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the most effective types of control over competitive activities is expert evaluation. Purpose of the study is to develop a methodology for expert assessment of competitive activities of highly qualified field hockey players. Material and methods. The study involved 16 highly qualified field hockey players, aged from 19 to 37 years. The average age of the players is 27.3±5.06 years. Sports Qualification: Master of sports of Ukraine. All players were given informed consent to participate in the study. The study was conducted in 2024 during the 2024 Olympic field hockey qualification competition (the city of Valencia, Spain). The research was carried out on the basis of the following methods: theoretical analysis and generalization of scientific and methodological literature; pedagogical observation; video recording of competitive activities; method of expert assessments; methods of Mathematical Statistics. Results. The author's methodology for expert assessment of competitive activity of field hockey players has been developed based on a 10-point scale, consisting of 5 criteria in the ball ownership phase and 5 criteria in the ball picking up phase. Each of the criteria is rated from 1 to 10 points. The fluctuation of the average values of the expert assessment of players of the national team of Ukraine in the matches of the Olympic qualification 2024 is in the range from 4.2±0.45 to 6.6±0.49 points. Conclusions. The developed methodology of expert assessment of competitive activity of highly qualified field hockey players is appropriate for effective management influences in the process of competitive activity of club and national teams in the Olympic sport – field hockey.

The subject of the study is the segmental manifestation of the rule of law principle and its elements in the activities of justice authorities, courts and public associations, taking into account the economic basis of its influence. The purpose of the study is to examine the forms of manifestation of the rule of law principle and its individual elements in the activities of justice and court authorities, to clarify the role of the public in its practical application, and to identify opportunities for its economic support. Methodology. The study used general scientific and special methods of scientific knowledge: the method of system analysis, the dialectical method, the formal logical method, the structural and functional method, as well as a number of empirical methods. In particular, the method of comparison was used to determine the transformation of penitentiary bodies and their transformation into penitentiary justice bodies. The results of the study demonstrated the need for: qualitative consideration of the aspect of ensuring the correction of a convicted person after serving his or her sentence; involvement of representatives of civil society institutions in the resocialisation processes; and identification of potential ways to implement effective reforms that can increase the efficiency and fairness of the judiciary. Conclusion. The article reveals the relationship between the transformation of the penal system into the penitentiary system and the implementation of the rule of law in this area and its comprehensive enforcement. It is noted that the criminal executive system takes into account the aspect of ensuring the serving of sentences, and in the penitentiary system, which operates under the rule of law, the authors additionally identifies such a qualitative component as ensuring the correction of a convicted person after serving his/her sentence and his/her resocialisation. It was found that the economic rationale for the transformation of the judiciary based on the rule of law consists in reducing the costs of: court functioning due to the possibility of digitalization of the preparatory stages before the court hearing and the holding of court hearings in videoconference mode; reducing costs for the court apparatus through optimization of its functions; reducing the number of court cases due to active mediation and other possible options for resolving disputes peacefully, etc. It is noted that justice in judicial proceedings means not only formal compliance with the law, but also the exercise of rights and freedoms of individuals with due regard for equality, objectivity and impartiality. The authors prove that non-governmental organisations are an important institution capable of promoting the principle of justice as an integral element of the rule of law, since they ensure the right to apply to court and the right to protection of members and third parties. The role of the public in shaping the economic basis for reforms and the proper functioning of state bodies was also highlighted. It is noted that national legislation needs to be amended in terms of granting NGOs the right to apply to court in the interests of third parties, in particular, in cases where a person is unable to protect his or her rights for valid reasons. The study analyses the fundamental provisions on economic support for the implementation of the rule of law in various spheres of public relations.

Abstract Background The aim of the study was to develop a technique for quantitative determination of rat urine metabolites by HPLC–MS/MS, which can be used to search for biomarkers of acute intoxication with organophosphates (OPs). Results The content of metabolites in the urine of rats exposed to a single dose of paraoxon (POX1x); interval, twice daily administration of paraoxon (POX2x); exposure to 2-(o-cresyl)-4H-1, 3, 2-benzodioxaphosphorin-2-oxide and paraoxon (CBPOX) was investigated. New data were obtained on the content in the urine of intact rats as well as rats in 3 models of OP poisoning: 3-methylhistidine, threonine, creatine, creatinine, lactic acid, acetylcarnitine, inosine, hypoxanthine, adenine, 3-hydroxymethyl-butyrate and 2-hydroxymethyl-butyrate. Conclusions The proposed assay procedure is a simple and reliable tool for urine metabolomic studies. Within 1–3 days after OP exposure in all three models of acute intoxication, the concentration of metabolites in rat urine, with the exception of adenine, changes similarly and symmetrically, regardless of the method of poisoning modeling, in all three models of acute intoxication. Further studies are needed to determine the specificity and reliability of using urinary metabolite concentration changes as potential biomarkers of acute organophosphate intoxication. Graphical Abstract

