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494 results on '"Vliegenthart, Johannes F. G."'

1. Unravelling Carbohydrate Interactions with Biosensors Using Surface Plasmon Resonance (SPR) Detection

Author

Haseley, Simon R., Kamerling, Johannis P., Vliegenthart, Johannes F. G., de Meijere, Armin, editor, Kessler, Horst, editor, Ley, Steven V., editor, Thiem, Joachim, editor, Vögtle, Fritz, editor, Houk, K. N., editor, Lehn, Jean-Marie, editor, Schreiber, Stuart L., editor, Trost, Barry M., editor, Yamamoto, Hisashi, editor, and Penadés, Soledad, editor

Published
2002
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9. Analysis of protein-carbohydrate interaction at the lower size limit of the protein part (15-Mer peptide) by NMR spectroscopy, electrospray ionization mass spectrometry, and molecular modeling

Author

Siebert, Hans-Christian, Shan-Yun Lu, Frank, Martin, Kramer, Jasper, Wechselberger, Rainer, Joosten, John, Andre, Sabine, Rittenhouse-Olson, Kate, Roy, Rene, Lieth, Claus-Wilhelm von der, Kaptein, Robert, Vliegenthart, Johannes F. G., Heck, Albert J. R., and Gabius, Hans-Joachim

Subjects
Biochemistry -- Research, Proteins -- Physiological aspects, Carbohydrates -- Physiological aspects, Nuclear magnetic resonance spectroscopy -- Usage, Ionization -- Physiological aspects, Mass spectrometry -- Usage, Peptides -- Physiological aspects, Biological sciences, Chemistry
Abstract

Research has been conducted on the carbohydrate-binding peptides. The reactivity of these peptides has been investigated and the results are presented.

Published
2002

15. Knowledge-based Homology Modeling and Experimental Determination of Amino Acid Side Chain Accessibility by the Laser Photo CIDNP (Chemically Induced Dynamic Nuclear Polarization) Approach in Solution: Lessons from the Small Sialidase of Clostridium perfringens

Author

Siebert, Hans-Christian, Tajkhorshid, Emadeddin, von der Lieth, Claus-Wilhelm, Kleineidam, Reinhard G., Kruse, Susanne, Schauer, Roland, Kaptein, Robert, Gabius, Hans-Joachim, and Vliegenthart, Johannes F. G.

Published
1996
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32. Complete enzymic synthesis of the mucin-type sialyl Lewis x epitope, involved in the interaction between PSGL-1 and P-selectin

Author

Zeng, Steffen, Gutiérrez Gallego, Ricardo, Dinter, Andre, Malissard, Martine, Kamerling, Johannis P, Vliegenthart, Johannes F G, Berger, Eric G, University of Zurich, and Berger, Eric G

Subjects
1307 Cell Biology, carbohydrates (lipids), 1303 Biochemistry, 1312 Molecular Biology, 610 Medicine & health, 142-005 142-005
Abstract

Sialyl Lewis x (sLex) is an established selectin ligand occurring on N- and O-linked glycans. Using a completely enzymic approach starting from p-nitrophenyl N-acetyl-α-D-galactosaminide (GalNAc(α1-pNp as core substrate, the sLex-oligosaccharide Neu5Ac(α2-3)Gal(β1-4)[Fuc(α1-3)]GlcNAc(β1-6)[Gal(β1-3)]GalNAc(α1-pNp, representing the O-linked form, was synthesized in an overall yield of 32%. In a first step, Gal(β1-3)GalNAc(α1-pNp was prepared in a yield of 52% using UDP-Gal and an enriched preparation of β3-galactosyltransferase (EC 2.4.1.122) from rat liver. UDP-GlcNAc and a recombinant affinity-purified preparation of core 2 β6-N-acetylglucosaminyltransferase (EC 2.4.1.102) fused to Protein A were used to branch the core 1 structure, affording GlcNAc(β1-6)[Gal(β1-3)]GalNAc(α1-pNp in a yield of >85%. The core 2 structure was galactosylated using UDP-Gal and purified human milk β4-galactosyltransferase 1 (EC 2.4.1.38) (yield of >85%), then sialylated using CMP-Neu5Ac and purified recombinant α3-sialyltransferase 3 (EC 2.4.99.X) (yield of 87%), and finally fucosylated using GDP-Fuc and recombinant human α3-fucosyltransferase 6 (EC 2.4.1.152) produced in Pichia pastoris (yield of 100%). Overall 1.5 µmol of product was prepared. MALDI TOF mass spectra, and 1D and 2D TOCSY and ROESY 1H NMR analysis confirmed the obtained structure

