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BACKGROUND: Previous studies suggest that dietary vitamin C is inversely associated with gastric cancer (GC), but most of them did not consider intake of fruit and vegetables. Thus, we aimed to evaluate this association within the Stomach cancer Pooling (StoP) Project, a consortium of epidemiological studies on GC. METHODS: Fourteen case-control studies were included in the analysis (5362 cases, 11,497 controls). We estimated odds ratios (ORs) and corresponding 95% confidence intervals (CIs) for the association between dietary intake of vitamin C and GC, adjusted for relevant confounders and for intake of fruit and vegetables. The dose-response relationship was evaluated using mixed-effects logistic models with second-order fractional polynomials. RESULTS: Individuals in the highest quartile of dietary vitamin C intake had reduced odds of GC compared with those in the lowest quartile (OR: 0.64; 95% CI: 0.58, 0.72). Additional adjustment for fruit and vegetables intake led to an OR of 0.85 (95% CI: 0.73, 0.98). A significant inverse association was observed for noncardia GC, as well as for both intestinal and diffuse types of the disease. The results of the dose-response analysis showed decreasing ORs of GC up to 150-200 mg/day of vitamin C (OR: 0.54; 95% CI: 0.41, 0.71), whereas ORs for higher intakes were close to 1.0. CONCLUSIONS: The findings of our pooled study suggest that vitamin C is inversely associated with GC, with a potentially beneficial effect also for intakes above the currently recommended daily intake (90 mg for men and 75 mg for women).

BACKGROUND: Vitamin C, as an essential nutrient, has a wide range of biological effects and a variety of biological functions related to the pathogenesis of sarcopenia. Vitamin C supplementation is expected to be a novel prevention and treatment measure for sarcopenia. OBJECTIVE: To review recent research advances in the application of vitamin C in the pathogenesis and treatment of sarcopenia, and to discuss the potential role of vitamin C in the prevention and treatment of sarcopenia and possible mechanistic pathways based on published evidence. METHODS: The first author performed a computer search of PubMed, Web of Science, CNKI and other databases for relevant studies involving vitamin C in sarcopenia. The search keywords were “vitamin C, ascorbic acid, L-ascorbic acid, ascorbate, antioxidants, oxidative stress, sarcopenia, muscular atrophy, muscle weakness, muscle development, skeletal muscle regenerate, muscles, skeletal muscle” in English and Chinese, respectively. The search period was from each database inception to July 2023. After screening, 85 articles were included for further review. RESULTS AND CONCLUSION: Ensuring adequate dietary vitamin C intake or maintaining normal circulating levels of vitamin C will help to reduce age-related muscle loss and decrease the prevalence of sarcopenia. In addition, vitamin C supplementation is also useful for improving skeletal muscle mass, strength and physical function with potential synergistic effects in exercise strategies for sarcopenia. The effects of vitamin C on sarcopenia may be via the following biological mechanisms: vitamin C limits the activation of the ubiquitin-proteasome pathway mainly by inhibiting oxidative stress and inflammatory responses in skeletal muscle, thus positively regulating protein metabolic homeostasis, and may enhance mitochondrial antioxidant defenses through its antioxidant effects to maintain healthy mitochondrial function. In addition, vitamin C affects myoblast proliferation, differentiation and myotube size, mainly by increasing the expression of myogenic regulatory factors and activating protein synthesis signaling pathways, which contribute to the promotion of muscle development as well as the repair and regeneration of damaged muscle tissue. The positive effects of vitamin C in sarcopenia need to be studied in large samples and with optimized designs for important influencing factors, such as the choice of supplementation dose and duration, the design of exercise prescription when vitamin C is combined with an exercise intervention, and the assessment of the redox status of the individual. It is recommended that future studies should be conducted in older patients with sarcopenia (< 50 μmol/L) with suboptimal vitamin C status to investigate the efficacy of a combined intervention of long-term supplementation with 1 000 mg/d vitamin C (for 6 months or longer) with at least two or more types of multi-type combined exercise, with supplementation timed to take place at 1 hour after the end of the exercise, and with monitoring of markers of oxidative damage produced during the exercise such as malondialdehyde or protein hydroxyl levels were monitored. In conclusion, the optimal dose and timing of vitamin C supplementation for older adults with sarcopenia needs to be explored more, while the appropriate design of exercise prescriptions (especially the type and intensity of exercise) needs to be further determined. [ABSTRACT FROM AUTHOR]

This study aimed to determine the level of acceptability and marketability of GUOKCA (Guyabano, Okra, Camote Tops) ice cream of selected students and faculty of Francisco P. Felix Memorial National High School during the School Year 2023-2024. experimental method of research was used, utilizing survey questionnaire as the data gathering instrument. This study also used the 9 - point Hedonic rating scale for the acceptability and 5-point Likert scale for the marketability of the GUOKCA Ice Cream. There were thirty (30) Teenagers, thirty (30) Young Adults, and thirty (30) Adult respondents which included students and faculty members evaluated the acceptability and marketability of this research. The data revealed that the three groups of respondents, namely teenagers, young adults, and adult respondents evaluated the appearance, aroma, taste and texture of GUOKCA Ice Cream with 45ml, 90ml, and 120ml proportions as Very Acceptable. In addition, there were no significant differences on the evaluation of the three groups of respondents on the level of acceptability of GUOKCA Ice Cream. On the other hand, the level of marketability as evaluated by respondents obtained a rating of Very High Potential utilizing the 45ml, 90ml, and 120ml proportions in terms of supply availability, production cost, and consumer demand resulting to no significant difference on their evaluations. Furthermore, the results of physicochemical analysis from the produced GUOKCA ice cream contains 7.08 for sugar, 68.7 moisture, and less than 3* for Vitamins C with a pH Level of 5.01 at 23.5°C. Comments and suggestions were given by the three groups of respondents for further improvement of the product. [ABSTRACT FROM AUTHOR]

We demonstrated a nonenzymatic electrochemical sensor based upon vanadium-doped nickel oxide composite with graphitic carbon nitride. The g-C 3 N 4 was prepared by annealing Urea at 550 °C for 3 h in a muffle furnace. The nanocomposite was synthesized using a microwave-assisted method, followed by thermal annealing at different temperatures. The successful formation of V–NiO/g-C 3 N 4 was confirmed by X-ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy. SEM images show the layered sheets with porous structure, which depends on annealing temperature; as we increase the temperature, the porosity increases. Afterwards, the electrochemical sensing properties were verified by CV, LSV, DPV, and amperometric studies in 0.2 M KOH in the presence of Ascorbic acid and Dopamine. The experimental results show that our prepared sample has excellent electrochemical biosensing abilities towards AA and DA with LOD 1.16 μM and 0.16 μM, respectively. It also offers good selectivity, exceptional reproducibility, and long-term stability owing to the combined effect of NiO and g-C 3 N 4. Finally, the electrochemical sensing of H 2 O 2 was detected in the same environment by conducting CV and amperometric studies. Our modified electrode has proven its noble ability to sense H 2 O 2 with excellent repeatability, stability and low LOD 0.2 μM with a wide linear working range (0.5 mM–4 mM). It has the potential to be used for multi-molecule selection biosensing with excellent selectivity and sensitivity. [Display omitted] [ABSTRACT FROM AUTHOR]

Ceramics is a promising material that has been widely used as artificial bones. However, most available investigations were devoted to new material development and implant structure design, while few of studies focused on innovative hybrid implants that can act not only as implants but also as surgery tools for solving specific health issues. This paper innovatively designed and additively-manufactured a zirconia ceramic surgery tool, which can not only act as a drilling tool, but also retain itself in the drilled hole after the surgery as bone scaffold, at the meantime delivery active ingredient (Vitamin C) for fast recovery. To achieve early intervention, the proposed zirconia ceramic tool was additively manufactured with inter-connected pore structures that can benefit the recovery. The zirconia ceramic surgery tools (with 50 %, 70 % and 80 % porosity) showed the required mechanical properties to act as a drilling tool. The tool also showed drug releasing function with the different diffusion rates based on drug diffusion experiments. The zirconia ceramic tool with Vitamin C coating also showed enhanced cell adhesion and accelerated cell growth based on the osteoblast induction assessment. Based on above, the proposed zirconia ceramic tool is expected to bring new possibilities for wide applications of ceramic tools in surgeries. [ABSTRACT FROM AUTHOR]

