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A educação inclusiva é fundamental para que crianças e adolescentes vivenciem ideias e experiências de ensino aprendizagem significativa, desenvolvam a autonomia e conquistem direitos de cidadania. No entanto, existem obstáculos que precisam ser compreendidos e superados e estratégias que podem ser adotadas para promover a inclusão de crianças com deficiência intelectual ou atraso cognitivo. Diante disso, este estudo tem como objetivo compreender o processo de inclusão escolar de alunos com deficiência intelectual ou atraso cognitivo. Para isso, trata-se de uma revisão sistemática de literatura, desenvolvida a partir da seleção de estudos nas bases de dados Scielo, Pubmed e BVS/Medline a partir do uso de descritores DeCS/MeSH e aplicação de critérios de inclusão e exclusão. Após a análise e interpretação dos dados, concluiu-se que, no processo de inclusão de alunos com deficiência intelectual ou atraso cognitivo no ambiente escolar, a educação inclusiva interfere positivamente na qualidade de vida desses. Para isso, destacam-se uma série de estratégias relevantes, tais como: envolvimento de escola como um todo, dos professores e da família; compreender a deficiência; valorizar os interesses e habilidades dos alunos com deficiência; estimular a autodeterminação desses e a convivência entre pessoas deficientes e não deficientes; promover a socialização por meio de jogos; utilizar atividades adaptadas; e cuidar da formação inicial e continuada dos professores, contemplando ideias sobre educação inclusiva.

A síndrome de Berardinelli-Seip (SBS) é uma desordem genética rara, de herança autossômica recessiva, que possui relação direta com consanguinidade paterna. Caracteriza-se por uma lipodistrofia total congênita, que acarreta atrofia completa do tecido adiposo subcutâneo e extrema resistência à insulina. Diabetes melito tipo II não cetótico e insulino resistente, hepatomegalia secundária a esteatose hepática, dislipidemia com significativa hipertrigliceridemia e hipertrofia do músculo esquelético compõe clinicamente sua apresentação. O diagnóstico de tal enfermidade é normalmente firmado até a adolescência a partir de critérios específicos, distribuídos entre critérios maiores e menores. O manejo clínico é essencialmente centrado na modulação e enfrentamento das complicações metabólicas relacionadas à síndrome, sendo o gerenciamento dietético o aspecto de maior relevância. Este estudo busca relatar o caso de uma paciente portadora de Síndrome de Berardinelli, com acometimento familiar, acompanhada no serviço de Atenção Primária no município de Patos de Minas – Minas Gerais. Trata-se de um relato de caso com revisão de literatura realizada em janeiro de 2021.

Made available in DSpace on 2021-06-25T11:18:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-09-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Rhamnolipids are glycolipid biosurfactants that have remarkable physicochemical characteristics, such as the capacity for self-assembly, which makes these biomolecules a promising option for application in nanobiotechnology. Rhamnolipids produced from a low-cost carbon source (glycerol) were used to stabilize silver nanoparticles. Silver nanoparticles (AgNPs) have been the subject of studies due to their physical chemical as well as biological properties, which corroborate their catalytic and antimicrobial activity. We compared nanoparticles obtained with three different pH values during synthesis (5, 7 and 9) in the presence of rhamnolipids. Dynamic light scattering showed that larger particles were formed at pH 5 (78–190 nm) compared to pH 7 (6.5–43 nm) and 9 (5.6–28.1 nm). Moreover, nanoparticle stability (analyzed based on the zeta potential) was enhanced with the increase in pH from 5 to 9 (−29.86 ± 1.04, −37.83 ± 0.90 and −40.33 ± 0.57 mV, respectively). Field emission gun scanning electron microscopy confirmed the round morphology of the silver nanoparticles. The LSPR spectra of AgNP for the pHs studied are conserved. In conclusion, different pH values in the presence of rhamnolipids used in the synthesis of silver nanoparticles directly affect nanoparticle size and stability. São Paulo State University (Unesp) Institute of Biosciences, Rio Claro São Paulo State University (Unesp) Institute for Research in Bioenergy, Rio Claro São Paulo State University (Unesp) Institute of Chemistry, Araraquara Federal University of São Carlos (UFSCar) Lagoa do Sino Campus, Buri São Paulo State University (Unesp) Institute of Biosciences, Rio Claro São Paulo State University (Unesp) Institute for Research in Bioenergy, Rio Claro São Paulo State University (Unesp) Institute of Chemistry, Araraquara

