1. Genetic determinants of Biofilm formation of Helicobacter pylori using whole-genome sequencing
- Author
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Kartika Afrida Fauzia, Hafeza Aftab, Muhammad Miftahussurur, Langgeng Agung Waskito, Vo Phuoc Tuan, Ricky Indra Alfaray, Takashi Matsumoto, Michiyuki Yurugi, Phawinee Subsomwong, Evariste Tshibangu Kabamba, Junko Akada, and Yoshio Yamaoka
- Subjects
Whole-genome sequences ,SNP ,Variants ,Biofilm formation ,Antibiotic resistance ,Infectious disease ,Microbiology ,QR1-502 - Abstract
Abstract Background Infection with Helicobacter pylori as the cause of gastric cancer is a global public health concern. In addition to protecting germs from antibiotics, biofilms reduce the efficacy of H. pylori eradication therapy. The nucleotide polymorphisms (SNPs) related with the biofilm forming phenotype of Helicobacter pylori were studied. Results Fifty-six H. pylori isolate from Bangladeshi patients were included in this cross-sectional study. Crystal violet assay was used to quantify biofilm amount, and the strains were classified into high- and low-biofilm formers As a result, strains were classified as 19.6% high- and 81.4% low-biofilm formers. These phenotypes were not related to specific clades in the phylogenetic analysis. The accessories genes associated with biofilm from whole-genome sequences were extracted and analysed, and SNPs among the previously reported biofilm-related genes were analysed. Biofilm formation was significantly associated with SNPs of alpA, alpB, cagE, cgt, csd4, csd5, futB, gluP, homD, and murF (P
- Published
- 2023
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