Despite playing diverse physiological roles, the area surrounding the central canal, lamina X, remains one of the least studied spinal cord regions. Technical challenges and limitations of the commonly used experimental approaches are the main difficulties that hamper lamina X research. In the current protocol, we describe a reliable method for functional investigation of lamina X neurons that requires neither time-consuming slicing nor sophisticated in vivo experiments. Our approach relies on ex vivo hemisected spinal cord preparation that preserves the rostrocaudal and mediolateral spinal architecture as well as the dorsal roots, and infrared LED oblique illumination for visually guided patch clamp in thick blocks of tissue. When coupled with electric stimulation of the spared dorsal roots, electrophysiological recordings provide information on primary afferent inputs to lamina X neurons from myelinated and non-myelinated fibers and allow estimating primary afferent–driven presynaptic inhibition. Overall, we describe a simple, time-efficient, inexpensive, and versatile approach for lamina X research.

The article presents the results of investigations of the decay coefficients of dense plasma in impulse discharges in water initiated by an electric explosion of an iron conductor. An analysis of time-resolved and spatial emission spectra of the discharges was performed, along with the study of absorption spectra dynamics with a 5 μs interval. The electron concentration of the dense plasma was determined at different time points, allowing the determination of its decay coefficients at electron concentrations ranging from 1020 to 1021 cm-3. It was found that under equal electron concentrations in the plasma, the decay coefficients of plasma with iron impurities are consistent with the decay coefficients of plasma with tungsten impurities.

Interaction between a fixed point electric charge Q and a freely rotating point electric dipole with the magnitude P pinned near a plane interface between two dispersionless insulators with different dielectric permittivities ɛ1 and ɛ2 has been considered. It was shown that, as a result of this interaction and the interaction of the dipole with the polarization charges induced at the interface by the charge Q and the dipole itself, there arise regions where the dipole can possess either one or two equilibrium orientations. The spatial distributions of the electrostatic dipole energy Wtotal under the combined action of the charge Q and the induced interface polarization charges, as well as the equilibrium dipole orientations (orientation maps), the boundaries between the regions with different numbers of dipole orientations, and their evolution with the variation of problem parameters (the charge and dipole magnitudes, the mismatch between ɛ1 and ɛ2, and the charge–interface distance) were calculated. It was shown that there can emerge local minima of Wtotal, which may play the role of traps for dipoles (in particular, excitons in layered structures), and the corresponding requirements for the problem parameters were found. Most results were obtained in analytical form. The model can be applied to various physical systems, for instance, polar molecules, excitons, and trions in layered structures. [ABSTRACT FROM AUTHOR]

The purpose of the study was to experimentally substantiate the methodological approach to the development of a comprehensive assessment of the preparedness of highly qualified field hockey players at the stage of direct preparation for the main competitions. Materials and methods. Highly qualified players, members of the national field hockey team of Ukraine (n=21) participated in the study. The average age of the players was (х ± S) 24.9 ± 5.76 years, their sports qualification was masters of sports of Ukraine. The study was conducted over the first and second competitive periods of the two-cycle planning of the training process of highly qualified field hockey players of the 2020-2021 sports season. During the first competitive period, a methodical approach to comprehensive assessment of the preparedness of highly qualified field hockey players was developed. Within the second competition period, which included the stage of direct preparation for the main competitions indicators of a comprehensive assessment of readiness of the players were determined. In order to develop a comprehensive assessment of fitness, tests and criteria were used to assess the level of physical, functional fitness, physical fitness in relation to technique and competitive activity of hockey players. Results. A methodical approach has been developed for the comprehensive assessment of the preparedness of highly qualified field hockey players according to the following algorithm: determination of criteria for the manifestation of physical and functional preparedness, physical preparedness in relation to technique, competitive activity; development of a 10-point rating scale; establishment of a comprehensive assessment of players’ readiness during the stage of direct preparation for the main competitions. Conclusions. The structure of the stage of direct preparation for the main competitions consisted of a basic and a special preparatory mesocycles. The volume of direct motor activity was 38 hours, in particular, the ratio of means: general preparatory exercises – 55.4%, special preparatory exercises – 19.5%, lead exercises – 10.9%, competitive exercises – 15.2%; ratio of training loads: aerobic – 56.5%, aerobic-anaerobic – 32.7%, anaerobic-lactate – 7.6%, anaerobic-glycolytic – 3.2%. A statistically probable positive change (р