Beatriz Cristina Pecoraro Sanches,1 Camila Aguiar Rocha,1 Jose Gregorio Martin Bedoya,1 Vinicius Luiz da Silva,2 Patrícia Bento da Silva,3 Ana Marisa Fusco-Almeida,4 Marlus Chorilli,3 Jonas Contiero,2 Edson Crusca,1 Reinaldo Marchetto1 1São Paulo State University (UNESP), Institute of Chemistry, Department of Biochemistry and Organic Chemistry, Araraquara, SP, Brazil; 2São Paulo State University (UNESP), Institute of Biosciences, Department of General and Applied Biology, Rio Claro, SP, Brazil; 3São Paulo State University (UNESP), School of Pharmaceutical Sciences, Department of Drugs and Medicines, Araraquara, SP, Brazil; 4São Paulo State University (UNESP), School of Pharmaceutical Sciences, Department of Clinical Analysis, Araraquara, SP, BrazilCorrespondence: Reinaldo MarchettoUNESP Institute of Chemistry, Rua Prof. Francisco Degni, 55, Araraquara, 14.800-060, SP, BrazilTel +55 16 3301 9670Fax +55 16 3322 2308Email reinaldo.marchetto@unesp.brBackground: Antimicrobial resistance poses substantial risks to human health. Thus, there is an urgent need for novel antimicrobial agents, including alternative compounds, such as peptides derived from bacterial toxin-antitoxin (TA) systems. ParELC3 is a synthetic peptide derived from the ParE toxin reported to be a good inhibitor of bacterial topoisomerases and is therefore a potential antibacterial agent. However, ParELC3 is inactive against bacteria due to its inability to cross the bacterial membranes. To circumvent this limitation we prepared and used rhamnolipid-based liposomes to carry and facilitate the passage of ParELC3 through the bacterial membrane to reach its intracellular target - the topoisomerases.Methods and Results: Small unilamellar liposome vesicles were prepared by sonication from three formulations that included 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and cholesterol. ParELC3 was loaded with high efficiency into the liposomes. Characterization by DLS and TEM revealed the appropriate size, zeta potential, polydispersity index, and morphology. In vitro microbiological experiments showed that ParELC3 loaded-liposomes are more efficient (29 to 11 μmol·L− 1) compared to the free peptide (> 100 μmol·L− 1) at inhibiting the growth of standard E. coli and S. aureus strains. RL liposomes showed high hemolytic activity but when prepared with POPC and Chol this activity had a significant reduction. Independently of the formulation, the vesicles had no detectable cytotoxicity to HepG2 cells, even at the highest concentrations tested (1.3 mmol·L− 1 and 50 μmol·L− 1 for rhamnolipid and ParELC3, respectively).Conclusion: The present findings suggest the potential use of rhamnolipid-based liposomes as nanocarrier systems to enhance the bioactivity of peptides.Keywords: rhamnolipid liposomes, biosurfactants, bioactive peptides, antimicrobial activity

