15 results on '"Villalobos Reyes, Salvador"'
Search Results
2. Diagnosis of Plant Viruses Using NGS to Support Virus Management and Tomato Breeding
- Author
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González-Pérez, Enrique, primary, Chiquito-Almanza, Elizabeth, additional, Villalobos-Reyes, Salvador, additional, Canul-Kú, Jaime, additional, and Anaya-López, José Luis, additional
- Published
- 2024
- Full Text
- View/download PDF
3. Diagnosis and Characterization of Plant Viruses Using HTS to Support Virus Management and Tomato Breeding.
- Author
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González-Pérez, Enrique, Chiquito-Almanza, Elizabeth, Villalobos-Reyes, Salvador, Canul-Ku, Jaime, and Anaya-López, José Luis
- Subjects
TOMATO breeding ,PLANT viruses ,TOBACCO mosaic virus ,TOMATO diseases & pests ,GENETIC variation ,MIXED infections ,TOMATOES - Abstract
Viral diseases pose a significant threat to tomato crops (Solanum lycopersicum L.), one of the world's most economically important vegetable crops. The limited genetic diversity of cultivated tomatoes contributes to their high susceptibility to viral infections. To address this challenge, tomato breeding programs must harness the genetic resources found in native populations and wild relatives. Breeding efforts may aim to develop broad-spectrum resistance against the virome. To identify the viruses naturally infecting 19 advanced lines, derived from native tomatoes, high-throughput sequencing (HTS) of small RNAs and confirmation with PCR and RT-PCR were used. Single and mixed infections with tomato mosaic virus (ToMV), tomato golden mosaic virus (ToGMoV), and pepper huasteco yellow vein virus (PHYVV) were detected. The complete consensus genomes of three variants of Mexican ToMV isolates were reconstructed, potentially forming a new ToMV clade with a distinct 3' UTR. The absence of reported mutations associated with resistance-breaking to ToMV suggests that the Tm-1, Tm-2, and Tm-2
2 genes could theoretically be used to confer resistance. However, the high mutation rates and a 63 nucleotide insertion in the 3' UTR, as well as amino acid mutations in the ORFs encoding 126 KDa, 183 KDa, and MP of Mexican ToMV isolates, suggest that it is necessary to evaluate the capacity of these variants to overcome Tm-1, Tm-2, and Tm-22 resistance genes. This evaluation, along with the characterization of advanced lines using molecular markers linked to these resistant genes, will be addressed in future studies as part of the breeding strategy. This study emphasizes the importance of using HTS for accurate identification and characterization of plant viruses that naturally infect tomato germplasm based on the consensus genome sequences. This study provides crucial insights to select appropriate disease management strategies and resistance genes and guide breeding efforts toward the development of virus-resistant tomato varieties. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
4. Nutrient dynamics of macro and micronutrients in coriander
- Author
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González Pérez, Enrique, Villalobos Reyes, Salvador, Núñez Colín, Carlos A., González Pérez, Enrique, Villalobos Reyes, Salvador, and Núñez Colín, Carlos A.
