101 results on '"Viola-Magni, M. P."'
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2. TEM cytochemical study of the localization of phospholipids in interphase chromatin in rat hepatocytes
- Author
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Fraschini, A., Albi, E., Gahan, P. B., and Viola-Magni, M. P.
- Published
- 1992
- Full Text
- View/download PDF
3. Third International Congress of Histochemistry and Cytochemistry
- Author
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Abe, Muneaki, Akamatsu, Masasuke, Matsumoto, Takaharu, Ohuchi, Nobuo, Masuya, Tomichi, Abe, T., Nakashio, K., Kazama, M., Matsuda, M., Adams, C. W. M., Virág, S., Morgan, R. S., Orton, C. C., Adams, Jean R., Wilcox, Theodore A., Akert, Konrad, Alberti, Rachele, Allen, Robert C., Moore, Dorothy J., Tyndall, Richard L., Anderson, Paul J., Song, Sun K., Angelakos, E. T., King, M. P., Appleton, Timothy C., Arstila, Antti U., Trump, Benjamin F., Lauria, A., Bahr, Gunter F., Wied, George L., Bartels, Peter H., Bajusz, E., Balogh, Kåroly, Barer, R., Barka, Tibor, Barnard, Eric A., Komender, Janusz, Wieckowski, Jan, Barrnett, R. J., Barron, K. D., Koeppen, A. H., Bernsohn, J., Bélanger, Leonard F., Beltrami, Carlo Alberto, Björklund, A., Ehinger, B., Falck, B., Boadle, Margaret C., Bloom, Floyd E., Bona, C., Bradshaw, M., Monus, L., Stroman, S., Budd, G. C., Salpeter, M. M., Bukhonova, A. I., Burnasheva, S. A., Jurzina, G. A., Burt, Alvin M., Chang, Jeffrey P., Schatzki, Peter F., Saito, Takuma, Chavin, Walter, Chyle, M., Korych, B., Lojda, Zdenek, Patocka, F., Cohn, Z. A., Conning, D. M., Coutinho, Hélio B., Rocha, Jácia T., Jales, Benjamin F., Cunningham, Lew, Heitsch, Richard, Daneholt, B., Edström, J.-E., Danilova, L. V., Rokhlenko, K. D., Dauwalder, M., Whaley, W. G., Kephart, J. E., Deitch, Arline D., Sawicki, Stanley G., Godman, Gabriel C., Della Corte, Francesco, Desmet, V. J., Bullens, A.-M., De Groote, J., Heirwegh, K. P. M., Diculescu, I., Onicescu, Doina, Szegly, G., Doane, Winifred W., Donskikh, N. V., Novikov, V. D., Subbotin, M. Ya., Tsirelnikov, N. I., Doolin, Paul F., Birge, Wesley J., Droz, Bernard, Bergeron, M., Drukker, J., Duarte-Escalante, Ovidio, Dubowitz, Victor, Dupraw, E. J., Eckner, Friedrich A. O., Blackstone, Eugene H., Moulder, Peter V., Ehrlich, M. P., Ellis, Stanley, McDonald, J. Ken, Callahan, P. X., Enesco, Hildegard E., Engel, W. King, Epifanova, O. I., Lomakina, L. Ya., Terskikh, V. V., Ericsson, Jan L. E., Jakobsson, Sten, Eristawi, K. D., Sharashidze, L. K., Sturua, N. S., Fabris, Guidalberto, Mariuzzi, Gianmario, Nenci, Italo, Fahimi, H. Dariush, Karnovsky, Morris J., Fand, Sally B., Farquhar, Marilyn G., Felgenhauer, K., Glenner, G. G., Stammler, A., Filkuka, J., Svejda, J., Áubrechtova, V., Filotto, U., Fischbein, J. W., Rutenburg, Alexander M., Fisher, Donald B., Forni, Alessandra, Nencioni, Torquato, Ballare’, Gianfranco, Fotin, Ludmila, Popescu, Maria, Frankfurt, O. S., Friend, Daniel S., Fuchs, B. B., Arutyunov, V. D., Shnaper, A. L., Gabunia, U. A., Shiukashvili, N. N., Gahan, P. B., Anker, P., Stroun, M., McLean, Jean, Galjaard, H., Bootsma, D., Ganina, K. P., Garcia, Alfredo Mariano, Garrett, J. R., Gepts, W., Gregoire, F., Ooms, H., Gerzeli, Giuseppe, Giacobini, Ezio, Hovmark, Stefan, Gilkerson, Seth W., Glick, David, Godlewski, H. G., Huszczuk, A., Penar, Barbara, Goldfischer, Sidney, Sternlieb, Irmin, Goldstone, A., Szabo, E., Koenig, Harold, Gornak, K. A., Goslar, H. G., Grigoriadis, P., Jaeger, K. H., Gössner, W., Benoit, H., Gracheva, Nina D., Grillo, T. Adesanya Ige, Gropp, A., Gross, U. M., Gueft, Boris, Guha, S., Fouquet, J. P., Håkanson, R., Owman, Ch., Sporrong, B., Hale, A. J., Marshall, D. J., Switsur, V. R., Hanker, Jacob S., Zenker, Nicolas, Morizono, Yoshihisa, Deb, Chandicharan, Seligman, Arnold M., Hardonk, M. J., Elema, J. D., Koudstaal, Joh., Hoedemaeker, Ph. J., Hayashi, Masando, Heller, A., Hernández, F., Martinez De Morentin, J., Herrmann, Hans-Jürgen, Hershey, Falls B., Hess, H. H., Pope, A., Bass, N. H., Hewitt, J. M., Guigon, M., Bolubasz, J., Himes, M. H., Burdick, C., Hirai, Kei-Ichi, Takamatsu, Hideo, Hirose, Shunta, Hirsch, Hilde E., Hodges, Donald R., Costoff, Allen, McShan, W. H., Holtzman, Eric, Holubar, K., Tappeiner, J., Wolff, K., Hopsu-Havu, Väinö K., Hosannah, Yvonne, Blackwood, Carlton E., Mandl, Ines, Hoskins, Godfrey C., Hugon, J. S., Borgers, M., Hurwitz, Lawrence S., Rubinstein, Lucien J., Ibrahim, M. Z. M., Imura, Shin-Ichi, Takeda, Masanori, Jacobsen, N. O., Jørgensen, P. Leth, Jarrett, A., Joandrea-Casian, Claudia, Prundeanu, Cornelia, Johnson, Anne B., Johnson, Waine C., Alkek, David S., Jongkind, J. F., Swaab, D. F., Jos, J., Junqueira, L. C., Toledo, A. M. Souza, Kaiser, Hans E., Kakari, Sophia, Kalina, Moshe, Bubis, Jose. J., Kamentsky, L. A., Kasten, Frederick H., Kiefer, Gunter G., Sandritter, W., Killander, D., Rigler, R., Kishino, Yasuo, Kobayashi, H., Urano, A., Yokoyama, K., Koelle, George B., Hughes, Charles, Korhonen, L. Kalevi, Kramer, M. F., Poort, C., Kreutzberg, Georg W., Künzel, Erich, Tanyolac, Attila, Labella, Frank S., Langley, O. K., Lanza, Giovanni B., Lappano-Colletta, Eleanor Rita, Leblond, C. P., Merzel, J., Cheng, Hazel, Nadler, N. J., Herscovics, Annette A., Lederer, B., Mittermayer, C., Lee, Sin Hang, Torack, Richard M., Lehrer, Gerard M., Bornstein, Murray B., Katzman, Robert, Leites, F. L., Tendetnik, Ju. J., Ruchadse, E. S., Rjadneva, O. E., Leppi, T. John, Kinnison, Patricia A., Gaffney, Susan P., Lev, Robert, Gerard, Andre, De Graef, Jacques, Jerzy Glass, George B., Lhotka, J. F., Jr., Anderson, J. W., Liber, Amour F., Lillie, R. D., Pizzolato, Philip, Lindner, J., Grasedyck, K., Johannes, G., Freytag, G., Lipchina, L. P., Aksyutina, M. S., Yablonovskaya, L. Ya., Lipetz, Jacques, Liu, J. C., Roizin, L., Lodin, Z., Kage, M., Hartman, J., Srajer, J., Fric, Premysl, Long, Margaret E., Sommers, Sheldon C., McGarry, E. E., Nayak, R., Birch, E., Beck, J. C., McMillan, Paul J., Adeoye, Christopher ’Seinde, Macovschi, O., Maeda, Ryuei, Ihara, Nobuo, Kanazawa, Kokichi, Maeir, David M., Wagner, Lenore, Maggi, Viviane, Franks, L. M., Livingston, D. C., Coombs, M. M., Wilson, Patricia D., Carbonell, A. W., Malyuk, V. I., Romanini, Manfredi, Gabriella, Maria, Fraschini, Annunzia, Porcelli, Franca, Manocha, Sohan L., Shantha, Totada R., Bourne, Geoffrey H., Marques, Dante, Bastos, A. L., Baptista, A. M., Vigario, J. D., Nunes, J. M., Terrinha, A. M., Silva, J. A. F., Masurovsky, E. B., Benitez, H. H., Kim, S-U., Murray, M. R., Matschinsky, F. M., Rutherford, C. L., Guerra, L., Matturri, L., Curri, S., Melnick, P. J., Mendelsohn, M. L., Conway, T. J., Perry, B., Prewitt, J. M. S., Mercado, Teresa I., Miksche, Jerome P., Misch, Donald W., Misch, Margaret S., Mitchell, J. P., Mizuhira, Vinci, Uchida, Kazuko, Amakawa, Takanori, Shindo, Hideo, Totsu, Junichi, Suesada, Ikuo, Mizutani, Akira, Monis, Benito, Candiotti, Alberto, Mori, G., Ingrami, A., Morikawa, Shigeru, Yamamura, Masao, Harada, Takayuki, Hamashima, Yoshihiro, Mullaney, P. F., Dean, P. N., Van Dilla, M. A., Müller, Gerhard, Müller, Otfried, Nakane, Paul K., Neurath, Peter W., Curtis, Zay B., Selles, William, Vetter, Henri G., Norgren, P. E., Novikoff, Alex B., O’Brien, Regina, Ohringer, Philip, Spitaleri, Vincent, Olszewska, M. J., Gabara, B., Konopska, L., Parfanovich, M. I., Sokolov, N. N., Berezina, O. N., Fadeeva, L. L., Pauly, John E., Scheving, Lawrence E., Pearse, A. G. E., Pearson, Bjarne, Bennett, William, Esterly, John R., Standen, Alfred C., Pelc, S. R., Viola-Magni, M. P., Penttilä, Antti, Perez, Vernon J., Moore, Blake W., Peters, Theodore, Jr., Danzi, J. Thomas, Ashley, Charles A., Petrova, A. S., Probatova, N. A., Philippens, Karel, Pilgrim, C., Pollock, B. M., Presnov, M. A., Preston, Kendall, Jr., Preto Parvis, V., Cisotti, F., Prewitt, Judith M. S., Mayall, Brian H., Mendelsohn, Mortimer L., Pryse-Davis, John, Sandler, Merton, Quay, W. B., Raikhlin, N. T., Rasch, Ellen M., Riecken, E. O., Goebell, H., Bode, C., Rigatuso, Joseph L., Ringertz, N. R., Bolund, L., Ritter, Carl, Thorell, Bo, Rizzotti, M., Aureli, G., Balduini, C., Castellani, A. A., Rosenbaum, Robert M., Rosene, Gordon L., Rossi, Ferdinando, Rost, F. W. D., Roth, Daniel, Ruch, Fritz, Ruddle, Frank H., Lubs, Herbert A., Ledley, Robert