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1. Aspects of Campus Climate and Mental Health Threats: The Role of Hypervigilance

Author

Timmerman, Joanna R. and Volpe, V.

Abstract

Objective: This study sought to examine if hypervigilance is one mechanism through which aspects of less supportive campus climates are associated with mental health symptoms for college students. Participants: Data from 386 undergraduate college students attending a small college in the northeastern United States were collected. Methods: Participants completed online surveys which employed established measures of study variables. Results: Hypervigilance mediated the association between subjective social status and symptoms of anxiety and depression; lower subjective social status was associated with greater hypervigilance and greater hypervigilance was associated with more symptoms of anxiety and depression. Less sense of community was also directly associated with more anxiety, depression, and somatic symptoms. Conclusions: Hypervigilance may be an adaptive strategy to protect against psychosocial harm for low status members of the campus community, but may damage longer-term mental health. Implications for higher education administrators are discussed.

Published
2023
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2. Conceptual design of the AGATA 2[formula omitted] array at LNL

Author

Valiente-Dobón, J.J., Menegazzo, R., Goasduff, A., Agguiaro, D., Aguilera, P., Angelini, F., Balogh, M., Bazzacco, D., Benito, J., Benzoni, G., Bez, N., Bolognesi, M., Bottoni, S., Brugnara, D., Carollo, S., Cocconi, P., Cogo, A., Collado, J., Crespi, F.C.L., Ertoprak, A., Escudeiro, R., Galtarossa, F., Gamba, E.R., Gambalonga, A., Servín, B. Góngora, Gottardo, A., Gozzelino, A., Gulmini, M., Huang, Z., Marchi, T., Mengoni, D., Modanese, P., Napoli, D.R., Pellumaj, J., Pérez-Vidal, R.M., Pigliapoco, S., Pilotto, E., Ramina, L., Rampazzo, M., Raniero, W., Rebeschini, M., Rezynkina, K., Rosso, D., Scarcioffolo, M., Scarpa, D., Sedlák, M., Smith, R., Toniolo, N., Veronese, F., Volpe, V., Zago, L., Zanon, I., Zhang, G., Abels, R., Allegrini, M.L., Aufranc, C., Baulieu, G., Belkhiria, C., Benettoni, M., Benini, D., Bentley, M., Biasotto, M., Blaizot, M., Miquel, J. Blasco, Boiano, C., Boston, A., Boston, H., Boujrad, A., Bourgault, P., Bracco, A., Brambilla, S., Burrows, I., Camera, F., Capra, S., Capsoni, A., Cash, R., Civera, J.V., Clément, E., Coelli, S., Cordwell, M., Corradi, L., Coudert, S., De Angelis, G., De Ruvo, L., Debras, G., Del Fabbro, M., Diklić, J., Dosme, N., Duchene, G., Duclos, B., Dudouet, J., Eberth, J., Elloumi, S., Everett, C., Fantinel, S., Fillinger, M., Fioretto, E., Fransen, C., Gadea, A., Gibelin, L., González, V., Goupil, J., Görgen, C., Grant, A., Green, K., Ha, J., Hartnett, T., Henseler, K., Hess, H., Hirsch, R., Houarner, C., Jacob, J., Joannem, T., Judson, D.S., Karkour, N., Karolak, M., Kebbiri, M., Kieffer, J., Labiche, M., Lafay, X., Le Jeannic, P., Lefevre, A., Legay, E., Legruel, F., Lenzi, S., Leoni, S., Linget, D., Liptrot, M., López-Martens, A., Lotodé, A., Manara, L., Ménager, L., Mijatović, T., Million, B., Minarello, A., Montagnoli, G., Morrall, P., Mullacrane, I., Nyberg, J., Philippon, G., Polettini, M., Popieul, F., Pullia, A., Recchia, F., Reiter, P., Richardt, G., Rocchini, M., Roger, A., Saillant, F., Sanchis, E., Laskar, Md.S.R., Secci, G., Sigward, M.-H., Simpson, J., Solenne, N., Spee, F., Stefanini, A.M., Stézowski, O., Szilner, S., Templeton, N., Theisen, Ch., Thiel, S., Tomasi, F., Tzvetkov, S., Vigano, D., Viscione, E., Wieland, O., Wimmer, K., Wittwer, G., and Zielińska, M.