- Abstract
The information about the coriandernutrient management is scarce. This study aims to determine the dynamics of macro and micronutrients in coriander plants during its different phenological stages and growth seasons. The concentration of macro and micronutrients in seven phenological stages and some agronomic components were determined. The most required macronutrients were N (42.1-57.5 g plant-1), followed by K (42.5-53.8 g plant-1) and Ca (7.7-25.9 g plant-1). Mn (105.1-381 mg plant-1) and Fe (49.8-194.4 mg plant-1) were the micronutriments required in the hi-ghest amounts. The best plant height (56.4cm), fresh weight (226.7 g pta-1) and dry weight (30.7 g pta-1) was recorded in autumn-winter (A-W) (P ≤ 0.05), while oil content was from 7.62 to 8.12%, La información sobre el manejo nutrimental del cilantro es escasa. Así, el objetivo del estudio fue determinar la dinámica de macro y micro-nutrimentos en plantas de cilantro en diferentes etapas fenológicas y épocas de siembra. La concentración de macro y micro nutrimentos en siete etapas fenológicas y algunos componentes agronómicos fueron determinados. El macro-nutrimento más requerido fue N (42.1-57.5 g plant-1), seguido de K (42.5-53.8 g plant-1) y de Ca (7.7-25.9 g plant-1), y los micro-nutrimentos requeridos en altas cantidades fueron Mn (105.1–381 mg plant-1) y Fe (49.8–194.4 mg plant-1). La mayor altura de planta (56.4 cm), peso fresco (226.7 g pta-1) y peso seco (30.7 g pta-1) fue registrada en otoño-invierno (O-I) (P ≤ 0.05), mientras que el contenido de aceite fue de 7.62 a 8.12%
- Published
- 2023
5. Valoracion de germoplasma de tomate de cascara cultivado en el estado de Morelos, Mexico
- Author
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Canul-Ku, Jaime, González-Pérez, Enrique, Villalobos-Reyes, Salvador, Javier Barrios-Gómez, Edwin, and Rangel-Estrada, Sandra Eloísa
- Published
- 2017
6. Variabilidad genotípica de aislados de Phytophthora capsici en Guanajuato
- Author
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Guerrero Aguilar, Brenda Zulema, Pons-Hernández, José Luis, González Chavira, Mario M., González Pérez, Enrique, Villalobos Reyes, Salvador, Rodríguez-Guerra, Raúl, Guerrero Aguilar, Brenda Zulema, Pons-Hernández, José Luis, González Chavira, Mario M., González Pérez, Enrique, Villalobos Reyes, Salvador, and Rodríguez-Guerra, Raúl
- Abstract
Phytophthora capsiciisthe causative agent of ‘chili pepperwilt’one of the most important diseases of this crop. To establish efficient control strategies,it is necessary to know the diversity of the pathogen, for this, this research was carried out,whose objective was to determine the degree of genetic diversity of 30 monozoosporic isolates obtained from pepper(Capsicum annuumL.) from six municipalitiesin the state of Guanajuato, and two reference strains. The genetic variation was determined by the AFLP technique. The similarity found between the isolates ranges from 76 to 95%. The results obtained indicate that, within the population,groups form based on the localities of isolation. No clonal organisms were found, so the 32 isolates analyzed were unique genotypes, and no correlation was found between groups defined by molecular markers and virulence, type of mating or response to metalaxyl, Phytophthora capsici, es el agente causal de la ‘marchitez del chile’una de las enfermedades más importantes de este cultivo. Para establecer estrategias de control eficientes es necesario el conocimiento de la diversidad del patógeno, para esto, se realizó esta investigación cuyo objetivo fue determinar el grado de diversidad genética de 30aislados monozoospóricosobtenidos de chile (Capsicum annuumL.) de seis municipios del estado de Guanajuato, y dos cepas de referencia. La variación genética se determinó mediante la técnica de AFLP (en español: polimorfismo en la longitud de fragmentos amplificados). La similitud encontrada entre los aislados va de 76 a 95%. Los resultados obtenidos indican que dentro de la población se forman grupobasados en las localidades de aislamiento. No se encontraron organismos clónales por lo que los 32aislamientos analizados fueron genotipos únicos, yno se encontró ninguna correlación entre grupos definidos por marcadores moleculares y virulencia, tipo de apareamiento o respuesta a metalaxil
- Published
- 2022
7. Variabilidad genotípica de aislados de Phytophthora capsici en Guanajuato
- Author
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Guerrero-Aguilar, Brenda Zulema, primary, Pons-Hernández, José Luis, additional, González-Chavira, Mario Martin, additional, González-Pérez, Enrique, additional, Villalobos-Reyes, Salvador, additional, and Rodríguez Guerra, Raúl, additional
