 030104 developmental biology, medicine.anatomical_structure, inflammation, Chemokine secretion, staphylococcus, osteoblast, biology.protein, Peptide Hydrolases
Abstract

Finding new strategies to counteract periprosthetic infection and implant failure is a main target in orthopedics. Staphylococcus aureus, the leading etiologic agent of orthopedic implant infections, is able to enter and kill osteoblasts, to stimulate pro-inflammatory chemokine secretion, to recruit osteoclasts, and to cause inflammatory osteolysis. Moreover, by entering eukaryotic cells, staphylococci hide from the host immune defenses and shelter from the extracellular antibiotics. Thus, infection persists, inflammation thrives, and a highly destructive osteomyelitis occurs around the implant. The ability of serratiopeptidase (SPEP), a metalloprotease by Serratia marcescens, to control S. aureus invasion of osteoblastic MG-63 cells and pro-inflammatory chemokine MCP-1 secretion was evaluated. Human osteoblast cells were infected with staphylococcal strains in the presence and in the absence of SPEP. Cell proliferation and cell viability were also evaluated. The release of pro-inflammatory chemokine MCP-1 was evaluated after the exposure of the osteoblast cells to staphylococcal strains. The significance of the differences in the results of each test and the relative control values was determined with Student’s t-test. SPEP impairs their invasiveness into osteoblasts, without affecting the viability and proliferation of bone cells, and tones down their production of MCP-1. We recognize SPEP as a potential tool against S. aureus bone infection and destruction.

Published
2017

25. Pentadecanal inspired molecules as new anti-biofilm agents against Staphylococcus epidermidis

Author

Laura Selan, Paola Imbimbo, Ermenegilda Parrilli, Annarita Ricciardelli, Angela Casillo, Daria Maria Monti, Marco Artini, Maria Michela Corsaro, Maria Luisa Tutino, Gianluca Vrenna, Rosanna Papa, Ricciardelli, Annarita, Casillo, Angela, Papa, Rosanna, Monti, Daria Maria, Imbimbo, Paola, Vrenna, Gianluca, Artini, Marco, Selan, Laura, Corsaro, Maria Michela, Tutino, Maria Luisa, and Parrilli, Ermenegilda

Subjects
0301 basic medicine, medicine.drug_class, Antibiotics, Anti-biofilm, pentadecanal, pseudoalteromonas haloplanktis tac125, vancomycin, pentadecanoic acid, pentadecanoic acid methyl ester, 1,1-dimethoxypentadecane, Microbial Sensitivity Tests, Aquatic Science, Applied Microbiology and Biotechnology, Pseudoalteromonas haloplanktis, Microbiology, 03 medical and health sciences, Staphylococcus epidermidis, Vancomycin, medicine, Humans, Cytotoxicity, Water Science and Technology, Aldehydes, biology, Chemistry, TAC125, Biofilm, Drug Synergism, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Antimicrobial, Anti-Bacterial Agents, 030104 developmental biology, Biofilms, Bacteria, medicine.drug, Disinfectants
Abstract

Staphylococcus epidermidis, a harmless human skin colonizer, is a significant nosocomial pathogen in predisposed hosts because of its capability to form a biofilm on indwelling medical devices. In a recent paper, the purification and identification of the pentadecanal produced by the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125, able to impair S. epidermidis biofilm formation, were reported. Here the authors report on the chemical synthesis of pentadecanal derivatives, their anti-biofilm activity on S. epidermidis, and their action in combination with antibiotics. The results clearly indicate that the pentadecanal derivatives were able to prevent, to a different extent, biofilm formation and that pentadecanoic acid positively modulated the antimicrobial activity of the vancomycin. The cytotoxicity of these new anti-biofilm molecules was tested on two different immortalized eukaryotic cell lines in view of their potential applications.

