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3. Hacia una teología católica del desarrollo

Author

Fabio Villegas V.

Subjects
The Bible, BS1-2970, Practical Theology, BV1-5099, Doctrinal Theology, BT10-1480
Abstract

El gran desarrollo del mundo en estos dos siglos ha sido obra principalmente de los países protestantes que componen al mismo tiempo el llamado "Norte". Ha sido un gran desarrollo sustentado por "la religión idealista del protestantismo humanista (que) se concibe en favor de la burguesía victoriosa". (Tillich, Paul: La Era protestante, pp. 254). "Aquellos que más han exaltado a menudo con un sentido de distorsión determinista la dependencia incondicional con respecto a lo divino Calvino y los puritanos crearon el tipo más activista de hombres de toda la historia" (Oc. pp. 258). "El protestantismo exige un laicismo absoluto" (Oc. 260). "Los problemas de la actividad humana no entran en el radio de acción religioso" (Oc. 263). El protestantismo calvinista se orientó particularmente en dirección a una "exaltación religiosa" (Cfr. Oc. 265).

Published
2020

7. Disentangling Signatures of Selection Before and After European Colonization in Latin Americans

Author

Kim, Y, Mendoza-Revilla, J, Chacon-Duque, JC, Fuentes-Guajardo, M, Ormond, L, Wang, K, Hurtado, M, Villegas, V, Granja, V, Acuna-Alonzo, V, Jaramillo, C, Arias, W, Barquera, R, Gomez-Valdes, J, Villamil-Ramirez, H, de Cerqueira, CCS, Rivera, KMB, Nieves-Colon, MA, Gignoux, CR, Wojcik, GL, Moreno-Estrada, A, Hunemeier, T, Ramallo, V, Schuler-Faccini, L, Gonzalez-Jose, R, Bortolini, M-C, Canizales-Quinteros, S, Gallo, C, Poletti, G, Bedoya, G, Rothhammer, F, Balding, D, Fumagalli, M, Adhikari, K, Ruiz-Linares, A, Hellenthal, G, Kim, Y, Mendoza-Revilla, J, Chacon-Duque, JC, Fuentes-Guajardo, M, Ormond, L, Wang, K, Hurtado, M, Villegas, V, Granja, V, Acuna-Alonzo, V, Jaramillo, C, Arias, W, Barquera, R, Gomez-Valdes, J, Villamil-Ramirez, H, de Cerqueira, CCS, Rivera, KMB, Nieves-Colon, MA, Gignoux, CR, Wojcik, GL, Moreno-Estrada, A, Hunemeier, T, Ramallo, V, Schuler-Faccini, L, Gonzalez-Jose, R, Bortolini, M-C, Canizales-Quinteros, S, Gallo, C, Poletti, G, Bedoya, G, Rothhammer, F, Balding, D, Fumagalli, M, Adhikari, K, Ruiz-Linares, A, and Hellenthal, G

Abstract

Throughout human evolutionary history, large-scale migrations have led to intermixing (i.e., admixture) between previously separated human groups. Although classical and recent work have shown that studying admixture can yield novel historical insights, the extent to which this process contributed to adaptation remains underexplored. Here, we introduce a novel statistical model, specific to admixed populations, that identifies loci under selection while determining whether the selection likely occurred post-admixture or prior to admixture in one of the ancestral source populations. Through extensive simulations, we show that this method is able to detect selection, even in recently formed admixed populations, and to accurately differentiate between selection occurring in the ancestral or admixed population. We apply this method to genome-wide SNP data of ∼4,000 individuals in five admixed Latin American cohorts from Brazil, Chile, Colombia, Mexico, and Peru. Our approach replicates previous reports of selection in the human leukocyte antigen region that are consistent with selection post-admixture. We also report novel signals of selection in genomic regions spanning 47 genes, reinforcing many of these signals with an alternative, commonly used local-ancestry-inference approach. These signals include several genes involved in immunity, which may reflect responses to endemic pathogens of the Americas and to the challenge of infectious disease brought by European contact. In addition, some of the strongest signals inferred to be under selection in the Native American ancestral groups of modern Latin Americans overlap with genes implicated in energy metabolism phenotypes, plausibly reflecting adaptations to novel dietary sources available in the Americas.

Published
2022

8. Hear us! Accounts of people treated with injectables for drug-resistant TB

Author

Almeida, A., primary, Adjuntsov, M., additional, Bushura, W., additional, Delgado, E., additional, Drasher, M., additional, Fernando-Pancho, M., additional, Gasane, M., additional, Ianoşi, M. V., additional, Lessem, E., additional, Musah, A., additional, Răduţ, Ş, additional, Sánchez Ríos, C. H., additional, Soe, K. S., additional, Venkatesan, N., additional, Villegas, V. V., additional, and Stillo, J., additional

Published
2021
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9. RÁPIDA Y PERTINENTE BÚSQUEDA POR INTERNET MEDIANTE OPERADORES BOOLEANOS

Author

Bayardo Villegas V.

Subjects
Búsqueda, conjunto, operador booleano, proposición, proposición funcional, Science (General), Q1-390
Abstract

Internet es una excelente y moderna herramienta para buscar información acerca de un problema particular, es como tener acceso simultáneo a muchas bibliotecas del mundo. En cualquier investigación generalmente se consigue una gran cantidad de información y mucha de ella no está relacionada con el problema de interés; el uso adecuado de los operadores booleanos produce mejores resultados en la búsqueda de información. En este escrito, la teoría básica de proposiciones y conjuntos y sus aplicaciones a la búsqueda de información en internet se discute y se ilustra con varios ejemplos.

Published
2003

10. A GWAS identifies novel gene associations with facial skin wrinkling and mole count in Latin‐Americans

Author

Chen, Y., André, M., Adhikari, K., Blin, M., Bonfante, B., Mendoza‐Revilla, J., Fuentes‐Guajardo, M., Palmal, S., Chacón‐Duque, J.C., Hurtado, M., Villegas, V., Granja, V., Jaramillo, C., Arias, W., Lozano, R.B., Everardo‐Martínez, P., Gómez‐Valdés, J., Villamil‐Ramírez, H., de Cerqueira, C.C.S., Hünemeier, T., Ramallo, V., Gonzalez‐José, R., Schüler‐Faccini, L., Bortolini, M.‐C., Acuña‐Alonzo, V., Canizales‐Quinteros, S., Gallo, C., Poletti, G., Bedoya, G., Rothhammer, F., Balding, D., Tobin, D.J., Wang, S., Faux, P., Ruiz‐Linares, A., Anthropologie bio-culturelle, Droit, Ethique et Santé (ADES), and Aix Marseille Université (AMU)-EFS ALPES MEDITERRANEE-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SHS.ANTHRO-BIO]Humanities and Social Sciences/Biological anthropology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

11. HIPOTÉTICA ETAPA PREBIÓTICA

Author

Bayardo Villegas V.

Subjects
Chaperona, Hsp, prepión, prion, proteína de estrés térmico, PrP, PrPs', [PSI+], ribozima, Sup35, Ure2, [Ure3], viroide, Science (General), Q1-390
Abstract

Comúnmente se cree que la vida se inició en épocas prebióticas con ribozimas: como solución a que las agresivas condiciones ambientales de tales épocas se opondrían a la autorreplicación de los ribozimas se plantea la hipótesis, basada en la resistencia de los priones a condiciones de ese tipo, "en algún momento prebiótico surgió una asociación ribozimalprion de tipo +1+". Para respaldarla se muestran argumentos y bibliografía.

