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24 results on '"Villanueva-Segura, Olga Karina"'

1. Bacterial communities associated with Megalopyge opercularis (Smith) (Lepidoptera: Megalopygidae) : exploring poisonous lepidopterans

Author

Gómez-Govea, Mayra A., de Lourdes Ramírez-Ahuja, María, Castellanos-López, Luz M., Ruvalcaba-Ortega, Irene, Reyes-Cortes, Luisa M., de Jesús Trujillo-Rodríguez, Gerardo, Villanueva-Segura, Olga Karina, Martínez-Fierro, Margarita, Delgado-Enciso, Ivan, Ponce-García, Gustavo, Rodríguez-Garza, Ramón Gerardo, Flores-Suárez, Adriana E., and Rodríguez-Sánchez, Iram P.

Published
2022

2. miRNAsofAedesaegypti (Linnaeus 1762) conserved in six orders of the class Insecta

Author

Rodríguez-Sanchez, Iram Pablo, Saldaña-Torres, Daniel Rafael, Villanueva-Segura, Olga Karina, Garza-Rodriguez, Maria Lourdes, Gómez-Govea, Mayra A., Liang, Ghongwei, de Lourdes Ramírez-Ahuja, María, De La Luz Martinez-Fierro, Margarita, Delgado-Enciso, Ivan, Martinez-de-Villarreal, Laura E., Zhou, Yu, Flores-Suarez, Adriana E., Chen, Xi, Resendez-Pérez, Diana, Zhang, Chen-Yu, and Ponce-Garcia, Gustavo

Published
2021
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3. Free amino acid and acylcarnitine values in Ursus americanus Pallas 1780 (black bear) from Northeastern Mexico

Author

Abellan-Borja, Andres, primary, Rodriguez-Sanchez, Iram P., additional, Carrera-Treviño, Rogelio, additional, Villanueva-Segura, Olga Karina, additional, Zapata-Morin, Patricio Adrian, additional, Martinez-de-Villareal, Laura E., additional, Barboza-Aranda, Luis Javier, additional, Gomez-Govea, Mayra A., additional, Martinez-Fierro, Margarita L., additional, Delgado-Enciso, Ivan, additional, Ruiz-Ayma, Gabriel, additional, Gonzalez-Rojas, Jose Ignacio, additional, and Guzman-Velasco, Antonio, additional

Published
2023
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4. Bacterial Communities Associated with Megalopyge opercularis (Smith) (Lepidoptera: Megalopygidae): Exploring Poisonous Lepidopterans

Author

Gómez-Govea, Mayra A., primary, Lourdes Ramírez-Ahuja, María de, additional, Castellanos-López, Luz M., additional, Ruvalcaba-Ortega, Irene, additional, Reyes-Cortes, Luisa M., additional, Jesús Trujillo-Rodríguez, Gerardo de, additional, Villanueva-Segura, Olga Karina, additional, Martínez-Fierro, Margarita, additional, Delgado-Enciso, Ivan, additional, Ponce-García, Gustavo, additional, Rodríguez-Garza, Ramón Gerardo, additional, Flores-Suárez, Adriana E., additional, and Rodríguez-Sánchez, Iram P., additional

Published
2023
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5. Suppression of Midgut Microbiota Impact Pyrethroid Susceptibility in Aedes aegypti

Author

Gómez-Govea, Mayra A., primary, Ramírez-Ahuja, María de Lourdes, additional, Contreras-Perera, Yamili, additional, Jiménez-Camacho, Armando J., additional, Ruiz-Ayma, Gabriel, additional, Villanueva-Segura, Olga Karina, additional, Trujillo-Rodríguez, Gerardo de Jesús, additional, Delgado-Enciso, Iván, additional, Martínez-Fierro, Margarita L., additional, Manrique-Saide, Pablo, additional, Puerta-Guardo, Henry, additional, Flores-Suárez, Adriana E., additional, Ponce-García, Gustavo, additional, and Rodríguez-Sánchez, Iram P., additional

Published
2022
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7. Microbiota Profile of Melanaphis sacchari1: A Destructive Pest of Sorghum bicolor

Author

Gómez-Govea, Mayra A., primary, Bravo-Acosta, Alondra M., additional, Ramírez-Ahuja, María De Lourdes, additional, Villanueva-Segura, Olga Karina, additional, Ruiz-Aymá, Gabriel, additional, Guzmán-Velasco, Antonio, additional, González-Rojas, José Ignacio, additional, Martínez-Fierro, Margarita, additional, Enciso, Ivan Delgado, additional, Ramírez-Valles, Eda G., additional, Martínez-Dávila, Jorge A., additional, Cruz-Fierro, Norma, additional, Trujillo-Murillo, Karina Del C., additional, Ponce-García, Gustavo, additional, Flores-Suárez, Adriana E., additional, and Rodríguez-Sánchez, Iram P., additional

