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Background: Steatotic liver disease (SLD) prevalence is rising worldwide, linked to insulin resistance and obesity. SLD prevalence can surpass 10% even among those with normal weight. In Lao People's Democratic Republic (Lao PDR), where Opisthorchis viverrini (OV) trematode infection and type 2 diabetes mellitus (T2DM) are common, infection related liver morbidity such as cholangiocarcinoma (CCA) is high, but data on SLD prevalence is lacking. The objective of this study was to estimate the prevalence and explore determinants of SLD in rural southern Lao PDR for lean and non-lean populations. Method: A cross-sectional community-based study assessed SLD prevalence using abdominal ultrasonography (US). Factors investigated for association with SLD were identified by interview, serological tests (Hepatitis B surface antigen (HBsAg); lipids and HbA1c), anthropometrical measurements, and parasitological assessments (OV infection). Uni- and multivariable logistic regression analyses with SLD as endpoint were conducted separately for lean (body mass index (BMI) <23.0kg/m²) and non-lean (BMI ≥ 23.0kg/m²) participants. Result: 2,826 participants were included. SLD prevalence was 27.1% (95% confidence interval (95% CI) 24.0%-30.4%), higher among non-lean (39.8%) than lean individuals (17.4%). Lean individuals with OV infection had a statistically significant association with lower odds of SLD (adjusted odds ratio (aOR) 0.49, 95% CI 0.33-0.73). T2DM showed a significant positive association with SLD in both lean (aOR 3.58, 95% CI 2.28-5.63) and non-lean individuals (aOR 3.31, 95% CI 2.31-4.74) while dyslipidemia was significantly associated only in the non-lean group (aOR 1.83, 95% CI 1.09-3.07). Females participants exhibited elevated odds of SLD in both lean (aOR 1.43, 95% CI 1.02-2.01) and non-lean SLD (aOR 1.50, 95% CI 1.12-2.01). Conclusion: SLD prevalence is notably high among Laotian adults in rural areas, particularly in females and in non-lean individuals. Lean individuals with OV infection exhibited lower SLD prevalence. SLD was more prevalent in individuals with T2DM, independent of BMI. SLD adds to the burden of infection-related liver morbidity in Lao PDR. [ABSTRACT FROM AUTHOR]

Introduction: Cholangiocarcinoma (CCA) is a major contributor to hepatobiliary mortality in the Lao People's Democratic Republic (Lao PDR). Infection with the carcinogenic trematode Opisthorchis viverrini (OV), acquired through consumption of insufficiently-cooked river fish, is a known risk factor for the development of CCA. Together with OV, other risk factors contribute to the pathogenesis of CCA. We conducted this study to identify the burden of CCA and identify risk factors in high-risk communities in Lao PDR. Method: A cross-sectional study was performed in Champasack and Savannakhet provinces, southern Lao PDR, where OV infection is highly endemic. We assessed hepatobiliary morbidity with abdominal ultrasound (US). In addition, multiple risk factors known or suspected to be associated with CCA were assessed such as OV infection (examined by Kato-Katz technique for stool examination), lifestyle risks (e.g. smoking and alcohol consumption by face-to-face questionnaire), co-morbidity (e.g. diabetes mellitus) and hepatitis B infection status, both serologically tested. Results: In 3,400 participants, the overall prevalence of suspected CCA was 7.2% (95% confidence interval [95% CI] 5.4−9.6). The suspected CCA prevalence increased with age, and was higher in men at all ages. Almost all participants (88.3%) were infected with OV. In the multivariate regression analysis, suspected CCA was positively associated with OV infection (adjusted odds ratio [aOR] 3.4, 95% CI 1.7−6.5), and a history of cholecystectomy (aOR 2.7, 95% CI 1.5−4.9). Conclusion: Our CCA screening in high OV prevalence rural areas of Lao PDR uncovers a high public health burden, primarily driven by elevated OV infection rates. Urgent interventions are needed to curb OV infection in these communities. Age and gender disparities in suspected CCA prevalence highlight the need for targeted efforts. Beyond OV, notable factors like a history of cholecystectomy offer valuable insights for preventive strategies. This research enhances our understanding of hepatobiliary morbidity and informs public health initiatives in Lao PDR. Author summary: Evidence has long pointed to a significant burden of liver diseases in certain regions of the Lao People's Democratic Republic (Lao PDR), primarily attributed to the Opisthorchis viverrini (OV) liver fluke. This parasite is transmitted to humans through the consumption of raw or undercooked fish and fish products. Chronic OV infection can lead to cholangiocarcinoma (CCA), a fatal bile duct cancer. The objective of this research was to assess the prevalence of suspected CCA in rural OV-endemic communities in the southern part of Lao PDR. We conducted a cross-sectional study in selected communities within Savannakhet and Champasack provinces. Adults aged 35 years and older were enrolled and underwent liver disease assessment through abdominal ultrasound. OV infection was diagnosed via fecal examination, while lifestyle risk factors like smoking and alcohol consumption were evaluated through face-to-face interviews. Additionally, diabetes mellitus and hepatitis B infection status were determined serologically. We found a high prevalence of suspected bile duct cancer (CCA) at 7.2% overall. This rate was found higher in men compared to women, and to increase with age. Most participants (88.3%) were infected with OV, posing the highest risk for suspected CCA (adjusted odds ratio [aOR] 3.4). Additionally, a history of cholecystectomy (aOR 2.7) was a significant predictor of bile duct cancer. These results provide crucial insights for controlling liver morbidity in Lao PDR, emphasizing the urgent need to address OV infection and associated risk factors for CCA in affected communities. [ABSTRACT FROM AUTHOR]