Published
2000

12. A genome‐wide association study identifies novel gene associations with facial skin wrinkling and mole count in Latin Americans

Author

Chen, Y., primary, André, M., additional, Adhikari, K., additional, Blin, M., additional, Bonfante, B., additional, Mendoza‐Revilla, J., additional, Fuentes‐Guajardo, M., additional, Palmal, S., additional, Chacón‐Duque, J.C., additional, Hurtado, M., additional, Villegas, V., additional, Granja, V., additional, Jaramillo, C., additional, Arias, W., additional, Lozano, R.B., additional, Everardo‐Martínez, P., additional, Gómez‐Valdés, J., additional, Villamil‐Ramírez, H., additional, Cerqueira, C.C.S., additional, Hünemeier, T., additional, Ramallo, V., additional, Gonzalez‐José, R., additional, Schüler‐Faccini, L., additional, Bortolini, M.‐C., additional, Acuña‐Alonzo, V., additional, Canizales‐Quinteros, S., additional, Gallo, C., additional, Poletti, G., additional, Bedoya, G., additional, Rothhammer, F., additional, Balding, D., additional, Tobin, D.J., additional, Wang, S., additional, Faux, P., additional, and Ruiz‐Linares, A., additional

Published
2021
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13. Screening Anti-Inflammatory Effects of Flavanones Solutions

Author

Bustos-Salgado P, Andrade-Carrera B, Domínguez-Villegas V, Díaz-Garrido N, Rodríguez-Lagunas MJ, Badia J, Baldoma L, Mallandrich M, Calpena-Campmany A, and Garduño-Ramírez ML

Subjects
Eysenhardtia platycarpa, flavanones, in vivo anti-inflammatory activity, cytokines
Abstract

There are a large number of remedies in traditional medicine focused on relieving pain and inflammation. Flavanones have been a potential source in the search for leading compounds and biologically active components, and they have been the focus of much research and development in recent years. Eysenhardtia platycarpa is used in traditional medicine for the treatment of kidney diseases, bladder infections, and diabetes mellitus. Many compounds have been isolated from this plant, such as flavones, flavanones, phenolic compounds, triterpenoid acids, chalcones, sugars, and fatty acids, among others. In this paper, natural flavanone 1 (extracted from Eysenhardtia platycarpa) as lead compound and flavanones 1a-1d as its structural analogues were screened for anti-inflammatory activity using Molinspiration(®) and PASS Online in a computational study. The hydro alcoholic solutions (FS) of flavanones 1, 1a-1d (FS1, FS1a-FS1d) were also assayed to investigate their in vivo anti-inflammatory cutaneous effect using two experimental models, a rat ear edema induced by arachidonic acid (AA) and a mouse ear edema induced by 12-O-tetradecanoylphorbol acetate (TPA). Histological studies and analysis of pro-inflammatory cytokines TNF-a, IL-1ß, and IL-6 were also assessed in AA-inflamed rat ear tissue. The results showed that the flavanone hydro alcoholic solutions (FS) caused edema inhibition in both evaluated models. This study suggests that the evaluated flavanones will be effective when used in the future in skin pathologies with inflammation, with the results showing 1b and 1d to be the best.

Published
2021

14. A GWAS in Latin Americans identifies novel face shape loci, implicating VPS13B and a Denisovan introgressed region in facial variation

Author

Bonfante, B. (Betty), Faux, P. (Pierre), Navarro, N. (Nicolas), Mendoza-Revilla, J. (Javier), Dubied, M. (Morgane), Montillot, C. (Charlotte), Wentworth, E. (Emma), Poloni, L. (Lauriane), Varón-González, C. (Ceferino), Jones, P. (Philip), Xiong, Z. (Ziyi), Fuentes-Guajardo, M. (Macarena), Palmal, S. (Sagnik), Chacón-Duque, J.C. (Juan Camilo), Hurtado, M. (Malena), Villegas, V. (Valeria), Granja, V. (Vanessa), Jaramillo, C. (Claudia), Arias, W. (William), Barquera, R. (Rodrigo), Everardo-Martínez, P. (Paola), Sánchez-Quinto, M. (Mirsha), Gómez-Valdés, J. (Jorge), Villamil-Ramírez, H. (Hugo), Silva de Cerqueira, C.C. (Caio C.), Hünemeier, T. (Tábita), Ramallo, V. (Virginia), Liu, F. (Fan), Weinberg, S.M. (Seth M.), Shaffer, J.R. (John R), Stergiakouli, E. (Evie), Howe, L.J. (Laurence J.), Hysi, P.G. (Pirro G.), Spector, T.D. (Timothy D.), Gonzalez-José, R. (Rolando), Schüler-Faccini, L. (Lavinia), Bortolini, M.-C. (Maria-Cátira), Acuña-Alonzo, V. (Victor), Canizales-Quinteros, S. (Samuel), Gallo, C. (Carla), Poletti, G. (Giovanni), Bedoya, E.G. (Elsie), Rothhammer, F. (Francisco), Thauvin-Robinet, C. (Christel), Faivre, L. (Laurence), Costedoat, C. (Caroline), Balding, D.J. (David), Cox, T. (Timothy), Kayser, M.H. (Manfred), Duplomb, L. (Laurence), Yalcin, B. (Binnaz), Cotney, J. (Justin), Adhikari, K. (Kaustubh), Ruiz-Linares, A. (Andres), Bonfante, B. (Betty), Faux, P. (Pierre), Navarro, N. (Nicolas), Mendoza-Revilla, J. (Javier), Dubied, M. (Morgane), Montillot, C. (Charlotte), Wentworth, E. (Emma), Poloni, L. (Lauriane), Varón-González, C. (Ceferino), Jones, P. (Philip), Xiong, Z. (Ziyi), Fuentes-Guajardo, M. (Macarena), Palmal, S. (Sagnik), Chacón-Duque, J.C. (Juan Camilo), Hurtado, M. (Malena), Villegas, V. (Valeria), Granja, V. (Vanessa), Jaramillo, C. (Claudia), Arias, W. (William), Barquera, R. (Rodrigo), Everardo-Martínez, P. (Paola), Sánchez-Quinto, M. (Mirsha), Gómez-Valdés, J. (Jorge), Villamil-Ramírez, H. (Hugo), Silva de Cerqueira, C.C. (Caio C.), Hünemeier, T. (Tábita), Ramallo, V. (Virginia), Liu, F. (Fan), Weinberg, S.M. (Seth M.), Shaffer, J.R. (John R), Stergiakouli, E. (Evie), Howe, L.J. (Laurence J.), Hysi, P.G. (Pirro G.), Spector, T.D. (Timothy D.), Gonzalez-José, R. (Rolando), Schüler-Faccini, L. (Lavinia), Bortolini, M.-C. (Maria-Cátira), Acuña-Alonzo, V. (Victor), Canizales-Quinteros, S. (Samuel), Gallo, C. (Carla), Poletti, G. (Giovanni), Bedoya, E.G. (Elsie), Rothhammer, F. (Francisco), Thauvin-Robinet, C. (Christel), Faivre, L. (Laurence), Costedoat, C. (Caroline), Balding, D.J. (David), Cox, T. (Timothy), Kayser, M.H. (Manfred), Duplomb, L. (Laurence), Yalcin, B. (Binnaz), Cotney, J. (Justin), Adhikari, K. (Kaustubh), and Ruiz-Linares, A. (Andres)

Abstract

To characterize the genetic basis of facial features in Latin Americans, we performed a genome-wide association study (GWAS) of more than 6000 individuals using 59 landmark-based measurements from two-dimensional profile photographs and ~9,000,000 genotyped or imputed single-nucleotide polymorphisms. We detected significant association of 32 traits with at least 1 (and up to 6) of 32 different genomic regions, more than doubling the number of robustly associated face morphology loci reported until now (from 11 to 23). These GWAS hits are strongly enriched in regulatory sequences active specifically during craniofacial development. The associated region in 1p12 includes a tract of archaic adaptive introgression, with a Denisovan haplotype common in Native Americans affecting particularly lip thickness. Among the nine previously unidentified face morphology loci we identified is the VPS13B gene region, and we show that variants in this region also affect midfacial morphology in mice.

Published
2021
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15. Prediction of eye, hair and skin colour in Latin Americans

Author

Palmal, S, Adhikari, K, Mendoza-Revilla, J, Fuentes-Guajardo, M, de Cerqueira, CCS, Bonfante, B, Chacon-Duque, JC, Sohail, A, Hurtado, M, Villegas, V, Granja, V, Jaramillo, C, Arias, W, Barquera Lozano, R, Everardo-Martinez, P, Gomez-Valdes, J, Villamil-Ramirez, H, Hunemeier, T, Ramallo, V, Parolin, M-L, Gonzalez-Jose, R, Schuler-Faccini, L, Bortolini, M-C, Acuna-Alonzo, V, Canizales-Quinteros, S, Gallo, C, Poletti, G, Bedoya, G, Rothhammer, F, Balding, D, Faux, P, Ruiz-Linares, A, Palmal, S, Adhikari, K, Mendoza-Revilla, J, Fuentes-Guajardo, M, de Cerqueira, CCS, Bonfante, B, Chacon-Duque, JC, Sohail, A, Hurtado, M, Villegas, V, Granja, V, Jaramillo, C, Arias, W, Barquera Lozano, R, Everardo-Martinez, P, Gomez-Valdes, J, Villamil-Ramirez, H, Hunemeier, T, Ramallo, V, Parolin, M-L, Gonzalez-Jose, R, Schuler-Faccini, L, Bortolini, M-C, Acuna-Alonzo, V, Canizales-Quinteros, S, Gallo, C, Poletti, G, Bedoya, G, Rothhammer, F, Balding, D, Faux, P, and Ruiz-Linares, A