Published
2023
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3. The GALILEO [formula omitted]-ray array at the Legnaro National Laboratories

Author

Goasduff, A., Mengoni, D., Recchia, F., Valiente-Dobón, J.J., Menegazzo, R., Benzoni, G., Barrientos, D., Bellato, M., Bez, N., Biasotto, M., Blasi, N., Boiano, C., Boso, A., Bottoni, S., Bracco, A., Brambilla, S., Brugnara, D., Camera, F., Capra, S., Capsoni, A., Cocconi, P., Coelli, S., Cortés, M.L., Crespi, F.C.L., de Angelis, G., Egea, F.J., Fanin, C., Fantinel, S., Gadea, A., Gamba, E.R., Gambalonga, A., Gesmundo, C., Gosta, G., Gottardo, A., Gozzelino, A., Gregor, E.T., Gulmini, M., Ha, J., Hadyńska-Klȩk, K., Illana, A., Isocrate, R., Jaworski, G., John, P.R., Lenzi, S.M., Leoni, S., Lunardi, S., Magalini, M., Marchini, N., Million, B., Modamio, V., Nannini, A., Napoli, D.R., Pasqualato, G., Pellumaj, J., Pérez-Vidal, R.M., Pigliapoco, S., Polettini, M., Porzio, C., Pullia, A., Ramina, L., Rampazzo, G., Rampazzo, M., Rebeschini, M., Rezynkina, K., Rocchini, M., Romanato, M., Rosso, D., Saltarelli, A., Scarcioffolo, M., Siciliano, M., Testov, D.A., Tomasella, D., Tomasi, F., Toniolo, N., Ur, C.A., Ventura, S., Veronese, F., Viscione, E., Volpe, V., Wieland, O., Zanon, I., Ziliani, S., Zhang, G., and Bazzacco, D.

Published
2021
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5. Conceptual design of the AGATA 2π array at LNL

Author

Valiente-Dobón, J.J., primary, Menegazzo, R., additional, Goasduff, A., additional, Agguiaro, D., additional, Aguilera, P., additional, Angelini, F., additional, Balogh, M., additional, Bazzacco, D., additional, Benito, J., additional, Benzoni, G., additional, Bez, N., additional, Bolognesi, M., additional, Bottoni, S., additional, Brugnara, D., additional, Carollo, S., additional, Cocconi, P., additional, Cogo, A., additional, Collado, J., additional, Crespi, F.C.L., additional, Ertoprak, A., additional, Escudeiro, R., additional, Galtarossa, F., additional, Gamba, E.R., additional, Gambalonga, A., additional, Servín, B. Góngora, additional, Gottardo, A., additional, Gozzelino, A., additional, Gulmini, M., additional, Huang, Z., additional, Marchi, T., additional, Mengoni, D., additional, Modanese, P., additional, Napoli, D.R., additional, Pellumaj, J., additional, Pérez-Vidal, R.M., additional, Pigliapoco, S., additional, Pilotto, E., additional, Ramina, L., additional, Rampazzo, M., additional, Raniero, W., additional, Rebeschini, M., additional, Rezynkina, K., additional, Rosso, D., additional, Scarcioffolo, M., additional, Scarpa, D., additional, Sedlák, M., additional, Smith, R., additional, Toniolo, N., additional, Veronese, F., additional, Volpe, V., additional, Zago, L., additional, Zanon, I., additional, Zhang, G., additional, Abels, R., additional, Allegrini, M.L., additional, Aufranc, C., additional, Baulieu, G., additional, Belkhiria, C., additional, Benettoni, M., additional, Benini, D., additional, Bentley, M., additional, Biasotto, M., additional, Blaizot, M., additional, Miquel, J. Blasco, additional, Boiano, C., additional, Boston, A., additional, Boston, H., additional, Boujrad, A., additional, Bourgault, P., additional, Bracco, A., additional, Brambilla, S., additional, Burrows, I., additional, Camera, F., additional, Capra, S., additional, Capsoni, A., additional, Cash, R., additional, Civera, J.V., additional, Clément, E., additional, Coelli, S., additional, Cordwell, M., additional, Corradi, L., additional, Coudert, S., additional, De Angelis, G., additional, De Ruvo, L., additional, Debras, G., additional, Del Fabbro, M., additional, Diklić, J., additional, Dosme, N., additional, Duchene, G., additional, Duclos, B., additional, Dudouet, J., additional, Eberth, J., additional, Elloumi, S., additional, Everett, C., additional, Fantinel, S., additional, Fillinger, M., additional, Fioretto, E., additional, Fransen, C., additional, Gadea, A., additional, Gibelin, L., additional, González, V., additional, Goupil, J., additional, Görgen, C., additional, Grant, A., additional, Green, K., additional, Ha, J., additional, Hartnett, T., additional, Henseler, K., additional, Hess, H., additional, Hirsch, R., additional, Houarner, C., additional, Jacob, J., additional, Joannem, T., additional, Judson, D.S., additional, Karkour, N., additional, Karolak, M., additional, Kebbiri, M., additional, Kieffer, J., additional, Labiche, M., additional, Lafay, X., additional, Le Jeannic, P., additional, Lefevre, A., additional, Legay, E., additional, Legruel, F., additional, Lenzi, S., additional, Leoni, S., additional, Linget, D., additional, Liptrot, M., additional, López-Martens, A., additional, Lotodé, A., additional, Manara, L., additional, Ménager, L., additional, Mijatović, T., additional, Million, B., additional, Minarello, A., additional, Montagnoli, G., additional, Morrall, P., additional, Mullacrane, I., additional, Nyberg, J., additional, Philippon, G., additional, Polettini, M., additional, Popieul, F., additional, Pullia, A., additional, Recchia, F., additional, Reiter, P., additional, Richardt, G., additional, Rocchini, M., additional, Roger, A., additional, Saillant, F., additional, Sanchis, E., additional, Laskar, Md.S.R., additional, Secci, G., additional, Sigward, M.-H., additional, Simpson, J., additional, Solenne, N., additional, Spee, F., additional, Stefanini, A.M., additional, Stézowski, O., additional, Szilner, S., additional, Templeton, N., additional, Theisen, Ch., additional, Thiel, S., additional, Tomasi, F., additional, Tzvetkov, S., additional, Vigano, D., additional, Viscione, E., additional, Wieland, O., additional, Wimmer, K., additional, Wittwer, G., additional, and Zielińska, M., additional