Published
2021
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8. miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta

Author

Rodríguez-Sanchez, Iram Pablo, primary, Saldaña-Torres, Daniel Rafael, additional, Villanueva-Segura, Olga Karina, additional, Garza-Rodriguez, Maria Lourdes, additional, Gómez-Govea, Mayra A., additional, Liang, Ghongwei, additional, de Lourdes Ramírez-Ahuja, María, additional, De La Luz Martinez-Fierro, Margarita, additional, Delgado-Enciso, Ivan, additional, Martinez-de-Villarreal, Laura E., additional, Zhou, Yu, additional, Flores-Suarez, Adriana E., additional, Chen, Xi, additional, Resendez-Pérez, Diana, additional, Zhang, Chen-Yu, additional, and Ponce-Garcia, Gustavo, additional

Published
2021
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9. Molecular Description of Proclotting Enzyme: A Power Tool for Insect Biological Control of Aedes aegypti L.1

Author

Villanueva-Segura, Olga Karina, primary, Gómez-Govea, Mayra A., additional, Garza-Veloz, Idalia, additional, González-Alvarez, Rafael, additional, Carrillo-Gaytán, Diego, additional, Ramírez-Valles, Eda G., additional, Martínez-Dávila, Jorge A., additional, Cruz-Fierro, Norma, additional, Trujillo-Murillo, Karina del C., additional, Martínez-Fierro, Margarita L., additional, Delgado-Enciso, Iván, additional, Rodríguez-Sánchez, Iram Pablo, additional, and Guzmán-Velasco, Antonio, additional

Published
2020
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10. Molecular Description of Proclotting Enzyme: A Power Tool for Insect Biological Control of Aedes aegypti L..

Author

Villanueva-Segura, Olga Karina, Gómez-Govea, Mayra A., Garza-Veloz, Idalia, González-Alvarez, Rafael, Carrillo-Gaytán, Diego, Ramírez-Valles, Eda G., Martínez-Dávila, Jorge A., Cruz-Fierro, Norma, Trujillo-Murillo, Karina del C., Martínez-Fierro, Margarita L., Delgado-Enciso, Iván, Rodríguez-Sánchez, Iram Pablo, and Guzmán-Velasco, Antonio

Subjects
*BIOLOGICAL control of insects, *AEDES aegypti, *POWER tools, *ENZYMES, *EMBRYOLOGY, *PROTEOLYTIC enzymes, *PROTEINASES
Abstract

Proclotting enzymes (PCE) are a serine protease-containing CLIP domain and play roles in development of embryonic and innate immune response. Little is known about PCE in mosquitoes, but only three melanization regulatory modules that involve proteases with the CLIP domain have been reported. We cloned the PCE gene and reported the nucleotide sequence encoding a proclotting enzyme in Aedes aegypti L. We did in-silico protein translation, alignment with proclotting proteinase sequences of the other species of different taxonomic orders, and phylogenetic analysis where PCE in Ae. aegypti fit the hypotheses of positive or adaptive evolution. The fundamental information could be applied in strategies for biological control of medically important insects. [ABSTRACT FROM AUTHOR]

Published
2020
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12. Identification of Four Isoforms of Esterase-5A fromCulex quinquefasciatusSay

Author

Gómez-Govea, Mayra A., primary, Contreras-Perera, Yamili, additional, Pérez-Ibave, Diana Cristina, additional, González-Alvarez, Rafael, additional, Garza-Rodríguez, María de Lourdes, additional, Fierro, Margarita de la Luz Martínez, additional, Villarreal, Laura E. Martínez de, additional, Delgado-Enciso, Iván, additional, Flores-Suárez, Adriana E., additional, Gutierrez, Eugenia Cisneros, additional, Olvera, Gabriela González, additional, Davila, Jorge Alberto Martínez, additional, Veloz, Idalia Garza, additional, Cruz-Fierro, Norma, additional, Ponce-García, Gustavo, additional, Villanueva-Segura, Olga Karina, additional, and Rodriguez-Sanchez, Irám Pablo, additional

Published
2018
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13. Genomic Mining, Evolution Analysis, and Structural Characterization of the Sepik Virus