Abstract

Here we evaluate the accuracy of prediction for eye, hair and skin pigmentation in a dataset of > 6500 individuals from Mexico, Colombia, Peru, Chile and Brazil (including genome-wide SNP data and quantitative/categorical pigmentation phenotypes - the CANDELA dataset CAN). We evaluated accuracy in relation to different analytical methods and various phenotypic predictors. As expected from statistical principles, we observe that quantitative traits are more sensitive to changes in the prediction models than categorical traits. We find that Random Forest or Linear Regression are generally the best performing methods. We also compare the prediction accuracy of SNP sets defined in the CAN dataset (including 56, 101 and 120 SNPs for eye, hair and skin colour prediction, respectively) to the well-established HIrisPlex-S SNP set (including 6, 22 and 36 SNPs for eye, hair and skin colour prediction respectively). When training prediction models on the CAN data, we observe remarkably similar performances for HIrisPlex-S and the larger CAN SNP sets for the prediction of hair (categorical) and eye (both categorical and quantitative), while the CAN sets outperform HIrisPlex-S for quantitative, but not for categorical skin pigmentation prediction. The performance of HIrisPlex-S, when models are trained in a world-wide sample (although consisting of 80% Europeans, https://hirisplex.erasmusmc.nl), is lower relative to training in the CAN data (particularly for hair and skin colour). Altogether, our observations are consistent with common variation of eye and hair colour having a relatively simple genetic architecture, which is well captured by HIrisPlex-S, even in admixed Latin Americans (with partial European ancestry). By contrast, since skin pigmentation is a more polygenic trait, accuracy is more sensitive to prediction SNP set size, although here this effect was only apparent for a quantitative measure of skin pigmentation. Our results support the use of HIrisPlex-S in the pr

Published
2021

16. A GWAS in Latin Americans identifies novel face shape loci, implicating VPS13B and a Denisovan introgressed region in facial variation

Author

Bonfante, B, Faux, P, Navarro, N, Mendoza-Revilla, J, Dubied, M, Montillot, C, Wentworth, E, Poloni, L, Varon-Gonzalez, C, Jones, P, Xiong, Z, Fuentes-Guajardo, M, Palmal, S, Chacon-Duque, JC, Hurtado, M, Villegas, V, Granja, V, Jaramillo, C, Arias, W, Barquera, R, Everardo-Martinez, P, Sanchez-Quinto, M, Gomez-Valdes, J, Villamil-Ramirez, H, de Cerqueira, CCS, Hunemeier, T, Ramallo, V, Liu, F, Weinber, SM, Shaffer, JR, Stergiakouli, E, Howe, LJ, Hysi, PG, Spector, TD, Gonzalez-Jose, R, Schuler-Faccini, L, Bortolini, R-C, Acuna-Alonzo, V, Canizales-Quinteros, S, Gallo, C, Poletti, G, Bedoya, G, Rothhammer, F, Thauvin-Robinet, C, Faivre, L, Costedoat, C, Balding, D, Cox, T, Kayser, M, Duplomb, L, Yalcin, B, Cotney, J, Adhikari, K, Ruiz-Linares, A, Bonfante, B, Faux, P, Navarro, N, Mendoza-Revilla, J, Dubied, M, Montillot, C, Wentworth, E, Poloni, L, Varon-Gonzalez, C, Jones, P, Xiong, Z, Fuentes-Guajardo, M, Palmal, S, Chacon-Duque, JC, Hurtado, M, Villegas, V, Granja, V, Jaramillo, C, Arias, W, Barquera, R, Everardo-Martinez, P, Sanchez-Quinto, M, Gomez-Valdes, J, Villamil-Ramirez, H, de Cerqueira, CCS, Hunemeier, T, Ramallo, V, Liu, F, Weinber, SM, Shaffer, JR, Stergiakouli, E, Howe, LJ, Hysi, PG, Spector, TD, Gonzalez-Jose, R, Schuler-Faccini, L, Bortolini, R-C, Acuna-Alonzo, V, Canizales-Quinteros, S, Gallo, C, Poletti, G, Bedoya, G, Rothhammer, F, Thauvin-Robinet, C, Faivre, L, Costedoat, C, Balding, D, Cox, T, Kayser, M, Duplomb, L, Yalcin, B, Cotney, J, Adhikari, K, and Ruiz-Linares, A

Abstract

To characterize the genetic basis of facial features in Latin Americans, we performed a genome-wide association study (GWAS) of more than 6000 individuals using 59 landmark-based measurements from two-dimensional profile photographs and ~9,000,000 genotyped or imputed single-nucleotide polymorphisms. We detected significant association of 32 traits with at least 1 (and up to 6) of 32 different genomic regions, more than doubling the number of robustly associated face morphology loci reported until now (from 11 to 23). These GWAS hits are strongly enriched in regulatory sequences active specifically during craniofacial development. The associated region in 1p12 includes a tract of archaic adaptive introgression, with a Denisovan haplotype common in Native Americans affecting particularly lip thickness. Among the nine previously unidentified face morphology loci we identified is the VPS13B gene region, and we show that variants in this region also affect midfacial morphology in mice.

Published
2021

17. A genome-wide association study identifies novel gene associations with facial skin wrinkling and mole count in Latin Americans

Author

Chen, Y, Andre, M, Adhikari, K, Blin, M, Bonfante, B, Mendoza-Revilla, J, Fuentes-Guajardo, M, Palmal, S, Chacon-Duque, JC, Hurtado, M, Villegas, V, Granja, V, Jaramillo, C, Arias, W, Lozano, RB, Everardo-Martinez, P, Gomez-Valdes, J, Villamil-Ramirez, H, de Cerqueira, CCS, Hunemeier, T, Ramallo, V, Gonzalez-Jose, R, Schuler-Faccini, L, Bortolini, M-C, Acuna-Alonzo, V, Canizales-Quinteros, S, Gallo, C, Poletti, G, Bedoya, G, Rothhammer, F, Balding, D, Tobin, DJ, Wang, S, Faux, P, Ruiz-Linares, A, Chen, Y, Andre, M, Adhikari, K, Blin, M, Bonfante, B, Mendoza-Revilla, J, Fuentes-Guajardo, M, Palmal, S, Chacon-Duque, JC, Hurtado, M, Villegas, V, Granja, V, Jaramillo, C, Arias, W, Lozano, RB, Everardo-Martinez, P, Gomez-Valdes, J, Villamil-Ramirez, H, de Cerqueira, CCS, Hunemeier, T, Ramallo, V, Gonzalez-Jose, R, Schuler-Faccini, L, Bortolini, M-C, Acuna-Alonzo, V, Canizales-Quinteros, S, Gallo, C, Poletti, G, Bedoya, G, Rothhammer, F, Balding, D, Tobin, DJ, Wang, S, Faux, P, and Ruiz-Linares, A

Abstract

BACKGROUND: Genome-wide association studies (GWASs) have identified genes influencing skin ageing and mole count in Europeans, but little is known about the relevance of these (or other genes) in non-Europeans. OBJECTIVES: To conduct a GWAS for facial skin ageing and mole count in adults < 40 years old, of mixed European, Native American and African ancestry, recruited in Latin America. METHODS: Skin ageing and mole count scores were obtained from facial photographs of over 6000 individuals. After quality control checks, three wrinkling traits and mole count were retained for genetic analyses. DNA samples were genotyped with Illumina's HumanOmniExpress chip. Association testing was performed on around 8 703 729 single-nucleotide polymorphisms (SNPs) across the autosomal genome. RESULTS: Genome-wide significant association was observed at four genome regions: two were associated with wrinkling (in 1p13·3 and 21q21·2), one with mole count (in 1q32·3) and one with both wrinkling and mole count (in 5p13·2). Associated SNPs in 5p13·2 and in 1p13·3 are intronic within SLC45A2 and VAV3, respectively, while SNPs in 1q32·3 are near the SLC30A1 gene, and those in 21q21·2 occur in a gene desert. Analyses of SNPs in IRF4 and MC1R are consistent with a role of these genes in skin ageing. CONCLUSIONS: We replicate the association of wrinkling with variants in SLC45A2, IRF4 and MC1R reported in Europeans. We identify VAV3 and SLC30A1 as two novel candidate genes impacting on wrinkling and mole count, respectively. We provide the first evidence that SLC45A2 influences mole count, in addition to variants in this gene affecting melanoma risk in Europeans.