Background: Kiwifruit, belonging to the genus Actinidia, represents a unique fruit crop characterized by its modern cultivars being genetically diverse and exhibiting remarkable variations in morphological traits and adaptability to harsh environments. However, the genetic mechanisms underlying such morphological diversity remain largely elusive. Results: We report the high-quality genomes of five Actinidia species, including Actinidia longicarpa, A. macrosperma, A. polygama, A. reticulata, and A. rufa. Through comparative genomics analyses, we identified three whole genome duplication events shared by the Actinidia genus and uncovered rapidly evolving gene families implicated in the development of characteristic kiwifruit traits, including vitamin C (VC) content and fruit hairiness. A range of structural variations were identified, potentially contributing to the phenotypic diversity in kiwifruit. Notably, phylogenomic analyses revealed 76 cis-regulatory elements within the Actinidia genus, predominantly associated with stress responses, metabolic processes, and development. Among these, five motifs did not exhibit similarity to known plant motifs, suggesting the presence of possible novel cis-regulatory elements in kiwifruit. Construction of a pan-genome encompassing the nine Actinidia species facilitated the identification of gene DTZ79_23g14810 specific to species exhibiting extraordinarily high VC content. Expression of DTZ79_23g14810 is significantly correlated with the dynamics of VC concentration, and its overexpression in the transgenic roots of kiwifruit plants resulted in increased VC content. Conclusions: Collectively, the genomes and pan-genome of diverse Actinidia species not only enhance our understanding of fruit development but also provide a valuable genomic resource for facilitating the genome-based breeding of kiwifruit. [ABSTRACT FROM AUTHOR]

AbstractThe study aimed to assess the toxic effect of cadmium (Cd) on the exocrine and endocrine functions of pancreas, the changes in pancreatic tissue after Cd withdrawal, and the protective effects of vitamin C (VC) and Nigella sativa (NS) against Cd-induced damage. Rats were assigned to: control, Cd-treated (0.5 mg/kg/d intraperitoneal [IP] injection), VC and Cd-treated (receiving 100 mg/kg/d VC orally and Cd concomitantly), NS and Cd-treated (receiving 20 mg/kg/d NS and Cd, simultaneously), and Cd withdrawal (receiving Cd for 30 d then living free for recovery for other 30 d). Blood samples were collected and post-sacrifice pancreatic specimens were processed for light and electron microscope study. Quantitative analyses of pancreatic collagen area%, pancreatic islet parameters, β cell density, and insulin immunoexpression were done. Fasting blood glucose was significantly increased in Cd-treated and Cd-withdrawal groups, while co-treatment with VC and NS caused significant reductions (p < 0.05). Cd-induced extensive degenerative changes in pancreatic acini and islets at light and ultrastructure levels. Obvious fibrosis and congestion of blood vessels were noticed. Significant reductions in pancreatic islet number, volume, and surface area and diminished beta cell count and insulin immunoexpression were observed. After withdrawal of Cd, the whole pancreatic tissue still showed a serious impact. Concomitant treatment with VC or NS obviously reduced these degenerative changes and significantly improved pancreatic islet parameters and insulin immunoexpression. VC showed a better amendment than NS, but this difference was statistically insignificant. Therefore, VC and NS could be used as prophylactic agents that lessen Cd consequences on the pancreas. [ABSTRACT FROM AUTHOR]

This study aimed to assess the prevalence of inadequate micronutrient intake and its risk factors among women one year after giving birth. 240 women of reproductive age were assessed. Micronutrient intake was assessed from two 24-hour records at three stages (3-6-12 months after childbirth). A generalized linear mixed-effect model was used to assess the factors associated with food intake. A prevalence of inadequate micronutrient intake was observed, with the exception of iron. Intake was lower among food-insecure women (zinc: β -0.13; iron: β -0.09; vitamin C: β -0.44). The postpartum period and food insecurity remained statistically associated with lower food consumption in the explanatory models tested. [ABSTRACT FROM AUTHOR]

Summary The effects of ultrasound (US) pretreatment combined with drying process on storage quality maintenance and bioactive compound accumulation of broccoli stems were investigated. Results indicated that US pretreatment (λ = 8.5 mm) delayed the decline of L* value and ascorbic acid content but inhibited the increase of a* and b* values, browning index, and microbial counts of fresh‐cut broccoli stems. Furthermore, the US application enhanced total phenolics content by activating phenylalanine ammonia‐lyase, cinnamate‐4‐hydroxylase, and 4‐coumarate‐CoA ligase in phenylpropane metabolism. Meanwhile, it also promoted γ‐aminobutyric acid (GABA) accumulation by upregulating activities of diamine oxidase, polyamine oxidase, and aminoaldehyde dehydrogenase in GABA shunt and polyamine degradation pathway, respectively, which in turn, accelerated the biosynthesis of GABA from polyamines and glutamate. In addition, compared with other three drying methods, the combined vacuum freeze drying (VFD) could further retain the sensory attributes and bioactive compounds to a maximum extent in dehydrated broccoli stems. Hence, current findings illustrated that US pretreatment combined with VFD process is beneficial for commercial quality maintenance and nutritional value enhancement, thus providing new perspectives on the comprehensive utilisation of broccoli stems. [ABSTRACT FROM AUTHOR]

Background Results Conclusion Carotenoids have various physiological functions, such as immune regulation and cancer prevention. Germination could further improve the content of carotenoids in maize seeds. In this study, yellow maize seeds (Suyu 29) were soaked and germinated with different concentrations of 24‐epibrassinolide. The changes of germination percentage, sprout length, bioactive components, antioxidant capacity and carotenoid content of the maize seeds were analyzed. Additionally, the relative expression of key genes in the carotenoid synthesis pathway was investigated.The results showed that the sprout length, germination percentage, soluble protein, free amino acids, proline, endogenous abscisic acid, vitamin C, total phenolics and carotenoids displayed a significant increasing trend compared with the control group (P < 0.05). The activity of superoxide dismutase and peroxidase increased by 55.1% and 58.5% versus the control group, and the antioxidant capacity of 2,2‐diphenyl‐1‐picrylhydrazyl, 2,2‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) and ferric reducing antioxidant power was 19.8%, 13.4% and 44.1% higher than that of the control group (P < 0.05). Compared with the control group, the expression of genes was significantly up‐regulated (P < 0.05). Under the treatment of 0.1 mg L−1 of 24‐epibrassinolide, carotenoid content reached the highest value. The carotenoids showed a positive correspondence with antioxidant enzyme activity, antioxidant capacity and total phenolics content (P < 0.05).This study showed that 0.1 mg L−1 of exogenous 24‐epibrassinolide promoted the accumulation of carotenoids and improved the antioxidant capacity and the quality of germinated maize seeds. It could provide a method for the development of germinated maize products enriched in carotenoids. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Ascorbic acid (AA), known as vitamin C, is a vital bioactive compound that plays a crucial role in several metabolic processes, including the synthesis of collagen and neurotransmitters, the removal of harmful free radicals, and the uptake of iron by cells in the human intestines. As a result, there is an absolute need for a highly selective, sensitive, and economically viable sensing platform for AA detection. Herein, we demonstrate a Pt-decorated MoS2 for efficient detection of an AA biosensor. MoS2 hollow rectangular structures were synthesized using an easy and inexpensive chemical vapor deposition approach to meet the increasing need for a reliable detection platform. The synthesized MoS2 hollow rectangular structures are characterized through field effect scanning electron microscopy (FESEM), energy-dispersive spectroscopy elemental mapping, Raman spectroscopy, and x-ray photoelectron spectroscopy. We fabricate a chemiresistive biosensor based on Pt-decorated MoS2 that measures AA with great precision and high sensitivity. The experiments were designed to evaluate the response of the Pt-decorated MoS2 biosensor in the presence and absence of AA, and selectivity was evaluated for a variety of biomolecules, and it was observed to be very selective towards AA. The Pt-MoS2 device had a higher response of 125% against 1 mM concentration of AA biomolecules, when compared to that of all other devices and 2.2 times higher than that of the pristine MoS2 device. The outcomes of this study demonstrate the efficacy of Pt-decorated MoS2 as a promising material for AA detection. This research contributes to the ongoing efforts to enhance our capabilities in monitoring and detecting AA, fostering advancements in environmental, biomedical, and industrial applications. [ABSTRACT FROM AUTHOR]