S., Shows, Thomas B., Roderick, Thomas H., Kim, H., Brodie, E., Rosales, C. L., Sadauskas, P., Luksys, L., Dabkevcius, V., Sakharova, A. V., Sakharov, D. A., Samosudova, N. V., Ogieveckaja, M. M., Kalamkarova, M. B., Sandler, Maurice, Santti, R. S., Sasaki, Mitsuo, Takeuchi, Tadao, Satir, P., Schauer, Alfred, Scher, Stanley, Haley, Patricia L., Schiebler, T. H., Schiemer, Hans-Georg, Schlüns, Jürgen, Schuster, F. L., Hershenov, B., Scott, J. E., Scott, T. Gilbert, Seno, Satimaru, Yokomura, Ei-Ichi, Itoh, Nobutaka, Yamamoto, Michio, Shungskaya, V. E., Enenko, S. O., Lukyanova, L. D., Sarch, E. N., Shuter, Eli, Jungalwala, Firoze, Robins, Eli, Sierakowska, Halina, Silverman, L., Simard, A., Daoust, R., Smith, Edgar E., Smith, R. E., Fishman, William H., Henzl, Milan, Sobel, Harold J., Avrin, Erna, Sorokin, Helen P., Sorokin, Sergei, Squier, C. A., Waterhouse, J. P., Steinbach, Günter, Steplewski, Zenon, Stitnimankarn, Tinrat, Stoward, Peter J., Straus, W., Stumpf, Walter E., Roth, Lloyd J., Sylvén, B., Kanamura, Shinsuke, Templeton, McCormick, Tewari, H. B., Tyagi, H. R., Thalmann, R., Glismann, L., Thomas, E., Tice, Lois W., Tixier-Vidal, A., Törö, I., Bacsy, E., Vadasz, Gy., Rappay, Gy., Tsou, K. C., Chang, Mildred Y., Matsukawa, S., Goodwin, Cleon, Lynm, Dwo, Seamond, Bette, Van Der Ploeg, M., Van Duijn, P., Coulter, J. R., Pascoe, E., Van Fleet, D. S., VanHouten, Wiecher H., Vecher, A. S., Masko, A. A., Predkel, K. I., Reshetnikov, V. N., Tchaika, M. T., Velican, C., Velican, Doina, Vendrely, C., Lageron, A., Tournier, P., Vialli, Maffo, Prenna, Giovanni, Vilter, Voldemar, Vittek, Josef, Vogt, Arnold, Vollrath, L., Von Mayersbach, Heinz, Vorbrodt, Andrzej, Wächtler, Klaus, Wakabayashi, Katsumi, Tamaoki, Bun-Ichi, Wald, Niel, Ranshaw, Russell, Weller, Roy O., Welsch, Ulrich, Werner, Gottfried, Williams, Vick, Morriss, Fran, Willighagen, R. G. J., Wilson, Barry W., Wohlrab, Frank, Wolf, Paul L., Horwitz, Jerome P., Freisler, Josef V., Von Der Muehll, Elisabeth, Vazquez, Janice, Wolman, Moshe, Yamada, Masaoki, Iwata, Sunao, Yamaguchi, Hisao, Yataganas, X., Gahrton, G., Young, Ian T., Zaccheo, D., Grossi, C. E., Genta, V., Riva, A., Zacks, S. I., Sheff, M. F., Zamfirescu-Gheorghiu, M., Serban, M., Vladescu, C., Chirulescu, Z., Marcus, N., Zelenin, A. V., Kirianova, E. A., Stepanova, N. G., Zeuthen, Erik, Zimmermann, Horst, Zugibe, Frederick T., Abrahamson, Dean E., Anderson, Norman G., Caspersson, Törbjorn, Cornell, Richard, Dougherty, William, Jirasek, J. E., Jonsson, Gösta, Leske, Regina, Moyer, Frank H., Schneider, Walter C., Siegel, Howard I., Sternberger, Ludwig, Osserman, Elliott F., Weinstock, A., and Rosenbaum, Robert M.
- Published
- 1968
- Full Text
- View/download PDF
4. Metabolic DNA in the hepatocyte nuclei in newborn rats
- Author
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Bibbiani, C. and Viola-Magni, M. P.
- Published
- 1975
- Full Text
- View/download PDF
5. Changes of DNA content per nucleus in hepatocytes of rat during the first days of postnatal life
- Author
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Bibbiani, C., Tongiani, R., and Viola-Magni, M. P.
- Published
- 1973
- Full Text
- View/download PDF
6. Third International Congress of Histochemistry and Cytochemistry
- Author
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Muneaki, Abe, primary, Akamatsu, Masasuke, additional, Matsumoto, Takaharu, additional, Ohuchi, Nobuo, additional, Masuya, Tomichi, additional, Abe, T., additional, Nakashio, K., additional, Kazama, M., additional, Matsuda, M., additional, Adams, C. W. M., additional, Virág, S., additional, Morgan, R. S., additional, Orton, C. C., additional, Adams, Jean R., additional, Wilcox, Theodore A., additional, Akert, Konrad, additional, Alberti, Rachele, additional, Allen, Robert C., additional, Moore, Dorothy J., additional, Tyndall, Richard L., additional, Anderson, Paul J., additional, Song, Sun K., additional, Angelakos, E. T., additional, King, M. P., additional, Appleton, Timothy C., additional, Arstila, Antti U., additional, Trump, Benjamin F., additional, Aureli, G., additional, Lauria, A., additional, Rizzotti, M., additional, Bahr, G. F., additional, Wied, G. L., additional, Bartels, P., additional, Bajusz, E., additional, Barer, R., additional, Barka, Tibor, additional, Barnard, Eric A., additional, Komender, Janusz, additional, Wieckowski, Jan, additional, Barrnett, R. J., additional, Barron, K. D., additional, Koeppen, A. H., additional, Bernsohn, J., additional, Bélanger, Leonard F., additional, Beltrami, Carlo Alberto, additional, Björklund, A., additional, Ehinger, B., additional, Falck, B., additional, Boadle, Margaret C., additional, Bloom, Floyd E., additional, Bona, C., additional, Bradshaw, M., additional, Stroman, S., additional, Monus, L., additional, Budd, G. C., additional, Salpeter, M. M., additional, Bukhonova, A. I., additional, Burnasheva, S. A., additional, Jurzina, G. A., additional, Burt, Alvin M., additional, Chang, Jeffre Y. P., additional, Schatzki, Peter F., additional, Saito, Takuma, additional, Chavin, Walter, additional, Chyle, M., additional, Korych, B., additional, Lojda, Z., additional, Patocka, F., additional, Cohn, Z. A., additional, Conning, D. M., additional, Coutinho, Hélio B., additional, Rocha, Jácia T., additional, Jales, Benjamin F., additional, Cunningham, Lew, additional, Heitsch, Richard, additional, Daneholt, B., additional, Edström, J.-E., additional, Danilova, L. V., additional, Rokhlenko, K. D., additional, Dauwalder, M., additional, Whaley, W. G., additional, Kephart, J. E., additional, Deitch, Arline D., additional, Sawicki, Stanley G., additional, Godman, Gabriel C., additional, Francesco, Della Corte, additional, Desmet, V. J., additional, Bullens, A.-M., additional, De Groote, J., additional, Heirwegh, K. P. M., additional, Diculescu, I., additional, Onicescu, Doina, additional, Szegly, G., additional, Doane, Winifred W., additional, Donskikh, N. V., additional, Novikov, V. D., additional, Subbotin, M. Ya., additional, Tsirelnikov, N. I., additional, Doolin, Paul F., additional, Birge, Wesley J., additional, Bernard, Droz, additional, Droz, B., additional, Bergeron, M., additional, Drukker, J., additional, Duarte-Escalante, Ovidio, additional, Dubowitz, Victor, additional, Dupraw, E. J., additional, Eckner, Friedrich A. O., additional, Blackstone, Eugene H., additional, Moulder, Peter V., additional, Ehrlich, M. P., additional, Stanley, Ellis, additional, McDonald, J. Ken, additional, Callahan, P. X., additional, Epifanova, O. I., additional, Lomakina, L. Ya., additional, Terskikh, V. V., additional, Ericsson, Jan L. E., additional, Jakobsson, Sten, additional, Eristawi, K. D., additional, Sharashidze, L. K., additional, Sturua, N. S., additional, Fabris, G., additional, Mariuzzi, G. M., additional, Nenci, I., additional, Fahimi, Dariush H., additional, Karnovsky, Morris J., additional, Fand, Sally B., additional, Farquhar, Marilyn G., additional, Felgenhauer, K., additional, Glenner, G. G., additional, Stammler, A., additional, Filkuka, J., additional, Svejda, J., additional, Áubrechtova, V., additional, Filotto, U., additional, Fischbein, J. W., additional, Rutenburg, A. M., additional, Fisher, Donald B., additional, Alessandra, Forni, additional, Nencioni, Torquato, additional, Ballare, Gianfranco, additional, Ludmila, Fotin, additional, Popescu, Maria, additional, Frankfurt, O. S., additional, Friend, Daniel S., additional, Fuchs, B. B., additional, Arutyunov, V. D., additional, Shnaper, A. L., additional, Gabunia, U. A., additional, Shiukashvili, N. N., additional, Gahan, P. B., additional, Anker, P., additional, Stroun, M., additional, McLean, Jean, additional, Galjaard, H., additional, Bootsma, D., additional, Ganina, K. P., additional, Garcia, Alfredo Mariano, additional, Garrett, J. R., additional, Gepts, W., additional, Gregoire, F., additional, Ooms, H., additional, Giuseppe, Gerzeli, additional, Ezio, Giacobini, additional, Hovmark, Stefan, additional, Gilkerson, Seth W., additional, David, Glick, additional, Godlewski, H. G., additional, Huszczuk, A., additional, Penar, Barbara, additional, Goldfischer, Sidney, additional, Sternlieb, Irmin, additional, Goldstone, A., additional, Szabo, E., additional, Koenig, H., additional, Gornak, K. A., additional, Goslar, H. G., additional, Grigoriadis, P., additional, Jaeger, K. H., additional, Gössner, W., additional, Benoit, H., additional, Gracheva, Nina