25 results on '"Von Pinho, R. G."'
Search Results
2. Olfactory response of Trichogramma pretiosum (Hymenoptera: Trichogrammatidae) to volatiles induced by transgenic maize
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Nascimento, P. T., primary, Fadini, M. A. M., additional, Rocha, M. S., additional, Souza, C. S. F., additional, Barros, B. A., additional, Melo, J. O. F., additional, Von Pinho, R. G., additional, and Valicente, F. H., additional
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- 2021
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3. Influence of transgenic maize on behaviour of adult female of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: noctuidae)
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NASCIMENTO, P. T., VON PINHO, R. G., SOUZA, C. da S. F., FADINI, M. A. M., VALICENTE, F. H., Priscilla Tavares Nascimento, Universidade Federal de Lavras, Renzo Garcia Von Pinho, Camila da Silva Fernandes Souza, Universidade Federal de Lavras, Marcos Antonio Matiello Fadini, Universidade Federal de São João del-Rei, and FERNANDO HERCOS VALICENTE, CNPMS.
- Subjects
Milho transgênico ,Interação planta-herbívoro ,Lagarta-militar ,Transgenia ,Zea Mays ,Lagarta - Abstract
A manipulação genética de plantas pode causar efeitos pleiotróficos ou mutações oriundas da incorporação de um gene exógeno, os quais podem interferir no forrageamento de herbívoros. Pouco se conhece sobre os efeitos de plantas de milho tolerantes a herbicidas (evento singular), plantas resistentes a insetos (evento singular) e plantas com tolerância a herbicidas e resistência a insetos (evento estaqueado) sobre o forrageamento de Spodoptera frugiperda. O objetivo deste estudo foi avaliar se eventos de milho, singular e estaqueado, interferem na quantidade e qualidade dos ovos de S. frugiperda. Foram avaliados os híbridos DKB390 (VTPRO, VTPRO2, VTPRO3), DKB390 (isogênico) e Ag 3700 RR2. Testes de preferência de oviposição, com e sem chance de escolha, em plantas com e sem injúrias, por S. frugiperda foram realizados em casa de vegetação. Avaliouse o tamanho da massa de ovos e o número de lagartas eclodidas. Em plantas transgênicas, foram observadas massas de ovos de maior tamanho e maior número de lagartas eclodidas por planta. Conclui-se que fêmeas de S. frugiperda modificam o comportamento de oviposição diante de híbridos de milho transgênico e não transgênico, principalmente quando os híbridos apresentam injúrias. No entanto, não discriminaram entre híbrido singular ou estaqueado para oviposição. Made available in DSpace on 2020-11-12T19:29:03Z (GMT). No. of bitstreams: 1 Influence-transgenic.pdf: 619470 bytes, checksum: f3502d2f9418733129509a940c2b3cc0 (MD5) Previous issue date: 2020
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- 2020
4. Grain yield, anthesis-silking interval and drought tolerance indices of tropical maize hybrids
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SANTOS, A. de O., VON PINHO, R. G., SOUZA, V. F. de, GUIMARAES, L. J. M., BALESTRE, M., PIRES, L. P. M., SILVA, C. P. da, Álvaro de Oliveira Santos, Universidade Federal de Lavras, Renzo Garcia Von Pinho, Universidade Federal de Lavras, Vander Fillipe de Souza, Universidade Federal de Lavras, LAURO JOSE MOREIRA GUIMARAES, CNPMS, Márcio Balestre, Universidade Federal de Lavras, Luiz Paulo Miranda Pires, Universidade Federal de Lavras, and Carlos Pereira da Silva, Universidade Federal de Lavras.
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Milho ,Grão ,Rendimento ,Déficit hídrico ,Estresse hídrico ,Deficiência Hídrica ,Tolerância ao estresse - Abstract
Water deficit stress is the abiotic factor with the highest impact on crop yield. The objective of this study was to evaluate grain yield (GY), the anthesis-silking interval (ASI) and drought tolerance indices in maize hybrids. We evaluated GY and the ASI of 86 hybrids under two moisture levels (normal irrigation and water stress) for three consecutive years. The stress susceptibility index, water stress tolerance, drought resistance coefficient, drought resistance index, stress tolerance index and harmonic mean were evaluated. There were significant hybrid x environment interactions for GY and the ASI. Differences in the ASI among environments ranged from 0 to 5 days. The hybrids P3862, 1I873, 1I923, 1I862 and 1J1211 showed high GY, associated with the highest drought tolerance indices. The stress tolerance index and harmonic mean indices can be used to identify higher-yielding maize hybrids in environments with and without water restriction Made available in DSpace on 2020-04-15T01:01:16Z (GMT). No. of bitstreams: 1 Grainyield.pdf: 1830209 bytes, checksum: bb94f3b3d9bf3093fc7fd3bfc31b94af (MD5) Previous issue date: 2020
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- 2020
5. Identification of Double-Haploid Maize Plants One Generation After the Chromosomes Doubling
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Pires, R. M. O., primary, Ribeiro, B. G., additional, Bustamante, F. O., additional, Von Pinho, E. V. R., additional, Santos, H. O., additional, and Von Pinho, R. G., additional
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- 2019
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6. Does Singular and Stacked Corn Affect Choice Behavior for Oviposition and Feed in Spodoptera frugiperda(Lepidoptera: Noctuidae)?