Author

Saldaña-Torres, Daniel Rafael, primary, López-Rodríguez, Martha Patricia, additional, Trujillo-Rodríguez, Gerardo de Jesús, additional, Villanueva-Segura, Olga Karina, additional, Ponce-Garcia, Gustavo, additional, González-Alvarez, Rafael, additional, Delgado-Enciso, Iván, additional, Martinez-Fierro, Margarita de La Luz, additional, Martínez-De-Villarreal, Laura Elia, additional, Garza-Guajardo, Raquel, additional, Barboza-Quintana, Oralia, additional, Flores-Suárez, Adriana Elizabeth, additional, and Rodríguez-Sánchez, Irám Pablo, additional

Published
2018
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14. Profiles of Amino Acids and Acylcarnitines Related with Insecticide Exposure in Culex quinquefasciatus (Say)

Author

Martin-Park, Abdiel, primary, Gomez-Govea, Mayra A., additional, Lopez-Monroy, Beatriz, additional, Treviño-Alvarado, Víctor Manuel, additional, Torres-Sepúlveda, María del Rosario, additional, López-Uriarte, Graciela Arelí, additional, Villanueva-Segura, Olga Karina, additional, Ruiz-Herrera, María del Consuelo, additional, Martinez-Fierro, Margarita de la Luz, additional, Delgado-Enciso, Ivan, additional, Flores-Suárez, Adriana E., additional, White, Gregory S., additional, Martínez de Villarreal, Laura E., additional, Ponce-Garcia, Gustavo, additional, Black, William C., additional, and Rodríguez-Sanchez, Irám Pablo, additional

Published
2017
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15. Mining the Alkhumra Hemorrhagic Fever Virus Genomes in 2015

Author

Ruiz-Aymá, Gabriel, primary, Tovar-Herrera, Omar Eduardo, additional, González-Alvarez, Rafael, additional, Villanueva-Segura, Olga Karina, additional, Gómez-Govea, Mayra A., additional, Fajardo-Ramirez, Oscar Raúl, additional, Zamudio-Osuna, Michelle de Jesús, additional, Martínez-de-Villarreal, Laura Elia, additional, Córdova, Carlos Andrés Urbina, additional, Garza-Guajardo, Raquel, additional, Ponce-Garcia, Gustavo, additional, Barboza-Quintana, Oralia, additional, Flores-Suarez, Adriana Elizabeth, additional, and Rodríguez-Sánchez, Irám Pablo, additional

Published
2016
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16. Caracterización molecular del receptor de la Vitelogenia en Bactericera cockerelli [Sulc, 1909] (hemiptera:triozidae)

Author

Villanueva Segura, Olga Karina and Villanueva Segura, Olga Karina

Abstract

El permanente del tomate, la enfermedad de la punta morada, y el “Zebra chip” sonenfermedades que ocasionan daños considerables en los cultivos de tomate y papa,respectivamente. El agente causal es trasmitido por injerto o por interacción con plagas de insectos de la especie Bactericera cockerelli Sulc. En México en años recientes, B.cockerelli es considerada como plaga primaria, y es común que se realicen aplicaciones de insecticidas durante la etapa de cultivo. Se ha manifestado alarma debido a que hayfallas en el control por medios químicos, aunque puede atribuirse el problema a deficiente calibración de equipos de aplicación, baja cobertura de la aspersión en el follaje o la expresión fenotípica de la resistencia a insecticidas. Esto último, hace evidente la necesidad del desarrollo de métodos de control alternativos para este tipo de plaga. Recientemente, la genética es utilizada para obtener el máximo beneficio de ella y obtener blancos específicos de regulación en muy poco tiempo, aunado a esto, la relación que existe entre la reproducción y el proceso de vitelogénesis hace de esto una alternativa potencial para el control de insectos. Así, acoplando ambos conocimientos se podría aplicar una estrategia genética adecuada a largo plazo para alterar la expresión de los transcritos codificantes en el proceso de vitelogénesis reduciendo de esta forma la población plaga y la propagación de las enfermedades. El objetivo de este trabajo estuvo encaminado a caracterizar parcialmente y cuantificar el receptor de vitelogenina en B. cockerelli. Para llevar a cabo este estudio, se cubrió los siguientes objetivos particulares; se buscó la secuencia del receptor de vitelogenina por dos estrategias, con secuencias publicadas de otras especies de insectos y por el transcriptoma de B. cockerelli. La caracterización parcial del receptor se hizo a través de las secuencias nucleotídicas obtenidas por PCR en punto final, y secuenciación por electroforesis capilar. La cuantificació

Published
2015

17. Molecular Cloning, Sequence Analysis, and Gene Expression of the Circadian Clock GenePeriodinCulex quinquefasciatusSay (Diptera: Culicidae)