- Published
- 2022
- Full Text
- View/download PDF
8. Nutrient dynamics of macro and micronutrients in coriander.
- Author
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González-Pérez, Enrique, Villalobos-Reyes, Salvador, and Nuñez-Colin, Carlos Alberto
- Subjects
- *
MICRONUTRIENTS , *CORIANDER - Abstract
The information about the coriander nutrient management is scarce. This study aims to determine the dynamics of macro and micronutrients in coriander plants during its different phenological stages and growth seasons. The concentration of macro and micronutrients in seven phenological stages and some agronomic components were determined. The most required macronutrients were N (42.1-57.5 g plant-1), followed by K (42.5-53.8 g plant-1) and Ca (7.7-25.9 g plant-1). Mn (105.1-381 mg plant-1) and Fe (49.8-194.4 mg plant-1) were the micronutriments required in the highest amounts. The best plant height (56.4cm), fresh weight (226.7 g pta-1) and dry weight (30.7 g pta-1) was recorded in autumn-winter (A-W) (P ≤ 0.05), while oil content was from 7.62 to 8.12%. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
9. Variabilidad fenotípica de aislados de Phytophthora capsici en Guanajuato
- Author
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González Chavira, Mario Martín, primary, Pons-Hernández, José Luis, additional, Guerrero-Aguilar, Brenda Zulema, additional, González-Pérez, Enrique, additional, Villalobos-Reyes, Salvador, additional, and Muñoz-Sánchez, Claudia Ivonne, additional
- Published
- 2020
- Full Text
- View/download PDF
10. Variabilidad genotípica de aislados de Phytophthora capsici en Guanajuato.
- Author
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Zulema Guerrero-Aguilar, Brenda, Luis Pons-Hernández, José, Martin González-Chavira, Mario, González-Pérez, Enrique, Villalobos-Reyes, Salvador, and Rodríguez Guerra, Raúl
- Subjects
PEPPERS ,PHYTOPHTHORA capsici ,GENETIC variation ,PLANT diseases ,BASE isolation system ,CAPSICUM annuum ,HOT peppers - Abstract
Copyright of Revista Mexicana de Ciencias Agrícolas is the property of Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
- Full Text
- View/download PDF
11. Caracterización genética, química y agronómica de líneas avanzadas de tomate de cáscara
- Author
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González-Chavira, Mario Martin, Guzmán-Maldonado, Salvador Horacio, Pons-Hernández, José Luis, Villalobos-Reyes, Salvador, and Gónzalez-Pérez, Enrique
- Subjects
molecular markers ,production potential ,marcadores moleculares ,producción potencial ,functional components ,diversidad genética ,genetic diversity ,Physalis ixocarpa ,compuestos funcionales - Abstract
Introduction. Knowledge about the genetic, chemical and morphological diversity that exists between individuals and populations is very useful in breeding programs, because it facilitates the organization of the material and the appropriate selection of superior genotypes for the development of an improved population. Objective. The aim of this research was to make a genetic, chemical and agronomic characterization in twenty advanced husk tomato lines (Physalis ixocarpa Brot.), belonging to the vegetable breeding program of the Campo Experimental Bajío (CE-Bajío), of the Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), México. Materials and methods. During the spring-summer and autumn-winter 2017, the genetic variability was quantified with molecular markers of DNA, type AFLP (Amplified Fragment Length Polymorphisms), likewise, the phenolic and tomatidine compounds in the fruit were determined, and agronomic traits like germination percentage, number of fruits per plant, weight of the fruit, equatorial and polar diameter per fruit, and fruit yield were determined. A general average of similarity between the genotypes of 0.86 was obtained. Results. A general similitude mean between genotypes of 0.86 was obteined. According to the genetic relationships, a geographic pattern was identified and genotypes 4 and 70 were detected as possible progenitors of improved hybrids. Chemical diversity indicated that the fruits flavonoids content in L-86 was the highest (51.1mg EAG/100g), phenols (396.8 mg EAG/100 g) and anthocyanin’s (7.22 mg EAG/100g) for L-182 and tannins (188.4 mg EAG/100 g) for L-97, while tomatidine (2.23-3.81 mg EAG/100 g) was higher in green fruits than purple fruits. The agronomic results indicate that the lines fruit yield ranged from 11.4 to 47.6 t/ha, the 20% of the lines has a superior yield than the national mean yield (40 t/ha), The L-37 was noticeable, since it has the highest fruit yield with 47.6 t/ha, and has the highest number of fruits number per plant, equatorial and polar diameters, and germination rate (93.3%). Conclusion. Based on the results the lines 37, 25, 27 and 167 are positioned as lines with potential for commercial use and as parental lines. Introducción. Conocer la diversidad a nivel genético, químico y morfológico que existe entre individuos y poblaciones, es de gran utilidad en los programas de mejoramiento genético, ya que facilita la organización del material y la selección adecuada de genotipos superiores para el desarrollo de una población mejorada. Objetivo. El objetivo del presente trabajo fue caracterizar genética, química y agronómicamente líneas avanzadas de tomate de cáscara (Physalis ixocarpa Brot.), del programa de mejoramiento de hortalizas del Campo Experimental Bajío (CE-Bajío) del Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), México. Materiales y métodos. Durante la temporada primavera-verano y otoño-invierno de 2017, se cuantificó la variabilidad genética con marcadores moleculares de ADN de tipo AFLP (Amplified Fragment Length Polymorphism); se determinaron los compuestos fenólicos y tomatidina en fruto, y en lo agronómico el porcentaje de germinación, número de frutos por planta, peso del fruto, diámetro ecuatorial y polar, y el rendimiento. Resultados. Se obtuvo un promedio general de similitud entre los genotipos de 0,86. De acuerdo con las relaciones genéticas, se detectaron las líneas 4 y 70 como posibles progenitores de híbridos y un patrón geográfico en los agrupamientos. La diversidad química indicó que el mayor contenido de flavonoides (51,1 mg EAG/100 g) se presentó en la L-86, de fenoles y antocianinas (396, 8 y 7,22 mg EAG/100 g, resp.) en la L-182, y de taninos (188,4 mg EAG/100 g) en la L-97, mientras que la tomatidina fue mayor en frutos verdes (2,23-3,81 mg EAG/100 g) que en morados. El intervalo de rendimiento de las líneas fue de 11,4 a 47,6 t/ha, donde el 20% de las líneas superaron la media de rendimiento nacional (40 t/ha). Se destacó la L-37 con 47,6 t/ha, que además produjo mayor número de frutos por planta, diámetro ecuatorial y polar, y una tasa de germinación de 93,3%. Conclusión. Las líneas 37, 25, 27 y 167, tienen potencial para ser validadas para uso comercial y como líneas parentales.
- Published
- 2019
12. Caracterización genética, química y agronómica de líneas avanzadas de tomate de cáscara
- Author
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González Chavira, Mario M., Guzmán Maldonado, Salvador Horacio, Pons Hernández, J. Luis, Villalobos Reyes, Salvador, González Pérez, Enrique, González Chavira, Mario M., Guzmán Maldonado, Salvador Horacio, Pons Hernández, J. Luis, Villalobos Reyes, Salvador, and González Pérez, Enrique
- Abstract
Introducción. Conocer la diversidad a nivel genético, químico y morfológico que existe entre individuos y poblaciones, es de gran utilidad en los programas de mejoramiento genético, ya que facilita la organización del material y la selección adecuada de genotipos superiores para el desarrollo de una población mejorada. Objetivo. El objetivo del presente trabajo fue caracterizar genética, química y agronómicamente líneas avanzadas de tomate de cáscara (Physalis ixocarpa Brot.), del programa de mejoramiento de hortalizas del Campo Experimental Bajío (CE-Bajío) del Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), México. Materiales y métodos. Durante la temporada primavera-verano y otoño-invierno de 2017, se cuantificó la variabilidad genética con marcadores moleculares de ADN de tipo AFLP (Amplified Fragment Length Polymorphism); se determinaron los compuestos fenólicos y tomatidina en fruto, y en lo agronómico el