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Nascimento, P. T., Von Pinho, R. G., Fadini, M. A. M., Souza, C. S. F., and Valicente, F. H.
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Little is known about the effects of genetically modified corn plants on the foraging of Spodoptera frugiperda(J.E. Smith). Therefore, this study examines whether singular herbicide-tolerant and insect-resistant plants and their stacked events interfere with food preference and oviposition of S. frugiperda. Two non-Bt corn hybrids and three Bt-hybrids, some of them with glyphosate tolerance (GT), were evaluated. Food preference of larvae and biological parameters were assessed. Oviposition preference bioassays involved choice and no choice condition in plants uninfested and previously infested by larvae in a greenhouse and in the field. The results indicate that there is no relationship between preference of larvae and adult moths. Adult females selected preferentially transgenic hybrids, while larvae selected non-Bt hybrid. Fall armyworm larvae avoid Bt-toxin-expressing leaf tissues, survived only on the non-Bt leaf tissues, and showed minor differences in other life-history traits reared on GT and non-transgenic corn leaf tissues. Female moths showed preference for transgenic plants to lay eggs, but with variable output between previously infested and uninfested plants with larvae. The fact that moths preferred Ag 3700RR2 and non-Bt hybrids for oviposition supports the refuge’s strategy aiming at producing susceptible individuals. The use of this hybrid must be integrated with a program of control. The results showed also the importance of correct hybrid selection as part of insect resistance management to Bt-plants. The implications of these findings for understanding the impacts of plant-mediated cues on pest behavior in transgenic crop systems are discussed.
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- 2020
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7. Maize hybrid stability in environments under water restriction using mixed models and factor analysis.
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Santos AO, Nuvunga JJ, Silva CP, Pires LPM, Von Pinho RG, Guimarães LJM, and Balestre M
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- Droughts, Environment, Models, Genetic, Quantitative Trait, Heritable, Zea mays growth & development, Genomic Instability, Hybridization, Genetic, Plant Breeding methods, Stress, Physiological, Zea mays genetics
- Abstract
In several crops, the water deficit is perhaps the main limiting factor in the search for high yields. The objective of this study was to evaluate the phenotypic stability of maize hybrids in environments with and without water restriction using the analytical factor (AF) approach. We evaluated 171 maize hybrids in 14 environments, divided into environments with (A1, A2, A3, A4, A5, A6, and A7) and without (A8, A9, A10, A11, A12, A13, and A14) water restriction, over a period of 7 years. Each year, 36 hybrids were evaluated. A square lattice design (6 x 6) was used, with common treatments between years. The characteristics of grain yield (GY), male flowering (MF) and female flowering (FF), plant height (PH), and ear height (EH) were evaluated. Phenotypic adaptability and stability of the hybrids were also verified. Hybrids G66, G99, G86, and G26 were the most stable and showed potential for use in environments with and without water restriction. The AF models showed to be useful for evaluating hybrids over many years, allowing selection of better hybrids with adaptability, specific and general stability, and correlation of hybrids with their production components, in addition to allowing identification of mega-environments that permit stability in the response of the adapted hybrids.
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- 2017
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8. Heat-resistant protein expression during germination of maize seeds under water stress.