Author

González-Alvarez, Rafael, primary, Villanueva-Segura, Olga Karina, additional, Ponce-García, Gustavo, additional, Martínez-Fierro, Margarita de la Luz, additional, Delgado-Enciso, Iván, additional, Flores-Suárez, Adriana Elizabeth, additional, Garza-Guajardo, Raquel, additional, Osuna, Michelle de Jesús Zamudio, additional, Barrera-Saldaña, Hugo Alberto, additional, Barboza-Quintana, Oralia, additional, Martínez-de-Villarreal, Laura Elia, additional, Guzmán-Velasco, Antonio, additional, and Rodríguez-Sánchez, Irám Pablo, additional

Published
2015
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18. PCR- RFLP for Identification of Bluetongue Virus Serotypes

Author

Bernal-Silva, Sofía, primary, Villanueva-Segura, Olga Karina, additional, Lugo-Trampe, Ángel, additional, Trujillo-Murillo, Karina del Carmen, additional, Ponce-García, Gustavo, additional, Favela-Lara, Susana, additional, Delgado-Encizo, Ivan, additional, Martínez-de-Villarreal, Laura E., additional, Garza-Guajardo, Raquel, additional, Barboza-Quintana, Oralia, additional, González-Alvarez, Rafael, additional, Flores-Suarez, Adriana E., additional, and Rodríguez-Sánchez, Irám Pablo, additional

Published
2015
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19. Molecular Cloning and Characterization of the Circadian ClockTimelessGene inCulex quinquefasciatusSay (Diptera: Culicidae)

Author

Rodriguez-Sanchez, Irám Pablo, primary, Villanueva-Segura, Olga Karina, additional, Gonzalez-Alvarez, Rafael, additional, Flores-Suarez, Adriana Elizabeth, additional, Garza-Rodriguez, María de Lourdes, additional, Delgado-Enciso, Iván, additional, Martinez-de-Villarreal, Laura Elia, additional, Castillo, Ricardo Canales del, additional, Favela-Lara, Susana, additional, Garza-Guajardo, Raquel, additional, Lugo-Trampe, Ángel, additional, Trujillo-Murillo, Karina del Carmen, additional, Barboza-Quintana, Oralia, additional, Gonzalez-Rojas, José Ignacio, additional, Barrera-Saldaña, Hugo Alberto, additional, and Ponce-Garcla, Gustavo, additional

Published
2015
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21. Efecto del motivo WRPW en la actividad funcional de antennapedia en Drosophila melanogaster

Author

Villanueva Segura, Olga Karina and Villanueva Segura, Olga Karina

Abstract

Los genes homeóticos permiten el control genético del desarrollo de los diferentes organismos en la especificación del plan corporal. Estos genes presentan dos secuencias altamente conservadas: el homeobox que codifica a un péptido de 60 aminoácidos conocido como homeodominio (HD) que es el sitio de unión al DNA y el tetrapéptidoYPWM que se ha involucrado en interacciones proteína-proteína. Estos dominios estan presentes en la homeoproteína Antennapedia (Antp) que es un factor transcripcional que controla la formación de los segmentos torácicos en Drosophila melanogaster. Similarmente, la proteína Hairy contiene el motivo WRPW que es capaz de conferir actividad represora debido al reclutamiento del corepresor Groucho. Debido a que la actividad funcional de las diferentes proteínas homeóticas esta dada por un balance regulado por los mecanismos de activación/represión de las homeoproteínas para la regulación de los genes blanco, en esta tesis se realizó la adición del motivo de represión transcripcional WRPW, en el extremo C-terminal de Antp para determinar el efecto de un motivo de represión adicional en la actividad funcional de Antp durante el desarrollo embrionario y en la actividad de un gen blanco en D. melanogaster. Para cumplir con los objetivos se llevó a cabo la clonación de las secuencias nucleotídicas codificantes a Antp y sus mutantes con la fusión a WRPW en el vector pUAST. Los vectores recombinantes fueron microinyectados en embriones de moscas w1118 para la obtención de las líneas transgénicas, en las que se analizó la expresión ectópica de Antp quimérica usando el sistema binario GAL4-UAS mediante cruzas con la línea Hs-GAL4. Las larvas producto de las cruzas fueron analizadas para la determinación de los efectos fenotípicos producidos por la inserción AntpWRPW. El análisis de activación transcripcional del gen blanco tsh se realizó mediante cruzas genéticas con la línea patched-GAL4 para detectar el efecto del motivo WRPW en la actividad funcional d

Published
2007

22. Molecular Cloning, Sequence Analysis, and Gene Expression of the Circadian Clock Gene Period in Culex quinquefasciatus Say (Diptera: Culicidae).

