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222 results on '"Volovitch P"'

5. Control of Protein Activity and Gene Expression by Cyclofen‐OH Uncaging

Author

Zhang, Weiting, Hamouri, Fatima, Feng, Zhiping, Aujard, Isabelle, Ducos, Bertrand, Ye, Shixin, Weiss, Shimon, Volovitch, Michel, Vriz, Sophie, Jullien, Ludovic, and Bensimon, David

Subjects
Genetics, Biotechnology, Generic health relevance, Animals, Gene Expression Regulation, Green Fluorescent Proteins, Humans, Ligands, Optogenetics, Polycyclic Compounds, Receptors, Estrogen, Recombinant Fusion Proteins, caged compounds, cyclofen-OH, gene expression, optogenetics, photolysis, Medicinal and Biomolecular Chemistry, Biochemistry and Cell Biology, Organic Chemistry
Abstract

The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. Whereas many of these approaches use fusion between a light-activable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly, and locally in a live organism. We present that approach and its uses in a variety of physiological contexts.

Published
2018

8. Optical Control of Tumor Induction in the Zebrafish.

Author

Feng, Zhiping, Nam, Suzy, Hamouri, Fatima, Aujard, Isabelle, Ducos, Bertrand, Vriz, Sophie, Volovitch, Michel, Jullien, Ludovic, Lin, Shuo, Weiss, Shimon, and Bensimon, David

Subjects
Animals, Zebrafish, Humans, Neoplasms, Cell Transformation, Neoplastic, Disease Models, Animal, Gene Expression Regulation, Neoplastic, Mutation, Oncogenes, Proto-Oncogene Proteins p21(ras), Transcriptional Activation, Biomarkers, Tumor, Cell Transformation, Neoplastic, Disease Models, Animal, Gene Expression Regulation, Biomarkers, Tumor, Cancer, Genetics, 2.1 Biological and endogenous factors, 1.1 Normal biological development and functioning, Generic Health Relevance, Biochemistry and Cell Biology, Other Physical Sciences
Abstract

The zebrafish has become an increasingly popular and valuable cancer model over the past few decades. While most zebrafish cancer models are generated by expressing mammalian oncogenes under tissue-specific promoters, here we describe a method that allows for the precise optical control of oncogene expression in live zebrafish. We utilize this technique to transiently or constitutively activate a typical human oncogene, kRASG12V, in zebrafish embryos and investigate the developmental and tumorigenic phenotypes. We demonstrate the spatiotemporal control of oncogene expression in live zebrafish, and characterize the different tumorigenic probabilities when kRASG12V is expressed transiently or constitutively at different developmental stages. Moreover, we show that light can be used to activate oncogene expression in selected tissues and single cells without tissue-specific promoters. Our work presents a novel approach to initiate and study cancer in zebrafish, and the high spatiotemporal resolution of this method makes it a valuable tool for studying cancer initiation from single cells.

Published
2017

9. Nimodipine fosters remyelination in a mouse model of multiple sclerosis and induces microglia-specific apoptosis

Author

Schampel, Andrea, Volovitch, Oleg, Koeniger, Tobias, Scholz, Claus-Jürgen, Jörg, Stefanie, Linker, Ralf A, Wischmeyer, Erhard, Wunsch, Marie, Hell, Johannes W, Ergün, Süleyman, and Kuerten, Stefanie

Subjects
Biomedical and Clinical Sciences, Immunology, Multiple Sclerosis, Neurosciences, Neurodegenerative, Autoimmune Disease, Brain Disorders, 2.1 Biological and endogenous factors, 1.1 Normal biological development and functioning, Neurological, Animals, Apoptosis, Calcium Channels, L-Type, Cells, Cultured, Disease Models, Animal, Encephalomyelitis, Autoimmune, Experimental, Mice, Microglia, Nimodipine, Nitric Oxide Synthase Type II, Oligodendroglia, Reactive Oxygen Species, Remyelination, Spinal Cord, EAE, microglia, MS, neuroprotection, nimodipine
Abstract

Despite continuous interest in multiple sclerosis (MS) research, there is still a lack of neuroprotective strategies, because the main focus has remained on modulating the immune response. Here we performed in-depth analysis of neurodegeneration in experimental autoimmune encephalomyelitis (EAE) and in in vitro studies regarding the effect of the well-established L-type calcium channel antagonist nimodipine. Nimodipine treatment attenuated clinical EAE and spinal cord degeneration and promoted remyelination. Surprisingly, we observed calcium channel-independent effects on microglia, resulting in apoptosis. These effects were cell-type specific and irrespective of microglia polarization. Apoptosis was accompanied by decreased levels of nitric oxide (NO) and inducible NO synthase (iNOS) in cell culture as well as decreased iNOS and reactive oxygen species levels in EAE. In addition, increased numbers of Olig2+APC+ oligodendrocytes were detected. Overall, nimodipine application seems to generate a favorable environment for regenerative processes and therefore could be a treatment option for MS, because it combines features of immunomodulation with beneficial effects on neuroregeneration.