Made available in DSpace on 2021-06-25T11:07:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Rhamnolipids are a class of glycolipid biosurfactants with several applications, including the bioremediation of pollutant substances. However, little is known about its application in the treatment of textile effluents. Therefore, this work aims to apply a rhamnolipid-type biosurfactant to improve the sorption of the Direct Orange 2GL textile dye. The adsorption isotherms, kinetics, and thermodynamics were investigated from a novel approach. The adsorbate/adsorbent ratio was also characterized by FT-IR spectroscopy. Bioassays using Daphnia similis verified changes in toxicity after the dye underwent the proposed treatment. The adsorption dataset was better adjusted to the Langmuir model, which indicated a strong chemical interaction coming from a finite number of binding sites. The adsorption was only possible at acidic pH. Kinetic studies further demonstrated that the adsorption equilibrium is achieved in 60 min when 1.181 μg mg−1 of dye was adsorbed by the adsorbent. Thermodynamics showed that the adsorption process was proportionally influenced by higher temperatures. The thermodynamics studies also showed a spontaneous and endothermic sorption process. The FT-IR spectroscopy identified the adsorbate/adsorbent binding sites, proving that the bond between the dye and rhamnolipid occurred through the hydrogen bonds. The recovery of both dye and rhamnolipid after the adsorption was also efficient, as more than 50% of the adsorbed dye was recovered. The post-treatment bioassays using D. similis found a significant decrease in toxicity, yielding less than 30% mortality rate after adsorption. It is concluded that the rhamnolipid was an effective adsorbent and it is a viable component to treat effluents containing textile dyes. Department of General and Applied Biology Sao Paulo State University (UNESP), 24-A Avenue, 1515 Natural Sciences Center Federal University of Sao Carlos (UFSCar), SP-189, km 12 Institute for Research in Bioenergy Sao Paulo State University (UNESP), 24-A Avenue, 1515 Department of Natural Sciences Mathematics and Education Agricultural Sciences Centre Federal University of Sao Carlos (UFSCar), SP-330, km 174 Department of General and Applied Biology Sao Paulo State University (UNESP), 24-A Avenue, 1515 Institute for Research in Bioenergy Sao Paulo State University (UNESP), 24-A Avenue, 1515 CNPq: 130621/2016-1 FAPESP: 2015/1444-2 CNPq: 302935/2015-0 CAPES: 88881.132117/2016-01

The Internet of Things (IoT) has become a relevant topic of interest in Human-Computer Interaction (HCI) since it makes feasible a future computing scenario that can change the way people experiment and use technology. IoT is a new paradigm in which intelligent objects equipped with sensors, networks, and processing technologies integrate and work together to provide smart services to users. In the face of the rapid growth in the number of connected devices in an IoT scenario, the perspective of a smarter world could be actual. However, it is still too far away due to challenges related to human interaction. To help understand the issues involved in building experiences with multiple devices, we conducted interviews with ten companies exploring the characteristics and challenges surrounding the IoT project to designing IoT technologies. The results identified a set of qualitative data from companies that are developing IoT projects allowing us to discuss future directions for research. Our main contribution is related to the identification of important aspects of IoT that impact the design of user-friendly solutions. We conclude pointing to the need for IoT design that takes into account the users' particularities.

Made available in DSpace on 2018-11-26T17:42:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-11-10 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Biosurfactants are microbial metabolites with possible applications in various industrial sectors that are considered ecofriendly molecules. In recent years, some studies identified these compounds as alternatives for the elimination of vectors of tropical diseases, such as Aedes aegypti. The major bottlenecks of biosurfactant industrial production have been the use of conventional raw materials that increase production costs as well as opportunistic or pathogenic bacteria, which restrict the application of these biomolecules. The present study shows the potential of hemicellulosic sugarcane bagasse hydrolysate as a raw material for the production of a crystalline glycolipidic BS by Scheffersomyces stipitis NRRL Y-7124, which resulted in an emulsifying index (EI24) of 70 +/- 3.4% and a superficial tension of 52 +/- 2.9 mN.m(-1). Additionally, a possible new application of these compounds as biolarvicides, mainly against A. aegypti, was evaluated. At a concentration of 800 mg.L-1, the produced biosurfactant caused destruction to the larval exoskeletons 12 h after application and presented an letal concentration (LC50) of 660 mg.L-1. Thus, a new alternative for biosurfactant production using vegetal biomass as raw material within the concept of biorefineries was proposed, and the potential of the crystalline glycolipidic biosurfactant in larvicidal formulations against neglected tropical disease vectors was demonstrated. Univ Sao Paulo, Engn Sch Lorena, Dept Biotechnol, Lorena, Brazil Sao Paulo State Univ, Dept Biochem & Microbiol, Biosci Inst, Campus Rio Claro, Rio Claro, Brazil Sao Paulo State Univ, Dept Zool, Biosci Inst, Campus Rio Claro, Rio Claro, Brazil Sao Paulo State Univ, Dept Biochem & Microbiol, Biosci Inst, Campus Rio Claro, Rio Claro, Brazil Sao Paulo State Univ, Dept Zool, Biosci Inst, Campus Rio Claro, Rio Claro, Brazil FAPESP: 2015/06238-4 FAPESP: 2016/14852-7