D., additional, Grillo, T. Adesanya Ige, additional, Gropp, A., additional, Gross, U. M., additional, Gueft, Boris, additional, Guha, S., additional, Fouquet, J. P., additional, Håkanson, R., additional, Owman, Ch., additional, Sporrong, B., additional, Hale, A. J., additional, Marshall, D. J., additional, Switsur, V. R., additional, Hanker, Jacob S., additional, Zenker, Nicolas, additional, Morizono, Yoshihisa, additional, Deb, Chandicharan, additional, Seligman, Arnold M., additional, Hardonk, M. J., additional, Elema, J. D., additional, Koudstaal, Joh, additional, Hoedemaeker, Ph. J., additional, Hayashi, Masando, additional, Heller, A., additional, Hernández, F., additional, De Morentin, J. Martinez, additional, Hans-Jürgen, Herrmann, additional, Hershey, Falls B., additional, Hess, H. H., additional, Pope, A., additional, Bass, N. H., additional, Hewitt, J. M., additional, Guigon, M., additional, Bolubasz, J., additional, Himes, M. H., additional, Burdick, C., additional, Hirai, Kei-Ichi, additional, Takamatsu, Hideo, additional, Shunta, Hirose, additional, Hirsch, Hilde E., additional, Hodges, Donald R., additional, Costoff, Allen, additional, McShan, W. H., additional, Holtzman, Eric, additional, Holubar, K., additional, Tappeiner, J., additional, Wolff, K., additional, Hopsu-Havu, Vainö K., additional, Hosannah, Yvonne, additional, Blackwood, Carlton E., additional, Mandl, Ines, additional, Hoskins, Godfrey C., additional, Hugon, J. S., additional, Borgers, M., additional, Hurwitz, Lawrence S., additional, Rubinstein, Lucien J., additional, Ibrahim, M. Z. M., additional, Imura, Shin-Ichi, additional, Takeda, Masanori, additional, Jacobsen, N. O., additional, Jørgensen, P. Leth, additional, Jarrett, A., additional, Claudia, Joandrea-Casian, additional, Prundeanu, Cornelia, additional, Johnson, Anne B., additional, Johnson, Waine C., additional, Alkek, David S., additional, Jongkind, J. F., additional, Swaab, D. F., additional, Jos, J., additional, Junqueira, L. C., additional, Toledo, A. M. Souza, additional, Kaiser, Hans E., additional, Kakari, Sophia, additional, Kalina, Moshe, additional, Bubis, Jose. J., additional, Kamentsky, L. A., additional, Kasten, Frederick H., additional, Kiefer, Gunter G., additional, Sandritter, W., additional, Killander, D., additional, Rigler, R., additional, Yasuo, Kishino, additional, Kobayashi, H., additional, Urano, A., additional, Yokoyama, K., additional, Koelle, George B., additional, Koenig, Harold, additional, Hughes, Charles, additional, Korhonen, Kalevi L., additional, Kramer, M. F., additional, Poort, C., additional, Kreutzberg, Georg W., additional, Künzel, Erich, additional, Tanyolac, Attila, additional, Labella, Frank S., additional, Langley, O. K., additional, Lanza, Giovanni B., additional, Lappano-Colletta, Eleanor Rita, additional, Leblond, C. P., additional, Merzel, J., additional, Cheng, Hazel, additional, Nadler, N. J., additional, Herscovics, Annette A., additional, Lederer, B., additional, Mittermayer, C., additional, Lee, Sin Hang, additional, Torack, Richard M., additional, Lehrer, Gerard M., additional, Bornstein, Murray B., additional, Katzman, Robert, additional, Leites, F. L., additional, Tendetnik, Ju. J., additional, Ruchadse, E. S., additional, Rjadneva, O. E., additional, Leppi, T. John, additional, Kinnison, Patricia A., additional, Gaffney, Susan P., additional, Lev, Robert, additional, Gerard, Andre, additional, de Graef, Jacques, additional, Glass, George B. Jerzy, additional, Lhotka, J. F., additional, Anderson, J. W., additional, Liber, Amour F., additional, Lillie, R. D., additional, Pizzolato, Philip, additional, Lindner, J., additional, Grasedyck, K., additional, Johannes, G., additional, Freytag, G., additional, Gries, G., additional, Lipchina, L. P., additional, Aksyutina, M. S., additional, Yablonovskaya, L. Ya., additional, Lipetz, Jacques, additional, Liu, J. C., additional, Roizin, L., additional, Lodin, Z., additional, Kage, M., additional, Hartman, J., additional, Srajer, J., additional, Lojda, Zdenek, additional, Fric, Premysl, additional, Long, Margaret E., additional, Sommers, Sheldon C., additional, Ken, McDonald J., additional, Ellis, Stanley, additional, McGarry, E. E., additional, Nayak, R., additional, Birch, E., additional, Beck, J. C., additional, McMillan, Paul J., additional, Adeoye, Christopher ’Seinde, additional, Macovschi, O., additional, Maeda, Ryuei, additional, Ihara, Nobuo, additional, Kanazawa, Kokichi, additional, Maeir, David M., additional, Wagner, Lenore, additional, Viviane, Maggi, additional, Franks, L. M., additional, Livingston, D. C., additional, Coombs, M. M., additional, Wilson, Patricia D., additional, Carbonell, A. W., additional, Malyuk, V. I., additional, Romanini, Manfredi, additional, Gabriella, Maria, additional, Fraschini, Annunzia, additional, Porcelli, Franca, additional, Manocha, Sohan L., additional, Shantha, Totada R., additional, Bourne, Geoffrey H., additional, Marques, Dante, additional, Bastos, A. L., additional, Baptista, A. M., additional, Vigario, J. D., additional, Nunes, J. M., additional, Terrinha, A. M., additional, Silva, J. A. F., additional, Masurovsky, E. B., additional, Benitez, H. H., additional, Kim, S-U., additional, Murray, M. R., additional, Matschinsky, F. M., additional, Rutherford, C. L., additional, Guerra, L., additional, Matturri, L., additional, Curri, S., additional, Mayall, Brian H., additional, Melnick, P. J., additional, Mendelsohn, M. L., additional, Conway, T. J., additional, Perry, B., additional, Prewitt, J. M. S., additional, Mercado, Teresa I., additional, Miksche, Jerome P., additional, Misch, Donald W., additional, Misch, Margaret S., additional, Mitchell, J. P., additional, Kiefer, G., additional, Mizuhira, Vinci, additional, Uchida, Kazuko, additional, Amakawa, Takanori, additional, Shindo, Hideo, additional, Totsu, Junichi, additional, Suesada, Ikuo, additional, Mizutani, Akira, additional, Monis, Benito, additional, Candiotti, Alberto, additional, Mori, G., additional, Ingrami, A., additional, Morikawa, Shigeru, additional, Yamamura, Masao, additional, Harada, Takayuki, additional, Hamashima, Yoshihiro, additional, Mullaney, P. F., additional, Dean, P. N., additional, Van Dilla, M. A., additional, Müller, Gerhard, additional, Müller, Otfried, additional, Nakane, Paul K., additional, Neurath, Peter W., additional, Curtis, Zay B., additional, Selles, William, additional, Vetter, Henri G., additional, Norgren, P. E., additional, Novikoff, Alex B., additional, Regina, O‘brien, additional, Ohringer, Philip, additional, Spitaleri, Vincent, additional, Olszewska, M. J., additional, Gabara, B., additional, Konopska, L., additional, Parfanovich, M. I., additional, Sokolov, N. N., additional, Berezina, O. N., additional, Fadeeva, L. L., additional, Pauly, John E., additional, Scheving, Lawrence E., additional, Pearse, A. G. E., additional, Pearson, Bjarne, additional, Bennett, William, additional, Esterly, John R., additional, Standen, Alfred C., additional, Pelc, S. R., additional, Viola-Magni, M. P., additional, Antti, Penttilä, additional, Perez, Vernon J., additional, Moore, Blake W., additional, Peters, Theodore, additional, Danzi, J. Thomas, additional, Ashley, Charles A., additional, Petrova, A. S., additional, Probatova, N. A., additional, Philippens, Karel, additional, Pilgrim, C., additional, Pollock, B. M., additional, Presnov, M. A., additional, Preston, Kendall, additional, Preto, V. Parvis, additional, Cisotti, F., additional, Mazza, G. E., additional, Prewitt, Judith M. S., additional, Mendelsohn, Mortimer L., additional, Pryse-Davis, John, additional, Sandler, Merton, additional, Quay, W. B., additional, Raikhlin, N. T., additional, Rasch, Ellen M., additional, Riecken, E. O., additional, Goebell, H., additional, Bode, C., additional, Rigatuso, Joseph L., additional, Ringertz, N. R., additional, Bolund, L., additional, Carl, Ritter, additional, Thorell, Bo, additional, Balduini, C., additional, Castellani, A. A., additional, Rosenbaum, Robert M., additional, Rosene, Gordon