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Environmental pollution (EP), defined as the unfavorable chemical and physical alterations of our surroundings, still represents a relevant issue of this century. In this scenario, several changes may directly or indirectly impact on human health by promoting neoplastic cell transformation due to DNA damage in human tissue. The identification of a specific molecular signature observed in people exposed to environmental contaminants could improve risk calculation of exposition. As regards, DNA methylation is considered an epigenetic modification that could play a pivotal role in the risk calculation of patients under EC exposure. This mechanism, based on the introduction of methyl groups on cytosine’s in CpG islands, is carried out by methyltransferases enzymes able to activate this modification in promotor region by influencing regulation process. Several studies demonstrated that methylation pattern is drastically alterated in subjects under EC exposure. To date, several approaches for the early detection of contaminants in biological fluids are under investigation. Liquid biopsy consists in a peripheral blood withdrawn where several biomolecules [cell-free DNA (ccfDNA), circulating tumor cells (CTCs), circulating cell-free RNA (ccfRNA) and exosomes] may be isolated. Students participate to the validation of a LP assay for the evaluation of environmental determinants with the Lab Residents. They assessed the cost-efficacy and the minimally invasive approach in order to construct a reliable model to be exported for the identification of epigenetic changes in the early stage of contamination process in high risk people. The young students role was also to create an informative schedule in order to explain the role and the potential of Liquid biopsy as a noninvasive tool that could allow the analysis of nucleic biomarkers as circulating cell-free DNA (ccfDNA), a potential stable biomarkers of diverse disease conditions in humans and animals. [ABSTRACT FROM AUTHOR]

Crohn’s disease is a chronic inflammatory bowel disease characterized by transmural inflammation and fistula formation. Perianal fistulas are a common complication of Crohn’s disease and are estimated to affect up to 28% of patients in the first two decades after diagnosis, particularly those with colonic disease and rectal involvement. Complex perianal fistulas in Crohn’s disease are challenging to treat, severely impair patients’ quality of life and cause substantial morbidity. Allogeneic, expanded, adipose-derived stem cells (Cx601) are a promising new therapeutic approach, although their high cost is often discouraging. We aimed at assessing the safety and efficacy of this treatment in patients with complex perianal fistulas in patients with Crohn’s disease. Therefore, we conducted a meta-analysis in order to evaluate this new therapeutic approach before introducing it as standard of care. We examined 8 studies published in the last 10 years, 5 of which were selected for the final analysis. The primary outcome assessed was the combined fistula remission (clinical + imaging) assessed through the clinical examination and MRI, the control being the traditional medical strategies (ie, antibiotics, immunomodulators and anti-tumour necrosis factor agents). We registered a total of 128 events (lack of remission) in the control group vs 94 in the experimental one (Confidence Interval for OR of 0,12-0,31, P<0,001). Our ‘‘in silico’’ assessment is reliable, being the introduction of (Cx601) an innovative and effective treatment. Further insights will be provided by the INSPIRE registry, a multinational postmarketing study whose main objective is the evaluation of the real-world clinical effectiveness and safety of (Cx601) in patients affected by Crohn’s disease with complex perianal fistulas for a duration of 36 months, to which our University ‘‘Federico II’’ is actively taking part. [ABSTRACT FROM AUTHOR]

The Goal 11 <> is crucial in the increasing Europe urbanization context. By altering cities and the surrounding countryside, urban growth is having several consequences on ecosystem and human health. The urban density alludes to the idea of the cities as a ‘sick bodies’, now reemerging because of the recent climatic and health crises. This concept should be contrasted with the idea of the ‘city as a cure’, that can limit the consumption of soil and energy, reducing our ecological burden on the environment. Recently, in Europe many urban development projects have been implemented according to sustainability principles as the Eindhoven demonstration case where, a central square was transformed into a small green oasis. Reforesting, even at the cost of demolishing to create large avenues for green area, is essential for air quality and for intervening on the climate. To intervene on abandoned urban canals by creating new waterways may redevelop entire areas and affect traffic and temperature. To contain the negative impact of the built space on the environment is in fact aimed at increasing the green space. However, recent studies demonstrate the criticalities of the fragmentation of landscapeto analyze the link between urban form and landscape heterogeneity to establish renewed relationships between the forms of architecture and the natural void and to devise urban projects to control building density and population. In particular, to develop models for redeveloping the suburbs by building new urban parts from scratch, with a view to urban polycentrism. The recomposition of ecological corridors and of the municipal, provincial and regional environmental network may connect to the great ‘green lungs’ of natural parks and to areas with a high degree of naturalness in a progressive succession of habitats to mitigate ever new forms of overall mutations of natural and anthropised environments. [ABSTRACT FROM AUTHOR]

Background: Acinetobacter baumannii has become an increasingly important nosocomial pathogen, particularly in intensive care units (ICUs). The aim of this investigation was to study the molecular epidemiology of A baumanii in a university hospital in Italy., Methods: All A baumanii isolates were collected and typed with phenotypic and genotypic methods during a 7-month period. A 1-year prospective surveillance of ICU-acquired infections was performed by using the National Nosocomial Infections Surveillance methodology., Results: A baumanni accounted for 28.4% of all infections and 46.7% of all pneumonia acquired in the ICU, with a nosocomial infection rate of 12.4% or 8 infections per 1000 patient-days. Risk factors for A baumannii acquisition in the ICU were mechanical ventilation and previous use of broad-spectrum antibiotics, whereas administration of carbapenems showed a significant protective effect. Pulsed-field gel electrophoresis of genomic Apa I digests identified at least 5 outbreaks in the ICU caused by 5 different clones, one replacing the other in a well-defined temporal order., Conclusions: Whereas the sequential temporal cluster of epidemic clones in the ICU is intriguing and requires further research, the clear evidence of cross-contamination of A baumannii isolates involved with infections in the ICU demands extensive preventive efforts.