The objective of this study was the additive microplasma powder deposition of 3D metal products. The regularities of the influence of technological parameters of additive microplasma deposition of spatial objects using the powder filler material of a Fe–Cr–Ni–B–Si system on the formation of the microstructure and stress–strain state of 3D product material were studied in this work. Product walls with a layered metal structure with a deposited layer height of about 650 µm and 3.0–3.5 mm thickness were formed as a result of additive microplasma deposition of the HYF–103 powder of a Fe–Cr–Ni–B–Si system. The deposited metal ensured a high ultimate strength (above 600 MPa). The finite element method was used to derive the solution of the thermomechanical problem of additive deposition of 3D prototypes («cylinder», «triangular prism», «square prism», «reverse cone», «straight cone») with HYF–103 powder. The equivalent stresses of the highest magnitude (565 MPa) were predicted in the model sample of the “reverse cone” type, and the lowest stresses (552 MPa) were present in the sample of the “straight cone” type. For all the models, the maximal values of radial movements corresponded to the range of 0.22–0.28 mm. The respective technological mode of deposition was selected to minimize the stress–strain state of the produced 3D objects.

The relevance of the topic is determined by the importance of the principle of political neutrality as a preventive factor in the professional activity of the prosecutor, which calls for ensuring him from the external influence of any political force and the appearance of personal illegal interests in social cataclysms). The purpose of the article – to consider the professional activity of the prosecutor through the understanding of its principles as indicative characteristics of professional skills and professionalism. To confirm this, the author chose functionalism as the main methodological approach, which gave him reasons to claim that the functions of activity determine professional skills (the profession of a prosecutor), and the principles define professionalism (level of mastery of this profession). As auxiliary methods were used: formal-logical (for an argumentative presentation of the research material), formal-dogmatic (for the analysis of current regulatory documents) and comparative-legal (for comparison of the analyzed material). The main results of the study are shown the principle of political neutrality as a requirement in the field of professional activity of civil servants not only in Ukraine, but also in the European Union, the integration with which is defined as the main vector of further development of our state. A substantive distinction is made between political neutrality and synonymously similar concepts of political impartiality and independence, apoliticality and non-partisanship. At the same time, political neutrality is shown as one of the most important criteria in the formation of requirements for professional selection, professional training, professional development and professional activities of prosecutors. The practical value of the study is a warning to prosecutors against political ignorance, which can arise from a misunderstanding of political neutrality. In order to avoid or overcome this negative aspect, a number of factors are proposed for the prosecutor to achieve professional skills, which is described through a peculiar formula: the prosecutor's professional skills can be considered the sum of political, media and informational and environmental literacy, divided by political neutrality