L., additional, Rossi, Ferdinando, additional, Rost, F. W. D., additional, Roth, Daniel, additional, Ruch, Fritz, additional, Ruddle, Frank H., additional, Lubs, Herbert A., additional, Ledley, Robert S., additional, Shows, Thomas B., additional, Roderick, Thomas H., additional, Kim, H., additional, Brodie, E., additional, Fischbein, J., additional, Rosales, C. L., additional, Sadauskas, P., additional, Luksys, L., additional, Dabkevcius, V., additional, Sakharova, A. V., additional, Sakharov, D. A., additional, Samosudova, N. V., additional, Ogieveckaja, M. M., additional, Kalamkarova, M. B., additional, Sandler, Maurice, additional, Santti, R. S., additional, Hopsu-Havu, V. K., additional, Sasaki, Mitsuo, additional, Takeuchi, Tadao, additional, Satir, P., additional, Schauer, Alfred, additional, Scher, Stanley, additional, Haley, Patricia L., additional, Schiebler, T. H., additional, Schiemer, Hans-Georg, additional, Schlüns, Jürgen, additional, Schuster, F. L., additional, Hershenov, B., additional, Scott, J. E., additional, Scott, T. Gilbert, additional, Seno, Satimaru, additional, Yokomura, Ei-ichi, additional, Itoh, Nobutaka, additional, Yamamoto, Michio, additional, Shungskaya, V. E., additional, Enenko, S. O., additional, Lukyanova, L. D., additional, Sarch, E. N., additional, Eli, Shuter, additional, Jungalwala, Firoze, additional, Robins, Eli, additional, Slerakowska, Halina, additional, Silverman, L., additional, Glick, D., additional, Simard, A., additional, Daoust, R., additional, Smith, Edgar E., additional, Smith, R. E., additional, Fishman, William H., additional, Henzl, Milan, additional, Sobel, Harold J., additional, Avrin, Erna, additional, Sorokin, Helen P., additional, Sorokin, Sergei, additional, Sorokin, Sergei P., additional, Squier, C. A., additional, Waterhouse, J. P., additional, Steinbach, Günter, additional, Steplewski, Zenon, additional, Stitnimankarn, Tinrat, additional, Stoward, Peter J., additional, Straus, W., additional, Stumpf, Walter E., additional, Roth, Lloyd J., additional, Sylvèn, B., additional, Kanamura, Shinsuke, additional, Templeton, McCormick, additional, Tewari, H. B., additional, Tyagi, H. R., additional, Thalmann, R., additional, Glismann, L., additional, Thomas, E., additional, Tice, Lois W., additional, Tixier-Vidal, A., additional, Törö, I., additional, Bacsy, E., additional, Vadasz, Gy., additional, Rappay, Gy., additional, Tsou, K. C., additional, Chang, Mildred Y., additional, Matsukawa, S., additional, Goodwin, Cleon, additional, Lynm, Dwo, additional, Seamond, Bette, additional, Van der Ploeg, M., additional, Van Duijn, P., additional, Coulter, J. R., additional, Pascoe, E., additional, Van Fleet, D. S., additional, Van Houten, Wiecher H., additional, Vecher, A. S., additional, Masko, A. A., additional, Predkel, K. I., additional, Reshetnikov, V. N., additional, Tchaika, M. T., additional, Velican, C., additional, Velican, Doina, additional, Vendrely, C., additional, Lageron, A., additional, Tournier, P., additional, Vialli, Maffo, additional, Prenna, Giovanni, additional, Vilter, Voldemar, additional, Vittek, Josef, additional, Vogt, Arnold, additional, Vollrath, L., additional, Von Mayersbach, Heinz, additional, Andrzej, Vorbrodt, additional, Wächtler, Klaus, additional, Wakabayashi, Katsumi, additional, Bun-Ichis, Tamaoki, additional, Niel, Wald, additional, Ranshaw, Russell, additional, Weller, Roy O., additional, Welsch, Ulrich, additional, Werner, Gottfried, additional, Vick, Williams, additional, Morriss, Fran, additional, Willighagen, R. G. J., additional, Wilson, Barry W., additional, Wohlrab, Frank, additional, Wolf, Paul L., additional, Horwitz, Jerome P., additional, Freisler, Josef V., additional, Von der Muehll, Elisabeth, additional, Vazquez, Janice, additional, Wolman, Moshe, additional, Wolman, M., additional, Kalina, M., additional, Bubis, J. J., additional, Yamada, Masaoki, additional, Iwata, Sunao, additional, Yamaguchi, Hisao, additional, Yataganas, X., additional, Gahrton, G., additional, Thorell, B., additional, Young, Ian T., additional, Zaccheo, D., additional, Grossi, C. E., additional, Genta, V., additional, Riva, A., additional, Zacks, S. I., additional, Sheff, M. F., additional, Zamfirescu-Gheorghiu, M., additional, Serban, M., additional, Vladescu, C., additional, Chirulescu, Z., additional, Marcus, N., additional, Zelenin, A. V., additional, Kirianova, E. A., additional, Stepanova, N. G., additional, Zeuthen, Erik, additional, Zimmermann, Horst, additional, Zugibe, Frederick T., additional, Abrahamson, Dean E., additional, Anderson, Norman G., additional, Caspersson, Törbjorn, additional, Richard, Cornell, additional, Dougherty, William, additional, Jirasek, J. E., additional, Jonsson, Gösta, additional, Leske, Regina, additional, Moyer, Frank H., additional, Schneider, Walter C., additional, Siegel, Howard I., additional, Sternberger, Ludwig, additional, Osserman, Elliott F., additional, Weinstock, A., additional, Kåroly, Balogh, additional, and Enesco, Hildegard E., additional
- Published
- 1968
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7. Inhibition of DNA synthesis in erythroleukemic cells by a liver protein fraction
- Author
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Viola-Magni, M. P. and Ricerchi, P.
- Published
- 1983
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8. Effetto del ciprofibrato sulla sfingomielinasi neutra cromatinica
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Albi, E., Pieroni, S., Sartori, Claudia, and VIOLA MAGNI, M. P.
- Published
- 2000
9. Hemoglobin stain in fixed eryhroleukemic cells: an improvement of the method combined with autoradiography
- Author
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Serenelli, Giovanna and VIOLA MAGNI, M. P.
- Subjects
Erythroleukemic cells ,Hemoglobin ,Differentiation - Published
- 1990
10. Cytokines and joint injury W. B. van den Berg and P. Moissec (eds), Birkhauser Verlag AG Basel, 250 pp., CHF 228.00/140.00, ISBN 3-7643-7001-7 (2004)
- Author
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Viola-Magni, M-P., primary
- Published
- 2005
- Full Text
- View/download PDF
11. Mitoses in the adrenal medullary cells
- Author
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Malvaldi, G., Mencacci, P., and Viola-Magni, M. P.
- Published
- 1968
- Full Text
- View/download PDF
12. Postnatal variations of the dry mass of neuronal nuclei of the spinal cord in rat and guinea-pig
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Viola-Magni, M. P. and Puccinelli, E.
- Published
- 1966
- Full Text
- View/download PDF
13. Changes in oncogene expression in ascite tumour cells during ageing
- Author
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Biagetti, M., primary, Fazia, M. A. Della, additional, Servillo, G., additional, and Viola-Magni, M. P., additional
- Published
- 1994
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- View/download PDF
14. Age-related changes in the cell proliferation of ATPC+mouse ascites tumour
- Author
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Viola-Magni, M. P., primary and Biagetti, M., additional
- Published
- 1991
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15. Phospholipids in chromatin: Incorporation of [32P]O.
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Viola-Magni, M. P., Gahan, P. B., Albi, E., Iapoce, R., and Gentilucci, P. F.
- Published
- 1986
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16. Phospholipids in plant and animal chromatin.
- Author
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Viola-Magni, M. P., Gahan, P. B., and Pacy, J.
- Published
- 1985
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17. Regulatory mechanisms of hepatic phosphorylase in fetal and neonatal livers of rats.
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BIONDI, R. and VIOLA-MAGNI, M. P.
- Published
- 1977
- Full Text
- View/download PDF
18. Isolation and Analysis of Low Molecular Weight DNA Fraction by Electrophoretic and Chromatographic Techniques.
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Rossi, R. and Viola-Magni, M. P.
- Published
- 1978
- Full Text
- View/download PDF
19. Antiphospholipid Antibodies in Patients with Cancer
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Pugliese, L., Bernardini, I., Pacifico, E., Viola-Magni, M., and Albi, E.