Published
2021

18. Prospecting for lytic bacteriophages against diverse strains of Ralstonia solanacearum from Musa spp. in Colombia.

Author

Universidad EAFIT. Departamento de Ciencias, Ciencias Biológicas y Bioprocesos (CIBIOP), Villegas, V., Álvarez, J.C., Universidad EAFIT. Departamento de Ciencias, Ciencias Biológicas y Bioprocesos (CIBIOP), Villegas, V., and Álvarez, J.C.

Abstract

Bacterial wilt caused by the soilborne pathogen Ralstonia solanacearum is one of the most devastating plant diseases worldwide. In Colombia, this disease causes high economic losses in plantations of banana

Published
2021

20. Preoperative Bevacizumab for Tractional Retinal Detachment in Proliferative Diabetic Retinopathy: A Prospective Randomized Clinical Trial

Author

Arevalo, JF, Lasave, AF, Kozak, I, Al Rashaed, S, Al Kahtani, E, Maia, M, Farah, ME, Cutolo, C, Brito, M, Osorio, C, Navarro, P, Wu, L, Berrocal, MH, Morales-Canton, V, Serrano, MA, Graue-Wiechers, F, Sabrosa, NA, Alezzandrini, AA, Gallego-Pinazo, R, Liu, TYA, Farah, M, Penha, FM, Rodrigues, EB, Fromow-Guerra, J, Naranjo, JLG, Dalma-Weiszhausz, J, Velez-Montoya, R, Quiroz-Mercado, H, Rodriguez, FJ, Gomez, FE, Brieke, AC, Goveto, A, Cruz-Villegas, V, Lozano-Rechy, D, Fulda-Graue, E, Roca, JA, Hernandez, A, Saravia, MJ, Schlaen, A, Rojas, J, Lngolotti, M, Avila, M, Carla, L, Cardillo, J, Jorge, R, Carpentier, C, Verdaguer, J, Verdaguer, JI, Sepulveda, G, Alezzandrini, A, Garcia, B, Zas, M, Diaz-Llopis, M, Dolz-Marco, R, Figueroa, M, Contreras, I, Ruiz-Casas, D, and Pan Amer Collaborative Retina

Subjects
genetic structures, sense organs, eye diseases
Abstract

PURPOSE: To assess the effectiveness and safety of an intravitreal injection of 1.25 mg bevacizumab (IVB) as a preoperative adjunct to small-gauge pars plana vitrectomy (PPV) compared with PPV alone in eyes with tractional retinal detachment secondary to proliferative diabetic retinopathy. METHODS: This prospective, double-masked, randomized, multicenter, active-controlled clinical trial enrolled 224 eyes of 224 patients between November 2013 and July 2015. All eyes underwent a baseline examination including best-corrected visual acuity, color photos, optical coherence tomography, and fluorescein angiography. Data were collected on intraoperative bleeding, total surgical time, early (< 1 month) postoperative vitreous hemorrhage, and mean change in best-corrected visual acuity at 12 months. P < .05 was considered statistically significant. RESULTS: A total of 214 patients (214 eyes) were randomized in a 1:1 ratio to PPV plus IVB ([study group] 102 eyes) or PPV plus sham ([control] 112 eyes). Iatrogenic retinal breaks were noted intraoperatively in 35 eyes (34.3%) in the study group, and 66 eyes (58.9%) in the control group (P = .001). Grade 2 intraoperative bleeding was noted in 32 (31.3%) eyes in the study group and 58 (51.7 %) eyes in the control group (P = .001). Endodiathermy was necessary in 28 (27.4 %) eyes in the study group, compared with 75 (66.9%) eyes in the control group (P = .0001). Mean surgical time was 71.3 +/- 32.1 minutes in the study group and 83.6 +/- 38.7 minutes in the control group (P = .061). CONCLUSION: Preoperative IVB seems to reduce intraoperative bleeding, improving surgical field visualization, and reducing intraoperative and postoperative complications. (C) 2019 Elsevier Inc. All rights reserved.

Published
2019

21. A GWAS in Latin Americans highlights the convergent evolution of lighter skin pigmentation in Eurasia

Author

Adhikari, K, Mendoza-Revilla, J, Sohail, A, Fuentes-Guajardo, M, Lampert, J, Chacon-Duque, JC, Hurtado, M, Villegas, V, Granja, V, Acuna-Alonzo, V, Jaramillo, C, Arias, W, Barquera Lozano, R, Everardo, P, Gomez-Valdes, J, Villamil-Ramirez, H, Silva de Cerqueira, CC, Hunemeier, T, Ramallo, V, Schuler-Faccini, L, Salzano, FM, Gonzalez-Jose, R, Bortolini, M-C, Canizales-Quinteros, S, Gallo, C, Poletti, G, Bedoya, G, Rothhammer, F, Tobin, DJ, Fumagalli, M, Balding, D, Ruiz-Linares, A, Adhikari, K, Mendoza-Revilla, J, Sohail, A, Fuentes-Guajardo, M, Lampert, J, Chacon-Duque, JC, Hurtado, M, Villegas, V, Granja, V, Acuna-Alonzo, V, Jaramillo, C, Arias, W, Barquera Lozano, R, Everardo, P, Gomez-Valdes, J, Villamil-Ramirez, H, Silva de Cerqueira, CC, Hunemeier, T, Ramallo, V, Schuler-Faccini, L, Salzano, FM, Gonzalez-Jose, R, Bortolini, M-C, Canizales-Quinteros, S, Gallo, C, Poletti, G, Bedoya, G, Rothhammer, F, Tobin, DJ, Fumagalli, M, Balding, D, and Ruiz-Linares, A

Abstract

We report a genome-wide association scan in >6,000 Latin Americans for pigmentation of skin and eyes. We found eighteen signals of association at twelve genomic regions. These include one novel locus for skin pigmentation (in 10q26) and three novel loci for eye pigmentation (in 1q32, 20q13 and 22q12). We demonstrate the presence of multiple independent signals of association in the 11q14 and 15q13 regions (comprising the GRM5/TYR and HERC2/OCA2 genes, respectively) and several epistatic interactions among independently associated alleles. Strongest association with skin pigmentation at 19p13 was observed for an Y182H missense variant (common only in East Asians and Native Americans) in MFSD12, a gene recently associated with skin pigmentation in Africans. We show that the frequency of the derived allele at Y182H is significantly correlated with lower solar radiation intensity in East Asia and infer that MFSD12 was under selection in East Asians, probably after their split from Europeans.

Published
2019

22. Evidence for a two-state transition in the folding process of the activation domain of human procarboxypeptidase A2

Author

Villegas, V., Azuaga, A., Catasus, Ll., Reverter, D., Mateo, P.L., Aviles, F.X., and Serrano, L.

Subjects
Protein folding -- Research, Biological sciences, Chemistry
Abstract

The thermodynamic and kinetic behavior of human procarboxypeptidase A2 (ADA2h) is determined. ADA2h is a globular open-sandwich alpha + beta domain with 80 residues and no disulfide bridges. The results showed that equilibrium denaturation bu urea or temperature is reversible at pH 7.0 and fits to a two-state transition. Kinetic studies of unfolding and refolding did not indicate any kinetic intermediate accumulating in the folding reaction.

Published
1995

23. Latin Americans show wide-spread Converso ancestry and the imprint of local Native ancestry on physical appearance

Author

Chacon-Duque, J., Adhikari, K., Fuentes-Guajardo, M., Mendoza-Revilla, J., Acuna-Alonzo, V., Barquera Lozano, R., Quinto-Sanchez, M., Gomez-Valdes, J., Everardo Martinez, P., Villamil-Ramirez, H., Hunemeier, T., Ramallo, V., Silva de Cerqueira, C., Hurtado, M., Villegas, V., Granja, V., Villena, M., Vasquez, R., Llop, E., Sandoval, J., Salazar-Granara, A., Parolin, M., Sandoval, K., Penaloza-Espinosa, R., Rangel-Villalobos, H., Winkler, C., Klitz, W., Bravi, C., Molina, J., Corach, D., Barrantes, R., Gomes, V., Resende, C., Gusmao, L., Amorim, A., Xue, Y., Dugoujon, J., Moral, P., Gonzalez-Jose, R., Schuler-Faccini, L., Salzano, F., Bortolini, M., Canizales-Quinteros, S., Poletti, G., Gallo, C., Bedoya, G., Rothhammer, F., Balding, D., Hellenthal, G., and Ruiz-Linares, A.