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Abreu VM, Silva Neta IC, Von Pinho EV, Naves GM, Guimarães RM, Santos HO, and Von Pinho RG
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- Dehydration genetics, Dehydration metabolism, Gene Expression, Gene Expression Regulation, Plant, Heat-Shock Proteins metabolism, Heat-Shock Response, Hot Temperature, Plant Proteins metabolism, Seeds genetics, Seeds growth & development, Zea mays genetics, Zea mays growth & development, Germination, Heat-Shock Proteins genetics, Plant Proteins genetics, Seeds metabolism, Zea mays metabolism
- Abstract
Low water availability is one of the factors that limit agricultural crop development, and hence the development of genotypes with increased water stress tolerance is a challenge in plant breeding programs. Heat-resistant proteins have been widely studied, and are reported to participate in various developmental processes and to accumulate in response to stress. This study aimed to evaluate heat-resistant protein expression under water stress conditions during the germination of maize seed inbreed lines differing in their water stress tolerance. Maize seed lines 91 and 64 were soaked in 0, -0.3, -0.6, and -0.9 MPa water potential for 0, 6, 12, 18, and 24 h. Line 91 is considered more water stress-tolerant than line 64. The analysis of heat-resistant protein expression was made by gel electrophoresis and spectrophotometry. In general, higher expression of heat-resistant proteins was observed in seeds from line 64 subjected to shorter soaking periods and lower water potentials. However, in the water stress-tolerant line 91, a higher expression was observed in seeds that were subjected to -0.3 and -0.6 MPa water potentials. In the absence of water stress, heat-resistant protein expression was reduced with increasing soaking period. Thus, there was a difference in heat-resistant protein expression among the seed lines differing in water stress tolerance. Increased heat-resistant protein expression was observed in seeds from line 91 when subjected to water stress conditions for longer soaking periods., Competing Interests: The authors declare no conflict of interest.
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- 2016
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9. Genomic breeding value prediction for simple maize hybrid yield using total effects of associated markers, under different imbalance levels and environments.
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Cantelmo NF, Von Pinho RG, and Balestre M
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- Brazil, Environment, Genetic Markers genetics, Genome, Plant, Models, Genetic, Phenotype, Quantitative Trait Loci, Crosses, Genetic, Microsatellite Repeats, Plant Breeding methods, Zea mays genetics
- Abstract
The main objective of a maize breeding program is to generate hybrid combinations that are more productive than those pre-existing in the market. However, the number of parents, and consequently the number of crosses, increases so rapidly that the phenotypic evaluation of all the possible combinations becomes economically and technically infeasible. In this context, predicting the performance of the most promising genotypes may increase the genetic gains with increased selection intensity and reduced breeding cycles. Thus, the present study aimed to use the total effects of associated markers method to predict genomic breeding values (GBVs) via cross-validation and by using different imbalance levels (10, 30, 50, and 70%). A set of 51 genotyped strains was used with 79 microsatellite markers and 273 hybrids that were generated by a partial diallel. A total of 186 and 272 hybrids were analyzed in the experiments within the southern and central regions of Brazil, respectively. The GBVs were, thus, predicted for each location in both the regions, and for training in one region and validation in another region. The correlation between the predicted and observed GBVs ranged from 0.48 to 0.91, depending on the imbalance level and the region analyzed. Overall, the results obtained in the present study were promising, particularly considering that a small number of markers were used and that the training and predictions occurred in the very distinct regions of southern and central Brazil.
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- 2016
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10. Genome-wide prediction of maize single-cross performance, considering non-additive genetic effects.
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Santos JP, Pereira HD, Von Pinho RG, Pires LP, Camargos RB, and Balestre M
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- Algorithms, Bayes Theorem, Breeding, Chimera, Databases, Genetic, Epistasis, Genetic, Models, Genetic, Phenotype, Zea mays genetics, Crosses, Genetic, Genome, Plant, Genome-Wide Association Study
- Abstract
The prediction of single-cross hybrids in maize is a promising technique for optimizing the use of financial resources in a breeding program. This study aimed to evaluate Genomic Best Linear Unbiased Predictors models for hybrid prediction and compare them with the Bayesian Ridge Regression, Bayes A, Bayesian LASSO, Bayes C, Bayes B, and Reproducing Kernel Hilbert Spaces Regression models, with inclusion or absence of non-additive effects under three heritability scenarios. Data from a maize germplasm bank belonging to USDA were used to determine the effects of molecular markers, which were considered to be parametric, to build 400 single-cross hybrids between two line groups via simulation. The following parameters were used to compare the models: predictive ability, estimation of variance components, heritability of genetic effects present in all situations, and the sum of squares of the predicted errors. The models responded positively when dominance effects were included in non-additive models, with all models tending to show an increase in the values of heritability parameters under all scenarios. Differences occur between models depending on the heritability range considered. Estimates of additive and dominant effects were better than estimates of epistatic effects. Estimates increased in accuracy for all models when non-additive effects for maize cob weight were considered.