Background: Micronutrients play pivotal roles in modulating various aspects of the immune response. However, the existing literature on the association between micronutrients and autoimmune thyroiditis (AIT) remains limited and contentious. To address this gap, we conducted Mendelian randomization (MR) to investigate potential links between genetically predicted concentrations of six micronutrients (Copper (Cu), Iron (Ir), Calcium (Ca), Vitamin D (VD), Vitamin C (VC), Zinc (Zn)) and the risk of AIT. Method: Utilizing summary statistics from genome-wide association studies (GWAS) in individuals of European descent, we employed MR methodologies to elucidate the interplay between micronutrients and AIT. Three distinct MR techniques were employed: Inverse Variance Weighted (IVW), MR-Egger regression, and Weighted Median Estimator (WME). Additionally, we evaluated outcome heterogeneity using Cochran’s Q statistic and assessed pleiotropy using the MR-Egger intercept. Result: IVW analysis revealed no substantial evidence supporting a significant impact of genetically predicted micronutrient concentrations on AIT risk (Cu: OR = 0.918, P = 0.875; Ir: OR = 0.653, P = 0.264; Ca: OR = 0.964, P = 0.906; VD: OR = 0.717, P = 0.378; VC: OR = 0.986, P = 0.875; Zn: OR = 0.789, P = 0.539). Cochran’s Q test for IVW indicated no notable heterogeneity. Moreover, the MREgger intercept method suggested the presence of horizontal pleiotropy between serum VC levels and AIT (MR-Egger intercept = −0.037, p = 0.026), while no such pleiotropy was observed for other micronutrients. Conclusion: Our MR analysis does not support a causal relationship between genetically predicted concentrations of six micronutrients (Cu, Ir, Ca, VD, VC, and Zn) and the risk of AIT. [ABSTRACT FROM AUTHOR]

Background: Diabetes Mellitus Type II (DM-II) and periodontitis are interlinked chronic conditions with complex pathophysiological mechanisms. Micronutrient deficiencies have been implicated in both conditions, but the evaluation of their serum profile in individuals with DM-II and periodontitis remains underexplored. Understanding the micronutrient status in these populations could provide valuable insights into their nutritional needs and potential therapeutic interventions. Materials and Methods: Serum samples were collected from DM-II patients with periodontitis (n=50), healthy individuals with periodontitis (n=50), and healthy controls without periodontitis (n=50). Micronutrient analysis was performed using high-performance liquid chromatography (HPLC) to quantify levels of vitamins (e.g., A, C, D, E), minerals (e.g., zinc, iron, magnesium), and antioxidants (e.g., selenium, glutathione). Statistical analysis was conducted using ANOVA and post-hoc tests to compare micronutrient levels among the groups. Results: In DM-II patients with periodontitis, serum levels of vitamin C were significantly lower (p < 0.05) compared to both healthy individuals with periodontitis and healthy controls without periodontitis. Similarly, levels of vitamin D and zinc were significantly decreased (p < 0.05) in DM-II patients with periodontitis compared to healthy controls without periodontitis. However, no significant differences were observed in the levels of other micronutrients among the groups. Conclusion: This study highlights distinct alterations in the serum micronutrient profile of DM-II patients with periodontitis, particularly lower levels of vitamin C, D, and zinc. These findings suggest the importance of nutritional interventions targeting these deficiencies in managing the comorbidity of DMII and periodontitis. Further research is warranted to elucidate the mechanistic links between micronutrient status and the pathogenesis of these chronic conditions. [ABSTRACT FROM AUTHOR]

A study was conducted to investigate the potential protective effects of Bacopa monnieri extract on alcohol-induced toxicity in rats, focusing on its hypolipidemic and cardioprotective properties. For a period of 30 days, the experimental rats were orally intoxicated with alcohol (2 g/kg body weight) daily. Simultaneously, Bacopa monnieri extract was administered at a dose of 200 mg/kg body weight. The results of the study revealed that the rats subjected to alcohol-induced toxicity experienced a significant reduction in antioxidant defense systems, such as reduced glutathione (GSH) and ascorbic acid, while the activity of glutathione-S-transferase was enhanced compared to the control group. Additionally, the alcoholinduced group exhibited increased levels of triglycerides (TG) and total cholesterol (TC), as well as a significant decrease in phospholipids (PL). However, when Bacopa monnieri was supplemented along with alcohol, it significantly improved the antioxidant status and normalized the lipid profiles. These findings suggest that Bacopa monnieri possesses cardioprotective and hypolipidemic activities, which provide protection against alcohol-induced toxicity [ABSTRACT FROM AUTHOR]

Background: Latin American countries have shifted from traditional diets rich in micronutrients to a Westernized diet rich in high energy-dense foods and low in micronutrients. Objective: This study aimed to determine the prevalence of adequate micronutrient intakes in urban populations of 8 Latin American countries. Method: Micronutrient dietary intake data were collected from September 2014 to August 2015 from 9216 men and women aged 15.0 to 65.0 years living in urban populations of 8 Latin American countries. Dietary intake was collected using two 24-hour recalls on nonconsecutive days. Micronutrient adequacy of intake was calculated using the Estimated Average Requirement cut-off method. Results: In general terms, the prevalence of inadequate intake of thiamine, riboflavin, niacin, folate, cobalamin, iron, phosphorus, copper, and selenium ranged from 0.4% to 9.9%. In contrast, the prevalence of inadequacy of pyridoxine, zinc, vitamin C, and vitamin A ranged from 15.7% to 51.5%. The nutrients with a critical prevalence of inadequacy were magnesium (80.5%), calcium (85.7%), and vitamin D (98.2%). The highest prevalence of inadequate intakes was observed in the low educational level, participants with overweight/obesity, in men, and varies according to socioeconomic status. Conclusions: There is an urgent need to define direct regional actions and strategies in Latin America aimed at improving micronutrient adequacy, either through staple food fortification programs, agronomic biofortification, or food policies that facilitate economic access to micronutrient-rich foods. Plain language title: Description of the Vitamin and Mineral Consumption Status in Urban Cities of Latin America: Results of the Latin American Health and Nutrition Study. Plain language summary: Vitamins and minerals are essential for maintaining good health. However, traditional Latin American diets are changing to include foods that have a lot of sugar and fat but fewer vitamins and minerals. This study was designed to analyze the consumption of these nutrients in urban cities of 8 Latin American countries. We collected food consumption information from September 2014 to August 2015 from 9216 men and women between 15 and 65 years old using a method called 24-hour recall. To find out if participants were consuming the necessary daily amounts of vitamins and minerals, intakes were compared with the daily recommended amounts suggested by the Institutes of Medicine of the United States. We found that Latin American urban populations consume fewer vitamins and minerals than recommended. In some cases, few people do not consume the required amounts of vitamins and minerals, but in other cases, many do not. For example, few do not consume enough thiamine, riboflavin, niacin, folate, cobalamin, iron, phosphorus, copper, and selenium. However an intermediate number of people do not consume enough pyridoxine and zinc, a high number consume little vitamin C and vitamin A, and many people consume very little magnesium, calcium, and vitamin D. We found that the people who consume fewer vitamins and minerals are mostly males, overweight/obese, have lower education and varies according to their financial resources. A deficient intake of vitamins and minerals showed up in most age groups, from adolescence to adulthood. To improve vitamins and minerals intake, it is important that some foods that are frequently eaten are fortified or that the price of foods that are high in vitamins and minerals is lowered. [ABSTRACT FROM AUTHOR]