Author

González-Alvarez, Rafael, Villanueva-Segura, Olga Karina, Ponce-García, Gustavo, de la Luz Martínez-Fierro, Margarita, Delgado-Enciso, Iván, Flores-Suárez, Adriana Elizabeth, Garza-Guajardo, Raquel, de Jesús Zamudio Osuna, Michelle, Barrera-Saldaña, Hugo Alberto, Barboza-Quintana, Oralia, Martínez-de-Villarreal, Laura Elia, Guzmán-Velasco, Antonio, and Rodríguez-Sánchez, Irám Pablo

Subjects
*CULEX quinquefasciatus, *MOLECULAR cloning, *CIRCADIAN rhythms, *MOSQUITO genetics, *GENE expression, *INSECT genetics, *RNA sequencing
Abstract

In prokaryotes and eukaryotes, the Period (PER) gene is important for the circadian clock that regulates some rhythmic biochemical, physiological, and behavioral processes. PER messenger amplification was done by RT-PCR from southern house mosquito, Culex quinquefasciatus Say, larvae collected at three locations in the State of Nuevo Leon, northeastern Mexico. The amplified products were cloned, sequenced, and compared/analyzed with those of other species of mosquitoes. A 3,093-bp amplified product was synthesized by PCR, which is the complete coding DNA sequence (3,027 bp) of PER encoding an open reading frame of 996 amino acid residues and much smaller than the orthologous in other mosquitoes analyzed. No other simultaneous amplification was visualized; thus, possible alternative splicings were discarded or at least not detected by the method. Because PER is a widely conserved gene in prokaryotes and eukaryotes and maintaining colonies of southern house mosquitoes is relativity easy, determining the sequence of the gene in the mosquito provides the possibility of using the gene as a model and generating data on the role in the circadian clock. [ABSTRACT FROM AUTHOR]

Published
2015

23. Molecular Cloning and Characterization of the Circadian Clock Timeless Gene in Culex quinquefasciatus Say (Diptera: Culicidae).

Author

Rodriguez-Sanchez, Irám Pablo, Villanueva-Segura, Olga Karina, Gonzalez-Alvarez, Rafael, Flores-Suarez, Adriana Elizabeth, de Lourdes Garza-Rodriguez, María, Delgado-Enciso, Iván, Martinez-de-Villarreal, Laura Elia, del Castillo, Ricardo Canales, Favela-Lara, Susana, Garza-Guajardo, Raquel, Lugo-Trampe, Ángel, del Carmen Trujillo-Murillo, Karina, Barboza-Quintana, Oralia, Gonzalez-Rojas, José Ignacio, Barrera-Saldaña, Hugo Alberto, and Ponce-Garcia, Gustavo

Subjects
*MOLECULAR cloning, *CULEX quinquefasciatus, *CIRCADIAN rhythms, *MOSQUITO genetics, *ANTISENSE DNA
Abstract

In eukaryotes, the timeless (TIM) gene plays a fundamental role in the control of the circadian clock by regulating several biochemical, physiological, and behavioral processes. RT-PCR was used to amplify TIM mRNA from larval southern house mosquito, Culex quinquefasciatus Say, from three locations in the State of Nuevo Leon in northeastern Mexico. The 3062 bp TIM cDNA was assembled from three overlapping PCRs that were cloned and sequenced. DNA sequence analysis used amplified cDNA fragments to complete coding the DNA sequence (2991 bp). Tim ORF consisting of 996 codons was shorter than the orthologous genes from other mosquito species. A series of deletions in the core sequence of TIM were detected. No other amplification products were visualized, and thus, the possibility of alternative spliced mRNA species was discarded. Because TIM is a widely conserved gene in eukaryotes, and maintaining colonies of Cx. quinquefasciatus is easy, determining the sequence of the TIM gene in the mosquito provides the possibility of its use as a model for circadian clock experimentation. [ABSTRACT FROM AUTHOR]

Published
2015
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24. Identification of Four Isoforms of Esterase-5A from Culex quinquefasciatus Say

Author

Gómez-Govea, Mayra A., Contreras-Perera, Yamili, Pérez-Ibave, Diana Cristina, González-Alvarez, Rafael, Garza-Rodríguez, María de Lourdes, Fierro, Margarita de la Luz Martínez, Villarreal, Laura E. Martínez de, Delgado-Enciso, Iván, Flores-Suárez, Adriana E., Gutierrez, Eugenia Cisneros, Olvera, Gabriela González, Davila, Jorge Alberto Martínez, Veloz, Idalia Garza, Cruz-Fierro, Norma, Ponce-García, Gustavo, Villanueva-Segura, Olga Karina, and Rodriguez-Sanchez, Irám Pablo

Published
2018
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