Subjects
parasitic diseases
Abstract

Historical records and genetic analyses indicate that Latin Americans trace their ancestry mainly to the admixture of Native Americans, Europeans and Sub-Saharan Africans. Using novel haplotype-based methods here we infer the sub-populations involved in admixture for over 6,500 Latin Americans and evaluate the impact of sub-continental ancestry on the physical appearance of these individuals. We find that pre-Columbian Native genetic structure is mirrored in Latin Americans and that sources of non-Native ancestry, and admixture timings, match documented migratory flows. We also detect South/East Mediterranean ancestry across Latin America, probably stemming from the clandestine colonial migration of Christian converts of non-European origin (Conversos). Furthermore, we find that Central Andean ancestry impacts on variation of facial features in Latin Americans, particularly nose morphology, possibly relating to environmental adaptation during the evolution of Native Americans.

Published
2018

24. Intravitreal bevacizumab in diabetic retinopathy. Recommendations from the Pan-American Collaborative Retina Study Group (PACORES): The 2016 knobloch lecture

Author

Arevalo J.F., Liu T.Y.A., Wu L., Lasave A.F., Farah M., Maia M., Penha F.M., Rodrigues E.B., Morales-Canton V., Fromow-Guerra J., Guerrero-Naranjo J.L., Dalma-Weiszhausz J., Velez-Montoya R., Quiroz-Mercado H., Rodriguez F.J., Gomez F.E., Brieke A.C., Goveto A., Berrocal M.H., Cruz-Villegas V., Graue-Wiechers F., Lozano-Rechy D., Fulda-Graue E., Roca J.A., Hernández A., Saravia M.J., Schlaen A., Rojas J., Ingolotti M., Avila M., Carla L., Cardillo J., Jorge R., Carpentier C., Verdaguer J., Verdaguer J.I., Sepúlveda G., Alezzandrini A., Garcia B., Zas M., Gallego-Pinazo R., Diaz-Llopis M., Dolz-Marco R., Figueroa M., Contreras I., Ruiz-Casas D., and Pan-American Collaborative Retina Study Group (PACORES)

Subjects
0301 basic medicine, medicine.medical_specialty, Bevacizumab, genetic structures, medicine.medical_treatment, Angiogenesis inhibitors, Vitrectomy, Angiogenesis Inhibitors, Angiogenesis inhibitor, Preoperative care, Macular Edema, Tractional retinal detachment, 03 medical and health sciences, adjuvant, Diabetic retinopathy, Diabetic macular edema, Ophthalmology, Preoperative Care, medicine, Adjuvant therapy, Humans, Proliferative diabetic retinopathy, Chemotherapy, Intravitreal bevacizumab, Macular edema, Retina, Intravitreal injections, Diabetic Retinopathy, business.industry, General Medicine, medicine.disease, eye diseases, Adjuvant chemotherapy, 030104 developmental biology, medicine.anatomical_structure, Intravitreal drug administration, Chemotherapy, Adjuvant, Intravitreal Injections, sense organs, business, medicine.drug, Human
Abstract

The advent of intravitreal anti-vascular endothelial growth factor (anti-VEGF) medications has revolutionized the treatment of diabetic eye diseases. Herein, we report the outcomes of clinical studies carried out by the Pan-American Collaborative Retina Study Group (PACORES), with a specific focus on the efficacy of intravitreal bevacizumab in the management of diabetic macular edema and proliferative diabetic retinopathy. We will also discuss the use of intravitreal bevaci-zumab as a preoperative, adjuvant therapy before vitrectomy for prolif-erative diabetic retinopathy. Copyright © 2017 by Asia Pacific Academy of Ophthalmology.

Published
2018

26. Latin Americans show wide-spread Converso ancestry and imprint of local Native ancestry on physical appearance

Author

Chacon-Duque, J-C, Adhikari, K, Fuentes-Guajardo, M, Mendoza-Revilla, J, Acuna-Alonzo, V, Barquera, R, Quinto-Sanchez, M, Gomez-Valdes, J, Everardo Martinez, P, Villamil-Ramirez, H, Hunemeier, T, Ramallo, V, Silva de Cerqueira, CC, Hurtado, M, Villegas, V, Granja, V, Villena, M, Vasquez, R, Llop, E, Sandoval, JR, Salazar-Granara, AA, Parolin, M-L, Sandoval, K, Penaloza-Espinosa, RI, Rangel-Villalobos, H, Winkler, CA, Klitz, W, Bravi, C, Molina, J, Corach, D, Barrantes, R, Gomes, V, Resende, C, Gusmao, L, Amorim, A, Xue, Y, Dugoujon, J-M, Moral, P, Gonzalez-Jose, R, Schuler-Faccini, L, Salzano, FM, Bortolini, M-C, Canizales-Quinteros, S, Poletti, G, Gallo, C, Bedoya, G, Rothhammer, F, Balding, D, Hellenthal, G, Ruiz-Linares, A, Chacon-Duque, J-C, Adhikari, K, Fuentes-Guajardo, M, Mendoza-Revilla, J, Acuna-Alonzo, V, Barquera, R, Quinto-Sanchez, M, Gomez-Valdes, J, Everardo Martinez, P, Villamil-Ramirez, H, Hunemeier, T, Ramallo, V, Silva de Cerqueira, CC, Hurtado, M, Villegas, V, Granja, V, Villena, M, Vasquez, R, Llop, E, Sandoval, JR, Salazar-Granara, AA, Parolin, M-L, Sandoval, K, Penaloza-Espinosa, RI, Rangel-Villalobos, H, Winkler, CA, Klitz, W, Bravi, C, Molina, J, Corach, D, Barrantes, R, Gomes, V, Resende, C, Gusmao, L, Amorim, A, Xue, Y, Dugoujon, J-M, Moral, P, Gonzalez-Jose, R, Schuler-Faccini, L, Salzano, FM, Bortolini, M-C, Canizales-Quinteros, S, Poletti, G, Gallo, C, Bedoya, G, Rothhammer, F, Balding, D, Hellenthal, G, and Ruiz-Linares, A

Abstract

Historical records and genetic analyses indicate that Latin Americans trace their ancestry mainly to the intermixing (admixture) of Native Americans, Europeans and Sub-Saharan Africans. Using novel haplotype-based methods, here we infer sub-continental ancestry in over 6,500 Latin Americans and evaluate the impact of regional ancestry variation on physical appearance. We find that Native American ancestry components in Latin Americans correspond geographically to the present-day genetic structure of Native groups, and that sources of non-Native ancestry, and admixture timings, match documented migratory flows. We also detect South/East Mediterranean ancestry across Latin America, probably stemming mostly from the clandestine colonial migration of Christian converts of non-European origin (Conversos). Furthermore, we find that ancestry related to highland (Central Andean) versus lowland (Mapuche) Natives is associated with variation in facial features, particularly nose morphology, and detect significant differences in allele frequencies between these groups at loci previously associated with nose morphology in this sample.

Published
2018

28. A genome-wide association scan in admixed Latin Americans identifies loci influencing facial and scalp hair features

Author

Adhikari, K, Fontanil, T, Cal, S, Mendoza-Revilla, J, Fuentes-Guajardo, M, Chacon-Duque, J-C, Al-Saadi, F, Johansson, JA, Quinto-Sanchez, M, Acuna-Alonzo, V, Jaramillo, C, Arias, W, Barquera Lozano, R, Macin Perez, G, Gomez-Valdes, J, Villamil-Ramirez, H, Hunemeier, T, Ramallo, V, Silva de Cerqueira, CC, Hurtado, M, Villegas, V, Granja, V, Gallo, C, Poletti, G, Schuler-Faccini, L, Salzano, FM, Bortolini, M-C, Canizales-Quinteros, S, Rothhammer, F, Bedoya, G, Gonzalez-Jose, R, Headon, D, Lopez-Otin, C, Tobin, DJ, Balding, D, Ruiz-Linares, A, Adhikari, K, Fontanil, T, Cal, S, Mendoza-Revilla, J, Fuentes-Guajardo, M, Chacon-Duque, J-C, Al-Saadi, F, Johansson, JA, Quinto-Sanchez, M, Acuna-Alonzo, V, Jaramillo, C, Arias, W, Barquera Lozano, R, Macin Perez, G, Gomez-Valdes, J, Villamil-Ramirez, H, Hunemeier, T, Ramallo, V, Silva de Cerqueira, CC, Hurtado, M, Villegas, V, Granja, V, Gallo, C, Poletti, G, Schuler-Faccini, L, Salzano, FM, Bortolini, M-C, Canizales-Quinteros, S, Rothhammer, F, Bedoya, G, Gonzalez-Jose, R, Headon, D, Lopez-Otin, C, Tobin, DJ, Balding, D, and Ruiz-Linares, A

Abstract

We report a genome-wide association scan in over 6,000 Latin Americans for features of scalp hair (shape, colour, greying, balding) and facial hair (beard thickness, monobrow, eyebrow thickness). We found 18 signals of association reaching genome-wide significance (P values 5 × 10(-8) to 3 × 10(-119)), including 10 novel associations. These include novel loci for scalp hair shape and balding, and the first reported loci for hair greying, monobrow, eyebrow and beard thickness. A newly identified locus influencing hair shape includes a Q30R substitution in the Protease Serine S1 family member 53 (PRSS53). We demonstrate that this enzyme is highly expressed in the hair follicle, especially the inner root sheath, and that the Q30R substitution affects enzyme processing and secretion. The genome regions associated with hair features are enriched for signals of selection, consistent with proposals regarding the evolution of human hair.

