13 results on '"Villasanta-Gonzalez A"'
Search Results
2. Plasma lipidic fingerprint associated with type 2 diabetes in patients with coronary heart disease: CORDIOPREV study
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Alejandro Villasanta-Gonzalez, Marina Mora-Ortiz, Juan F. Alcala-Diaz, Lorenzo Rivas-Garcia, Jose D. Torres-Peña, Asuncion Lopez-Bascon, Monica Calderon-Santiago, Antonio P. Arenas-Larriva, Feliciano Priego‑Capote, Maria M. Malagon, Fabian Eichelmann, Pablo Perez-Martinez, Javier Delgado-Lista, Matthias B. Schulze, Antonio Camargo, and Jose Lopez-Miranda
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LC–MS ,Random survival forest ,Lipidomic risk score ,Cox ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Objective We aimed to identify a lipidic profile associated with type 2 diabetes mellitus (T2DM) development in coronary heart disease (CHD) patients, to provide a new, highly sensitive model which could be used in clinical practice to identify patients at T2DM risk. Methods This study considered the 462 patients of the CORDIOPREV study (CHD patients) who were not diabetic at the beginning of the intervention. In total, 107 of them developed T2DM after a median follow-up of 60 months. They were diagnosed using the American Diabetes Association criteria. A novel lipidomic methodology employing liquid chromatography (LC) separation followed by HESI, and detection by mass spectrometry (MS) was used to annotate the lipids at the isomer level. The patients were then classified into a Training and a Validation Set (60–40). Next, a Random Survival Forest (RSF) was carried out to detect the lipidic isomers with the lowest prediction error, these lipids were then used to build a Lipidomic Risk (LR) score which was evaluated through a Cox. Finally, a production model combining the clinical variables of interest, and the lipidic species was carried out. Results LC-tandem MS annotated 440 lipid species. From those, the RSF identified 15 lipid species with the lowest prediction error. These lipids were combined in an LR score which showed association with the development of T2DM. The LR hazard ratio per unit standard deviation was 2.87 and 1.43, in the Training and Validation Set respectively. Likewise, patients with higher LR Score values had lower insulin sensitivity (P = 0.006) and higher liver insulin resistance (P = 0.005). The receiver operating characteristic (ROC) curve obtained by combining clinical variables and the selected lipidic isomers using a generalised lineal model had an area under the curve (AUC) of 81.3%. Conclusion Our study showed the potential of comprehensive lipidomic analysis in identifying patients at risk of developing T2DM. In addition, the lipid species combined with clinical variables provided a new, highly sensitive model which can be used in clinical practice to identify patients at T2DM risk. Moreover, these results also indicate that we need to look closely at isomers to understand the role of this specific compound in T2DM development. Trials registration NCT00924937.
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- 2023
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3. A plasma fatty acid profile associated to type 2 diabetes development: from the CORDIOPREV study
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Villasanta-Gonzalez, Alejandro, Alcala-Diaz, Juan Francisco, Vals-Delgado, Cristina, Arenas, Antonio Pablo, Cardelo, Magdalena P., Romero-Cabrera, Juan Luis, Rodriguez-Cantalejo, Fernando, Delgado-Lista, Javier, Malagon, Maria M., Perez-Martinez, Pablo, Schulze, Matthias B., Camargo, Antonio, and Lopez-Miranda, Jose
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- 2022
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4. Beta cell functionality and hepatic insulin resistance are major contributors to type 2 diabetes remission and starting pharmacological therapy: from CORDIOPREV randomized controlled trial
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Roncero-Ramos, Irene, Gutierrez-Mariscal, Francisco M., Gomez-Delgado, Francisco, Villasanta-Gonzalez, Alejandro, Torres-Peña, Jose D., Cruz-Ares, Silvia De La, Rangel-Zuñiga, Oriol A., Luque, Raul M., Ordovas, Jose M., Delgado-Lista, Javier, Perez-Martinez, Pablo, Camargo, Antonio, Alcalá-Diaz, Juan F., and Lopez-Miranda, Jose
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- 2021
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5. Diabetes Remission Is Modulated by Branched Chain Amino Acids According to the Diet Consumed: From the CORDIOPREV Study