Published
2023
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6. Conceptual design of the AGATA 2 pi array at LNL

Author

Valiente-Dobon, J. J., Menegazzo, R., Goasduff, A., Agguiaro, D., Aguilera, P., Angelini, F., Balogh, M., Bazzacco, D., Benito, J., Benzoni, G., Bez, N., Bolognesi, M., Bottoni, S., Brugnara, D., Carollo, S., Cocconi, P., Cogo, A., Collado, J., Crespi, F. C. L., Ertoprak, A., Escudeiro, R., Galtarossa, F., Gamba, E. R., Gambalonga, A., Gongora Servin, B., Gottardo, A., Gozzelino, A., Gulmini, M., Huang, Z., Marchi, T., Mengoni, D., Modanese, P., Napoli, D. R., Pellumaj, J., Perez-Vidal, R. M., Pigliapoco, S., Pilotto, E., Ramina, L., Rampazzo, M., Raniero, W., Rebeschini, M., Rezynkina, K., Rosso, D., Scarcioffolo, M., Scarpa, D., Sedlak, M., Smith, R., Toniolo, N., Veronese, F., Volpe, V., Zago, L., Zanon, I., Zhang, G., Abels, R., Allegrini, M. L., Aufranc, C., Baulieu, G., Belkhiria, C., Benettoni, M., Benini, D., Bentley, M., Biasotto, M., Blaizot, M., Miquel, J. Blasco, Boiano, C., Boston, A., Boston, H., Boujrad, A., Bourgault, P., Bracco, A., Brambilla, S., Burrows, I., Camera, F., Capra, S., Capsoni, A., Cash, R., Civera, J. V., Clement, E., Coelli, S., Cordwell, M., Corradi, L., Coudert, S., De Angelis, G., De Ruvo, L., Debras, G., Del Fabbro, M., Diklic, J., Dosme, N., Duchene, G., Duclos, B., Dudouet, J., Eberth, J., Elloumi, S., Everett, C., Fantinel, S., Fillinger, M., Fioretto, E., Fransen, C., Gadea, A., Gibelin, L., Gonzalez, V., Goupil, J., Goergen, C., Grant, A., Green, K., Ha, J., Hartnett, T., Henseler, K., Hess, H., Hirsch, R., Houarner, C., Jacob, J., Joannem, T., Judson, D. S., Karkour, N., Karolak, M., Kebbiri, M., Kieffer, J., Labiche, M., Lafay, X., Le Jeannic, P., Lefevre, A., Legay, E., Legruel, F., Lenzi, S., Leoni, S., Linget, D., Liptrot, M., Lopez-Martens, A., Lotode, A., Manara, L., Menager, L., Mijatovic, T., Million, B., Minarello, A., Montagnoli, G., Morrall, P., Mullacrane, I., Nyberg, Johan, Philippon, G., Polettini, M., Popieul, F., Pullia, A., Recchia, F., Reiter, P., Richardt, G., Rocchini, M., Roger, A., Saillant, F., Sanchis, E., Laskar, Md. S. R., Secci, G., Sigward, M. H., Simpson, J., Solenne, N., Spee, F., Stefanini, A. M., Stezowski, O., Szilner, S., Templeton, N., Theisen, Ch., Thiel, S., Tomasi, F., Tzvetkov, S., Vigano, D., Viscione, E., Wieland, O., Wimmer, K., Wittwer, G., Zielinska, M., Valiente-Dobon, J. J., Menegazzo, R., Goasduff, A., Agguiaro, D., Aguilera, P., Angelini, F., Balogh, M., Bazzacco, D., Benito, J., Benzoni, G., Bez, N., Bolognesi, M., Bottoni, S., Brugnara, D., Carollo, S., Cocconi, P., Cogo, A., Collado, J., Crespi, F. C. L., Ertoprak, A., Escudeiro, R., Galtarossa, F., Gamba, E. R., Gambalonga, A., Gongora Servin, B., Gottardo, A., Gozzelino, A., Gulmini, M., Huang, Z., Marchi, T., Mengoni, D., Modanese, P., Napoli, D. R., Pellumaj, J., Perez-Vidal, R. M., Pigliapoco, S., Pilotto, E., Ramina, L., Rampazzo, M., Raniero, W., Rebeschini, M., Rezynkina, K., Rosso, D., Scarcioffolo, M., Scarpa, D., Sedlak, M., Smith, R., Toniolo, N., Veronese, F., Volpe, V., Zago, L., Zanon, I., Zhang, G., Abels, R., Allegrini, M. L., Aufranc, C., Baulieu, G., Belkhiria, C., Benettoni, M., Benini, D., Bentley, M., Biasotto, M., Blaizot, M., Miquel, J. Blasco, Boiano, C., Boston, A., Boston, H., Boujrad, A., Bourgault, P., Bracco, A., Brambilla, S., Burrows, I., Camera, F., Capra, S., Capsoni, A., Cash, R., Civera, J. V., Clement, E., Coelli, S., Cordwell, M., Corradi, L., Coudert, S., De Angelis, G., De Ruvo, L., Debras, G., Del Fabbro, M., Diklic, J., Dosme, N., Duchene, G., Duclos, B., Dudouet, J., Eberth, J., Elloumi, S., Everett, C., Fantinel, S., Fillinger, M., Fioretto, E., Fransen, C., Gadea, A., Gibelin, L., Gonzalez, V., Goupil, J., Goergen, C., Grant, A., Green, K., Ha, J., Hartnett, T., Henseler, K., Hess, H., Hirsch, R., Houarner, C., Jacob, J., Joannem, T., Judson, D. S., Karkour, N., Karolak, M., Kebbiri, M., Kieffer, J., Labiche, M., Lafay, X., Le Jeannic, P., Lefevre, A., Legay, E., Legruel, F., Lenzi, S., Leoni, S., Linget, D., Liptrot, M., Lopez-Martens, A., Lotode, A., Manara, L., Menager, L., Mijatovic, T., Million, B., Minarello, A., Montagnoli, G., Morrall, P., Mullacrane, I., Nyberg, Johan, Philippon, G., Polettini, M., Popieul, F., Pullia, A., Recchia, F., Reiter, P., Richardt, G., Rocchini, M., Roger, A., Saillant, F., Sanchis, E., Laskar, Md. S. R., Secci, G., Sigward, M. H., Simpson, J., Solenne, N., Spee, F., Stefanini, A. M., Stezowski, O., Szilner, S., Templeton, N., Theisen, Ch., Thiel, S., Tomasi, F., Tzvetkov, S., Vigano, D., Viscione, E., Wieland, O., Wimmer, K., Wittwer, G., and Zielinska, M.