Published
2016

29. A genome-wide association scan implicates DCHS2, RUNX2, GLI3, PAX1 and EDAR in human facial variation

Author

Adhikari, K, Fuentes-Guajardo, M, Quinto-Sanchez, M, Mendoza-Revilla, J, Chacon-Duque, JC, Acuna-Alonzo, V, Jaramillo, C, Arias, W, Barquera Lozano, R, Macin Perez, G, Gomez-Valdes, J, Villamil-Ramirez, H, Hunemeier, T, Ramallo, V, Silva de Cerqueira, CC, Hurtado, M, Villegas, V, Granja, V, Gallo, C, Poletti, G, Schuler-Faccini, L, Salzano, FM, Bortolini, M-C, Canizales-Quinteros, S, Cheeseman, M, Rosique, J, Bedoya, G, Rothhammer, F, Headon, D, Gonzalez-Jose, R, Balding, D, Ruiz-Linares, A, Adhikari, K, Fuentes-Guajardo, M, Quinto-Sanchez, M, Mendoza-Revilla, J, Chacon-Duque, JC, Acuna-Alonzo, V, Jaramillo, C, Arias, W, Barquera Lozano, R, Macin Perez, G, Gomez-Valdes, J, Villamil-Ramirez, H, Hunemeier, T, Ramallo, V, Silva de Cerqueira, CC, Hurtado, M, Villegas, V, Granja, V, Gallo, C, Poletti, G, Schuler-Faccini, L, Salzano, FM, Bortolini, M-C, Canizales-Quinteros, S, Cheeseman, M, Rosique, J, Bedoya, G, Rothhammer, F, Headon, D, Gonzalez-Jose, R, Balding, D, and Ruiz-Linares, A

Abstract

We report a genome-wide association scan for facial features in ∼6,000 Latin Americans. We evaluated 14 traits on an ordinal scale and found significant association (P values<5 × 10(-8)) at single-nucleotide polymorphisms (SNPs) in four genomic regions for three nose-related traits: columella inclination (4q31), nose bridge breadth (6p21) and nose wing breadth (7p13 and 20p11). In a subsample of ∼3,000 individuals we obtained quantitative traits related to 9 of the ordinal phenotypes and, also, a measure of nasion position. Quantitative analyses confirmed the ordinal-based associations, identified SNPs in 2q12 associated to chin protrusion, and replicated the reported association of nasion position with SNPs in PAX3. Strongest association in 2q12, 4q31, 6p21 and 7p13 was observed for SNPs in the EDAR, DCHS2, RUNX2 and GLI3 genes, respectively. Associated SNPs in 20p11 extend to PAX1. Consistent with the effect of EDAR on chin protrusion, we documented alterations of mandible length in mice with modified Edar funtion.

Published
2016

31. A genome-wide association study identifies multiple loci for variation in human ear morphology

Author

Adhikari, K, Reales, G, Smith, AJP, Konka, E, Palmen, J, Quinto-Sanchez, M, Acuna-Alonzo, V, Jaramillo, C, Arias, W, Fuentes, M, Pizarro, M, Barquera Lozano, R, Macin Perez, G, Gomez-Valdes, J, Villamil-Ramirez, H, Hunemeier, T, Ramallo, V, Silva de Cerqueira, CC, Hurtado, M, Villegas, V, Granja, V, Gallo, C, Poletti, G, Schuler-Faccini, L, Salzano, FM, Bortolini, M-C, Canizales-Quinteros, S, Rothhammer, F, Bedoya, G, Calderon, R, Rosique, J, Cheeseman, M, Bhutta, MF, Humphries, SE, Gonzalez-Jose, R, Headon, D, Balding, D, Ruiz-Linares, A, Adhikari, K, Reales, G, Smith, AJP, Konka, E, Palmen, J, Quinto-Sanchez, M, Acuna-Alonzo, V, Jaramillo, C, Arias, W, Fuentes, M, Pizarro, M, Barquera Lozano, R, Macin Perez, G, Gomez-Valdes, J, Villamil-Ramirez, H, Hunemeier, T, Ramallo, V, Silva de Cerqueira, CC, Hurtado, M, Villegas, V, Granja, V, Gallo, C, Poletti, G, Schuler-Faccini, L, Salzano, FM, Bortolini, M-C, Canizales-Quinteros, S, Rothhammer, F, Bedoya, G, Calderon, R, Rosique, J, Cheeseman, M, Bhutta, MF, Humphries, SE, Gonzalez-Jose, R, Headon, D, Balding, D, and Ruiz-Linares, A

Abstract

Here we report a genome-wide association study for non-pathological pinna morphology in over 5,000 Latin Americans. We find genome-wide significant association at seven genomic regions affecting: lobe size and attachment, folding of antihelix, helix rolling, ear protrusion and antitragus size (linear regression P values 2 × 10(-8) to 3 × 10(-14)). Four traits are associated with a functional variant in the Ectodysplasin A receptor (EDAR) gene, a key regulator of embryonic skin appendage development. We confirm expression of Edar in the developing mouse ear and that Edar-deficient mice have an abnormally shaped pinna. Two traits are associated with SNPs in a region overlapping the T-Box Protein 15 (TBX15) gene, a major determinant of mouse skeletal development. Strongest association in this region is observed for SNP rs17023457 located in an evolutionarily conserved binding site for the transcription factor Cartilage paired-class homeoprotein 1 (CART1), and we confirm that rs17023457 alters in vitro binding of CART1.

Published
2015

33. Intravitreal bevacizumab (Avastin®) for diabetic retinopathy at 24-months: The 2008 Juan Verdaguer-planas lecture

Author

Arevalo J.F., Sanchez J.G., Lasave A.F., Wu L., Maia M., Bonafonte S., Brito M., Alezzandrini A.A., Restrepo N., Berrocal M.H., Saravia M., Farah M.E., Fromow-Guerra J., Morales-Canton V., Espinoza J.V., Aggio F.B., Quiroz-Mercado H., Guerrero-Naranjo J.L., Rodriguez F.J., Infante R., Medina D., Cruz-Villegas V., Graue-Wiechers F., Lozano-Rechy D., Robledo V., Rodriguez-Loaiza J.L., Roca J.A., Reategui G., Saravia M.J., Martinez-Cartier M., Avila M., Cardillo J., Costa R.A., Verdaguer J., Carpentier C., Verdaguer J.I., Filsecker D.L., Sepúlveda G., Sanchez F., Marini C., Garcia B., and Pan-American Collaborative Retina Study Group (PACORES)

Subjects
Male, optical coherence, endocrine system diseases, genetic structures, Tractional retinal detachment, Diabetic macular edema, Diabetic retinopathy, Proliferative diabetic retinopathy, Treatment outcome, Evaluation, Middle aged, Tomography, Cell proliferation, Priority journal, Intravitreal injections, Bevacizumab, Retrospective study, Macular thickness, Panretinal photocoagulation, Female, Retina macula edema, Human, Monoclonal antibody, Adult, Retina detachment, Visual acuity, Angiogenesis inhibitors, monoclonal, Angiogenesis inhibitor, Follow-up studies, Article, Antibodies, Time, Animals, Humans, Disease severity, Aged, Macular edema, Optical coherence tomography, Animal, Time factors, Diffuse diabetic macular edema, Follow up, Laser coagulation, eye diseases, Drug effect, Retrospective studies, Intravitreal drug administration, sense organs, Vascular endothelial growth factor, Intravitreal bevacizumab
Abstract

Diabetic retinopathy (DR) remains the major threat to sight in the working age population. Diabetic macular edema (DME) is a manifestation of DR that produces loss of central vision. Proliferative diabetic retinopathy (PDR) is a major cause of visual loss in diabetic patients. In PDR, the growth of new vessels is thought to occur as a result of vascular endothelial growth factor (VEGF) release into the vitreous cavity as a response to ischemia. Furthermore, VEGF increases vessel permeability leading to deposition of proteins in the interstitium that facilitate the process of angiogenesis and macular edema. This review demonstrates multiple benefits of intravitreal bevacizumab (IVB) on DR including DME and PDR at 24 months of follow up. The results indicate that IVB injections may have a beneficial effect on macular thickness and visual acuity (VA) in diffuse diabetic macular edema. Therefore, in the future this new therapy could replace or complement focal/grid laser photocoagulation in DME. In PDR, this new option could be an adjuvant agent to pan-retina photocoagulation so that more selective therapy may be applied. In addition, we report a series of patients in which tractional retinal detachment developed or progressed after adjuvant preoperative IVB in severe PDR. © 2010 Bentham Science Publishers Ltd.