porcentaje de germinación, número de frutos por planta, peso del fruto, diámetro ecuatorial y polar, y el rendimiento. Resultados. Se obtuvo un promedio general de similitud entre los genotipos de 0,86. De acuerdo con las relaciones genéticas, se detectaron las líneas 4 y 70 como posibles progenitores de híbridos y un patrón geográfico en los agrupamientos. La diversidad química indicó que el mayor contenido de flavonoides (51,1 mg EAG/100 g) se presentó en la L-86, de fenoles y antocianinas (396, 8 y 7,22 mg EAG/100 g, resp.) en la L-182, y de taninos (188,4 mg EAG/100 g) en la L-97, mientras que la tomatidina fue mayor en frutos verdes (2,23-3,81 mg EAG/100 g) que en morados. El intervalo de rendimiento de las líneas fue de 11,4 a 47,6 t/ha, donde el 20% de las líneas superaron la media de rendimiento nacional (40 t/ha). Se destacó la L-37 con 47,6 t/ha, que además produjo mayor número de frutos por planta, diámetro ecuatorial y polar, y una tasa de germinación de 93,3%. Conclusión. Las líneas 37, 25, 27 y 167, tienen poten, Introduction. Knowledge about the genetic, chemical and morphological diversity that exists between individuals and populations is very useful in breeding programs, because it facilitates the organization of the material and the appropriate selection of superior genotypes for the development of an improved population. Objective. The aim of this research was to make a genetic, chemical and agronomic characterization in twenty advanced husk tomato lines (Physalis ixocarpa Brot.), belonging to the vegetable breeding program of the Campo Experimental Bajío (CE-Bajío), of the Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), México. Materials and methods. During the spring-summer and autumn-winter 2017, the genetic variability was quantified with molecular markers of DNA, type AFLP (Amplified Fragment Length Polymorphisms), likewise, the phenolic and tomatidine compounds in the fruit were determined, and agronomic traits like germination percentage, number of fruits per plant, weight of the fruit, equatorial and polar diameter per fruit, and fruit yield were determined. A general average of similarity between the genotypes of 0.86 was obtained. Results. A general similitude mean between genotypes of 0.86 was obteined. According to the genetic relationships, a geographic pattern was identified and genotypes 4 and 70 were detected as possible progenitors of improved hybrids. Chemical diversity indicated that the fruits flavonoids content in L-86 was the highest (51.1mg EAG/100g), phenols (396.8 mg EAG/100 g) and anthocyanin’s (7.22 mg EAG/100g) for L-182 and tannins (188.4 mg EAG/100 g) for L-97, while tomatidine (2.23-3.81 mg EAG/100 g) was higher in green fruits than purple fruits. The agronomic results indicate that the lines fruit yield ranged from 11.4 to 47.6 t/ha, the 20% of the lines has a superior yield than the national mean yield (40 t/ha), The L-37 was noticeable, since it has the highest fruit yield with 47.6 t/ha, and has the h
- Published
- 2019
13. Variabilidad fenotípica de aislados de Phytophthora capsici en Guanajuato.
- Author
-
Luis Pons-Hernández, José, Zulema Guerrero-Aguilar, Brenda, Martin González-Chavira, Mario, González-Pérez, Enrique, Villalobos-Reyes, Salvador, and Ivonne Muñoz-Sánchez, Claudia
- Abstract
Copyright of Revista Mexicana de Ciencias Agrícolas is the property of Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2020
14. Componentes agronómicos y diversidad en el patrón de ácidos grasos en líneas avanzadas de cilantro
- Author
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Guzmán Maldonado, Salvador Horacio, primary, Villalobos Reyes, Salvador, additional, Escobedo López, Diana, additional, and González Pérez, Enrique, additional
- Published
- 2018
- Full Text
- View/download PDF
15. Determinación de la relación pez planta en la producción de tomate (Licopersicum sculentum L.) en sistema de acuaponia
- Author
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Villalobos-Reyes, Salvador, primary and González-Pérez, Enrique, additional
- Published
- 2017
- Full Text
- View/download PDF
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