Abstract

The Advanced GAmma Tracking Array (AGATA) has been installed at Laboratori Nazionali di Legnaro (LNL), Italy. In this installation, AGATA will consist, at the beginning, of 13 AGATA triple clusters (ATCs) with an angular coverage of 1n,and progressively the number of ATCs will increase up to a 2 pi angular coverage. This setup will exploit both stable and radioactive ion beams delivered by the Tandem-PIAVE-ALPI accelerator complex and the SPES facility. The new implementation of AGATA at LNL will be used in two different configurations, firstly one coupled to the PRISMA large-acceptance magnetic spectrometer and lately a second one at Zero Degrees, along the beam line. These two configurations will allow us to cover a broad physics program, using different reaction mechanisms, such as Coulomb excitation, fusion-evaporation, transfer and fission at energies close to the Coulomb barrier. These setups have been designed to be coupled with a large variety of complementary detectors such as charged particle detectors, neutron detectors, heavy-ion detectors, high-energy gamma-ray arrays, cryogenic and gasjet targets and the plunger device for lifetime measurements. We present in this paper the conceptual design, characteristics and performance figures of this implementation of AGATA at LNL.

Published
2023
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12. List of Contributors

Author

Abbott, L.K., primary, Alghamdi, S.A., additional, Allen, E.B., additional, Allen, M.F., additional, Antunes, P.M., additional, Bonfante, P., additional, Brzostek, E.R., additional, Cameron, D.D., additional, Clemmensen, K.E., additional, Davidson, S.J., additional, Deckert, R.J., additional, Dickie, I.A., additional, Donn, S., additional, Drigo, B., additional, Fernandez, C.W., additional, Field, K.J., additional, Finlay, R.D., additional, Gehring, C.A., additional, Giovannetti, M., additional, Hamel, C., additional, Hodge, A., additional, Jansa, J., additional, Johnson, N.C., additional, Jones, M.D., additional, Koide, R.T., additional, Koyama, A., additional, Kuyper, T.W., additional, Leake, J.R., additional, Lehmann, A., additional, Leifheit, E.F., additional, Miller, R.M., additional, Miransari, M., additional, Pena, R., additional, Phillips, R.P., additional, Pickles, B.J., additional, Plenchette, C., additional, Querejeta, J.I., additional, Read, D.J., additional, Rebel, K.T., additional, Rillig, M.C., additional, Salvioli, A., additional, Simard, S.W., additional, Smith, K.R., additional, Smits, M.M., additional, Soong, J.L., additional, Swaty, R.L., additional, Teste, F.P., additional, Treseder, K.K., additional, Verbruggen, E., additional, Volpe, V., additional, Wallander, H., additional, and Wilson, G.W.T., additional

Published
2017
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15. Can Blebbistatin block the hypertrophy status in the zebrafish ex vivo cardiac model?

Author

Bonvissuto, Davide, Ceci, M., Lauri, C., Volpe, V., Bertone, R., Cervia, D., Sette, Claudio, Gornati, R., and Romano, N.

Subjects
Settore BIO/16 - ANATOMIA UMANA, Blebbistatin, Cardiomegaly, 4 or More Rings, Heterocyclic Compounds, 4 or More Rings, Cardiac hyperplasy, Cardiac hypertrophy, Ex vivo model, Phenylephrine, Zebrafish, Animals, Humans, Pericardium, Heterocyclic Compounds, Molecular Medicine, Molecular Biology
Abstract

Ex-vivo simple models are powered tools to study cardiac hypertrophy. It is possible to control the activation of critical genes and thus test the effects of drug therapies before the in vivo tests. A zebrafish cardiac hypertrophy developed by 500 μM phenylephrine (PE) treatment in ex vivo culture has been demonstrated to activate the essential expression of the embryonal genes. These genes are the same as those described in several previous pieces of research on hypertrophic pathology in humans. The efficacy of the chemical drug Blebbistatin (BL) on hypertrophy induced ex vivo cultured hearts is studied in this research. BL can inhibit the myosins and the calcium wave in counteracting the hypertrophy status caused by PE. Samples treated with PE, BL and PE simultaneously, or pre/post-treatment with BL, have been analysed for the embryonal gene activation concerning the hypertrophy status. The qRTPCR has shown an inhibitory effect of BL treatments on the microRNAs downregulation with the consequent low expression of essential embryonal genes. In particular, BL seems to be effective in blocking the hyperplasia of the epicardium but less effective in myocardium hypertrophy. The model can make it possible to obtain knowledge on the transduction pathways activated by BL and investigate the potential use of this drug in treating cardiac hypertrophy in humans.