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Cardelo, Magdalena P., primary, Alcala‐Diaz, Juan F., additional, Gutierrez‐Mariscal, Francisco M., additional, Lopez‐Moreno, Javier, additional, Villasanta‐Gonzalez, Alejandro, additional, Arenas‐de Larriva, Antonio P., additional, Cruz‐Ares, Silvia de la, additional, Delgado‐Lista, Javier, additional, Rodriguez‐Cantalejo, Fernando, additional, Luque, Raul M., additional, Ordovas, Jose M., additional, Perez‐Martinez, Pablo, additional, Camargo, Antonio, additional, and Lopez‐Miranda, Jose, additional
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- 2022
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6. A plasma fatty acid profile associated to type 2 diabetes development: from the CORDIOPREV study
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Villasanta-Gonzalez, Alejandro, primary, Alcala-Diaz, Juan Francisco, additional, Vals-Delgado, Cristina, additional, Arenas, Antonio Pablo, additional, Cardelo, Magdalena P., additional, Romero-Cabrera, Juan Luis, additional, Rodriguez-Cantalejo, Fernando, additional, Delgado-Lista, Javier, additional, Malagon, Maria M., additional, Perez-Martinez, Pablo, additional, Schulze, Matthias B., additional, Camargo, Antonio, additional, and Lopez-Miranda, Jose, additional
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- 2021
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7. Beta cell functionality and hepatic insulin resistance are major contributors to type 2 diabetes remission and starting pharmacological therapy: from CORDIOPREV randomized controlled trial
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Oriol A. Rangel-Zuñiga, Antonio Camargo, Francisco M. Gutierrez-Mariscal, Jose D Torres-Peña, Silvia de la Cruz-Ares, Alejandro Villasanta-Gonzalez, Jose Lopez-Miranda, Raúl M. Luque, Javier Delgado-Lista, Irene Roncero-Ramos, Jose M. Ordovas, Pablo Perez-Martinez, Francisco Gomez-Delgado, Juan F. Alcala-Diaz, Univ Cordoba, Maimonides Biomed Res Inst Cordoba IMIBIC, Dept Internal Med, Lipids & Atherosclerosis Unit,Reina Sofia Univ Ho, Cordoba, SpainInst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr CIBEROBN, Cordoba, SpainMaimonides Inst Biomed Res Cordoba IMIBIC, Cordoba, SpainUniv Cordoba, Dept Cell Biol Physiol & Immunol, Cordoba, SpainTufts Univ, JM US Dept Agr Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USACNIC, IMDEA Alimentac, Madrid, Spain, Fundacion Patrimonio Comunal Olivarero, Junta de Andalucia (Consejeria de Salud, Consejeria de Agricultura y Pesca, Consejeria de Innovacion, Ciencia y Empresa), Diputaciones de Jaen y Cordoba, Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud, Ministerio de Medio Ambiente, Medio Rural y Marino, Gobierno de Espana, Ministerio de Economia y Competitividad, Instituto de Salud Carlos III (ISCIII) of Spain, Directorate General for Assessment and Promotion of Research, EU's European Regional Development Fund (FEDER), Universidad de Cordoba/CBUA, ISCIII postdoctoral research contract, and CIBEROBN postdoctoral research contract
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Male ,Fat accumulation ,Disease ,Type 2 diabetes ,Glucose-production ,Diet, Mediterranean ,Gastroenterology ,law.invention ,Glycemic control ,Sensitivity ,Randomized controlled trial ,law ,Weight loss ,Insulin-Secreting Cells ,Fatty Acids ,Alanine Transaminase ,General Medicine ,Middle Aged ,Liver ,Female ,medicine.symptom ,Beta cell ,medicine.medical_specialty ,Hepatic insulin resistance ,Pathophysiology ,Insulin resistance ,Cardiovascular-disease ,Physiology (medical) ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,Hypoglycemic Agents ,Secretion ,Glycated Hemoglobin ,business.industry ,Biochemistry (medical) ,Public Health, Environmental and Occupational Health ,Glucose Tolerance Test ,CORDIOPREV ,Intervention studies ,medicine.disease ,Clinical trial ,Type 2 Diabetes Mellitus ,Diabetes Mellitus, Type 2 ,Dysfunction ,Weight-loss ,Insulin Resistance ,business ,Tolerance - Abstract
In order to assess whether previous hepatic IR (Hepatic-IRfasting) and beta-cell functionality could modulate type 2 diabetes remission and the need for starting glucose-lowering treatment, newly-diagnosed type 2 diabetes participants who had never received glucose-lowering treatment (190 out of 1002) from the CORDIOPREV study (a prospective, randomized and controlled clinical trial), were randomized to consume a Mediterranean or a low-fat diet. Type 2 diabetes remission was defined according to the ADA recommendation for levels of HbA1c, fasting plasma glucose and 2h plasma glucose after OGTT, and having maintained them for at least 2 consecutive years. Patients were classified according to the median of Hepatic-IRfasting and beta-cell functionality, measured as the disposition index (DI) at baseline. Cox proportional hazards regression determined the