- Abstract
Antiphospholipid antibodies are generally associated with Antiphospholipid Syndrome, which can occur as a primary disorder or may be secondary to connective tissue disease or tumour. The presence of antiphospholipid antibodies in patients with tumour disease is responsible for thrombotic complications. In a population of 53 tumor patients with positive carcinoembryonic antigen CEA, carbohydrate antigen CA19.9, CA125 and CA15.3 markers, IgM and IgG anticardiolipin and antiphosphatidylinositol were detected by solid-phase immunoassays. Our results show that moderate or high levels of antiphospholipid antibodies are present in a great number of patients with CEA and CA19.9 markers, suggesting a specific association with gastroenteric tumors. By testing for antiphosphatidylinositol antibodies, many patients not evidenced by the standard anticardiolipin assay were found to be antiphospholipid-positive. The analysis of antiphosphatidylinositol antibodies as a diagnostic tool in gastroenteric cancer to highlight patients with the risk of thromboembolic complications is discussed.
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- 2006
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- View/download PDF
20. Synthesis and turnover of DNA in hepatocytes of neonatal rats.
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Viola-Magni, M. P.
- Published
- 1972
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21. PHOSPHOLIPIDS AND NUCLEAR RNA
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ALBI, E., MICHELI, M., and VIOLA MAGNI, M. P.
- Abstract
It has been demonstrated that in hepatocyte nuclei the chromatin phospholipid fraction is localized near the RNA in decondensed chromatin. The aim of the present study was to see if there is any linkage between phospholipids and other nuclear components. Isolated hepatocyte nuclei and nuclear membranes were treated with deoxyribonuclease and ribonuclease. No loss of phospholipids was observed after DNA digestion, whereas 48% was lost following enzymatic RNA removal. This loss of phospholipids, localized either near the membrane or inside the nucleus, was not homogeneous for all phospholipids: phosphatidylserine and sphingomyelin being the most affected. It can be concluded that 48% of nuclear phospholipids, in particular sphingomyelin, is lost with RNA removal. This result is discussed in view of a possible role of phospholipids in DNA synthesis and RNA transcription.
- Published
- 1996
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22. Synthesis of chromatin phospholipids
- Author
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Viola-Magni, M. P., Gahan, P. B., Elisabetta Albi, Iapoce, R., and Gentilucci, P. F.
- Subjects
Cell Nucleus ,DNA ,Animals ,Autoradiography ,Chromatin ,Interphase ,Kinetics ,Liver ,Liver Regeneration ,Microsomes, Phospholipids ,RNA ,Rats ,Phosphates ,Microsomes ,Microsomes, Liver ,Phospholipids - Abstract
The presence of a phospholipid fraction associated with chromatin has been demonstrated by biochemical technique in rat hepatocytes. The composition of this fraction determined by chromatography with respect to that of the nuclei is characterized by low content of phosphatidylserine and high content in phosphatidylethanolamine. Also the synthesis and turnover studied after injection of [32P]O4(2-) show a different behaviour: the peak of activity is after 6 hrs in nuclei and microsomes, whereas in chromatin it occurs after 9 hrs. A second peak is evident after 24 hrs in chromatin and microsome phospholipids. Differences have been also shown by analyzing the single phospholipid radioactivity in time. The behaviour of chromatin phospholipids has also been studied during DNA premitotic synthesis in regenerating liver. It has been shown that there is no difference in synthesis in relation to that of DNA in nuclear phospholipids, whereas the specific activity of chromatin phospholipids begins to increase twelve hours after hepatectomy and continues throughout the period of the first mitotic wave, thus bringing to a summation with the beginning of the second wave. The role of this phospholipid fraction in relation to DNA synthesis and gene expression is discussed.
23. Regulatory mechanisms of hepatic phosphorylase in fetal and neonatal livers of rats.
- Author
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Biondi, R, primary and Viola-Magni, M P, additional
- Published
- 1977
- Full Text
- View/download PDF
24. Phospholipids in chromatin: Incorporation of [32P]O42− in different subcellular fractions of hepatocytes
- Author
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Viola-Magni, M. P., primary, Gahan, P. B., additional, Albi, E., additional, Iapoce, R., additional, and Gentilucci, P. F., additional
- Published
- 1986
- Full Text
- View/download PDF
25. Autoradiographic Study of the Turnover of Chromatin-Associated Phospholipids inVicia FabaL.
- Author
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Gahan, P. B., primary, Viola-Magni, M. P., additional, and Cave, C. F., additional
- Published
- 1986
- Full Text
- View/download PDF
26. Isolation and Analysis of Low Molecular Weight DNA Fraction by Electrophoretic and Chromatographic Techniques
- Author
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Rossi, R., primary and Viola-Magni, M. P., additional
- Published
- 1978
- Full Text
- View/download PDF
27. A scanning integrating histophotometer
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Benedetti, P A, primary and Viola-Magni, M P, additional
- Published
- 1966
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- View/download PDF
28. Progress towards the 'Golden Age' of biotechnology.
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Gartland KM, Bruschi F, Dundar M, Gahan PB, Viola Magni Mp, and Akbarova Y
- Subjects
- Agriculture trends, Animals, Antimalarials, Biofuels, Biotechnology economics, Carbohydrates, Climate Change, Droughts, Environment, Epigenomics, Food Supply methods, Humans, Plastics, Proteins metabolism, Biotechnology trends
- Abstract
Biotechnology uses substances, materials or extracts derived from living cells, employing 22 million Europeans in a € 1.5 Tn endeavour, being the premier global economic growth opportunity this century. Significant advances have been made in red biotechnology using pharmaceutically and medically relevant applications, green biotechnology developing agricultural and environmental tools and white biotechnology serving industrial scale uses, frequently as process feedstocks. Red biotechnology has delivered dramatic improvements in controlling human disease, from antibiotics to overcome bacterial infections to anti-HIV/AIDS pharmaceuticals such as azidothymidine (AZT), anti-malarial compounds and novel vaccines saving millions of lives. Green biotechnology has dramatically increased food production through Agrobacterium and biolistic genetic modifications for the development of 'Golden Rice', pathogen resistant crops expressing crystal toxin genes, drought resistance and cold tolerance to extend growth range. The burgeoning area of white biotechnology has delivered bio-plastics, low temperature enzyme detergents and a host of feedstock materials for industrial processes such as modified starches, without which our everyday lives would be much more complex. Biotechnological applications can bridge these categories, by modifying energy crops properties, or analysing circulating nucleic acid elements, bringing benefits for all, through increased food production, supporting climate change adaptation and the low carbon economy, or novel diagnostics impacting on personalized medicine and genetic disease. Cross-cutting technologies such as PCR, novel sequencing tools, bioinformatics, transcriptomics and epigenetics are in the vanguard of biotechnological progress leading to an ever-increasing breadth of applications. Biotechnology will deliver solutions to unimagined problems, providing food security, health and well-being to mankind for centuries to come., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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- View/download PDF
29. Biotechnology worldwide and the 'European Biotechnology Thematic Network' Association (EBTNA).
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Bruschi F, Dundar M, Gahan PB, Gartland K, Szente M, Viola-Magni MP, and Akbarova Y
- Subjects
- Biotechnology economics, Congresses as Topic, Europe, Humans, Industry, Public-Private Sector Partnerships, Research, Universities, Biotechnology education, Biotechnology ethics, Biotechnology trends
- Abstract
The European Biotechnology Congress 2011 held under the auspices of the European Biotechnology Thematic Network Association (EBTNA) in conjunction with the Turkish Medical Genetics Association brings together a broad spectrum of biotechnologists from around the world. The subsequent abstracts indicate the manner in which biotechnology has permeated all aspects of research from the basic sciences through to small and medium enterprises and major industries. The brief statements before the presentation of the abstracts aim to introduce not only Biotechnology in general and its importance around the world, but also the European Biotechnology Thematic Network Association and its aims especially within the framework of education and ethics in biotechnology., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
30. Low levels of serum cholesterol/phospholipids are associated with the antiphospholipid antibodies in monoclonal gammopathy.
- Author
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Pugliese L, Bernardini I, Viola-Magni MP, and Albi E
- Subjects
- Aged, Cardiolipins immunology, Cholesterol Esters blood, Female, Humans, Immunoglobulin A analysis, Immunoglobulin A immunology, Male, Middle Aged, Phosphatidylinositols immunology, Phospholipids immunology, Antibodies, Antiphospholipid immunology, Cholesterol blood, Paraproteinemias blood, Paraproteinemias immunology, Phospholipids blood
- Abstract
A decrease in cholesterol blood level, not due to a decrease synthesis by the liver, has been observed in patients suffering from tumors. In this work cholesterol blood was evaluated in patients affected by monoclonal gammopathy who were not subjected to any treatment. The blood of 25 patients were analyzed for protein and lipid content. Patients were divided according to the gamma protein content into three groups, and it was demonstrated that the group with high levels of gamma proteins presented a strong decrease in blood cholesterol and phospholipids. In these patients the presence of antibodies against phospholipids by using cardiolipin and phosphatidylinositol as antigens has also been demonstrated. The antibodies were rare in patients with a low content of gamma proteins and normal level of lipids, but the frequency was more than 80% in patients with low blood lipid levels.
- Published
- 2006
- Full Text
- View/download PDF
31. The role of intranuclear lipids.
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Albi E and Viola Magni MP
- Subjects
- Animals, Apoptosis physiology, Cell Differentiation physiology, Cell Nucleus metabolism, Cell Proliferation, Cholesterol metabolism, Cholesterol physiology, Chromatin chemistry, Chromatin metabolism, Humans, Lipid Metabolism, Lipids analysis, Phospholipids metabolism, Phospholipids physiology, Plasmalogens metabolism, Plasmalogens physiology, RNA metabolism, Sphingomyelins metabolism, Sphingomyelins physiology, Intranuclear Space metabolism, Lipids physiology
- Abstract
The presence of phospholipids as a component of chromatin is now well documented and many enzymes such as sphingomyelinase, sphingomyelin-synthase, reverse sphingomyelin-synthase and phosphatidylcholine-dependent phospholipase C have been described and characterised. Other lipids were demonstrated inside the nucleus especially plasmalogens and cholesterol. The chromatin phospholipids, comprising 10% of that present in the nucleus, show a different metabolism with respect to those present in either microsomes or in nuclear membranes; they increase also during the DNA duplication as shown during both liver regeneration and cell maturation. They appear localised near newly synthesized RNA in decondensed chromatin. Digestion of chromatin with RNase, but not with DNase, causes a loss of phospholipids. The composition of the chromatin phospholipid fraction shows an enrichment in sphingomyelin and phosphatidylserine. In this review the behaviour of single lipids in relation to cell proliferation, cell differentiation and apoptosis is described. Sphingomyelin, the lipid most represented in chromatin with respect to microsomes and nuclear membranes, is localised near to newly synthesized RNA, its presence appearing to protect RNA from RNase digestion. This effect is reversed by sphingomyelinase which digests sphingomyelin and, as a consequence, RNA may be hydrolysed. The amount of sphingomyelin is restored by sphingomyelin-synthase. Sphingomyelin increases during the differentiation process and apoptosis. An increase of sphingomyelinase with consequent decrease in sphingomyelin is observed at the beginning of S-phase of the cell cycle. A possible role in stabilising the DNA double helix is indicated. Phosphatidylserine behaves similarly during differentiation and appears to stimulate both RNA and DNA polymerases. Phosphatidylcholine is implicated in cell proliferation through the activation of intranuclear phosphatidylcholine-dependent phospholipase C and diacylglycerol production. The increase in diacylglycerol stimulates phosphatidylcholine synthesis through the major pathway from cytidyltriphosphate. An inhibition of phosphatidylcholine synthesis is responsible for the initiation of apoptosis. The presence of reverse sphingomyelin-synthase favours the formation of phosphatidylcholine, the donor of phosphorylcholine, from sphingomyelin. Little information has been reported for phospatidylethanolamine, but phosphtidylinositol appears to influence cell differentiation and proliferation. This last effect is due to the action of two enzymes: PI-PLCss1 having a role in the onset of DNA synthesis and PC-PLCgamma1 acting in G2 transit. Phosphoinositides also may have an important role: in membrane-stripped nuclei isolated from mitogen stimulated cells a decrease in PIP and PIP2 followed by an increase in diacylglycerol and a translocation of protein kinase C inside the nucleus is observed. On the other hand, overexpression of the enzyme inositol polysphosphate-1-phosphatase reduced DNA synthesis by 50%. Nevertheless, an enhanced rate of phosphorylation has been demonstrated in cells induced to differentiate. These molecules probably favour RNA transcription, counteracting the inhibition of H1 on RNA polymerase II. Plasmalogens were demonstrated in the nucleus and their increase favours the increased activity of phosphatidylcholine-dependent phospholipase C when DNA synthesis starts. Moreover, two forms of cholesterol has been described in chromatin: one, a less soluble sphingomyelin-linked form and a free fraction. Cholesterol increases during liver regeneration, first as a linked fraction and then, when DNA synthesis starts, as a free fraction. The changes of these components have been summarised in relation to cell function in order to give an overview of their possible roles in the different phases of cell duplication and their influence on cell differentiation and during apoptosis. Finally, the relevance of these molecules as intranuclear signals is discussed and future directions are indicated in clarifying pathological process such as tumour cell transformation and the possibility in finding new therapeutic tools.
- Published
- 2004
- Full Text
- View/download PDF
32. Chromatin-associated sphingomyelin: metabolism in relation to cell function.
- Author
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Albi E and Viola-Magni MP
- Subjects
- Animals, Apoptosis physiology, Cell Differentiation physiology, Cell Division physiology, Phospholipids analysis, RNA metabolism, Rats, Ribonucleases metabolism, Sphingomyelin Phosphodiesterase analysis, Sphingomyelin Phosphodiesterase metabolism, Sphingomyelins analysis, Transferases (Other Substituted Phosphate Groups) analysis, Transferases (Other Substituted Phosphate Groups) metabolism, Chromatin metabolism, Sphingomyelins metabolism
- Abstract
After the first histochemical demonstration by Chayen and Gahan of the presence of phospholipids and especially of sphingomyelin in chromatin, this became the object of long debate and of contradictory results. The general conclusion was that the presence of phospholipids may due to contamination during the isolation of chromatin. More recently the existence of a phospholipid chromatin fraction was confirmed by demonstrating that isolated hepatocyte nuclei, labelled by saturated and unsaturated radioiodination method, showed the presence of radioactivity only in the membrane and not in the isolated chromatin. The phospholipid composition showed an enrichment in sphingomyelin which increased during hepatocyte maturation or erythroleukemic cell differentiation induced by DMSO. A decrease in sphingomyelin was observed at the beginning of the S-phase in regenerating liver or in cultured proliferating cells. These changes were due to the presence of sphingomyelinase and sphingomyelin synthase in the chromatin, the activity of which paralleled the variation in sphingomyelin content. The sphingomyelin was co-localized with RNA as shown by biochemical and electron microscopy methods. Using bromo-uridine it was demonstrated that labelled RNA and sphingomyelin were present in actively transcribing nuclear regions. Isolated nuclear complexes after DNase and RNase digestion contained not only protein, but also RNA and sphingomyelin. After hydrolysis of sphingomyelin the RNAse-resistant RNA becomes RNAse sensitive. It can therefore be concluded that sphingomyelin and the related enzymes are present in the chromatin; sphingomyelin may have a role in RNA transcription protecting RNA by RNAse digestion before its transfer to the cytoplasm., (Copyright 2003 John Wiley & Sons, Ltd.)
- Published
- 2003
- Full Text
- View/download PDF
33. Chromatin sphingomyelin changes in cell proliferation and/or apoptosis induced by ciprofibrate.
- Author
-
Albi E, Pieroni S, Viola Magni MP, and Sartori C
- Subjects
- Animals, Cell Division physiology, Enzyme Activation, Fibric Acids, Liver anatomy & histology, Liver drug effects, Liver growth & development, Liver physiology, Male, Organ Size drug effects, Phosphatidylcholines metabolism, Rats, Rats, Wistar, Sphingomyelin Phosphodiesterase metabolism, Apoptosis physiology, Chromatin metabolism, Clofibric Acid analogs & derivatives, Clofibric Acid pharmacology, Sphingomyelins physiology
- Abstract
It has been shown that neutral-sphingomyelinase and sphingomyelin-synthase activities are present in chromatin and they modify the sphingomyelin (SM) content. The activity of the first enzyme is stimulated and the second inhibited, when the hepatocytes enter into the S-phase after partial hepatectomy, thus suggesting that ceramide may have a pivotal role in cell proliferation. An opposite function was attributed to ceramide in hepatocytes which undergo apoptosis after lobular ligature. In order to clarify this point, a model was developed in which the same liver cells undergo proliferation followed by induced apoptosis. To this purpose, the rats were treated for 7 days with ciprofibrate and then left without treatment for 4 days. During the treatment, the peroxisome enzyme markers increase their activity and the number of proliferating cells increases, reaching a maximum after 3 days of treatment, as shown by the number of cells positive for the proliferating cell nuclear antigen. At the same time, the chromatin sphingomyelinase activity reaches the maximum, while a similar increase is not found in the cytoplasm or in the isolated nuclei. On the contrary, SM-synthase activity is depressed in chromatin, but not in the nuclei in which a peak is shown after 3 days of ciprofibrate treatment. After drug withdrawal, the hepatocytes undergo apoptosis as confirmed by the increase of Bax and tissue transglutaminase (tTGase) expression; the chromatin SM increases as a consequence of an increase of SM-synthase activity. It can be hypothesised that chromatin SM may have a role in cell duplication by influencing the chromatin structure stability., (Copyright 2003 Wiley-Liss, Inc.)
- Published
- 2003
- Full Text
- View/download PDF
34. Antiphosphatidylinositol antibody in deep venous thrombosis patients.
- Author
-
Panarelli P, Viola-Magni MP, and Albi E
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Chi-Square Distribution, Female, Humans, Immunoglobulin G blood, Male, Middle Aged, Phosphatidylinositols blood, Venous Thrombosis immunology, Antibodies, Antiphospholipid blood, Phosphatidylinositols immunology, Venous Thrombosis blood
- Abstract
Antiphospholipid antibodies are a heterogeneous group of immunoglobulins with specificity for a number of phospholipids, phospholipid-binding proteins and phospholipid-protein complexes. The association between antiphospholipid antibodies and a variety of pathologic disorders, such as arterial and venous thrombosis and recurrent pregnancy loss is recognized as Antiphospholipid Syndrome. The immunoassay currently used to detect antiphospholipid antibodies is the anticardiolipin test. Anticardiolipin antibodies are believed to be polyspecific antibodies that cross-react with all the anionic phospholipids. Therefore, testing only for anticardiolipin antibodies does not always permit detection of all antiphospholipid antibodies, specially when only IgG are evaluated. In a selected population of 74 idiopathic and secondary deep venous thrombosis patients, IgG anticardiolipin, antiphosphatidylinositol and antiphosphatidylserine antibodies were detected by solid-phase immunoassays. Our results show that by testing for each antiphospholipid family, many patients, not evidenced by the standard anticardiolipin assay, were found to be antiphospholipid-positive. The anticardiolipin positive patients have always low, moderate or high levels of antiphospholipid antibodies, suggesting that the antiphospholipid positivity is predictive of anticardiolipin positivity. It should be noted that the patients with only antiphosphatidylinositol positive antibody have a story of nervous system pathology. The meaning of these results is at present under discussion.