Published
2022
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17. The GALILEO γ-ray array at the Legnaro National Laboratories

Author

Goasduff, A., primary, Mengoni, D., additional, Recchia, F., additional, Valiente-Dobón, J.J., additional, Menegazzo, R., additional, Benzoni, G., additional, Barrientos, D., additional, Bellato, M., additional, Bez, N., additional, Biasotto, M., additional, Blasi, N., additional, Boiano, C., additional, Boso, A., additional, Bottoni, S., additional, Bracco, A., additional, Brambilla, S., additional, Brugnara, D., additional, Camera, F., additional, Capra, S., additional, Capsoni, A., additional, Cocconi, P., additional, Coelli, S., additional, Cortés, M.L., additional, Crespi, F.C.L., additional, de Angelis, G., additional, Egea, F.J., additional, Fanin, C., additional, Fantinel, S., additional, Gadea, A., additional, Gamba, E.R., additional, Gambalonga, A., additional, Gesmundo, C., additional, Gosta, G., additional, Gottardo, A., additional, Gozzelino, A., additional, Gregor, E.T., additional, Gulmini, M., additional, Ha, J., additional, Hadyńska-Klȩk, K., additional, Illana, A., additional, Isocrate, R., additional, Jaworski, G., additional, John, P.R., additional, Lenzi, S.M., additional, Leoni, S., additional, Lunardi, S., additional, Magalini, M., additional, Marchini, N., additional, Million, B., additional, Modamio, V., additional, Nannini, A., additional, Napoli, D.R., additional, Pasqualato, G., additional, Pellumaj, J., additional, Pérez-Vidal, R.M., additional, Pigliapoco, S., additional, Polettini, M., additional, Porzio, C., additional, Pullia, A., additional, Ramina, L., additional, Rampazzo, G., additional, Rampazzo, M., additional, Rebeschini, M., additional, Rezynkina, K., additional, Rocchini, M., additional, Romanato, M., additional, Rosso, D., additional, Saltarelli, A., additional, Scarcioffolo, M., additional, Siciliano, M., additional, Testov, D.A., additional, Tomasella, D., additional, Tomasi, F., additional, Toniolo, N., additional, Ur, C.A., additional, Ventura, S., additional, Veronese, F., additional, Viscione, E., additional, Volpe, V., additional, Wieland, O., additional, Zanon, I., additional, Ziliani, S., additional, Zhang, G., additional, and Bazzacco, D., additional

Published
2021
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21. The SPES facility at Legnaro National Laboratories

Author

Marchi, T, primary, Prete, G, additional, Gramegna, F, additional, Andrighetto, A, additional, Antonini, P, additional, Ballan, M, additional, Bellato, M, additional, Bellan, L, additional, Benini, D, additional, Bisoffi, G, additional, Bermudez, J, additional, Benzoni, G, additional, Bortolato, D, additional, Borgna, F, additional, Calore, A, additional, Canella, S, additional, Carturan, S, additional, Ciatara, N, additional, Cinausero, M, additional, Cocconi, P, additional, Cogo, A, additional, Conventi, D, additional, Conte, V, additional, Comunian, M, additional, Costa, L, additional, Corradetti, S, additional, Angelis, G De, additional, Martinis, C De, additional, Ruvo, P De, additional, Esposito, J, additional, Fagotti, E, additional, Fabris, D, additional, Favaron, P, additional, Fioretto, E, additional, Galatá, A, additional, Gelain, F, additional, Giacchini, M, additional, Giora, D, additional, Gottardo, A, additional, Gulmini, M, additional, Lollo, M, additional, Lombardi, A, additional, Manzolaro, M, additional, Maggiore, M, additional, Maniero, D, additional, Mastinu, P F, additional, Monetti, A, additional, Pasquato, F, additional, Pegoraro, R, additional, Pisent, A, additional, Poggi, M, additional, Pavinato, S, additional, Pranovi, L, additional, Pedretti, D, additional, Roncolato, C, additional, Rossignoli, M, additional, Sarchiapone, L, additional, Scarpa, D, additional, Valiente Dobón, J J, additional, Volpe, V, additional, Vescovo, A, additional, and Zafiropoulos, D, additional

Published
2020
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22. Microscopic Techniques Coupled to Molecular and Genetic Approaches to Highlight Cell-Type Specific Differences in Mycorrhizal Symbiosis

Author

Fiorilli V., Volpe V., and Balestrini R.