Published
2017

20. Corrosion challenges towards a sustainable society

Author

Bender, R, Feron, D, Mills, D, Ritter, S, Bäßler, R, Bettge, D, De Graeve, I, Dugstad, A, Grassini, S, Hack, T, Halama, M, Han, Eh, Harder, T, Gareth, H, Kittel, J, Krieg, R, Leygraf, C, Martinelli, Arjan, Mol, A, Neff, D, Nilsson, Jo, Odnevall, I, Paterson, S, Paul, S, Prošek, T, Raupach, M, Revilla, Ir, Ropital, F, Schweigart, H, Szala, E, Terryn, H., Tidblad, J, Virtanen, S, Volovitch, P, Watkinson, D, Wilms, M, Winning, G, Zheludkevich, M, Materials and Chemistry, Electrochemical and Surface Engineering, and Materials and Surface Science & Engineering

Subjects
corrosion costs, corrosion, corrosion protection, preventive strategies, Mechanics of Materials, Mechanical Engineering, Materials Chemistry, Metals and Alloys, Environmental Chemistry, General Medicine, Surfaces, Coatings and Films
Abstract

Materials and corrosion (2022). doi:10.1002/maco.202213140, Published by Wiley-VCH, Weinheim [u.a.]

Published
2022
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41. Importance of atmospheric aerosol pollutants on the degradation of Al2O3 encapsulated Al-doped zinc oxide window layers in solar cells

Author

Zhang, S. -T, Maltseva, A., Herting, Gunilla, Guillemoles, J. -F, Schneider, N., Odnevall, Inger, Volovitch, P., Zhang, S. -T, Maltseva, A., Herting, Gunilla, Guillemoles, J. -F, Schneider, N., Odnevall, Inger, and Volovitch, P.

Abstract

Atmospheric aerosol pollutants are considered for the first time for the durability evaluation of non-metallic photovoltaic materials on the example of pristine and Al2O3-encapsulated Al-doped zinc oxide (AZO) window layers. The AZO samples were exposed to a varied temperature and humidity cycle, completed or not by a daily deposition of (NH4)2SO4 or NaCl aerosols, typical pollutants in rural and marine environments, respectively. The samples exposed with and without the pollutants were compared after 1 and 2 weeks of the test. Optical transmittance and conductivity significantly degraded only for the samples exposed with the pollutants. Raman spectroscopy, X-ray diffraction and X-ray photoelectron spectroscopy evidenced localized dissolution of the AZO film and chemical modification of the Al2O3 encapsulation. The most severe degradation was caused by (NH4)2SO4, which was attributed to the high stability of soluble [Zn (NH3)42+] complexes. The Al2O3 encapsulation improved chemical and physical stability of AZO in the presence of (NH4)2SO4 but not in the presence of NaCl. The latter can be explained by pitting corrosion of Al2O3. Optical transmission curves are coherent with the AZO layer thinning in the presence of NaCl and very localized AZO dissolution (most likely grain boundary etching) in the presence of (NH4)2SO4. The enhanced degradation of encapsulated AZO in the presence of atmospheric aerosol pollutants suggests that they cannot be neglected in the evaluation of barrier protection capacities of novel encapsulates and, more generally, in the outdoor durability assessment of novel photovoltaic materials and devices., QC 20220919

Published
2022
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42. Corrosion challenges towards a sustainable society

Author

Bender, R., Féron, D., Mills, D., Ritter, S., Bäßler, R., Bettge, D., De Graeve, I., Dugstad, A., Grassini, S., Hack, T., Halama, M., Han, E. -H, Harder, T., Hinds, G., Kittel, J., Krieg, R., Leygraf, Christopher, Martinelli, L., Mol, A., Neff, D., Nilsson, J. -O, Odnevall, Inger, Paterson, S., Paul, S., Prošek, T., Raupach, M., Revilla, R. I., Ropital, F., Schweigart, H., Szala, E., Terryn, H., Tidblad, J., Virtanen, S., Volovitch, P., Watkinson, D., Wilms, M., Winning, G., Zheludkevich, M., Bender, R., Féron, D., Mills, D., Ritter, S., Bäßler, R., Bettge, D., De Graeve, I., Dugstad, A., Grassini, S., Hack, T., Halama, M., Han, E. -H, Harder, T., Hinds, G., Kittel, J., Krieg, R., Leygraf, Christopher, Martinelli, L., Mol, A., Neff, D., Nilsson, J. -O, Odnevall, Inger, Paterson, S., Paul, S., Prošek, T., Raupach, M., Revilla, R. I., Ropital, F., Schweigart, H., Szala, E., Terryn, H., Tidblad, J., Virtanen, S., Volovitch, P., Watkinson, D., Wilms, M., Winning, G., and Zheludkevich, M.

Abstract

A global transition towards more sustainable, affordable and reliable energy systems is being stimulated by the Paris Agreement and the United Nation's 2030 Agenda for Sustainable Development. This poses a challenge for the corrosion industry, as building climate-resilient energy systems and infrastructures brings with it a long-term direction, so as a result the long-term behaviour of structural materials (mainly metals and alloys) becomes a major prospect. With this in mind “Corrosion Challenges Towards a Sustainable Society” presents a series of cases showing the importance of corrosion protection of metals and alloys in the development of energy production to further understand the science of corrosion, and bring the need for research and the consequences of corrosion into public and political focus. This includes emphasis on the limitation of greenhouse gas emissions, on the lifetime of infrastructures, implants, cultural heritage artefacts, and a variety of other topics., QC 20230404

Published
2022
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