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- 2015
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11. Genes related to high temperature tolerance during maize seed germination.
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Dutra SM, Von Pinho EV, Santos HO, Lima AC, Von Pinho RG, and Carvalho ML
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- Gene Expression Regulation, Plant, Plant Proteins biosynthesis, Plant Proteins genetics, Seeds physiology, Temperature, Zea mays physiology, alpha-Amylases biosynthesis, Germination genetics, Seeds genetics, Stress, Physiological genetics, Zea mays genetics
- Abstract
The identification of genes related to heat tolerance is fundamental for the development of high-quality seeds that are tolerant to heat stress condition. The objective of this study was to evaluate maize lineages and the gene expression involved in high temperature tolerance during germination using physiological tests, proteomics, and transcriptome analysis. Seeds from six maize lineages (30, 44, 54, 63, 64, and 91) with different levels of tolerance to high temperatures were used. Lineages 54 and 91 were observed to be more tolerant to high temperature conditions. The highest expression of α-amylase was observed in maize seeds from lineages 30 and 91 that were subjected to controlled deterioration. The highest expression of α-amylase was observed in maize seeds from lineages 30 and 91 that were subjected to controlled deterioration; with the controlled deterioration, the highest level of gene expression did not occur in the most tolerant materials; the association of lower expression of genes involved in heat-resistant protein systems was observed in seeds from lineage 44, which were more susceptible to high temperatures, and the highest gene expression of LEA D-34, ZmAN13, and AOX-1 was observed in seeds from lineage 64 when submitted to controlled deterioration.
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- 2015
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12. Indirect selection for resistance to ear rot and leaf diseases in maize lines using biplots.
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Pereira GS, Camargos RB, Balestre M, Von Pinho RG, and C Melo WM
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- Ascomycota physiology, Disease Resistance, Edible Grain genetics, Edible Grain growth & development, Edible Grain microbiology, Fusarium physiology, Genes, Plant, Genetic Predisposition to Disease, Plant Diseases genetics, Plant Leaves genetics, Plant Leaves growth & development, Plant Leaves microbiology, Selection, Genetic, Zea mays genetics, Zea mays growth & development, Plant Diseases microbiology, Zea mays microbiology
- Abstract
Leaf disease and ear rot have caused reductions in maize yield in Brazil and other producer countries. Therefore, the aims of this study were to analyze the association between husked ear yield and the severity of maize white spot, gray leaf spot, helminthosporium, and ear rot caused by Fusarium verticillioides and Diplodia maydis using biplots in a mixed-model approach. The responses of 238 lines introduced to Brazil and four controls were evaluated using an incomplete block design with three replicates in two locations: Lavras and Uberlândia, Minas Gerais, Brazil. Two experiments were conducted in each location, one with F. verticillioides and the other with D. maydis. The mixed models elucidated the relationship between yield, leaf disease, and ear disease. Significant genotype x environment and genotype x pathogen interactions were observed. In conclusion, husked ear yield is more associated with ear rot than with the leaf diseases evaluated, justifying the indirect selection for resistance to kernel rot in maize-F. verticillioides and maize-D. maydis pathosystems by yield evaluation.
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- 2015
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13. Verification and characterization of chromosome duplication in haploid maize.
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de Oliveira Couto EG, Resende Von Pinho EV, Von Pinho RG, Veiga AD, de Carvalho MR, de Oliveira Bustamante F, and Nascimento MS
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- Cell Survival, Chimera, Crosses, Genetic, DNA, Plant analysis, Flow Cytometry, Homozygote, Microsatellite Repeats, Ploidies, Pollen growth & development, Pollen ultrastructure, Seeds growth & development, Seeds ultrastructure, Staining and Labeling, Zea mays growth & development, Zea mays ultrastructure, Chromosome Duplication, Chromosomes, Plant, DNA, Plant genetics, Pollen genetics, Seeds genetics, Zea mays genetics
- Abstract
Doubled haploid technology has been used by various private companies. However, information regarding chromosome duplication methodologies, particularly those concerning techniques used to identify duplication in cells, is limited. Thus, we analyzed and characterized artificially doubled haploids using microsatellites molecular markers, pollen viability, and flow cytometry techniques. Evaluated material was obtained using two different chromosome duplication protocols in maize seeds considered haploids, resulting from the cross between the haploid inducer line KEMS and 4 hybrids (GNS 3225, GNS 3032, GNS 3264, and DKB 393). Fourteen days after duplication, plant samples were collected and assessed by flow cytometry. Further, the plants were transplanted to a field, and samples were collected for DNA analyses using microsatellite markers. The tassels were collected during anthesis for pollen viability analyses. Haploid, diploid, and mixoploid individuals were detected using flow cytometry, demonstrating that this technique was efficient for identifying doubled haploids. The microsatellites markers were also efficient for confirming the ploidies preselected by flow cytometry and for identifying homozygous individuals. Pollen viability showed a significant difference between the evaluated ploidies when the Alexander and propionic-carmin stains were used. The viability rates between the plodies analyzed show potential for fertilization.