Subjects
cell specificity, laser microdissection, mycorrhizal symbiosis
Abstract

Mycorrhizal symbioses, i.e., the associations between plant roots and soil symbi- otic fungi, are an essential feature of the biology of most terrestrial plants in a wide range of terrestrial ecosystems. Both plant and fungal genes involved in the dialogue between the two partners and in the symbiotic lifestyle have been identified in different mycorrhizal interactions. However, a mycorrhizal root represents a complex heterogeneous mixture of different cell and tissue types of both symbiotic partners, which complicates the interpretation of whole root expression profiling. Thus, examination of the process at the molecular level suffers from the use of whole organs since cell-type-specific differences might be masked. The aim of this chapter is to describe the main approaches that have been used to study cell specificity in mycorrhizal interactions, mainly considering AM symbiosis on which most of the experiments have been performed. Attention will be focused on the relevant advancements in knowledge obtained thanks to the application of microscopic techniques coupled to molecular and genetic approaches, such as in situ hybridization, approaches involving plant transforma- tion, and laser microdissection (LMD), to study cell specificity in symbiotic tissues.

Published
2019

25. The Association With Two Different Arbuscular Mycorrhizal Fungi Differently Affects Water Stress Tolerance in Tomato

Author

Volpe V., Chitarra, Cascone P., Volpe M.G., Bartolini P., Moneti G., Pieraccini G., Di Serio C., Maserti B., Guerrieri E., and Balestrini R.

Subjects
0106 biological sciences, 0301 basic medicine, volatile organic compound, arbuscular mycorrhizal symbiosis, Biofertilizer, Plant Science, Biology, lcsh:Plant culture, 01 natural sciences, 03 medical and health sciences, Nutrient, Symbiosis, Solanum lycopersicum, Botany, lcsh:SB1-1110, Cultivar, Original Research, water deficit, 2. Zero hunger, Abiotic component, Aphid, fungi, food and beverages, 15. Life on land, Arbuscular mycorrhizal symbiosis, Phosphate transporter, Plant tolerance, Volatile organic compound, Water deficit, biology.organism_classification, 030104 developmental biology, aphid, plant tolerance, Organic farming, phosphate transporter, Plant nutrition, 010606 plant biology & botany
Abstract

Arbuscular mycorrhizal (AM) fungi are very widespread, forming symbiotic associations with similar to 80% of land plant species, including almost all crop plants. These fungi are considered of great interest for their use as biofertilizer in low-input and organic agriculture. In addition to an improvement in plant nutrition, AM fungi have been reported to enhance plant tolerance to important abiotic and biotic environmental conditions, especially to a reduced availability of resources. These features, to be exploited and applied in the field, require a thorough identification of mechanisms involved in nutrient transfer, metabolic pathways induced by single and multiple stresses, physiological and eco-physiological mechanisms resulting in improved tolerance. However, cooperation between host plants and AM fungi is often related to the specificity of symbiotic partners, the environmental conditions and the availability of resources. In this study, the impact of two AM fungal species (Funneliformis mosseae and Rhizophagus intraradices) on the water stress tolerance of a commercial tomato cultivar (San Marzano nano) has been evaluated in pots. Biometric and eco-physiological parameters have been recorded and gene expression analyses in tomato roots have been focused on plant and fungal genes involved in inorganic phosphate (Pi) uptake and transport. R. intraradices, which resulted to be more efficient than F. mosseae to improve physiological performances, was selected to assess the role of AM symbiosis on tomato plants subjected to combined stresses (moderate water stress and aphid infestation) in controlled conditions. A positive effect on the tomato indirect defense toward aphids in terms of enhanced attraction of their natural enemies was observed, in agreement with the characterization of volatile organic compound (VOC) released. In conclusion, our results offer new insights for understanding the molecular and physiological mechanisms involved in the tolerance toward water deficit as mediated by a specific AM fungus. Moreover, they open new perspectives for the exploitation of AM symbiosis to enhance crop tolerance to abiotic and biotic stresses in a scenario of global change.

Published
2018
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36. A rice GRAS gene has an impact on the success of arbuscular mycorrhizal colonization

Author

Fiorilli V., Volpe V., Zanini S., Vallino M., Abbà S., and Bonfante P.