- Published
- 2003
- Full Text
- View/download PDF
35. In utero partial liver resection in the rabbit model: a study on fetal tissue regeneration.
- Author
-
Patricolo M, Zangari A, Paolocci N, Magni F, Viola-Magni MP, Hernandez-Mena LA, Capuano L, and Rivosecchi M
- Subjects
- Animals, Cesarean Section, Female, Gestational Age, Leukocyte Count, Liver cytology, Lymphocytes, Neutrophils, Organ Size, Phagocytes, Pregnancy, Rabbits, Fetus surgery, Hepatectomy, Liver embryology, Liver Regeneration
- Abstract
In this study we developed a model of in vivo intrauterine partial liver resection in the fetal rabbit to analyze fetal liver regeneration. After intravenous anesthesia, 12 time-dated pregnant, California rabbits underwent a midline laparotomy and minimal hysterotomy at 24-25 days of gestational age. One fetus was exposed from each pregnant doe and the fetal liver was partially resected. Cesarean sections were performed 24, 48 and 72 h and 4 days after surgery. Three fetuses operated at 24 days of gestational age and 3 fetuses operated at 25 days were alive at retrieval. The fetuses and the sampled livers were weighed at retrieval and fetal liver weight showed a well-maintained value in all cases. Fetal livers were processed for the common histologic stains. Lymphocytes, polymorphonuclear leukocytes and phagocytes were counted from sections obtained in areas close to the edge of resection. Inflammatory cells showed a peculiar pattern of infiltration at different stages of repair, with a constantly increased number of phagocytes peaking 48 h after resection. Fetal liver seems to present a specific pattern of repair that differs from both the adult liver and other fetal tissues healing after injury.
- Published
- 1997
- Full Text
- View/download PDF
36. Choline base exchange activity in rat hepatocyte nuclei and nuclear membranes.
- Author
-
Albi E and Viola-Magni MP
- Subjects
- Animals, Calcium metabolism, Female, Liver ultrastructure, Male, Microsomes, Liver metabolism, Rats, Rats, Sprague-Dawley, Cell Nucleus metabolism, Choline metabolism, Liver metabolism, Nuclear Envelope metabolism
- Abstract
Previous investigations have demonstrated the presence of phospholipids as a component of chromatin; however the mechanism of their synthesis, namely if they are synthesized in the nuclei or in the cytoplasm (microsomal fraction), from where they may eventually be transported to the nucleus, has not yet been clarified. The phosphatidylcholine, for example, can be formed, albeit in a limited amount, by an interconversion reaction between bases. The aim of the present research was to ascertain the presence of the enzyme complex responsible for this reaction in hepatocyte nuclei and in isolated nuclear membrane. The incorporation of [14C]-choline in phosphatidylcholine was assayed in microsomes, hepatocyte nuclei, liver nuclei and nuclear membranes of rat liver. The reaction was Ca(2+)-dependent and the specific activity was higher in microsomes but was present, albeit at a low level, also in nuclei and in nuclear membranes. Possible contaminations were excluded by specific microsomal markers and by the reaction time course. In fact, the nuclear reaction reached the maximum level slowly with respect to microsomes. Since the phosphatidylcholine extracted from the nuclei show an enrichment in unsaturated fatty acids of monoenoic fraction, such as oleic acid, the difference in reaction kinetics has been tentatively explained as due to the phosphatidylcholine fatty acid content. The presence of this base exchange enzyme complex may allow a fast change in chromatin phospholipid composition.
- Published
- 1997
- Full Text
- View/download PDF
37. CD44 protein in glioma rat cell culture.
- Author
-
Serenelli G, Frascarelli M, Petturiti G, Viola-Magni MP, and Tognellini R
- Subjects
- Animals, Flow Cytometry, Immunohistochemistry, Plants, Medicinal physiology, Rats, Tea physiology, Tumor Cells, Cultured, Glioma metabolism, Hyaluronan Receptors metabolism
- Published
- 1997
38. Apoptosis induced in glioma rat cells cultivated in the presence of a medicinal infusion of green tea.
- Author
-
Serenelli G, Petturiti G, Tognellini R, Frascarelli M, Sabbagh M, and Viola-Magni MP
- Subjects
- Animals, Cell Cycle drug effects, Dose-Response Relationship, Drug, Flow Cytometry, Rats, Time Factors, Tumor Cells, Cultured, Apoptosis, Glioma pathology, Plants, Medicinal physiology, Tea
- Published
- 1997
39. [Hepatic resection in the fetal rabbit. Histologic comparison of tissue regeneration in the fetus versus the adult].
- Author
-
Patricolo M, Paolocci N, Zangari A, Antonica A, Rossi L, Magni F, Viola-Magni MP, Caione P, Lais A, and Rivosecchi M
- Subjects
- Animals, Female, Liver pathology, Liver surgery, Pregnancy, Rabbits, Reproducibility of Results, Fetus physiology, Hepatectomy, Liver embryology, Liver physiology, Liver Regeneration, Pregnancy, Animal physiology
- Abstract
Fetal tissues present peculiar features of repair after injury. Although the development of fetal hepatocytes have already been studied in vitro and in transplant models, an in vivo study of fetal liver regeneration is still missed in the literature, to the best of our knowledge. Eight time-dated pregnant California rabbits (23, 24, 25, 30 days of gestational age) and 2 adult male California rabbits were anesthetized following a standardized i.v. protocol (ketamine 50 mg/kg; xilazine 5 mg/kg; propiopromazine 0.75 mg/kg; spontaneous breathing; no anesthetic gas). All the pregnant does underwent a midline laparotomy and a minimal hysterotomy to approach a fetus per each animal. In 2 cases, 1 fetus was delivered and prior to sacrifice the fetal liver was sampled in toto (30 days of gestational age). These pregnancies were allowed to continue to term and were uneventful with a full-term spontaneous delivery of the remaining fetuses. In the other 6 pregnancies, after the hysterotomy, the fetal abdomen was entered through a right-sided longitudinal incision and the liver was partially resected by thermocauterization. Fetal abdomen was closed in 1 layer (non absorbable suture 7-0). The fetus was then returned in the uterus and, after amniotic fluid restoration with warmed saline, the hysterotomy was sutured in double layer (polyglycolic 5-0). Maternal abdomen was closed in 1 layer (polyglycolic 4-0) and the skin in a continuous overlying fashion (silk 3-0). The abdominal cavity of the 2 adult male rabbits was entered through a right subcostal incision. Partial liver resection was performed, and abdominal and skin closure followed the same techniques used for the pregnant does. The treated livers were then sampled in toto at 24, 48, 72 hrs and 4 days after surgery from the fetuses, and at 7 days from the adult rabbits. Histological stains were: H & E; Van Gieson; Masson; Alcian Bleu; PAS. Fetal histology showed a low inflammatory reaction poor in PMN cells with minimal deposition of collagen and a high amount of glycogen in the hepatocytes. The inflammatory response to resection was much more evident in the adult samples as much as the abundant intra and extra-cellular deposition of collagen associated to a minor amount of intracellular glycogen. The peculiar features of liver regeneration in the fetus, deserve further experimental studies.
- Published
- 1996
40. GM2 activator protein expression in mouse tissues.
- Author
-
Beccari T, Palmerini CA, Servillo G, Della Fazia MA, Viola Magni MP, and Orlacchio A
- Subjects
- Animals, Brain metabolism, G(M2) Activator Protein, Kidney metabolism, Male, Mice, Mice, Inbred BALB C, Proteins metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Spleen metabolism, Testis metabolism, Tissue Distribution, G(M2) Ganglioside metabolism, Proteins genetics
- Abstract
The GM2 activator protein is an essential cofactor of hexosaminidase A in the degradation of GM2 ganglioside and it is responsible for the variant AB of GM2 gangliosidosis in man. In this study GM2 activator protein and its mRNA were determined in different mouse tissues. It was found that this protein is expressed mostly in the spleen and testis followed by brain and kidney which represent the main source in man. It is also interesting that in mouse testis there is a higher expression of the alpha subunit of hexosaminidase, thus suggesting a relationship between alpha subunit and GM2 activator protein. Furthermore the results indicate that the expression of GM2 activator protein is regulated, at least in part, at the transcriptional level.
- Published
- 1994
- Full Text
- View/download PDF
41. Different expression of beta-N-acetylhexosaminidase in mouse tissues.
- Author
-
Della Fazia MA, Beccari T, Servillo G, Viola-Magni MP, and Orlacchio A
- Subjects
- Animals, Blotting, Northern, Brain enzymology, Epididymis enzymology, Kidney enzymology, Liver enzymology, Macromolecular Substances, Male, Mice, Mice, Inbred BALB C, RNA, Messenger biosynthesis, Spleen enzymology, Substrate Specificity, Testis enzymology, beta-N-Acetylhexosaminidases metabolism, Gene Expression, beta-N-Acetylhexosaminidases biosynthesis
- Abstract
The expression of genes encoding for the alpha and beta-subunits of the lysosomal enzyme beta-N-acetylhexosaminidase was investigated in different mouse tissues. It was found, using fluorogenic substrates, that the amounts of alpha and beta subunits were not the same in different tissues: alpha-subunit was more abundant in the brain, beta-subunit in epididymis and brain. The different isoenzyme patterns and specific activities in mouse tissues are due to the differences in the amount of hexosaminidase subunits. The mRNA, evaluated by Northern blotting analyses, revealed a greater expression of alpha-subunit in the testis and of beta-subunit in the brain and epididymis. The results indicate, therefore, that gene expression and the amount of subunits are in good relationship for beta-subunit, whereas there is no correlation for alpha-subunit.