potential for Hepatic-IRfasting and DI indexes as predictors of diabetes remission and the probability of starting pharmacological treatment after a five-year follow-up. Low-Hepatic-IRfasting or high-DI patients had a higher probability of diabetes remission than high-Hepatic-IRfasting or low-DI subjects (HR:1.79;95%CI 1.06-3.05; and HR:2.66;95%CI 1.60-4.43, respectively) after a dietary intervention with no pharmacological treatment and no weight loss. The combination of low-Hepatic-IRfasting and high-DI presented the highest probability of remission (HR:4.63;95%CI 2.00-10.70). Among patients maintaining diabetes, those with high- Hepatic-IRfasting and low-DI showed the highest risk of starting glucose-lowering therapy (HR:3.24;95%CI 1.50-7.02). Newly-diagnosed type 2 diabetes patients with better beta-cell functionality and lower Hepatic-IRfasting had a higher probability of type 2 diabetes remission in a dietary intervention without pharmacological treatment or weight loss, whereas among patients not achieving remission, those with worse beta-cell functionality and higher Hepatic-IRfasting index had the highest risk of starting glucose-lowering treatment after five years of follow-up. BACKGROUND: Our current knowledge regarding the etiology of type 2 diabetes points to hepatic insulin resistance and beta cell functionality as two major abnormalities underlying the disease. Previous studies have associated type 2 diabetes remission with weight loss, together with a decrease in liver fat content and a higher beta cell recovery. TRANSLATIONAL SIGNIFICANCE: Patients with lower hepatic insulin resistance and better beta cell functionality had a higher probability of remission without significant weight loss or pharmacological treatment. These results suggest that clinicians could identify patients with specific phenotypes in early-diagnosed type 2 diabetes that could be the key to achieve higher remission rates without weight loss or pharmacological treatment.
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- 2021
8. A plasma fatty acid profile associated to type 2 diabetes development: from the CORDIOPREV study
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Javier Delgado-Lista, Alejandro Villasanta-Gonzalez, Cristina Vals-Delgado, Magdalena P. Cardelo, Jose Lopez-Miranda, Pablo Perez-Martinez, Antonio Camargo, Fernando Rodriguez-Cantalejo, Matthias B. Schulze, María M. Malagón, Juan Luis Romero-Cabrera, Antonio Pablo Arenas, and Juan F. Alcala-Diaz
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medicine.medical_specialty ,endocrine system diseases ,FA Score ,Medicine (miscellaneous) ,Coronary Disease ,Type 2 diabetes ,Gastroenterology ,Insulin resistance ,Internal medicine ,Medicine ,Humans ,In patient ,Disease prediction ,Fatty acids ,American diabetes association ,chemistry.chemical_classification ,Nutrition and Dietetics ,Training set ,business.industry ,Fatty Acids ,COX ,Insulin sensitivity ,Type 2 Diabetes Mellitus ,Fatty acid ,nutritional and metabolic diseases ,medicine.disease ,chemistry ,Diabetes Mellitus, Type 2 ,Insulin Resistance ,business ,Biomarkers - Abstract
Purpose The prevalence of type 2 diabetes mellitus (T2DM) is increasing worldwide. For this reason, it is essential to identify biomarkers for the early detection of T2DM risk and/or for a better prognosis of T2DM. We aimed to identify a plasma fatty acid (FA) profile associated with T2DM development. Methods We included 462 coronary heart disease patients from the CORDIOPREV study without T2DM at baseline. Of these, 107 patients developed T2DM according to the American Diabetes Association (ADA) diagnosis criteria after a median follow-up of 60 months. We performed a random classification of patients in a training set, used to build a FA Score, and a Validation set, in which we tested the FA Score. Results FA selection with the highest prediction power was performed by random survival forest in the Training set, which yielded 4 out of the 24 FA: myristic, petroselinic, α-linolenic and arachidonic acids. We built a FA Score with the selected FA and observed that patients with a higher score presented a greater risk of T2DM development, with an HR of 3.15 (95% CI 2.04–3.37) in the Training set, and an HR of 2.14 (95% CI 1.50–2.84) in the Validation set, per standard deviation (SD) increase. Moreover, patients with a higher FA Score presented lower insulin sensitivity and higher hepatic insulin resistance (p Conclusion Our results suggest that a detrimental FA plasma profile precedes the development of T2DM in patients with coronary heart disease, and that this FA profile can, therefore, be used as a predictive biomarker. Clinical Trials.gov.Identifier NCT00924937.