Aim. To investigate and assess the concentrations of glutathione peroxidase, superoxide dismutase, catalase, vitamin A and vitamin C in Iraqi patients infected by chronic myeloid leukemia (CML). Material and methods. Forty-eight Iraqi patients of ages ranged between (15-75) years took part in the current study. Enzymatic antioxidants (Gpx, SOD and CAT) and antioxidant vitamins (A and C) were estimated spectrophotometrically in blood sera of all patients according to age and sex variables, and then all clinical parameters were compared with healthy individuals (control groups). Results. Evaluation of oxidant-antioxidant status. Glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) enzymes concentrations recorded high significant decreasing (***p <0.0001) in blood serum of CML patients according to age and sex factors. Moreover, vitamins A and C showed a high significant decreasing (***p <0.0001) in their concentrations as measured in blood serum of CML patients with diverse ages and sexes. The GPx, SOD, CAT, Vitamin A and Vitamin C in the current study, are considered excellent biochemical markers for following the acuity of CML disease depending on age and sex variables. Conclusion. The clinical significance of oxidative stress represented by lack of enzymes and vitamins in the current study was proven. Statistically significant differences were recorded and observed between patients and control groups depending on age and sex factors. [ABSTRACT FROM AUTHOR]

Purpose: Vitamin C deficiency is associated with excess mortality in kidney transplant recipients (KTR). We aim to evaluate plasma vitamin C status at different post-transplantation moments and assess the main characteristics associated with vitamin C deficiency in KTR. Methods: Plasma vitamin C was assessed in 598 KTR at 3-, 6-, 12-, 24-, and 60-months post-transplantation, 374 late KTR with a functioning graft ≥ 1 year, and 395 potential donors. Vitamin C deficiency was defined as plasma vitamin C ≤ 28 µmol/L. Diet was assessed by a 177-item food frequency questionnaire. Data on vitamin C-containing supplements use were extracted from patient records and verified with the patients. Results: Vitamin C deficiency ranged from 46% (6-months post-transplantation) to 30% (≥ 1 year post-transplantation). At all time points, KTR had lower plasma vitamin C than potential donors (30–41 µmol/L vs 58 µmol/L). In cross-sectional analyses of the 953 KTR at their first visit ≥ 12 months after transplantation (55 ± 14 years, 62% male, eGFR 55 ± 19 mL/min/1.73 m2), the characteristics with the strongest association with vitamin C deficiency were diabetes and smoking (OR 2.67 [95% CI 1.84–3.87] and OR 1.84 [95% CI 1.16–2.91], respectively). Dietary vitamin C intake and vitamin C supplementation were associated with lower odds (OR per 100 mg/day 0.38, 95% CI 0.24–0.61 and OR 0.21, 95% CI 0.09–0.44, respectively). Conclusion: Vitamin C deficiency is frequent among KTR regardless of the time after transplantation, especially among those with diabetes and active smokers. The prevalence of vitamin C deficiency was lower among KTR with higher vitamin C intake, both dietary and supplemented. Further research is warranted to assess whether correcting this modifiable risk factor could improve survival in KTR. [ABSTRACT FROM AUTHOR]

A solution anode glow discharge (SAGD) was established as a miniaturized excitation source of atomic emission spectrometry (AES) for the detection of Cd in rhizosphere soil, carrot, and carrot leaf samples. The working conditions, such as electrolyte pH, discharge voltage, support electrolyte, solution flow rate, and discharge distance, were systematically optimized. The effect of 31 foreign ions on the Cd determination was investigated. In addition, the influences of matrix modifiers on the Cd signal intensity and interference elimination were examined in detail. The results showed that under the optimal operating parameters, the presence of Fe3+, Co2+, Pb2+, Cu2+, Cr3+, and Tl3+ ions at 30 mg/L causes severe depression effects for the detection of Cd, and adding ascorbic acid as a matrix modifier can reduce the matrix interference, improve sensitivity, and lower the limit of detection (LOD). The LOD of Cd is 4.4 μg/L, and power is below 8 W using SAGD without any matrix modifier. Compared with the solution cathode glow discharge–atomic emission spectrometry (SCGD-AES), the sensitivity is improved about 11-fold, and the power is consumed only about one-sixth. The analysis results of SAGD-AES are in agreement with the Cd-certified reference material and verification values measured by inductively coupled plasma atomic emission spectroscopy, suggesting that proposed method has high accuracy and reliability. [ABSTRACT FROM AUTHOR]

Myocardial reperfusion injury (MRI) accounts for up to 50% of the final size in acute myocardial infarction and other conditions associated with ischemia‐reperfusion. Currently, there is still no therapy to prevent MRI, but it is well known that oxidative stress has a key role in its mechanism. We previously reduced MRI in rats through a combined antioxidant therapy (CAT) of ascorbic acid, N‐acetylcysteine, and deferoxamine. This study determines the safety and pharmacokinetics of CAT in a Phase I clinical trial. Healthy subjects (n = 18) were randomized 2:1 to CAT or placebo (NaCl 0.9% i.v.). Two different doses/infusion rates of CATs were tested in a single 90‐minute intravenous infusion. Blood samples were collected at specific times for 180 minutes to measure plasma drug concentrations (ascorbic acid, N‐acetylcysteine, and deferoxamine) and oxidative stress biomarkers. Adverse events were registered during infusion and followed for 30 days. Both CAT1 and CAT2 significantly increased the CAT drug concentrations compared to placebo (P <.05). Most of the pharmacokinetic parameters were similar between CAT1 and CAT2. In total, 6 adverse events were reported, all nonserious and observed in CAT1. The ferric‐reducing ability of plasma (an antioxidant biomarker) increased in both CAT groups compared to placebo (P <.001). The CAT is safe in humans and a potential treatment for patients with acute myocardial infarction undergoing reperfusion therapy. [ABSTRACT FROM AUTHOR]

Favipiravir is a nucleoside analogue antiviral drug and inhibits the replication of many RNA viruses, especially influenza viruses. Favipiravir has also been used to treat patients with mild to moderate COVID-19 disease. However, various side effects, including neurological side effects, have been reported related to the use of favipiravir. Therefore, in this study, we aimed to investigate the possible effects of favipiravir alone or in combination with vitamin C on aged rats' brain tissue and the possible mechanisms of these effects. A total of 30 rats used in the study were randomly divided into 5 equal groups and the first group was kept as the control group. High-dose (100 mg/kg) or low-dose (20 mg/kg) favipiravir was administered alone or in combination with vitamin C (150 mg/kg) to other groups. Administration of both high and low doses of favipiravir significantly increased TBARS levels in brain tissue of aged rats. Similarly, both high and low doses of favipiravir led to significant increases in Bcl-2 and caspase-3 relative mRNA expression. However, only low dose favipiravir caused a significant increase in iNOS and IL-1β relative mRNA expression levels. Similar results were also observed in histopathological examinations. However, co-administration of vitamin C with favipiravir attenuated some of the adverse effects of favipiravir. In conclusion, in this study, it was shown that the use of favipiravir caused some adverse effects through oxidative, inflammatory and apoptotic processes in the brain tissue of aged rats, and the potential of vitamin C to alleviate these effects. [ABSTRACT FROM AUTHOR]