- Published
- 1994
- Full Text
- View/download PDF
42. Age-related changes in the cell proliferation of ATPC+ mouse ascites tumour.
- Author
-
Viola-Magni MP and Biagetti M
- Subjects
- Animals, Cell Division, Mice, Mice, Inbred BALB C, Aging physiology, Ascites pathology, Cell Cycle physiology, Mammary Neoplasms, Experimental pathology
- Abstract
The growth of ATPC+, an ascites tumour derived from a spontaneous mammary carcinoma in BALB/c+ mice, was studied at different ages. It was observed that the number of cells increases rapidly during the first 5 days after implantation. Thereafter, the cell number increases more slowly, reaching a plateau after 8 days. This slowing-down is not due to a reduction in the growth fraction but to a lengthening of the cell cycle. Between 5 and 8 days the duration of all phases increases, including the S phase, which increases from 5.2 h in 5-day tumours to 8.2 h in 8-day tumours. In 12-day tumours both the cell cycle and S phase are only slightly longer than in 8-day tumours whereas the growth fraction is reduced. The slowing-down of cell growth can be attributed to growth fraction reduction rather than cell loss, which is maximal in the 5-day tumour. At this age the time course of the percent labelled cells and of the number of grains/nucleus suggests reutilization of [3H]-thymidine. Incorporation of [3H]-thymidine/cell decreases sharply in 12-day tumours due to a reduced availability of thymidine, which is degraded to thymine in the in vivo ascitic fluid faster than in 8-day tumours. This indicates an age-related change in the ascitic fluid composition.
- Published
- 1991
- Full Text
- View/download PDF
43. Hemoglobin stain in fixed erythroleukemic cells: an improvement of the method combined with autoradiography.
- Author
-
Raffa MG, Serenelli G, and Viola Magni MP
- Subjects
- Animals, Autoradiography, Benzidines, Cell Differentiation, Leukemia, Erythroblastic, Acute pathology, Mice, Hemoglobins metabolism, Leukemia, Erythroblastic, Acute metabolism, Staining and Labeling methods
- Published
- 1990
44. Base composition changes in hepatocyte nuclei DNA of rats at different ages.
- Author
-
Viola-Magni MP, Rossi R, Biondi R, and Benedetti C
- Subjects
- Aging, Animals, Animals, Newborn, Centrifugation, Density Gradient, Hydroxyapatites, Liver cytology, Liver metabolism, Methionine metabolism, Nucleic Acid Denaturation, Temperature, Thymidine metabolism, Cell Nucleus analysis, DNA metabolism, Deoxyribonucleosides analysis, Liver analysis
- Abstract
DNA extracted from isolated hepatic nuclei of rats at different aged (1 h, 6 and 30 days of life) has been characterized by (i) melting temperature, (ii) buoyant density, (iii) thermal denaturation on hydroxyapatite and (iv) nucleoside composition. The melting midpoint (Tm) determined spectrophotometrically in 0.1 X SSC (0.15 M NaCl/0.0015 M sodium citrate) is 71.9 +/- 0.4 for 1-h-old rats and decreases to 70.7 +/- 0.3 in 6-day-old animals. The buoyant densities of DNAs determined by CsCl on both native and alkaline-denaturated and reneutralized DNA were also found to decrease with age. Hydroxyapatite thermal denaturation of sonicated DNA confirmed the significant difference between the Tm values of 1-h-old and 6-day-old rats (86.5 +/- 0.5 and 85.2 +/- 0.1, respectively). The possibility that these differences in Tm values could be due to an increase in methyl bases, has been ruled out by the finding that the amount of [3H]methyl incorporated in relation to the DNA synthesis is constant at these two ages. The alternative possibility of a change in base composition has been tested by the chromatographic analysis of nucleosides. The dG + dC content is 0.433 +/- 0.003 in 1-h-old rats and decreases to 0.411 +/- 0.002 and to 0.403 +/- 0.005 in 6-day- and 30-day-old rats, respectively. The physiological significance of the different base composition is discussed in relation to the possibility that specific DNA sequences are synthesized during the non-premitotic synthesis which has been found to take place during the first 6 days of life.
- Published
- 1978
- Full Text
- View/download PDF
45. The use of (3H) thymidine-adsorbed on activated charcoal for the study of non-premitotic DNA synthesis in newborn rat hepatocytes.
- Author
-
Viola-Magni MP and Biagetti M
- Subjects
- Animals, Animals, Newborn, Cell Nucleus metabolism, Charcoal, Chromatography, Affinity, Kinetics, Liver cytology, Mitosis, Rats, Rats, Inbred Strains, Thymidine, Tritium, DNA isolation & purification, DNA Replication, Liver metabolism
- Abstract
DNA synthesis in newborn rat hepatocytes was studied in the first three days of life by means of repeated injections of (3H) thymidine. One group of animals was treated with the label adsorbed on activated charcoal (experimental group) and another group (controls) was given the label diluted in saline. The specific activity of DNA was higher in control group, but its increase was not linear with time; in the experimental group, the radioactivity was lower, but its increase with time was linear. The percentage of labeled nuclei was higher in the experimental animals than in the controls and increased linearly with time. The average number of grains/nucleus was considerably smaller in the experimental group than in the controls, in which also the percentage of labeled cells showed considerable variations during the first three days of life. It is concluded that activated charcoal adsorption increases label availability with time and, by keeping a lower label concentration in the pool, reduces the risk of radiation damage.
- Published
- 1984
46. Formation and turnover of a new aliquot of nuclear DNA [proceedings].
- Author
-
Viola-Magni MP
- Subjects
- Adrenal Glands metabolism, Animals, Kidney Tubules metabolism, Liver metabolism, Rats, Cell Nucleus metabolism, DNA metabolism
- Published
- 1976
47. [Quantitative and qualitative variations of DNA in relation to cell differentiation and function].
- Author
-
Viola-Magni MP
- Subjects
- Adrenal Glands embryology, Animals, Liver embryology, Rats, Adrenal Glands cytology, Cell Differentiation, Cell Nucleus metabolism, DNA metabolism, Liver cytology
- Published
- 1975
48. Chromatin phospholipids and DNA synthesis in hepatic cells.
- Author
-
Viola Magni MP, Gahan PB, Albi E, Iapoce R, and Gentilucci PF
- Subjects
- Animals, Cell Cycle, Cell Nucleus metabolism, DNA Replication, Female, Male, Microsomes metabolism, Rats, Time Factors, Chromatin metabolism, DNA biosynthesis, Liver Regeneration, Phospholipids metabolism
- Abstract
The synthesis of phospholipids found in microsomes, in the nuclei and in chromatin has been studied in rat liver after partial hepatectomy. [32P]O4(2-)incorporation in phospholipids has been compared with that of (3H) thymidine over a period of 48 h after operation. The presence of two peaks of DNA synthesis has been observed at 18 and 36 h; nuclear phospholipids show a continuous synthesis starting from 12 h, whereas the microsomes show two peaks at 12 and 24-30 h. The specific activity of the chromatin phospholipid fraction increases at 12h, doubles its initial value at 18 h, shows a peak at 30 h and comes back to the initial value at 48 h. It is concluded that chromatin phospholipids increase their synthesis in relation to the S phase of the cell cycle, whereas those of the nuclear membranes do not change the rate of synthesis throughout the cell cycle. The possibility is suggested that chromatin phospholipids are synthesized in the microsomes and transferred to the nucleus.
- Published
- 1985
49. Changes in endonuclease activity and in chromatin structure of rat hepatocytes during fetal and neonatal life.
- Author
-
Rossi R and Viola-Magni MP
- Subjects
- Animals, Female, Liver enzymology, Liver ultrastructure, Male, Nucleosomes analysis, Rats, Rats, Inbred Strains, Repetitive Sequences, Nucleic Acid, Chromatin metabolism, DNA metabolism, Endonucleases metabolism, Liver growth & development
- Abstract
A developmental study of rat hepatic endonuclease has been performed. Nuclei, from different stages of hepatocyte maturation, were analyzed for endogenous endonuclease activity. The chromatin extracted from these nuclei does not show any fragmentation during the first 17 days of fetal development. On the 18th day of fetal life there is a massive increase in specific endonuclease activity. At birth this activity reaches a maximum level (3.5 units/mg DNA); thereafter it undergoes a gradual decrease. The size of the basic DNA repeats produced by the endonuclease action is 218.9 +/- 1.6 in 18-day-old fetuses and decreases to 204.9 +/- 2.5 in 19-day-old fetuses, a value which remains constant in the following fetal and postnatal life. This difference in monomer size is due to changes in the chromatin structure. Micrococcal nuclease digests show that the "nucleosome core" does not change during hepatocyte development. Therefore, the difference in size of the endonuclease DNA fragments must be due to the linker regions.
- Published
- 1982
- Full Text
- View/download PDF
50. Synthesis of chromatin phospholipids.
- Author
-
Viola-Magni MP, Gahan PB, Albi E, Iapoce R, and Gentilucci PF
- Subjects
- Animals, Autoradiography, Cell Nucleus metabolism, DNA biosynthesis, Interphase, Kinetics, Liver ultrastructure, Liver Regeneration, Microsomes, Liver metabolism, Phosphates metabolism, RNA metabolism, Rats, Chromatin metabolism, Phospholipids biosynthesis
- Abstract
The presence of a phospholipid fraction associated with chromatin has been demonstrated by biochemical technique in rat hepatocytes. The composition of this fraction determined by chromatography with respect to that of the nuclei is characterized by low content of phosphatidylserine and high content in phosphatidylethanolamine. Also the synthesis and turnover studied after injection of [32P]O4(2-) show a different behaviour: the peak of activity is after 6 hrs in nuclei and microsomes, whereas in chromatin it occurs after 9 hrs. A second peak is evident after 24 hrs in chromatin and microsome phospholipids. Differences have been also shown by analyzing the single phospholipid radioactivity in time. The behaviour of chromatin phospholipids has also been studied during DNA premitotic synthesis in regenerating liver. It has been shown that there is no difference in synthesis in relation to that of DNA in nuclear phospholipids, whereas the specific activity of chromatin phospholipids begins to increase twelve hours after hepatectomy and continues throughout the period of the first mitotic wave, thus bringing to a summation with the beginning of the second wave. The role of this phospholipid fraction in relation to DNA synthesis and gene expression is discussed.
- Published
- 1987
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