The process of aging is accompanied by a dynamic restructuring of the immune response, a phenomenon known as immunosenescence. Further, damage to the endothelium can be both a cause and a consequence of many diseases, especially in elderly people. The purpose of this study was to carry out immunological and biochemical profiling of elderly people with acute ischemic stroke (AIS), chronic cerebral circulation insufficiency (CCCI), prediabetes or newly diagnosed type II diabetes mellitus (DM), and subcortical ischemic vascular dementia (SIVD). Socio-demographic, lifestyle, and cognitive data were obtained. Biochemical, hematological, and immunological analyses were carried out, and extracellular vesicles (EVs) with endothelial CD markers were assessed. The greatest number of significant deviations from conditionally healthy donors (HDs) of the same age were registered in the SIVD group, a total of 20, of which 12 were specific and six were non-specific but with maximal differences (as compared to the other three groups) from the HDs group. The non-specific deviations were for the MOCA (Montreal Cognitive Impairment Scale), the MMSE (Mini Mental State Examination) and life satisfaction self-assessment scores, a decrease of albumin levels, and ADAMTS13 (a Disintegrin and Metalloproteinase with a Thrombospondin Type 1 motif, member 13) activity, and an increase of the VWF (von Willebrand factor) level. Considering the significant changes in immunological parameters (mostly Th17-like cells) and endothelial CD markers (CD144 and CD34), vascular repair was impaired to the greatest extent in the DM group. The AIS patients showed 12 significant deviations from the HD controls, including three specific to this group. These were high NEFAs (non-esterified fatty acids) and CD31 and CD147 markers of EVs. The lowest number of deviations were registered in the CCCI group, nine in total. There were significant changes from the HD controls with no specifics to this group, and just one non-specific with a maximal difference from the control parameters, which was α1-AGP (alpha 1 acid glycoprotein, orosomucoid). Besides the DM patients, impairments of vascular repair were also registered in the CCCI and AIS patients, with a complete absence of such in patients with dementia (SIVD group). On the other hand, microvascular damage seemed to be maximal in the latter group, considering the biochemical indicators VWF and ADAMTS13. In the DM patients, a maximum immune response was registered, mainly with Th17-like cells. In the CCCI group, the reaction was not as pronounced compared to other groups of patients, which may indicate the initial stages and/or compensatory nature of organic changes (remodeling). At the same time, immunological and biochemical deviations in SIVD patients indicated a persistent remodeling in microvessels, chronic inflammation, and a significant decrease in the anabolic function of the liver and other tissues. The data obtained support two interrelated assumptions. Taking into account the primary biochemical factors that trigger the pathological processes associated with vascular pathology and related diseases, the first assumption is that purine degradation in skeletal muscle may be a major factor in the production of uric acid, followed by its production by non-muscle cells, the main of which are endothelial cells. Another assumption is that therapeutic factors that increase the levels of endothelial progenitor cells may have a therapeutic effect in reducing the risk of cerebrovascular disease and related neurodegenerative diseases.

Introduction. Strenuous aerobic work inherent to cyclical sports requires adequate oxygenation of the athletes’ working muscles. One of the ways to improve the oxygen transport function of blood is to optimize the structural and functional state of erythrocytes, for example, by using succinic acid in the form of the medical drug Armadin Long. This pharmacological drug is widespread in clinical practice for treating hypoxic and ischemic conditions, and very little is known about its use for improving the condition of the erythrocytes in the blood of athletes, which determined the relevance of this study. The objective of the study was to assess the feasibility and effectiveness of using the medical drug Armadine Long to improve the state of the erythrocyte link of the blood oxygen transport system during aerobic exercise. Materials and Methods. A randomized blinded placebo-controlled trial included 40 male middle-distance runners (aerobic discipline of athletics). The subjects were divided into two subgroups matched for number, age, and anthropometric characteristics (strats). In this work, laboratory methods were used, including hematological tests (in particular, hemoglobin and erythrocytes level measurement and erythrocyte characteristics evaluation), as well as biochemical method: the study of prooxidant and antioxidant balance (activity of lipid peroxidation according to changes in the content of maloniс dialdehyde and antioxidant protection according to changes in the concentration of reduced glutathione) and functional characteristics of erythrocyte membranes (permeability, sorption capacity, sorption capacity of the glycocalyx) as well as evaluation of the main components of lipid and protein composition of red blood cell membranes. Pedagogical research methods were based on the determination of relative aerobic capacity using the generally accepted PWC170 test. Results of the study. During 21 days of intensive loads, an almost two-fold increase in contents of malonic dialdehyde was observed with a parallel decrease in reduced glutathione content by 23.5 % during aerobic loads. At the same time, there was a deterioration of the functional characteristics of erythrocytes and a decrease in the relative aerobic capacity in comparison with the baseline data. The use of the medical drug Armadine Long at a dose of 600 mg per day improved the indicated characteristics of erythrocytes. At the same time, there were positive changes in the protein and lipid state of the erythrocyte membranes, and the athletes’ aerobic power increased by 38.7%. It substantiates the high ergogenic ability of succinic acid, which is based on the normalization of the lipoperoxidation process and the improvement of the structural and functional characteristics of erythrocyte membranes.