Published
2010

34. Aplication of authenticity criteria in mitochondrial studies on archaic bone remains from a prehispanic Muisca population

Author

Jara, N. P., Díaz, M., Villegas, V., Mesa, C. L., Torres, D., Bernal, J., Alberto Gómez, and Briceño, I.

Subjects
Archeology, Base pairing, Ethnography, Muisca, DNA determination, Bone age, Archaic DNA, Colombia, Population biology, Article, Mitochondrial DNA, Restriction mapping, Sample, Human experiment, Chibcha, American Indian, Haplotype, Genetic variability, Geographic distribution, Antiquity, Controlled study, Human
Abstract

Texto plano de página web Introducción: Los estudios de ADN arcaico (aADN) pueden verificar hipótesis sobre las poblaciones del pasado, permiten analizar la composición genética, pudiéndose así soslayar sesgos introducidos por migraciones, expansiones demográficas, mutaciones y cuellos de botella acontecidos con posterioridad. Los métodos de análisis son objeto de cuidadoso procedimiento debido a la dificultad de recuperación y a la posibilidad de contaminación, por lo tanto es necesario establecer protocolos que garanticen la reproducibilidad y veracidad de los resultados. Objetivo: El presente estudio tiene como fin establecer un protocolo para la obtención de ADN arcaico en 16 muestras óseas precolombinas encontradas en una excavación del sector de Candelaria La Nueva, Bogotá, Colombia, fechadas dentro del período Muisca Tardío. Métodos: Se estudiaron 4 haplogrupos fundadores amerindios presentes en el ADN mitocondrial arcaico, mediante enzimas de restricción con base en estudios de alta resolución obteniendo fragmentos de tamaños entre 121 y 186 pares de bases (pb). Los distintos análisis se realizaron observando un estricto control de los criterios de autenticidad en relación con las condiciones de laboratorio, incluyendo el uso de controles y blancos, la reproducibilidad de resultados y la verificación de las características particulares que presenta el ADN arcaico. Resultados: Los resultados obtenidos de las muestras óseas arcaicas establecieron la presencia única del haplogrupo «A» en la población estudiada. Estos datos apoyan la afirmación de los arqueólogos en cuanto a que se trata de una misma población biológica tanto espacial como temporalmente. La distribución de este haplogrupo en una frecuencia del 100% soporta la filiación genética con los grupos chibcha. Conclusión: Este trabajo es una contribución al estudio de la diversidad genética en las poblaciones americanas antiguas, permitiendo abordar la integración de datos geográficos e históricos con técnicas de caracterización genética asociados con patrones lingüísticos, etnogeográficos y glotocronológicos.

Published
2010

36. Psychrophilic anaerobic digestion of guinea pig manure in low-cost tubular digesters at high altitude

Author

Universitat Politècnica de Catalunya. Departament d'Enginyeria Hidràulica, Marítima i Ambiental, Universitat Politècnica de Catalunya. Departament d'Enginyeria Mecànica, Universitat Politècnica de Catalunya. GEMMA - Grup d'Enginyeria i Microbiologia del Medi Ambient, Universitat Politècnica de Catalunya. EOLI - Enginyeria d'Organització i Logística Industrial, Garfi, Marianna, Ferrer Martí, Laia, Villegas, V., Ferrer Martí, Ivet, Universitat Politècnica de Catalunya. Departament d'Enginyeria Hidràulica, Marítima i Ambiental, Universitat Politècnica de Catalunya. Departament d'Enginyeria Mecànica, Universitat Politècnica de Catalunya. GEMMA - Grup d'Enginyeria i Microbiologia del Medi Ambient, Universitat Politècnica de Catalunya. EOLI - Enginyeria d'Organització i Logística Industrial, Garfi, Marianna, Ferrer Martí, Laia, Villegas, V., and Ferrer Martí, Ivet

Abstract

Guinea pig is one of the most common livestock in rural communities of the Andes. The aim of this research was to study the anaerobic digestion of guinea pig manure in low-cost unheated tubular digesters at high altitude. To this end, the performance of two pilot digesters was monitored during 7 months; and two greenhouse designs were compared. In the dome roof digester the temperature and biogas production were significantly higher than in the shed roof digester. However, the biogas production rate was low (0.04 View the MathML sourcembiogas3View the MathML sourcemdigester-3 d−1), which is attributed to the low organic loading rate (0.6 kgVS View the MathML sourcemdigester-3 d−1) and temperature (23 °C) of the system, among other factors. In a preliminary fertilization study, the potato yield per hectare was increased by 100% using the effluent as biofertilizer. Improving manure management techniques, increasing the organic loading rate and co digesting other substrates may be considered to enhance the process., Peer Reviewed, Postprint (published version)

Published
2011

41. Renal histopathology

Author

Marie-Lucile, F., primary, Laure-Helene, N., additional, Yosr, C., additional, Anne, M., additional, Fadi, F., additional, Levi, C., additional, Meas-Yedid, V., additional, Daniliuc, C., additional, Karras, A., additional, Olivo-Marin, J. C., additional, Mouthon, L., additional, Guiard, E., additional, Roland, M., additional, Guillevin, L., additional, Jacquot, C., additional, Nochy, D., additional, Thervet, E., additional, Chen, Q., additional, Skerka, C., additional, Uzonyi, B., additional, Lindner, S., additional, Licht, C., additional, Hoppe, B., additional, Riedl, M., additional, Kirschfink, M., additional, Habbich, S., additional, Wolf, G., additional, Strain, L., additional, Goodship, T. H., additional, Zipfel, P. F., additional, Kfoury, H., additional, Alsuwaida, A., additional, Alsaad, K., additional, Alhejaili, F., additional, Alghonaim, M., additional, Alwakeel, J., additional, Husain, S., additional, Aloudah, N., additional, Besso, L., additional, Tamagnone, M., additional, Daidola, G., additional, Burdese, M., additional, Repetto, L., additional, Pasquale, G., additional, Colla, L., additional, Biancone, L., additional, Stratta, P., additional, Segoloni, G. P., additional, Bacalja, J., additional, Bauer Segvic, A. M., additional, Bulimbasic, S., additional, Pacic, A., additional, Knotek, M., additional, Sabljar Matovinovic, M., additional, Galesic, K., additional, Galesic Ljubanovic, D., additional, Zakharova, E., additional, Stolyarevich, E., additional, Vorobjova, O., additional, Tamouza, H., additional, Chemouny, J. M., additional, Flamant, M., additional, Raskova Kafkova, L., additional, Demion, M., additional, Laurent, M., additional, Walker, F., additional, Julian, B. A., additional, Tissandie, E., additional, Tiwari, M. K., additional, Novak, J., additional, Camara, N. O., additional, Benhamou, M., additional, Vrtovsnik, F., additional, Monteiro, R. C., additional, Moura, I. C., additional, Samavat, S., additional, Ahmadpoor, P., additional, Torbati, P., additional, Ghaderi, R., additional, Poorrezagholi, F., additional, Samadian, F., additional, Nafar, M., additional, MII, A., additional, Shimizu, A., additional, Kaneko, T., additional, Yasuda, F., additional, Fukui, M., additional, Masuda, Y., additional, Iino, Y., additional, Katayama, Y., additional, Muller, C., additional, Markovic-Lipkovski, J., additional, Simic-Ogrizovic, S., additional, Naumovic, R., additional, Cirovic, S., additional, Mitrovic, D., additional, Muller, G., additional, Wozniak, A., additional, Janicka-Jedynska, M., additional, Zurawski, J., additional, Kaczmarek, E., additional, Zachwieja, J., additional, Khilji, S., additional, Dorman, T., additional, O'kelly, P., additional, Lampty, L., additional, Leung, K., additional, Shadivan, A., additional, Varghese, C., additional, Walshe, J., additional, Saito, T., additional, Kawano, M., additional, Saeki, T., additional, Mizushima, I., additional, Yamaguchi, Y., additional, Imai, N., additional, Nakashima, H., additional, Umehara, H., additional, Shvetsov, M., additional, Popova, O., additional, Chebotareva, N., additional, Ivanov, A., additional, Bobkova, I., additional, Cremasco, D., additional, Ceol, M., additional, Peruzzi, L., additional, Mazzucco, G., additional, Giuseppina, M., additional, Vezzoli, G., additional, Cristofaro, R., additional, D'angelo, A., additional, Anglani, F., additional, Del Prete, D., additional, Coppolino, G., additional, Comi, N., additional, Bolignano, D., additional, Piraina, V., additional, Talarico, R., additional, Colombo, A., additional, Lucisano, G., additional, Fuiano, G., additional, Bernich, P., additional, Lupo, A., additional, Of Renal Biopsies, T. R., additional, Rastaldi, M. P., additional, Jercan, O. C., additional, Messa, P., additional, Alexandru, D., additional, Mogoanta, L., additional, and Uribe Villegas, V., additional

