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The annual proficiency test for the detection of animal proteins in animal feed of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy was organized by Wageningen Food Safety Research, The Netherlands. The aim of the proficiency test was to provide the participants information on the performance of the implementation of the monitoring methods for their local quality systems. A further aim was to gather information about the current practices in the application of the microscopic method. The current 2019 version of the IAG ring test for animal proteins addressed all analytical sections of the methods for microscopy and PCR as published in Regulation (EC) 51/2013 amending Annex VI of Regulation (EC) 152/2009 together with accompanying SOPs. Three of the four samples used in the proficiency test contained poultry material at the legally required technical limit (0.1% w/w; Regulation (EC) 152/2009), or fish meal at a spike level of 2% (w/w), or both. A fourth sample was left blank. A pig feed, containing 3% (w/w) of bakery by-products and a ruminant feed were used as matrix. None of the samples was labelled as fish feed. A total of 44 participants subscribed to the proficiency test animal proteins. Two participants did not submit their results and one submitted PCR results only, leaving 41 sets for microscopic evaluation. 18 sets of ruminant PCR results were submitted as well. Microscopy All participants were requested to determine the presence or absence of land animal and/or fish, to indicate the type of material found and to describe the method used to achieve these results. In total eight participants (19.5% of 41 participants) deviated from the official method by applying an incorrect number of determination cycles and/or drawing incorrect conclusions (e.g. “presence” for five particles, “absence” for ten particles). Therefore, all evaluations were based on the actual number of particles reported by all participants. Incorrect positive results (positive deviations) were expressed in a specificity score and incorrect negative results (negative deviations) were expressed in a sensitivity score. An optimal score is 1.0. The results are analysed in two ways: numbers below threshold (between 1 and 5 particles per determination cycle inclusive) have been considered positive (complying to the zero tolerance) and as alternative considered as negative (for matching the official evaluation). For all samples several participants did not detect terrestrial animal particles in the presence of fish meal (sensitivity 0.95) in contrast to the optimal result in the absence of fish meal (1.0), or erroneously reported terrestrial animal material when absent (specificity 0.93 and 0.90 in the presence or absence, respectively, of fish material). The absence of fish material in the presence of 0.1% poultry PAP resulted in a specificity score of 0.90. 37 institutes participated in both in the 2018 and 2019 studies. Based on their results an intra-laboratory reproducibility, expressed as concordance between 2018 and 2019 was calculated. Especially for the results representing specificity low concordance was found. This indicates wrong observations seemed incidental in most cases. The documentation for and training of microscopists for correct identification of particles of animal origin would deserve further attention in order to guard specificity and avoid incidental errors. Evaluation of several aspects of the application of the current microscopic methods would be beneficial for improving harmonization among the laboratories applying the microscopic method. PCR In the two samples without addition of ruminant PAP, but still containing the bakery by-products, ruminant DNA was detected by qPCR as far as analysed by the majority of the participants. The list of recognised sources such as milk and milk products, and ruminant gelatine can be extended with bakery by-products, which is important for the recycling of food by-products.

A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The organizer of the ring test was RIKILT - Wageningen UR, The Netherlands. The aim of the ring study was to provide the participants information on the performance of the local implementation of the detection method for their local quality systems. A further aim was to gather information about the application of the microscopic method. The current 2015 version of the IAG ring test for animal proteins is the first one in the IAG series of ring tests applying the full new method for microscopy as published in Regulation (EC) 51/2013 amending Annex VI of Regulation (EC) 152/2009 together with accompanying SOPs.

A ring test was organized for the microscopic determination of botanic composition in animal feed in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The organizer of the ring test was RIKILT Wageningen UR, The Netherlands. The aim of the ring study was to provide the participants information on the performance of the local implementation of the method for composition analysis of feed.

A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG – International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The aim of the ring study was to provide the participants information on the performance of the local implementation of the detection method for their local quality systems. A further aim was to gather information about the application of the microscopic method.

A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The organizer of the the ring study was to provide the participants information on the performance of the local implementation of the detection method for their local quality systems. A further aim was to gather information about the application of the microscopic method.

Methods for the detection and quantification of packaging materials are necessary for the control of the prohibition of these materials according to Regulation (EC)767/2009. A method has been developed and validated at RIKILT for bakery products, including sweet bread and raisin bread. This choice is based on the situation that this category of former food products has the highest volume for re-use as feed ingredient.

RIKILT serves as the official control laboratory for animal proteins in feeds in the Netherlands.

A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the Inernational Association for Feeding stuff Analysis, Section Feeding stuff Microscopy.

RIKILT serves as the only official control laboratory for animal proteins in feeds in the Netherlands in the framework of Directive 882/2004/EC. As National Reference Laboratory (NRL), RIKILT participated in 2 annual proficiency tests during the reporting period, in 2 additional interlaboratory studies addressing quantification issues, and in the annual meetings of EURL. In addition, RIKILT participated in a validation study for identification of bovine material by means of DNA analysis, in a workshop, and in a meeting in China. The interlaboratory study for DNA detection of bovine material showed that different protocols are comparable and that the identification of bovine material is feasible.

The International Association for Feeding stuff Analysis, section Feeding stuff Microscopy, organises annually a ring test for animal proteins for all their members. In this report the ring test for animal proteins is presented, which was organised by RIKILT in 2011 on behalf of the IAG section Feeding stuff Microscopy. A contamination level below 0.1%, i.e. 0.05% of animal proteins from terrestrial animals is also part of this ring test. The derogation to use fish meal for weaning ruminants, and the desire for further relaxation of the extended feed ban gave rise to inclusion of a contaminated fish meal, and a feel adulterated with feather meal. The main purpose of the ring test is to monitor the performance of the participating laboratories. The main part of this report presents and discusses the results in terms of sensitivity and specificity scores.