During the initial diagnosis of urgent medical conditions, which include acute infectious diseases, it is important to assess the severity of the patient’s clinical state as quickly as possible. Unlike individual biochemical or physiological indicators, derived indices make it possible to better characterize a complex syndrome as a set of symptoms, and therefore quickly take a set of adequate measures. Recently, we reported on novel diagnostic indices containing butyrylcholinesterase (BChE) activity, which is decreased in COVID-19 patients. Also, in these patients, the secretion of von Willebrand factor (vWF) increases, which leads to thrombosis in the microvascular bed. The objective of this study was the determination of the concentration and activity of vWF in patients with COVID-19, and the search for new diagnostic indices. One of the main objectives was to compare the prognostic values of some individual and newly derived indices. Patients with COVID-19 were retrospectively divided into two groups: survivors (n = 77) and deceased (n = 24). According to clinical symptoms and computed tomography (CT) results, the course of disease was predominantly moderate in severity. The first blood sample (first point) was taken upon admission to the hospital, the second sample (second point)—within 4–6 days after admission. Along with the standard spectrum of biochemical indicators, BChE activity (BChEa or BChEb for acetylthiocholin or butyrylthiocholin, respectively), malondialdehyde (MDA), and vWF analysis (its antigen level, AGFW, and its activity, ActWF) were determined and new diagnostic indices were derived. The pooled sensitivity, specificity, and area under the receiver operating curve (AUC), as well as Likelihood ratio (LR) and Odds ratio (OR) were calculated. The level of vWF antigen in the deceased group was 1.5-fold higher than the level in the group of survivors. Indices that include vWF antigen levels are superior to indices using vWF activity. It was found that the index [Urea] × [AGWF] × 1000/(BChEb × [ALB]) had the best discriminatory power to predict COVID-19 mortality (AUC = 0.91 [0.83, 1.00], p < 0.0001; OR = 72.0 [7.5, 689], p = 0.0002). In addition, [Urea] × 1000/(BChEb × [ALB]) was a good predictor of mortality (AUC = 0.95 [0.89, 1.00], p < 0.0001; OR = 31.5 [3.4, 293], p = 0.0024). The index [Urea] × [AGWF] × 1000/(BChEb × [ALB]) was the best predictor of mortality associated with COVID-19 infection, followed by [Urea] × 1000/(BChEb × [ALB]). After validation in a subsequent cohort, these two indices could be recommended for diagnostic laboratories.

The urgency of the chosen research area is determined by the need to train specialists to work in an inclusive environment. The psychological and pedagogical literature on the functioning of inclusive education is analyzed and the current problems of this process are identified, which require solutions: the absence of principles of psychological and pedagogical support of children in the system of inclusive education; the lack of recommendations for involving parents in the educational process; unpreparedness of high institutions to prepare specialists to work in the conditions of inclusion; the need to update the system of professional training of future teachers taking into account the peculiarities of inclusive education; insufficient study of foreign experience, etc.Based on generalizations of theoretical analysis and own practical experience the ways of improvement of preparation of specialists for realization of individual educational path of children with special educational needs are substained: the improvement of educational programs of preparation of future specialists for work in the inclusive environment, renovation of the training methods of future teachers factor into neuropedagogical aspects, and also to make opportunities for professional self-improvement of specialists working in an inclusive environment during their professional activities.The need to take into account neuropedagogical aspects to ensure effective training of specialists to implement the individual educational path of children with special educational needs is based on the specific features of inclusive education, which requires knowledge of cognitive neurology, differential psychophysiology, neuropsychology and the formation of relevant skills.It seems promising the creation of a pattern for training specialists to implement the individual educational path of children with special educational needs, as well as the organization of a pedagogical experiment to test its effectiveness.