One of the main interests in the food industry is the preservation of food from spoilage by microorganisms or lipid oxidation. A novel alternative is the development of additives of natural origin with dual activity. In the present study, a chemically modified lysozyme (Lys) with epigallocatechin gallate (EGCG) was developed to obtain a conjugate (Lys-EGCG) with antibacterial/antioxidant activity to improve its properties and increase its application potential. The modification reaction was carried out using a free radical grafting method for the Lys modification reaction, using ascorbic acid and hydrogen peroxide as radical initiators in an aqueous medium. The synthesis of Lys-EGCG conjugate was confirmed by spectroscopic (FT-IR, 1H-RMN, and XPS) and calorimetry differential scanning (DSC) analyses. The EGCG binding to the Lys biomolecule was quantified by the Folin-Ciocalteu method; the antibacterial activity was evaluated by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MCB) against Staphylococcus aureus and Pseudomonas fluorescens; the antioxidant activity was evaluated by ABTS, DPPH, and FRAP. The spectroscopic results showed that the Lys-EGCG conjugate was successfully obtained, and the DSC analysis revealed a 20 °C increase (P < 0.05) in the denaturation temperature of Lys due to EGCG modification. The EGCG concentration in Lys-EGCG was 97.97 ± 4.7 µmol of EGCG/g of sample. The antibacterial and antioxidant activity of the Lys-EGCG conjugate was higher (P < 0.05) than pure EGCG and Lys. The chemical modification of Lys with EGCG allows for the bioconjugate with a dual function (antibacterial/antioxidant), broadening the range of Lys and EGCG applications to different areas such as food, cosmetic, and pharmaceutical industries. [ABSTRACT FROM AUTHOR]

• Late sown chickpea faces heat stress that drastically reduces grain yield in the NE plains of India. • Chickpea genotypes respond differently to late sown associated heat stress. • 'Pusa 3043′ and 'DBGC 4′ showed least yield reductions under late sown condition. • Both the genotypes mitigated heat stress by accumulating more ascorbic acid than the sensitive ones. Post rice cultivation, chickpea (Cicer arietinum L.) sowing is very often delayed due to late harvesting of preceding rice crop in the north-east plains of India. This exposes the late sown chickpea to heat stress, which substantially reduces the chickpea yield. The present study aimed to understand the most important physiological basis for genotypic response to yield differences in chickpea. Two trials, each one comprising five released cultivars and five advance breeding lines of chickpea, were conducted under timely sown and late sown conditions consecutively for two years (2018–19 & 2019–20). Three genotypes 'DBGC 1′, 'DBGC 3′ and 'Pusa 547′ were identified as high yielders under timely sown condition, whereas two other genotypes 'Pusa 3043′ and 'DBGC 4′ excelled all others, and showed least yield reductions under late sown condition. Yield under late sown condition showed significant negative and positive correlations respectively with days to flowering (50 %), maturity duration and 100 seed weight, and per day yield, yield/plant, harvest index and pods/plant. Among physiological characters, carotenoids and ascorbic acid content had very strong and positive associations with yield under late sown condition. Path coefficient analysis differentiated ascorbic acid further from other physiological attributes as it showed high and direct positive effect on yield. Significantly enhanced accumulation of ascorbic acid precisely explained better yield performance of chickpea genotypes 'Pusa 3043′ and 'DBGC 4′ under late sown condition. [ABSTRACT FROM AUTHOR]

Mono-derivatives of fullerene C60 with l-phenylalanine, o-fluoro-l-phenylalanine and p-fluoro-l-phenylalanine, as well as their hybrid nanostructures with derivatives of vitamin B12, have been synthesised. The structure of the compounds was determined by IR, UV and CD-spectroscopy. The IR spectra of the obtained compounds contain all bands characteristic of the original compounds, with the exception of the band at 1227 cm−1, which is related to the free carboxyl group in the fullerene fragment. In the UV spectra of hybrid nanostructures contain smoothed peaks in the region of 360 nm, confirming the presence of corrin ligand in the original and obtained compounds. Using the CD method, it was found that all spectra have a characteristic maximum in the region of 430 nm, confirming the presence in all hybrids nanostructures of a fragment of vitamin B12: e-COOH-Cbi(OH)2. The radiuses of nanoparticles of hybrid nanostructures, calculated by the dynamic light scattering method, range from 90 to 120 nm. The degree of association in the complexes ranges from 400 to 600 thousand molecules. It was shown that the resulting hybrid nanostructure retains catalytic activity in the autoxidation reaction of ascorbic acid, inherent in derivatives of vitamin B12. [ABSTRACT FROM AUTHOR]

Summary: Freezing is a widely used method for preserving fresh strawberries, and selecting appropriate thawing technologies is crucial for obtaining high‐quality thawed strawberries. Radio frequency technology provides a promising method owing to its expeditious uniform heating mode with substantial penetration depth. This study investigated the effects of radio frequency thawing (RFT) using electrode gaps of 12, 14, or 16 cm on the quality attributes of frozen strawberries, comparing them with refrigerator thawing (RT) and microwave thawing (MWT). The results illustrated that strawberries thawed by RFT exhibited significantly lower firmness loss and higher levels of anthocyanins and ascorbic acid compared to RT and MWT methods. Specifically, strawberries thawed with a 12‐cm electrode gap under RFT showed faster thawing rates, increased phenolic content, and enhanced antioxidant activity. Overall, radio frequency technology shows great potential in enhancing the quality of thawed strawberries. [ABSTRACT FROM AUTHOR]

Reformulation with addition of antioxidants is one potential mitigation strategy to prevent or reduce nitrosamine drug substance-related impurities (NDSRIs) in drug products. To explore whether there could be other approaches to demonstrate bioequivalence for a reformulated oral product, which typically needs in vivo bioequivalence studies to support the changes after approval, the effects of antioxidant on the in vitro permeability of BCS III model drug substances were investigated to see whether there could be any potential impact on drug absorption. Six antioxidants were screened and four (ascorbic acid, cysteine, α-tocopherol and propyl gallate) were selected based on their nitrosamine inhibition efficiencies. The study demonstrated that these four antioxidants, at the tested amounts, did not have observable impact on the in vitro permeability of the BCS III model drug substances across Caco-2 cell monolayers in the In Vitro Dissolution Absorption System (IDAS). An in vitro permeability study could be considered as part of one potential bioequivalence bridging approach for reformulated low-risk immediate release solid oral products and oral suspension products. Other factors such as the influence of antioxidants on intestinal transporter activities should be considered where appropriate. [ABSTRACT FROM AUTHOR]

The present study aims to evaluate the use of cranberry bush fruit pomace (CBFP) (Viburnum opulus L.), which has recently become popular raw material, as a substrate in the presence of a reducing agent to increase biobutanol production by Clostridium beijerinckii DSMZ 6422. For this purpose, some factors were optimized, including the pretreatment, initial concentration of CBFP (5–20%), different types of reducing agents (ascorbic acid, L-cysteine, sodium dithionite and sodium sulfite), different concentrations of sodium dithionite (2.5–15 mM), inoculum concentration (5%, 10%, and 20%), and fermentation time (24–96 h). The maximum biobutanol, total ABE, biobutanol yield, and biobutanol productivity were 9.45 g/L, 12.08 g/L, 0.21 g/g, and 0.13 g/L/h in the medium containing enzymatically hydrolyzed 10% CBFP, 10 mM sodium dithionite, and 20% inoculum at the end of 72 h, respectively. These findings demonstrate that CBFP can be considered as a sustainable, economical, and viable substrate on biobutanol production for the first time in the literature. [ABSTRACT FROM AUTHOR]

Four novel lanthanide La (III), Ce (III), Nd (III), and Dy (III) complexes with (Z)‐4‐(benzo[d]thiazol‐2‐ylamino)‐3‐((benzo[d]thiazol‐2‐ylimino)methyl)‐2H‐chromen‐2‐one (L) were prepared and characterized. The novel compounds' structural compositions were elucidated by the application of analytical and spectroscopic methodologies. From physical and chemical measurements, the general formula for the [LnLCl3]2H2O complexes (C1‐C4) was proposed, which was proven by theoretical measurements. Using cisplatin, gentamicin, ampicillin, and ascorbic acid as standards, the compound's anticancer, antibacterial, and antioxidant qualities were assessed, and activities followed the order: Ce (III)L(C2) > Nd (III)L(C3) > Dy (III)L(C4) > La (III)L(C1) > L where the Ce (III) complexes exhibited the highest activity followed by Nd (III) complexes. Molecular docking studies were performed using the MOA2022 software to identify putative binding mechanisms for the methionine adenosyl‐transferases in liver cancer (PDB ID: 5A19) and the most active site of the Bacillus subtilis receptor (PDB ID: 5e6k), where the strength of the interaction increases with the negative binding energy L ˂ [LaLCl3] ˂ [DyLCl3] ˂ [NdLCl3] ˂ [CeLCl3]. [ABSTRACT FROM AUTHOR]