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- 2021
9. A Diet-Dependent Microbiota Profile Associated with Incident Type 2 Diabetes: From the CORDIOPREV Study
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Javier Delgado-Lista, Pablo Perez-Martinez, Antonio Camargo, Carmen Haro, Alejandro Villasanta-Gonzalez, Cristina Vals-Delgado, Magdalena P. Cardelo, Juan F. Alcala-Diaz, Francisco Gomez-Delgado, Jose Lopez-Miranda, Jose D Torres-Peña, María M. Malagón, Jose M. Ordovas, Ipek Guler, Ana Leon-Acuña, Instituto de Salud Carlos III, Junta de Andalucía, European Commission, and Ministerio de Economía y Competitividad (España)
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0301 basic medicine ,endocrine system diseases ,Intestinal microbiota ,Physiology ,Type 2 diabetes ,Gut flora ,Individual risk ,03 medical and health sciences ,Gammaproteobacteria ,Prevotella ,Medicine ,In patient ,Microbiome ,Feces ,030109 nutrition & dietetics ,biology ,business.industry ,nutritional and metabolic diseases ,CORDIOPREV ,medicine.disease ,biology.organism_classification ,Diet ,030104 developmental biology ,Predictive model ,business ,Food Science ,Biotechnology - Abstract
Scope The differences between the baseline gut microbiota of patients who developed type 2 diabetes (T2D) consuming a low‐fat (LF) or a Mediterranean (Med) diet are explored and risk scores are developed to predict the individual risk of developing T2D associated with the consumption of LF or Med diet. Methods and Results All the patients from the CORDIOPREV study without T2D at baseline (n = 462) whose fecal sample are available, are included. Gut microbiota is analyzed by 16S sequencing and the risk of T2D after a median follow‐up of 60 months assessed by Cox analysis. Linear discriminant analysis effect size (LEfSe) analysis shows a different baseline gut microbiota in patients who developed T2D consuming LF and Med diets. A higher abundance of Paraprevotella, and lower Gammaproteobacteria and B. uniformis are associated with T2D risk when an LF diet is consumed. In contrast, higher abundances of Saccharibacteria, Betaproteobacteria, and Prevotella are associated with T2D risk when a Med diet is consumed. Conclusion The results suggest that different interactions between the microbiome and dietary patterns may partially determine the risk of T2D development, which may be used for selecting personalized dietary models to prevent T2D., Agencia de Innovación y Desarrollo de Andalucía. Grant Number: CVI‐7450 Instituto de Salud Carlos III. Grant Numbers: CP14/00114, CPII19/00007, DTS19/00007, FIS PI13/00023, PI16/01777, PI19/00299, PIE 14/00031, PIE14/00005 European Regional Development Fund. Grant Number: na Secretaría de Estado de Investigación, Desarrollo e Innovación. Grant Number: AGL2015‐67896‐P
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- 2020
10. Postprandial Lipemia Modulates Pancreatic Alpha-Cell Function in the Prediction of Type 2 Diabetes Development: The CORDIOPREV Study
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Alejandro Villasanta-Gonzalez, Irene Roncero-Ramos, Elena M. Yubero-Serrano, Raúl M. Luque, Ivan Quesada, Ana Leon-Acuña, Jose Lopez-Miranda, Isabel Pozuelo-Sanchez, Javier Caballero-Villarraso, Anabel Gonzalez-Requero, Cristina Vals-Delgado, Pablo Perez-Martinez, Jose M. Ordovas, and Juan F. Alcala-Diaz
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0106 biological sciences ,Male ,medicine.medical_specialty ,endocrine system diseases ,Coronary Disease ,Hyperlipidemias ,Type 2 diabetes ,01 natural sciences ,Glucagon ,Alpha cell ,Diabetes mellitus ,Internal medicine ,medicine ,Humans ,Prospective Studies ,Triglycerides ,business.industry ,010401 analytical chemistry ,Hazard ratio ,Area under the curve ,nutritional and metabolic diseases ,Type 2 Diabetes Mellitus ,General Chemistry ,Middle Aged ,medicine.disease ,Lipid Metabolism ,Postprandial Period ,0104 chemical sciences ,Endocrinology ,Postprandial ,Diabetes Mellitus, Type 2 ,Glucagon-Secreting Cells ,Disease Progression ,Female ,General Agricultural and Biological Sciences ,business ,010606 plant biology & botany - Abstract
Diabetes (T2DM) is a major global health issue, and developing new approaches to its prevention is of paramount importance. We hypothesized that abnormalities in lipid metabolism are involved in alpha-cell deregulation. We therefore studied the metabolic factors underlying alpha-cell dysfunction in T2DM progression after a dietary intervention (Mediterranean and low-fat). Additionally, we evaluated whether postprandial glucagon levels may be considered as a predictive factor of T2DM in cardiovascular patients. Non-T2DM participants from the CORDIOPREV study were categorized by tertiles of the area under the curve (AUC) for triacylglycerols and also by tertiles of AUC for glucagon. Our results showed that patients with higher triacylglycerols levels presented elevated postprandial glucagon (P = 0.009). Moreover, we observed higher risk of T2DM (hazard ratio: 2.65; 95% confidence interval: 1.56-4.53) in subjects with elevated glucagon. In conclusion, high postprandial lipemia may induce alpha-cell dysfunction in cardiovascular patients. Our results also showed that postprandial glucagon levels could be used to predict T2DM development.