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- 2015
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14. Production and identification of doubled haploids in tropical maize.
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Battistelli GM, Von Pinho RG, Justus A, Couto EG, and Balestre M
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- Chromosome Duplication, Chromosomes, Plant genetics, Genes, Plant, Genetic Markers, Hybridization, Genetic, Microsatellite Repeats, Tropical Climate, Haploidy, Zea mays genetics
- Abstract
The production of maize doubled haploid (DH) lines is a technique commonly used by private companies, but not by Brazilian public institutions. Research on this technique is essential to develop and improve the production of DH lines grown under tropical conditions. We assessed the ability of a gynogenetic haploid inducer system to induce haploids in a tropical environment, assessed the induction rate of haploids identified using the R-navajo morphological marker, checked for interference from the generation of hybrid donors on haploid induction, measured the ability of flow cytometry, and simple sequence repeat marker techniques to identify doubled haploids. Seeds from the inducer Krasnodar Embryo Marker Synthetic (KEMS) line were sown in Ponta Grossa, PR, and Cravinhos, SP, and the plants were crossed to produce six hybrids and their F2 generations. The seeds were separated according to the R-navajo morphological marker indicator of haploidy (purple endosperm and white embryo) and germinated in a controlled environment. Chromosomal duplication was performed in seedlings selected as putative haploids. We performed subsequent confirmation of ploidy and the success of duplication using flow cytometry and SSR marker techniques. We concluded that DH lines can be obtained from hybrids crossed with the inducer KEMS line. The generation of inbred hybrids did not affect the induction rate or chromosomal duplication in haploids. The use of flow cytometry and SSR markers was effective in verifying chromosomal duplication in haploids.
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- 2013
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15. Physiological quality and gene expression related to heat-resistant proteins at different stages of development of maize seeds.
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Andrade T, Von Pinho EV, Von Pinho RG, Oliveira GE, Andrade V, and Fernandes JS
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- DNA, Plant genetics, Electrophoresis, Germination genetics, Plant Proteins metabolism, Real-Time Polymerase Chain Reaction, Seeds physiology, Sequence Analysis, DNA, Spectrophotometry, Zea mays physiology, Gene Expression Regulation, Plant, Hot Temperature, Plant Proteins genetics, Seeds genetics, Zea mays genetics
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We quantified and characterized the expression of heat-resistant proteins during seed development of maize lines with distinct levels of tolerance to high drying temperature. A corn field was planted for multiplication of seeds of different lines, two tolerant and two non-tolerant to high drying temperatures. Harvest of the seeds was carried out at various stages of development and they were then subjected to tests of moisture content, germination, first count of germination, accelerated aging, and cold test. The seeds were stored in a freezer for later analysis of expression of heat-resistant proteins by means of real-time PCR, electrophoresis, and spectrophotometry. We observed that heat-resistant proteins are expressed in a differential manner in seeds from different lines and at different stages of development. The expression of heat-resistant proteins was earlier in lines tolerant to high drying temperatures. Greater germination and vigor values was found for seeds collected at the last stage of development.
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- 2013
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16. Detection limits of the strip test and PCR for genetically modified corn in Brazil.
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Nascimento VE, Von Pinho ÉV, Von Pinho RG, and do Nascimento AD Jr
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- Brazil, Genes, Plant genetics, Plants, Genetically Modified, Zein genetics, Limit of Detection, Polymerase Chain Reaction methods, Zea mays genetics
- Abstract
Brazilian legislation establishes a labeling limit for products that contain more than 1% material from genetically modified organisms (GMOs). We assessed the sensitivity of the lateral flow strip test in detection of the GMO corn varieties Bt11 and MON810 and the specificity and sensitivity of PCR techniques for their detection. For the strip test, the GMO seeds were mixed with conventional seeds at levels of 0.2, 0.4 and 0.8% for Bt11, and 0.4, 0.8 and 1.6% for MON810. Three different methodologies were assessed and whole seeds, their endosperm and embryonic axis were used. For the PCR technique, the GMO seeds of each of the two varieties were mixed with conventional seeds at levels of 20, 10, 5, 2, 1, and 0.5%. The seeds were ground and the DNA extracted. For detection of the GMO material, specific primers were used for MON810 and Bt11 and maize zein as an endogenous control. The sensitivity of the strip test varied for both maize varieties and methodologies. The test was positive for Bt11 only at 0.8%, in contrast with the detection limit of 0.4% indicated by the manufacturer. In the multiplex PCR, the primers proved to be specific for the different varieties. These varieties were detected in samples with one GMO seed in 100. Thus, this technique proved to be efficient in detecting contaminations equal to or greater than 1%.