Published
2019

26. Hydrophobin coating prevents Staphylococcus epidermidis biofilm formation on different surfaces

Author

Principia Dardano, Paola Cicatiello, Marco Artini, Paola Giardina, Annarita Ricciardelli, Marco Tilotta, Laura Selan, Ermenegilda Parrilli, Maria Luisa Tutino, Gianluca Vrenna, Rosanna Papa, Artini, Marco, Cicatiello, Paola, Ricciardelli, Annarita, Papa, Rosanna, Selan, Laura, Dardano, Principia, Tilotta, Marco, Vrenna, Gianluca, Tutino, MARIA LUISA, Giardina, Paola, and Parrilli, Ermenegilda

Subjects
0301 basic medicine, Confocal laser scanning microscope, Hydrophobin, Surface Properties, surface colonization, 030106 microbiology, protein self-assembly, fungi, biofilm inhibition, confocal laser scanning microscopy, cdc biofilm reactor, Aquatic Science, engineering.material, Microscopy, Atomic Force, Pleurotus, Applied Microbiology and Biotechnology, Bacterial Adhesion, Microbiology, Fungal Proteins, 03 medical and health sciences, Coating, Staphylococcus epidermidis, Confocal laser scanning microscopy, Water Science and Technology, Microbial Viability, Microscopy, Confocal, biology, Chemistry, Nosocomial pathogens, Biofilm, Adhesion, CDC biofilm reactor, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Protein self-assembly, Acremonium, 030104 developmental biology, Biofilms, engineering, Polystyrenes, Hydrophobic and Hydrophilic Interactions
Abstract

Staphylococcus epidermidis is a significant nosocomial pathogen in predisposed hosts because of its capability of forming a biofilm on indwelling medical devices. The initial stage of biofilm formation has a key role in S. epidermidis abiotic surface colonization. Recently, many strategies have been developed to create new anti-biofilm surfaces able to control bacterial adhesion mechanisms. In this work, the self-assembled amphiphilic layers formed by two fungal hydrophobins (Vmh2 and Pac3) have proven to be able to reduce the biofilm formed by different strains of S. epidermidis on polystyrene surfaces. The reduction in the biofilm thickness on the coated surfaces and the preservation of cell vitality have been demonstrated through confocal laser scanning microscope analysis. Moreover, the anti-biofilm efficiency of the self-assembled layers on different medically relevant materials has also been demonstrated using a CDC biofilm reactor.

Published
2017

27. Virulence of Pseudomonas aeruginosa in Cystic Fibrosis: Relationships between Normoxia and Anoxia Lifestyle.

Author

Papa R, Imperlini E, Trecca M, Paris I, Vrenna G, Artini M, and Selan L

Abstract

The airways of cystic fibrosis (CF) patients are colonized by many pathogens and the most common is Pseudomonas aeruginosa , an environmental pathogen that is able to infect immunocompromised patients thanks to its ability to develop resistance to conventional antibiotics. Over 12% of all patients colonized by P. aeruginosa harbour multi-drug resistant species. During airway infection in CF, P. aeruginosa adopts various mechanisms to survive in a hostile ecological niche characterized by low oxygen concentration, nutrient limitation and high osmotic pressure. To this end, P. aeruginosa uses a variety of virulence factors including pigment production, biofilm formation, motility and the secretion of toxins and proteases. This study represents the first report that systematically analyzes the differences in virulence features, in normoxia and anoxia, of clinical P. aeruginosa isolated from CF patients, characterized by multi- or pan-drug antibiotic resistance compared to antibiotic sensitive strains. The virulence features, such as biofilm formation, protease secretion and motility, are highly diversified in anaerobiosis, which reflects the condition of chronic CF infection. These findings may contribute to the understanding of the real-world lifestyle of pathogens isolated during disease progression in each particular patient and to assist in the design of therapeutic protocols for personalized medicine.

Published
2023
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28. Ultrasound affects minimal inhibitory concentration of ampicillin against methicillin resistant Staphylococcus aureus USA300.

Author

Selan L, Papa R, Barbato G, Scoarughi GL, Vrenna G, and Artini M

Subjects
Anti-Bacterial Agents administration & dosage, Anti-Bacterial Agents pharmacology, Microbial Sensitivity Tests, Ampicillin administration & dosage, Ampicillin pharmacology, Drug Resistance, Bacterial radiation effects, Methicillin-Resistant Staphylococcus aureus, Ultrasonography
Abstract

Antimicrobial resistance is one of the most serious global public health problems. Therefore, novel strategies are needed to counteract bacterial resistance development. The aim of the present study was to enhance the activity of antibiotics to bacteria by using ultrasound. Ultrasound reduced the dosage of ampicillin required to impair bacterial viability.

Published
2019

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