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- 2020
11. Dietary administration of the probiotic Shewanella putrefaciens Pdp11 promotes transcriptional changes of genes involved in growth and immunity in Solea senegalensis larvae
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Alejandro Villasanta-Gonzalez, M.C. Balebona, Silvana Teresa Tapia-Paniagua, Juan Jurado, Miguel Ángel Moriñigo, Inés García de la Banda, and María-José Prieto-Álamo
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Fish Proteins ,0301 basic medicine ,Transcription, Genetic ,media_common.quotation_subject ,Shewanella putrefaciens ,Aquatic Science ,Biology ,Microbiology ,law.invention ,03 medical and health sciences ,Probiotic ,Immune system ,Immunity ,law ,Animals ,Environmental Chemistry ,Juvenile ,Weaning ,Metamorphosis ,media_common ,Larva ,Probiotics ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,Animal Feed ,Diet ,030104 developmental biology ,Flatfishes ,040102 fisheries ,0401 agriculture, forestry, and fisheries - Abstract
Senegalese sole (Solea senegalensis) has been proposed as a high-potential species for aquaculture diversification in Southern Europe. It has been demonstrated that a proper feeding regimen during the first life stages influences larval growth and survival, as well as fry and juvenile quality. The bacterial strain Shewanella putrefaciens Pdp11 (SpPdp11) has shown very good probiotic properties in Senegalese sole, but information is scarce about its effect in the earliest stages of sole development. Thus, the aim of this study was to investigate the effect of SpPdp11, bioencapsulated in live diet, administered during metamorphosis (10-21 dph) or from the first exogenous feeding of Senegalese sole (2-21 dph). To evaluate the persistence of the probiotic effect, we sampled sole specimens from metamorphosis until the end of weaning (from 23 to 73 dph). This study demonstrated that probiotic administration from the first exogenous feeding produced beneficial effects on Senegalese sole larval development, given that specimens fed this diet exhibited higher and less dispersed weight, as well as increases in both total protein concentration and alkaline phosphatase activity, and in non-specific immune response. Moreover, real-time PCR documented changes in the expression of a set of genes involved in central metabolic functions including genes related to growth, genes coding for proteases (including several digestive enzymes), and genes implicated in the response to stress and in immunity. Overall, these results support the application of SpPdp11 in the first life stages of S. senegalensis as an effective tool with the clear potential to benefit sole aquaculture.
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- 2018
12. A Diet‐Dependent Microbiota Profile Associated with Incident Type 2 Diabetes: From the CORDIOPREV Study
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Camargo, Antonio, primary, Vals‐Delgado, Cristina, additional, Alcala‐Diaz, Juan F., additional, Villasanta‐Gonzalez, Alejandro, additional, Gomez‐Delgado, Francisco, additional, Haro, Carmen, additional, Leon‐Acuña, Ana, additional, Cardelo, Magdalena P., additional, Torres‐Peña, Jose D., additional, Guler, Ipek, additional, Malagon, Maria M., additional, Ordovas, Jose M., additional, Perez‐Martinez, Pablo, additional, Delgado‐Lista, Javier, additional, and Lopez‐Miranda, Jose, additional
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- 2020
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13. Postprandial Lipemia Modulates Pancreatic Alpha-Cell Function in the Prediction of Type 2 Diabetes Development: The CORDIOPREV Study
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Pozuelo-Sanchez, Isabel, primary, Villasanta-Gonzalez, Alejandro, additional, Alcala-Diaz, Juan Francisco, additional, Vals-Delgado, Cristina, additional, Leon-Acuña, Ana, additional, Gonzalez-Requero, Anabel, additional, Yubero-Serrano, Elena Maria, additional, Luque, Raul Miguel, additional, Caballero-Villarraso, Javier, additional, Quesada, Ivan, additional, Ordovas, José María, additional, Pérez-Martinez, Pablo, additional, Roncero-Ramos, Irene, additional, and Lopez-Miranda, Jose, additional
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- 2020
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