Scurvy is a rare nutritional disorder caused by deficiency of ascorbic acid (vitamin C). It is often under-diagnosed in clinical settings, especially in North America where population statistics are unavailable. However, scurvy is more common than previously thought and appears to be re-emerging in children with developmental delays. Here, we review the pertinent literature and present a case of a previously healthy, 5-year-old, non-verbal boy who presented with multiple, acute, and subacute spontaneous epidural hemorrhages managed by neurosurgical intervention. He remained in hospital for 17 days and was seen in follow-up 3 weeks post-operatively having returned to his neurological baseline. Our case suggests the importance of considering scurvy in patients who have developmental delays and poor nutritional status. [ABSTRACT FROM AUTHOR]

Use of food supplements (FS) by athletes is well characterised but there is little information on 'herbal' or 'botanical' FS beyond 'natural'. This study determined, by questionnaire, whether athletes' main self‐reported reason for using FS was reflective of what was written on product labels or, when these claims were unavailable, was in accordance with the scientific literature. In 217 elite (n = 55) and amateur (n = 162) athletes living on the island of Ireland, 71% (n = 153) consumed any kind of FS, with 16% (n = 34) of the entire cohort deemed botanical consumers. 'Protein' (21%, n = 46), 'vitamin D' (17%, n = 37) and 'vitamin C' (15% n = 32) were most consumed with the top reasons for use being 'to support health', 'to prevent illness/for immunity purposes' and 'recovery'. There was generally good agreement between approved nutrition and health claims for such products and athletes' main reported reasons for use. Only the amateur athletes in our pool described using botanical supplements, with reasons for use stated as 'sleep improvement' (21%), 'recovery' (14%), 'supporting health' (12%) and 'energy' (12%), resulting in poor agreement with either approved claims or scientific evidence. Only half of amateur athletes knew if their botanical FS were third‐party tested. Athletes and practitioners require guidance to avoid consuming supplements for which there is little scientific evidence, and which may risk being contaminated/fraudulent. [ABSTRACT FROM AUTHOR]

BACKGROUND: Okra contains flavonoids and vitamin C as antioxidants and it contains polysaccharides as immunomodulators. Flavonoids regulate the inflammatory response in mice and may be related to gut microbiota. This study therefore aimed to investigate the impact of okra extract (OE) on inflammation in mice and to elucidate its underlying mechanism. METHOD: Forty male Kunming (KM) mice were categorized into four groups: the control (CON) group, the lipopolysaccharide stimulation (LPS) group, the 5 mg mL−1 OE intervention (LPS + OE) group, and the 5 mg mL−1 OE supplementation plus mixed antibiotics (LPS + OE + ABX) group. RESULTS: The results showed that, compared with the OE group, the expression of inflammatory signaling pathway genes was upregulated and gut barrier genes were inhibited in the OE + ABX group. The Fxr receptor was activated and the abundance of Akkermansia was increased after OE supplementation, whereas the effect was reversed in the OE + ABX group. Meanwhile, Fxr was correlated positively with Akkermansia. CONCLUSION: The OE supplementation alleviated the inflammatory response in mice under LPS stimulation, accompanied by changes in gut microbiota and bile acid receptors, whereas the addition of antibiotics caused a disturbance to the gut microbiota in the OE group, thus reducing the effect of OE in alleviating the inflammatory response. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

The objective of this research was to assess the impact of 2-methyl-2-nitro-3-guanidine on mammalian species, otherwise not the intended targets, followed by one-time exposure and its adverse effects. Thirty-five Sprague Dawley rats of either male or female sex, aged four weeks, and with an average weight of 80±20g, were divided into three groups randomly: control E exposed and exposed and supplemented with vitamin C, and assigned the titles C,E, and V, respectively. Each group comprised fifteen rats. Both E and V groups were further divided into subgroups: E1, E2, and E3, exposed to LD10, LD25, and LD50 doses of Dinotefuran respectively, and V1, V2, and V3, exposed in the same manner but supplemented with a fixed dose of ascorbic acid (vitamin C). Ascorbic acid was administered in aqueous form 35mg/100mL of water and provided ad libitum. Eight hours after exposure to Dinotefuran, 5ml of blood was collected under sedation through the cardiac vein. After 48 hours, two rats randomly selected from each subgroup, including the control group, were anesthetized, euthanized, and dissected. Different body tissues, and the kidney, liver, bones, and heart, were isolated and preserved in formalin solution for subsequent analysis. CBC, liver, renal, and cardiac biomarkers were evaluated. In addition, histopathology and bone characteristics of soft tissues were also conducted. Mortality and morbidity were recorded. The result showed significant disruptions in CBC and other biomarkers related to kidney, liver, and heart in a dose-dependent pattern. Although vitamin supplementation improved the overall outcome, the improvement was not statistically significant. Histopathological examination displayed changes in the exposed (E) group, with no observable improvements with vitamin supplementation. Moreover, the bone-related parameters exhibited similar trends. [ABSTRACT FROM AUTHOR]

Chili (Capsicum annuum L.) is an important functional food due to its main bioactive compound, capsaicin, and other nutritional phytochemicals. However, very few studies have been conducted to develop hybrids with a high content of nutritional phytochemicals. The present study involving six parents was conducted to identify superior hybrids with higher nutritional quality based on combining ability and heterosis following Griffing's diallel Method II Model I. A broad spectrum of genetic variation among the six parents and fifteen F1 hybrids was confirmed by analysis of variance. (H1/D)0.5 value indicated that partial dominance gene action controlled all the traits except capsaicin and total phenolic content. Based on general combining ability (GCA) results, parent P3 (PLP-2s) was the best general combiner for all the traits except K and Na, followed by the parents P6 (BU Capsicum 1), P5 (Morich-8), P4 (Chili Japan) and P1 (Red Chili). Specific combining ability (SCA), along with heterotic response, revealed that the F1 hybrid P3xP6 (PLP-2s x BU Capsicum 1) was the best hybrid, followed by the hybrids P4xP6 (Chili Japan x BU Capsicum 1) and P3xP4 (PLP-2s x Chili Japan), as they exhibited superiority for major nutritional components, such as capsaicin and ascorbic acid. Ultimately, the subsequent selection of the F1 hybrids would help develop better nutritional-quality hybrids. [ABSTRACT FROM AUTHOR]

Introduction: Xenografts of androgen-independent human DU145 prostate metastatic carcinomas implanted in nu/nu male mice have revealed a significant survival after a prooxidant anticancer treatment consisting of a combination of menadione bisulfite and sodium ascorbate (VK3:VC). Methods: Implanted samples of diaphragm carcinomas from longest survived mice from either oral, intraperitoneal (IP), or both oral and IP treatment groups were assessed with light, scanning, and transmission electron microscopy to analyze morphologic damages. Results: Compared with previous fine structure data of in vitro untreated carcinomas, the changes induced by oral, IP, and oral with IP VK3:VC treatment dismantled those xenografts with autoschizis, and necrotic atrophy was accomplished by cell's oxidative stress whose injuries were consequent to reactivated deoxyribonucleases and ribonucleases. Tumor destructions resulted from irreversible damages of nucleus components, endoplasmic reticulum, and mitochondria there. Other alterations included those of the cytoskeleton that resulted in characteristic self-excisions named " autoschizis." All these injuries lead resilient cancer cells to necrotic cell death. Conclusion: The fine structure damages caused by VK3:VC prooxidant combination in the human DU145 prostate xenografts confirmed those shown in vitro and of other cell lines with histochemistry and biomolecular investigations. These devastations incurred without damage to normal tissues; thus, our data brought support for the above combination to assist in the treatment of prostate cancers and other cancers. [ABSTRACT FROM AUTHOR]