Published
2012
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46. Aplication of authenticity criteria in mitochondrial studies on archaic bone remains from a prehispanic muisca population.

Author

JARA NP, DÍAZ M, VILLEGAS V, DE MESA CL, TORRES D, BERNAL J, G?MEZ A, and BRICEÑO I

Abstract

Introduction: Ancient DNA (aDNA)studies can support hypotheses regarding ancient populations; molecular studies can analyze the local population's genetic composition, minimizing biases introduced by later migrations, demographic expansions, mutations, and bottleneck effects. These analyses must be performed with special care because of the low DNA concentrations and contamination risk; therefore, it is necessary to establish protocols to guarantee the reproducibility and veracity of results. Objective: The present study aims to establish a protocol to obtain ancient DNA from 16 pre-Columbian bone samples found in an excavation performed in the area «Candelaria La Nueva» in Bogota, Colombia, dated in the period «Muisca Tardio». Methods: Four founder mitochondrial DNA Amerindian haplotypes were analyzed by high resolution restriction enzyme analyses, obtaining fragments between 121 and 186 base pairs (bp). Different analyses were performed following a strict control of authenticity criteria regarding laboratory conditions, including: positive and negative controls, reproducibility of results, and verification of particular characteristics present in ancient DNA. Results: Results obtained from the bone samples showed the exclusive presence of haplogroup A in the population studied. This data support the statement of the archaeologists of a single biological population in space and time. The distribution of this haplogroup in a 100% frequency supports the hypothesis of Chibcha genetic affiliation. Conclusion: The present study is a contribution to the study of genetic diversity in archaic American populations, allowing the integration of geographic and historic data with genetic characterization techniques associated with linguistic, ethnographic, and glottochronology patterns. Following the protocol proposed in the present study allows fulfilling authenticity criteria for ancient samples with the available techniques. [ABSTRACT FROM AUTHOR]

Published
2010

47. Preliminary population study of Methylenetetrahydrofolate Reductase (MTHFR) C677T polymorphism determination in a pilot group of students from the University of Rosario.

Author

González-Galofre ZN, Villegas V, and Martínez-Agüero M

Abstract

Copyright of Revista Ciencias de la Salud is the property of Colegio Mayor de Nuestra Senora del Rosario and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2010

48. Anatomic and Visual Outcomes of 23-G Vitrectomy without Scleral Buckling for Primary Rhegmatogenous Retinal Detachment

Author

Figueroa, Marta S., Contreras, Inés, Noval, Susana, Wu, L., Arevalo, J. F., Serrano, M. A., Lasave, A. F., Farah, M., Maia, M., Penha, F. M., Rodrigues, E. B., Morales-Canton, V., Fromow-Guerra, J., Guerrero-Naranjo, J.L., Dalma-Weiszhausz, J., Quiroz-Mercado, H., Velez-Montoya, R., Rodriguez, F. J., Gomez, F. E., Brieke, A. C., Berrocal, M.H., Cruz-Villegas, V., Graue-Wiechers, F., Lozano-Rechy, D., Ariza-Camacho, E., Roca, J. A., Chico, R. G., Saravia, M. J., Schlaen, A., Lupinacci, A., Gabin, M. N., Avila, M., Carla, L., Cardillo, J., Verdaguer, J., Carpentier, T. C., Verdaguer, J. I., Sepúlveda, D. G., Alezzandrini, A., Garcia, B., Bregliano, G., Alvira, G., Flor, P., Jaramillo, F., Diaz-Llopis, M., Gallego-Pinazo, R., Udaondo, P., Salom, D., Figueroa, M., Contreras, I., and Ruiz-Casas, D.

Abstract

Purpose To evaluate the anatomic success rate, visual acuity (VA) changes, and complications of 23-G vitrectomy without associated scleral procedures for the treatment of primary rhegmatogenous retinal detachment (PRRD).Methods Patients diagnosed with PRRD were considered for inclusion. Patients with evidence of proliferative vitreoretinopathy or coexisting ocular pathologies were excluded. Surgery consisted of 23-G vitrectomy with endolaser photocoagulation of retinal breaks and fluid-air-gas (12% C3F8) exchange. Minimum follow-up was 3 months.Results A total of 133 eyes of 118 patients were included. Fifty eyes were phakic and 83 pseudophakic. Mean time from diagnosis to surgery was 6.9 days (range 1–40). Mean VA improved significantly from 20/50 (range: hand movements to 20/20) to 20/30 (range: counting fingers to 20/16), with no statistically significant differences between phakic and pseudophakic eyes (p = 0.233). Visual acuity improved to 20/40 or better in 104 eyes (78.2%). A redetachment developed in 5 eyes (3.8%), so the primary anatomic success rate was 96.2%. Four eyes required a second surgical procedure and one eye a third to achieve retinal reattachment. Cataract progression in phakic eyes made cataract surgery necessary within 1 year of vitrectomy in 12/50 (24%) eyes. Subretinal perfluoro-N-octane (PFO) was detected in 6 eyes (4.5%).Conclusion Twenty-three–gauge vitrectomy without scleral buckling seems to be an effective technique for the treatment of PRRD without proliferative vitreoretinopathy. The high anatomic success rate was comparable to that previously described with 20 G and scleral buckling. Complications were similar to those described for 20-G vitrectomy, except for retained subretinal PFO, which has a higher rate.

Published
2013
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50. Mapping the pro-region of carboxypeptidase B by protein engineering. Cloning, overexpression, and mutagenesis of the porcine proenzyme.

Author

Ventura, S, Villegas, V, Sterner, J, Larson, J, Vendrell, J, Hershberger, C L, and Avilés, F X

Abstract

The proteolytic processing of pancreatic procarboxypeptidase B to a mature and functional enzyme is much faster than that of procarboxypeptidase A1. This different behavior has been proposed to depend on specific conformational features at the region that connects the globular domain of the pro-segment to the enzyme and at the contacting surfaces on both moieties. A cDNA coding for porcine procarboxypeptidase B was cloned, sequenced, and expressed at high yield (250 mg/liter) in the methylotrophic yeast Pichia pastoris. To test the previous hypothesis, different mutants of the pro-segment at the putative tryptic targets in its connecting region and at some of the residues contacting the active enzyme were obtained. Moreover, the complete connecting region was replaced by the homologous sequence in procarboxypeptidase A1. The detailed study of the tryptic processing of the mutants shows that limited proteolysis of procarboxypeptidase B is a very specific process, as Arg-95 is the only residue accessible to tryptic attack in the proenzyme. A fast destabilization of the connecting region after the first tryptic cut allows subsequent proteolytic processing and the expression of carboxypeptidase B activity. Although all pancreatic procarboxypeptidases have a preformed active site, only the A forms show intrinsic activity. Mutational substitution of Asp-41 in the globular activation domain, located at the interface with the enzyme moiety, as well as removal of the adjacent 310 helix allow the appearance of residual activity in the mutated procarboxypeptidase B, indicating that the interaction of both structural elements with the enzyme moiety prevents the binding of substrates and promotes enzyme inhibition. In addition, the poor heterologous expression of such mutants indicates that the mutated region is important for the folding of the whole proenzyme.

Published
1999

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