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- 2012
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17. Bayesian inference to study genetic control of resistance to gray leaf spot in maize.
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Balestre M, Von Pinho RG, and Brito AH
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- Ascomycota pathogenicity, Bayes Theorem, Mycoses pathology, Plant Leaves microbiology, Zea mays microbiology, Disease Resistance genetics, Plant Diseases genetics, Plant Leaves genetics, Zea mays genetics
- Abstract
Gray leaf spot (GLS) is a major maize disease in Brazil that significantly affects grain production. We used Bayesian inference to investigate the nature and magnitude of gene effects related to GLS resistance by evaluation of contrasting lines and segregating populations. The experiment was arranged in a randomized block design with three replications and the mean values were analyzed using a Bayesian shrinkage approach. Additive-dominant and epistatic effects and their variances were adjusted in an over-parametrized model. Bayesian shrinkage analysis showed to be an excellent approach to handle complex models in the study of genetic control in GLS, since this approach allows to handle overparametrized models (main and epistatic effects) without using model-selection methods. Genetic control of GLS resistance was predominantly additive, with insignificant influence of dominance and epistasis effects.
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- 2012
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18. Prediction of maize double-cross hybrids using the best linear unbiased prediction with microsatellite marker information.
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Balestre M, Von Pinho RG, and Souza JC
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- Genes, Plant, Genotype, Models, Genetic, Chimera genetics, Crosses, Genetic, Microsatellite Repeats genetics, Phenotype, Zea mays genetics
- Abstract
We examined the usefulness of the best linear unbiased prediction associated with molecular markers for prediction of untested maize double-cross hybrids. Ten single-cross hybrids from different commercial backgrounds were crossed using a complete diallel design. These 10 single-cross hybrids were genotyped with 20 microsatellite markers. The best linear unbiased prediction associated with microsatellite information gave relatively good prediction ability of the double-cross hybrid performance, with correlations between observed phenotypic values and genotypic prediction values varying from 0.27 to 0.54. Taking into account the predictions of specific combing ability, the correlation between observed and predicted specific combining ability varied from 0.50 to 0.88. Based on these results, we infer that it is feasible to predict maize double-cross hybrids with different unbalance degrees without including any prior information about parental inbreed lines or single-cross hybrid performance.
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- 2011
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19. Prediction of maize hybrid performance using similarity in state and similarity by descent information.
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Ferreira DV, Von Pinho RG, Balestre M, and Oliveira RL
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- Microsatellite Repeats genetics, Quantitative Trait Loci, Zea mays genetics
- Abstract
We evaluated the efficiency of the best linear unbiased predictor (BLUP) and the influence of the use of similarity in state (SIS) and similarity by descent (SBD) in the prediction of untested maize hybrids. Nine inbred lines of maize were crossed using a randomized complete diallel method. These materials were genotyped with 48 microsatellite markers (SSR) associated with the QTL regions for grain yield. Estimates of four coefficients of SIS and four coefficients of SBD were used to construct the additive genetic and dominance matrices, which were later used in combination with the BLUP for predicting genotypic values and specific combining ability (SCA) in unanalyzed hybrids under simulated unbalance. The values of correlations between the genotypic values predicted and the means observed, depending on the degree of unbalance, ranged from 0.48 to 0.99 for SIS and 0.40 to 0.99 using information from SBD. The results obtained for the SCA ranged from 0.26 to 0.98 using the SIS and 0.001 to 0.990 using the SBD information. It was also observed that the predictions using SBD showed less biased than SIS predictions demonstrating that the predictions obtained by these coefficients (SBD) were closer to the observed value, but were less efficient in the ranking of genotypes. Although the SIS showed a bias due to overestimation of relatedness, this type of coefficient may be used where low values are detected in the SBD in the group of parents because of its greater efficiency in ranking the candidates hybrids.