Subjects
GRAS Proteins, Arbuscular Mycorrhizal Symbiosis, fungi, food and beverages, Rice, Transcription Factors
Abstract

Arbuscular mycorrhiza (AM) is one of the most spread symbiosis established between 80% of land plants and soil fungi belonging to the Glomeromycota. Molecular determinants involved in the formation of arbuscular mycorrhizas are still poorly understood. It has been demonstrated that in both Legumes and rice plants, several GRAS transcription factors are directly involved in both mycorrhizal signaling and colonization, namely NSP1, NSP2, RAM1, DELLA, DELLA-interacting protein (DIP1) and RAD1. Here, we focused on a rice GRAS protein, named Arbuscular Mycorrhizal 18 (OsAM18), previously identified as specifically expressed in rice mycorrhizal roots, and considered as an AM-specific gene. Phylogenetic analysis revealed that OsAM18 had a peculiar amino acid sequence, which clustered with putative SCARECROW proteins, even though it formed a separate branch. Allelic osma18 mutant displayed a drastic reduction in mycorrhizal colonization in-tensity and in arbuscule abundance, as mirrored by OsPT11 expression level. Non-mycorrhizal osam18 plants displayed a comparable plant development and root apparatus compared with the WT, while mycorrhizal osam18 mutants showed a reduction of plant biomass compared with mycorrhizal WT plants. The results suggest that OsAM18 is a rice protein, which is likely to have an impact not only on the colonization process and AM functionality, but also on the systemic effects of the AM symbiosis.

Published
2015
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37. Toward the Elucidation of the Competing Role of Evaporation and Thermal Decomposition in Ionic Liquids: A Multitechnique Study of the Vaporization Behavior of 1-Butyl-3-methylimidazolium Hexafluorophosphate under Effusion Conditions

Author

Volpe, V., Brunetti, B., Gigli, G., Lapi, A., Vecchio Ciprioti, S., and Ciccioli, A.

Abstract

The evaporation/decomposition behavior of the imidazolium ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate (BMImPF6) was investigated in the overall temperature range 425–551 K by means of the molecular-effusion-based techniques Knudsen effusion mass loss (KEML) and Knudsen effusion mass spectrometry (KEMS), using effusion orifices of different size (from 0.2 to 3 mm in diameter). Specific effusion fluxes measured by KEML were found to depend markedly on the orifice size, suggesting the occurrence of a kinetically delayed evaporation/decomposition process. KEMS experiments revealed that other species are present in the vapor phase besides the intact ion pair BMImPF6(g) produced by the simple evaporation BMImPF6(l) = BMImPF6(g), with relative abundances depending on the orifice sizethe larger the orifice, the larger the contribution of the BMImPF6(g) species. By combining KEML and KEMS results, the conclusion is drawn that in the investigated temperature range, when small effusion orifices are used, a significant part of the mass loss/volatility of BMImPF6is due to molecular products formed by decomposition/dissociation processes rather than to evaporated intact ion pairs. Additional experiments performed by nonisothermal thermogravimetry-differential thermal analysis (TG-DTA) further support the evidence of simultaneous evaporation/decomposition, although the conventional decomposition temperature derived from TG curves is much higher than the temperatures covered in effusion experiments. Partial pressures of the BMImPF6(g) species were derived from KEMS spectra and analyzed by second- and third-law methods giving a value of ΔevapH298K°= 145.3 ± 2.9 kJ·mol–1for the standard evaporation enthalpy of BMImPF6. A comparison is done with the behavior of the 1-butyl-3-methylimidazolium bis(trifluoromethyl)sulfonylimide (BMImNTf2) ionic liquid.

Published
2024
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46. Crack path in a Zn-Cu-Al PE alloy under uniaxial load

Author

DI COCCO, Vittorio, Iacoviello, Francesco, Tomassi, L., Rossi, Alessandra, Natali, S., and Volpe, V.

Subjects
Zn-Cu-Al alloy, Pseudoeleastic behaviour, Crack micromechanisms, Zn-Cu-Al alloy, Pseudoeleastic behaviour, Crack micromechanisms
Published
2013

47. Crack initiation and growth in an Zn-Cu-Al PE alloy

Author

Vittorio Di Cocco, Iacoviello, F., Tomassi, L., Natali, S., and Volpe, V.

Subjects
Pseudo-Elastic Behaviour, Fracture, Shape Memory Alloy, CuZnAl alloy, Shape Memory Alloy, Pseudo-Elastic Behaviour, Fracture, CuZnAl alloy
Published
2013

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