Published
2018

37. NMR Spectroscopy of Sialic Acids

Author

Vliegenthart, Johannes F. G., Dorland, Lambertus, van Halbeek, Herman, Haverkamp, Johan, Alfert, M., editor, Porter, K. R., editor, Beermann, W., editor, Sitte, P., editor, Goldstein, L., editor, and Schauer, Roland, editor

Published
1982
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40. Introduction

Author

Schauer, Roland, Vliegenthart, Johannes F. G., Alfert, M., editor, Porter, K. R., editor, Beermann, W., editor, Sitte, P., editor, Goldstein, L., editor, and Schauer, Roland, editor

Published
1982
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41. Carbohydrate self-recognition mediates marine sponge cellular adhesion

Author

Haseley, Simon R., Vermeer, Henricus J., Kamerling, Johannis P., and Vliegenthart, Johannes F. G.

Subjects
Carbohydrates -- Physiological aspects, Cell adhesion -- Physiological aspects, Sponges -- Physiological aspects, Science and technology
Abstract

Sponges (Porifera), the simplest and earliest multicellular organisms, are thought to have evolved from their unicellular ancestors about 1 billion years ago by developing cell-recognition and adhesion mechanisms to discriminate against 'non-self.' Consequently, they are used as models for investigating recognition phenomena. Cellular adhesion of marine sponges is an event involving adherence of extracellular proteoglycan-like molecules, otherwise known as aggregation factors (AFs). In a calcium-independent process the AFs adhere to the cell surface, and in a calcium-dependent process they exhibit AF self-association. A mechanism which has been implied but not definitely proven to play a role in the calcium-dependent event is self-recognition of defined carbohydrate epitopes. For the red beard sponge, Microciona prolifera, two carbohydrate epitopes, a sulfated disaccharide and a pyruvylated trisaccharide, have been implicated in cellular adhesion. To investigate this phenomenon a system has been designed, by using surface plasmon resonance detection, to mimic the role of carbohydrates in cellular adhesion of M. prolifera. The results show self-recognition of the sulfated disaccharide to be a major force behind the calcium-dependent event. The interaction is not simply based on electrostatic interactions, as other sulfated carbohydrates analyzed by using this procedure did not self-associate. Furthermore, the interaction is completely eradicated on substitution of [Ca.sup.2+] ions by either [Mg.sup.2+] or [Mn.sup.2+] ions. This physiologically relevant recognition mechanism confirms the existence of true carbohydrate self-recognition, and may have significant implications for the role of carbohydrates in cellular recognition of higher organisms.

Published
2001

42. A Novel Disorder Caused by Defective Biosynthesis of N-Linked Oligosaccharides Due to Glucosidase I Deficiency

Author

De Praeter, Claudine M., Gerwig, Gerrit J., Bause, Ernst, Nuytinck, Lieve K., Vliegenthart, Johannes F. G., Breuer, Wilhelm, Kamerling, Johannis P., Espeel, Marc F., Martin, Jean-Jacques R., De Paepe, Anne M., Chan, Nora Wen Chun, Dacremont, Georges A., and Van Coster, Rudy N.

Subjects
Biosynthesis -- Physiological aspects, Oligosaccharides -- Physiological aspects, Glucosidases -- Physiological aspects, Deficiency diseases -- Genetic aspects, Biological sciences
Published
2000

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