Aim: Impairment of masticatory function in elderly patients with terminal dentition due to stage IV periodontitis (TDS4P) may lead to lower nutritional intake. The study aimed to report the dietary intake and nutrition status of elderly patients with TDS4P and compare them with those of the elderly Chinese population and the Chinese Dietary Reference Intakes (DRIs). Materials and Methods: Fifty‐one consecutive subjects (≥55 years old) with TDS4P were enrolled. Average dietary intake was evaluated based on a 3‐day 24‐h dietary recall (24HR) and food frequency questionnaire (FFQ). The daily intake of fresh vegetables and fruits, dietary energy as well as macro and micronutrients were calculated and compared with matched national data and the Chinese DRIs. Nutritional status was assessed by Short‐Form Mini‐Nutritional assessment. Results: Of the subjects, 19.6% (95% CI: 7.2%–28.1%) were at risk of malnutrition. The mean daily energy intake was 1517.4 kcal (95% CI: 1400.5–1634.3) for males and 1110.7 kcal (95% CI: 1001.5–1219.9) for females, which were very low compared with both the national data and the DRIs. Females derived a higher percentage of energy from fat. The mean daily intake of vegetables was 151.4 g (95% CI: 128.1–174.8) by FFQ and 130.9 g (95% CI: 104.6–157.3) by 24HR. Both results were significantly lower than the national reports (95% CI: 310.3–340.1) and the DRIs (300–450 g). Insufficient micronutrient intake, especially vitamins A, C and E, was also found. Conclusions: Elderly subjects with TDS4P had a lower daily energy intake, vegetable and fruit consumption and essential macro and micronutrient intake. More studies are needed to clarify the impact of periodontitis and tooth loss/replacement on nutrition and healthy ageing. [ABSTRACT FROM AUTHOR]

Salinity, a significant abiotic stressor, imperils vegetable growth, yield, and quality. Moreover, elevated salinity levels, driven by climate change, jeopardize vegetable nutritional quality. In particular, protected cultivation systems, responsible for 60% of the global vegetable industry's economic value, encounter notable challenges in managing salinity due to water runoff and drainage mechanism limitations. Therefore, it is crucial to understand the intricate mechanisms that control salinity and use this knowledge to improve plant tolerance to these conditions. In this study, we explore strategies to effectively mitigate the detrimental impacts of salinity on vegetable crops cultivated within protected environments. Additionally, we investigate the benefits of controlled moderate salinity adjustments in protected cultivation to enhance their nutritional content. Moderate salinity or nutrient solution increases typically raise total soluble solids, sugar, vitamin C, phenols, lycopene, and antioxidants in most fruit vegetables. Though generally applicable to leafy vegetables, exceptions like lettuce and wild rocket may show inconsistencies, potentially reducing some quality traits. Interdisciplinary approaches are essential to developing sustainable solutions for managing salinity in protected cultivation systems, thereby ensuring the resilience of vegetable production in the face of changing environmental conditions. Given the impracticality of desalinating all areas, future research should also investigate synergies between moderate salinity stress, cultivars used, and environmental factors from physiological and molecular perspectives to enhance vegetable nutritional quality. [ABSTRACT FROM AUTHOR]

Background: The exact pathogenesis of melasma is not yet known, and its treatment remains challenging. Mesotherapy with tranexamic acid (TXA) and vitamin C was both reported to have certain effects on melasma. In spite of that several articles have compared the efficacy and safety of the two drugs on melasma, most of them were clinical study with small sample size. Aims: To evaluate the efficacy and safety of mesotherapy with TXA versus vitamin C in treating melasma through meta‐analysis and systemic review. Methods: The authors searched PubMed, Web of Science, Springer, and ScienceDirect for studies that compared mesotherapy with TXA versus vitamin C as a treatment for melasma. Primary outcomes were change in melasma area and severity index (MASI) before and after the treatment. Results: Finally, five studies with a total of 127 patients were included in the systematic review. There was no statistic difference in the change in MASI score between the TXA and vitamin C groups (mean difference, 0.16; 95% CI, −0.79 to 1.11). Conclusions: Mesotherapy with both TXA and vitamin C is safe and effective in the treatment of melasma. [ABSTRACT FROM AUTHOR]

To improve the performance of biodegradable materials and endow them with antimicrobial properties, herein, pyridine-3,5-dicarboxylic acid was introduced as a third monomer into the molecular chain of polybutylene succinate (PBS) via copolymerization, followed by introduction of pyridine quaternary ammonium into the PBS branched chain through nucleophilic substitution to afford antimicrobial PBS-BD, which was then comingled with polylactic acid (PLA) to prepare PBS-BD/PLA composites. X-ray diffraction analysis revealed that the introduction of pyridine-3,5-dicarboxylic acid caused a small shift in the diffraction peaks of PBS and a decrease in crystallinity, which was further decreased upon blending with PLA. PBS-BD and PLA were bound together by intermolecular forces. When the PBS-BD/PLA ratio was 8:2, the melting points of the two phases were the closest, the compatibility was the best, and the thermal and antimicrobial properties were optimal. The antimicrobial properties of the composites were gradually enhanced with increasing PBS-BD content, reaching optimal values for application as antimicrobial materials. The composites were degraded in soil supernatant for 6 months at a rate of 31.52%. After 42 d of enzymatic degradation, the degradation rate reached 23.24%. The PBS-BD/PLA composites promoted the growth of green vegetables to a certain extent and enhanced their nutritional value, reaching the highest chlorophyll content and vitamin C content after 45 d of growth in the presence of the composites. [ABSTRACT FROM AUTHOR]

Citrus juices are rich sources of antioxidants, including vitamin C, but are prone to oxidation during storage. We investigated the application of gallic acid (GA), an environmentally friendly reducing agent, as active packaging to mitigate this issue. Orange juice was packaged in airtight glass containers both with and without GA‐based oxygen scavenger (GA‐OS) label and stored at 20°C and 4°C. During the storage, we monitored oxygen levels in the headspace and dissolved oxygen concentration in the orange juice, vitamin C content and browning. In the presence of GA‐OS, oxygen levels in the headspace were reduced by half in under 1 h at 21°C and within 2 h at 4°C. Dissolved oxygen concentration decreased 50% after 8 h at 21°C and after 1.2 days at 4°C. In the absence of GA‐OS, vitamin C degraded completely within 30 days at 21°C and 45 days at 4°C. In contrast, GA‐OS nearly fully preserved vitamin C for 105 days at both temperatures. Browning, quantified by delta E, escalated in GA‐OS‐absent samples to 16.79 ± 0.16 at 21°C to 12.19 ± 0.10 at 4°C, whereas GA‐OS samples exhibited no significant browning. Our results underscore GA‐OS's efficacy in preserving the quality of liquid foodstuffs, exemplified by orange juice, suggesting its broad range applicability as active packaging. [ABSTRACT FROM AUTHOR]

Polygonum minus Huds. has massive potential to be commercialized following many current discoveries of its medicinal properties. We investigated the growth and physiology changes of P. minus under different shading and treatment durations. The effects of four shading levels: 0% (T1), 30% (T2), 50% (T3) and 70% (T4) and two treatment durations of 10 weeks and 20 weeks were studied. Significant effects of shading levels and treatment durations were observed on the transpiration rate (E), leaf resistance, pigment content in leaves (Chl a, Chl b and Car), carbohydrate, ascorbic acid, total anthocyanin, phenolic and flavonoid contents. Enzyme activity of superoxide dismutase and ascorbate peroxidase were also significantly affected by shading levels and treatment durations. The highest antioxidant activities were recorded under T4 of both treatment durations along with the high content of Chl a, Chl b and Car. The leaf, stem and root extracts of P. minus were also subjected to HPLC analysis for quantification of selected flavonoid compounds. It was observed that longer treatment duration of 20 weeks reduced the amount of flavonoid. [ABSTRACT FROM AUTHOR]