Introduction. The outbreak of coronavirus infection (COVID-19) continues to be one of the most serious problems of the mankind. Patients who develop pneumonia as a result of coronavirus infection require rehabilitation measures to restore the functional capacity of the body and to prevent the negative consequences of the disease. For patients with COVID‑19, rehabilitation should be aimed at alleviating symptoms (shortness of breath), improving psychological condition, physical form and quality of life. This can be achieved by improving the organization and methodology of pulmonary rehabilitation of patients with COVID-19. Purpose is to justify the use of pulmonary rehabilitation in patients with COVID-19. Materials and methods: analysis and systematization of data from modern scientific and methodological literature and Internet sources. Results. The emergence and spread of coronavirus (SARS-CoV-2) is a major public health issue. Post-COVID syndrome has already become a massive phenomenon and part of our lives. Recovery from coronavirus infection is necessary for all, regardless of the severity of the disease. Physical therapy can be used at various stages of treatment for COVID-19 patients. However, the decision to use it, the nature, scope and purpose of the interventions should be based on clinical evidence, the safety of patients and staff. Pulmonary rehabilitation, the main component of which is physical exercise (aerobic and/or resistance training), can play a vital role in the recovery of patients, improving physical fitness, muscle strength and quality of life of those infected with severe acute respiratory coronavirus syndrome. Moreover, under quarantine conditions, it was important for many patients to have access to home-based rehabilitation, which was provided by tele-rehabilitation facilities using telecommunications technology. Conclusions. Thus, pulmonary rehabilitation is an important part of recovery from COVID-19. The development of complications and the increase in the number of pulmonological patients who have suffered a severe or moderate coronary infection are updating the issues of adaptation of traditional rehabilitation programs to new conditions.

The study purpose is to establish differences in the joint activities of soccer teams of different age. Materials and methods. Psychological methods for indicators of joint activity of sports teams were used. Statistical analysis was performed using non-parametric analysis of variance (Kruskal-Wallis H-test). The study involved 105 soccer players, including 37 boys aged 15–18, 45 juniors aged 19–21 and 23 adult athletes aged 22 and over. Results. In the young soccer players, group interaction, intellectual communication, psychological compatibility, as well as indicators of real and reflective group subjectivity are higher than in the adult and junior teams. The levels of group organization, diligence and success in performing sports tasks are dominant in the adult teams. At the same time, the integrative characteristic of joint sports activities is group efficiency, which is presumably the most pronounced in adults, thus characterizing the processes within the mental organization of joint activities of soccer players. It is the result of their cooperation, a transformed form of external teamwork. Conclusions. The organization of joint mental activity differs in different age groups. This affects the formation and development of intra-group position of the athletes, influencing their optimal involvement in the team in the performance of designed or creative functions. Keywords: soccer players, efficiency, interaction, subjectivity, organization.

One of the main tasks of contemporary plant physiology is regulation of growth and development of cultivated plants in order to optimize the productive process. The scientific community focuses its attention on the use of natural activators and growth inhibitors. We investigated the effect of foliar treatment with 0.005% solution of gibberellic acid and 0.025% solution of the anti-gibberellic preparation tebuconazole on morphogenesis, leaf mesostructure, content of photosynthetic pigments, balance of endogenous phytobrybrides and lymphocyte B and productivity of tomatoes. The vegetation experiment was carried out in the conditions of soil-sand culture in vessels with a 10-liter volume. The treatment was carried out in the budding phase. Morphometric parameters were measured every 10 days. The mesostructure of the middle tier leaves was studied in the fruit formation phase, and the chlorophyll content was determined in the raw material by spectrophotometric method. Analytical determination of endogenous phytohormones – indolyl-3-acetic (IAA), gibberellic acid and abscisic (ABA) acids and cytokinins – zeatin (Z), zeatin-O-glucoside (ZG), zeatinribozide (Znila) and isopentenyladenosine (iPA) was performed by high performance liquid chromatography – mass spectrometry (HPLC-MS). With gibberellic acid treatment plant height increased significantly, while with tebuconazole it decreased. Gibberellic acid increased the number of leaves per plant, and tebuconazole did not change it. The preparations increased the number of leaf blades per leaf, the total number of leaf blades per plant, the weight of the raw material of leaves, the area of leaf blades and the area of the leaves at the end of the study period. The dry matter weight of stems and roots under the action of gibberellic acid increased, and during the treatment of tebuconazole decreased. Gibberellic acid increased the dry matter of the whole plant, and tebuconazole did not change it. Under the action of tebuconazole the content of chlorophyll in the leaves increased, while under the action of gibberellic acid it decreased. Both regulators increased the volume of columnar parenchyma cells. Gibberellic acid increased the size of spongy parenchyma cells, while tebuconazole did not change them. It is revealed that the action of exogenous gibberellic acid in stems and leaves increased the content of endogenous IAA and gibberellic acid, and tebuconazole decreased their content. The ABA content in stems and leaves increased with tebuconazole treatments and decreased with exogenous gibberellic acid. The total cytokinin content in the leaves was higher than in the stems in both the control and the experiment samples. Growth regulators induced an increase in the cytokinin pool in leaves and a decrease in stems. Gibberellic acid increased the content of all five forms of cytokinins in the leaves, and tebuconazole increased only two isoforms. In the stems under the action of both growth regulators the content of Z decreased and iP increased. The content of ZR and iPA in stems increased after the application of the retardant and decreased under the action of growth stimulant. The ZG content exceeded the control after gibberellic acid treatment and was in trace concentrations under the action of tebuconazole. Growth regulators optimized the productivity of tomato plants: under the action of gibberellic acid there was a considerable increase in the number of fruits per plant, and after the use of tebuconazole the average weight of one fruit significantly increased. The obtained results demonstrated that anatomical-morphological and structural-functional rearrangements in tomato plants under the action of exogenous gibberellic acid and tebuconazole occurred against the background of changes in the balance and distribution of endogenous hormones. Increased photosynthetic activity, stimulation of growth processes of some plant organs and inhibition of others increased the biological crop capacity.