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- 2010
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20. Prediction of maize single-cross performance by mixed linear models with microsatellite marker information.
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Balestre M, Von Pinho RG, and Souza JC
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- Brazil, Crosses, Genetic, Genes, Plant, Genotype, Linear Models, Models, Genetic, Models, Statistical, Phenotype, Microsatellite Repeats genetics, Quantitative Trait Loci, Zea mays genetics
- Abstract
We evaluated the potential of the best linear unbiased predictor (BLUP) along with the relationship coefficient for predicting the performance of untested maize single-cross hybrids. Ninety S(0:2) progenies arising from three single-cross hybrids were used. The 90 progenies were genotyped with 25 microsatellite markers, with nine markers linked to quantitative trait loci for grain yield. Based on genetic similarities, 17 partial inbred lines were selected and crossed in a partial diallel design. Similarity and relationship coefficients were used to construct the additive and dominance genetic matrices; along with BLUP, they provided predictions for untested single-crosses. Five degrees of imbalance were simulated (5, 10, 20, 30, and 40 hybrids). The correlation values between the predicted genotypic values and the observed phenotypic means varied from 0.55 to 0.70, depending on the degree of imbalance. A similar result was observed for the specific combining ability predictions; they varied from 0.61 to 0.70. It was also found that the relationship coefficient based on BLUP provided more accurate predictions than similarity-in-state predictions. We conclude that BLUP methodology is a viable alternative for the prediction of untested crosses in early progenies.
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- 2010
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21. Genotypic stability and adaptability in tropical maize based on AMMI and GGE biplot analysis.
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Balestre M, Von Pinho RG, Souza JC, and Oliveira RL
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- Crosses, Genetic, Models, Genetic, Zea mays physiology, Adaptation, Physiological, Zea mays genetics
- Abstract
We evaluated the phenotypic and genotypic stability and adaptability of hybrids using the additive main effect and multiplicative interaction (AMMI) and genotype x genotype-environment interaction (GGE) biplot models. Starting with 10 single-cross hybrids, a complete diallel was done, resulting in 45 double-cross hybrids that were appraised in 15 locations in Southeast, Center-West and Northeast Brazil. In most cases, when the effects were considered as random (only G effects or G and GE simultaneously) in AMMI and GGE analysis, the distances between predicted values and observed values were smaller than for AMMI and GGE biplot phenotypic means; the best linear unbiased predictors of G and GE generally showed more accurate predictions in AMMI and GGE analysis. We found the GGE biplot method to be superior to the AMMI 1 graph, due to more retention of GE and G + GE in the graph analysis. However, based on cross-validation results, the GGE biplot was less accurate than the AMMI 1 graph, inferring that the quantity of GE or G + GE retained in the graph analysis alone is not a good parameter for choice of stabilities and adaptabilities when comparing AMMI and GGE analyses.
- Published
- 2009
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22. Potential use of molecular markers for prediction of genotypic values in hybrid maize performance.
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Balestre M, Von Pinho RG, Souza JC, and Oliveira RL
- Subjects
- Genotype, Hybridization, Genetic, Models, Genetic, Genetic Markers, Zea mays genetics
- Abstract
We evaluated the potential of genetic distances estimated by microsatellite markers for the prediction of the performance of single-cross maize hybrids. We also examined the potential of molecular markers for the prediction of genotypic values and the applicability of the Monte Carlo method for a correlation of genetic distances and grain yield. Ninety S(0:2) progenies derived from three single-cross hybrids were analyzed. All 90 progenies were genotyped with 25 microsatellite markers, including nine markers linked to quantitative trait loci for grain yield. The genetic similarity datasets were used for constructing additive genetic and dominance matrices that were subsequently used to obtain the best linear unbiased prediction of specific combining ability and general combining ability. The genetic similarities were also correlated with grain yield, specific combining ability and heterosis of the hybrids. Genetic distances had moderate predictive ability for grain yield (0.546), specific combining ability (0.567) and heterosis (0.661). The Monte Carlo simulation was found to be a viable alternative for a correlation of genetic distances and grain yield. The accuracy of genotypic values using molecular data information was slightly higher than if no such information was incorporated. The estimation of the relationship using molecular markers proved to be a promising method for predicting genetic values using mixed linear models, especially when information about pedigree is unavailable.
- Published
- 2009
- Full Text
- View/download PDF
23. Potential of maize single-cross hybrids for extraction of inbred lines using the mean components and mixed models with microsatellite marker information.