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Herein, we investigated the effects of immunostimulants on the growth, immune responses, and post-infection survival of hybrid sturgeons (Huso huso♀ × Acipenser ruthenus♂). Therefore, we examined the effects of dietary supplementation of β-glucan, vitamin C, and Radix Bupleuri (Chaihu) extract (RBE) at various concentrations (0.2, 0.4, and 0.6% incorporated through feed) on the growth rate and non-specific immune responses of hybrid sturgeon. When supplemented individually, these immunostimulants significantly reduced the feed conversion ratio while promoting weight gain, with the vitamin C-administered groups showing the most pronounced differences. Further, we observed that dietary β-glucan, vitamin C, and RBE, when fed at 0.4 and 0.6% concentrations, significantly enhanced the rates of superoxide anion production and phagocytosis by head kidney leukocytes. Moreover, vitamin C and RBE supplementation at 0.4 and 0.6% and β-glucan supplementation at 0.6% concentration significantly increased the serum lysozyme activity. Further, vitamin C and β-glucan supplementation at 0.6% and RBE supplementation at 0.4 and 0.6% concentrations showed the highest survival rates after Aeromonas hydrophila infection. In conclusion, our findings highlight that the dietary supplementation of β-glucan, vitamin C, or RBE in appropriate concentrations can significantly enhance superoxide anion production and phagocytic activity of leukocytes, serum lysozyme activity, and the survival of hybrid sturgeons after A. hydrophila infection. Our results suggest that incorporating immunostimulants such as Chinese medicinal herbs at optimal levels can effectively boost the growth, immunity, and disease resistance of hybrid sturgeons in aquaculture. These results provide valuable insights for future studies on the inclusion of immunostimulants in aquaculture feed.Highlights: Adding immunostimulants β-glucan, vitamin C, and Radix Bupleuri (Chaihu) extract (RBE) to hybrid sturgeon food can significantly improve food conversion and weight gain rates, with the addition of vitamin C being the most significant. β-glucan, vitamin C, and RBE influence the innate immune system of hybrid sturgeon. Adding immunostimulants β-glucan, vitamin C, and RBE to hybrid sturgeon food can prevent A. hydrophila infection. β-glucan, vitamin C, and RBE are potential immunostimulants for hybrid sturgeon breeding. [ABSTRACT FROM AUTHOR]

Copyright of Baghdad Science Journal is the property of Republic of Iraq Ministry of Higher Education & Scientific Research (MOHESR) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

AbstractBackground and AimsMethodsResultsConclusionsInformation on effective bowel preparation (BP) methods for patients with constipation is limited. We recently reported the efficacy of 1 L polyethylene glycol plus ascorbic acid (PEG-Asc) combined with senna for BP; however, this regimen was insufficient in patients with constipation. We hypothesized that the addition of linaclotide, which is approved for the treatment of chronic constipation, to 1 L PEG-Asc would yield results superior to those of senna in patients with constipation.This was a retrospective, single-center study that included outpatients with constipation who underwent BP prior to colonoscopy between March and December 2019 (receiving 1 L PEG-Asc with 24 mg senna) and between January and October 2020 (receiving 1 L PEG-Asc with 500 mg linaclotide).A total of 543 patients with constipation were included, of whom 269 received linaclotide and 274 received senna. The rate of inadequate BP was significantly lower (11% vs 20%, p < 0.01) and the adenoma detection rate was significantly higher (54% vs 45%, p = 0.04) in the linaclotide group than in the senna group. Multivariate analysis revealed that the linaclotide regimen significantly reduced the risk of inadequate BP (odds ratio = 0.36, 95% confidence interval = 0.21–0.60, p < 0.01).The linaclotide regimen significantly increased BP efficacy and the adenoma detection rate compared with the senna regimen without reducing tolerability and is therefore a promising new option for BP in patients with constipation. [ABSTRACT FROM AUTHOR]

Investigating the different chemical species of soluble iron in food digests provides more relevant information on the nutritional potential of an iron‐rich food. The objective of this study was to assess the bioaccessibility and speciation of iron from various aqueous extracts of Moringa (Moringa oleifera) leaves. Aqueous extracts were prepared from fresh and dried Moringa leaves using infusion and decoction methods. Spectrophotometric assays were performed to quantify inhibitors and enhancers of iron absorption in the extracts, bioaccessible iron, and its different chemical species. The highest contents of inhibitors (239.43 mg/L for polyphenols and 2.92 mg/L for phytates) and enhancers of iron absorption (1.58 mmol/L for carotenoids and 488.00 mg/L for ascorbic acid) were found in the 5‐minute decoction extract of fresh leaves, and the lowest in all infusion extracts (27.34 mg/L for polyphenols, 0.50 mg/L for phytates, 0.15 mmol/L for carotenoids, and 86.00 mg/L for ascorbic acid). The percentages of bioaccessible iron were higher for decoction extracts (42.57–52.70%) compared to infusion extracts (33.89–36.44%). Ferrous iron was the dominant inorganic species of bioaccessible iron and was more concentrated in the digests of decoction extracts (1.32–4.85 mg/L). The highest content of organic iron (5.33 mg/L) was found in the digest of the 8‐minute decoction extract of dried leaves. Drinking decoction extracts of fresh and dried Moringa leaves could be recommended to alleviate iron deficiency in vulnerable groups of the population living in areas where this plant can grow. [ABSTRACT FROM AUTHOR]

The rising demand for portable energy conversion devices has spurred the advancement of direct liquid fuel cells (DLFCs) employing fuels such as alcohol, ammonia, hydrazine, and vitamin C. In these devices, various precious metal platforms have been explored to increase the de-electronation kinetics and reduce catalyst poisoning, but with substantial cost implications. We demonstrate the crucial role of ligands in non-precious organometallic complexes in influencing the de-electronation kinetics of fuel molecules through a unique substrate–ligand synergistic interaction. This unique chemistry imparts electron deficiency at the catalytic metal center while simultaneously populating the ligand with an extensive proton charge assembly. This distinct substrate–ligand interaction enhances the DLFC performance by coulombically dragging the substrate with a distinct amplification in its de-electronation kinetics. By integrating this approach with a ferricyanide/ferrocyanide half-cell reaction, a precious metal-free vitamin C fuel cell is developed, which is capable of generating an open circuit voltage of ∼950 mV, a peak power density of ∼97 mW cm−2 at a peak current density of ∼215 mA cm−2 with the performance metrics nearly 1.7 times higher than a precious metal based DLFC. This highlights the potential of the substrate–ligand synergy in the design of efficient molecular catalysts for energy conversion applications. [ABSTRACT FROM AUTHOR]

BACKGROUND RESULTS CONCLUSION The co‐application of biochar and wood vinegar has demonstrated the potential to enhance premium crop production. The present study reveals the effects of co‐applying rice husk biochar and wood vinegar (both foliar and soil application) on soil properties and the growth of Chinese cabbage (Brassica chinensis L.) in a two‐season pot experiment.The soil pH, electrical conductivity and dissolved organic carbon contents in combination treatments of wood vinegar and biochar were increased more when wood vinegar was applied to soils rather than to leaves, and the parameters were observed to surpass those for chemical fertilizer treatments. The biomass of Chinese cabbage shoots was significantly increased by 60.8‐ and 27.3‐fold in the combined treatments compared to the control when 1% wood vinegar was sprayed to the leaves (WF1) in 2022 and 2023, respectively. Higher contents of vitamin C, soluble protein and soluble sugar were also observed in the combined wood vinegar and biochar treatments compared to chemical fertilizer treatments and the control; for example, the vitamin C content of plant shoot in WF1 was 21.3 times that of the control. The yield and quality of plants were decreased across all treatments in 2023 compared to 2022 but the combination treatments still displayed superiority.The co‐application of wood vinegar and biochar enhances the growth and improve the quality of Chinese cabbage through improving the soil properties and plant photosynthesis. Moreover, the foliage application of wood vinegar is more preferable compared to soil application. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]