A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The organizer of the ring test was RIKILT - Institute of food safety, Wageningen University and Research Centre, The Netherlands. The aim of the ring study was to provide the participants information on the performance of the method in their laboratory. This is essential information for their individual quality systems. A further objective was to gather information about a set of analytical parameters of the microscopic method.

The detection of animal proteins in feed remains an important issue in the process of avoiding Mad Cow Disease.

The results of a proficiency test for the detection of animal proteins in animal feed by microscopy, PCR (DNA detection) and immunoassay methods are presented in this report.

Bij de ontwikkeling van een sedimenteermethode voor de bepaling van het botgehalte in separatorvlees wordt gebruik gemaakt van een vloeistof met een hoge relatieve dichtheid. Bij dit onderzoek wordt nagegaan welke vloeistof hiervoor het meest in aanmerking komt.

Door middel van histologisch-microscopisch onderzoek is nagaan of het mogelijk is blikken honde- en kattevoer op samenstelling te controleren. Daarvoor zijn 12 monsters vqn de meest gangbare honde- en kattevoerconserven via histologische technieken en diverse kleuringen onderzocht op samenstellende bestanddelen.

Een kwaliteitsbeoordeling van gehakt met behulp van histologisch, histometrisch en bacterioscopisch onderzoek. Bij de kwaliteitsbeoordeling van 46 monsters rauw gehakt, half om half, werden 3 onderzoekmethoden toegepast: 1. door middel van een histologische/microscopische analyse werd een onderzoek ingesteld naar de weefsel- en orgaansamenstelling van het gehakt ; 2. door middel van een histometrische analyse werd de verhouding collageen bindweefsel/dwarsgestreept spierweefsel vastgesteld van het gehakt ; 3. door middel van een bacterioscopische analyse werd een onderzoek ingesteld naar het aantal en de ligging van bacteriën en kiemophopingen (z.g . kiemnesten) in het gehakt.

Door middel van microscopisch onderzoek werd nagegaan of de in het kader van de Verordening (EEG) nr. 1725/79 genomen monsters voldoen aan deze verordening. Tevens werd nagegaan of de microscopisch vastgestelde afwijkingen door chemisch onderzoek konden worden bevestigd. In een periode van 4 weken werden 318 monsters gedroogde melkprodukten microscopisch onderzocht op identiteit en bijmenging. Bij 52 monsters werden afwijkingen geconstateerd. Bij 22 monsters werd op grond van de aanwezigheid van lactosekristallen en naaldkristallen de aanwezigheid verondersteld van weipoeder. Uit het chemisch onderzoek kon dit echter niet bevestigd worden. In 25 (gedenatureerde) monsters werd de aanwezigheid van zetmeel vastgesteld. Dit zetmeel is vermoedelijk afkomstig van de in het luzerne- of grasmeel verwerkte graanafvallen.

Voor Koosurnenten Kontakt werden 29 monsters ongebakken kroketten en 29 monsters gebakken kroketten afkomstig van automatieken, snackbars e.d. onderzocht. Het netto gewicht en de verhouding korst: vulling van deze produkt en werden bepaald. Verder werd er een onderzoek verricht naar de aard van het gebruikte vlees, de aanwezigheid van getextureerde soja (TVP), de samenstelling van de paneerlaag en van de vulling. Er werd een microbiologisch onderzoek ingesteld in de gebakken kroketten naar de aanwezigheid (grenswaarde) van E.coli, Salmonella en Staphylococcus aureus. Tevens werd het gehalte aan pluisvlees in het ongebakken produkt en het vetgehalte in het gebakken produkt bepaald.

Om een indruk te krijgen van de ingrediënten van in de handel verkrijgbare hamburgers werd een microscopisch onderzoek ingesteld in een aantal van deze produkten. Tevens werd de verhouding paneerlaag/vulling bepaald. 45 door de Consumentenbond gekochte monsters hamburgers werden onderzocht op netto-gewicht, de verhouding paneerlaag/vulling en op de aanwezigheid van separatorvlees (botbestanddelen), getextureerde soja (TVP), zetmeel, kruiden en de samenstelling van de paneerlaag.

Door middel van microscopisch onderzoek is nagegaan of er botbestanddelen zowel kwalitatief als kwantitatief aan te tonen zijn in boterhamworst. Verschillende monsters boterhamworst werden met behulp van diverse methoden onderzocht op botbestanddelen.

In dit literatuuronderzoek wordt ingegaan op de wettelijke eisen, de produktie en de samenstelling van separatorvlees. Verder wordt uitgebreid aandacht besteed aan de diverse analysemethoden die ontwikkeld zijn om het gebruik van separatorvlees in vleesprodukten vast te stellen.

Doel van het onderzoek was nagaan of het door middel van histologisch onderzoek mogelijk is om sojaprodukten zoals getextureerde soja (TVP), sojaconcentraat, sojaisolaat en andere plantaardige en dierlijke bestanddelen in boterhamworst aan te tonen.

Door middel van macroscopisch onderzoek nagaan of er verschillen bestaan tussen diverse soorten rijst, die op de Nederlandse markt verkrijgbaar zijn, aan de hand van de interne methoden. 38 monsters rijst werden onderling vergeleken met behulp van de interne methoden, welke beschreven zijn in de inleiding. De volgende rijstprodukten werden bekeken: padie, gedopte rijst, volwitte rijst, rondkorrelige rijst, langkorrelige rijst, breukrijst, verder op groene, krijtachtige, roodgestreepte, gespikkelde, gevlekte en gele korrels. Ook werd er gelet op aanwezigheid van kiemen, glansmiddel en bijmenging.