- Author
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Balestre M, Von Pinho RG, Souza JC, and Machado JC
- Subjects
- Crosses, Genetic, Models, Genetic, Chimera genetics, Hybridization, Genetic genetics, Microsatellite Repeats genetics, Zea mays genetics
- Abstract
The present study examined the importance of mean (m+a' and d) components in the performance of single-cross hybrids for the formation of new populations and determined the contribution of the mixed model (best linear unbiased predictor of random effects, BLUP) method associated with molecular markers for the choice of crosses to obtain interpopulation hybrids. Ten single-cross commercial hybrids of different companies were used for this purpose, producing all possible double-cross hybrids through a complete diallel. The hybrids were evaluated in 15 locations in the agricultural year 2005/2006, using randomized complete block design with three repetitions. In three of these locations, estimates of m+a' and d were obtained. DNA was extracted from the single-cross hybrids and 20 SSR primers were used, nine of which were linked to QTL for yield. There was no correlation between m+a' of the single-cross hybrids with general combining ability (r = -0.15) inferring that populations with lines with high means do not always produce good hybrids. Also, it was observed that the correlation between the genetic distances with specific combining ability varied from 0.31 to 0.80 in the inter-group hybrids, while in the intra-group hybrids these estimates were low and non-significant. The heritability value obtained by BLUP was high and greater than that obtained by ordinary least squares (h(2) = 0.95 and 0.86), confirming the greater selection accuracy by the BLUP method. There were no differences between the accuracy values obtained with microsatellite information and without this information, inferring that there was no advantage of progenitor information on balanced data. It can be concluded that the estimate m+a' should not be used as a deciding parameter about the potential for extracting lines from a given population. The heritability and accuracy values obtained by BLUP allow the inference that it is possible to predict success in the choice of progenitors to obtain interpopulation hybrids.
- Published
- 2008
- Full Text
- View/download PDF
24. Comparison of maize similarity and dissimilarity genetic coefficients based on microsatellite markers.
- Author
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Balestre M, Von Pinho RG, Souza JC, and Lima JL
- Subjects
- DNA, Plant genetics, Genetic Variation, Microsatellite Repeats, Zea mays genetics
- Abstract
The present study compared different similarity and dissimilarity coefficients and their influence in maize inbred line clustering. Ninety maize S0:1 inbred lines were used and genotyped with 25 microsatellite markers (simple sequence repeat). The simple matching, Rogers and Tanimoto, Russel and Rao, Hamann, Jaccard, Sorensen-Dice, Ochiai, and Roger's modified distance coefficients were compared by consensus index, projection efficiency in a two-dimensional space and by Spearman's correlation. Changes were found in high genetic similarity groupings with different coefficients using the consensus index. Russel and Rao and Jaccard coefficients had the greatest stress values with 75.67 and 40.16%, respectively, indicating that these coefficients should not be used. Genotype ranking changed, mainly in the comparison of the Roger's modified distance in relation to some coefficients (rs = 0.75). Russel and Rao's and Jaccard's coefficients should be avoided for their low accuracy. Moreover, genotype clustering by different similarly coefficients, without a close consideration of these coefficients could affect the research results.
- Published
- 2008
- Full Text
- View/download PDF
25. Mapping of quantitative trait locus associated with maize tolerance to high seed drying temperature.
- Author
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Salgado KC, Von Pinho EV, Guimarães CT, Von Pinho RG, Ferreira CA, and Andrade V
- Subjects
- Adaptation, Physiological, Chromosome Mapping, Chromosomes, Plant genetics, Genetic Variation, Phenotype, Quantitative Trait Loci genetics, Seeds genetics, Temperature, Zea mays genetics, Zea mays physiology
- Abstract
quantitative trait locus (QTL) mapping and identification of traits of agronomic importance is important in the process of molecular marker-assisted selection in breeding programs. The molecular map of maize is well saturated and QTL and simple sequence repeat (SSR) markers have been identified, whereas few markers linked to seed quality traits are included. The present study aimed to identify QTL and the gene action and to quantify the effects of these regions in the phenotypic variation related to maize tolerance to high seed drying temperature. SSR markers and 129 segregating families of F2 plants of the cross of intolerant and tolerant lines were used in regression and composite interval mapping methods. Three maize QTL associated with tolerance to high seed drying temperature were identified and mapped to chromosomes 6 and 8, explaining 39% of the phenotypic variation of the trait with additive, dominance and overdominance gene action. These markers seem to be effectively associated with the evaluated trait, since all were mapped near genes whose expression products were associated with seed desiccation tolerance.
- Published
- 2008
- Full Text
- View/download PDF
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