The development of pain symptoms in peripheral diabetic neuropathy (PDN) is associated with the upregulation of T-type Ca2+ channels (T-channels) in the soma of nociceptive DRG neurons. Moreover, a block of these channels in DRG neurons effectively reversed mechanical and thermal hyperalgesia in animal diabetic models, indicating that T-channel functioning in these neurons is causally linked to PDN. However, no particular mechanisms relating the upregulation of T-channels in the soma of nociceptive DRG neurons to the pathological pain processing in PDN have been suggested. Here we have electrophysiologically identified voltage-gated currents expressed in nociceptive DRG neurons and developed a computation model of the neurons, including peripheral and central axons. Simulations showed substantially stronger sensitivity of neuronal excitability to diabetes-induced T-channel upregulation at the normal body temperature compared to the ambient one. We also found that upregulation of somatic T-channels, observed in these neurons under diabetic conditions, amplifies a single action potential invading the soma from the periphery into a burst of multiple action potentials further propagated to the end of the central axon. We have concluded that the somatic T-channel-dependent amplification of the peripheral nociceptive input to the spinal cord demonstrated in this work may underlie abnormal nociception at different stages of diabetes development.

• Spared nerve injury (SNI) altered the action potential (AP) output of lamina I spino-parabrachial neurons (SPNs) without affecting their resting potential or membrane resistance.• In one-third of SPNs, high-threshold dorsal root stimulation elicited persistent AP firing which was never observed in cells from naïve animals.• 38% of SPNs from SNI rats showed spontaneous persistent AP firing.• After SNI low- and high-output SPNs were no longer nociceptive-specific as part of them responded with APs to low-threshold stimulation.• These SNI-induced changes of SPN output might represent cellular mechanisms for neuropathy-associated allodynia, hyperalgesia, and spontaneous pain.

Although spinal processing of sensory information greatly relies on afferent-driven (AD) presynaptic inhibition (PI), our knowledge about how it shapes peripheral input to different types of nociceptive neurons remains insufficient. Here we examined the AD-PI of primary afferent input to spinal neurons in the marginal layer, lamina I, and the layer surrounding the central canal, lamina X; two nociceptive-processing regions with similar patterns of direct supply by Aδ- and C-afferents. Unmyelinated C-fibers were selectively activated by electrical stimuli of negative polarity that induced an anodal block of myelinated Aβ/δ-fibers. Combining this approach with the patch-clamp recording in an ex vivo spinal cord preparation, we found that attenuation of the AD-PI by the anodal block of Aβ/δ-fibers resulted in the appearance of new mono- and polysynaptic C-fiber-mediated excitatory postsynaptic current (EPSC) components. Such homosegmental Aβ/δ-AD-PI affected neurons in the segment of the dorsal root entrance as well as in the adjacent rostral segment. In their turn, C-fibers from the L5 dorsal root induced heterosegmental AD-PI of the inputs from the L4 Aδ- and C-afferents to the neurons in the L4 segment. The heterosegmental C-AD-PI was reciprocal since the L4 C-afferents inhibited the L5 Aδ- and C-fiber inputs, as well as some direct L5 Aβ-fiber inputs. Moreover, the C-AD-PI was found to control the spike discharge in spinal neurons. Given that the homosegmental Aβ/δ-AD-PI and heterosegmental C-AD-PI affected a substantial percentage of lamina I and X neurons, we suggest that these basic mechanisms are important for shaping primary afferent input to the neurons in the spinal nociceptive-processing network.