Made available in DSpace on 2018-12-11T17:09:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01. Added 1 bitstream(s) on 2019-10-09T18:30:48Z : No. of bitstreams: 1 S0101-20612016000300456.pdf: 765352 bytes, checksum: f0f51c7eb3ab9316c383fa7b1dc8cbca (MD5) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Microorganisms are the primary responsible for food poisoning and food spoilage. The purpose of this study was to evaluate different fruit washing methods with tap water, electrolyzed water and rhamnolipids solution produced by Pseudomonas aeruginosa LBI, in order to inhibit microbial growth. The tested organism was Eugenia uniflora. The fruits were washed and periodically inoculated into culture media to evaluate and count the colonies on the fruit surface. It was also observed the deterioration level of the fruits after each treatment. The results showed that treatment with rhamnolipids were the most efficient, inhibiting the growth of fungi and bacteria. The electrolyzed water proved to be very efficient in bacterial inhibition at the initial time, but in the final time it did not present any inhibitory effect. The electrolyzed water was also not effective in eliminating fungus. Washing with tap water was the less efficient treatment of all. The only treatment that showed an increased durability has been with rhamnolipids, increasing shelf life by up to two days. Thus rhamnolipids are the most recommended method for fruits sanitation. Department of Biochemistry and Microbiology Universidade Estadual Paulista – UNESP Department of Biochemistry and Microbiology Universidade Estadual Paulista – UNESP

Made available in DSpace on 2015-10-21T13:12:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-02-16. Added 1 bitstream(s) on 2015-10-22T09:52:02Z : No. of bitstreams: 1 WOS000350249200001.pdf: 1802706 bytes, checksum: 50173669531b4bbb58b53e8b6acbd407 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Aedes aegypti mosquitoes are the primary transmitters of dengue fever, urban yellow fever, and chikungunya viruses. This mosquito has developed resistance to the insecticides currently used to control their populations. These chemical insecticides are harmful to the environment and can have negative effects on human health. Rhamnolipids are environmentally compatible biological surfactants, but their insecticidal activity has not been extensively studied. The present study evaluated the potential larvicidal, insecticidal, and repellent activities of rhamnolipids against A. aegypti. At concentrations of 800, 900, and 1000 mg/L, rhamnolipids eliminated all mosquito larvae in 18 h and killed 100% of adults at 1000 mg/L. According to the results it may be conclude that rhamnolipids should be applied to control larvae and mosquitos besides present the repellency activity against A. aegypti. Biochemistry and Microbiology, Universidade Estadual Paulista Júlio de Mesquita Filho, Rio Claro, Brazil Zoology and Entomology, Universidade Estadual Paulista Júlio de Mesquita Filho, Rio Claro, Brazil FAPESP: 2011/15345-8

This paper evaluates the effect of additives (NaCl and ethanol) on the solution properties of rhamnolipids. The properties are the surface activity, aggregate formations and emulsifying activity as well as the synergistic effects of additives and pH variations on the physical properties of rhamnolipids. Additionally, analysis of fatty acids and rhamnolipid homologues produced using different carbon sources was performed by mass spectrometry. The results indicate that this biosurfactant maintain its properties in the presence of additives. NaCl decreases the size and number of aggregates formed in solutions without pH control, while ethanol to rhamnolipid solutions reduces critical micelle concentration and favors aggregation of monomers. The profiles of fatty acids produced by P. aeruginosa LBI 2A1 varied according to the carbon source used, however for rhamnolipids there was no difference.